TY  - JOUR
AU  - Ninković, Milan
AU  - Arsić, Sveta
AU  - Žutić, Jadranka
AU  - Zdravković, Nemanja
AU  - Glišić, Dimitrije
AU  - Sapundžić Zurovac, Zorana
AU  - Bojkovski, Jovan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2578
AB  - Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.
PB  - Massimo Morgante
T2  - Large Animal Review
T1  - Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows
VL  - 27
IS  - 6
SP  - 329
EP  - 332
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2578
ER  - 

@article{
author = "Ninković, Milan and Arsić, Sveta and Žutić, Jadranka and Zdravković, Nemanja and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Bojkovski, Jovan",
year = "2021",
abstract = "Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.",
publisher = "Massimo Morgante",
journal = "Large Animal Review",
title = "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows",
volume = "27",
number = "6",
pages = "329-332",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2578"
}

Ninković, M., Arsić, S., Žutić, J., Zdravković, N., Glišić, D., Sapundžić Zurovac, Z.,& Bojkovski, J.. (2021). Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review
Massimo Morgante., 27(6), 329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578

Ninković M, Arsić S, Žutić J, Zdravković N, Glišić D, Sapundžić Zurovac Z, Bojkovski J. Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review. 2021;27(6):329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .

Ninković, Milan, Arsić, Sveta, Žutić, Jadranka, Zdravković, Nemanja, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Bojkovski, Jovan, "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows" in Large Animal Review, 27, no. 6 (2021):329-332,
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .

TY  - CONF
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Kureljušić, Branislav
AU  - Jakić-Dimić, Dobrila
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
AU  - Zdravković, Nemanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3411
AB  - Respiratory diseases represent a significant problem in pig
farming as they can cause significant economic losses all over the World. One of
the most common causes of infection is Mycoplasma hyopneumoniae (M.
hyopneumoniae) either as the primary cause of enzootic pneumonia or as one of the
causative agents of Porcine Respiratory Disease Complex (PRCD). In our study we
presented the presence of antibodies against M. hyopneumoniae in 94 blood sera of
gilts and 84 blood sera of sows, originated from 4 commercial pig farms. For this
purpose, the indirect ELISA test was used. Seroconversion was established in 11
(11.70%) sera of gilts and in 15 (17.85%) sera of sows. The percentage of positive
sera differed among the farms, ranging in gilts from 0- 28.57%, and in sows 9.09-
36.84%. The results of the study have shown that infection with M. hyopneumoniae
is present in gilts on three and in sows on all four examined farms. Successful
control of M. hyopneumoniae depends on the efficient prevention of the transfer of
the infective agent. Good results can be achieved using strict implementation of
reliable serological methods. The serological control of gilts prior to fertilization is
of extreme importance, as it can prevent spreading of the disease within the herd.
PB  - Belgrade : Institute for Animal Husbandry
C3  - 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019
T1  - Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows
SP  - 533
EP  - 540
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3411
ER  - 

@conference{
author = "Žutić, Jadranka and Valčić, Olivera and Kureljušić, Branislav and Jakić-Dimić, Dobrila and Kureljušić, Jasna and Jezdimirović, Nemanja and Zdravković, Nemanja",
year = "2019",
abstract = "Respiratory diseases represent a significant problem in pig
farming as they can cause significant economic losses all over the World. One of
the most common causes of infection is Mycoplasma hyopneumoniae (M.
hyopneumoniae) either as the primary cause of enzootic pneumonia or as one of the
causative agents of Porcine Respiratory Disease Complex (PRCD). In our study we
presented the presence of antibodies against M. hyopneumoniae in 94 blood sera of
gilts and 84 blood sera of sows, originated from 4 commercial pig farms. For this
purpose, the indirect ELISA test was used. Seroconversion was established in 11
(11.70%) sera of gilts and in 15 (17.85%) sera of sows. The percentage of positive
sera differed among the farms, ranging in gilts from 0- 28.57%, and in sows 9.09-
36.84%. The results of the study have shown that infection with M. hyopneumoniae
is present in gilts on three and in sows on all four examined farms. Successful
control of M. hyopneumoniae depends on the efficient prevention of the transfer of
the infective agent. Good results can be achieved using strict implementation of
reliable serological methods. The serological control of gilts prior to fertilization is
of extreme importance, as it can prevent spreading of the disease within the herd.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019",
title = "Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows",
pages = "533-540",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3411"
}

Žutić, J., Valčić, O., Kureljušić, B., Jakić-Dimić, D., Kureljušić, J., Jezdimirović, N.,& Zdravković, N.. (2019). Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows. in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019
Belgrade : Institute for Animal Husbandry., 533-540.
https://hdl.handle.net/21.15107/rcub_veterinar_3411

Žutić J, Valčić O, Kureljušić B, Jakić-Dimić D, Kureljušić J, Jezdimirović N, Zdravković N. Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows. in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019. 2019;:533-540.
https://hdl.handle.net/21.15107/rcub_veterinar_3411 .

Žutić, Jadranka, Valčić, Olivera, Kureljušić, Branislav, Jakić-Dimić, Dobrila, Kureljušić, Jasna, Jezdimirović, Nemanja, Zdravković, Nemanja, "Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows" in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019 (2019):533-540,
https://hdl.handle.net/21.15107/rcub_veterinar_3411 .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Aksentijević, Ksenija
AU  - Tambur, Zoran
AU  - Rakonjac, Bojan
AU  - Kovačević, Ivana
AU  - Spergser, Joachim
AU  - Loncarić, Igor
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1758
AB  - The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
VL  - 8
IS  - 1
SP  - 26
DO  - 10.3390/antibiotics8010026
ER  - 

@article{
author = "Ašanin, Jelena and Mišić, Dušan and Aksentijević, Ksenija and Tambur, Zoran and Rakonjac, Bojan and Kovačević, Ivana and Spergser, Joachim and Loncarić, Igor",
year = "2019",
abstract = "The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia",
volume = "8",
number = "1",
pages = "26",
doi = "10.3390/antibiotics8010026"
}

Ašanin, J., Mišić, D., Aksentijević, K., Tambur, Z., Rakonjac, B., Kovačević, I., Spergser, J.,& Loncarić, I.. (2019). Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel
MDPI, Basel., 8(1), 26.
https://doi.org/10.3390/antibiotics8010026

Ašanin J, Mišić D, Aksentijević K, Tambur Z, Rakonjac B, Kovačević I, Spergser J, Loncarić I. Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel. 2019;8(1):26.
doi:10.3390/antibiotics8010026 .

Ašanin, Jelena, Mišić, Dušan, Aksentijević, Ksenija, Tambur, Zoran, Rakonjac, Bojan, Kovačević, Ivana, Spergser, Joachim, Loncarić, Igor, "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia" in Antibiotics-Basel, 8, no. 1 (2019):26,
https://doi.org/10.3390/antibiotics8010026 . .

TY  - CONF
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Savić Radovanović, Radoslava
AU  - Žutić, Jadranka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2886
AB  - Проналазак антимикробних лекова сврстава се у десет највећих подухвата
људског рода XX века. На тему лечења инфекција интересовање за одабир
адекватних активних супстанци за спровођење терапије не јењава од овог
историјског почетка, а нажалост, врло брзо након увођења нових препарата
описивана је и резистенција према истим. Заправо, интересовање за
сазнањима и распрострањеношћу резистентних бактерија је повећано
увођењем система једног здравља и организованог надзора на осетљивост и
резистенцију према антимимикробним лековима. Адекватна примена лечења
и одабира лекова у терапији почиње од тумачења антибиограма.
Антибиограм представља профил антимикробне осетљивости одређеног
изолата, на основу кога се у сваком појединачном случају, врши избор
антибиотика за терапирање. Избор антибиотика за оређени изолат најчешће је
приказан у стандардима за антимикробну терапију. Најпознатији стандари
проистичу из великих комитета као што су Европски (EUCAST) или Амерички
(CLSI) а постоје и други, национални стандарди одређених држава (нпр.
Француска или Велика Британија). На основу резултата већег броја
истраживања долази се до тога који антибиотици се у датом случају могу
проценити као повољан а који као неповољан избор на основу изолата и
патолошког материјала достављеног на анализу.
Најчешће грешке при емпиријском лечењу инфекција најчешће леже у
примени неадекватног лека који не делује на узрочника инфекције или делује
незадовољавајуће. Из овог разлога је потребно скренути пажњу на адекватно
тумачење антибиограма.
AB  - The finding of antimicrobials is ranked among the ten greatest achievements of
the human race of the 20th century. Since historical beginning, the interest in
selecting adequate active substances for the implementation of infection therapy
and unfortunately, very soon after the introduction of new principles, the
resistance to them was described. In fact, the interest in the knowledge and the
prevalence of resistant bacteria has been increased by the introduction of a OneHealth system and the organized monitoring of susceptibility and resistance of
bacteria toward antimicrobial drugs. Adequate application of treatment and
selection of drugs in therapy begins with the interpretation of antibiotibigram.
Antibiogram is the profile of the antimicrobial sensitivity of a particular isolate,
based on in each individual case, antibiotic therapy is chosen. The selection of
antibiotic for particular isolate is most often shown in the standards for
antimicrobial therapy. The most well-known standards come from large
committees such as European (EUCAST) or American (CLSI), and there are other,
national standards of certain countries (e.g. France or Great Britain). Based on the
results of a large number of studies, it comes to what antibiotics can be
administered in the given case and which are an unfavorable choice based on
isolates and pathological material submitted for analysis.
The most common mistakes in the empirical treatment of infections are most
often found in the use of an inadequate drug that does not act on the cause of the
infection or its action is unsatisfactory. For this reason, it is necessary to pay
attention to the adequate interpretation of the antibiogram.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
T1  - Antibiogram u maloj veterinarskoj praksi
T1  - Antibiogram in small veterinary practice
SP  - 243
EP  - 244
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2886
ER  - 

@conference{
author = "Zdravković, Nemanja and Radanović, Oliver and Savić Radovanović, Radoslava and Žutić, Jadranka",
year = "2019",
abstract = "Проналазак антимикробних лекова сврстава се у десет највећих подухвата
људског рода XX века. На тему лечења инфекција интересовање за одабир
адекватних активних супстанци за спровођење терапије не јењава од овог
историјског почетка, а нажалост, врло брзо након увођења нових препарата
описивана је и резистенција према истим. Заправо, интересовање за
сазнањима и распрострањеношћу резистентних бактерија је повећано
увођењем система једног здравља и организованог надзора на осетљивост и
резистенцију према антимимикробним лековима. Адекватна примена лечења
и одабира лекова у терапији почиње од тумачења антибиограма.
Антибиограм представља профил антимикробне осетљивости одређеног
изолата, на основу кога се у сваком појединачном случају, врши избор
антибиотика за терапирање. Избор антибиотика за оређени изолат најчешће је
приказан у стандардима за антимикробну терапију. Најпознатији стандари
проистичу из великих комитета као што су Европски (EUCAST) или Амерички
(CLSI) а постоје и други, национални стандарди одређених држава (нпр.
Француска или Велика Британија). На основу резултата већег броја
истраживања долази се до тога који антибиотици се у датом случају могу
проценити као повољан а који као неповољан избор на основу изолата и
патолошког материјала достављеног на анализу.
Најчешће грешке при емпиријском лечењу инфекција најчешће леже у
примени неадекватног лека који не делује на узрочника инфекције или делује
незадовољавајуће. Из овог разлога је потребно скренути пажњу на адекватно
тумачење антибиограма., The finding of antimicrobials is ranked among the ten greatest achievements of
the human race of the 20th century. Since historical beginning, the interest in
selecting adequate active substances for the implementation of infection therapy
and unfortunately, very soon after the introduction of new principles, the
resistance to them was described. In fact, the interest in the knowledge and the
prevalence of resistant bacteria has been increased by the introduction of a OneHealth system and the organized monitoring of susceptibility and resistance of
bacteria toward antimicrobial drugs. Adequate application of treatment and
selection of drugs in therapy begins with the interpretation of antibiotibigram.
Antibiogram is the profile of the antimicrobial sensitivity of a particular isolate,
based on in each individual case, antibiotic therapy is chosen. The selection of
antibiotic for particular isolate is most often shown in the standards for
antimicrobial therapy. The most well-known standards come from large
committees such as European (EUCAST) or American (CLSI), and there are other,
national standards of certain countries (e.g. France or Great Britain). Based on the
results of a large number of studies, it comes to what antibiotics can be
administered in the given case and which are an unfavorable choice based on
isolates and pathological material submitted for analysis.
The most common mistakes in the empirical treatment of infections are most
often found in the use of an inadequate drug that does not act on the cause of the
infection or its action is unsatisfactory. For this reason, it is necessary to pay
attention to the adequate interpretation of the antibiogram.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.",
title = "Antibiogram u maloj veterinarskoj praksi, Antibiogram in small veterinary practice",
pages = "243-244",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2886"
}

Zdravković, N., Radanović, O., Savić Radovanović, R.,& Žutić, J.. (2019). Antibiogram u maloj veterinarskoj praksi. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
Banja Luka : Veterinarska komora Republike Srpske., 243-244.
https://hdl.handle.net/21.15107/rcub_veterinar_2886

Zdravković N, Radanović O, Savić Radovanović R, Žutić J. Antibiogram u maloj veterinarskoj praksi. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.. 2019;:243-244.
https://hdl.handle.net/21.15107/rcub_veterinar_2886 .

Zdravković, Nemanja, Radanović, Oliver, Savić Radovanović, Radoslava, Žutić, Jadranka, "Antibiogram u maloj veterinarskoj praksi" in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019. (2019):243-244,
https://hdl.handle.net/21.15107/rcub_veterinar_2886 .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://doaj.org/article/537ae23e84d24fc08ff0624c84ae15aa
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1890
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation.The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS
(CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTAsubstitution.
AB  - Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA+) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vrednosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p < 0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation.The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS
(CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTAsubstitution., Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA+) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vrednosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p < 0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata, Antibacterial effects of new endodontic materials based on calcium silicates",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - CONF
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Spalević, Ljiljana
AU  - Maslić-Strižak, Danka
AU  - Savić Radovanović, Radoslava
AU  - Kureljušić, Jasna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2885
AB  - Производња и потрошња конзумних јаја и меса живине је у сталном порасту
како у Републици Србији тако и у свету. Микробиолошко испитивање
приплодних и конзумних јаја спроводи се ради усмеравања ветеринарских и
зоотехничких мера у производњи са циљем заштите здравља животиња и људи.
Контаминација снесених јаја различитим врстама микроорганизама настаје
најчешће из спољне средине. Јаја су због свог хемијског састава добар медијум
за развој различите микрофлоре укључујући патогене микроорганизме.
Контаминација приплодних јаја гљивицама има директан утицај на
контаминацију инкубатора, развој и виталност ембриона, проценат лежења
пилића и развој болести у јатима.
До контаминација јаја долази како у објектима са живином тако и током
транспорта и лагеровања јаја. Извори гљивица у живинарском објекту су
различити: простирка, храна, ваздух, амбалажа и сл. У живинарским објектима
могуће је наћи 104-1012 спора у m3 ваздуха у зависности од начина смештаја,
старости јата густине насељености, врсте, облика (брашно, пелете) и квалитета
хране.
Материјал за рад је представљало 288 узорака прикупљених у периоду од 2016-
2017. године. Гљивице су изоловане класичним микробиолошким засејавањем
на Sabouraud агар уз идентификацију на основу морфолошких и фенотипских
особина.
Резултати су показали да је од 288 збирних узорака позитивних на испитивање
присуства гљивица највише било узорака лешева јенодневих пилића (46,96%) а
затим: јаја (15,79%), подлошки (17,77%), органа (12,96%), брисева (10,12%) и
простирки (0,40%). По врстама је најзаступљенија домаћа кокош са 93%
узорака, а затим следе фазани (7,30%), лабудови (1%) и ћурке (0,7%). Описано
је 159 изолата од којих је за 135 (84,91%) изолата извршена идентификација
само до нивоа рода Aspergillus, а 9 (5,66%) изолата је идентификовано каоA.niger, 6 (3,77%) изолата као A.flavus, 8 (5,03%) изолата као Mucor sp. и 1
(0,63%) изолат као Penicillium sp. Због могућих последица услед присуства
патогених гљивица у инкубаторским објектима, које се огледају у низу
симптома, од смрти ембриона, смањења отпорности до ширења инфекције у
ваљаонику треба интензивно примењивати зоотехничке мере, које утичу на
смањење контаминације.
AB  - The production and consumption of eggs and poultry meat is in the constant
growth both in the Republic of Sebia and in the world. The microbiological load of
breeding and table eggs is carried out in order to direct veterinary and zootechnical
measures in framing with the aim of protecting the health of animals and humans.
Contamination of the eggs originates from various types of microorganisms, and
occurs most often from the environment. Due to eggs chemical content they
represent an ideal medium for the development of different microflora including
pathogenic microorganisms. Contamination of breeding eggs with fungi has a
direct relationship with the pollution of the incubator, the development and vitality
of embryos, the percentage of laying chickens and the development of diseases in
flocks.
Contamination of eggs occurs in flock surrounding,as well, during the transport
and storage of eggs. The sources of fungi are various such as mats, food, air,
packaging, etc. In poultry farms it is possible to find up to 104-1012 spores in m3 of
air depending on the way of accommodation, age of flock, population density,
quality and type of food (powder, pellets).
Мaterial represented samples colected during the period from 2016 – 2017 year.
The fungi were isolated by classical microbiological procedure on the Sabouraud
agar followed by identification based on morphological and phenotypic
characteristics.
Our results showed that 288 group samples were positive for the presence of fungi
and the highest number of samples were one day chicks corpses (46.96%), followed
by eggs (15.79%), transport plates (17.77%), organs (12.96%), swabs (10.12%) and
floor samples (0.40%). By species, the most common were domestic chickens with
93% of the samples, followed by pheasants (7.30%), swans (1%) and turkeys
(0.7%). Of the total number of positive group samples, 159 isolates were described
and amoung them in 135 (84.91%) isolates, identification was performed only up to
the Aspergillus genus., 9 (5.66%) isolates were identified as A. niger, 6 (3.77%)isolates were identified as A. flavus, 8 (5.03%) isolates as Mucor sp. and 1 (0.63%)
isoalte as Penicillium sp. Since the consequences of the presence of pathogenic
fungi in the incubatory objects can be followed by series symptoms, from the death
of embryos, reduction in resistance to spreading infection in the roller, zootchnical
measures should be intensively applied reduceing the contamination.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
T1  - Nalaz gljivica u uzorcima živine
T1  - Occurrence of fungi in poltry samples
SP  - 69
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2885
ER  - 

@conference{
author = "Zdravković, Nemanja and Radanović, Oliver and Spalević, Ljiljana and Maslić-Strižak, Danka and Savić Radovanović, Radoslava and Kureljušić, Jasna",
year = "2018",
abstract = "Производња и потрошња конзумних јаја и меса живине је у сталном порасту
како у Републици Србији тако и у свету. Микробиолошко испитивање
приплодних и конзумних јаја спроводи се ради усмеравања ветеринарских и
зоотехничких мера у производњи са циљем заштите здравља животиња и људи.
Контаминација снесених јаја различитим врстама микроорганизама настаје
најчешће из спољне средине. Јаја су због свог хемијског састава добар медијум
за развој различите микрофлоре укључујући патогене микроорганизме.
Контаминација приплодних јаја гљивицама има директан утицај на
контаминацију инкубатора, развој и виталност ембриона, проценат лежења
пилића и развој болести у јатима.
До контаминација јаја долази како у објектима са живином тако и током
транспорта и лагеровања јаја. Извори гљивица у живинарском објекту су
различити: простирка, храна, ваздух, амбалажа и сл. У живинарским објектима
могуће је наћи 104-1012 спора у m3 ваздуха у зависности од начина смештаја,
старости јата густине насељености, врсте, облика (брашно, пелете) и квалитета
хране.
Материјал за рад је представљало 288 узорака прикупљених у периоду од 2016-
2017. године. Гљивице су изоловане класичним микробиолошким засејавањем
на Sabouraud агар уз идентификацију на основу морфолошких и фенотипских
особина.
Резултати су показали да је од 288 збирних узорака позитивних на испитивање
присуства гљивица највише било узорака лешева јенодневих пилића (46,96%) а
затим: јаја (15,79%), подлошки (17,77%), органа (12,96%), брисева (10,12%) и
простирки (0,40%). По врстама је најзаступљенија домаћа кокош са 93%
узорака, а затим следе фазани (7,30%), лабудови (1%) и ћурке (0,7%). Описано
је 159 изолата од којих је за 135 (84,91%) изолата извршена идентификација
само до нивоа рода Aspergillus, а 9 (5,66%) изолата је идентификовано каоA.niger, 6 (3,77%) изолата као A.flavus, 8 (5,03%) изолата као Mucor sp. и 1
(0,63%) изолат као Penicillium sp. Због могућих последица услед присуства
патогених гљивица у инкубаторским објектима, које се огледају у низу
симптома, од смрти ембриона, смањења отпорности до ширења инфекције у
ваљаонику треба интензивно примењивати зоотехничке мере, које утичу на
смањење контаминације., The production and consumption of eggs and poultry meat is in the constant
growth both in the Republic of Sebia and in the world. The microbiological load of
breeding and table eggs is carried out in order to direct veterinary and zootechnical
measures in framing with the aim of protecting the health of animals and humans.
Contamination of the eggs originates from various types of microorganisms, and
occurs most often from the environment. Due to eggs chemical content they
represent an ideal medium for the development of different microflora including
pathogenic microorganisms. Contamination of breeding eggs with fungi has a
direct relationship with the pollution of the incubator, the development and vitality
of embryos, the percentage of laying chickens and the development of diseases in
flocks.
Contamination of eggs occurs in flock surrounding,as well, during the transport
and storage of eggs. The sources of fungi are various such as mats, food, air,
packaging, etc. In poultry farms it is possible to find up to 104-1012 spores in m3 of
air depending on the way of accommodation, age of flock, population density,
quality and type of food (powder, pellets).
Мaterial represented samples colected during the period from 2016 – 2017 year.
The fungi were isolated by classical microbiological procedure on the Sabouraud
agar followed by identification based on morphological and phenotypic
characteristics.
Our results showed that 288 group samples were positive for the presence of fungi
and the highest number of samples were one day chicks corpses (46.96%), followed
by eggs (15.79%), transport plates (17.77%), organs (12.96%), swabs (10.12%) and
floor samples (0.40%). By species, the most common were domestic chickens with
93% of the samples, followed by pheasants (7.30%), swans (1%) and turkeys
(0.7%). Of the total number of positive group samples, 159 isolates were described
and amoung them in 135 (84.91%) isolates, identification was performed only up to
the Aspergillus genus., 9 (5.66%) isolates were identified as A. niger, 6 (3.77%)isolates were identified as A. flavus, 8 (5.03%) isolates as Mucor sp. and 1 (0.63%)
isoalte as Penicillium sp. Since the consequences of the presence of pathogenic
fungi in the incubatory objects can be followed by series symptoms, from the death
of embryos, reduction in resistance to spreading infection in the roller, zootchnical
measures should be intensively applied reduceing the contamination.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.",
title = "Nalaz gljivica u uzorcima živine, Occurrence of fungi in poltry samples",
pages = "69-72",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2885"
}

Zdravković, N., Radanović, O., Spalević, L., Maslić-Strižak, D., Savić Radovanović, R.,& Kureljušić, J.. (2018). Nalaz gljivica u uzorcima živine. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
Banja Luka : Veterinarska komora Republike Srpske., 69-72.
https://hdl.handle.net/21.15107/rcub_veterinar_2885

Zdravković N, Radanović O, Spalević L, Maslić-Strižak D, Savić Radovanović R, Kureljušić J. Nalaz gljivica u uzorcima živine. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.. 2018;:69-72.
https://hdl.handle.net/21.15107/rcub_veterinar_2885 .

Zdravković, Nemanja, Radanović, Oliver, Spalević, Ljiljana, Maslić-Strižak, Danka, Savić Radovanović, Radoslava, Kureljušić, Jasna, "Nalaz gljivica u uzorcima živine" in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018. (2018):69-72,
https://hdl.handle.net/21.15107/rcub_veterinar_2885 .

TY  - JOUR
AU  - Vojinović, Dragica
AU  - Zdravković, Nemanja
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Nedić, S.
AU  - Giadinis, Nektarios
AU  - Panousis, N.
AU  - Manić, M.
AU  - Bugarski, Dejan
AU  - Palamarević, M.
AU  - Bogićević, Nataša
AU  - Dobrosavljević, Ivan
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
AU  - Prodanov-Radulović, Jasna
AU  - Bojkovski, Jovan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1613
AB  - Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia
VL  - 69
IS  - 4
SP  - 1241
EP  - 1245
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1613
ER  - 

@article{
author = "Vojinović, Dragica and Zdravković, Nemanja and Prodanović, Radiša and Vujanac, Ivan and Nedić, S. and Giadinis, Nektarios and Panousis, N. and Manić, M. and Bugarski, Dejan and Palamarević, M. and Bogićević, Nataša and Dobrosavljević, Ivan and Spalević, Ljiljana and Žutić, Jadranka and Prodanov-Radulović, Jasna and Bojkovski, Jovan",
year = "2018",
abstract = "Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia",
volume = "69",
number = "4",
pages = "1241-1245",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1613"
}

Vojinović, D., Zdravković, N., Prodanović, R., Vujanac, I., Nedić, S., Giadinis, N., Panousis, N., Manić, M., Bugarski, D., Palamarević, M., Bogićević, N., Dobrosavljević, I., Spalević, L., Žutić, J., Prodanov-Radulović, J.,& Bojkovski, J.. (2018). Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(4), 1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613

Vojinović D, Zdravković N, Prodanović R, Vujanac I, Nedić S, Giadinis N, Panousis N, Manić M, Bugarski D, Palamarević M, Bogićević N, Dobrosavljević I, Spalević L, Žutić J, Prodanov-Radulović J, Bojkovski J. Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society. 2018;69(4):1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

Vojinović, Dragica, Zdravković, Nemanja, Prodanović, Radiša, Vujanac, Ivan, Nedić, S., Giadinis, Nektarios, Panousis, N., Manić, M., Bugarski, Dejan, Palamarević, M., Bogićević, Nataša, Dobrosavljević, Ivan, Spalević, Ljiljana, Žutić, Jadranka, Prodanov-Radulović, Jasna, Bojkovski, Jovan, "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia" in Journal of The Hellenic Veterinary Medical Society, 69, no. 4 (2018):1241-1245,
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Nišavić, Jakov
AU  - Marković, Maja
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1441
AB  - Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL.
AB  - Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibiotic resistance in bacteria isolated from fish in Serbia
T1  - Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije
VL  - 71
IS  - 1
SP  - 24
EP  - 34
DO  - 10.2298/VETGL170329005A
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan",
year = "2017",
abstract = "Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL., Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibiotic resistance in bacteria isolated from fish in Serbia, Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije",
volume = "71",
number = "1",
pages = "24-34",
doi = "10.2298/VETGL170329005A"
}

Aksentijević, K., Ašanin, J., Nišavić, J., Marković, M., Milanov, D.,& Mišić, D.. (2017). Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 24-34.
https://doi.org/10.2298/VETGL170329005A

Aksentijević K, Ašanin J, Nišavić J, Marković M, Milanov D, Mišić D. Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik. 2017;71(1):24-34.
doi:10.2298/VETGL170329005A .

Aksentijević, Ksenija, Ašanin, Jelena, Nišavić, Jakov, Marković, Maja, Milanov, Dubravka, Mišić, Dušan, "Antibiotic resistance in bacteria isolated from fish in Serbia" in Veterinarski Glasnik, 71, no. 1 (2017):24-34,
https://doi.org/10.2298/VETGL170329005A . .

TY  - JOUR
AU  - Rađenović, Milan
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1325
AB  - Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students.
AB  - Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade
T1  - Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu
VL  - 9
IS  - 2
SP  - 17
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1325
ER  - 

@article{
author = "Rađenović, Milan and Ašanin, Jelena and Aksentijević, Ksenija and Mišić, Dušan",
year = "2016",
abstract = "Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students., Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade, Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu",
volume = "9",
number = "2",
pages = "17-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1325"
}

Rađenović, M., Ašanin, J., Aksentijević, K.,& Mišić, D.. (2016). Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(2), 17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325

Rađenović M, Ašanin J, Aksentijević K, Mišić D. Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine. 2016;9(2):17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .

Rađenović, Milan, Ašanin, Jelena, Aksentijević, Ksenija, Mišić, Dušan, "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade" in Arhiv veterinarske medicine, 9, no. 2 (2016):17-28,
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Milivojević, Dušan
AU  - Čolović, Svetlana
AU  - Butorac, Ana
AU  - Cindrić, Mario
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1360
AB  - For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods.
AB  - S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method
T1  - Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode
VL  - 66
IS  - 3
SP  - 304
EP  - 316
DO  - 10.1515/acve-2016-0027
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Milivojević, Dušan and Čolović, Svetlana and Butorac, Ana and Cindrić, Mario and Mišić, Dušan",
year = "2016",
abstract = "For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods., S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method, Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode",
volume = "66",
number = "3",
pages = "304-316",
doi = "10.1515/acve-2016-0027"
}

Aksentijević, K., Ašanin, J., Milivojević, D., Čolović, S., Butorac, A., Cindrić, M.,& Mišić, D.. (2016). Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 304-316.
https://doi.org/10.1515/acve-2016-0027

Aksentijević K, Ašanin J, Milivojević D, Čolović S, Butorac A, Cindrić M, Mišić D. Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd. 2016;66(3):304-316.
doi:10.1515/acve-2016-0027 .

Aksentijević, Ksenija, Ašanin, Jelena, Milivojević, Dušan, Čolović, Svetlana, Butorac, Ana, Cindrić, Mario, Mišić, Dušan, "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method" in Acta Veterinaria-Beograd, 66, no. 3 (2016):304-316,
https://doi.org/10.1515/acve-2016-0027 . .

TY  - JOUR
AU  - Krsmanović, M.
AU  - Đoković, Radojica
AU  - Giadinis, Nektarios
AU  - Panousis, N.
AU  - Bojkovski, D.
AU  - Savić-Stevanović, Vera
AU  - Vasić, Ana
AU  - Zdravković, Nemanja
AU  - Korica, S.
AU  - Bojkovski, Jovan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1254
AB  - The results of inorganic blood parameters are presented in the periparturient period and during lactation of Simmental dairy cows. Serum calcium and inorganic phosphorus in blood of dairy cows in puerperium were significantly lower (p<0.05) compared to the values in the blood of dairy cows in advanced pregnancy and during peak of lactation, probably indicating the increased use of these macro-elements by the Simmental cows mammary gland at the early stages of lactation. When the cows were in the periparturient period serum magnesium levels were significantly lower (p<0.05) compared to the values of dairy cows during their maximal lactation probably indicating the increased use of magnesium by the Simmental dairy cow during the periparturient period.
PB  - Israel Veterinary Medical  Assoc, Raanana
T2  - Israel Journal of Veterinary Medicine
T1  - Determination of Macroelement Parameters in Different Productive Stages of Simmental Cows
VL  - 70
IS  - 1
SP  - 12
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1254
ER  - 

@article{
author = "Krsmanović, M. and Đoković, Radojica and Giadinis, Nektarios and Panousis, N. and Bojkovski, D. and Savić-Stevanović, Vera and Vasić, Ana and Zdravković, Nemanja and Korica, S. and Bojkovski, Jovan",
year = "2015",
abstract = "The results of inorganic blood parameters are presented in the periparturient period and during lactation of Simmental dairy cows. Serum calcium and inorganic phosphorus in blood of dairy cows in puerperium were significantly lower (p<0.05) compared to the values in the blood of dairy cows in advanced pregnancy and during peak of lactation, probably indicating the increased use of these macro-elements by the Simmental cows mammary gland at the early stages of lactation. When the cows were in the periparturient period serum magnesium levels were significantly lower (p<0.05) compared to the values of dairy cows during their maximal lactation probably indicating the increased use of magnesium by the Simmental dairy cow during the periparturient period.",
publisher = "Israel Veterinary Medical  Assoc, Raanana",
journal = "Israel Journal of Veterinary Medicine",
title = "Determination of Macroelement Parameters in Different Productive Stages of Simmental Cows",
volume = "70",
number = "1",
pages = "12-15",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1254"
}

Krsmanović, M., Đoković, R., Giadinis, N., Panousis, N., Bojkovski, D., Savić-Stevanović, V., Vasić, A., Zdravković, N., Korica, S.,& Bojkovski, J.. (2015). Determination of Macroelement Parameters in Different Productive Stages of Simmental Cows. in Israel Journal of Veterinary Medicine
Israel Veterinary Medical  Assoc, Raanana., 70(1), 12-15.
https://hdl.handle.net/21.15107/rcub_veterinar_1254

Krsmanović M, Đoković R, Giadinis N, Panousis N, Bojkovski D, Savić-Stevanović V, Vasić A, Zdravković N, Korica S, Bojkovski J. Determination of Macroelement Parameters in Different Productive Stages of Simmental Cows. in Israel Journal of Veterinary Medicine. 2015;70(1):12-15.
https://hdl.handle.net/21.15107/rcub_veterinar_1254 .

Krsmanović, M., Đoković, Radojica, Giadinis, Nektarios, Panousis, N., Bojkovski, D., Savić-Stevanović, Vera, Vasić, Ana, Zdravković, Nemanja, Korica, S., Bojkovski, Jovan, "Determination of Macroelement Parameters in Different Productive Stages of Simmental Cows" in Israel Journal of Veterinary Medicine, 70, no. 1 (2015):12-15,
https://hdl.handle.net/21.15107/rcub_veterinar_1254 .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Žutić, Milenko
AU  - Katić, Vera
AU  - Krnjaić, Dejan
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/854
AB  - The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml.
AB  - Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution
T1  - Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu
VL  - 66
IS  - 3-4
SP  - 199
EP  - 210
DO  - 10.2298/VETGL1204199A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Žutić, Milenko and Katić, Vera and Krnjaić, Dejan and Milić, Nenad and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml., Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution, Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu",
volume = "66",
number = "3-4",
pages = "199-210",
doi = "10.2298/VETGL1204199A"
}

Ašanin, J., Aksentijević, K., Žutić, M., Katić, V., Krnjaić, D., Milić, N., Ašanin, R.,& Mišić, D.. (2012). Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3-4), 199-210.
https://doi.org/10.2298/VETGL1204199A

Ašanin J, Aksentijević K, Žutić M, Katić V, Krnjaić D, Milić N, Ašanin R, Mišić D. Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik. 2012;66(3-4):199-210.
doi:10.2298/VETGL1204199A .

Ašanin, Jelena, Aksentijević, Ksenija, Žutić, Milenko, Katić, Vera, Krnjaić, Dejan, Milić, Nenad, Ašanin, Ružica, Mišić, Dušan, "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution" in Veterinarski Glasnik, 66, no. 3-4 (2012):199-210,
https://doi.org/10.2298/VETGL1204199A . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Zdravković, Nemanja
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/850
AB  - For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains.
AB  - Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals
T1  - Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja
VL  - 62
IS  - 4
SP  - 375
EP  - 384
DO  - 10.2298/AVB1204375A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Zdravković, Nemanja and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains., Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals, Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja",
volume = "62",
number = "4",
pages = "375-384",
doi = "10.2298/AVB1204375A"
}

Ašanin, J., Aksentijević, K., Zdravković, N., Ašanin, R.,& Mišić, D.. (2012). Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 375-384.
https://doi.org/10.2298/AVB1204375A

Ašanin J, Aksentijević K, Zdravković N, Ašanin R, Mišić D. Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd. 2012;62(4):375-384.
doi:10.2298/AVB1204375A .

Ašanin, Jelena, Aksentijević, Ksenija, Zdravković, Nemanja, Ašanin, Ružica, Mišić, Dušan, "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals" in Acta Veterinaria-Beograd, 62, no. 4 (2012):375-384,
https://doi.org/10.2298/AVB1204375A . .