TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Mirčeta, Jovan
AU  - Djurdjević, Biljana
AU  - Lazić, Sava
AU  - Aleksić-Kovačević, Sanja
AU  - Petrović, Jelena
AU  - Polaček, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2941
AB  - African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.
PB  - MDPI
T2  - Pathogens
T1  - African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia
VL  - 12
IS  - 5
SP  - 691
DO  - 10.3390/pathogens12050691
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Mirčeta, Jovan and Djurdjević, Biljana and Lazić, Sava and Aleksić-Kovačević, Sanja and Petrović, Jelena and Polaček, Vladimir",
year = "2023",
abstract = "African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.",
publisher = "MDPI",
journal = "Pathogens",
title = "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia",
volume = "12",
number = "5",
pages = "691",
doi = "10.3390/pathogens12050691"
}

Prodanov-Radulović, J., Mirčeta, J., Djurdjević, B., Lazić, S., Aleksić-Kovačević, S., Petrović, J.,& Polaček, V.. (2023). African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens
MDPI., 12(5), 691.
https://doi.org/10.3390/pathogens12050691

Prodanov-Radulović J, Mirčeta J, Djurdjević B, Lazić S, Aleksić-Kovačević S, Petrović J, Polaček V. African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens. 2023;12(5):691.
doi:10.3390/pathogens12050691 .

Prodanov-Radulović, Jasna, Mirčeta, Jovan, Djurdjević, Biljana, Lazić, Sava, Aleksić-Kovačević, Sanja, Petrović, Jelena, Polaček, Vladimir, "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia" in Pathogens, 12, no. 5 (2023):691,
https://doi.org/10.3390/pathogens12050691 . .

TY  - CONF
AU  - Bugarski, Dejan
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Lazić, Sava
AU  - Todorović, Dalibor
AU  - Savić, Sara
AU  - Bojkovski, Jovan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3520
AB  - Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
T1  - Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema
SP  - 51
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3520
ER  - 

@conference{
author = "Bugarski, Dejan and Petrović, Tamaš and Lupulović, Diana and Lazić, Sava and Todorović, Dalibor and Savić, Sara and Bojkovski, Jovan",
year = "2020",
abstract = "Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020",
title = "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema",
pages = "51-62",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3520"
}

Bugarski, D., Petrović, T., Lupulović, D., Lazić, S., Todorović, D., Savić, S.,& Bojkovski, J.. (2020). Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
Beograd : Srpsko veterinarsko društvo., 51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520

Bugarski D, Petrović T, Lupulović D, Lazić S, Todorović D, Savić S, Bojkovski J. Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020. 2020;:51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

Bugarski, Dejan, Petrović, Tamaš, Lupulović, Diana, Lazić, Sava, Todorović, Dalibor, Savić, Sara, Bojkovski, Jovan, "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema" in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020 (2020):51-62,
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 

@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}

Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439

Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .

Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .

TY  - JOUR
AU  - Stanojević, Slavoljub
AU  - Valčić, Miroslav
AU  - Radojičić, Sonja
AU  - Stanojević, Slobodan
AU  - Avramov, Stevan
AU  - Lazić, Sava
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1328
AB  - Several different strategies for control and eradication of Classical Swine Fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analyzed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epizootic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in protection and surveillance zone proved to be the most effective measures to stop spreading and eradication of the disease. However, during the simulation, systematic implementation of biosecurity measures in all pig production clusters demonstrated to be appropriate strategy for sustainable control of CSF and setting up a stable epizootiological situation.
AB  - Nekoliko različitih strategija za kontrolu i iskorenjivanje klasične kuge svinja je upoređivano primenom modela simulacije zasnovanog na Monte Karlo metodi. Kontrolne strategije koje su obrađivane u ovom radu, pored opštih i posebnih mera suzbijanja KKS, uključuju i različite nivoe primenjenih biosigurnosnih mera na komercijalnim, porodičnim farmama i seoskim gazdinstvima na kojima se ga je svinje. Mere neškodljivog uništavanja obolelih životinja i životinja koje su bile u kontaktu sa obolelim životinjama, uspostavljanje zaštitnih zona pod nadzorom, kontrola i zaustavljanje prometa životinja i mehaničkih vektora (vozila i ljudi) unutar zona, rano otkrivanje bolesti i mera vakcinacije su bile uključene u simulaciju. Izlazni parametri kao što su: vreme trajanja epizootije, broj zaraženih gazdinstava i životinja, broj uništenih životinja, troškovi nadzora i direktne štete nastale zbog pojavljivanja bolesti s upoređivani tokom simulacije. Vakcinacija, neškodljivo uklanjanje obolelih životinja i rana dijagnostika su se pokazale kao najefektivnije mere zaustavljanja širenja i iskorenjivanja bolesti. Međutim, kao mera dugoročne strategije kontrole KKS i uspostavljanja stabilne epizootiološke situacije, tokom simulacije pokazala se mera planskog i sistematskog podizanja biosigurnosnih mera u svim klasterima proizvodnje svinja (komercijalne farme, porodične farme tipa A, porodične farme tipa B i seoska gazdinstva).
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Comparative analysis of different strategies for the control of classical swine fever in the Republic of Serbia using Monte Carlo simulation
T1  - Komparativna analiza različitih strategija za kontrolu klasične svinjske kuge upotrebom Monte Carlo simulacije
VL  - 9
IS  - 1
SP  - 43
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1328
ER  - 

@article{
author = "Stanojević, Slavoljub and Valčić, Miroslav and Radojičić, Sonja and Stanojević, Slobodan and Avramov, Stevan and Lazić, Sava and Mišić, Dušan",
year = "2016",
abstract = "Several different strategies for control and eradication of Classical Swine Fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analyzed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epizootic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in protection and surveillance zone proved to be the most effective measures to stop spreading and eradication of the disease. However, during the simulation, systematic implementation of biosecurity measures in all pig production clusters demonstrated to be appropriate strategy for sustainable control of CSF and setting up a stable epizootiological situation., Nekoliko različitih strategija za kontrolu i iskorenjivanje klasične kuge svinja je upoređivano primenom modela simulacije zasnovanog na Monte Karlo metodi. Kontrolne strategije koje su obrađivane u ovom radu, pored opštih i posebnih mera suzbijanja KKS, uključuju i različite nivoe primenjenih biosigurnosnih mera na komercijalnim, porodičnim farmama i seoskim gazdinstvima na kojima se ga je svinje. Mere neškodljivog uništavanja obolelih životinja i životinja koje su bile u kontaktu sa obolelim životinjama, uspostavljanje zaštitnih zona pod nadzorom, kontrola i zaustavljanje prometa životinja i mehaničkih vektora (vozila i ljudi) unutar zona, rano otkrivanje bolesti i mera vakcinacije su bile uključene u simulaciju. Izlazni parametri kao što su: vreme trajanja epizootije, broj zaraženih gazdinstava i životinja, broj uništenih životinja, troškovi nadzora i direktne štete nastale zbog pojavljivanja bolesti s upoređivani tokom simulacije. Vakcinacija, neškodljivo uklanjanje obolelih životinja i rana dijagnostika su se pokazale kao najefektivnije mere zaustavljanja širenja i iskorenjivanja bolesti. Međutim, kao mera dugoročne strategije kontrole KKS i uspostavljanja stabilne epizootiološke situacije, tokom simulacije pokazala se mera planskog i sistematskog podizanja biosigurnosnih mera u svim klasterima proizvodnje svinja (komercijalne farme, porodične farme tipa A, porodične farme tipa B i seoska gazdinstva).",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Comparative analysis of different strategies for the control of classical swine fever in the Republic of Serbia using Monte Carlo simulation, Komparativna analiza različitih strategija za kontrolu klasične svinjske kuge upotrebom Monte Carlo simulacije",
volume = "9",
number = "1",
pages = "43-59",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1328"
}

Stanojević, S., Valčić, M., Radojičić, S., Stanojević, S., Avramov, S., Lazić, S.,& Mišić, D.. (2016). Comparative analysis of different strategies for the control of classical swine fever in the Republic of Serbia using Monte Carlo simulation. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(1), 43-59.
https://hdl.handle.net/21.15107/rcub_veterinar_1328

Stanojević S, Valčić M, Radojičić S, Stanojević S, Avramov S, Lazić S, Mišić D. Comparative analysis of different strategies for the control of classical swine fever in the Republic of Serbia using Monte Carlo simulation. in Arhiv veterinarske medicine. 2016;9(1):43-59.
https://hdl.handle.net/21.15107/rcub_veterinar_1328 .

Stanojević, Slavoljub, Valčić, Miroslav, Radojičić, Sonja, Stanojević, Slobodan, Avramov, Stevan, Lazić, Sava, Mišić, Dušan, "Comparative analysis of different strategies for the control of classical swine fever in the Republic of Serbia using Monte Carlo simulation" in Arhiv veterinarske medicine, 9, no. 1 (2016):43-59,
https://hdl.handle.net/21.15107/rcub_veterinar_1328 .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Borozan, Sunčica
AU  - Zorić, Andrea
AU  - Lazić, Sava
AU  - Petrović, Tamaš
AU  - Rašić, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1221
AB  - The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.
AB  - Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
T1  - Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine
VL  - 69
IS  - 5-6
SP  - 337
EP  - 355
DO  - 10.2298/VETGL1506337M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Borozan, Sunčica and Zorić, Andrea and Lazić, Sava and Petrović, Tamaš and Rašić, Zoran",
year = "2015",
abstract = "The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines., Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus, Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine",
volume = "69",
number = "5-6",
pages = "337-355",
doi = "10.2298/VETGL1506337M"
}

Milić, N., Nišavić, J., Borozan, S., Zorić, A., Lazić, S., Petrović, T.,& Rašić, Z.. (2015). Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(5-6), 337-355.
https://doi.org/10.2298/VETGL1506337M

Milić N, Nišavić J, Borozan S, Zorić A, Lazić S, Petrović T, Rašić Z. Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik. 2015;69(5-6):337-355.
doi:10.2298/VETGL1506337M .

Milić, Nenad, Nišavić, Jakov, Borozan, Sunčica, Zorić, Andrea, Lazić, Sava, Petrović, Tamaš, Rašić, Zoran, "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus" in Veterinarski Glasnik, 69, no. 5-6 (2015):337-355,
https://doi.org/10.2298/VETGL1506337M . .

TY  - JOUR
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Polaček, Vladimir
AU  - Valčić, Miroslav
AU  - Lazić, Gospava
AU  - Pasagić, Enisa
AU  - Petrović, Tamaš
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1180
AB  - The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of equine arteritis virus in the semen of stallions in the republic of Serbia
VL  - 65
IS  - 4
SP  - 557
EP  - 567
DO  - 10.1515/acve-2015-0047
ER  - 

@article{
author = "Lazić, Sava and Lupulović, Diana and Polaček, Vladimir and Valčić, Miroslav and Lazić, Gospava and Pasagić, Enisa and Petrović, Tamaš",
year = "2015",
abstract = "The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia",
volume = "65",
number = "4",
pages = "557-567",
doi = "10.1515/acve-2015-0047"
}

Lazić, S., Lupulović, D., Polaček, V., Valčić, M., Lazić, G., Pasagić, E.,& Petrović, T.. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 557-567.
https://doi.org/10.1515/acve-2015-0047

Lazić S, Lupulović D, Polaček V, Valčić M, Lazić G, Pasagić E, Petrović T. Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):557-567.
doi:10.1515/acve-2015-0047 .

Lazić, Sava, Lupulović, Diana, Polaček, Vladimir, Valčić, Miroslav, Lazić, Gospava, Pasagić, Enisa, Petrović, Tamaš, "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):557-567,
https://doi.org/10.1515/acve-2015-0047 . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Petrić, Dušan
AU  - Vasić, Ana
AU  - Hrnjaković-Cvjetković, Ivana
AU  - Milošević, Vesna
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Lazić, Sava
AU  - Đuričić, Bosiljka
AU  - Plavšić, Budimir
AU  - Juan-Carlos, Saiz
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1205
AB  - West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed.
AB  - Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - WNV infection: An emergent vector borne viral infection in Serbia: Current situation
T1  - Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija
VL  - 69
IS  - 1-2
SP  - 111
EP  - 126
DO  - 10.2298/VETGL1502111P
ER  - 

@article{
author = "Petrović, Tamaš and Lupulović, Diana and Petrić, Dušan and Vasić, Ana and Hrnjaković-Cvjetković, Ivana and Milošević, Vesna and Vidanović, Dejan and Šekler, Milanko and Lazić, Sava and Đuričić, Bosiljka and Plavšić, Budimir and Juan-Carlos, Saiz",
year = "2015",
abstract = "West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed., Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "WNV infection: An emergent vector borne viral infection in Serbia: Current situation, Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija",
volume = "69",
number = "1-2",
pages = "111-126",
doi = "10.2298/VETGL1502111P"
}

Petrović, T., Lupulović, D., Petrić, D., Vasić, A., Hrnjaković-Cvjetković, I., Milošević, V., Vidanović, D., Šekler, M., Lazić, S., Đuričić, B., Plavšić, B.,& Juan-Carlos, S.. (2015). WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 111-126.
https://doi.org/10.2298/VETGL1502111P

Petrović T, Lupulović D, Petrić D, Vasić A, Hrnjaković-Cvjetković I, Milošević V, Vidanović D, Šekler M, Lazić S, Đuričić B, Plavšić B, Juan-Carlos S. WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik. 2015;69(1-2):111-126.
doi:10.2298/VETGL1502111P .

Petrović, Tamaš, Lupulović, Diana, Petrić, Dušan, Vasić, Ana, Hrnjaković-Cvjetković, Ivana, Milošević, Vesna, Vidanović, Dejan, Šekler, Milanko, Lazić, Sava, Đuričić, Bosiljka, Plavšić, Budimir, Juan-Carlos, Saiz, "WNV infection: An emergent vector borne viral infection in Serbia: Current situation" in Veterinarski Glasnik, 69, no. 1-2 (2015):111-126,
https://doi.org/10.2298/VETGL1502111P . .

TY  - JOUR
AU  - Lauš, Saša
AU  - Spasojević-Kosić, Ljubica
AU  - Lazić, Sava
AU  - Trailović, Dragiša
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1210
AB  - The titer of specific antibodies against equine herpesvirus-1 in blood serum was tested in two groups of mares and their foals. The first group consisted of 12 mares, Standardbred and Serbian Trotter breed, who were vaccinated against equine herpesvirus-1 and 4 in the 5th, 7th and 9th month of pregnancy. On the contrary, 12 mares from the second group, of Lipizzaner breed, were not vaccinated. The mares' blood samples for antibodies titer investigation were taken 30, 15 and 7 days before the expected partus, then immediately after the partus, while their foals' blood samples were taken immediately after foaling, then just before colostrum intake, and finally 1, 2, 3 and 7 days later. The titer of antibodies against equine herpesvirus-1 was tested by the method of virus - neutralization, on microtiter plates with constant dose of the virus and serial double dilutions of the serum. In unvaccinated mares, titer of antibodies against equine herpesvirus-1 was either low or not present, but on the contrary, in the vaccinated ones the antibodies titer ranged from 1:32 to 1:256. In the foals originating from both vaccinated and unvaccinated there were not found specific antibodies in the serum before colostrum intake. After the colostrum intake, the values of specific antibodies against equine herpesvirus-1 significantly increased in the foals originating from the vaccinated mares, and ranged from 1:8 to 1:32.
AB  - Titar specifičnih antitela protiv konjskog herpesvirusa-1 u krvnom serumu ispitan je kod dve grupe kobila i njihove ždrebadi. Prva grupa od 12 kobila, rase američki i srpski kasač, bila je vakcinisana protiv konjskog herpsvirusa-1 i 4 u 5, 7. i 9. mesecu graviditeta, za razliku od druge grupe od 12 lipicanskih kobila koje nisu bile vakcinisane. Uzorci krvi kobila za ispitivanje titra antitela bili su uzeti na 30, 15 i 7 dana pre očekivanog partusa, zatim neposredno posle partusa, a uzorci krvi njihove ždrebadi neposredno posle ždrebljenja, pre uzimanja kolostruma, zatim 1, 2, 3 i 7 dana kasnije. Titar antitela protiv konjskog herpesvirusa-1 ispitan je metodom virus - neutralizacije, na mikrotitar pločama sa konstantnom dozom virusa i dvostrukim serijskim razređenjem seruma. Kod nevakcinisanih kobila titar antitela protiv konjskog herpesvirusa-1 bio je nizak ili ga nije bilo, za razliku od vakcinisanih kobila kod kojih je ustanovljen titar atitela u rasponu od 1:32 do 1:256. Kod ždrebadi vakcinisanih i nevakcinisanih kobila nema specifičnih antitela u serumu pre unosa kolostruma. Nakon unosa kolostruma vrednost titra specifičnih antitela na konjski herpesvirus-1 značajno raste kod ždebadi vakcinisanih kobila i kreće se od 1:8 do 1:32.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of presence of antibodies against equine herpesvirus-1 in blood serum of foals prior and after colostral intake
T1  - Ispitivanje prisustva antitela protiv konjskog herpesvirusa-1 u krvnom serumu ždrebadi pre i nakon uzimanja kolostruma
VL  - 69
IS  - 3-4
SP  - 195
EP  - 204
DO  - 10.2298/VETGL1504195L
ER  - 

@article{
author = "Lauš, Saša and Spasojević-Kosić, Ljubica and Lazić, Sava and Trailović, Dragiša",
year = "2015",
abstract = "The titer of specific antibodies against equine herpesvirus-1 in blood serum was tested in two groups of mares and their foals. The first group consisted of 12 mares, Standardbred and Serbian Trotter breed, who were vaccinated against equine herpesvirus-1 and 4 in the 5th, 7th and 9th month of pregnancy. On the contrary, 12 mares from the second group, of Lipizzaner breed, were not vaccinated. The mares' blood samples for antibodies titer investigation were taken 30, 15 and 7 days before the expected partus, then immediately after the partus, while their foals' blood samples were taken immediately after foaling, then just before colostrum intake, and finally 1, 2, 3 and 7 days later. The titer of antibodies against equine herpesvirus-1 was tested by the method of virus - neutralization, on microtiter plates with constant dose of the virus and serial double dilutions of the serum. In unvaccinated mares, titer of antibodies against equine herpesvirus-1 was either low or not present, but on the contrary, in the vaccinated ones the antibodies titer ranged from 1:32 to 1:256. In the foals originating from both vaccinated and unvaccinated there were not found specific antibodies in the serum before colostrum intake. After the colostrum intake, the values of specific antibodies against equine herpesvirus-1 significantly increased in the foals originating from the vaccinated mares, and ranged from 1:8 to 1:32., Titar specifičnih antitela protiv konjskog herpesvirusa-1 u krvnom serumu ispitan je kod dve grupe kobila i njihove ždrebadi. Prva grupa od 12 kobila, rase američki i srpski kasač, bila je vakcinisana protiv konjskog herpsvirusa-1 i 4 u 5, 7. i 9. mesecu graviditeta, za razliku od druge grupe od 12 lipicanskih kobila koje nisu bile vakcinisane. Uzorci krvi kobila za ispitivanje titra antitela bili su uzeti na 30, 15 i 7 dana pre očekivanog partusa, zatim neposredno posle partusa, a uzorci krvi njihove ždrebadi neposredno posle ždrebljenja, pre uzimanja kolostruma, zatim 1, 2, 3 i 7 dana kasnije. Titar antitela protiv konjskog herpesvirusa-1 ispitan je metodom virus - neutralizacije, na mikrotitar pločama sa konstantnom dozom virusa i dvostrukim serijskim razređenjem seruma. Kod nevakcinisanih kobila titar antitela protiv konjskog herpesvirusa-1 bio je nizak ili ga nije bilo, za razliku od vakcinisanih kobila kod kojih je ustanovljen titar atitela u rasponu od 1:32 do 1:256. Kod ždrebadi vakcinisanih i nevakcinisanih kobila nema specifičnih antitela u serumu pre unosa kolostruma. Nakon unosa kolostruma vrednost titra specifičnih antitela na konjski herpesvirus-1 značajno raste kod ždebadi vakcinisanih kobila i kreće se od 1:8 do 1:32.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of presence of antibodies against equine herpesvirus-1 in blood serum of foals prior and after colostral intake, Ispitivanje prisustva antitela protiv konjskog herpesvirusa-1 u krvnom serumu ždrebadi pre i nakon uzimanja kolostruma",
volume = "69",
number = "3-4",
pages = "195-204",
doi = "10.2298/VETGL1504195L"
}

Lauš, S., Spasojević-Kosić, L., Lazić, S.,& Trailović, D.. (2015). Investigations of presence of antibodies against equine herpesvirus-1 in blood serum of foals prior and after colostral intake. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 195-204.
https://doi.org/10.2298/VETGL1504195L

Lauš S, Spasojević-Kosić L, Lazić S, Trailović D. Investigations of presence of antibodies against equine herpesvirus-1 in blood serum of foals prior and after colostral intake. in Veterinarski Glasnik. 2015;69(3-4):195-204.
doi:10.2298/VETGL1504195L .

Lauš, Saša, Spasojević-Kosić, Ljubica, Lazić, Sava, Trailović, Dragiša, "Investigations of presence of antibodies against equine herpesvirus-1 in blood serum of foals prior and after colostral intake" in Veterinarski Glasnik, 69, no. 3-4 (2015):195-204,
https://doi.org/10.2298/VETGL1504195L . .

TY  - JOUR
AU  - Toplak, Ivan
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Prodanov-Radulović, Jasna
AU  - Becskei, Zsolt
AU  - Došen, Radoslav
AU  - Petrović, Tamaš
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/936
AB  - Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia
VL  - 60
IS  - 3
SP  - 409
EP  - 420
DO  - 10.1556/AVet.2012.035
ER  - 

@article{
author = "Toplak, Ivan and Lazić, Sava and Lupulović, Diana and Prodanov-Radulović, Jasna and Becskei, Zsolt and Došen, Radoslav and Petrović, Tamaš",
year = "2012",
abstract = "Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia",
volume = "60",
number = "3",
pages = "409-420",
doi = "10.1556/AVet.2012.035"
}

Toplak, I., Lazić, S., Lupulović, D., Prodanov-Radulović, J., Becskei, Z., Došen, R.,& Petrović, T.. (2012). Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 60(3), 409-420.
https://doi.org/10.1556/AVet.2012.035

Toplak I, Lazić S, Lupulović D, Prodanov-Radulović J, Becskei Z, Došen R, Petrović T. Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica. 2012;60(3):409-420.
doi:10.1556/AVet.2012.035 .

Toplak, Ivan, Lazić, Sava, Lupulović, Diana, Prodanov-Radulović, Jasna, Becskei, Zsolt, Došen, Radoslav, Petrović, Tamaš, "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia" in Acta Veterinaria Hungarica, 60, no. 3 (2012):409-420,
https://doi.org/10.1556/AVet.2012.035 . .

TY  - JOUR
AU  - Lazić, Sava
AU  - Rogan, Dragan
AU  - Petrović, Tamaš
AU  - Bugarski, Dejan
AU  - Lupulović, Diana
AU  - Lazarević, Miodrag
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/723
AB  - The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .
AB  - U radu su prikazani rezultati ispitivanja prisustva antitela protiv goveđeg herpesvirusa-1 (BHV-1) u uzorcima krvnih seruma 106 krava i 107 njihove teladi (jedna krava je otelila blizance). Uzorkovanje krvi od krava je vršeno odmah nakon partusa, a od teladi pre ishrane kolostrumom. Ispitivane krave i njihova telad poticali su iz 5 zapata u kojima je ranijim ispitivanjima utvrđena infekcija goveđim herpesvirusom-1. Utvrđivanje antitela protiv BHV-1 vršeno je metodom virus neutralizacije na kulturi MDBK ćelija sa 100 TCID/50 virusa (BHV-1, TN-41 Am. Bio Research, USA). Antitela protiv BHV-1 utvrđena su u svim uzorcima krvnih seruma krava i u 16 uzoraka prekolostralnih krvnih seruma teladi. Vrednosti titra antitela iznosile su kod krava od 1:4 do 1:512, a kod teladi od 1:2 do 1:16. Rezultati ukazuju na to da krave koje su seropozitivne na BHV-1 mogu da otele telad seropozitivnu na BHV-1 u oko 15% slučajeva. Ovo se mora imati u vidu kada se vrši izbor krava za proizvodnju priplodnog materijala, a posebno bikova za reprocentre, kao i kada se pravi program imunoprofilakse teladi protiv BHV-1. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet
T1  - Ispitivanje prisustva antitela protiv goveđeg herpesvirusa-1 u krvnom serumu teladi pre ishrane kolostrumom
VL  - 64
IS  - 1-2
SP  - 33
EP  - 41
DO  - 10.2298/VETGL1002033L
ER  - 

@article{
author = "Lazić, Sava and Rogan, Dragan and Petrović, Tamaš and Bugarski, Dejan and Lupulović, Diana and Lazarević, Miodrag",
year = "2010",
abstract = "The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. ., U radu su prikazani rezultati ispitivanja prisustva antitela protiv goveđeg herpesvirusa-1 (BHV-1) u uzorcima krvnih seruma 106 krava i 107 njihove teladi (jedna krava je otelila blizance). Uzorkovanje krvi od krava je vršeno odmah nakon partusa, a od teladi pre ishrane kolostrumom. Ispitivane krave i njihova telad poticali su iz 5 zapata u kojima je ranijim ispitivanjima utvrđena infekcija goveđim herpesvirusom-1. Utvrđivanje antitela protiv BHV-1 vršeno je metodom virus neutralizacije na kulturi MDBK ćelija sa 100 TCID/50 virusa (BHV-1, TN-41 Am. Bio Research, USA). Antitela protiv BHV-1 utvrđena su u svim uzorcima krvnih seruma krava i u 16 uzoraka prekolostralnih krvnih seruma teladi. Vrednosti titra antitela iznosile su kod krava od 1:4 do 1:512, a kod teladi od 1:2 do 1:16. Rezultati ukazuju na to da krave koje su seropozitivne na BHV-1 mogu da otele telad seropozitivnu na BHV-1 u oko 15% slučajeva. Ovo se mora imati u vidu kada se vrši izbor krava za proizvodnju priplodnog materijala, a posebno bikova za reprocentre, kao i kada se pravi program imunoprofilakse teladi protiv BHV-1. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet, Ispitivanje prisustva antitela protiv goveđeg herpesvirusa-1 u krvnom serumu teladi pre ishrane kolostrumom",
volume = "64",
number = "1-2",
pages = "33-41",
doi = "10.2298/VETGL1002033L"
}

Lazić, S., Rogan, D., Petrović, T., Bugarski, D., Lupulović, D.,& Lazarević, M.. (2010). Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1-2), 33-41.
https://doi.org/10.2298/VETGL1002033L

Lazić S, Rogan D, Petrović T, Bugarski D, Lupulović D, Lazarević M. Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet. in Veterinarski Glasnik. 2010;64(1-2):33-41.
doi:10.2298/VETGL1002033L .

Lazić, Sava, Rogan, Dragan, Petrović, Tamaš, Bugarski, Dejan, Lupulović, Diana, Lazarević, Miodrag, "Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet" in Veterinarski Glasnik, 64, no. 1-2 (2010):33-41,
https://doi.org/10.2298/VETGL1002033L . .

TY  - JOUR
AU  - Lupulović, Diana
AU  - Milić, Nenad
AU  - Petrović, Tamaš
AU  - Prodanov, Jasna
AU  - Lazić, Sava
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/492
AB  - Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of an endemic infection, all over the world, and also in our country. Timely diagnostics and adequate prophylaxis prevent the occurrence and spread of this disease. Experimental investigations covered 21 sows, divided into two experimental and a third, the control, group. Animals of the first experimental group were vaccinated once before exposure to the boar using an inactivated vaccine, Porcilis Parvo, and animals of the second experimental group were vaccinated twice at an interval of 3 weeks, also using an inactivated vaccine, Parvovax. Sows of the control group were not vaccinated. Blood samples were taken from all animals four times during the course of the experiment, and specific antibodies against the swine parvovirus were determined using the method of hemagglutination inhibition (HI test). The results of the investigations indicate that there was an increase in the titre of specific antibodies following the vaccination of persistently infected sows with the swine parvivirus, and that the present antibodies did not prevent the creating of an immune response. It was established following a comparison of the geometric mean values of antibody titres of vaccinated sows that there was a significant increase in the antibody level following the two vaccinations using the Parvovax vaccine, against the titre values in sows vaccinated once with the Porcilis Parvo vaccine. In control animals, the average value of the antibody level was many times lower in comparison with the established values in the experimental groups. This provides justification for the implementation of immunoprophylaxis against swine parvovirosis by the vaccination of sows and gilts before mating using inactivated vaccines.
AB  - Parvovirusna infekcija svinja je oboljenje koje se manifestuje reproduktivnim poremećajima kod krmača i nazimica u vidu anestrija, prevremenih porođaja, pobačaja, mumifikacijom plodova, rađanjem slabovitalne prasadi i/ili smanjenim brojem prasadi u leglu. Infekcija je na farmama sa intezivnim uzgojem svinja prisutna u vidu endemske infekcije, kako u svetu tako i kod nas. Sprečavanje pojave i širenja ovog obolenja obezbeđuju pravovremena dijagnostika i adekvatna imunoprofilaksa. Eksperimentalnim ispitivanjem obuhvaćena je 21 krmača, koje su svrstane u dve ogledne i treću, kontrolnu grupu. Prva ogledna grupa životinja vakcinisana je pre pripusta jednokratno, inaktivisanom vakcinom Porcilis Parvo, a druga ogledna grupa dvokratno, u intervalu od 3 nedelje, takođe inaktivisanom vakcinom Parvovax. Krmače kontrolne grupe nisu vakcinisane. Uzorkovanje krvi svih životinja vršeno je 4 puta tokom trajanja ogleda, a utvrđivanje specifičnih antitela protiv parvovirusa svinja vršeno je metodom inhibicije hemaglutinacije (HI test). Rezultati ispitivanja ukazuju na porast titra specifičnih antitela posle vakcinacije perzistentno inficiranih krmača parvovirusom svinja, odnosno prisutna antitela nisu sprečila stvaranje imunološkog odgovora. Poređenjem geometrijskih srednjih vrednosti titra antitela vakcinisanih krmača, ustanovljeno je da je značajan rast nivoa antitela nastao posle dvokratne vakcinacije Parvovax vakcinom u odnosu na vrednosti titra kod krmača koje su vakcinisane Porcilis Parvo vakcinom jednokratno. Kod kontrolnih životinja, prosečna vrednost nivoa antitela bila je višestruko niža u poređenju sa utvrđenim vrednostima kod oglednih grupa. Time se smatra opravdanim sprovođenje imunoprofilakse protiv parvoviroze svinja putem vakcinacije krmača i nazimica pre pripusta, inaktivisanim vakcinama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of significance of vaccination against swine parvovirosis in persistently infected sows
T1  - Ispitivanje značaja vakcinacije protiv parvoviroze svinja kod perzistentno inficiranih krmača
VL  - 61
IS  - 5-6
SP  - 251
EP  - 260
DO  - 10.2298/VETGL0706251L
ER  - 

@article{
author = "Lupulović, Diana and Milić, Nenad and Petrović, Tamaš and Prodanov, Jasna and Lazić, Sava",
year = "2007",
abstract = "Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of an endemic infection, all over the world, and also in our country. Timely diagnostics and adequate prophylaxis prevent the occurrence and spread of this disease. Experimental investigations covered 21 sows, divided into two experimental and a third, the control, group. Animals of the first experimental group were vaccinated once before exposure to the boar using an inactivated vaccine, Porcilis Parvo, and animals of the second experimental group were vaccinated twice at an interval of 3 weeks, also using an inactivated vaccine, Parvovax. Sows of the control group were not vaccinated. Blood samples were taken from all animals four times during the course of the experiment, and specific antibodies against the swine parvovirus were determined using the method of hemagglutination inhibition (HI test). The results of the investigations indicate that there was an increase in the titre of specific antibodies following the vaccination of persistently infected sows with the swine parvivirus, and that the present antibodies did not prevent the creating of an immune response. It was established following a comparison of the geometric mean values of antibody titres of vaccinated sows that there was a significant increase in the antibody level following the two vaccinations using the Parvovax vaccine, against the titre values in sows vaccinated once with the Porcilis Parvo vaccine. In control animals, the average value of the antibody level was many times lower in comparison with the established values in the experimental groups. This provides justification for the implementation of immunoprophylaxis against swine parvovirosis by the vaccination of sows and gilts before mating using inactivated vaccines., Parvovirusna infekcija svinja je oboljenje koje se manifestuje reproduktivnim poremećajima kod krmača i nazimica u vidu anestrija, prevremenih porođaja, pobačaja, mumifikacijom plodova, rađanjem slabovitalne prasadi i/ili smanjenim brojem prasadi u leglu. Infekcija je na farmama sa intezivnim uzgojem svinja prisutna u vidu endemske infekcije, kako u svetu tako i kod nas. Sprečavanje pojave i širenja ovog obolenja obezbeđuju pravovremena dijagnostika i adekvatna imunoprofilaksa. Eksperimentalnim ispitivanjem obuhvaćena je 21 krmača, koje su svrstane u dve ogledne i treću, kontrolnu grupu. Prva ogledna grupa životinja vakcinisana je pre pripusta jednokratno, inaktivisanom vakcinom Porcilis Parvo, a druga ogledna grupa dvokratno, u intervalu od 3 nedelje, takođe inaktivisanom vakcinom Parvovax. Krmače kontrolne grupe nisu vakcinisane. Uzorkovanje krvi svih životinja vršeno je 4 puta tokom trajanja ogleda, a utvrđivanje specifičnih antitela protiv parvovirusa svinja vršeno je metodom inhibicije hemaglutinacije (HI test). Rezultati ispitivanja ukazuju na porast titra specifičnih antitela posle vakcinacije perzistentno inficiranih krmača parvovirusom svinja, odnosno prisutna antitela nisu sprečila stvaranje imunološkog odgovora. Poređenjem geometrijskih srednjih vrednosti titra antitela vakcinisanih krmača, ustanovljeno je da je značajan rast nivoa antitela nastao posle dvokratne vakcinacije Parvovax vakcinom u odnosu na vrednosti titra kod krmača koje su vakcinisane Porcilis Parvo vakcinom jednokratno. Kod kontrolnih životinja, prosečna vrednost nivoa antitela bila je višestruko niža u poređenju sa utvrđenim vrednostima kod oglednih grupa. Time se smatra opravdanim sprovođenje imunoprofilakse protiv parvoviroze svinja putem vakcinacije krmača i nazimica pre pripusta, inaktivisanim vakcinama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of significance of vaccination against swine parvovirosis in persistently infected sows, Ispitivanje značaja vakcinacije protiv parvoviroze svinja kod perzistentno inficiranih krmača",
volume = "61",
number = "5-6",
pages = "251-260",
doi = "10.2298/VETGL0706251L"
}

Lupulović, D., Milić, N., Petrović, T., Prodanov, J.,& Lazić, S.. (2007). Investigations of significance of vaccination against swine parvovirosis in persistently infected sows. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 251-260.
https://doi.org/10.2298/VETGL0706251L

Lupulović D, Milić N, Petrović T, Prodanov J, Lazić S. Investigations of significance of vaccination against swine parvovirosis in persistently infected sows. in Veterinarski Glasnik. 2007;61(5-6):251-260.
doi:10.2298/VETGL0706251L .

Lupulović, Diana, Milić, Nenad, Petrović, Tamaš, Prodanov, Jasna, Lazić, Sava, "Investigations of significance of vaccination against swine parvovirosis in persistently infected sows" in Veterinarski Glasnik, 61, no. 5-6 (2007):251-260,
https://doi.org/10.2298/VETGL0706251L . .

TY  - JOUR
AU  - Prodanov, Jasna
AU  - Došen, Radoslav
AU  - Valčić, Miroslav
AU  - Polaček, Vladimir
AU  - Petrović, Tamaš
AU  - Lazić, Sava
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/372
AB  - The pathomorphological changes established following infection with the virus of classical swine plague in non-immune individuals are well known. However, piglets present a problem from the clinical-pathological aspect, in which this virus can be multiplied in spite of established colostral antibodies, but without the exhibiting of the clinical symptoms characteristic for the disease. The question of the characteristics of the pathomorphological finding is raised in the event of the breakdown of the colostral immunity of the piglets. With the objective of determining the influence of colostral antibodies on the development of pathomorphological changes in classical swine plague, piglets aged 28, 35, 44, and 54 days, originating from sows that had received a vaccine of the K-strain of the classical swine plague virus, were experimentally infected with a virulent variety of this virus (Becker strain). The control group comprised non-vaccinated animals originating from non-vaccinated sows. Following the death and/or sacrificing of the piglets in the experiment, a pathomorphological examination was performed of all organic systems and the presence of classical swine plague virus antigens was established in organs and tissues of piglets using the immunoenzyme (ELISA) test. Even though clinical signs characteristic for this disease were not found in all animals following artificial infection, the pathomorphological findings following death and/or sacrificing indicated a successful experimental infection and was typical for the acute course of classical swine plague. Bleeding was established in most organs and serous membranes (haemorrhagic diathesis). However, certain variations were also established regarding the expression and distribution of the pathomorphological changes in certain animals.
AB  - Patomorfološke promene koje se ustanovljavaju posle infekcije izazvane virusom klasične kuge svinja (KKS) kod neimunih jedinki su dobro poznate. Međutim, sa kliničko-patološkog aspekta problem predstavljaju prasad, kod kojih se i pored ustanovljenih kolostralnih antitela virus klasične kuge svinja može da umnožava, ali bez ispoljavanja kliničkih simptoma karakterističnih za KKS. Postavlja se pitanje karakteristika patomorfološkog nalaza u slučaju probijanja kolostralnog imuniteta prasadi. Radi ustanovljavanja uticaja kolostralnih antitela na razvoj patomorfoloških promena kod KKS, izvedena je eksperimentalna infekcija virulentnim virusom KKS (soj Beker) prasadi u uzrastu 28, 35, 44. i 54. dana koja potiču od krmača vakcinisanih K-sojem virusa KKS. Kontrolna grupa je obuhvatala nevakcinisane jedinke, koje potiču od nevakcinisanih krmača. Posle uginuća i/ili žrtvovanja prasadi u ogledu, obavljen je patomorfološki pregled svih organskih sistema i prisustvo antigena virusa KKS je utvrđivano u organima i tkivima prasadi imunoenzimskim (ELISA) testom. Iako nakon veštačke infekcije kod svih jedinki nisu ustanovljeni klinički znaci karakteristični za klasičnu kugu svinja, patomorfološki nalaz posle uginuća i/ili žrtvovanja je ukazao na uspešnu eksperimentalnu infekciju i bio je tipičan za akutni tok KKS. Ustanovljena su krvarenja u većini organa i seroznih membrana (he- moragična dijateza). Međutim, utvrđene su i izvesne varijacije u pogledu izraženosti i distribucije patomorfoloških promena kod pojednih jedinki.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of influence of colostral antibodies on development of pathomorphological changes following experimental infection of piglets with classical swine plague virus
T1  - Ispitivanje uticaja kolostralnih antitela na razvoj patomorfoloških promena posle eksperimentalne infekcije prasadi virusom klasične kuge svinja
VL  - 60
IS  - 5-6
SP  - 323
EP  - 335
DO  - 10.2298/VETGL0606323P
ER  - 

@article{
author = "Prodanov, Jasna and Došen, Radoslav and Valčić, Miroslav and Polaček, Vladimir and Petrović, Tamaš and Lazić, Sava",
year = "2006",
abstract = "The pathomorphological changes established following infection with the virus of classical swine plague in non-immune individuals are well known. However, piglets present a problem from the clinical-pathological aspect, in which this virus can be multiplied in spite of established colostral antibodies, but without the exhibiting of the clinical symptoms characteristic for the disease. The question of the characteristics of the pathomorphological finding is raised in the event of the breakdown of the colostral immunity of the piglets. With the objective of determining the influence of colostral antibodies on the development of pathomorphological changes in classical swine plague, piglets aged 28, 35, 44, and 54 days, originating from sows that had received a vaccine of the K-strain of the classical swine plague virus, were experimentally infected with a virulent variety of this virus (Becker strain). The control group comprised non-vaccinated animals originating from non-vaccinated sows. Following the death and/or sacrificing of the piglets in the experiment, a pathomorphological examination was performed of all organic systems and the presence of classical swine plague virus antigens was established in organs and tissues of piglets using the immunoenzyme (ELISA) test. Even though clinical signs characteristic for this disease were not found in all animals following artificial infection, the pathomorphological findings following death and/or sacrificing indicated a successful experimental infection and was typical for the acute course of classical swine plague. Bleeding was established in most organs and serous membranes (haemorrhagic diathesis). However, certain variations were also established regarding the expression and distribution of the pathomorphological changes in certain animals., Patomorfološke promene koje se ustanovljavaju posle infekcije izazvane virusom klasične kuge svinja (KKS) kod neimunih jedinki su dobro poznate. Međutim, sa kliničko-patološkog aspekta problem predstavljaju prasad, kod kojih se i pored ustanovljenih kolostralnih antitela virus klasične kuge svinja može da umnožava, ali bez ispoljavanja kliničkih simptoma karakterističnih za KKS. Postavlja se pitanje karakteristika patomorfološkog nalaza u slučaju probijanja kolostralnog imuniteta prasadi. Radi ustanovljavanja uticaja kolostralnih antitela na razvoj patomorfoloških promena kod KKS, izvedena je eksperimentalna infekcija virulentnim virusom KKS (soj Beker) prasadi u uzrastu 28, 35, 44. i 54. dana koja potiču od krmača vakcinisanih K-sojem virusa KKS. Kontrolna grupa je obuhvatala nevakcinisane jedinke, koje potiču od nevakcinisanih krmača. Posle uginuća i/ili žrtvovanja prasadi u ogledu, obavljen je patomorfološki pregled svih organskih sistema i prisustvo antigena virusa KKS je utvrđivano u organima i tkivima prasadi imunoenzimskim (ELISA) testom. Iako nakon veštačke infekcije kod svih jedinki nisu ustanovljeni klinički znaci karakteristični za klasičnu kugu svinja, patomorfološki nalaz posle uginuća i/ili žrtvovanja je ukazao na uspešnu eksperimentalnu infekciju i bio je tipičan za akutni tok KKS. Ustanovljena su krvarenja u većini organa i seroznih membrana (he- moragična dijateza). Međutim, utvrđene su i izvesne varijacije u pogledu izraženosti i distribucije patomorfoloških promena kod pojednih jedinki.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of influence of colostral antibodies on development of pathomorphological changes following experimental infection of piglets with classical swine plague virus, Ispitivanje uticaja kolostralnih antitela na razvoj patomorfoloških promena posle eksperimentalne infekcije prasadi virusom klasične kuge svinja",
volume = "60",
number = "5-6",
pages = "323-335",
doi = "10.2298/VETGL0606323P"
}

Prodanov, J., Došen, R., Valčić, M., Polaček, V., Petrović, T.,& Lazić, S.. (2006). Investigations of influence of colostral antibodies on development of pathomorphological changes following experimental infection of piglets with classical swine plague virus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 323-335.
https://doi.org/10.2298/VETGL0606323P

Prodanov J, Došen R, Valčić M, Polaček V, Petrović T, Lazić S. Investigations of influence of colostral antibodies on development of pathomorphological changes following experimental infection of piglets with classical swine plague virus. in Veterinarski Glasnik. 2006;60(5-6):323-335.
doi:10.2298/VETGL0606323P .

Prodanov, Jasna, Došen, Radoslav, Valčić, Miroslav, Polaček, Vladimir, Petrović, Tamaš, Lazić, Sava, "Investigations of influence of colostral antibodies on development of pathomorphological changes following experimental infection of piglets with classical swine plague virus" in Veterinarski Glasnik, 60, no. 5-6 (2006):323-335,
https://doi.org/10.2298/VETGL0606323P . .