TY  - CONF
AU  - Valčić, Miroslav
AU  - Radojičić, Sonja
AU  - Stević, Nataša
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3196
AB  - Epizootiološke determinante predstavljaju osnov za poznavanje epizootioloških
karakteristika pojedinih zaraznih i parazitskih oboljenja, kao i poremećaje
zdravlja nezarazne i neparazitske etiologije. U osnovi, radi se o tri determiante,
a to su:
1. Prijemčiva vrsta,
2. Uzročni faktor i
3. Spoljašnji faktor.
Region Srbije u kome se nalazi Stara planina je izuzetno interesantan sa epizootiološkog
aspekta. To se naročito odnosi na površinu koju zahvata opština
Dimitrovgad, koja obuhvata delove Stare planine i urbanu sredinu, u čijoj neposrednoj
blizini se nalazi jedan od najznačajnijih puteva koji spajaju Evropu
sa Istokom kao i na bogatstvo flore i faune kakvo se retko gde sreće u regionu.
U odnosu na prijemčive vrste, radi se o regionu u kome se nalazi veći broj vrsta
domaćih životinja čija tehnologija uzgoja značajno varira. U odnosu na uzročni
faktor, preliminarna ispitivanja su dokazala da na izgled izolovan region, može
da predstavlja značajan rezervoar mikroorganizama koji mogu da izazovu
epizootije. Istovremeno, a u sadejstvu sa spoljašnjim faktorom, naročito prometom
između regiona sveta gde se još uvek pojavljuju egzotične zarazne bolesti
životinja, postoji rizik od unosa uzročnika zaraznih bolesti koje se smatraju egzotičnim
za našu zemlju i Evropu. Raznovrsnost i variranje elemenata spoljašnje sredine kao epizootiološkog faktora,
čine da je procena epizootiološke situacije u regionu Stare planine teška,
a analiza rizika od pojave epizootija relativno kompleksna.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
T1  - Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija
SP  - 271
EP  - 283
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3196
ER  - 

@conference{
author = "Valčić, Miroslav and Radojičić, Sonja and Stević, Nataša",
year = "2021",
abstract = "Epizootiološke determinante predstavljaju osnov za poznavanje epizootioloških
karakteristika pojedinih zaraznih i parazitskih oboljenja, kao i poremećaje
zdravlja nezarazne i neparazitske etiologije. U osnovi, radi se o tri determiante,
a to su:
1. Prijemčiva vrsta,
2. Uzročni faktor i
3. Spoljašnji faktor.
Region Srbije u kome se nalazi Stara planina je izuzetno interesantan sa epizootiološkog
aspekta. To se naročito odnosi na površinu koju zahvata opština
Dimitrovgad, koja obuhvata delove Stare planine i urbanu sredinu, u čijoj neposrednoj
blizini se nalazi jedan od najznačajnijih puteva koji spajaju Evropu
sa Istokom kao i na bogatstvo flore i faune kakvo se retko gde sreće u regionu.
U odnosu na prijemčive vrste, radi se o regionu u kome se nalazi veći broj vrsta
domaćih životinja čija tehnologija uzgoja značajno varira. U odnosu na uzročni
faktor, preliminarna ispitivanja su dokazala da na izgled izolovan region, može
da predstavlja značajan rezervoar mikroorganizama koji mogu da izazovu
epizootije. Istovremeno, a u sadejstvu sa spoljašnjim faktorom, naročito prometom
između regiona sveta gde se još uvek pojavljuju egzotične zarazne bolesti
životinja, postoji rizik od unosa uzročnika zaraznih bolesti koje se smatraju egzotičnim
za našu zemlju i Evropu. Raznovrsnost i variranje elemenata spoljašnje sredine kao epizootiološkog faktora,
čine da je procena epizootiološke situacije u regionu Stare planine teška,
a analiza rizika od pojave epizootija relativno kompleksna.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021",
title = "Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija",
pages = "271-283",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3196"
}

Valčić, M., Radojičić, S.,& Stević, N.. (2021). Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
Beograd : Srpsko veterinarsko društvo., 271-283.
https://hdl.handle.net/21.15107/rcub_veterinar_3196

Valčić M, Radojičić S, Stević N. Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021. 2021;:271-283.
https://hdl.handle.net/21.15107/rcub_veterinar_3196 .

Valčić, Miroslav, Radojičić, Sonja, Stević, Nataša, "Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija" in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021 (2021):271-283,
https://hdl.handle.net/21.15107/rcub_veterinar_3196 .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Hoffmann, Bernd
AU  - Dietze, Klaas
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1795
AB  - Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.
PB  - BMC, London
T2  - Virology Journal
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA
VL  - 17
IS  - 1
SP  - 28
DO  - 10.1186/s12985-020-01298-x
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Hoffmann, Bernd and Dietze, Klaas",
year = "2020",
abstract = "Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.",
publisher = "BMC, London",
journal = "Virology Journal",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA",
volume = "17",
number = "1",
pages = "28",
doi = "10.1186/s12985-020-01298-x"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Hoffmann, B.,& Dietze, K.. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal
BMC, London., 17(1), 28.
https://doi.org/10.1186/s12985-020-01298-x

Milovanović M, Milićević V, Radojičić S, Valčić M, Hoffmann B, Dietze K. Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal. 2020;17(1):28.
doi:10.1186/s12985-020-01298-x .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Hoffmann, Bernd, Dietze, Klaas, "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA" in Virology Journal, 17, no. 1 (2020):28,
https://doi.org/10.1186/s12985-020-01298-x . .

TY  - THES
AU  - Manić, Marija
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8068
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:23531/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=35545353
UR  - https://nardus.mpn.gov.rs/handle/123456789/18211
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2756
AB  - Bolest kvrgave kože (BKK) ili nodularni dermatitis (engl. Lumpy skin disease - LSD) jeekonomski važna virusna bolest goveda koja je juna meseca 2016. godine prvi put registrovanana teritoriji R. Srbije. Cilj ove doktorske disertacije bila je izolacija i identifikacija virusa BKK,sekvenciranje dela genoma izolovanog i identifikovanih virusa BKK i molekularnoepizootiološka analiza dobijenih podataka, kao i priprema i validacija novih protokola zaizvođenje real-time PCR metode za dokazivanje terenskog i vakcinalnog soja virusa i definisanjenajspecifičnijeg i najosetljivijeg protokola za dijagnostiku BKK. U okviru doktorske disertacijeurađena je epizootiološka analiza epizootije BKK na epizootiološkom području Veterinarskogspecijalističkog instituta Niš.Uzorci za ispitivanje (krv, bioptati kože i nosni brisevi) sakupljeni su od 148 goveda tokomepizootije BKK na epizootiološkom području VSI Niš. Ukupno je ispitano je 167 uzoraka od 82životinje sa kliničkim simptomima i 66 uzoraka krvi od 66 goveda bez kliničkih simptoma kojisu uzorkovani pre vakcinacije, kao i 40 uzoraka poreklom od 20 goveda kod kojih su se kliničkisimptomi pojavili nakon vakcinacije.Iz uzorka bioptata kože goveda sa ispoljenim kliničkim simptomima urađena je izolacija virusabolesti kvrgave kože. Izolovani virus (Pčinj 1) je identifikovan primenom klasičnih virusoloških(VNT) i molekularnih metoda (real-time PCR). Sekvenciranje delova genoma virusa BKK i točetiri gena: RPO30, GPCR, EEV i P32 urađeno je iz izolovanog virusa i šest uzoraka bioptatakože (pozitivnih real-time PCR) metodom po Sanger-u. Filogenetska analiza na osnovudobijenih sekvenci RPO30, GPCR, EEV i P32 gena pokazala je potpunu podudarnost uredosledu nukleotida izolovanog i šest identifikovanih virusa BKK, kao i potpunu podudarnostsa analognim sekvencama soja Serbia/Bujanovac/2016. Utvrđeno je da oba soja virusa izolovanana području R. Srbije (Serbia/Bujanovac/2016 i Pčinj 1), kao i virusi detektovani u bioptatimakože pripadaju podgrupi terenskih sojeva virusa BKK. Analizom filogenetskog stablaformiranog na osnovu poređenja celog genoma soja Serbia/Bujanovac/2016 sa celim genomima19 CaPV koji se nalaze u banci gena (GenBank) utvrđeno je da se soj Serbia/Bujanovac/2016nalazi u podgrupi sa terenskim sojevima virusa BKK, pri čemu je najveća podudarnost uredosledu nukleotida utvrđena sa sojevima izolovanim u Grčkoj, Izraelu, Ruskoj Federaciji iJužnoj Africi.Dizajniranje sekvenci prajmera i probe za novi real-time PCR protokol za detekciju terenskogsoja virusa (Terenski Niš) zasnovano je na razlikama u redosledu nukleotida na LSD008 genuizmeđu terenskih i vakcinalnih sojeva, dok je dizajniranje sekvenci prajmera i probe za novi realtimePCR protokol za detekciju vakcinalnog soja virusa (Vakcinalni Niš) zasnovano narazlikama u redosledu nukleotida na genu LSD146 terenskog i vakcinalnog soja virusa BKK.Ispitivanjem analitičke specifičnosti utvrđeno je da se protokolom Terenski Niš pored virusaBKK detektuju i virusi boginja i ovaca, ali se jasno diferenciraju terenski i vakcinalni soj virusaBKK. Real-time PCR protokol Vakcinalni Niš je specifičan samo za vakcinalne (Neethling)sojeve virusa BKK. Ispitivanjem analitičkih i dijagnostičkih performansi potvrđeno je da realtimePCR protokoli Terenski Niš i Vakcinalni Niš poseduju zadovoljavajuće performanse i moguse koristiti u dijagnostici BKK.Ispitivanje prisustva genoma virusa BKK iz uzoraka izvršeno je primenom šest protokola zaizvođenje real-time PCR metode (Bowden i sar., 2008, KV-2, Terenski Niš, KV-vac, VakcinalniNiš i komercijalnim real-time PCR) i protokolom za izvođenje nested PCR metode (Menasherowi sar., 2014)...
AB  - Registered in the territory of the Republic of Serbia in June 2016. The aim of this doctoraldissertation was the isolation and identification of LSD virus, sequencing part of the genome ofthe isolated and identified LSD virus and molecular epizootiological analysis of the obtaineddata, as well as preparation and validation of new protocols for real-time PCR method to detectfield and vaccine strain and define the most specific and sensitive protocol. An epizootiologicalanalysis of the LSD epizootic that occurred in the epizootiological area controlled by theVeterinary Specialized Institute Nis in 2016, was also conducted within this doctoraldissertation.Samples for testing (blood, skin biopsies and nasal swabs from LSD suspected or susceptiblecattle) were collected from 148 animals during the LSD epizootic in the epizootiological area ofVSI Nis. A total of 167 samples from 82 animals with clinical symptoms and 66 blood samplesfrom 66 animals without clinical symptoms that were sampled before vaccination, as well as 40samples from 20 animals in which clinical symptoms appeared after vaccination, were examined.Isolation of the Lumpy Skin Disease Virus was performed from a bovine skin biopsies thatoriginated from animals with clinical symptoms. The isolated virus (Pčinj 1) was identified usingclassical virological (VNT) and molecular diagnostic methods (real-time PCR). Sequencing ofparts of the LSDV genome, namely four genes: RPO30, GPCR, EEV and P32, was performedfrom the isolated virus and six skin biopsy samples (positive real-time PCR) by the Sangermethod. Phylogenetic analysis based on the obtained sequences of RPO30, GPCR, EEV and P32genes showed complete match in the sequence of nucleotides from isolated and six identifiedLSD viruses, as well as with analogous sequences of the Serbia/Bujanovac/2016 LSDV strain. Itwas determined that both strains of viruses isolated in the Republic of Serbia(Serbia/Bujanovac/2016 and Pčinj 1), as well as viruses detected in skin biopsies belong to thesubgroup of field strains of LSDV. Analysis of the phylogenetic tree formed on the basis ofcomparison of the whole genome of the strain Serbia/Bujanovac/2016 with the whole genomesof 19 CaPV located in the gene bank (GenBank) showed that the Serbia/Bujanovac/2016 strain isin a subgroup along with field strains of LSDV and has the highest nucleotide sequence matchwith LSDV strains isolated in Greece, Israel, the Russian Federation and South Africa.The design of primer and probe sequences for the new real-time PCR protocol for field virusstrain detection (Terenski Niš) was based on differences in the nucleotide sequence of theLSD008 gene between field and vaccine strains, while the design of primer and probe sequencesfor the new vaccine strain detection protocol (Vakcinalni Nis) was based on differences in thesequence of nucleotides on the LSD146 gene from both field and vaccine LSDV strains. Byexamining the analytical specificity, it was determined that the Terenski Niš protocol detectsGoatpox and Sheeppox viruses in addition to the LSDV, but clearly differentiates the field andvaccine strain of the LSDV. Real-time PCR protocol Vakcinalni Nis is specific only for vaccine(Neethling) strains of LSDV. Analytical and diagnostic performance testing confirmed that thereal-time PCR protocols Terenski Niš and Vakcinalni Nis have satisfactory performance and canbe used in the diagnosis of LSD.Examination for the presence of LSDV genomes from the samples was performed using sixdifferent real-time PCR protocols (Bowden et al., 2008, KV-2, Terenski Niš, KV-vac,Vakcinalni Niš and commercial real-time PCR) as well as one nested PCR protocol(Menasherow et al., 2014)...
PB  - Универзитет у Београду, Факултет ветеринарске медицине
T2  - Универзитет у Београду
T1  - Primena molekularnih metoda u dijagnostici bolesti kvrgave kože goveda na teritoriji Republike Srbije i njihov značaj u proceni epizootiološke situacije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18211
ER  - 

@phdthesis{
author = "Manić, Marija",
year = "2020",
abstract = "Bolest kvrgave kože (BKK) ili nodularni dermatitis (engl. Lumpy skin disease - LSD) jeekonomski važna virusna bolest goveda koja je juna meseca 2016. godine prvi put registrovanana teritoriji R. Srbije. Cilj ove doktorske disertacije bila je izolacija i identifikacija virusa BKK,sekvenciranje dela genoma izolovanog i identifikovanih virusa BKK i molekularnoepizootiološka analiza dobijenih podataka, kao i priprema i validacija novih protokola zaizvođenje real-time PCR metode za dokazivanje terenskog i vakcinalnog soja virusa i definisanjenajspecifičnijeg i najosetljivijeg protokola za dijagnostiku BKK. U okviru doktorske disertacijeurađena je epizootiološka analiza epizootije BKK na epizootiološkom području Veterinarskogspecijalističkog instituta Niš.Uzorci za ispitivanje (krv, bioptati kože i nosni brisevi) sakupljeni su od 148 goveda tokomepizootije BKK na epizootiološkom području VSI Niš. Ukupno je ispitano je 167 uzoraka od 82životinje sa kliničkim simptomima i 66 uzoraka krvi od 66 goveda bez kliničkih simptoma kojisu uzorkovani pre vakcinacije, kao i 40 uzoraka poreklom od 20 goveda kod kojih su se kliničkisimptomi pojavili nakon vakcinacije.Iz uzorka bioptata kože goveda sa ispoljenim kliničkim simptomima urađena je izolacija virusabolesti kvrgave kože. Izolovani virus (Pčinj 1) je identifikovan primenom klasičnih virusoloških(VNT) i molekularnih metoda (real-time PCR). Sekvenciranje delova genoma virusa BKK i točetiri gena: RPO30, GPCR, EEV i P32 urađeno je iz izolovanog virusa i šest uzoraka bioptatakože (pozitivnih real-time PCR) metodom po Sanger-u. Filogenetska analiza na osnovudobijenih sekvenci RPO30, GPCR, EEV i P32 gena pokazala je potpunu podudarnost uredosledu nukleotida izolovanog i šest identifikovanih virusa BKK, kao i potpunu podudarnostsa analognim sekvencama soja Serbia/Bujanovac/2016. Utvrđeno je da oba soja virusa izolovanana području R. Srbije (Serbia/Bujanovac/2016 i Pčinj 1), kao i virusi detektovani u bioptatimakože pripadaju podgrupi terenskih sojeva virusa BKK. Analizom filogenetskog stablaformiranog na osnovu poređenja celog genoma soja Serbia/Bujanovac/2016 sa celim genomima19 CaPV koji se nalaze u banci gena (GenBank) utvrđeno je da se soj Serbia/Bujanovac/2016nalazi u podgrupi sa terenskim sojevima virusa BKK, pri čemu je najveća podudarnost uredosledu nukleotida utvrđena sa sojevima izolovanim u Grčkoj, Izraelu, Ruskoj Federaciji iJužnoj Africi.Dizajniranje sekvenci prajmera i probe za novi real-time PCR protokol za detekciju terenskogsoja virusa (Terenski Niš) zasnovano je na razlikama u redosledu nukleotida na LSD008 genuizmeđu terenskih i vakcinalnih sojeva, dok je dizajniranje sekvenci prajmera i probe za novi realtimePCR protokol za detekciju vakcinalnog soja virusa (Vakcinalni Niš) zasnovano narazlikama u redosledu nukleotida na genu LSD146 terenskog i vakcinalnog soja virusa BKK.Ispitivanjem analitičke specifičnosti utvrđeno je da se protokolom Terenski Niš pored virusaBKK detektuju i virusi boginja i ovaca, ali se jasno diferenciraju terenski i vakcinalni soj virusaBKK. Real-time PCR protokol Vakcinalni Niš je specifičan samo za vakcinalne (Neethling)sojeve virusa BKK. Ispitivanjem analitičkih i dijagnostičkih performansi potvrđeno je da realtimePCR protokoli Terenski Niš i Vakcinalni Niš poseduju zadovoljavajuće performanse i moguse koristiti u dijagnostici BKK.Ispitivanje prisustva genoma virusa BKK iz uzoraka izvršeno je primenom šest protokola zaizvođenje real-time PCR metode (Bowden i sar., 2008, KV-2, Terenski Niš, KV-vac, VakcinalniNiš i komercijalnim real-time PCR) i protokolom za izvođenje nested PCR metode (Menasherowi sar., 2014)..., Registered in the territory of the Republic of Serbia in June 2016. The aim of this doctoraldissertation was the isolation and identification of LSD virus, sequencing part of the genome ofthe isolated and identified LSD virus and molecular epizootiological analysis of the obtaineddata, as well as preparation and validation of new protocols for real-time PCR method to detectfield and vaccine strain and define the most specific and sensitive protocol. An epizootiologicalanalysis of the LSD epizootic that occurred in the epizootiological area controlled by theVeterinary Specialized Institute Nis in 2016, was also conducted within this doctoraldissertation.Samples for testing (blood, skin biopsies and nasal swabs from LSD suspected or susceptiblecattle) were collected from 148 animals during the LSD epizootic in the epizootiological area ofVSI Nis. A total of 167 samples from 82 animals with clinical symptoms and 66 blood samplesfrom 66 animals without clinical symptoms that were sampled before vaccination, as well as 40samples from 20 animals in which clinical symptoms appeared after vaccination, were examined.Isolation of the Lumpy Skin Disease Virus was performed from a bovine skin biopsies thatoriginated from animals with clinical symptoms. The isolated virus (Pčinj 1) was identified usingclassical virological (VNT) and molecular diagnostic methods (real-time PCR). Sequencing ofparts of the LSDV genome, namely four genes: RPO30, GPCR, EEV and P32, was performedfrom the isolated virus and six skin biopsy samples (positive real-time PCR) by the Sangermethod. Phylogenetic analysis based on the obtained sequences of RPO30, GPCR, EEV and P32genes showed complete match in the sequence of nucleotides from isolated and six identifiedLSD viruses, as well as with analogous sequences of the Serbia/Bujanovac/2016 LSDV strain. Itwas determined that both strains of viruses isolated in the Republic of Serbia(Serbia/Bujanovac/2016 and Pčinj 1), as well as viruses detected in skin biopsies belong to thesubgroup of field strains of LSDV. Analysis of the phylogenetic tree formed on the basis ofcomparison of the whole genome of the strain Serbia/Bujanovac/2016 with the whole genomesof 19 CaPV located in the gene bank (GenBank) showed that the Serbia/Bujanovac/2016 strain isin a subgroup along with field strains of LSDV and has the highest nucleotide sequence matchwith LSDV strains isolated in Greece, Israel, the Russian Federation and South Africa.The design of primer and probe sequences for the new real-time PCR protocol for field virusstrain detection (Terenski Niš) was based on differences in the nucleotide sequence of theLSD008 gene between field and vaccine strains, while the design of primer and probe sequencesfor the new vaccine strain detection protocol (Vakcinalni Nis) was based on differences in thesequence of nucleotides on the LSD146 gene from both field and vaccine LSDV strains. Byexamining the analytical specificity, it was determined that the Terenski Niš protocol detectsGoatpox and Sheeppox viruses in addition to the LSDV, but clearly differentiates the field andvaccine strain of the LSDV. Real-time PCR protocol Vakcinalni Nis is specific only for vaccine(Neethling) strains of LSDV. Analytical and diagnostic performance testing confirmed that thereal-time PCR protocols Terenski Niš and Vakcinalni Nis have satisfactory performance and canbe used in the diagnosis of LSD.Examination for the presence of LSDV genomes from the samples was performed using sixdifferent real-time PCR protocols (Bowden et al., 2008, KV-2, Terenski Niš, KV-vac,Vakcinalni Niš and commercial real-time PCR) as well as one nested PCR protocol(Menasherow et al., 2014)...",
publisher = "Универзитет у Београду, Факултет ветеринарске медицине",
journal = "Универзитет у Београду",
title = "Primena molekularnih metoda u dijagnostici bolesti kvrgave kože goveda na teritoriji Republike Srbije i njihov značaj u proceni epizootiološke situacije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18211"
}

Manić, M.. (2020). Primena molekularnih metoda u dijagnostici bolesti kvrgave kože goveda na teritoriji Republike Srbije i njihov značaj u proceni epizootiološke situacije. in Универзитет у Београду
Универзитет у Београду, Факултет ветеринарске медицине..
https://hdl.handle.net/21.15107/rcub_nardus_18211

Manić M. Primena molekularnih metoda u dijagnostici bolesti kvrgave kože goveda na teritoriji Republike Srbije i njihov značaj u proceni epizootiološke situacije. in Универзитет у Београду. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_18211 .

Manić, Marija, "Primena molekularnih metoda u dijagnostici bolesti kvrgave kože goveda na teritoriji Republike Srbije i njihov značaj u proceni epizootiološke situacije" in Универзитет у Београду (2020),
https://hdl.handle.net/21.15107/rcub_nardus_18211 .

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
AU  - Potkonjak, Aleksandar
AU  - Stojanac, Nenad
AU  - Kureljušić, Branislav
AU  - Veljović, Lubiša
AU  - Radosavljević, Vladimir
AU  - Savić, Božidar
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1987
AB  - Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage.
AB  - Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018
T1  - Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine
VL  - 70
IS  - 1
SP  - 110
EP  - 125
DO  - 10.2478/acve-2020-0008
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Stevančević, Ognjen and Chiapponi, Chiara and Potkonjak, Aleksandar and Stojanac, Nenad and Kureljušić, Branislav and Veljović, Lubiša and Radosavljević, Vladimir and Savić, Božidar",
year = "2020",
abstract = "Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage., Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.",
publisher = "Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018, Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine",
volume = "70",
number = "1",
pages = "110-125",
doi = "10.2478/acve-2020-0008"
}

Maksimović Zorić, J., Milićević, V., Stevančević, O., Chiapponi, C., Potkonjak, A., Stojanac, N., Kureljušić, B., Veljović, L., Radosavljević, V.,& Savić, B.. (2020). Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd
Sciendo., 70(1), 110-125.
https://doi.org/10.2478/acve-2020-0008

Maksimović Zorić J, Milićević V, Stevančević O, Chiapponi C, Potkonjak A, Stojanac N, Kureljušić B, Veljović L, Radosavljević V, Savić B. Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd. 2020;70(1):110-125.
doi:10.2478/acve-2020-0008 .

Maksimović Zorić, Jelena, Milićević, Vesna, Stevančević, Ognjen, Chiapponi, Chiara, Potkonjak, Aleksandar, Stojanac, Nenad, Kureljušić, Branislav, Veljović, Lubiša, Radosavljević, Vladimir, Savić, Božidar, "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018" in Acta Veterinaria-Beograd, 70, no. 1 (2020):110-125,
https://doi.org/10.2478/acve-2020-0008 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Nišavić, Jakov
AU  - Radojičić, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1781
AB  - Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia
VL  - 39
IS  - 3
SP  - 433
EP  - 437
DO  - 10.29261/pakvetj/2019.034
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Valčić, Miroslav and Stević, Nataša and Nišavić, Jakov and Radojičić, Sonja",
year = "2019",
abstract = "Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia",
volume = "39",
number = "3",
pages = "433-437",
doi = "10.29261/pakvetj/2019.034"
}

Milovanović, M., Milićević, V., Valčić, M., Stević, N., Nišavić, J.,& Radojičić, S.. (2019). Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 39(3), 433-437.
https://doi.org/10.29261/pakvetj/2019.034

Milovanović M, Milićević V, Valčić M, Stević N, Nišavić J, Radojičić S. Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal. 2019;39(3):433-437.
doi:10.29261/pakvetj/2019.034 .

Milovanović, Milovan, Milićević, Vesna, Valčić, Miroslav, Stević, Nataša, Nišavić, Jakov, Radojičić, Sonja, "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia" in Pakistan Veterinary Journal, 39, no. 3 (2019):433-437,
https://doi.org/10.29261/pakvetj/2019.034 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Dietze, Klaas
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Moritz, Tom
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1732
AB  - BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
VL  - 15
SP  - 80
DO  - 10.1186/s12917-019-1831-y
ER  - 

@article{
author = "Milovanović, Milovan and Dietze, Klaas and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Moritz, Tom and Hoffmann, Bernd",
year = "2019",
abstract = "BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests",
volume = "15",
pages = "80",
doi = "10.1186/s12917-019-1831-y"
}

Milovanović, M., Dietze, K., Milićević, V., Radojičić, S., Valčić, M., Moritz, T.,& Hoffmann, B.. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research
BMC, London., 15, 80.
https://doi.org/10.1186/s12917-019-1831-y

Milovanović M, Dietze K, Milićević V, Radojičić S, Valčić M, Moritz T, Hoffmann B. Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research. 2019;15:80.
doi:10.1186/s12917-019-1831-y .

Milovanović, Milovan, Dietze, Klaas, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Moritz, Tom, Hoffmann, Bernd, "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests" in BMC Veterinary Research, 15 (2019):80,
https://doi.org/10.1186/s12917-019-1831-y . .

TY  - JOUR
AU  - Vojinović, Dragica
AU  - Zdravković, Nemanja
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Nedić, S.
AU  - Giadinis, Nektarios
AU  - Panousis, N.
AU  - Manić, M.
AU  - Bugarski, Dejan
AU  - Palamarević, M.
AU  - Bogićević, Nataša
AU  - Dobrosavljević, Ivan
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
AU  - Prodanov-Radulović, Jasna
AU  - Bojkovski, Jovan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1613
AB  - Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia
VL  - 69
IS  - 4
SP  - 1241
EP  - 1245
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1613
ER  - 

@article{
author = "Vojinović, Dragica and Zdravković, Nemanja and Prodanović, Radiša and Vujanac, Ivan and Nedić, S. and Giadinis, Nektarios and Panousis, N. and Manić, M. and Bugarski, Dejan and Palamarević, M. and Bogićević, Nataša and Dobrosavljević, Ivan and Spalević, Ljiljana and Žutić, Jadranka and Prodanov-Radulović, Jasna and Bojkovski, Jovan",
year = "2018",
abstract = "Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia",
volume = "69",
number = "4",
pages = "1241-1245",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1613"
}

Vojinović, D., Zdravković, N., Prodanović, R., Vujanac, I., Nedić, S., Giadinis, N., Panousis, N., Manić, M., Bugarski, D., Palamarević, M., Bogićević, N., Dobrosavljević, I., Spalević, L., Žutić, J., Prodanov-Radulović, J.,& Bojkovski, J.. (2018). Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(4), 1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613

Vojinović D, Zdravković N, Prodanović R, Vujanac I, Nedić S, Giadinis N, Panousis N, Manić M, Bugarski D, Palamarević M, Bogićević N, Dobrosavljević I, Spalević L, Žutić J, Prodanov-Radulović J, Bojkovski J. Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society. 2018;69(4):1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

Vojinović, Dragica, Zdravković, Nemanja, Prodanović, Radiša, Vujanac, Ivan, Nedić, S., Giadinis, Nektarios, Panousis, N., Manić, M., Bugarski, Dejan, Palamarević, M., Bogićević, Nataša, Dobrosavljević, Ivan, Spalević, Ljiljana, Žutić, Jadranka, Prodanov-Radulović, Jasna, Bojkovski, Jovan, "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia" in Journal of The Hellenic Veterinary Medical Society, 69, no. 4 (2018):1241-1245,
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

TY  - JOUR
AU  - Stević, Nataša
AU  - Milovanović, Milovan
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2155
AB  - Kada se bruceloza ustanovi u jednoj zemlji, međunarodni veterinarski propisi
nameću ograničenja kretanja životinja i trgovine, što dovodi do velikih ekonomskih
gubitaka. Izolacija Brucella spp. konvencionalnim bakteriološkim tehnikama
je dugotrajna, rizična za laboratorijske radnike i niske osetljivosti zbog česte
kontaminacije materijala. Negativna izolacija ne isključuje postojanje bruceloze.
Najbolji rezultati do sada su dobijeni kombinovanjem metoda izolacije i PCR metode
na kliničkim uzorcima. Nedostatak PCR metoda baziranih na razlici među sojevima
unutar vrste stimulisao je razvoj novih tehnika „otisaka prstiju”. Podaci o sekvenci
celog genoma brucela omogućili su identifikaciju i razlikovanje brucela na nivou vrste,
biovara i upoređivanje sojeva što je olakšalo pronalaženje izvora infekcije. Indirektni
dijagnostički testovi su zasnovani na detekciji imunskog odgovora izazvanog
infekcijom. Ovi testovi pokazuju različit stepen osetljivosti i specifičnosti zavisno od
brojnih varijabli, poput stepena i načina inficiranja, prisustva takozvanih „unakrsno
reaktivnih bakterija” antigenski sličnih Brucella spp, kinetike indukovanog imunskog
odgovora i prethodne vakcinacije. Imajući u vidu kinetiku nastajanja imunskog
odgovora indukovanog nakon infekcije, vreme kada će se različiti testovi izvoditi ima
veliki uticaj na rezultate.
AB  - When brucellosis is detected in a country, international veterinary regulations impose
restrictions on animal movements and trade, which result in huge economic losses.
The isolation of Brucella spp. through conventional bacteriological techniques takes
a long time, it’s risky for the laboratory workers, and has low sensitivity due to
the frequent contamination of materials. Negative cultures do not rule out the disease.
The best results have so far been obtained by combining culture and PCR detection on
clinical samples. The lack of PCR-based methods for differentiation among strains within
a species stimulate the development of new techniques-”fingerprinting methods”.
Data on the sequence of the genome of Brucella enabled the identification and differentiation
of Brucella at the level of species and biovar and comparison strains which
is easier to find the source of infection. Indirect diagnostic tests are based on the detection
of immune responses induced by infection. These tests show different sensitivities
and specificities depending on numerous variables, such as the infection dose and
route, the presence of so-called “cross-reactive bacteria” antigenically similar to Brucella
spp., the kinetics of the induced immune response, and previous vaccination. Bearing
in mind kinetics of the immune response induced after infection, the time when
the different tests carried out has a major impact on the results.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Mogućnost dijagnostike bruceloze domaćih životinja
T1  - The possibility of diagnosis of domestic animal Brucellosis
VL  - 17
IS  - 1
SP  - 93
EP  - 104
DO  - 10.7251/VETJ1701093S
ER  - 

@article{
author = "Stević, Nataša and Milovanović, Milovan and Radojičić, Sonja and Valčić, Miroslav",
year = "2017",
abstract = "Kada se bruceloza ustanovi u jednoj zemlji, međunarodni veterinarski propisi
nameću ograničenja kretanja životinja i trgovine, što dovodi do velikih ekonomskih
gubitaka. Izolacija Brucella spp. konvencionalnim bakteriološkim tehnikama
je dugotrajna, rizična za laboratorijske radnike i niske osetljivosti zbog česte
kontaminacije materijala. Negativna izolacija ne isključuje postojanje bruceloze.
Najbolji rezultati do sada su dobijeni kombinovanjem metoda izolacije i PCR metode
na kliničkim uzorcima. Nedostatak PCR metoda baziranih na razlici među sojevima
unutar vrste stimulisao je razvoj novih tehnika „otisaka prstiju”. Podaci o sekvenci
celog genoma brucela omogućili su identifikaciju i razlikovanje brucela na nivou vrste,
biovara i upoređivanje sojeva što je olakšalo pronalaženje izvora infekcije. Indirektni
dijagnostički testovi su zasnovani na detekciji imunskog odgovora izazvanog
infekcijom. Ovi testovi pokazuju različit stepen osetljivosti i specifičnosti zavisno od
brojnih varijabli, poput stepena i načina inficiranja, prisustva takozvanih „unakrsno
reaktivnih bakterija” antigenski sličnih Brucella spp, kinetike indukovanog imunskog
odgovora i prethodne vakcinacije. Imajući u vidu kinetiku nastajanja imunskog
odgovora indukovanog nakon infekcije, vreme kada će se različiti testovi izvoditi ima
veliki uticaj na rezultate., When brucellosis is detected in a country, international veterinary regulations impose
restrictions on animal movements and trade, which result in huge economic losses.
The isolation of Brucella spp. through conventional bacteriological techniques takes
a long time, it’s risky for the laboratory workers, and has low sensitivity due to
the frequent contamination of materials. Negative cultures do not rule out the disease.
The best results have so far been obtained by combining culture and PCR detection on
clinical samples. The lack of PCR-based methods for differentiation among strains within
a species stimulate the development of new techniques-”fingerprinting methods”.
Data on the sequence of the genome of Brucella enabled the identification and differentiation
of Brucella at the level of species and biovar and comparison strains which
is easier to find the source of infection. Indirect diagnostic tests are based on the detection
of immune responses induced by infection. These tests show different sensitivities
and specificities depending on numerous variables, such as the infection dose and
route, the presence of so-called “cross-reactive bacteria” antigenically similar to Brucella
spp., the kinetics of the induced immune response, and previous vaccination. Bearing
in mind kinetics of the immune response induced after infection, the time when
the different tests carried out has a major impact on the results.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Mogućnost dijagnostike bruceloze domaćih životinja, The possibility of diagnosis of domestic animal Brucellosis",
volume = "17",
number = "1",
pages = "93-104",
doi = "10.7251/VETJ1701093S"
}

Stević, N., Milovanović, M., Radojičić, S.,& Valčić, M.. (2017). Mogućnost dijagnostike bruceloze domaćih životinja. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(1), 93-104.
https://doi.org/10.7251/VETJ1701093S

Stević N, Milovanović M, Radojičić S, Valčić M. Mogućnost dijagnostike bruceloze domaćih životinja. in Veterinarski žurnal Republike Srpske. 2017;17(1):93-104.
doi:10.7251/VETJ1701093S .

Stević, Nataša, Milovanović, Milovan, Radojičić, Sonja, Valčić, Miroslav, "Mogućnost dijagnostike bruceloze domaćih životinja" in Veterinarski žurnal Republike Srpske, 17, no. 1 (2017):93-104,
https://doi.org/10.7251/VETJ1701093S . .

TY  - JOUR
AU  - Zorić-Maksimović, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Pavlović, Ivan
AU  - Radosavljević, Vladimir
AU  - Valčić, Miroslav
AU  - Glišić, Mileva
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1329
AB  - Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus.
AB  - Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis
T1  - Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika
VL  - 9
IS  - 1
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1329
ER  - 

@article{
author = "Zorić-Maksimović, Jelena and Milićević, Vesna and Veljović, Ljubiša and Pavlović, Ivan and Radosavljević, Vladimir and Valčić, Miroslav and Glišić, Mileva",
year = "2016",
abstract = "Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus., Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis, Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika",
volume = "9",
number = "1",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1329"
}

Zorić-Maksimović, J., Milićević, V., Veljović, L., Pavlović, I., Radosavljević, V., Valčić, M.,& Glišić, M.. (2016). Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(1), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329

Zorić-Maksimović J, Milićević V, Veljović L, Pavlović I, Radosavljević V, Valčić M, Glišić M. Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine. 2016;9(1):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

Zorić-Maksimović, Jelena, Milićević, Vesna, Veljović, Ljubiša, Pavlović, Ivan, Radosavljević, Vladimir, Valčić, Miroslav, Glišić, Mileva, "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis" in Arhiv veterinarske medicine, 9, no. 1 (2016):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

TY  - JOUR
AU  - Stanojević, S.
AU  - Valčić, Miroslav
AU  - Stanojević, S.
AU  - Radojičić, Sonja
AU  - Avramov, S.
AU  - Tambur, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1280
AB  - Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epidemic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in the protection and surveillance zone proved to be the most effective measures to stop the spread and for the eradication of the disease. Moreover, during the simulation, systematic implementation of biosecurity measures in all pig production clusters was demonstrated to be an appropriate strategy for sustainable control of CSF and the establishment of a stable epidemiological situation.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Veterinarni Medicina
T1  - Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia
VL  - 60
IS  - 10
DO  - 10.17221/8494-VETMED
ER  - 

@article{
author = "Stanojević, S. and Valčić, Miroslav and Stanojević, S. and Radojičić, Sonja and Avramov, S. and Tambur, Zoran",
year = "2015",
abstract = "Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epidemic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in the protection and surveillance zone proved to be the most effective measures to stop the spread and for the eradication of the disease. Moreover, during the simulation, systematic implementation of biosecurity measures in all pig production clusters was demonstrated to be an appropriate strategy for sustainable control of CSF and the establishment of a stable epidemiological situation.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Veterinarni Medicina",
title = "Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia",
volume = "60",
number = "10",
doi = "10.17221/8494-VETMED"
}

Stanojević, S., Valčić, M., Stanojević, S., Radojičić, S., Avramov, S.,& Tambur, Z.. (2015). Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia. in Veterinarni Medicina
Czech Academy Agricultural Sciences, Prague., 60(10).
https://doi.org/10.17221/8494-VETMED

Stanojević S, Valčić M, Stanojević S, Radojičić S, Avramov S, Tambur Z. Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia. in Veterinarni Medicina. 2015;60(10).
doi:10.17221/8494-VETMED .

Stanojević, S., Valčić, Miroslav, Stanojević, S., Radojičić, Sonja, Avramov, S., Tambur, Zoran, "Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia" in Veterinarni Medicina, 60, no. 10 (2015),
https://doi.org/10.17221/8494-VETMED . .

TY  - JOUR
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Polaček, Vladimir
AU  - Valčić, Miroslav
AU  - Lazić, Gospava
AU  - Pasagić, Enisa
AU  - Petrović, Tamaš
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1180
AB  - The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of equine arteritis virus in the semen of stallions in the republic of Serbia
VL  - 65
IS  - 4
SP  - 557
EP  - 567
DO  - 10.1515/acve-2015-0047
ER  - 

@article{
author = "Lazić, Sava and Lupulović, Diana and Polaček, Vladimir and Valčić, Miroslav and Lazić, Gospava and Pasagić, Enisa and Petrović, Tamaš",
year = "2015",
abstract = "The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia",
volume = "65",
number = "4",
pages = "557-567",
doi = "10.1515/acve-2015-0047"
}

Lazić, S., Lupulović, D., Polaček, V., Valčić, M., Lazić, G., Pasagić, E.,& Petrović, T.. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 557-567.
https://doi.org/10.1515/acve-2015-0047

Lazić S, Lupulović D, Polaček V, Valčić M, Lazić G, Pasagić E, Petrović T. Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):557-567.
doi:10.1515/acve-2015-0047 .

Lazić, Sava, Lupulović, Diana, Polaček, Vladimir, Valčić, Miroslav, Lazić, Gospava, Pasagić, Enisa, Petrović, Tamaš, "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):557-567,
https://doi.org/10.1515/acve-2015-0047 . .

TY  - JOUR
AU  - Vojinović, Dragica
AU  - Bogićević, Nataša
AU  - Vasić, Ana
AU  - Manić, Marija
AU  - Elezović-Radovanović, Milica
AU  - Rogožarski, Dragan
AU  - Marić, Jovan
AU  - Valčić, Miroslav
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1285
AB  - Leptospirosis is a bacterial zoonosis with worldwide distribution. This disease is facilitated among stray dogs due to their lifestyle and the absence of immunoprophylaxis. The aim of the present study was to provide serological data on the presence of certain serovars of Leptospira spp., which are assumed to circulate in the population of stray dogs in Serbia. During a period of 3 years (from April 2010 to June 2013), 1045 canine sera originating from 11 shelters were submitted to the laboratory of the Department of Infectious Animal Diseases and Diseases of Bees, Faculty of Veterinary Medicine, Belgrade. A microscopic agglutination test (MAT) was performed to detect antibodies to Leptospira (cutoff, 1: 100). The overall seroprevalence was 5.45% (57/1045) and the most prevalent Leptospira serovars were Icterohaemorrhagiae 33.3% (19/57), Pomona 29.8% (17/57), Canicola 14.0% (8/57), Grippotyphosa 3.5% (2/57), Bataviae 1.7 % (1/57), and Sejroe 1.7% (1/57). All dogs were seronegative for antibodies against serovars Australis and Bratislava. The results showed that stray dogs contribute to the spread and maintenance of Leptospira spp. in Serbia. Due to close contact with humans it is very important to improve the prevention of leptospirosis in dogs and support a One Health approach.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - Seroepidemiological survey of leptospiral infection in stray dogs in Serbia
VL  - 39
IS  - 6
SP  - 719
EP  - 723
DO  - 10.3906/vet-1505-18
ER  - 

@article{
author = "Vojinović, Dragica and Bogićević, Nataša and Vasić, Ana and Manić, Marija and Elezović-Radovanović, Milica and Rogožarski, Dragan and Marić, Jovan and Valčić, Miroslav",
year = "2015",
abstract = "Leptospirosis is a bacterial zoonosis with worldwide distribution. This disease is facilitated among stray dogs due to their lifestyle and the absence of immunoprophylaxis. The aim of the present study was to provide serological data on the presence of certain serovars of Leptospira spp., which are assumed to circulate in the population of stray dogs in Serbia. During a period of 3 years (from April 2010 to June 2013), 1045 canine sera originating from 11 shelters were submitted to the laboratory of the Department of Infectious Animal Diseases and Diseases of Bees, Faculty of Veterinary Medicine, Belgrade. A microscopic agglutination test (MAT) was performed to detect antibodies to Leptospira (cutoff, 1: 100). The overall seroprevalence was 5.45% (57/1045) and the most prevalent Leptospira serovars were Icterohaemorrhagiae 33.3% (19/57), Pomona 29.8% (17/57), Canicola 14.0% (8/57), Grippotyphosa 3.5% (2/57), Bataviae 1.7 % (1/57), and Sejroe 1.7% (1/57). All dogs were seronegative for antibodies against serovars Australis and Bratislava. The results showed that stray dogs contribute to the spread and maintenance of Leptospira spp. in Serbia. Due to close contact with humans it is very important to improve the prevention of leptospirosis in dogs and support a One Health approach.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "Seroepidemiological survey of leptospiral infection in stray dogs in Serbia",
volume = "39",
number = "6",
pages = "719-723",
doi = "10.3906/vet-1505-18"
}

Vojinović, D., Bogićević, N., Vasić, A., Manić, M., Elezović-Radovanović, M., Rogožarski, D., Marić, J.,& Valčić, M.. (2015). Seroepidemiological survey of leptospiral infection in stray dogs in Serbia. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 39(6), 719-723.
https://doi.org/10.3906/vet-1505-18

Vojinović D, Bogićević N, Vasić A, Manić M, Elezović-Radovanović M, Rogožarski D, Marić J, Valčić M. Seroepidemiological survey of leptospiral infection in stray dogs in Serbia. in Turkish Journal of Veterinary & Animal Sciences. 2015;39(6):719-723.
doi:10.3906/vet-1505-18 .

Vojinović, Dragica, Bogićević, Nataša, Vasić, Ana, Manić, Marija, Elezović-Radovanović, Milica, Rogožarski, Dragan, Marić, Jovan, Valčić, Miroslav, "Seroepidemiological survey of leptospiral infection in stray dogs in Serbia" in Turkish Journal of Veterinary & Animal Sciences, 39, no. 6 (2015):719-723,
https://doi.org/10.3906/vet-1505-18 . .

TY  - JOUR
AU  - Obrenović, Sonja
AU  - Radojičić, Sonja
AU  - Stević, Nataša
AU  - Bogunović, Danica
AU  - Vakanjac, Slobodanka
AU  - Valčić, Miroslav
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1160
AB  - With its epizootiological characteristics, the territory of the municipality of Belgrade city is a potentially important locality for the maintenance and spreading of a number of serovars of Leptospira interrogans. In order to evaluate the epizootiological situation as far as animal leptospirosis in the Belgrade region is concerned, from January 2012 until June 2013 the prevalence of cat leptospirosis has been evaluated. The standard microagglutination test (MAT) was used to determine animals sero positive to different serovars that belong to L. interrogans sensu lato complex. The antigens used were: Icterohaemorrhagiae, Grippotyphosa, Pomona, Canicola, Bratislava, Batavie, Sejroe, Pyrogenes, Australis and Autumnalis. Out of the total number of tested animals, there were 43 (26.7%) positive to one, two or three serovar(s). Out of a total of 43 positive sera 20 (46.5%) samples were positive to more than one leptospira serovar.
AB  - Svojim epizootiološkim karakteristikama, teritorija grada Beograda je potencijalno značajna lokacija za održavanje i širenje brojnih serovarijeteta vrste Leptospira interrogans. U cilju evaluacije epizootiološke situacije u odnosu na leptospirozu kod vrsta životinja na teritoriji grada Beograda, obavljena su ispitivanja seroprevalencije leptospiroze mačaka u periodu od januara 2012. godine, do juna 2013. godine. Radi utvrđivanja prisustva antitela na različite serovarijetete leptospira, kao dijagnostički metod, upotrebljen je standardni mikroaglutinacioni test (MAT). U okviru navedene dijagnostičke metode, upotrebljeni su različiti serovarijeteti bakterije L. interrogans sensu lato kompleksa i to: sv. Icterohaemorrhagiae, sv. Grippotyphosa, sv. Pomona, sv. Canicola, sv. Bratislava, sv. Batavie, sv. Sejroe, sv. Pyrogenes, sv. Australis i sv. Autumnalis. Od ukupnog broja ispitivanih životinja, pozitivnih je bilo 43 (26.7%) i to jedan, dva i/ili tri serovarijeteta. Od ukupnog broja seropozitivnih životinja, 20 seruma (46.5%) je bilo seropozitivno na više od jedan serovarijetet leptospira.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Seroprevalence of cat leptospirosis in Belgrade: Serbia
T1  - Seroprevalencija leptospiroze mačaka na teritoriji Beograda - Srbija
VL  - 64
IS  - 4
SP  - 510
EP  - 518
DO  - 10.2478/acve-2014-0047
ER  - 

@article{
author = "Obrenović, Sonja and Radojičić, Sonja and Stević, Nataša and Bogunović, Danica and Vakanjac, Slobodanka and Valčić, Miroslav",
year = "2014",
abstract = "With its epizootiological characteristics, the territory of the municipality of Belgrade city is a potentially important locality for the maintenance and spreading of a number of serovars of Leptospira interrogans. In order to evaluate the epizootiological situation as far as animal leptospirosis in the Belgrade region is concerned, from January 2012 until June 2013 the prevalence of cat leptospirosis has been evaluated. The standard microagglutination test (MAT) was used to determine animals sero positive to different serovars that belong to L. interrogans sensu lato complex. The antigens used were: Icterohaemorrhagiae, Grippotyphosa, Pomona, Canicola, Bratislava, Batavie, Sejroe, Pyrogenes, Australis and Autumnalis. Out of the total number of tested animals, there were 43 (26.7%) positive to one, two or three serovar(s). Out of a total of 43 positive sera 20 (46.5%) samples were positive to more than one leptospira serovar., Svojim epizootiološkim karakteristikama, teritorija grada Beograda je potencijalno značajna lokacija za održavanje i širenje brojnih serovarijeteta vrste Leptospira interrogans. U cilju evaluacije epizootiološke situacije u odnosu na leptospirozu kod vrsta životinja na teritoriji grada Beograda, obavljena su ispitivanja seroprevalencije leptospiroze mačaka u periodu od januara 2012. godine, do juna 2013. godine. Radi utvrđivanja prisustva antitela na različite serovarijetete leptospira, kao dijagnostički metod, upotrebljen je standardni mikroaglutinacioni test (MAT). U okviru navedene dijagnostičke metode, upotrebljeni su različiti serovarijeteti bakterije L. interrogans sensu lato kompleksa i to: sv. Icterohaemorrhagiae, sv. Grippotyphosa, sv. Pomona, sv. Canicola, sv. Bratislava, sv. Batavie, sv. Sejroe, sv. Pyrogenes, sv. Australis i sv. Autumnalis. Od ukupnog broja ispitivanih životinja, pozitivnih je bilo 43 (26.7%) i to jedan, dva i/ili tri serovarijeteta. Od ukupnog broja seropozitivnih životinja, 20 seruma (46.5%) je bilo seropozitivno na više od jedan serovarijetet leptospira.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Seroprevalence of cat leptospirosis in Belgrade: Serbia, Seroprevalencija leptospiroze mačaka na teritoriji Beograda - Srbija",
volume = "64",
number = "4",
pages = "510-518",
doi = "10.2478/acve-2014-0047"
}

Obrenović, S., Radojičić, S., Stević, N., Bogunović, D., Vakanjac, S.,& Valčić, M.. (2014). Seroprevalence of cat leptospirosis in Belgrade: Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(4), 510-518.
https://doi.org/10.2478/acve-2014-0047

Obrenović S, Radojičić S, Stević N, Bogunović D, Vakanjac S, Valčić M. Seroprevalence of cat leptospirosis in Belgrade: Serbia. in Acta Veterinaria-Beograd. 2014;64(4):510-518.
doi:10.2478/acve-2014-0047 .

Obrenović, Sonja, Radojičić, Sonja, Stević, Nataša, Bogunović, Danica, Vakanjac, Slobodanka, Valčić, Miroslav, "Seroprevalence of cat leptospirosis in Belgrade: Serbia" in Acta Veterinaria-Beograd, 64, no. 4 (2014):510-518,
https://doi.org/10.2478/acve-2014-0047 . .

TY  - JOUR
AU  - Manić, Marija
AU  - Prokić, Nataša
AU  - Gojković, Katarina
AU  - Đorić, Goran
AU  - Vasić, Ana
AU  - Marić, Jovan
AU  - Vojinović, Dragica
AU  - Đuričić, Bosiljka
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1120
AB  - Since the last decade, stray dogs have been significant ecological, economical and sociological problem in the world as well as in the Republic of Serbia. They occupy specifi c place in the ecology of big cities and their contact with human population represents danger since they are carriers of many zoonotic infectious diseases. The goal of this paper is to present epizootiological survey on some diseases of bacterial, viral and parasitic origin through serological investigation of blood sera and full blood. The prevalence of leptospirosis, Tularaemia, West Nile fever and dirofilariosis has been determined in this research. The material included full blood and blood sera of stray dogs in the wider territory of Leskovac city. The research methods encompassed standard serological tests: microscopic agglutination test (MAT) for determination of specific antibodies against Leptospira spp.; slow and fast agglutination test for determination of antibodies against Tularaemia causative agent (Francisella tularensis); agar gel immunodiffusion (AGID) for detection of specific antibodies against West Nile virus -WNV; modified Knot's test for the detection of microfilaria. The results revealed seropositivity for causative agents of Leptospirosis, Tularaemia and dirofilariosis, while there were no positive findings for WNV.
AB  - Tokom poslednjih deset godina, psi lutalice se smatraju značajnim ekološkim, ekonomskim i socijalnim problemom kako u celom svetu tako i u Republici Srbiji. Psi lutalice zauzimaju posebno mesto u ekologiji velikih gradova i njihov kontakt sa ljudima, predstavlja opasnost, jer oni mogu biti nosioci uzročnika mnogih zoonotskih infektivnih oboljenja. Cilj ovog rada je da se predstavi epizootiološka studija nekih bakterijskih, virusnih i parazitskih oboljenja, pomoću seroloških ispitivanja krvnih seruma i pune krvi pasa. Tokom ovog istraživanja, ispitivana je prevalenca na leptospirozu, tularemiju, groznicu zapadnog Nila i dirofilariozu. Materijal su predstavljali uzorci pune krvi i krvnog seruma pasa lutalica, iz šire okoline grada Leskovca. Metode ispitivanja su obuhvatale standardne serološke testove: test mikroskopske aglutinacije (MAT) za utvrđivanje specifičnih antitela protiv Leptospira spp.; spora i brza aglutinacija za utvrđivanje antitela protiv uzročnika tularaemije (Francisella tularensis); agar gel imunodifuzioni test (AGID) za detekciju specifičnih antitela protiv virusa Zapadnog Nila -WNV; modifikovani Knotov test za detekciju mikrofilarija. Rezultati ispitivanja pokazuju seropozitivnost na uzročnike leptospiroze, tularemije i dirofilarioze, dok nisu dobijeni pozitivni nalazi za WNV. PR Projekat Ministarstva nauke Republike Srbije, br. TR 37015 .
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Seroprevalence of some infectious diseases in stray dogs in the wider territory of Leskovac city
T1  - Seroprevalence nekih zaraznih bolesti u pasa lutalica u širem području Leskovca
VL  - 7
IS  - 1
SP  - 19
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1120
ER  - 

@article{
author = "Manić, Marija and Prokić, Nataša and Gojković, Katarina and Đorić, Goran and Vasić, Ana and Marić, Jovan and Vojinović, Dragica and Đuričić, Bosiljka",
year = "2014",
abstract = "Since the last decade, stray dogs have been significant ecological, economical and sociological problem in the world as well as in the Republic of Serbia. They occupy specifi c place in the ecology of big cities and their contact with human population represents danger since they are carriers of many zoonotic infectious diseases. The goal of this paper is to present epizootiological survey on some diseases of bacterial, viral and parasitic origin through serological investigation of blood sera and full blood. The prevalence of leptospirosis, Tularaemia, West Nile fever and dirofilariosis has been determined in this research. The material included full blood and blood sera of stray dogs in the wider territory of Leskovac city. The research methods encompassed standard serological tests: microscopic agglutination test (MAT) for determination of specific antibodies against Leptospira spp.; slow and fast agglutination test for determination of antibodies against Tularaemia causative agent (Francisella tularensis); agar gel immunodiffusion (AGID) for detection of specific antibodies against West Nile virus -WNV; modified Knot's test for the detection of microfilaria. The results revealed seropositivity for causative agents of Leptospirosis, Tularaemia and dirofilariosis, while there were no positive findings for WNV., Tokom poslednjih deset godina, psi lutalice se smatraju značajnim ekološkim, ekonomskim i socijalnim problemom kako u celom svetu tako i u Republici Srbiji. Psi lutalice zauzimaju posebno mesto u ekologiji velikih gradova i njihov kontakt sa ljudima, predstavlja opasnost, jer oni mogu biti nosioci uzročnika mnogih zoonotskih infektivnih oboljenja. Cilj ovog rada je da se predstavi epizootiološka studija nekih bakterijskih, virusnih i parazitskih oboljenja, pomoću seroloških ispitivanja krvnih seruma i pune krvi pasa. Tokom ovog istraživanja, ispitivana je prevalenca na leptospirozu, tularemiju, groznicu zapadnog Nila i dirofilariozu. Materijal su predstavljali uzorci pune krvi i krvnog seruma pasa lutalica, iz šire okoline grada Leskovca. Metode ispitivanja su obuhvatale standardne serološke testove: test mikroskopske aglutinacije (MAT) za utvrđivanje specifičnih antitela protiv Leptospira spp.; spora i brza aglutinacija za utvrđivanje antitela protiv uzročnika tularaemije (Francisella tularensis); agar gel imunodifuzioni test (AGID) za detekciju specifičnih antitela protiv virusa Zapadnog Nila -WNV; modifikovani Knotov test za detekciju mikrofilarija. Rezultati ispitivanja pokazuju seropozitivnost na uzročnike leptospiroze, tularemije i dirofilarioze, dok nisu dobijeni pozitivni nalazi za WNV. PR Projekat Ministarstva nauke Republike Srbije, br. TR 37015 .",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Seroprevalence of some infectious diseases in stray dogs in the wider territory of Leskovac city, Seroprevalence nekih zaraznih bolesti u pasa lutalica u širem području Leskovca",
volume = "7",
number = "1",
pages = "19-27",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1120"
}

Manić, M., Prokić, N., Gojković, K., Đorić, G., Vasić, A., Marić, J., Vojinović, D.,& Đuričić, B.. (2014). Seroprevalence of some infectious diseases in stray dogs in the wider territory of Leskovac city. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 7(1), 19-27.
https://hdl.handle.net/21.15107/rcub_veterinar_1120

Manić M, Prokić N, Gojković K, Đorić G, Vasić A, Marić J, Vojinović D, Đuričić B. Seroprevalence of some infectious diseases in stray dogs in the wider territory of Leskovac city. in Arhiv veterinarske medicine. 2014;7(1):19-27.
https://hdl.handle.net/21.15107/rcub_veterinar_1120 .

Manić, Marija, Prokić, Nataša, Gojković, Katarina, Đorić, Goran, Vasić, Ana, Marić, Jovan, Vojinović, Dragica, Đuričić, Bosiljka, "Seroprevalence of some infectious diseases in stray dogs in the wider territory of Leskovac city" in Arhiv veterinarske medicine, 7, no. 1 (2014):19-27,
https://hdl.handle.net/21.15107/rcub_veterinar_1120 .

TY  - JOUR
AU  - Đuričić, Bosiljka
AU  - Vasić, Ana
AU  - Rogožarski, Dragan
AU  - Vojinović, Dragica
AU  - Elezović-Radovanović, Milica
AU  - Manić, Marija
AU  - Marić, Jovan
AU  - Prokić, Nataša
AU  - Ilić, Živka
AU  - Novotny, N.
AU  - Gligić, Ana
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/957
AB  - The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping.
AB  - Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia
T1  - Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji
VL  - 63
IS  - 5-6
SP  - 569
EP  - 579
DO  - 10.2298/AVB1306569D
ER  - 

@article{
author = "Đuričić, Bosiljka and Vasić, Ana and Rogožarski, Dragan and Vojinović, Dragica and Elezović-Radovanović, Milica and Manić, Marija and Marić, Jovan and Prokić, Nataša and Ilić, Živka and Novotny, N. and Gligić, Ana",
year = "2013",
abstract = "The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping., Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia, Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji",
volume = "63",
number = "5-6",
pages = "569-579",
doi = "10.2298/AVB1306569D"
}

Đuričić, B., Vasić, A., Rogožarski, D., Vojinović, D., Elezović-Radovanović, M., Manić, M., Marić, J., Prokić, N., Ilić, Ž., Novotny, N.,& Gligić, A.. (2013). Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 569-579.
https://doi.org/10.2298/AVB1306569D

Đuričić B, Vasić A, Rogožarski D, Vojinović D, Elezović-Radovanović M, Manić M, Marić J, Prokić N, Ilić Ž, Novotny N, Gligić A. Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta Veterinaria-Beograd. 2013;63(5-6):569-579.
doi:10.2298/AVB1306569D .

Đuričić, Bosiljka, Vasić, Ana, Rogožarski, Dragan, Vojinović, Dragica, Elezović-Radovanović, Milica, Manić, Marija, Marić, Jovan, Prokić, Nataša, Ilić, Živka, Novotny, N., Gligić, Ana, "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia" in Acta Veterinaria-Beograd, 63, no. 5-6 (2013):569-579,
https://doi.org/10.2298/AVB1306569D . .