TY  - THES
AU  - Aksentijević, Ksenija
PY  - 2016
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48634895
UR  - http://nardus.mpn.gov.rs/123456789/7560
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/38
AB  - During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL.
AB  - U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments
T1  - Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7560
ER  - 

@phdthesis{
author = "Aksentijević, Ksenija",
year = "2016",
abstract = "During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL., U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments, Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7560"
}

Aksentijević, K.. (2016). Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_7560

Aksentijević K. Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

Aksentijević, Ksenija, "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Genersch, Elke
AU  - Kovačević, Sanja
AU  - Ljubenković, Jovan
AU  - Radaković, Milena
AU  - Aleksić, Nevenka
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/797
AB  - Nosema species were determined in honey bees from Balkan countries. A total of 273 Nosema-positive samples were analysed. Duplex PCR and PCR-RFLP with newly designed primers, nos-16S-fw/rv, were used to differentiate between N. apis and N. ceranae. N. apis was detected in only one sample (collected in 2008 in Serbia) and N. ceranae in all the others (N = 272) including 35 older samples from Serbia collected between 2000 and 2005. No co-infection was detected. The results suggest (1) the dominance of N. ceranae infection in all Balkan countries monitored throughout the last three years; (2) the presence of N. ceranae in Serbia at least since 2000, which means that N. ceranae has not recently displaced N. apis; (3) the higher efficacy of PCR-RFLP with newly designed primers, nos-16S-fw/rv, in comparison with duplex PCR (100%: 82%, respectively). The prevalence of N. ceranae in Balkan countries was not associated with an increase in nosemosis or colony losses resembling Colony Collapse Disorder (CCD).
PB  - Springer France, Paris
T2  - Apidologie
T1  - Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder
VL  - 42
IS  - 1
SP  - 49
EP  - 58
DO  - 10.1051/apido/2010034
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Genersch, Elke and Kovačević, Sanja and Ljubenković, Jovan and Radaković, Milena and Aleksić, Nevenka",
year = "2011",
abstract = "Nosema species were determined in honey bees from Balkan countries. A total of 273 Nosema-positive samples were analysed. Duplex PCR and PCR-RFLP with newly designed primers, nos-16S-fw/rv, were used to differentiate between N. apis and N. ceranae. N. apis was detected in only one sample (collected in 2008 in Serbia) and N. ceranae in all the others (N = 272) including 35 older samples from Serbia collected between 2000 and 2005. No co-infection was detected. The results suggest (1) the dominance of N. ceranae infection in all Balkan countries monitored throughout the last three years; (2) the presence of N. ceranae in Serbia at least since 2000, which means that N. ceranae has not recently displaced N. apis; (3) the higher efficacy of PCR-RFLP with newly designed primers, nos-16S-fw/rv, in comparison with duplex PCR (100%: 82%, respectively). The prevalence of N. ceranae in Balkan countries was not associated with an increase in nosemosis or colony losses resembling Colony Collapse Disorder (CCD).",
publisher = "Springer France, Paris",
journal = "Apidologie",
title = "Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder",
volume = "42",
number = "1",
pages = "49-58",
doi = "10.1051/apido/2010034"
}

Stevanović, J., Stanimirović, Z., Genersch, E., Kovačević, S., Ljubenković, J., Radaković, M.,& Aleksić, N.. (2011). Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. in Apidologie
Springer France, Paris., 42(1), 49-58.
https://doi.org/10.1051/apido/2010034

Stevanović J, Stanimirović Z, Genersch E, Kovačević S, Ljubenković J, Radaković M, Aleksić N. Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. in Apidologie. 2011;42(1):49-58.
doi:10.1051/apido/2010034 .

Stevanović, Jevrosima, Stanimirović, Zoran, Genersch, Elke, Kovačević, Sanja, Ljubenković, Jovan, Radaković, Milena, Aleksić, Nevenka, "Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder" in Apidologie, 42, no. 1 (2011):49-58,
https://doi.org/10.1051/apido/2010034 . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Aleksić, Jelena
AU  - Fratrić, Natalija
AU  - Jakić-Dimić, Dobrila
AU  - Stojić, Velibor
AU  - Pavlović, V.
AU  - Pavlović, Miloš
AU  - Vakanjac, Slobodanka
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/675
AB  - Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu).
AB  - U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite
T1  - Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane
VL  - 60
IS  - 4
SP  - 411
EP  - 423
DO  - 10.2298/AVB1004411G
ER  - 

@article{
author = "Gvozdić, Dragan and Aleksić, Jelena and Fratrić, Natalija and Jakić-Dimić, Dobrila and Stojić, Velibor and Pavlović, V. and Pavlović, Miloš and Vakanjac, Slobodanka",
year = "2010",
abstract = "Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu)., U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite, Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane",
volume = "60",
number = "4",
pages = "411-423",
doi = "10.2298/AVB1004411G"
}

Gvozdić, D., Aleksić, J., Fratrić, N., Jakić-Dimić, D., Stojić, V., Pavlović, V., Pavlović, M.,& Vakanjac, S.. (2010). Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 411-423.
https://doi.org/10.2298/AVB1004411G

Gvozdić D, Aleksić J, Fratrić N, Jakić-Dimić D, Stojić V, Pavlović V, Pavlović M, Vakanjac S. Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd. 2010;60(4):411-423.
doi:10.2298/AVB1004411G .

Gvozdić, Dragan, Aleksić, Jelena, Fratrić, Natalija, Jakić-Dimić, Dobrila, Stojić, Velibor, Pavlović, V., Pavlović, Miloš, Vakanjac, Slobodanka, "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite" in Acta Veterinaria-Beograd, 60, no. 4 (2010):411-423,
https://doi.org/10.2298/AVB1004411G . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Dimitrijević, Vladimir
AU  - Maletić, Milan
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/717
AB  - Eleven microsatellite loci (TGLA227.BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLAS3 (14 alleles, PIC = 0.88), TGLA227(11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Animal Science
T1  - Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)
VL  - 55
IS  - 6
SP  - 221
EP  - 226
DO  - 10.17221/183/2009-CJAS
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Dimitrijević, Vladimir and Maletić, Milan",
year = "2010",
abstract = "Eleven microsatellite loci (TGLA227.BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLAS3 (14 alleles, PIC = 0.88), TGLA227(11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Animal Science",
title = "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)",
volume = "55",
number = "6",
pages = "221-226",
doi = "10.17221/183/2009-CJAS"
}

Stevanović, J., Stanimirović, Z., Dimitrijević, V.,& Maletić, M.. (2010). Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). in Czech Journal of Animal Science
Czech Academy Agricultural Sciences, Prague., 55(6), 221-226.
https://doi.org/10.17221/183/2009-CJAS

Stevanović J, Stanimirović Z, Dimitrijević V, Maletić M. Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). in Czech Journal of Animal Science. 2010;55(6):221-226.
doi:10.17221/183/2009-CJAS .

Stevanović, Jevrosima, Stanimirović, Zoran, Dimitrijević, Vladimir, Maletić, Milan, "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)" in Czech Journal of Animal Science, 55, no. 6 (2010):221-226,
https://doi.org/10.17221/183/2009-CJAS . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Radaković, Milena
AU  - Kovačević, Sanja
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/719
AB  - In this work, Apis mellifera carnica and A. m. macedonica honey bees from Serbia, Bosnia and Herzegovina and Republic of Macedonia were analysed using molecular techniques in order to improve our knowledge about biogeography of A. mellifera on the Balkan peninsula. This is the first time that the indigenous honey bees from Bosnia and Herzegovina and Republic of Macedonia have been analyzed using a molecular approach. Sampling was carried out from 560 stationary apiaries where bees were kept in traditional hives (woven skeps). The COI-COII regions of 1680 samples were PCR-amplified and sequenced. To reveal the haplotype of studied bees, the obtained sequences were aligned with published sequence data of haplotypes that belong to A. mellifera C phylogenetic lineage. The C2D mtDNA haplotype was found in all honey bees sampled from Serbia, Bosnia and Herzegovina and Republic of Macedonia, These results show that A. m. carnica and A. m. macedonica share the same C2D mtDNA haplotype. COI gene segments of 1680 samples were PCR-amplified and digested with restriction enzymes NcoI and StyI in order to discriminate A. m. macedonica from A. m. carnica. Amplified fragment patterns produced by both restriction enzymes matched with diagnostic pattern characteristic for A. m. macedonica in case of samples from east, south and south-west parts of Serbia, and Republic of Macedonia, fragments of samples from northern part of Serbia and Bosnia and Herzegovina did not include NcoI, and StyI restriction sites. These results indicate that honey bees from east, south and south-west parts of Serbia, and Republic of Macedonia belong to the A. m. macedonica, and honey bees from northern part of Serbia and Bosnia and Herzegovina belong to another subspecies, probably to the A. m. carnica. Therefore A. m. macedonica has much wider area of distribution than it was previously considered.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Genetics
T1  - Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses
VL  - 46
IS  - 5
SP  - 603
EP  - 609
DO  - 10.1134/S1022795410050145
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Radaković, Milena and Kovačević, Sanja",
year = "2010",
abstract = "In this work, Apis mellifera carnica and A. m. macedonica honey bees from Serbia, Bosnia and Herzegovina and Republic of Macedonia were analysed using molecular techniques in order to improve our knowledge about biogeography of A. mellifera on the Balkan peninsula. This is the first time that the indigenous honey bees from Bosnia and Herzegovina and Republic of Macedonia have been analyzed using a molecular approach. Sampling was carried out from 560 stationary apiaries where bees were kept in traditional hives (woven skeps). The COI-COII regions of 1680 samples were PCR-amplified and sequenced. To reveal the haplotype of studied bees, the obtained sequences were aligned with published sequence data of haplotypes that belong to A. mellifera C phylogenetic lineage. The C2D mtDNA haplotype was found in all honey bees sampled from Serbia, Bosnia and Herzegovina and Republic of Macedonia, These results show that A. m. carnica and A. m. macedonica share the same C2D mtDNA haplotype. COI gene segments of 1680 samples were PCR-amplified and digested with restriction enzymes NcoI and StyI in order to discriminate A. m. macedonica from A. m. carnica. Amplified fragment patterns produced by both restriction enzymes matched with diagnostic pattern characteristic for A. m. macedonica in case of samples from east, south and south-west parts of Serbia, and Republic of Macedonia, fragments of samples from northern part of Serbia and Bosnia and Herzegovina did not include NcoI, and StyI restriction sites. These results indicate that honey bees from east, south and south-west parts of Serbia, and Republic of Macedonia belong to the A. m. macedonica, and honey bees from northern part of Serbia and Bosnia and Herzegovina belong to another subspecies, probably to the A. m. carnica. Therefore A. m. macedonica has much wider area of distribution than it was previously considered.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Genetics",
title = "Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses",
volume = "46",
number = "5",
pages = "603-609",
doi = "10.1134/S1022795410050145"
}

Stevanović, J., Stanimirović, Z., Radaković, M.,& Kovačević, S.. (2010). Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses. in Russian Journal of Genetics
Maik Nauka/Interperiodica/Springer, New York., 46(5), 603-609.
https://doi.org/10.1134/S1022795410050145

Stevanović J, Stanimirović Z, Radaković M, Kovačević S. Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses. in Russian Journal of Genetics. 2010;46(5):603-609.
doi:10.1134/S1022795410050145 .

Stevanović, Jevrosima, Stanimirović, Zoran, Radaković, Milena, Kovačević, Sanja, "Biogeographic Study of the Honey Bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on Mitochondrial DNA Analyses" in Russian Journal of Genetics, 46, no. 5 (2010):603-609,
https://doi.org/10.1134/S1022795410050145 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Milićević, Zorka
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/676
AB  - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.
AB  - Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro
T1  - Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro
VL  - 62
IS  - 1
SP  - 63
EP  - 74
DO  - 10.2298/ABS1001063B
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Milićević, Zorka and Živković, Lada and Spremo-Potparević, Biljana",
year = "2010",
abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment., Prevremena centromerna deoba (PCD) se može posmatrati kao manifestacija hromozomske nestabilnosti. U cilju procene efekta paklitaksela (Ptx) i cikloheksimida (Cy) na indukciju PCD-a, koristili smo mikronukleus test (CBMN) uz fluorescentnu in situ hibridizaciju (FISH), kao i test hromozomskih aberacija (CA) na humanim limfocitima periferne krvi. Rezultati su pokazali da Ptx sam, ili u kombinaciji sa Cy može da indukuje PCD. Ovi nalazi ukazuju da treba mnogo više pažnje obratiti na pojavu PCD-a kao parametra genotoksičnosti u prekliničkim ispitivanjima mono- i/ili politerapija za lečenje kancera.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro, Efekat samog paklitaksela, i u kombinaciji sa cikloheksimidom, na učestalost prevremene centromerne deobe in vitro",
volume = "62",
number = "1",
pages = "63-74",
doi = "10.2298/ABS1001063B"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Milićević, Z., Živković, L.,& Spremo-Potparević, B.. (2010). The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 63-74.
https://doi.org/10.2298/ABS1001063B

Bajić V, Stanimirović Z, Stevanović J, Milićević Z, Živković L, Spremo-Potparević B. The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro. in Archives of Biological Sciences. 2010;62(1):63-74.
doi:10.2298/ABS1001063B .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Milićević, Zorka, Živković, Lada, Spremo-Potparević, Biljana, "The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74,
https://doi.org/10.2298/ABS1001063B . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Aleksić, Nevenka
AU  - Stojić, Velibor
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/683
AB  - Grooming behavior is considered an important defensive mechanism of honey bees against Varroa mites. The aim of this study was to reveal whether grooming behavior is a useful criterion in breeding of Varroa-tolerant bees. To obtain a reliable evaluation the environmental influences were excluded. The degree of grooming potential was estimated by the percentage of damaged mites in the total number of fallen mites. The heritability of grooming behavior throughout the three consecutive generations of queens was assessed by mother-daughter regression method. Among unselected queens, expressed grooming behavior was recorded only in colonies with F1 queens (36.27%), but not in colonies with P queens and F2 queens (33.69%, 31.66%, respectively). Significant differences in grooming behavior were found between colonies of P and F1 queens (p lt 0.001), and between colonies of P and F2 queens (p lt 0.05). However, all of the three generations of selected queens showed expressed grooming behavior (37.99%, 39.42% and 38.58% in Ps, F1s and F2s, respectively) without significant (p>0.05) difference among them. Nevertheless, the relatively low heritability of grooming behavior in the three generations of queens examined (h2yx=0.49±0.02; h2zx=0.18±0.01; h2zy=0.16±0.01) indicate that breeding colonies for grooming behavior only cannot be advised to beekeepers whose aim is to breed bees highly tolerant to Varroa mites.
AB  - Negovateljsko ponašanje se smatra značajnim mehanizmom odbrane pčela od Varroa krpelja. Cilj ovog rada je bilo ispitivanje negovateljskog ponašanja, procena njegove heritabilnosti i mogućnosti povećanja ekspresije te osobine putem selekcije. Radi dobijanja pouzdanih rezultata korišćenja je metodologija kojom se uticaj spoljašnjih faktora isključuje. Ispoljenost negovateljskog ponašanja procenjivana je na osnovu procenta oštećenih u ukupnom broju otpalih krpelja. Heritabilnost negovateljskog ponašanja praćena na kroz generacije matica i procenjivana metodom regresije majka-ćerka. Među neselekcionisanim maticama, negovateljsko ponašanje bilo je izraženo samo kod matica F1 generacije (36,27%), ali ne i kod P (33,69%) i F2 generacije (31,66%). Statistički značajne razlike u negovateljskom ponašanju zabeležene su između društava P i F1 matica (p lt 0,001) i između društava P i F2 matica (p lt 0,05). Međutim, selekcionisane matice sve tri generacije (Ps, F1s, F2s) su imale izraženo negovateljsko ponašanje (37,99%, 39,42% i 38,58%) bez statistički značajnih (p>0,05) razlika među njima. Ipak, nizak koeficijent heritabilnosti praćene osobine (h2yx=0,49±0,02; h2zx=0,18±0,01; h2zy=0,16±0,01) ukazuje da se pčelarima ne može preporučiti selekcija pčela samo na negovateljsko ponašanje ako je njihov cilj uzgoj pčelinjih zajednica povećane otpornosti na Varroa krpelje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica)
T1  - Heritabilnost negovateljskog ponašanja sive medonosne pčele (Apis mellifera Carnica)
VL  - 60
IS  - 2-3
SP  - 313
EP  - 323
DO  - 10.2298/AVB1003313S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Aleksić, Nevenka and Stojić, Velibor",
year = "2010",
abstract = "Grooming behavior is considered an important defensive mechanism of honey bees against Varroa mites. The aim of this study was to reveal whether grooming behavior is a useful criterion in breeding of Varroa-tolerant bees. To obtain a reliable evaluation the environmental influences were excluded. The degree of grooming potential was estimated by the percentage of damaged mites in the total number of fallen mites. The heritability of grooming behavior throughout the three consecutive generations of queens was assessed by mother-daughter regression method. Among unselected queens, expressed grooming behavior was recorded only in colonies with F1 queens (36.27%), but not in colonies with P queens and F2 queens (33.69%, 31.66%, respectively). Significant differences in grooming behavior were found between colonies of P and F1 queens (p lt 0.001), and between colonies of P and F2 queens (p lt 0.05). However, all of the three generations of selected queens showed expressed grooming behavior (37.99%, 39.42% and 38.58% in Ps, F1s and F2s, respectively) without significant (p>0.05) difference among them. Nevertheless, the relatively low heritability of grooming behavior in the three generations of queens examined (h2yx=0.49±0.02; h2zx=0.18±0.01; h2zy=0.16±0.01) indicate that breeding colonies for grooming behavior only cannot be advised to beekeepers whose aim is to breed bees highly tolerant to Varroa mites., Negovateljsko ponašanje se smatra značajnim mehanizmom odbrane pčela od Varroa krpelja. Cilj ovog rada je bilo ispitivanje negovateljskog ponašanja, procena njegove heritabilnosti i mogućnosti povećanja ekspresije te osobine putem selekcije. Radi dobijanja pouzdanih rezultata korišćenja je metodologija kojom se uticaj spoljašnjih faktora isključuje. Ispoljenost negovateljskog ponašanja procenjivana je na osnovu procenta oštećenih u ukupnom broju otpalih krpelja. Heritabilnost negovateljskog ponašanja praćena na kroz generacije matica i procenjivana metodom regresije majka-ćerka. Među neselekcionisanim maticama, negovateljsko ponašanje bilo je izraženo samo kod matica F1 generacije (36,27%), ali ne i kod P (33,69%) i F2 generacije (31,66%). Statistički značajne razlike u negovateljskom ponašanju zabeležene su između društava P i F1 matica (p lt 0,001) i između društava P i F2 matica (p lt 0,05). Međutim, selekcionisane matice sve tri generacije (Ps, F1s, F2s) su imale izraženo negovateljsko ponašanje (37,99%, 39,42% i 38,58%) bez statistički značajnih (p>0,05) razlika među njima. Ipak, nizak koeficijent heritabilnosti praćene osobine (h2yx=0,49±0,02; h2zx=0,18±0,01; h2zy=0,16±0,01) ukazuje da se pčelarima ne može preporučiti selekcija pčela samo na negovateljsko ponašanje ako je njihov cilj uzgoj pčelinjih zajednica povećane otpornosti na Varroa krpelje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica), Heritabilnost negovateljskog ponašanja sive medonosne pčele (Apis mellifera Carnica)",
volume = "60",
number = "2-3",
pages = "313-323",
doi = "10.2298/AVB1003313S"
}

Stanimirović, Z., Stevanović, J., Aleksić, N.,& Stojić, V.. (2010). Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 313-323.
https://doi.org/10.2298/AVB1003313S

Stanimirović Z, Stevanović J, Aleksić N, Stojić V. Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica). in Acta Veterinaria-Beograd. 2010;60(2-3):313-323.
doi:10.2298/AVB1003313S .

Stanimirović, Zoran, Stevanović, Jevrosima, Aleksić, Nevenka, Stojić, Velibor, "Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica)" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):313-323,
https://doi.org/10.2298/AVB1003313S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Pušić, M.
AU  - Jakić-Dimić, Dobrila
AU  - Vujanac, Ivan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/655
AB  - The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05).
AB  - Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving
T1  - Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja
VL  - 59
IS  - 1
SP  - 17
EP  - 23
DO  - 10.2298/AVB0901017S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Pušić, M. and Jakić-Dimić, Dobrila and Vujanac, Ivan",
year = "2009",
abstract = "The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05)., Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving, Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja",
volume = "59",
number = "1",
pages = "17-23",
doi = "10.2298/AVB0901017S"
}

Šamanc, H., Stojić, V., Kirovski, D., Pušić, M., Jakić-Dimić, D.,& Vujanac, I.. (2009). Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 17-23.
https://doi.org/10.2298/AVB0901017S

Šamanc H, Stojić V, Kirovski D, Pušić M, Jakić-Dimić D, Vujanac I. Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd. 2009;59(1):17-23.
doi:10.2298/AVB0901017S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Pušić, M., Jakić-Dimić, Dobrila, Vujanac, Ivan, "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving" in Acta Veterinaria-Beograd, 59, no. 1 (2009):17-23,
https://doi.org/10.2298/AVB0901017S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Pudlo, Pavle
AU  - Vujanac, Ivan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/632
AB  - The aim of this study was to examine the secretory capacity of endocrine pancreas beta cells in healthy cows and cows suffering from left displaced abomasum (LDA) by determination of glucose and insulin concentrations in the peripheral circulation during glucose tolerance test (GTT). A total of twenty healthy cows (Control group) and twenty cows suffering from left abomasal displacement (Experimental group) were chosen for this study. Cows in the control group were exposed to GTT once, while cows in the experimental group were exposed on the day, day 3 and day 6 after the diagnosis of LDA, as well as 3 days after surgical treatment and reposition of the abomasum. Initial blood glucose concentrations in healthy cows were significantly lower than in the cows suffering from LDA only on day 3 (P lt 0.05) and day 6 (P lt 0.01) after the disease was diagnosed. All the glucose values determined both in healthy and diseased cows at 30. minutes after the start of glucose infusion were much higher than physiological values. Regardless to the decline of glucose levels from 60 to 180 minutes after start of glucose infusion, hyperglicemia maintained in both healthy cows and cows suffering from LDA. Compared to healthy cows the rate of decline was slower in cows suffering from LDA at the day of diagnosis, as well as 3 and 6 days after the diagnosis. At the end of the experiment, 240 minutes after the start of the infusion, glucose concentrations were significantly higher in diseased cows on the day of diagnosis and day 3 and 6 after diagnosis than in the control group (P lt 0.001, respectively) and in diseased cows 3 days after surgery (P lt 0.001, respectively). Mean initial concentrations of serum insulin were significantly higher in healthy cows than in cows suffering from LDA only on day 6 after diagnosis. From 30 to 240 minutes after the start of glucose infusion, insulinemic responses to intravenous glucose administation were statistically higher in healthy cows than in experimental cows on the day of diagnosis, day 3 and day 6 after diagnosis, as well as 3 days after surgery. On the day of diagnosis and three days after, insulinemia decreased to initial levels 180 minutes after the start of the infusion. Six days after diagnosis, insulinemia decreased to initial values at 120 minutes after the start of the glucose infusion. Three days after surgery, insulinemia decrased to values similar to initial at 240 minutes after the start of the infusion. Glucose tolerance test results showed that the best validation of pancreas endocrine function was between 60 and 120 minutes, as well as 180 and 240 minutes after the glucose infusion started. Our results also confirmed that cows suffering from LDA are in the stage of pancreatic endocrine disfunction and that the rate of this disfunction was higher when the disease lasted longer. The highest rate of pancreatic endocrine disfunction was observed 6 days after diagnosis. On day 3 after surgical treatment and reposition of the abomasum, glucose tolerance test results showed that the pancreatic endocrine function recovered.
AB  - Cilj ovog rada bio je da se, određivanjem koncentracije glukoze i insulina u krvi krava tokom testa opterećenja glukozom, ispita sekretorni kapacitet beta ćelija endokrinog pankreasa kod zdravih krava i krava obolelih od dislokacije sirišta na levo. U ogled je uključeno 20 zdravih krava (kontrolna grupa) i 20 krava obolelih od dislokacije sirišta na levo (eksperimentalna grupa). Kod zdravih krava opterećenje glukozom je izvršeno jednom, dok je kod obolelih krava ovaj test izveden na dan dijagnostikovanja oboljenja, 3 i 6 dana posle, kao i 3 dana nakon hirurške intervencije i repozicije obolelog organa. Bazalna koncentracija glukoze, određena neposredno pre početka infuzije, je kod zdravih krava bila značajno niža nego kod krava obolelih od dislokacije sirišta na levo tri dana (P lt 0,05) i šest dana (P lt 0,01) nakon dijagnostikovanja bolesti. Tridesetog minuta nakon početka infuzije, hiperglikemija je utvrđena i kod zdravih i kod bolesnih krava. Iako je koncentracija glukoze i kod zdravih i kod bolesnih krava opadala od 60. do 180. minuta posle početka infuzije, hiperglikemija se kod svih krava održala tokom ovog perioda. U poređenju sa zdravim kravama, stepen opadanja koncentracije glukoze je bio sporiji kod bolesnih krava na dan dijagnostikovanja dislokacije sirišta na levo, kao i tri do šest dana posle. Na kraju eksperimenta, 240 minuta nakon početka infuzije, koncentracija glukoze je bila statistički značajno viša kod obolelih krava na dan dijagnostikovanja bolesti, kao i tri i šest dana kasnije u odnosu na vrednost određenu u to vreme kod kontrolne grupe krava (P lt 0,001, pojedinačno) i obolelih krava tri dana nakon repozicije abomasuma (P lt 0,001, pojedinačno). Bazalna vrednost insulinemije, određena neposredno pre početka infuzije glukoze, je bila značajno viša kod zdravih u odnosu na krave obolele od dislokacije sirišta šestog dana nakon postavljanja dijagnoze (P lt 0,001). U periodu od 30. do 240. minuta nakon početka infuzije glukoze, insulinski odgovor na aplikovanu glukozu je bio značajno viši kod zdravih krava u odnosu na bolesne krave na dan dijagnostikovanja dislokacije, tri i šest dana posle kao i 3 dana nakon hirurške repozicije abomasuma. Na dan dijagnostikovanja bolesti kao i tri dana kasnije, insulinemija je opala na vrednosti slične bazalnim 180. minuta nakon početka infuzije glukoze. Šest dana nakon dijagnostikovanja bolesti, koncentracija insulina je opala na vrednosti slične bazalnim 120 minuta nakon početka infuzije. Tri dana nakon repozicije abomasuma, insulinemija je smanjena na vrednosti slične bazalnim 240. minuta nakon početka infuzije. Rezultati testa opterećenja glukozom daju najbolju procenu endokrine funkcije pankreasa između 60. i 120. minuta i 120. i 180. minuta posle početka infuzije glukoze. Naši rezultati ukazuju da kod krava obolelih od dislokacije sirišta na levo postoji stanje insuficijencije endokrinog pankreasa i da se to stanje pogoršava sa dužim tokom bolesti. Najveći stepen insuficijencije je ustanovljen šesti dan od dijagnostikovanja oboljenja. Tri dana nakon izvođenja hirurške intervencije i repozicije dislociranog organa, rezultati testa opterećenja glukozom pokazuju da se ponovo uspostavlja normalna aktivnost endokrinog pankreasa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum
T1  - Primena testa opterećenja glukozom u oceni endokrine funkcije pankreasa kod krava obolelih od dislokacije sirišta na levo pre i posle hirurškog tretmana
VL  - 59
IS  - 5-6
SP  - 513
EP  - 523
DO  - 10.2298/AVB0906513S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Pudlo, Pavle and Vujanac, Ivan",
year = "2009",
abstract = "The aim of this study was to examine the secretory capacity of endocrine pancreas beta cells in healthy cows and cows suffering from left displaced abomasum (LDA) by determination of glucose and insulin concentrations in the peripheral circulation during glucose tolerance test (GTT). A total of twenty healthy cows (Control group) and twenty cows suffering from left abomasal displacement (Experimental group) were chosen for this study. Cows in the control group were exposed to GTT once, while cows in the experimental group were exposed on the day, day 3 and day 6 after the diagnosis of LDA, as well as 3 days after surgical treatment and reposition of the abomasum. Initial blood glucose concentrations in healthy cows were significantly lower than in the cows suffering from LDA only on day 3 (P lt 0.05) and day 6 (P lt 0.01) after the disease was diagnosed. All the glucose values determined both in healthy and diseased cows at 30. minutes after the start of glucose infusion were much higher than physiological values. Regardless to the decline of glucose levels from 60 to 180 minutes after start of glucose infusion, hyperglicemia maintained in both healthy cows and cows suffering from LDA. Compared to healthy cows the rate of decline was slower in cows suffering from LDA at the day of diagnosis, as well as 3 and 6 days after the diagnosis. At the end of the experiment, 240 minutes after the start of the infusion, glucose concentrations were significantly higher in diseased cows on the day of diagnosis and day 3 and 6 after diagnosis than in the control group (P lt 0.001, respectively) and in diseased cows 3 days after surgery (P lt 0.001, respectively). Mean initial concentrations of serum insulin were significantly higher in healthy cows than in cows suffering from LDA only on day 6 after diagnosis. From 30 to 240 minutes after the start of glucose infusion, insulinemic responses to intravenous glucose administation were statistically higher in healthy cows than in experimental cows on the day of diagnosis, day 3 and day 6 after diagnosis, as well as 3 days after surgery. On the day of diagnosis and three days after, insulinemia decreased to initial levels 180 minutes after the start of the infusion. Six days after diagnosis, insulinemia decreased to initial values at 120 minutes after the start of the glucose infusion. Three days after surgery, insulinemia decrased to values similar to initial at 240 minutes after the start of the infusion. Glucose tolerance test results showed that the best validation of pancreas endocrine function was between 60 and 120 minutes, as well as 180 and 240 minutes after the glucose infusion started. Our results also confirmed that cows suffering from LDA are in the stage of pancreatic endocrine disfunction and that the rate of this disfunction was higher when the disease lasted longer. The highest rate of pancreatic endocrine disfunction was observed 6 days after diagnosis. On day 3 after surgical treatment and reposition of the abomasum, glucose tolerance test results showed that the pancreatic endocrine function recovered., Cilj ovog rada bio je da se, određivanjem koncentracije glukoze i insulina u krvi krava tokom testa opterećenja glukozom, ispita sekretorni kapacitet beta ćelija endokrinog pankreasa kod zdravih krava i krava obolelih od dislokacije sirišta na levo. U ogled je uključeno 20 zdravih krava (kontrolna grupa) i 20 krava obolelih od dislokacije sirišta na levo (eksperimentalna grupa). Kod zdravih krava opterećenje glukozom je izvršeno jednom, dok je kod obolelih krava ovaj test izveden na dan dijagnostikovanja oboljenja, 3 i 6 dana posle, kao i 3 dana nakon hirurške intervencije i repozicije obolelog organa. Bazalna koncentracija glukoze, određena neposredno pre početka infuzije, je kod zdravih krava bila značajno niža nego kod krava obolelih od dislokacije sirišta na levo tri dana (P lt 0,05) i šest dana (P lt 0,01) nakon dijagnostikovanja bolesti. Tridesetog minuta nakon početka infuzije, hiperglikemija je utvrđena i kod zdravih i kod bolesnih krava. Iako je koncentracija glukoze i kod zdravih i kod bolesnih krava opadala od 60. do 180. minuta posle početka infuzije, hiperglikemija se kod svih krava održala tokom ovog perioda. U poređenju sa zdravim kravama, stepen opadanja koncentracije glukoze je bio sporiji kod bolesnih krava na dan dijagnostikovanja dislokacije sirišta na levo, kao i tri do šest dana posle. Na kraju eksperimenta, 240 minuta nakon početka infuzije, koncentracija glukoze je bila statistički značajno viša kod obolelih krava na dan dijagnostikovanja bolesti, kao i tri i šest dana kasnije u odnosu na vrednost određenu u to vreme kod kontrolne grupe krava (P lt 0,001, pojedinačno) i obolelih krava tri dana nakon repozicije abomasuma (P lt 0,001, pojedinačno). Bazalna vrednost insulinemije, određena neposredno pre početka infuzije glukoze, je bila značajno viša kod zdravih u odnosu na krave obolele od dislokacije sirišta šestog dana nakon postavljanja dijagnoze (P lt 0,001). U periodu od 30. do 240. minuta nakon početka infuzije glukoze, insulinski odgovor na aplikovanu glukozu je bio značajno viši kod zdravih krava u odnosu na bolesne krave na dan dijagnostikovanja dislokacije, tri i šest dana posle kao i 3 dana nakon hirurške repozicije abomasuma. Na dan dijagnostikovanja bolesti kao i tri dana kasnije, insulinemija je opala na vrednosti slične bazalnim 180. minuta nakon početka infuzije glukoze. Šest dana nakon dijagnostikovanja bolesti, koncentracija insulina je opala na vrednosti slične bazalnim 120 minuta nakon početka infuzije. Tri dana nakon repozicije abomasuma, insulinemija je smanjena na vrednosti slične bazalnim 240. minuta nakon početka infuzije. Rezultati testa opterećenja glukozom daju najbolju procenu endokrine funkcije pankreasa između 60. i 120. minuta i 120. i 180. minuta posle početka infuzije glukoze. Naši rezultati ukazuju da kod krava obolelih od dislokacije sirišta na levo postoji stanje insuficijencije endokrinog pankreasa i da se to stanje pogoršava sa dužim tokom bolesti. Najveći stepen insuficijencije je ustanovljen šesti dan od dijagnostikovanja oboljenja. Tri dana nakon izvođenja hirurške intervencije i repozicije dislociranog organa, rezultati testa opterećenja glukozom pokazuju da se ponovo uspostavlja normalna aktivnost endokrinog pankreasa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum, Primena testa opterećenja glukozom u oceni endokrine funkcije pankreasa kod krava obolelih od dislokacije sirišta na levo pre i posle hirurškog tretmana",
volume = "59",
number = "5-6",
pages = "513-523",
doi = "10.2298/AVB0906513S"
}

Šamanc, H., Stojić, V., Kirovski, D., Pudlo, P.,& Vujanac, I.. (2009). Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 513-523.
https://doi.org/10.2298/AVB0906513S

Šamanc H, Stojić V, Kirovski D, Pudlo P, Vujanac I. Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum. in Acta Veterinaria-Beograd. 2009;59(5-6):513-523.
doi:10.2298/AVB0906513S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Pudlo, Pavle, Vujanac, Ivan, "Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):513-523,
https://doi.org/10.2298/AVB0906513S . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Spremo-Potparević, Biljana
AU  - Zivković, L.
AU  - Milicević, Z.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/614
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_614
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Spremo-Potparević, Biljana and Zivković, L. and Milicević, Z.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_614"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Spremo-Potparević, B., Zivković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_veterinar_614

Bajić V, Stanimirović Z, Stevanović J, Spremo-Potparević B, Zivković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_veterinar_614 .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Spremo-Potparević, Biljana, Zivković, L., Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_veterinar_614 .

TY  - JOUR
AU  - Jovanović, Milan
AU  - Lazarević, Miodrag
AU  - Popović, N.
AU  - Vojvodić, Danilo
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/613
AB  - Immunomodulation is a phenomenon that is based on the influence of different endogenous and exogenous substances on the function of molecules and cells pertaining to the immune system. Some components extracted from the bovine thymus have immunomodulating features and can, both in animals and humans, by suppressing the immune response (humoral and/or cellular) bring about immunostimulation and increased organism resistance. In this paper the effects of bovine thymus extract (Thymex - L) on the parameters of humoral immune response (concentration of antibodies to tetanus toxoid - ATTx) and cellular immune response (interleukin 2 - IL-2, interferon γ - IFN-γ and degree of cutaneus basophyl hypersensitivity - CBHR) in rats previously irradiated by X-rays at a dose of 4 or 6 Gy. The studied thymus extract had shown positive effects on IL-2 and IFN-γ serum concentrations in irradiated rats. In the group of rats irradiated with 4 Gy the effects were evident on the 7th day of the trial. Rats irradiated with the higher dose (6 Gy) experienced the positive effects of thymus extract on the 21st day of the experiment. In the skin PHA hypersensitivity test, a positive effect was also recorded, being more pronounced in rats which received the lower irradiation dose. The tested thymus extract had shown a positive effect on the concentration of ATTx as early as the 14th day of the trial.
AB  - Pojedini sastojci ekstrakta goveđeg timusa imaju imunomodulatorna svojstva i mogu, kod ljudi i životinja, sa suprimiranim funkcijama imunskog sistema (humoralnim i/ili celularnim) dovesti do imunostimulacije. U ovom radu su prikazani rezultati ispitivanja efekata preparata Thymex-L dobijenog iz ekstrakta goveđ eg timusa na parametre humoralnog imunskog odgovora pacova kod kojih je prethodno izvršeno jednokratno ozračivanje X zracima u dozi od 4 ili 6 Gy. Određivani su sledeći parametri: koncentracija interleukina 2 (IL-2), interferona γ (IFN-γ), koncentracija antitela posle vakcinacije i revakcinacije tetanusnim toksoidom (ATTx) i stepen kožne preosetljivosti (CBHR) na biljni mitogen (PHA) Ispitivani ekstrakt je ispoljio pozitivan efekat na koncentraciju IL-2 i IFN-γ u serumu ozračenih pacova. Ovaj pozitivan efekat je, kod grupe pacova koja je primila nižu dozu zračenja, vidljiv već nakon 7. dana eksperimenta, dok je kod pacova ozračenih višom dozom zračenja pozitivan efekat registrovan 21. dana eksperimenta. Pri proceni stepena reakcije kožne preosetljivosti na PHA, registrovan je pozitivan efekat primenjenog ekstrakta timusa i on je bio izraženiji kod jedinki kod kojih je bila primenjena niža doza zračenja. Ispitivani ekstrakt goveđeg timusa je ispoljio pozitivan efekat i na koncentraciju ATTx i to već 14. dana eksperimenta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The influence of bovine thymic extract on the immune response in irradiated wistar rats
T1  - Efekat ekstrakta goveđeg timusa na imunski odgovor ozračenih wistar pacova
VL  - 59
IS  - 4
SP  - 331
EP  - 347
DO  - 10.2298/AVB0904331J
ER  - 

@article{
author = "Jovanović, Milan and Lazarević, Miodrag and Popović, N. and Vojvodić, Danilo",
year = "2009",
abstract = "Immunomodulation is a phenomenon that is based on the influence of different endogenous and exogenous substances on the function of molecules and cells pertaining to the immune system. Some components extracted from the bovine thymus have immunomodulating features and can, both in animals and humans, by suppressing the immune response (humoral and/or cellular) bring about immunostimulation and increased organism resistance. In this paper the effects of bovine thymus extract (Thymex - L) on the parameters of humoral immune response (concentration of antibodies to tetanus toxoid - ATTx) and cellular immune response (interleukin 2 - IL-2, interferon γ - IFN-γ and degree of cutaneus basophyl hypersensitivity - CBHR) in rats previously irradiated by X-rays at a dose of 4 or 6 Gy. The studied thymus extract had shown positive effects on IL-2 and IFN-γ serum concentrations in irradiated rats. In the group of rats irradiated with 4 Gy the effects were evident on the 7th day of the trial. Rats irradiated with the higher dose (6 Gy) experienced the positive effects of thymus extract on the 21st day of the experiment. In the skin PHA hypersensitivity test, a positive effect was also recorded, being more pronounced in rats which received the lower irradiation dose. The tested thymus extract had shown a positive effect on the concentration of ATTx as early as the 14th day of the trial., Pojedini sastojci ekstrakta goveđeg timusa imaju imunomodulatorna svojstva i mogu, kod ljudi i životinja, sa suprimiranim funkcijama imunskog sistema (humoralnim i/ili celularnim) dovesti do imunostimulacije. U ovom radu su prikazani rezultati ispitivanja efekata preparata Thymex-L dobijenog iz ekstrakta goveđ eg timusa na parametre humoralnog imunskog odgovora pacova kod kojih je prethodno izvršeno jednokratno ozračivanje X zracima u dozi od 4 ili 6 Gy. Određivani su sledeći parametri: koncentracija interleukina 2 (IL-2), interferona γ (IFN-γ), koncentracija antitela posle vakcinacije i revakcinacije tetanusnim toksoidom (ATTx) i stepen kožne preosetljivosti (CBHR) na biljni mitogen (PHA) Ispitivani ekstrakt je ispoljio pozitivan efekat na koncentraciju IL-2 i IFN-γ u serumu ozračenih pacova. Ovaj pozitivan efekat je, kod grupe pacova koja je primila nižu dozu zračenja, vidljiv već nakon 7. dana eksperimenta, dok je kod pacova ozračenih višom dozom zračenja pozitivan efekat registrovan 21. dana eksperimenta. Pri proceni stepena reakcije kožne preosetljivosti na PHA, registrovan je pozitivan efekat primenjenog ekstrakta timusa i on je bio izraženiji kod jedinki kod kojih je bila primenjena niža doza zračenja. Ispitivani ekstrakt goveđeg timusa je ispoljio pozitivan efekat i na koncentraciju ATTx i to već 14. dana eksperimenta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The influence of bovine thymic extract on the immune response in irradiated wistar rats, Efekat ekstrakta goveđeg timusa na imunski odgovor ozračenih wistar pacova",
volume = "59",
number = "4",
pages = "331-347",
doi = "10.2298/AVB0904331J"
}

Jovanović, M., Lazarević, M., Popović, N.,& Vojvodić, D.. (2009). The influence of bovine thymic extract on the immune response in irradiated wistar rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 331-347.
https://doi.org/10.2298/AVB0904331J

Jovanović M, Lazarević M, Popović N, Vojvodić D. The influence of bovine thymic extract on the immune response in irradiated wistar rats. in Acta Veterinaria-Beograd. 2009;59(4):331-347.
doi:10.2298/AVB0904331J .

Jovanović, Milan, Lazarević, Miodrag, Popović, N., Vojvodić, Danilo, "The influence of bovine thymic extract on the immune response in irradiated wistar rats" in Acta Veterinaria-Beograd, 59, no. 4 (2009):331-347,
https://doi.org/10.2298/AVB0904331J . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Stojić, Velibor
AU  - Šamanc, Horea
AU  - Fratrić, Natalija
AU  - Daković, Aleksandra
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/563
AB  - Sixty Holstein newborn calves, which were immediately after parturition separated from their dams and placed in individual pens, were randomly assigned to 4 treatment groups (15 calves per group). All calves were bottle-fed twice a day during the first 48 hours after partus, with their mothers' colostrum in 12 hour intervals, starting two hours after partus, according to the following model: i) group 1 (G1) received 0.75 L of colostrum (C) per meal, with 20 mL of 25% zeolite (clinoptilolite) suspension in distilled water; ii) group 2 (G2) received 1.5L of C per meal, with 20 mL of 25% zeolite suspension in distilled water; iii) group 3 (G3) received 0.75 L of C per meal, and iv) group 4 (G4) received 1.5 L of C per meal. Blood serum immunoglobulin G (IgG) concentrations at 0h (before C ingestion) 6h, 24h and 48h after partus and colostrum IgG concentrations were determined using commercial sRID plates (INEP, Zemun, Serbia). Apparent efficiency of absorption (AEA%) was calculated for individual calves at 6h after partus. Calf blood serum IgG concentrations at all investigated time intervals in G1 were increased compared to G3, with a significant increase at 6h after partus (15±4:10±5 g/L, p lt 0.05). The same relationship was evident between G2 and G4, with significant mean IgG blood serum differences evident at 6, 24. and 48. hour after partus. Calf blood serum IgG concentration was significantly higher in G2 compared to all experimental groups at 6, 24 and 48h after partus. Apparent efficiency of absorption (AEA%) at 6h after partus was significantly higher in G1, compared to all other experimental groups.
AB  - Šezdeset novorođenih teladi Holštajn rase, koja su neposredno nakon rođenja odvojena od majki i stavljena u individualne bokseve, su metodom slučajnog izbora podeljene u 4 ogledne grupe (15 teladi po grupi). Sva telad su za vreme prvih 48 sati života hranjena dva puta dnevno (u intervalu od 12 sati) kolostrumom majki, počevši od drugog sata nakon partusa prema sledećem eksperimentalnom modelu: i) grupa 1 (G1) dobijala je 0.75 L kolostruma (C) po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; ii) grupa 2 (G2) dobijala je 1.5 L C po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; iii) grupa 3 (G3) dobijala je 0.75L C po obroku; iv) grupa 4 (G4) dobijala je 1.5 L C po obroku. Koncentracija imunoglobulina G (IgG) određivana je komercijalnim radijalnim imunodifuzionim testom (sRID, INEP, Zemin) u krvnom serumu dobijenom iz uzoraka krvi uzetih punkcijom v. jugularis pre uzimanja kolosruma (0. sat), 6-og, 24-og i 48-og sata nakon rođenja, dok je relativna efikasnost apsorpcije imunoglobulina (AEA%) određena 6-og sata po rođenju. Koncentracija IgG u krvnom serumu kod teladi iz grupe G1 u odnosu na G2 bila je viša 6-og, 24-og i 48-og sata, dok je statistička značajnost razlika srednjih vrednosti ustanovljena 6-og sata po rođenju (15±4:10±5 g/L, p lt 0.05). Isti odnos srednjih vrednosti koncentracije IgG u krvnom serumu novorođenih teladi je zabeležen između grupe G2 i G4, gde su statistički značajno više srednje vrednosti utvrđene kod grupe G2 u odnosu na G4 u periodu 6, 24. i 48. sati po rođenju (p lt 0.01 za sve navedene vremenske periode). Relativna efikasnost apsorpcije 6-og sata po rođenju je bila statistički vrlo značajno viša (p lt 0.01) kod grupe G1 u odnosu na ostale ogledne grupe teladi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite
T1  - Relativna efikasnost resorpcije imunoglobulina kod novorođene teladi oralno tretirane zeolitom
VL  - 58
IS  - 4
SP  - 345
EP  - 355
DO  - 10.2298/AVB0804345G
ER  - 

@article{
author = "Gvozdić, Dragan and Stojić, Velibor and Šamanc, Horea and Fratrić, Natalija and Daković, Aleksandra",
year = "2008",
abstract = "Sixty Holstein newborn calves, which were immediately after parturition separated from their dams and placed in individual pens, were randomly assigned to 4 treatment groups (15 calves per group). All calves were bottle-fed twice a day during the first 48 hours after partus, with their mothers' colostrum in 12 hour intervals, starting two hours after partus, according to the following model: i) group 1 (G1) received 0.75 L of colostrum (C) per meal, with 20 mL of 25% zeolite (clinoptilolite) suspension in distilled water; ii) group 2 (G2) received 1.5L of C per meal, with 20 mL of 25% zeolite suspension in distilled water; iii) group 3 (G3) received 0.75 L of C per meal, and iv) group 4 (G4) received 1.5 L of C per meal. Blood serum immunoglobulin G (IgG) concentrations at 0h (before C ingestion) 6h, 24h and 48h after partus and colostrum IgG concentrations were determined using commercial sRID plates (INEP, Zemun, Serbia). Apparent efficiency of absorption (AEA%) was calculated for individual calves at 6h after partus. Calf blood serum IgG concentrations at all investigated time intervals in G1 were increased compared to G3, with a significant increase at 6h after partus (15±4:10±5 g/L, p lt 0.05). The same relationship was evident between G2 and G4, with significant mean IgG blood serum differences evident at 6, 24. and 48. hour after partus. Calf blood serum IgG concentration was significantly higher in G2 compared to all experimental groups at 6, 24 and 48h after partus. Apparent efficiency of absorption (AEA%) at 6h after partus was significantly higher in G1, compared to all other experimental groups., Šezdeset novorođenih teladi Holštajn rase, koja su neposredno nakon rođenja odvojena od majki i stavljena u individualne bokseve, su metodom slučajnog izbora podeljene u 4 ogledne grupe (15 teladi po grupi). Sva telad su za vreme prvih 48 sati života hranjena dva puta dnevno (u intervalu od 12 sati) kolostrumom majki, počevši od drugog sata nakon partusa prema sledećem eksperimentalnom modelu: i) grupa 1 (G1) dobijala je 0.75 L kolostruma (C) po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; ii) grupa 2 (G2) dobijala je 1.5 L C po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; iii) grupa 3 (G3) dobijala je 0.75L C po obroku; iv) grupa 4 (G4) dobijala je 1.5 L C po obroku. Koncentracija imunoglobulina G (IgG) određivana je komercijalnim radijalnim imunodifuzionim testom (sRID, INEP, Zemin) u krvnom serumu dobijenom iz uzoraka krvi uzetih punkcijom v. jugularis pre uzimanja kolosruma (0. sat), 6-og, 24-og i 48-og sata nakon rođenja, dok je relativna efikasnost apsorpcije imunoglobulina (AEA%) određena 6-og sata po rođenju. Koncentracija IgG u krvnom serumu kod teladi iz grupe G1 u odnosu na G2 bila je viša 6-og, 24-og i 48-og sata, dok je statistička značajnost razlika srednjih vrednosti ustanovljena 6-og sata po rođenju (15±4:10±5 g/L, p lt 0.05). Isti odnos srednjih vrednosti koncentracije IgG u krvnom serumu novorođenih teladi je zabeležen između grupe G2 i G4, gde su statistički značajno više srednje vrednosti utvrđene kod grupe G2 u odnosu na G4 u periodu 6, 24. i 48. sati po rođenju (p lt 0.01 za sve navedene vremenske periode). Relativna efikasnost apsorpcije 6-og sata po rođenju je bila statistički vrlo značajno viša (p lt 0.01) kod grupe G1 u odnosu na ostale ogledne grupe teladi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite, Relativna efikasnost resorpcije imunoglobulina kod novorođene teladi oralno tretirane zeolitom",
volume = "58",
number = "4",
pages = "345-355",
doi = "10.2298/AVB0804345G"
}

Gvozdić, D., Stojić, V., Šamanc, H., Fratrić, N.,& Daković, A.. (2008). Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(4), 345-355.
https://doi.org/10.2298/AVB0804345G

Gvozdić D, Stojić V, Šamanc H, Fratrić N, Daković A. Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite. in Acta Veterinaria-Beograd. 2008;58(4):345-355.
doi:10.2298/AVB0804345G .

Gvozdić, Dragan, Stojić, Velibor, Šamanc, Horea, Fratrić, Natalija, Daković, Aleksandra, "Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite" in Acta Veterinaria-Beograd, 58, no. 4 (2008):345-355,
https://doi.org/10.2298/AVB0804345G . .

TY  - JOUR
AU  - Milanović, Svetlana
AU  - Lazarević, Miodrag
AU  - Jokić, Živan
AU  - Jovanović, Ivan B.
AU  - Pešut, Olivera
AU  - Kirovski, Danijela
AU  - Marinković, Darko
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/552
AB  - The aim of this study was to investigate the influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens. The trial was conducted on 200 'Arbor Acres' chickens randomly alloted in four equal groups. Birds from all groups were fed standard broiler feed, supplemented with 40 mg/kg of Fe originating from different sources: Group I (FeSO4), Group II (Fe bounded to yeast), Group III (ferrous ascorbate) and Group IV (iron chelate). From each group, 10 birds were sacrificed on 21st, 35th and 42nd day and the following parameters were measured: erythrocyte count, hematocrite value, hemoglobin concentration, concentration of nonheme iron in spleen, liver and bone marrow (femur), degree of cutaneous hypersensitivity to PHA and titers of antibodies to Gumboro virus following vaccination. Addition of organic iron supplements resulted in increased erythrocyte count, hemoglobin concentration and hematocrite value on the 21st day. Different iron forms did not change the concentration of nonheme iron in the liver on the 21st and 42nd day. On the 35th day, the group supplemented with ferrous ascorbate had lower liver iron concentration. Also, concentrations of nonheme iron in the spleen were lower in groups supplemented with organic iron forms. The concentration of iron in the bone marrow decreased with age and the lowest values were recorded in the ferrous ascorbate supplemented group. The degree of cutaneous hypersensitivity to PHA was higher in groups supplemented with organic iron forms on the 21st and 35th day. Titers of anti-Gumboro antibodies were higher in the group supplemented with iron helate on the 35th day, but later (day 42) no significant differences were observed among groups.
AB  - Cilj ovog rada je bio da se ispita uticaj organski i neorganski vezanog gvožđa na crvenu krvnu sliku, imunski odgovor i količinu gvožđa u pojedinim organima. Ogled je izveden na ukupno 200 brojlera podeljenih u četiri jednake grupe. U smeše za ishranu brojlera dodavano je gvožđe u količini od 40 mg/kg koje je poticalo iz različitih izvora: fero sulfat (ogledna grupa I), gvožđe vezano za kvasac (ogledna grupa II), fero askorbat (ogledna grupa III) i helatno gvožđe (ogledna grupa IV). Kod piladi u dobi od 21, 35 i 42 dana praćeni su sledeći parametri: broj eritrocita, koncentracija hemoglobina, količina nehemskog gvožđa u jetri, slezini i kostnoj srži, vrednosti testa kožne preosetljivosti na fitohemaglutinin (PHA) i titar specifičnih antitela u krvnoj plazmi posle vakcinacije protiv Gumboro bolesti. Kod piladi u dobi od 21 dan svi ispitivani preparati gvožđa u kojima je ono bilo organski vezano, doveli su do povećanja broja eritrocita i koncentracije hemoglobina. Različiti oblici gvožđa u ishrani živine nisu uticali na količinu depononovanog nehemskog gvožđa u jetri piladi u dobi od 21 i 42 dan. Pilad u dobi od 35 dana, koja su u hrani dobijala fero askorbat, imala su značajno manje gvožđa deponovanog u jetri. Količina nehemskog gvožđa u slezini bila je manja kod piladi koja su hranom dobijala organski vezano gvožđe. Količina nehemskog gvožđa u kostnoj srži butne kosti piladi smanjivala se sa starošću jedinki a najmanja količina nehemskog gvožđa u kostnoj srži je zabeležena u grupi koja je hranom dobijala fero askorbat. Stepen kožne reaktivnosti na PHA bio je veći kod jedinki u dobi od 21 i 35 dana suplementiranih organski vezanim gvožđem. Upotreba helatno vezanog gvožđa u ishrani brojlera dovela je do statistički značajno veće produkcije antitela protiv Gumboro virusa nakon vakcinacije, kod piladi u dobi od 35 dana. Titar antitela kod jedinki u dobi od 42 dana u svim oglednim grupama bio je ujednačen.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens
T1  - Uticaj organski i neorganski vezanog gvožđa na hematološke parametre, imunski odgovor i količinu gvožđa u organima brojlera
VL  - 58
IS  - 2-3
SP  - 179
EP  - 189
DO  - 10.2298/AVB0803179M
ER  - 

@article{
author = "Milanović, Svetlana and Lazarević, Miodrag and Jokić, Živan and Jovanović, Ivan B. and Pešut, Olivera and Kirovski, Danijela and Marinković, Darko",
year = "2008",
abstract = "The aim of this study was to investigate the influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens. The trial was conducted on 200 'Arbor Acres' chickens randomly alloted in four equal groups. Birds from all groups were fed standard broiler feed, supplemented with 40 mg/kg of Fe originating from different sources: Group I (FeSO4), Group II (Fe bounded to yeast), Group III (ferrous ascorbate) and Group IV (iron chelate). From each group, 10 birds were sacrificed on 21st, 35th and 42nd day and the following parameters were measured: erythrocyte count, hematocrite value, hemoglobin concentration, concentration of nonheme iron in spleen, liver and bone marrow (femur), degree of cutaneous hypersensitivity to PHA and titers of antibodies to Gumboro virus following vaccination. Addition of organic iron supplements resulted in increased erythrocyte count, hemoglobin concentration and hematocrite value on the 21st day. Different iron forms did not change the concentration of nonheme iron in the liver on the 21st and 42nd day. On the 35th day, the group supplemented with ferrous ascorbate had lower liver iron concentration. Also, concentrations of nonheme iron in the spleen were lower in groups supplemented with organic iron forms. The concentration of iron in the bone marrow decreased with age and the lowest values were recorded in the ferrous ascorbate supplemented group. The degree of cutaneous hypersensitivity to PHA was higher in groups supplemented with organic iron forms on the 21st and 35th day. Titers of anti-Gumboro antibodies were higher in the group supplemented with iron helate on the 35th day, but later (day 42) no significant differences were observed among groups., Cilj ovog rada je bio da se ispita uticaj organski i neorganski vezanog gvožđa na crvenu krvnu sliku, imunski odgovor i količinu gvožđa u pojedinim organima. Ogled je izveden na ukupno 200 brojlera podeljenih u četiri jednake grupe. U smeše za ishranu brojlera dodavano je gvožđe u količini od 40 mg/kg koje je poticalo iz različitih izvora: fero sulfat (ogledna grupa I), gvožđe vezano za kvasac (ogledna grupa II), fero askorbat (ogledna grupa III) i helatno gvožđe (ogledna grupa IV). Kod piladi u dobi od 21, 35 i 42 dana praćeni su sledeći parametri: broj eritrocita, koncentracija hemoglobina, količina nehemskog gvožđa u jetri, slezini i kostnoj srži, vrednosti testa kožne preosetljivosti na fitohemaglutinin (PHA) i titar specifičnih antitela u krvnoj plazmi posle vakcinacije protiv Gumboro bolesti. Kod piladi u dobi od 21 dan svi ispitivani preparati gvožđa u kojima je ono bilo organski vezano, doveli su do povećanja broja eritrocita i koncentracije hemoglobina. Različiti oblici gvožđa u ishrani živine nisu uticali na količinu depononovanog nehemskog gvožđa u jetri piladi u dobi od 21 i 42 dan. Pilad u dobi od 35 dana, koja su u hrani dobijala fero askorbat, imala su značajno manje gvožđa deponovanog u jetri. Količina nehemskog gvožđa u slezini bila je manja kod piladi koja su hranom dobijala organski vezano gvožđe. Količina nehemskog gvožđa u kostnoj srži butne kosti piladi smanjivala se sa starošću jedinki a najmanja količina nehemskog gvožđa u kostnoj srži je zabeležena u grupi koja je hranom dobijala fero askorbat. Stepen kožne reaktivnosti na PHA bio je veći kod jedinki u dobi od 21 i 35 dana suplementiranih organski vezanim gvožđem. Upotreba helatno vezanog gvožđa u ishrani brojlera dovela je do statistički značajno veće produkcije antitela protiv Gumboro virusa nakon vakcinacije, kod piladi u dobi od 35 dana. Titar antitela kod jedinki u dobi od 42 dana u svim oglednim grupama bio je ujednačen.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens, Uticaj organski i neorganski vezanog gvožđa na hematološke parametre, imunski odgovor i količinu gvožđa u organima brojlera",
volume = "58",
number = "2-3",
pages = "179-189",
doi = "10.2298/AVB0803179M"
}

Milanović, S., Lazarević, M., Jokić, Ž., Jovanović, I. B., Pešut, O., Kirovski, D.,& Marinković, D.. (2008). The influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(2-3), 179-189.
https://doi.org/10.2298/AVB0803179M

Milanović S, Lazarević M, Jokić Ž, Jovanović IB, Pešut O, Kirovski D, Marinković D. The influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens. in Acta Veterinaria-Beograd. 2008;58(2-3):179-189.
doi:10.2298/AVB0803179M .

Milanović, Svetlana, Lazarević, Miodrag, Jokić, Živan, Jovanović, Ivan B., Pešut, Olivera, Kirovski, Danijela, Marinković, Darko, "The influence of organic and inorganic Fe supplementation on red blood picture, immune response and quantity of iron in organs of broiler chickens" in Acta Veterinaria-Beograd, 58, no. 2-3 (2008):179-189,
https://doi.org/10.2298/AVB0803179M . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Mirilović, Milorad
AU  - Stojić, Velibor
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/551
AB  - Breeding grey A. m. carnica queens for hygienic behavior is beneficial for commercial beekeeping in Serbia, since hygienic behavior is associated with the resistance to the mite Varroa destructor, as well as to American foulbrood and chalk brood. In this work, heritability of hygienic behavior was analyzed in autochthonous grey A. m. carnica honey bees from Šumadija region in order to ascertain if the expression of their hygienic behavior could be enhanced through selective breeding. Heritability of hygienic behavior was monitored through three queen generations and estimated by one-parent-offspring regression method (motherdaughter regression method). The heritability values for hygienic behavior were h2=0.63±0.02 in relationship between daughter queens (F1s) and breeder mother queens (Ps), h2=0.45±0.01 in relationship between grand-daughter queens (F2s) and Ps and h2=0.44±0.02 in relationship between F2s and F1s. These results show that the expression of hygienic behavior through selective breeding could be enhanced and the best results could be achieved in the F1 generation. It is important to emphasize that the F1 colonies bred for hygienic behavior in our study suffer no apparent costs in reproductive and productive performances.
AB  - Odgajanje matica sive medonosne pčele A. m. carnica sa ispoljenim higijenskim ponašanjem od velikog je značaja za komercijalno pčelarenje u Srbiji, obzirom da je higijensko ponašanje povezano sa otpornošću prema krpelju Varroa destructor, kao i prema izazivačima bolesti američke truleži pčelinjeg legla i krečnog legla. U ovom radu, ispitivana je heritabilnost higijenskog ponašanja kod autohtone sive medonosne pčele A. m. carnica iz Šumadije da bi se utvrdilo da li ispoljenost higijenskog ponašanja kod tih pčela može da se poveća putem selektivnog gajenja. Heritabilnost higijenskog ponašanja praćena je kroz tri generacije matica i procenjivana metodom regresije jedan roditelj-potomak (metoda regresije majka-ćerka). Vrednosti koeficijenta heritabilnosti higijenskog ponašanja bile su h2=0,63±0,02 između ćerki matica (F1s) i majki matica (Ps), h2=0,45±0,01 između unuka matica (F2s) i Ps, i h2=0.44±0.02 između F2s i F1s. Ovi rezultati pokazuju da ispoljenost higijenskog ponašanja kod analiziranih pčela može biti povećana, kao i da se najbolji rezultati mogu postići u F1 generaciji. Pri tome je značajno istaći da kod društava F1 generacije odgajenih na higijensko ponašanje u našem radu nije bilo nikakvih negativnih posledica u pogledu reproduktivnih i produktivnih sposobnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica)
T1  - Heritabilnost higijenskog ponašanja sive medonosne pčele (Apis mellifera carnica)
VL  - 58
IS  - 5-6
SP  - 593
EP  - 601
DO  - 10.2298/AVB0806593S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Mirilović, Milorad and Stojić, Velibor",
year = "2008",
abstract = "Breeding grey A. m. carnica queens for hygienic behavior is beneficial for commercial beekeeping in Serbia, since hygienic behavior is associated with the resistance to the mite Varroa destructor, as well as to American foulbrood and chalk brood. In this work, heritability of hygienic behavior was analyzed in autochthonous grey A. m. carnica honey bees from Šumadija region in order to ascertain if the expression of their hygienic behavior could be enhanced through selective breeding. Heritability of hygienic behavior was monitored through three queen generations and estimated by one-parent-offspring regression method (motherdaughter regression method). The heritability values for hygienic behavior were h2=0.63±0.02 in relationship between daughter queens (F1s) and breeder mother queens (Ps), h2=0.45±0.01 in relationship between grand-daughter queens (F2s) and Ps and h2=0.44±0.02 in relationship between F2s and F1s. These results show that the expression of hygienic behavior through selective breeding could be enhanced and the best results could be achieved in the F1 generation. It is important to emphasize that the F1 colonies bred for hygienic behavior in our study suffer no apparent costs in reproductive and productive performances., Odgajanje matica sive medonosne pčele A. m. carnica sa ispoljenim higijenskim ponašanjem od velikog je značaja za komercijalno pčelarenje u Srbiji, obzirom da je higijensko ponašanje povezano sa otpornošću prema krpelju Varroa destructor, kao i prema izazivačima bolesti američke truleži pčelinjeg legla i krečnog legla. U ovom radu, ispitivana je heritabilnost higijenskog ponašanja kod autohtone sive medonosne pčele A. m. carnica iz Šumadije da bi se utvrdilo da li ispoljenost higijenskog ponašanja kod tih pčela može da se poveća putem selektivnog gajenja. Heritabilnost higijenskog ponašanja praćena je kroz tri generacije matica i procenjivana metodom regresije jedan roditelj-potomak (metoda regresije majka-ćerka). Vrednosti koeficijenta heritabilnosti higijenskog ponašanja bile su h2=0,63±0,02 između ćerki matica (F1s) i majki matica (Ps), h2=0,45±0,01 između unuka matica (F2s) i Ps, i h2=0.44±0.02 između F2s i F1s. Ovi rezultati pokazuju da ispoljenost higijenskog ponašanja kod analiziranih pčela može biti povećana, kao i da se najbolji rezultati mogu postići u F1 generaciji. Pri tome je značajno istaći da kod društava F1 generacije odgajenih na higijensko ponašanje u našem radu nije bilo nikakvih negativnih posledica u pogledu reproduktivnih i produktivnih sposobnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica), Heritabilnost higijenskog ponašanja sive medonosne pčele (Apis mellifera carnica)",
volume = "58",
number = "5-6",
pages = "593-601",
doi = "10.2298/AVB0806593S"
}

Stanimirović, Z., Stevanović, J., Mirilović, M.,& Stojić, V.. (2008). Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 593-601.
https://doi.org/10.2298/AVB0806593S

Stanimirović Z, Stevanović J, Mirilović M, Stojić V. Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica). in Acta Veterinaria-Beograd. 2008;58(5-6):593-601.
doi:10.2298/AVB0806593S .

Stanimirović, Zoran, Stevanović, Jevrosima, Mirilović, Milorad, Stojić, Velibor, "Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica)" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):593-601,
https://doi.org/10.2298/AVB0806593S . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Radaković, Milena
AU  - Stojić, Velibor
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/545
AB  - Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.
PB  - Wiley, Hoboken
T2  - Environmental and Molecular Mutagenesis
T1  - In Vitro Evaluation of the Clastogenicity of Fumagillin
VL  - 49
IS  - 8
SP  - 594
EP  - 601
DO  - 10.1002/em.20409
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Radaković, Milena and Stojić, Velibor",
year = "2008",
abstract = "Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.",
publisher = "Wiley, Hoboken",
journal = "Environmental and Molecular Mutagenesis",
title = "In Vitro Evaluation of the Clastogenicity of Fumagillin",
volume = "49",
number = "8",
pages = "594-601",
doi = "10.1002/em.20409"
}

Stevanović, J., Stanimirović, Z., Radaković, M.,& Stojić, V.. (2008). In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis
Wiley, Hoboken., 49(8), 594-601.
https://doi.org/10.1002/em.20409

Stevanović J, Stanimirović Z, Radaković M, Stojić V. In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis. 2008;49(8):594-601.
doi:10.1002/em.20409 .

Stevanović, Jevrosima, Stanimirović, Zoran, Radaković, Milena, Stojić, Velibor, "In Vitro Evaluation of the Clastogenicity of Fumagillin" in Environmental and Molecular Mutagenesis, 49, no. 8 (2008):594-601,
https://doi.org/10.1002/em.20409 . .

TY  - JOUR
AU  - Dakić, Zorica
AU  - Kulišić, Zoran
AU  - Stajković, Novica
AU  - Pelemiš, M.
AU  - Čobeljić, M.
AU  - Stanimirović, Zoran
AU  - Inđić, N.
AU  - Poluga, Jasmina
AU  - Pavlović, M.
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/554
AB  - Belgrade is situated in the area that is potentially at risk from malaria outbrakes. Until eradication, the main vector of malaria in this area was Anopheles maculipennis s. s. (previous name An. typicus) and secondary vectors were An. messeae and An. atroparvus. In this study we examined the distribution and ecology of Anopheles mosquitoes (Diptera, Culicidae) in Belgrade. Females of Anopheles mosquitoes were collected from animal shelters in Belgrade at eight locations during 2003. Egg morphology was used to identify the specimens. A total of 3704 females deposited eggs ready for identification. Three species of An. maculipennis complex were identified: An. messeae, An. atroparvus and An. maculipennis s. s.. The most abundant species were An. messeae (64%). The relative frequency of three species varied depending on the site of collection. Seasonal fluctuations of mosquitoes' species varied. Each develops in a distinct type of water, too. The three species of the An. maculipennis complex, particularly An. messeae and An. atroparvus, are considered as potential vectors of malaria in Belgrade. With the possible reintroduction of Plasmodium species due to climatic changes and increased travel to and from the countries where malaria is endemic, a more efficient vector control is necessary.
AB  - Beograd je smešten u području koje je potencijalno rizično za ponovno uspostavljanje transmisije malarije. Do eradikacije malarije, glavni vektor malarije na području Beograda bio je An. maculipennis s. s. (raniji naziv An. typicus) a sekundarni vektori bili su An. messeae i An. atroparvus. Mi smo analizirali distribuciju i ekologiju komaraca roda Anopheles (Diptera, Culicidae) na području Beograda. Ženke komaraca roda Anopheles sakupljali smo tokom 2003. godine na 8 lokaliteta šireg područja Beograda. Identifikaciju vrsta vršili smo na osnovu morfologije položenih jaja. Od ukupnog broja izlovljenih ženki komaraca roda Anopheles, njih 3704 je položilo jaja, a njihovom identifikacijom nađene su tri vrste komaraca roda Anopheles, svi pripadnici Anopheles maculipennis kompleksa: An. messeae, An. atroparvus i An. maculipennis s. s.. U ukupnoj populaciji najzastupljeniji je bio An. messeae 64%, zatim An. atroparvus 21%, a najmanje Anopheles maculipennis s. s. 8%. Postojala je razlika u procentualnoj zastupljenosti ovih vrsta u ukupnoj Anopheles populaciji prema lokalitetima, po mesecima, prema izboru vodenih staništa. Prisutne vrste Anopheles komaraca, posebno An. messeae i An. atroparvus ukazuju da je Beograd receptivan za transmisiju malarije, a klimatski uslovi tokom leta pogodni su za kompletiranje sporogoničnog razvoja pripadnika roda Plasmodium.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ecology of Anopheles mosquitoes in Belgrade area: Estimating vector potential for malaria retransmission
T1  - Ekologija komaraca roda Anopheles na području Beograda u proceni vektorskog potencijala za ponovno uspostavljanje transmisije malarije
VL  - 58
IS  - 5-6
SP  - 603
EP  - 614
DO  - 10.2298/AVB0806603D
ER  - 

@article{
author = "Dakić, Zorica and Kulišić, Zoran and Stajković, Novica and Pelemiš, M. and Čobeljić, M. and Stanimirović, Zoran and Inđić, N. and Poluga, Jasmina and Pavlović, M.",
year = "2008",
abstract = "Belgrade is situated in the area that is potentially at risk from malaria outbrakes. Until eradication, the main vector of malaria in this area was Anopheles maculipennis s. s. (previous name An. typicus) and secondary vectors were An. messeae and An. atroparvus. In this study we examined the distribution and ecology of Anopheles mosquitoes (Diptera, Culicidae) in Belgrade. Females of Anopheles mosquitoes were collected from animal shelters in Belgrade at eight locations during 2003. Egg morphology was used to identify the specimens. A total of 3704 females deposited eggs ready for identification. Three species of An. maculipennis complex were identified: An. messeae, An. atroparvus and An. maculipennis s. s.. The most abundant species were An. messeae (64%). The relative frequency of three species varied depending on the site of collection. Seasonal fluctuations of mosquitoes' species varied. Each develops in a distinct type of water, too. The three species of the An. maculipennis complex, particularly An. messeae and An. atroparvus, are considered as potential vectors of malaria in Belgrade. With the possible reintroduction of Plasmodium species due to climatic changes and increased travel to and from the countries where malaria is endemic, a more efficient vector control is necessary., Beograd je smešten u području koje je potencijalno rizično za ponovno uspostavljanje transmisije malarije. Do eradikacije malarije, glavni vektor malarije na području Beograda bio je An. maculipennis s. s. (raniji naziv An. typicus) a sekundarni vektori bili su An. messeae i An. atroparvus. Mi smo analizirali distribuciju i ekologiju komaraca roda Anopheles (Diptera, Culicidae) na području Beograda. Ženke komaraca roda Anopheles sakupljali smo tokom 2003. godine na 8 lokaliteta šireg područja Beograda. Identifikaciju vrsta vršili smo na osnovu morfologije položenih jaja. Od ukupnog broja izlovljenih ženki komaraca roda Anopheles, njih 3704 je položilo jaja, a njihovom identifikacijom nađene su tri vrste komaraca roda Anopheles, svi pripadnici Anopheles maculipennis kompleksa: An. messeae, An. atroparvus i An. maculipennis s. s.. U ukupnoj populaciji najzastupljeniji je bio An. messeae 64%, zatim An. atroparvus 21%, a najmanje Anopheles maculipennis s. s. 8%. Postojala je razlika u procentualnoj zastupljenosti ovih vrsta u ukupnoj Anopheles populaciji prema lokalitetima, po mesecima, prema izboru vodenih staništa. Prisutne vrste Anopheles komaraca, posebno An. messeae i An. atroparvus ukazuju da je Beograd receptivan za transmisiju malarije, a klimatski uslovi tokom leta pogodni su za kompletiranje sporogoničnog razvoja pripadnika roda Plasmodium.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ecology of Anopheles mosquitoes in Belgrade area: Estimating vector potential for malaria retransmission, Ekologija komaraca roda Anopheles na području Beograda u proceni vektorskog potencijala za ponovno uspostavljanje transmisije malarije",
volume = "58",
number = "5-6",
pages = "603-614",
doi = "10.2298/AVB0806603D"
}

Dakić, Z., Kulišić, Z., Stajković, N., Pelemiš, M., Čobeljić, M., Stanimirović, Z., Inđić, N., Poluga, J.,& Pavlović, M.. (2008). Ecology of Anopheles mosquitoes in Belgrade area: Estimating vector potential for malaria retransmission. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 603-614.
https://doi.org/10.2298/AVB0806603D

Dakić Z, Kulišić Z, Stajković N, Pelemiš M, Čobeljić M, Stanimirović Z, Inđić N, Poluga J, Pavlović M. Ecology of Anopheles mosquitoes in Belgrade area: Estimating vector potential for malaria retransmission. in Acta Veterinaria-Beograd. 2008;58(5-6):603-614.
doi:10.2298/AVB0806603D .

Dakić, Zorica, Kulišić, Zoran, Stajković, Novica, Pelemiš, M., Čobeljić, M., Stanimirović, Zoran, Inđić, N., Poluga, Jasmina, Pavlović, M., "Ecology of Anopheles mosquitoes in Belgrade area: Estimating vector potential for malaria retransmission" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):603-614,
https://doi.org/10.2298/AVB0806603D . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Stojić, Velibor
AU  - Rajčić, S.
AU  - Radojičić, Biljana
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/472
AB  - The aim of the study was to investigate the influence of added natural mineral adsorbent zeolite (clinoptylolite) on the degree of absorption of immunoglobulin G and on glucose and protein concentration in the blood of calves during the neonatal period. Sixty calves were randomly divided in to four groups (15 calves per group). The quality and timing of colostrum feeding was as follows: group I was fed only with 1.5 L of colostrum at 2nd, 12th, 24th and 36th hour after birth; group II was fed with 1.5 L of colostrum with the mineral adsorbent added at a 5% concentration in the same time intervals; group III was fed with 1.5 L of colostrum together with the mineral adsorbent added at a 5% concentration, but at 2nd and 12 hour after birth it was fed with the first colostrum, and 24 and 36 hours after birth it was fed with the second colostrum. Group IV of calves was fed the same way as the third one only without the addition of the mineral adsorbent. Mean serum concentration of IgG was 15.14±7.43; 22.22±8.15; 23.65±9.93; 20.81±4.19 g/L in groups I to IV respectively at 6h of age. IgG concentration in the sera of all groups were 29.10±15.25; 45.46±13.45; 50.72±21.57; 41.60±15.42 g/L respectively at 16h after birth. At 30 hours of age, concentration of IgG was 33.29±14.70; 47.05±12.38; 55.00±19.71; 51.59±13.82 g/L in groups I to IV respectively. IgG concentration in the sera of all groups were 29.82±12.69; 44.10±13.50; 51.88±20.14; 47.68±13.18 g/L respectively at 40 hours after birth. For the calves which were fed with the full colostrum without and with the addition of the mineral adsorbent (Group I and II) the statistical significance of the difference in the concentration of IgG was determined during all the tested intervals. Concentration of IgG in the sera of group II calves (fed colostrum with zeolite added) was approximately 30% higher than in the group I. The statistical significance of the difference in levels of IgG between group I and groups III an IV was determined during all tested intervals, also. Concentration of IgG in the sera of group III calves was 40% higher than in group I. Acta Veterinaria (Beograd), Vol. 57, No. 2-3, 169-180, 2007. Results suggest that calves should receive feedings of high quality colostrum with added mineral adsorbent in order to maximize colostral IgG absorption. Determining the proteinemia in the neonatal period of calves is a reliable indicator of the impact of the mineral adsorbent on the degree of absorption of colostral IgG. The concentration of glucose for all the tested groups increased significantly with the increase of the quantity of ingested colostrum, with no evident influence of the added mineral adsorbent.
AB  - Cilj ovog rada bio je da se ispita uticaj mineralnog adsorbenta na bazi klinoptilolita na stepen resorpcije IgG i koncentraciju nekih biohemijskih parametara u krvnom serumu teladi u neonatalnom periodu. Telad su bila podeljena u četiri grupe (po 15 teladi u svakoj grupi). I grupa teladi napajana je sa kolostrumom 2h, 12h, 24h i 36h posle rođenja, II grupa teladi napajana je sa kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% u istim vremenskim intervalima, III grupa napajana je sa 1,5 L kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% ali 2h i 12h posle rođenja napajana su prvim kolostrumom, a 24h i 36h dugim kolostrumom, IV grupa teladi hranjena je na isti način kao i III grupa samo što u kolostrum nije bio dodat mineralni adsorbent. Koncentracija IgG u krvnom serumu teladi od I do IV grupe, 6 sata iznosila je pojedinačno 15,14±7,43; 22,22±8,15; 23,65±9,93; 20,81±4,19 g/L. U krvnom serumu teladi kod svih ispitivanih grupa koncentracija IgG u 16h iznosila je pojedinač no 29,10±15,25; 45,46±13,45; 50,72±21,57; 41,60±15,42 g/L. Koncentracija IgG 30sata kod ispitivanih grupa teladi (I do IV) bila je pojedinačno 33,29±14,70; 47,05±12,38; 55,00±19,71; 51,59±13,82 g/L. Koncentracija IgG u krvnom serumu svih grupa 40h iznosila je 29,82±12,69; 44,10±13,50; 51,88± 20,14; 47,68±13,18 g/L pojedinačno. Kod teladi koja su napajana punim kolostrumom bez i sa dodatkom mineralnog adsorbenta (I i II grupa) utvrđena je statistička značajnost razlike u koncentraciji IgG u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu teladi koja su napajana kolostrumom sa dodatkom zeolita (II grupa) bila je oko 30% veća u odnosu na I grupu. Statistički značajna razlika u koncentraciji IgG utvrđena je i poređenjem I grupe sa III i IV u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu kod III grupe teladi bila je za 40% veća nego u I. Rezultati su ukazali da kod teladi koja su napajana kolostrumom visokog kvaliteta, u koji je dodat mineralni adsorbent, dolazi do maksimalne adsorpcije IgG. Određivanje proteinemije kod teladi u neonatalnom periodu je pouzdan pokazatelj uticaja mineralnog adsorbenta na resorpciju kolostralnih IgG. Koncentracija glukoze kod svih oglednih grupa se značajno povećava sa povećanjem broja uzetih kolostruma, bez većeg uticaja mineralnog adsorbenta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose
T1  - Efekat mineralnog adsorbenta na nivo serumskih imunoglobulina G, proteina i glukoze kod teladi na kolostralnoj ishrani
VL  - 57
IS  - 2-3
SP  - 169
EP  - 180
DO  - 10.2298/AVB0703169F
ER  - 

@article{
author = "Fratrić, Natalija and Stojić, Velibor and Rajčić, S. and Radojičić, Biljana",
year = "2007",
abstract = "The aim of the study was to investigate the influence of added natural mineral adsorbent zeolite (clinoptylolite) on the degree of absorption of immunoglobulin G and on glucose and protein concentration in the blood of calves during the neonatal period. Sixty calves were randomly divided in to four groups (15 calves per group). The quality and timing of colostrum feeding was as follows: group I was fed only with 1.5 L of colostrum at 2nd, 12th, 24th and 36th hour after birth; group II was fed with 1.5 L of colostrum with the mineral adsorbent added at a 5% concentration in the same time intervals; group III was fed with 1.5 L of colostrum together with the mineral adsorbent added at a 5% concentration, but at 2nd and 12 hour after birth it was fed with the first colostrum, and 24 and 36 hours after birth it was fed with the second colostrum. Group IV of calves was fed the same way as the third one only without the addition of the mineral adsorbent. Mean serum concentration of IgG was 15.14±7.43; 22.22±8.15; 23.65±9.93; 20.81±4.19 g/L in groups I to IV respectively at 6h of age. IgG concentration in the sera of all groups were 29.10±15.25; 45.46±13.45; 50.72±21.57; 41.60±15.42 g/L respectively at 16h after birth. At 30 hours of age, concentration of IgG was 33.29±14.70; 47.05±12.38; 55.00±19.71; 51.59±13.82 g/L in groups I to IV respectively. IgG concentration in the sera of all groups were 29.82±12.69; 44.10±13.50; 51.88±20.14; 47.68±13.18 g/L respectively at 40 hours after birth. For the calves which were fed with the full colostrum without and with the addition of the mineral adsorbent (Group I and II) the statistical significance of the difference in the concentration of IgG was determined during all the tested intervals. Concentration of IgG in the sera of group II calves (fed colostrum with zeolite added) was approximately 30% higher than in the group I. The statistical significance of the difference in levels of IgG between group I and groups III an IV was determined during all tested intervals, also. Concentration of IgG in the sera of group III calves was 40% higher than in group I. Acta Veterinaria (Beograd), Vol. 57, No. 2-3, 169-180, 2007. Results suggest that calves should receive feedings of high quality colostrum with added mineral adsorbent in order to maximize colostral IgG absorption. Determining the proteinemia in the neonatal period of calves is a reliable indicator of the impact of the mineral adsorbent on the degree of absorption of colostral IgG. The concentration of glucose for all the tested groups increased significantly with the increase of the quantity of ingested colostrum, with no evident influence of the added mineral adsorbent., Cilj ovog rada bio je da se ispita uticaj mineralnog adsorbenta na bazi klinoptilolita na stepen resorpcije IgG i koncentraciju nekih biohemijskih parametara u krvnom serumu teladi u neonatalnom periodu. Telad su bila podeljena u četiri grupe (po 15 teladi u svakoj grupi). I grupa teladi napajana je sa kolostrumom 2h, 12h, 24h i 36h posle rođenja, II grupa teladi napajana je sa kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% u istim vremenskim intervalima, III grupa napajana je sa 1,5 L kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% ali 2h i 12h posle rođenja napajana su prvim kolostrumom, a 24h i 36h dugim kolostrumom, IV grupa teladi hranjena je na isti način kao i III grupa samo što u kolostrum nije bio dodat mineralni adsorbent. Koncentracija IgG u krvnom serumu teladi od I do IV grupe, 6 sata iznosila je pojedinačno 15,14±7,43; 22,22±8,15; 23,65±9,93; 20,81±4,19 g/L. U krvnom serumu teladi kod svih ispitivanih grupa koncentracija IgG u 16h iznosila je pojedinač no 29,10±15,25; 45,46±13,45; 50,72±21,57; 41,60±15,42 g/L. Koncentracija IgG 30sata kod ispitivanih grupa teladi (I do IV) bila je pojedinačno 33,29±14,70; 47,05±12,38; 55,00±19,71; 51,59±13,82 g/L. Koncentracija IgG u krvnom serumu svih grupa 40h iznosila je 29,82±12,69; 44,10±13,50; 51,88± 20,14; 47,68±13,18 g/L pojedinačno. Kod teladi koja su napajana punim kolostrumom bez i sa dodatkom mineralnog adsorbenta (I i II grupa) utvrđena je statistička značajnost razlike u koncentraciji IgG u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu teladi koja su napajana kolostrumom sa dodatkom zeolita (II grupa) bila je oko 30% veća u odnosu na I grupu. Statistički značajna razlika u koncentraciji IgG utvrđena je i poređenjem I grupe sa III i IV u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu kod III grupe teladi bila je za 40% veća nego u I. Rezultati su ukazali da kod teladi koja su napajana kolostrumom visokog kvaliteta, u koji je dodat mineralni adsorbent, dolazi do maksimalne adsorpcije IgG. Određivanje proteinemije kod teladi u neonatalnom periodu je pouzdan pokazatelj uticaja mineralnog adsorbenta na resorpciju kolostralnih IgG. Koncentracija glukoze kod svih oglednih grupa se značajno povećava sa povećanjem broja uzetih kolostruma, bez većeg uticaja mineralnog adsorbenta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose, Efekat mineralnog adsorbenta na nivo serumskih imunoglobulina G, proteina i glukoze kod teladi na kolostralnoj ishrani",
volume = "57",
number = "2-3",
pages = "169-180",
doi = "10.2298/AVB0703169F"
}

Fratrić, N., Stojić, V., Rajčić, S.,& Radojičić, B.. (2007). The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(2-3), 169-180.
https://doi.org/10.2298/AVB0703169F

Fratrić N, Stojić V, Rajčić S, Radojičić B. The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose. in Acta Veterinaria-Beograd. 2007;57(2-3):169-180.
doi:10.2298/AVB0703169F .

Fratrić, Natalija, Stojić, Velibor, Rajčić, S., Radojičić, Biljana, "The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose" in Acta Veterinaria-Beograd, 57, no. 2-3 (2007):169-180,
https://doi.org/10.2298/AVB0703169F . .

TY  - JOUR
AU  - Kozmus, P.
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Stojić, Velibor
AU  - Kulišić, Zoran
AU  - Meglič, V.
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/468
AB  - In this study mitochondrial DNA of the indigenous honey bee populations from Serbia was analysed. Three ecotypes were comprised in the investigation: Banat (B), Syenichko-Peshterski (S) and Timok (T). The results show that all investigated samples of honey bees from Serbia belong to one mtDNA haplotype of the C phylogenetic lineage, previously designated as C2D haplotype, though with three additional polymorphic sites. For the novel mtDNA haplotype, the designation C2E is proposed. The novel C2E haplotype differs from C2D in two A-T transversions and in one insertion. Moreover, within the T honey bee ecotype, two additional polymorphic sites were observed; a deletion of a T nucleotide and a T-C transition. Our results suggest that the Carniolan honey bee populations from Serbia may represent an indigenous gene pool within A. m. carnica. The mtDNA haplotype (C2D), previously found in A. m. macedonica, was found in all samples of Serbian A. m. carnica. This suggests that the C2D haplotype may be found within both subspecies. This study represents the first effort to characterize the indigenous honey bee populations in Serbia using molecular techniques with the objective of improving our understanding of the phylogeography of Apis mellifera.
AB  - U ovom radu su prikazani rezultati analize mitohondrijalne DNK autohtonih populacija medonosne pčele iz Srbije. Istraživanje je obuhvatilo tri ekotipa: Banatski (B), Sjeničko-Pešterski (S) i Timočki (T). Rezultati ukazuju da svi ispitivani uzorci medonosne pčele iz Srbije pripadaju jednom mtDNK haplotipu C filogenetske linije, ranije označenom kao C2D haplotip, mada sa tri polimorfna mesta više. Za novi mtDNK haplotip predložena je oznaka C2E. Novi C2E haplotip razlikuje se od C2D haplotipa u dve A-T transverzije i jednoj inserciji. Osim toga, u okviru T ekotipa medonosne pčele uočena su još dva polimorfna mesta, delecija nukleotida T i T-C tranzicija. Ovi rezultati ukazuju da populacije kranjske medonosne pčele iz Srbije mogu pretstavljati autohtoni genski pul u okviru podvrste A. m. carnica. Mitohondrijalni DNK haplotip C2D, koji je prethodno opisan kod A. m. macedonica, utvrđen je u svim uzorcima A. m. carnica iz Srbije. Ovakav nalaz sugerise da C2D haplotip može postojati kod obe podvrste. Ovaj rad predstavlja prvi pokušaj da se obavi karakterizacija autohtonih populacija medonosne pčele u Srbiji primenom molekularnih tehnika, a u cilju boljeg razumevanja filogeografije vrste Apis mellifera.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia
T1  - Analiza mitohondrijalne DNK kod medonosnih pčela (Apis mellifera) iz Srbije
VL  - 57
IS  - 5-6
SP  - 465
EP  - 476
DO  - 10.2298/AVB0706465K
ER  - 

@article{
author = "Kozmus, P. and Stevanović, Jevrosima and Stanimirović, Zoran and Stojić, Velibor and Kulišić, Zoran and Meglič, V.",
year = "2007",
abstract = "In this study mitochondrial DNA of the indigenous honey bee populations from Serbia was analysed. Three ecotypes were comprised in the investigation: Banat (B), Syenichko-Peshterski (S) and Timok (T). The results show that all investigated samples of honey bees from Serbia belong to one mtDNA haplotype of the C phylogenetic lineage, previously designated as C2D haplotype, though with three additional polymorphic sites. For the novel mtDNA haplotype, the designation C2E is proposed. The novel C2E haplotype differs from C2D in two A-T transversions and in one insertion. Moreover, within the T honey bee ecotype, two additional polymorphic sites were observed; a deletion of a T nucleotide and a T-C transition. Our results suggest that the Carniolan honey bee populations from Serbia may represent an indigenous gene pool within A. m. carnica. The mtDNA haplotype (C2D), previously found in A. m. macedonica, was found in all samples of Serbian A. m. carnica. This suggests that the C2D haplotype may be found within both subspecies. This study represents the first effort to characterize the indigenous honey bee populations in Serbia using molecular techniques with the objective of improving our understanding of the phylogeography of Apis mellifera., U ovom radu su prikazani rezultati analize mitohondrijalne DNK autohtonih populacija medonosne pčele iz Srbije. Istraživanje je obuhvatilo tri ekotipa: Banatski (B), Sjeničko-Pešterski (S) i Timočki (T). Rezultati ukazuju da svi ispitivani uzorci medonosne pčele iz Srbije pripadaju jednom mtDNK haplotipu C filogenetske linije, ranije označenom kao C2D haplotip, mada sa tri polimorfna mesta više. Za novi mtDNK haplotip predložena je oznaka C2E. Novi C2E haplotip razlikuje se od C2D haplotipa u dve A-T transverzije i jednoj inserciji. Osim toga, u okviru T ekotipa medonosne pčele uočena su još dva polimorfna mesta, delecija nukleotida T i T-C tranzicija. Ovi rezultati ukazuju da populacije kranjske medonosne pčele iz Srbije mogu pretstavljati autohtoni genski pul u okviru podvrste A. m. carnica. Mitohondrijalni DNK haplotip C2D, koji je prethodno opisan kod A. m. macedonica, utvrđen je u svim uzorcima A. m. carnica iz Srbije. Ovakav nalaz sugerise da C2D haplotip može postojati kod obe podvrste. Ovaj rad predstavlja prvi pokušaj da se obavi karakterizacija autohtonih populacija medonosne pčele u Srbiji primenom molekularnih tehnika, a u cilju boljeg razumevanja filogeografije vrste Apis mellifera.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia, Analiza mitohondrijalne DNK kod medonosnih pčela (Apis mellifera) iz Srbije",
volume = "57",
number = "5-6",
pages = "465-476",
doi = "10.2298/AVB0706465K"
}

Kozmus, P., Stevanović, J., Stanimirović, Z., Stojić, V., Kulišić, Z.,& Meglič, V.. (2007). Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 465-476.
https://doi.org/10.2298/AVB0706465K

Kozmus P, Stevanović J, Stanimirović Z, Stojić V, Kulišić Z, Meglič V. Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia. in Acta Veterinaria-Beograd. 2007;57(5-6):465-476.
doi:10.2298/AVB0706465K .

Kozmus, P., Stevanović, Jevrosima, Stanimirović, Zoran, Stojić, Velibor, Kulišić, Zoran, Meglič, V., "Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):465-476,
https://doi.org/10.2298/AVB0706465K . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Pejin, Ivana I.
AU  - Kulišić, Zoran
AU  - Điporović, Milanka
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/480
AB  - Fumagillin is a naturally secreted antibiotic of the fungus Aspergillus fumigatus. In veterinary medicine fumagillin is used against nosemosis in bees and microsporidiosis in fish. Since fumagillin is stable in honey in a honey bee hive there is a possible genotoxic risk to beekeepers and consumers of honey contaminated with this substance. The genotoxic effect of fumagillin was evaluated in sisterchromatid exchange (SCE) and chromosome aberration tests in cultured human peripheral blood lymphocytes at three concentrations (1.02, 3.07 and 9.20 _g/mL), with 1:1 water-sugar syrup as the negative control and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) as the positive control. The mitotic and proliferative indices were calculated to detect the cytostatic effect of fumagillin. The results revealed that all tested concentrations of fumagillin (1.02, 3.07 and 9.20 _g/mL) significantly (p lt 0.001) increased the SCE frequency per cell and decreased the proliferative activity of human cultured lymphocytes which was manifested in the decrease in mitotic and proliferative indices. Significant increase (p lt 0.001) in aneuploidy and polyploidy was induced by medium and maximum concentrations of fumagillin. Besides, maximum concentrations of fumagillin significantly (p lt 0.001) increased the frequency of monitored structural aberrations of gap, break and insertion type. The results of this study demonstrate the genotoxic potential of fumagillin in vitro.
AB  - Fumagilin je prirodni antibiotik Aspergillus fumigatus-a. U veterinarskoj medicini fumagilin se koristi protiv nozemoza kod pčela i mikrosporidioza kod riba. Kako je fumagilin stabilan u medu i pčelinjim košnicama, postoji genotoksični rizik za pčelare i konzumere meda kontaminiranog ovom supstancom. Genotoksič ni efekat fumagilina je procenjivan testovima razmene sestrinskih hromatida i hromozomskih aberacija u kulturi limfocita periferne krvi ljudi u tri koncentracije (1,02, 3,07 and 9,20 _g/mL), gde je kao negativna kontrola korišćen vodeni rastvor šećernog sirupa, a kao pozitivna kontrola N-metil-N'-nitro-N-nitrozoguanidin (MNNG). Citostatični efekat fumagilina je utvrđivan mitotskim i proliferativnim indeksima. Rezultati ukazuju da sve testirane koncentracije fumagilina (1,02, 3,07 and 9,20 _g/mL) signifikantno (p lt 0,001) povećavaju frekvenciju SCE po ćeliji i smanjuju proliferativnu aktivnost kultivisanih ćelija koja je manifestovana smanjenjem mitotskih i proliferativnih indeksa. Signifikantno povećanje (p lt 0,001) aneuploidija i poliploidija je indukovano srednjom i maksimalnom koncentracijom fumagilina. Osim toga, maksimalna koncentracija fumagilina signifikantno (p lt 0,001) povećava frekvenciju praćenih strukturnih aberacija tipa gapova, prekida i insercija. Rezultati ove studije pokazuju genotoksični potencijal fumagilina in vitro.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures
T1  - Evaluacija genotoksičnog efekta fumagilina u kulturi humanih ćelija praćenjem razmene sestrinskih hromatida i hromozomskih aberacija
VL  - 57
IS  - 2-3
SP  - 257
EP  - 273
DO  - 10.2298/AVB0703257S
ER  - 

@article{
author = "Stanimirović, Zoran and Pejin, Ivana I. and Kulišić, Zoran and Điporović, Milanka",
year = "2007",
abstract = "Fumagillin is a naturally secreted antibiotic of the fungus Aspergillus fumigatus. In veterinary medicine fumagillin is used against nosemosis in bees and microsporidiosis in fish. Since fumagillin is stable in honey in a honey bee hive there is a possible genotoxic risk to beekeepers and consumers of honey contaminated with this substance. The genotoxic effect of fumagillin was evaluated in sisterchromatid exchange (SCE) and chromosome aberration tests in cultured human peripheral blood lymphocytes at three concentrations (1.02, 3.07 and 9.20 _g/mL), with 1:1 water-sugar syrup as the negative control and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) as the positive control. The mitotic and proliferative indices were calculated to detect the cytostatic effect of fumagillin. The results revealed that all tested concentrations of fumagillin (1.02, 3.07 and 9.20 _g/mL) significantly (p lt 0.001) increased the SCE frequency per cell and decreased the proliferative activity of human cultured lymphocytes which was manifested in the decrease in mitotic and proliferative indices. Significant increase (p lt 0.001) in aneuploidy and polyploidy was induced by medium and maximum concentrations of fumagillin. Besides, maximum concentrations of fumagillin significantly (p lt 0.001) increased the frequency of monitored structural aberrations of gap, break and insertion type. The results of this study demonstrate the genotoxic potential of fumagillin in vitro., Fumagilin je prirodni antibiotik Aspergillus fumigatus-a. U veterinarskoj medicini fumagilin se koristi protiv nozemoza kod pčela i mikrosporidioza kod riba. Kako je fumagilin stabilan u medu i pčelinjim košnicama, postoji genotoksični rizik za pčelare i konzumere meda kontaminiranog ovom supstancom. Genotoksič ni efekat fumagilina je procenjivan testovima razmene sestrinskih hromatida i hromozomskih aberacija u kulturi limfocita periferne krvi ljudi u tri koncentracije (1,02, 3,07 and 9,20 _g/mL), gde je kao negativna kontrola korišćen vodeni rastvor šećernog sirupa, a kao pozitivna kontrola N-metil-N'-nitro-N-nitrozoguanidin (MNNG). Citostatični efekat fumagilina je utvrđivan mitotskim i proliferativnim indeksima. Rezultati ukazuju da sve testirane koncentracije fumagilina (1,02, 3,07 and 9,20 _g/mL) signifikantno (p lt 0,001) povećavaju frekvenciju SCE po ćeliji i smanjuju proliferativnu aktivnost kultivisanih ćelija koja je manifestovana smanjenjem mitotskih i proliferativnih indeksa. Signifikantno povećanje (p lt 0,001) aneuploidija i poliploidija je indukovano srednjom i maksimalnom koncentracijom fumagilina. Osim toga, maksimalna koncentracija fumagilina signifikantno (p lt 0,001) povećava frekvenciju praćenih strukturnih aberacija tipa gapova, prekida i insercija. Rezultati ove studije pokazuju genotoksični potencijal fumagilina in vitro.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures, Evaluacija genotoksičnog efekta fumagilina u kulturi humanih ćelija praćenjem razmene sestrinskih hromatida i hromozomskih aberacija",
volume = "57",
number = "2-3",
pages = "257-273",
doi = "10.2298/AVB0703257S"
}

Stanimirović, Z., Pejin, I. I., Kulišić, Z.,& Điporović, M.. (2007). Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(2-3), 257-273.
https://doi.org/10.2298/AVB0703257S

Stanimirović Z, Pejin II, Kulišić Z, Điporović M. Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures. in Acta Veterinaria-Beograd. 2007;57(2-3):257-273.
doi:10.2298/AVB0703257S .

Stanimirović, Zoran, Pejin, Ivana I., Kulišić, Zoran, Điporović, Milanka, "Evaluation of genotoxic effects of fumagillin by sister chromatide exchange and chromosomal aberration tests in human cell cultures" in Acta Veterinaria-Beograd, 57, no. 2-3 (2007):257-273,
https://doi.org/10.2298/AVB0703257S . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Bajić, Vladan
AU  - Radović, Ivica
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/443
AB  - Fumagillin is a naturally secreted antibiotic of the fungus Aspergillus fumigates. It is used in veterinary medicine against microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin (in the form of fumagillin dicyclohexylamine) was evaluated in mouse bone-marrow cells using the mitotic index (MI), the chromosome aberration (CA) assay, and the micronucleus (MN) test. Fumagillin was administered to BALB/c mice by gavage, at doses of 25, 50, 75 mg/kg body weight (bw), repeated for 7 days at 24-h intervals, with water-sugar syrup as a negative control and cyclophosphamide (40 mg/kg bw) as a positive control. All experimental doses of fumagillin induced a significant decrease (p < 0.001) in MI (3.47 +/- 0.04%, 3.17 +/- 0.01%, and 2.27 +/- 0.02%, respectively) in comparison with the negative control (6.00 +/- 0.01%). Fumagillin significantly (p < 0.001) increased the frequency of MN (4.98 +/- 0.35, 8.45 +/- 0.57, and 12.02 +/- 0.37, respectively) over negative control (1.04 +/- 0.28). Significantly increased frequencies (p < 0.01 or p < 0.001) of numerical chromosomal aberrations (aneuploidies and polyploidies) and structural chromosomal aberrations such as gaps, breaks, and centric rings were observed at the highest experimental dose of fumagillin (75 mg/kg bw) compared with the negative control. However, with respect to the induction of Robertsonian translocations, both the intermediate (50 mg/kg bw) and highest (75 mg/kg bw) experimental dose caused a significant (p < 0.001) increase (7.12 +/- 0.26 and 9.00 +/- 0.10, respectively) in comparison with the negative control (0.00 +/- 0.00). Chromosomes 4 and 19 participated in these Robertsonian translocations. Regarding total cytogenetic changes, a significant increase (p < 0.001) was observed in both the intermediate dose group (17.36 +/- 1.83) and the highest dose group (59.49 +/- 1.92) compared with the negative control (7.00 +/- 1.35). These results suggest that fumagillin has genotoxic (clastogenic) potential in mammals in vivo.
PB  - Elsevier Science Bv, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo
VL  - 628
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1016/j.mrgentox.2006.09.014
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Bajić, Vladan and Radović, Ivica",
year = "2007",
abstract = "Fumagillin is a naturally secreted antibiotic of the fungus Aspergillus fumigates. It is used in veterinary medicine against microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin (in the form of fumagillin dicyclohexylamine) was evaluated in mouse bone-marrow cells using the mitotic index (MI), the chromosome aberration (CA) assay, and the micronucleus (MN) test. Fumagillin was administered to BALB/c mice by gavage, at doses of 25, 50, 75 mg/kg body weight (bw), repeated for 7 days at 24-h intervals, with water-sugar syrup as a negative control and cyclophosphamide (40 mg/kg bw) as a positive control. All experimental doses of fumagillin induced a significant decrease (p < 0.001) in MI (3.47 +/- 0.04%, 3.17 +/- 0.01%, and 2.27 +/- 0.02%, respectively) in comparison with the negative control (6.00 +/- 0.01%). Fumagillin significantly (p < 0.001) increased the frequency of MN (4.98 +/- 0.35, 8.45 +/- 0.57, and 12.02 +/- 0.37, respectively) over negative control (1.04 +/- 0.28). Significantly increased frequencies (p < 0.01 or p < 0.001) of numerical chromosomal aberrations (aneuploidies and polyploidies) and structural chromosomal aberrations such as gaps, breaks, and centric rings were observed at the highest experimental dose of fumagillin (75 mg/kg bw) compared with the negative control. However, with respect to the induction of Robertsonian translocations, both the intermediate (50 mg/kg bw) and highest (75 mg/kg bw) experimental dose caused a significant (p < 0.001) increase (7.12 +/- 0.26 and 9.00 +/- 0.10, respectively) in comparison with the negative control (0.00 +/- 0.00). Chromosomes 4 and 19 participated in these Robertsonian translocations. Regarding total cytogenetic changes, a significant increase (p < 0.001) was observed in both the intermediate dose group (17.36 +/- 1.83) and the highest dose group (59.49 +/- 1.92) compared with the negative control (7.00 +/- 1.35). These results suggest that fumagillin has genotoxic (clastogenic) potential in mammals in vivo.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo",
volume = "628",
number = "1",
pages = "1-10",
doi = "10.1016/j.mrgentox.2006.09.014"
}

Stanimirović, Z., Stevanović, J., Bajić, V.,& Radović, I.. (2007). Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier Science Bv, Amsterdam., 628(1), 1-10.
https://doi.org/10.1016/j.mrgentox.2006.09.014

Stanimirović Z, Stevanović J, Bajić V, Radović I. Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2007;628(1):1-10.
doi:10.1016/j.mrgentox.2006.09.014 .

Stanimirović, Zoran, Stevanović, Jevrosima, Bajić, Vladan, Radović, Ivica, "Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 628, no. 1 (2007):1-10,
https://doi.org/10.1016/j.mrgentox.2006.09.014 . .

TY  - JOUR
AU  - Jezdimirović, Milanka
AU  - Kulišić, Zoran
AU  - Aleksić, Nevenka
AU  - Bjelić, Nebojša
AU  - Ivanović, Saša
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/416
AB  - The acaricide efficacy, tolerability and safety of the active ingredient of the etheric oil of cloves eugenol was investigated in the treatment of mange in swine, and the obtained results were compared with the results of acaricide efficacy of the synthetic acaricide permethrin, which has been in use for quite a some time. A single application of permethrin in the form of a 1% solution showed maximum efficacy of 62.5%, and after three applications of 75.0% in the treatment of sarcoptes in swine mange. A single application of eugenol in the form of a 10% solution had maximum efficacy of 75.0%, and applied three times an efficacy of 100% in curbing Sarcoptes scabiei var. suis. A single administration of 20% eugenol solution showed maximum efficacy of 87.5%, and applied three times it was 100% efficient in curbing Sarcoptes scabeiei var. suis. The best efficacy in the treatment of sarcoptes mange in swine was achieved with three applications of eugenol in a concentration of 20%. This maximum effect (100%) was obtained already after the second treatment. Eugenol in a concentration of 10% was safe for local application on skin because it does not cause any undesired reactions, while a 20% concentration caused irritation followed by a passing redness and disquiet in a smaller number of treated animals. The results of comparative investigations of acaricide efficacy of permethrin and eugenol demonstrate that there is resistence in Sarcoptes scabiei var. suis to permethrin. The biocide eugenol can safely be recommended for the treatment of sarcoptes mange in swine.
AB  - Ispitivana je akaricidna efikasnost, podnošljivost i bezbednost aktivnog sastojka eteričnog ulja karanfilića, eugenola, u lečenju šuge svinja i dobijeni rezultati su upoređeni sa rezultatima akaricidne efikasnosti, već duže vreme korišćenog sintetskog akaricida permetrina. Jednokratno primenjen permetrin u obliku rastvora koncentracije od 1% pokazao je maksimalnu efikasnost od 62,5%, a posle trokratne aplikacije od 75,0% u lečenju sarkoptes šuge svinja. Jednokratno primenjen eugenol u obliku rastvora koncentracije od 10% imao je maksimalnu efikasnost od 75,0%, a trokratno primenjen od 100,0% u suzbijanju Sarcoptes scabiei var. suis. Jednokratno primenjen rastvor eugenola u koncentraciji od 20% pokazao je najveću efikasnost od 87,5%, a trokratno primenjen od 100,0% u suzbijanju sa Sarcoptes scabiei var. suis. Najbolja efikasnost u lečenju sarkoptes šuge svinja je postignuta trokratnom primenom eugenola u koncentraciji od 20%. Ovaj maksimalni efekat (100,0%) dobijen je već posle drugog tretmana. Eugenol u koncentraciji od 10% bio je bezbedan za lokalnu primenu na koži, jer ne prouzrokuje bilo kakve neželjene reakcije, dok u koncentraciji od 20% kod manjeg broja tretiranih životinja izazivao je iritaciju praćenu prolaznim crvenilom i uznemirenošću. Rezultati uporednih ispitivanja akaricidne efikasnosti permetrina i eugenola ukazuju da postoji rezistencija Sarcoptes scabiei var. suis na permetrin. Biocid eugenol sa sigurnošću može da se preporuči u lečenju sarkoptes šuge svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of eugenol efficacy in treatment of mange in swine
T1  - Efikasnost eugenola u lečenju šuge svinja
VL  - 60
IS  - 1-2
SP  - 33
EP  - 42
DO  - 10.2298/VETGL0602033J
ER  - 

@article{
author = "Jezdimirović, Milanka and Kulišić, Zoran and Aleksić, Nevenka and Bjelić, Nebojša and Ivanović, Saša",
year = "2006",
abstract = "The acaricide efficacy, tolerability and safety of the active ingredient of the etheric oil of cloves eugenol was investigated in the treatment of mange in swine, and the obtained results were compared with the results of acaricide efficacy of the synthetic acaricide permethrin, which has been in use for quite a some time. A single application of permethrin in the form of a 1% solution showed maximum efficacy of 62.5%, and after three applications of 75.0% in the treatment of sarcoptes in swine mange. A single application of eugenol in the form of a 10% solution had maximum efficacy of 75.0%, and applied three times an efficacy of 100% in curbing Sarcoptes scabiei var. suis. A single administration of 20% eugenol solution showed maximum efficacy of 87.5%, and applied three times it was 100% efficient in curbing Sarcoptes scabeiei var. suis. The best efficacy in the treatment of sarcoptes mange in swine was achieved with three applications of eugenol in a concentration of 20%. This maximum effect (100%) was obtained already after the second treatment. Eugenol in a concentration of 10% was safe for local application on skin because it does not cause any undesired reactions, while a 20% concentration caused irritation followed by a passing redness and disquiet in a smaller number of treated animals. The results of comparative investigations of acaricide efficacy of permethrin and eugenol demonstrate that there is resistence in Sarcoptes scabiei var. suis to permethrin. The biocide eugenol can safely be recommended for the treatment of sarcoptes mange in swine., Ispitivana je akaricidna efikasnost, podnošljivost i bezbednost aktivnog sastojka eteričnog ulja karanfilića, eugenola, u lečenju šuge svinja i dobijeni rezultati su upoređeni sa rezultatima akaricidne efikasnosti, već duže vreme korišćenog sintetskog akaricida permetrina. Jednokratno primenjen permetrin u obliku rastvora koncentracije od 1% pokazao je maksimalnu efikasnost od 62,5%, a posle trokratne aplikacije od 75,0% u lečenju sarkoptes šuge svinja. Jednokratno primenjen eugenol u obliku rastvora koncentracije od 10% imao je maksimalnu efikasnost od 75,0%, a trokratno primenjen od 100,0% u suzbijanju Sarcoptes scabiei var. suis. Jednokratno primenjen rastvor eugenola u koncentraciji od 20% pokazao je najveću efikasnost od 87,5%, a trokratno primenjen od 100,0% u suzbijanju sa Sarcoptes scabiei var. suis. Najbolja efikasnost u lečenju sarkoptes šuge svinja je postignuta trokratnom primenom eugenola u koncentraciji od 20%. Ovaj maksimalni efekat (100,0%) dobijen je već posle drugog tretmana. Eugenol u koncentraciji od 10% bio je bezbedan za lokalnu primenu na koži, jer ne prouzrokuje bilo kakve neželjene reakcije, dok u koncentraciji od 20% kod manjeg broja tretiranih životinja izazivao je iritaciju praćenu prolaznim crvenilom i uznemirenošću. Rezultati uporednih ispitivanja akaricidne efikasnosti permetrina i eugenola ukazuju da postoji rezistencija Sarcoptes scabiei var. suis na permetrin. Biocid eugenol sa sigurnošću može da se preporuči u lečenju sarkoptes šuge svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of eugenol efficacy in treatment of mange in swine, Efikasnost eugenola u lečenju šuge svinja",
volume = "60",
number = "1-2",
pages = "33-42",
doi = "10.2298/VETGL0602033J"
}

Jezdimirović, M., Kulišić, Z., Aleksić, N., Bjelić, N.,& Ivanović, S.. (2006). Investigations of eugenol efficacy in treatment of mange in swine. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 33-42.
https://doi.org/10.2298/VETGL0602033J

Jezdimirović M, Kulišić Z, Aleksić N, Bjelić N, Ivanović S. Investigations of eugenol efficacy in treatment of mange in swine. in Veterinarski Glasnik. 2006;60(1-2):33-42.
doi:10.2298/VETGL0602033J .

Jezdimirović, Milanka, Kulišić, Zoran, Aleksić, Nevenka, Bjelić, Nebojša, Ivanović, Saša, "Investigations of eugenol efficacy in treatment of mange in swine" in Veterinarski Glasnik, 60, no. 1-2 (2006):33-42,
https://doi.org/10.2298/VETGL0602033J . .

TY  - JOUR
AU  - Stojiljković, Dragoslav
AU  - Aleksić, Nevenka
AU  - Kulišić, Zoran
AU  - Nikolovski-Stefanović, Zorica
AU  - Mišić, Zorana
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/397
AB  - This paper describes cases of demodicosis in dogs from the territory of the city of Niš. The objective of the investigations was to learn more about the parameters that can influence the susceptibility of certain dogs to the cause of demodicosis, such as: age, hair length, sex, and seasonal dynamics of the incidence of the disease. It was established following examinations of 76 dogs suspected of demodicosis that they were all infected with the specie Demodex canis. The changes were localized or generalized. In the total number of infected dogs, there was a larger share of males (61.84%) than females (38.16%). The number of dogs diseased with demodicosis was twice higher in shorthaired than in long-haired animals. The most frequently infected were dogs of the Doberman and German Shepherd breeds. The number of male and female animals among short-haired dogs diseased with demodicosis was equal, while there were more males among the long-haired animals. The biggest number of infected animals was in the category of young dogs, up to one year of age, and they accounted for 65.79% of the total number of diseased animals, which shows without any doubt that younger dogs are more susceptible to demodicosis. There were more infected dogs in the period autumn-winter than in the period spring-summer.
AB  - U ovom radu opisani su slučajevi demodikoze kod pasa sa teritorije grada Niša. Cilj ispitivanja je bio da se nešto više sazna o parametrima koji mogu da utiču na prijemčivost određenih pasa prema uzročniku demodikoze kao što su: životno doba, dužina dlake, pol i sezonska dinamika pojavljivanja bolesti. Pregledom 76 pasa suspektnih na demodikozu, ustanovljeno je da su svi bili infestirani vrstom Demodex canis. Promene su bile lokalizovane ili generalizovane. U ukupnom broju infestiranih pasa veći je bio udeo mužjaka (61,84%) nego ženki (38,16%). Broj pasa obolelih od demodikoze bio je dva puta veći kod kratkodlakih u odnosu na dugodlake pse. Najčešće infestirani su bili psi rase doberman i nemački ovčar. Broj muških i ženskih jedinki kod kratkodlakih pasa obolelih od demodikoze bio je ujednačen, dok je kod dugodlakih bio veći broj mužjaka. Najveći broj infestiranih je bio iz kategorije mladih pasa, uzrasta od godinu dana i njihov udeo u ukupnom broju obolelih bio je 65,79 posto, što nesumnjivo ukazuje da su demodikozi podložniji mlađi psi. U periodu jesen-zima bio je veći broj infestiranih pasa nego u periodu proleće-leto.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Canine demodicosis in territory of city of Niš
T1  - Demodikoza pasa na teritoriji grada Niša
VL  - 60
IS  - 5-6
SP  - 397
EP  - 405
DO  - 10.2298/VETGL0606397S
ER  - 

@article{
author = "Stojiljković, Dragoslav and Aleksić, Nevenka and Kulišić, Zoran and Nikolovski-Stefanović, Zorica and Mišić, Zorana",
year = "2006",
abstract = "This paper describes cases of demodicosis in dogs from the territory of the city of Niš. The objective of the investigations was to learn more about the parameters that can influence the susceptibility of certain dogs to the cause of demodicosis, such as: age, hair length, sex, and seasonal dynamics of the incidence of the disease. It was established following examinations of 76 dogs suspected of demodicosis that they were all infected with the specie Demodex canis. The changes were localized or generalized. In the total number of infected dogs, there was a larger share of males (61.84%) than females (38.16%). The number of dogs diseased with demodicosis was twice higher in shorthaired than in long-haired animals. The most frequently infected were dogs of the Doberman and German Shepherd breeds. The number of male and female animals among short-haired dogs diseased with demodicosis was equal, while there were more males among the long-haired animals. The biggest number of infected animals was in the category of young dogs, up to one year of age, and they accounted for 65.79% of the total number of diseased animals, which shows without any doubt that younger dogs are more susceptible to demodicosis. There were more infected dogs in the period autumn-winter than in the period spring-summer., U ovom radu opisani su slučajevi demodikoze kod pasa sa teritorije grada Niša. Cilj ispitivanja je bio da se nešto više sazna o parametrima koji mogu da utiču na prijemčivost određenih pasa prema uzročniku demodikoze kao što su: životno doba, dužina dlake, pol i sezonska dinamika pojavljivanja bolesti. Pregledom 76 pasa suspektnih na demodikozu, ustanovljeno je da su svi bili infestirani vrstom Demodex canis. Promene su bile lokalizovane ili generalizovane. U ukupnom broju infestiranih pasa veći je bio udeo mužjaka (61,84%) nego ženki (38,16%). Broj pasa obolelih od demodikoze bio je dva puta veći kod kratkodlakih u odnosu na dugodlake pse. Najčešće infestirani su bili psi rase doberman i nemački ovčar. Broj muških i ženskih jedinki kod kratkodlakih pasa obolelih od demodikoze bio je ujednačen, dok je kod dugodlakih bio veći broj mužjaka. Najveći broj infestiranih je bio iz kategorije mladih pasa, uzrasta od godinu dana i njihov udeo u ukupnom broju obolelih bio je 65,79 posto, što nesumnjivo ukazuje da su demodikozi podložniji mlađi psi. U periodu jesen-zima bio je veći broj infestiranih pasa nego u periodu proleće-leto.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Canine demodicosis in territory of city of Niš, Demodikoza pasa na teritoriji grada Niša",
volume = "60",
number = "5-6",
pages = "397-405",
doi = "10.2298/VETGL0606397S"
}

Stojiljković, D., Aleksić, N., Kulišić, Z., Nikolovski-Stefanović, Z.,& Mišić, Z.. (2006). Canine demodicosis in territory of city of Niš. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 397-405.
https://doi.org/10.2298/VETGL0606397S

Stojiljković D, Aleksić N, Kulišić Z, Nikolovski-Stefanović Z, Mišić Z. Canine demodicosis in territory of city of Niš. in Veterinarski Glasnik. 2006;60(5-6):397-405.
doi:10.2298/VETGL0606397S .

Stojiljković, Dragoslav, Aleksić, Nevenka, Kulišić, Zoran, Nikolovski-Stefanović, Zorica, Mišić, Zorana, "Canine demodicosis in territory of city of Niš" in Veterinarski Glasnik, 60, no. 5-6 (2006):397-405,
https://doi.org/10.2298/VETGL0606397S . .

TY  - JOUR
AU  - Mišić, Zorana
AU  - Katić-Radivojević, Sofija
AU  - Kulišić, Zoran
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/405
AB  - The prevalence of Cryptosporidium infection among lambs and goat kids in Serbia was examined. The animals were grouped according to age, i.e., up to 30 days and from 31 to 90 days. The infection was diagnosed using three coprological procedures. Sheather’s sugar flotation was employed to determine the concentration of oocysts. Modified Ziehl-Neelsen technique and modified Kinyoun technique were used as staining procedures. Cryptosporidium oocysts were detected in 42.1% examined lambs and 31.8% goat kids. These findings clearly demonstrate the presence of Cryptospodium infection in lambs and goat kids in Serbia and indicate the potential role of these animals as reservoirs of cryptosporidia.
AB  - U ovom radu su izneti rezultati Ispitivanja raširenosti Cryptosporidium infekcije kod jagnjadi i jaradi na teritoriji Srbije. Ispitivane životinje su podeljene u dve starosne grupe: do 30 dana i od 31-90 dana. Za dijagnostiku kriptosporodijalnih infekcija korišćene su tri koprološke metode: flotacija po Sheatheru za koncentraciju oocista i modifikovane Ziehl-Neelsen i Kinyoun tehnike kao metode bojenja. Oociste kriptosporidija su ustanovljene kod 42,1% pregledane jagnjadi i 31,8% jaradi. Ovi nalazi ukazuju na potencijalnu ulogu jagnjadi i jaradi kao rezervoara infekcije za kriptosporidije drugih vrsta životinja i ljudi u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cryptosporidium infection in lambs and goat kids in Serbia
T1  - Cryptosporidium infekcija kod jagnjadi i jaradi u Srbiji
VL  - 56
IS  - 1
SP  - 49
EP  - 54
DO  - 10.2298/AVB0601049M
ER  - 

@article{
author = "Mišić, Zorana and Katić-Radivojević, Sofija and Kulišić, Zoran",
year = "2006",
abstract = "The prevalence of Cryptosporidium infection among lambs and goat kids in Serbia was examined. The animals were grouped according to age, i.e., up to 30 days and from 31 to 90 days. The infection was diagnosed using three coprological procedures. Sheather’s sugar flotation was employed to determine the concentration of oocysts. Modified Ziehl-Neelsen technique and modified Kinyoun technique were used as staining procedures. Cryptosporidium oocysts were detected in 42.1% examined lambs and 31.8% goat kids. These findings clearly demonstrate the presence of Cryptospodium infection in lambs and goat kids in Serbia and indicate the potential role of these animals as reservoirs of cryptosporidia., U ovom radu su izneti rezultati Ispitivanja raširenosti Cryptosporidium infekcije kod jagnjadi i jaradi na teritoriji Srbije. Ispitivane životinje su podeljene u dve starosne grupe: do 30 dana i od 31-90 dana. Za dijagnostiku kriptosporodijalnih infekcija korišćene su tri koprološke metode: flotacija po Sheatheru za koncentraciju oocista i modifikovane Ziehl-Neelsen i Kinyoun tehnike kao metode bojenja. Oociste kriptosporidija su ustanovljene kod 42,1% pregledane jagnjadi i 31,8% jaradi. Ovi nalazi ukazuju na potencijalnu ulogu jagnjadi i jaradi kao rezervoara infekcije za kriptosporidije drugih vrsta životinja i ljudi u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cryptosporidium infection in lambs and goat kids in Serbia, Cryptosporidium infekcija kod jagnjadi i jaradi u Srbiji",
volume = "56",
number = "1",
pages = "49-54",
doi = "10.2298/AVB0601049M"
}

Mišić, Z., Katić-Radivojević, S.,& Kulišić, Z.. (2006). Cryptosporidium infection in lambs and goat kids in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(1), 49-54.
https://doi.org/10.2298/AVB0601049M

Mišić Z, Katić-Radivojević S, Kulišić Z. Cryptosporidium infection in lambs and goat kids in Serbia. in Acta Veterinaria-Beograd. 2006;56(1):49-54.
doi:10.2298/AVB0601049M .

Mišić, Zorana, Katić-Radivojević, Sofija, Kulišić, Zoran, "Cryptosporidium infection in lambs and goat kids in Serbia" in Acta Veterinaria-Beograd, 56, no. 1 (2006):49-54,
https://doi.org/10.2298/AVB0601049M . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Pejin, Ivana I.
AU  - Lazarević, Miodrag
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/420
AB  - Fumagillin (dicyclohexylamine) is a natural antibiotic, secreted by Aspergillus fumigatus. It is used in veterinary medicine against microsporidiosis in bees and fish, as well as in human medicine for the treatment of intestinal amebiasis, microsporidial keratoconjunctivitis and intestinal microsporidiosis due to Enterocytozoon bieneusi in patients with AIDS and other types of immunodeficiency. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using the mitotic index (MI) and micronucleus (MN) assay. Fumagillin was administered to BALB/c mice by gavage in doses of 25, 50, 75 mg/kg b.w., repeated for 7 days at 24h intervals, with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) All experimental doses of fumagillin induced a significant decrease (p lt 0.001) in MI (3.47 ± 0.04%, 3.17 ± 0.01% and 2.27 ± 0.02%, respectively) in comparison with the negative control (6.00 ± 0.01%) and with the positive control (14.78 ± 0.09). Fumagillin significantly (p lt 0.001) increased the frequency of MN (4.98 ± 0.35, 8.45 ± 0.57 and 12.02 ± 0.37, respectively) over the negative control (1.04 ± 0.28). These results suggest that fumagillin (dicyclohexilamine) has an antiproliferative and genotoxic potential in mammal in vivo test.
AB  - Fumagilin (dicyclohexylamine) je prirodni antibiotik koga produkuje Aspergillus fumigatus. Upotrebljava se u veterinarskoj medicini protiv mikrosporidioza pčela i riba, ali i u humanoj medicini za tretman intestinalnih amebijaza, microsporidijalnih keratokonjunktivitisa i intestinalnih mikrosporidioza uzrokovanih sa Enterocytozoon bieneusi kod pacijenata obolelih od AIDS-a i drugih tipova imunodeficijencije. U ovom radu procenjivan je genotoksični potencijal fumagilina u ćelijama kostne srži miševa praćenjem mitotskog indeksa i učestalosti pojave mikronukleusa u polihromatofilnim eritrocitima. Fumagilinom su u 7-dnevnom tretmanu tretirani BALB/c miševi intragastrično u dozama 25, 50, 75 mg/kg TM, u intervalima od 24h. Kao negativna kontrola korišćen je vodeno-šećerni sirup dok je ciklofosfamid (40 mg/kg TM) korišćen kao pozitivna kontrola. Sve eksperimentalne doze fumagilina su izazvale signifikantno smanjenje (p lt 0.001) mitotskog indeksa (3.47 ± 0.04%, 3.17 ± 0.01% i 2.27 ± 0.02% respektivno) u poređenju sa negativnom kontrolom (6.00 ± 0.01%), kao i u odnosu na pozitivnu kontrolu (14.78 ± 0.09). Fumagilin je signifikantno (p lt 0.001) povećao frekvenciju pojave mikronukleusa (4.98± 0.35, 8.45 ± 0.57 i 12.02± 0.37, respektivno) u odnosu na negativnu kontrolu (1.04 ± 0.28). Ovi rezultati ukazuju da fumagilin (dicyclohexilamine) ispoljava antiproliferativni (citotoksični) i genotoksični potencijal u sisarskom in vivo testu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo
T1  - Praćenje mitotskog indeksa i učestalosti mikronukleusa u funkciji procene genotoksičnog potencijala fumagilina (dicikloheksilamina) in vivo
VL  - 56
IS  - 5-6
SP  - 437
EP  - 448
DO  - 10.2298/AVB0606437S
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Pejin, Ivana I. and Lazarević, Miodrag",
year = "2006",
abstract = "Fumagillin (dicyclohexylamine) is a natural antibiotic, secreted by Aspergillus fumigatus. It is used in veterinary medicine against microsporidiosis in bees and fish, as well as in human medicine for the treatment of intestinal amebiasis, microsporidial keratoconjunctivitis and intestinal microsporidiosis due to Enterocytozoon bieneusi in patients with AIDS and other types of immunodeficiency. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using the mitotic index (MI) and micronucleus (MN) assay. Fumagillin was administered to BALB/c mice by gavage in doses of 25, 50, 75 mg/kg b.w., repeated for 7 days at 24h intervals, with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) All experimental doses of fumagillin induced a significant decrease (p lt 0.001) in MI (3.47 ± 0.04%, 3.17 ± 0.01% and 2.27 ± 0.02%, respectively) in comparison with the negative control (6.00 ± 0.01%) and with the positive control (14.78 ± 0.09). Fumagillin significantly (p lt 0.001) increased the frequency of MN (4.98 ± 0.35, 8.45 ± 0.57 and 12.02 ± 0.37, respectively) over the negative control (1.04 ± 0.28). These results suggest that fumagillin (dicyclohexilamine) has an antiproliferative and genotoxic potential in mammal in vivo test., Fumagilin (dicyclohexylamine) je prirodni antibiotik koga produkuje Aspergillus fumigatus. Upotrebljava se u veterinarskoj medicini protiv mikrosporidioza pčela i riba, ali i u humanoj medicini za tretman intestinalnih amebijaza, microsporidijalnih keratokonjunktivitisa i intestinalnih mikrosporidioza uzrokovanih sa Enterocytozoon bieneusi kod pacijenata obolelih od AIDS-a i drugih tipova imunodeficijencije. U ovom radu procenjivan je genotoksični potencijal fumagilina u ćelijama kostne srži miševa praćenjem mitotskog indeksa i učestalosti pojave mikronukleusa u polihromatofilnim eritrocitima. Fumagilinom su u 7-dnevnom tretmanu tretirani BALB/c miševi intragastrično u dozama 25, 50, 75 mg/kg TM, u intervalima od 24h. Kao negativna kontrola korišćen je vodeno-šećerni sirup dok je ciklofosfamid (40 mg/kg TM) korišćen kao pozitivna kontrola. Sve eksperimentalne doze fumagilina su izazvale signifikantno smanjenje (p lt 0.001) mitotskog indeksa (3.47 ± 0.04%, 3.17 ± 0.01% i 2.27 ± 0.02% respektivno) u poređenju sa negativnom kontrolom (6.00 ± 0.01%), kao i u odnosu na pozitivnu kontrolu (14.78 ± 0.09). Fumagilin je signifikantno (p lt 0.001) povećao frekvenciju pojave mikronukleusa (4.98± 0.35, 8.45 ± 0.57 i 12.02± 0.37, respektivno) u odnosu na negativnu kontrolu (1.04 ± 0.28). Ovi rezultati ukazuju da fumagilin (dicyclohexilamine) ispoljava antiproliferativni (citotoksični) i genotoksični potencijal u sisarskom in vivo testu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo, Praćenje mitotskog indeksa i učestalosti mikronukleusa u funkciji procene genotoksičnog potencijala fumagilina (dicikloheksilamina) in vivo",
volume = "56",
number = "5-6",
pages = "437-448",
doi = "10.2298/AVB0606437S"
}

Stevanović, J., Stanimirović, Z., Pejin, I. I.,& Lazarević, M.. (2006). Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(5-6), 437-448.
https://doi.org/10.2298/AVB0606437S

Stevanović J, Stanimirović Z, Pejin II, Lazarević M. Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo. in Acta Veterinaria-Beograd. 2006;56(5-6):437-448.
doi:10.2298/AVB0606437S .

Stevanović, Jevrosima, Stanimirović, Zoran, Pejin, Ivana I., Lazarević, Miodrag, "Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo" in Acta Veterinaria-Beograd, 56, no. 5-6 (2006):437-448,
https://doi.org/10.2298/AVB0606437S . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Kulić, Milan
AU  - Stojić, Velibor
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/423
AB  - Dicyclohexylamine (fumagillin), as an antibiotic produced by fermentation of Aspergillus fumigatus, is used in human medicine for the treatment of intestinal microsporidiosis in patients with HIV infection, intestinal amebiasis and microsporidial keratoconjunctivitis. In veterinary medicine fumagillin is effective in suppressing microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using chromosome aberrations (CA) assay. Dicyclohexylamine was administered to mice by gavage in a dose of 25, 50, 75 mg/kg b.w., with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) Significantly increased frequency (p lt 0.01 or p lt 0.001) of numerical chromosomal aberrations (aneupliodies and polyploidies) and structural chromosomal aberrations of gaps, breaks and centric rings were observed only at the highest experimental dose of dicyclohexylamine, compared with the negative control. However, in point of induction of Rb translocations, both the median (50 mg/kg b.w) and highest (75 mg/kg b.w) experimental dose showed a significant (p lt 00.001) increase (7.12 ± 0.26 and 9.00 ± 0.10, respectively) in comparison with the negative control (0.00 ± 0.00). Chromosomes 4 and 19 participated in these Rb translocations. These results suggest that dicyclohexilamine (fumagillin) has genotoxic potential in mammal in vivo chromosomal aberration (CA) test system.
AB  - Dicyclohexylamin (fumagilin), kao antibiotik koga proizvodi gljivica Aspergillus fumigatus, se koristi u humanoj medicini u borbi protiv intestinalnih mikrosporidioza kod pacijenata sa HIV infekcijom, ali i za tretman obolelih od intestinalnih amebijaza i mikrosporidijanog keratokonjunktivitisa. U veterinarskoj medicini efikasan je u lečenju mikrosporidioza pčela i drugih insekata uzrokovanih sa Nosema apis, N. cerani, N. bombici, ali i mikrosporidioza riba uzrokovanih sa Myxobolus cerebralis i Tetracapsuloides bryosalmonae. U ovom radu ispitivana je genotoksičnost dicikloheksilamina kvantifikacijom i kvalifikacijom hromozomskih aberacija u ćelijama kostne srži miševa soja BALB/c. Dicikloheksilamin je aplikovan miševima intragastrično u dozama od 25, 50, 75 mg/kg b.w., pri čemu je vodeno-šećerni sirup predstavljao negativnu, a cyclophosphamid (40 mg/kg b.w) poozitivnu kontrolu. Utvrđeno je da samo najveća doza dicikloheksilamina signifikantno povećava učestalost (p lt 0.01 ili p lt 0.001) numeričkih hromozomskih aberacija (aneupliodija i poliploidija) i strukturnih hromozomskih aberacija tipa gapova, prekida i centričnih ringova u poređenju sa negativnom kontrolom. Međutim, srednja (50 mg/kg b.w) i najviša (75 mg/kg b.w) eksperimentalna doza izazvale su signifikantno (p lt 0.001) povećanje (7.12 ± 0.26 i 9.00 ± 0.10, respektivno) Robetrsonovih (Rb) translokacija u poređenju sa negativnom kontrolom (0.00 ± 0.00). U formiranju ovih Rb translokacija utvrđeno je da učestvuju hromozomi 4 i 19. Ovi rezultati sugerišu da je dicikloheksilamin (fumagilin) ispoljio visok genotoksični potencijal sa sposobnošću indukcije i Rb. Translokacija.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo
T1  - Učestalost hromozomskih aberacija u funkciji evaluacije genotoksičnog potencijala fumagilina in vivo
VL  - 56
IS  - 4
SP  - 353
EP  - 366
DO  - 10.2298/AVB0604353S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Kulić, Milan and Stojić, Velibor",
year = "2006",
abstract = "Dicyclohexylamine (fumagillin), as an antibiotic produced by fermentation of Aspergillus fumigatus, is used in human medicine for the treatment of intestinal microsporidiosis in patients with HIV infection, intestinal amebiasis and microsporidial keratoconjunctivitis. In veterinary medicine fumagillin is effective in suppressing microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using chromosome aberrations (CA) assay. Dicyclohexylamine was administered to mice by gavage in a dose of 25, 50, 75 mg/kg b.w., with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) Significantly increased frequency (p lt 0.01 or p lt 0.001) of numerical chromosomal aberrations (aneupliodies and polyploidies) and structural chromosomal aberrations of gaps, breaks and centric rings were observed only at the highest experimental dose of dicyclohexylamine, compared with the negative control. However, in point of induction of Rb translocations, both the median (50 mg/kg b.w) and highest (75 mg/kg b.w) experimental dose showed a significant (p lt 00.001) increase (7.12 ± 0.26 and 9.00 ± 0.10, respectively) in comparison with the negative control (0.00 ± 0.00). Chromosomes 4 and 19 participated in these Rb translocations. These results suggest that dicyclohexilamine (fumagillin) has genotoxic potential in mammal in vivo chromosomal aberration (CA) test system., Dicyclohexylamin (fumagilin), kao antibiotik koga proizvodi gljivica Aspergillus fumigatus, se koristi u humanoj medicini u borbi protiv intestinalnih mikrosporidioza kod pacijenata sa HIV infekcijom, ali i za tretman obolelih od intestinalnih amebijaza i mikrosporidijanog keratokonjunktivitisa. U veterinarskoj medicini efikasan je u lečenju mikrosporidioza pčela i drugih insekata uzrokovanih sa Nosema apis, N. cerani, N. bombici, ali i mikrosporidioza riba uzrokovanih sa Myxobolus cerebralis i Tetracapsuloides bryosalmonae. U ovom radu ispitivana je genotoksičnost dicikloheksilamina kvantifikacijom i kvalifikacijom hromozomskih aberacija u ćelijama kostne srži miševa soja BALB/c. Dicikloheksilamin je aplikovan miševima intragastrično u dozama od 25, 50, 75 mg/kg b.w., pri čemu je vodeno-šećerni sirup predstavljao negativnu, a cyclophosphamid (40 mg/kg b.w) poozitivnu kontrolu. Utvrđeno je da samo najveća doza dicikloheksilamina signifikantno povećava učestalost (p lt 0.01 ili p lt 0.001) numeričkih hromozomskih aberacija (aneupliodija i poliploidija) i strukturnih hromozomskih aberacija tipa gapova, prekida i centričnih ringova u poređenju sa negativnom kontrolom. Međutim, srednja (50 mg/kg b.w) i najviša (75 mg/kg b.w) eksperimentalna doza izazvale su signifikantno (p lt 0.001) povećanje (7.12 ± 0.26 i 9.00 ± 0.10, respektivno) Robetrsonovih (Rb) translokacija u poređenju sa negativnom kontrolom (0.00 ± 0.00). U formiranju ovih Rb translokacija utvrđeno je da učestvuju hromozomi 4 i 19. Ovi rezultati sugerišu da je dicikloheksilamin (fumagilin) ispoljio visok genotoksični potencijal sa sposobnošću indukcije i Rb. Translokacija.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo, Učestalost hromozomskih aberacija u funkciji evaluacije genotoksičnog potencijala fumagilina in vivo",
volume = "56",
number = "4",
pages = "353-366",
doi = "10.2298/AVB0604353S"
}

Stanimirović, Z., Stevanović, J., Kulić, M.,& Stojić, V.. (2006). Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(4), 353-366.
https://doi.org/10.2298/AVB0604353S

Stanimirović Z, Stevanović J, Kulić M, Stojić V. Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo. in Acta Veterinaria-Beograd. 2006;56(4):353-366.
doi:10.2298/AVB0604353S .

Stanimirović, Zoran, Stevanović, Jevrosima, Kulić, Milan, Stojić, Velibor, "Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo" in Acta Veterinaria-Beograd, 56, no. 4 (2006):353-366,
https://doi.org/10.2298/AVB0604353S . .