TY  - JOUR
AU  - Francuski, Jelena
AU  - Radovanović, Anita
AU  - Andrić, Nenad
AU  - Krstić, Vanja
AU  - Bogdanović, Dragan
AU  - Hadzić, V.
AU  - Todorović, V.
AU  - Lazarević Macanović, Mirjana
AU  - Petit, S. Sourice
AU  - Beck-Cormier, Sarah
AU  - Guicheux, J.
AU  - Gauthier, O.
AU  - Kovačević-Filipović, Milica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1093
AB  - The aims of this study were to define age-related histological changes in the articular cartilage of the stifle joint in non-chondrodystrophic dogs and to determine whether physical activity has a positive impact on preservation of cartilage structure during ageing. Twenty-eight German shepherd dogs were included in the study. These dogs had no evidence of joint inflammation as defined by clinical assessment, radiology and synovial fluid analysis (specifically absence of synovial fluid serum amyloid A). The dogs were grouped as young working (n = 4), young non-working (n = 5), aged working (n = 13) and aged non-working (n = 6) animals. Gross changes in the stifle joints were recorded and biopsy samples of femoral and tibial articular cartilage were evaluated for thickness; chondrocyte number, density, surface area and morphology; isogenous group morphology; tidemark integrity; subchondral bone structure; presence of proteoglycans/glycosaminoglycans; and expression of type I, II and X collagens. The major age-related changes, not related to type of physical activity, included elevated chondrocyte density and thinning of tibial cartilage and increased chondrocyte surface area in the superficial and intermediate zone of the femoral cartilage. There was also expression of type X collagen in the femoral and tibial calcified and non-calcified cartilage; however, type X collagen was not detected in the superficial zone of old working dogs. Therefore, ageing, with or without physical activity, leads to slight cartilage degeneration, while physical activity modulates the synthesis of type X collagen in the superficial cartilage zone, partially preserving the structure of hyaline cartilage.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Comparative Pathology
T1  - Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs
VL  - 151
IS  - 4
SP  - 363
EP  - 374
DO  - 10.1016/j.jcpa.2014.09.002
ER  - 

@article{
author = "Francuski, Jelena and Radovanović, Anita and Andrić, Nenad and Krstić, Vanja and Bogdanović, Dragan and Hadzić, V. and Todorović, V. and Lazarević Macanović, Mirjana and Petit, S. Sourice and Beck-Cormier, Sarah and Guicheux, J. and Gauthier, O. and Kovačević-Filipović, Milica",
year = "2014",
abstract = "The aims of this study were to define age-related histological changes in the articular cartilage of the stifle joint in non-chondrodystrophic dogs and to determine whether physical activity has a positive impact on preservation of cartilage structure during ageing. Twenty-eight German shepherd dogs were included in the study. These dogs had no evidence of joint inflammation as defined by clinical assessment, radiology and synovial fluid analysis (specifically absence of synovial fluid serum amyloid A). The dogs were grouped as young working (n = 4), young non-working (n = 5), aged working (n = 13) and aged non-working (n = 6) animals. Gross changes in the stifle joints were recorded and biopsy samples of femoral and tibial articular cartilage were evaluated for thickness; chondrocyte number, density, surface area and morphology; isogenous group morphology; tidemark integrity; subchondral bone structure; presence of proteoglycans/glycosaminoglycans; and expression of type I, II and X collagens. The major age-related changes, not related to type of physical activity, included elevated chondrocyte density and thinning of tibial cartilage and increased chondrocyte surface area in the superficial and intermediate zone of the femoral cartilage. There was also expression of type X collagen in the femoral and tibial calcified and non-calcified cartilage; however, type X collagen was not detected in the superficial zone of old working dogs. Therefore, ageing, with or without physical activity, leads to slight cartilage degeneration, while physical activity modulates the synthesis of type X collagen in the superficial cartilage zone, partially preserving the structure of hyaline cartilage.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs",
volume = "151",
number = "4",
pages = "363-374",
doi = "10.1016/j.jcpa.2014.09.002"
}

Francuski, J., Radovanović, A., Andrić, N., Krstić, V., Bogdanović, D., Hadzić, V., Todorović, V., Lazarević Macanović, M., Petit, S. S., Beck-Cormier, S., Guicheux, J., Gauthier, O.,& Kovačević-Filipović, M.. (2014). Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 151(4), 363-374.
https://doi.org/10.1016/j.jcpa.2014.09.002

Francuski J, Radovanović A, Andrić N, Krstić V, Bogdanović D, Hadzić V, Todorović V, Lazarević Macanović M, Petit SS, Beck-Cormier S, Guicheux J, Gauthier O, Kovačević-Filipović M. Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs. in Journal of Comparative Pathology. 2014;151(4):363-374.
doi:10.1016/j.jcpa.2014.09.002 .

Francuski, Jelena, Radovanović, Anita, Andrić, Nenad, Krstić, Vanja, Bogdanović, Dragan, Hadzić, V., Todorović, V., Lazarević Macanović, Mirjana, Petit, S. Sourice, Beck-Cormier, Sarah, Guicheux, J., Gauthier, O., Kovačević-Filipović, Milica, "Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs" in Journal of Comparative Pathology, 151, no. 4 (2014):363-374,
https://doi.org/10.1016/j.jcpa.2014.09.002 . .

TY  - JOUR
AU  - Nurković, Jasmin
AU  - Dolićanin, Z.
AU  - Tutić, I.
AU  - Hajrović, Š.
AU  - Mustafić, Fahrudin
AU  - Todorović, V.
AU  - Kovačević-Filipović, Milica
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1053
AB  - Stem cells are non-specialized body cells at a very early stage of development, which under normal conditions in a given tissue can differentiate into different types of functionally specialized mature cells. Mesenchymal stem cells (MSCs) are attractive candidates for clinical use in the reconstruction of damaged tissue, especially as it can be isolated from various sources and reproduced, and their use does not carry any ethical problems. Isolation methods of MSCs from adipose tissue are based on enzymatic degradation of the obtained materials. Terms for cultivation of mesenchymal stem cells are temperature of 37 °C and the partial pressure of CO2 5%. MSCs are cultured in the medium, often in a-MEM medium with 10% or 20% of fetal calf serum. Under these conditions of cultivation adherent cells form colonies in 7-14 days. MSCs are multipotent and able to differentiate in vitro conditions into Mesodermal differentiation, forming osteoblasts, chondrocytes and adipocytes. However, they can be differentiated into cells of ectodermal origin (such as neurons) and cells of endodermal origin (eg, ß-cells of the pancreatic islets of Langerhans and hepatocytes). The Laboratory for stem cell of the Department of Biomedical Sciences at the State University of Novi Pazar conducts research of MSCs originating from human adipose tissue. In collaboration with surgeons of Health Center of Novi Pazar, and respecting the norms of the Ethics Committee of both institutions in the period from 01.07.2011. to 01.07.2012. were obtained 22 samples of human subcutaneous adipose tissue of patients aged 18 to 65 years. 15 samples successfully completed the process of isolation and cultivation, and 8 induces Mesodermal differentiation.
AB  - Matične ćelije su nespecijalizovane ćelije organizma u vrlo ranom stadijumu razvića, koje u normalnim uslovima u datom tkivu mogu da se diferenciraju u različite tipove funkcionalno specijalizovanih zrelih ćelija. Mezenhimalne matične ćelije (MSCs) su atraktivni kandidati za kliničku primenu u obnavljanju oštećenih tkiva, pogotovu što se mogu izolovati iz više izvora i umnožavati, a njihova primena ne nosi nikakve etičke probleme. Metode izolacije MSCs iz masnog tkiva zasnivaju se na enzimatskom razlaganju dobijenog materijala. Uslovi kultivacije mezenhimalnih matičnih ćelija su temperatura od 37°C i parcijalni pritisak CO2 5%. MSCs se gaje u medijumu, najčešće u a-MEM medijumu sa 10% ili 20% fetalnim telećim serumom. Pod tim uslovima kultivisanja za 7-14 dana adherisane ćelije formiraju kolonije. MSCs su multipotentne i sposobne da se diferenciraju u uslovima in vitro u mezodermalnom pravcu, stvarajući osteoblaste, hondrocite i adipocite. Međutim, one mogu da se diferenciraju i u ćelije ektodermalnog (npr. neurone) i ćelije endodermalnog porekla (npr. ß-ćelije Langerhansovih ostrvaca pankreasa i hepatocite). U Laboratoriji za matične ćelije Departmana za biomedicinske nauke Državnog univerziteta u Novom Pazaru sprovodi se istraživanje MSCs poreklom iz humanog masnog tkiva. U saradnji sa hirurzima Zdravstvenog centra Novi Pazar, a uz poštovanje normi Etičkog komiteta obe ustanove u periodu od 01.07.2011. do 01.07.2012. godine dobijeno je 22 uzorka potkožnog humanog masnog tkiva od ispitanika starosti od 18 do 65 godina. Od 15 uzoraka uspešno je završen proces izolacije i kultivacije, a od 8 i ciljane mezodermalne diferencijacije.
PB  - Univerzitet u Prištini - Medicinski fakultet, Kosovska Mitrovica
T2  - Praxis medica
T1  - Adipose tissue mesenchymal stem cells: Isolation, cultivation and induced differentiation
T1  - Mezenhimalne matične ćelije iz masnog tkiva - izolacija, kultivacija i ciljana diferencijacija
VL  - 42
IS  - 3
SP  - 45
EP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1053
ER  - 

@article{
author = "Nurković, Jasmin and Dolićanin, Z. and Tutić, I. and Hajrović, Š. and Mustafić, Fahrudin and Todorović, V. and Kovačević-Filipović, Milica",
year = "2013",
abstract = "Stem cells are non-specialized body cells at a very early stage of development, which under normal conditions in a given tissue can differentiate into different types of functionally specialized mature cells. Mesenchymal stem cells (MSCs) are attractive candidates for clinical use in the reconstruction of damaged tissue, especially as it can be isolated from various sources and reproduced, and their use does not carry any ethical problems. Isolation methods of MSCs from adipose tissue are based on enzymatic degradation of the obtained materials. Terms for cultivation of mesenchymal stem cells are temperature of 37 °C and the partial pressure of CO2 5%. MSCs are cultured in the medium, often in a-MEM medium with 10% or 20% of fetal calf serum. Under these conditions of cultivation adherent cells form colonies in 7-14 days. MSCs are multipotent and able to differentiate in vitro conditions into Mesodermal differentiation, forming osteoblasts, chondrocytes and adipocytes. However, they can be differentiated into cells of ectodermal origin (such as neurons) and cells of endodermal origin (eg, ß-cells of the pancreatic islets of Langerhans and hepatocytes). The Laboratory for stem cell of the Department of Biomedical Sciences at the State University of Novi Pazar conducts research of MSCs originating from human adipose tissue. In collaboration with surgeons of Health Center of Novi Pazar, and respecting the norms of the Ethics Committee of both institutions in the period from 01.07.2011. to 01.07.2012. were obtained 22 samples of human subcutaneous adipose tissue of patients aged 18 to 65 years. 15 samples successfully completed the process of isolation and cultivation, and 8 induces Mesodermal differentiation., Matične ćelije su nespecijalizovane ćelije organizma u vrlo ranom stadijumu razvića, koje u normalnim uslovima u datom tkivu mogu da se diferenciraju u različite tipove funkcionalno specijalizovanih zrelih ćelija. Mezenhimalne matične ćelije (MSCs) su atraktivni kandidati za kliničku primenu u obnavljanju oštećenih tkiva, pogotovu što se mogu izolovati iz više izvora i umnožavati, a njihova primena ne nosi nikakve etičke probleme. Metode izolacije MSCs iz masnog tkiva zasnivaju se na enzimatskom razlaganju dobijenog materijala. Uslovi kultivacije mezenhimalnih matičnih ćelija su temperatura od 37°C i parcijalni pritisak CO2 5%. MSCs se gaje u medijumu, najčešće u a-MEM medijumu sa 10% ili 20% fetalnim telećim serumom. Pod tim uslovima kultivisanja za 7-14 dana adherisane ćelije formiraju kolonije. MSCs su multipotentne i sposobne da se diferenciraju u uslovima in vitro u mezodermalnom pravcu, stvarajući osteoblaste, hondrocite i adipocite. Međutim, one mogu da se diferenciraju i u ćelije ektodermalnog (npr. neurone) i ćelije endodermalnog porekla (npr. ß-ćelije Langerhansovih ostrvaca pankreasa i hepatocite). U Laboratoriji za matične ćelije Departmana za biomedicinske nauke Državnog univerziteta u Novom Pazaru sprovodi se istraživanje MSCs poreklom iz humanog masnog tkiva. U saradnji sa hirurzima Zdravstvenog centra Novi Pazar, a uz poštovanje normi Etičkog komiteta obe ustanove u periodu od 01.07.2011. do 01.07.2012. godine dobijeno je 22 uzorka potkožnog humanog masnog tkiva od ispitanika starosti od 18 do 65 godina. Od 15 uzoraka uspešno je završen proces izolacije i kultivacije, a od 8 i ciljane mezodermalne diferencijacije.",
publisher = "Univerzitet u Prištini - Medicinski fakultet, Kosovska Mitrovica",
journal = "Praxis medica",
title = "Adipose tissue mesenchymal stem cells: Isolation, cultivation and induced differentiation, Mezenhimalne matične ćelije iz masnog tkiva - izolacija, kultivacija i ciljana diferencijacija",
volume = "42",
number = "3",
pages = "45-50",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1053"
}

Nurković, J., Dolićanin, Z., Tutić, I., Hajrović, Š., Mustafić, F., Todorović, V.,& Kovačević-Filipović, M.. (2013). Adipose tissue mesenchymal stem cells: Isolation, cultivation and induced differentiation. in Praxis medica
Univerzitet u Prištini - Medicinski fakultet, Kosovska Mitrovica., 42(3), 45-50.
https://hdl.handle.net/21.15107/rcub_veterinar_1053

Nurković J, Dolićanin Z, Tutić I, Hajrović Š, Mustafić F, Todorović V, Kovačević-Filipović M. Adipose tissue mesenchymal stem cells: Isolation, cultivation and induced differentiation. in Praxis medica. 2013;42(3):45-50.
https://hdl.handle.net/21.15107/rcub_veterinar_1053 .

Nurković, Jasmin, Dolićanin, Z., Tutić, I., Hajrović, Š., Mustafić, Fahrudin, Todorović, V., Kovačević-Filipović, Milica, "Adipose tissue mesenchymal stem cells: Isolation, cultivation and induced differentiation" in Praxis medica, 42, no. 3 (2013):45-50,
https://hdl.handle.net/21.15107/rcub_veterinar_1053 .

TY  - JOUR
AU  - Vuković, D.
AU  - Đurković-Đaković, Olgica
AU  - Kovačević, Sanja
AU  - Bobić, Branko
AU  - Nikolíc, A.
AU  - Todorović, V.
AU  - Babić, D.
PY  - 1997
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/84
AB  - Objective: To characterize the antitoxoplasma activity of clindamycin in a murine model of acute toxoplasmosis. Methods: Rates of survival and mean survival times of Swiss Webster mice infected intraperitoneally with 106-102 tachyzoites of the RH strain of Toxoplasma gondii treated with clindamycin or sulfamethoxazole (positive control) or untreated (negative control) were compared. Survivors were submitted to examination of untreated brain tissue preparations, intraperitoneal and peroral subinoculations of brain tissue homogenates into fresh mice, and to pathohistology, including immunohistochemistry, of brain and lungs. Results: The effect of clindamycin treatment (400 mg/kg/day) on infected Swiss Webster mice was inoculum size dependent, ranging from no survivals in animals infected with 106 parasites, to 100% survivals with an inoculum of 102. Treatment initiated 24 h before and at time of infection prolonged mean survival times comparably to sulfamethoxazole, and significantly when compared to untreated controls. In contrast, treatment initiated 48 h postinfection with an inoculum of 106 did not postpone death. In the clindamycin-treated survivors, there was no biological or histologic evidence for the persistence of toxoplasma. Conclusions: The results obtained show that at an appropriate parasite dose/drug dose ratio, clindamycin is strongly toxoplasmacidal in a murine model of acute toxoplasmosis.
PB  - Blackwell Publishing Ltd
T2  - Clinical Microbiology and Infection
T1  - Effect of clindamycin in a model of acute murine toxoplasmosis
VL  - 3
IS  - 1
SP  - 89
EP  - 94
DO  - 10.1111/j.1469-0691.1997.tb00256.x
ER  - 

@article{
author = "Vuković, D. and Đurković-Đaković, Olgica and Kovačević, Sanja and Bobić, Branko and Nikolíc, A. and Todorović, V. and Babić, D.",
year = "1997",
abstract = "Objective: To characterize the antitoxoplasma activity of clindamycin in a murine model of acute toxoplasmosis. Methods: Rates of survival and mean survival times of Swiss Webster mice infected intraperitoneally with 106-102 tachyzoites of the RH strain of Toxoplasma gondii treated with clindamycin or sulfamethoxazole (positive control) or untreated (negative control) were compared. Survivors were submitted to examination of untreated brain tissue preparations, intraperitoneal and peroral subinoculations of brain tissue homogenates into fresh mice, and to pathohistology, including immunohistochemistry, of brain and lungs. Results: The effect of clindamycin treatment (400 mg/kg/day) on infected Swiss Webster mice was inoculum size dependent, ranging from no survivals in animals infected with 106 parasites, to 100% survivals with an inoculum of 102. Treatment initiated 24 h before and at time of infection prolonged mean survival times comparably to sulfamethoxazole, and significantly when compared to untreated controls. In contrast, treatment initiated 48 h postinfection with an inoculum of 106 did not postpone death. In the clindamycin-treated survivors, there was no biological or histologic evidence for the persistence of toxoplasma. Conclusions: The results obtained show that at an appropriate parasite dose/drug dose ratio, clindamycin is strongly toxoplasmacidal in a murine model of acute toxoplasmosis.",
publisher = "Blackwell Publishing Ltd",
journal = "Clinical Microbiology and Infection",
title = "Effect of clindamycin in a model of acute murine toxoplasmosis",
volume = "3",
number = "1",
pages = "89-94",
doi = "10.1111/j.1469-0691.1997.tb00256.x"
}

Vuković, D., Đurković-Đaković, O., Kovačević, S., Bobić, B., Nikolíc, A., Todorović, V.,& Babić, D.. (1997). Effect of clindamycin in a model of acute murine toxoplasmosis. in Clinical Microbiology and Infection
Blackwell Publishing Ltd., 3(1), 89-94.
https://doi.org/10.1111/j.1469-0691.1997.tb00256.x

Vuković D, Đurković-Đaković O, Kovačević S, Bobić B, Nikolíc A, Todorović V, Babić D. Effect of clindamycin in a model of acute murine toxoplasmosis. in Clinical Microbiology and Infection. 1997;3(1):89-94.
doi:10.1111/j.1469-0691.1997.tb00256.x .

Vuković, D., Đurković-Đaković, Olgica, Kovačević, Sanja, Bobić, Branko, Nikolíc, A., Todorović, V., Babić, D., "Effect of clindamycin in a model of acute murine toxoplasmosis" in Clinical Microbiology and Infection, 3, no. 1 (1997):89-94,
https://doi.org/10.1111/j.1469-0691.1997.tb00256.x . .