TY  - JOUR
AU  - Stamenić, Marko
AU  - Vulić, Jelena
AU  - Đilas, Sonja
AU  - Mišić, Dušan
AU  - Tadić, Vanja M.
AU  - Petrović, Slobodan D.
AU  - Žižović, Irena
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1115
AB  - The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE
VL  - 165
SP  - 307
EP  - 315
DO  - 10.1016/j.foodchem.2014.05.091
ER  - 

@article{
author = "Stamenić, Marko and Vulić, Jelena and Đilas, Sonja and Mišić, Dušan and Tadić, Vanja M. and Petrović, Slobodan D. and Žižović, Irena",
year = "2014",
abstract = "The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE",
volume = "165",
pages = "307-315",
doi = "10.1016/j.foodchem.2014.05.091"
}

Stamenić, M., Vulić, J., Đilas, S., Mišić, D., Tadić, V. M., Petrović, S. D.,& Žižović, I.. (2014). Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry
Elsevier Sci Ltd, Oxford., 165, 307-315.
https://doi.org/10.1016/j.foodchem.2014.05.091

Stamenić M, Vulić J, Đilas S, Mišić D, Tadić VM, Petrović SD, Žižović I. Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry. 2014;165:307-315.
doi:10.1016/j.foodchem.2014.05.091 .

Stamenić, Marko, Vulić, Jelena, Đilas, Sonja, Mišić, Dušan, Tadić, Vanja M., Petrović, Slobodan D., Žižović, Irena, "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE" in Food Chemistry, 165 (2014):307-315,
https://doi.org/10.1016/j.foodchem.2014.05.091 . .

TY  - JOUR
AU  - Tadić, Vanja M.
AU  - Bojović, Dragica
AU  - Arsić, Ivana
AU  - Đorđević, Sofija
AU  - Aksentijević, Ksenija
AU  - Stamenić, Marko
AU  - Janković, Slobodan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/879
AB  - Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
PB  - MDPI, Basel
T2  - Molecules
T1  - Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
VL  - 17
IS  - 3
SP  - 2683
EP  - 2703
DO  - 10.3390/molecules17032683
ER  - 

@article{
author = "Tadić, Vanja M. and Bojović, Dragica and Arsić, Ivana and Đorđević, Sofija and Aksentijević, Ksenija and Stamenić, Marko and Janković, Slobodan",
year = "2012",
abstract = "Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts",
volume = "17",
number = "3",
pages = "2683-2703",
doi = "10.3390/molecules17032683"
}

Tadić, V. M., Bojović, D., Arsić, I., Đorđević, S., Aksentijević, K., Stamenić, M.,& Janković, S.. (2012). Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules
MDPI, Basel., 17(3), 2683-2703.
https://doi.org/10.3390/molecules17032683

Tadić VM, Bojović D, Arsić I, Đorđević S, Aksentijević K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules. 2012;17(3):2683-2703.
doi:10.3390/molecules17032683 .

Tadić, Vanja M., Bojović, Dragica, Arsić, Ivana, Đorđević, Sofija, Aksentijević, Ksenija, Stamenić, Marko, Janković, Slobodan, "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts" in Molecules, 17, no. 3 (2012):2683-2703,
https://doi.org/10.3390/molecules17032683 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukic-Marković, Jelena
AU  - Petrović, Slobodan D.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/905
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
VL  - 72
SP  - 7
EP  - 14
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukic-Marković, Jelena and Petrović, Slobodan D.",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
volume = "72",
pages = "7-14",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukic-Marković, J.,& Petrović, S. D.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukic-Marković J, Petrović SD. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukic-Marković, Jelena, Petrović, Slobodan D., "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Stamenić, Marko
AU  - Ašanin, Ružica
AU  - Ristić, Mihailo
AU  - Petrović, Slobodan D.
AU  - Skala, Dejan
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/544
PB  - Elsevier Science Sa, Lausanne
T2  - Biochemical Engineering Journal
T1  - Antimicrobial activity of celery fruit isolates and SFE process modeling
VL  - 42
IS  - 2
SP  - 148
EP  - 152
DO  - 10.1016/j.bej.2008.06.008
ER  - 

@article{
author = "Mišić, Dušan and Žižović, Irena and Stamenić, Marko and Ašanin, Ružica and Ristić, Mihailo and Petrović, Slobodan D. and Skala, Dejan",
year = "2008",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Biochemical Engineering Journal",
title = "Antimicrobial activity of celery fruit isolates and SFE process modeling",
volume = "42",
number = "2",
pages = "148-152",
doi = "10.1016/j.bej.2008.06.008"
}

Mišić, D., Žižović, I., Stamenić, M., Ašanin, R., Ristić, M., Petrović, S. D.,& Skala, D.. (2008). Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal
Elsevier Science Sa, Lausanne., 42(2), 148-152.
https://doi.org/10.1016/j.bej.2008.06.008

Mišić D, Žižović I, Stamenić M, Ašanin R, Ristić M, Petrović SD, Skala D. Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal. 2008;42(2):148-152.
doi:10.1016/j.bej.2008.06.008 .

Mišić, Dušan, Žižović, Irena, Stamenić, Marko, Ašanin, Ružica, Ristić, Mihailo, Petrović, Slobodan D., Skala, Dejan, "Antimicrobial activity of celery fruit isolates and SFE process modeling" in Biochemical Engineering Journal, 42, no. 2 (2008):148-152,
https://doi.org/10.1016/j.bej.2008.06.008 . .

TY  - JOUR
AU  - Glisić, Sandra B.
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Žižović, Irena
AU  - Asanin, Ruzica
AU  - Skala, Dejan
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/454
AB  - Isolation of carrot fruit (Daucus carrota L., cultivar Chanteney) essential oil by supercritical carbon dioxide was investigated from the pretreatment of herbaceous matrix and extraction conditions to the chemical composition of obtained extract and its antimicrobial activity. The qualitative and quantitative analyses of the supercritical extract, as well as of the essential oil obtained by hydrodistillation, were done by GC/FID and GC/MS methods. Antimicrobial properties of both samples were investigated against ten species of microorganisms. Experimental results showed that the particle size had no influence on the extraction process. The highest yield was obtained at 40 degrees C and 10 MPa. The main component of the supercritical extract, as well as of the essential oil was carotol. The supercritical extract was characterized by the presence of heavier molecular weight compounds, while some lighter compounds, e.g. pinenes, were not detected. The supercritical extract and the essential oil were the most effective against Gram-positive bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity
VL  - 105
IS  - 1
SP  - 346
EP  - 352
DO  - 10.1016/j.foodchem.2006.11.062
ER  - 

@article{
author = "Glisić, Sandra B. and Mišić, Dušan and Stamenić, Marko and Žižović, Irena and Asanin, Ruzica and Skala, Dejan",
year = "2007",
abstract = "Isolation of carrot fruit (Daucus carrota L., cultivar Chanteney) essential oil by supercritical carbon dioxide was investigated from the pretreatment of herbaceous matrix and extraction conditions to the chemical composition of obtained extract and its antimicrobial activity. The qualitative and quantitative analyses of the supercritical extract, as well as of the essential oil obtained by hydrodistillation, were done by GC/FID and GC/MS methods. Antimicrobial properties of both samples were investigated against ten species of microorganisms. Experimental results showed that the particle size had no influence on the extraction process. The highest yield was obtained at 40 degrees C and 10 MPa. The main component of the supercritical extract, as well as of the essential oil was carotol. The supercritical extract was characterized by the presence of heavier molecular weight compounds, while some lighter compounds, e.g. pinenes, were not detected. The supercritical extract and the essential oil were the most effective against Gram-positive bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity",
volume = "105",
number = "1",
pages = "346-352",
doi = "10.1016/j.foodchem.2006.11.062"
}

Glisić, S. B., Mišić, D., Stamenić, M., Žižović, I., Asanin, R.,& Skala, D.. (2007). Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. in Food Chemistry
Elsevier Sci Ltd, Oxford., 105(1), 346-352.
https://doi.org/10.1016/j.foodchem.2006.11.062

Glisić SB, Mišić D, Stamenić M, Žižović I, Asanin R, Skala D. Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. in Food Chemistry. 2007;105(1):346-352.
doi:10.1016/j.foodchem.2006.11.062 .

Glisić, Sandra B., Mišić, Dušan, Stamenić, Marko, Žižović, Irena, Asanin, Ruzica, Skala, Dejan, "Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity" in Food Chemistry, 105, no. 1 (2007):346-352,
https://doi.org/10.1016/j.foodchem.2006.11.062 . .