TY  - JOUR
AU  - Ilić Božović, Anja
AU  - Đoković, Petar
AU  - Milanović, Zorana
AU  - Janjić, Filip
AU  - Spariosu, Kristina
AU  - Radonjić, Vladimir
AU  - Radaković, Milena
AU  - Magaš, Vladimir
AU  - Filipović, Dimitrije
AU  - Stanković, Sanja
AU  - Kovačević Filipović, Milica
AU  - Beletić, Andelo
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2934
AB  - Homocysteine (Hcy) was investigated as the biomarker of cardiac, renal, and gastrointestinal disorders
in dogs. Data about low Hcy concentrations in the systemic inflammatory response syndrome raised a hypothesis that
Hcy in dogs could be a negative acute-phase reactant. This survey compared Hcy concentrations, serum amyloid A
(SAA), and the routine laboratory parameters between healthy (HD, N=6) and dogs with inflammation of different
extent: mild (dirofilariosis (DIR), N=31), moderate (babesiosis (BAB), N=12), and severe (pyometra (PYO), N=8).
The BAB and PYO groups had lower Hcy er than HD. Also, the levels in the PYO group were below those in the
DIRO group. SAA had the inverse pattern. Across the groups, Hcy and SAA levels correlated negatively (ρ = -0.502,
P<0.001). Hcy and SAA correlated with the erythrocyte count, hematocrit, hemoglobin and mean cellular hemoglobin
concentrations, and neutrophil count, with correlations being positive for Hcy and negative for SAA. Among all dogs,
hemoglobin was the only independent predictor of Hcy concentration. Hcy levels in canine infections, decreased as
acute-phase reaction (APR) intensified. Also, they were related with the hematology changes accompanying the APR.
Further studies will establish the clinical potential of these alterations.
PB  - Hellenic Veterinary Medical Society
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?
VL  - 74
IS  - 1
SP  - 5463
EP  - 5470
DO  - 10.12681/jhvms.29529
ER  - 

@article{
author = "Ilić Božović, Anja and Đoković, Petar and Milanović, Zorana and Janjić, Filip and Spariosu, Kristina and Radonjić, Vladimir and Radaković, Milena and Magaš, Vladimir and Filipović, Dimitrije and Stanković, Sanja and Kovačević Filipović, Milica and Beletić, Andelo",
year = "2023",
abstract = "Homocysteine (Hcy) was investigated as the biomarker of cardiac, renal, and gastrointestinal disorders
in dogs. Data about low Hcy concentrations in the systemic inflammatory response syndrome raised a hypothesis that
Hcy in dogs could be a negative acute-phase reactant. This survey compared Hcy concentrations, serum amyloid A
(SAA), and the routine laboratory parameters between healthy (HD, N=6) and dogs with inflammation of different
extent: mild (dirofilariosis (DIR), N=31), moderate (babesiosis (BAB), N=12), and severe (pyometra (PYO), N=8).
The BAB and PYO groups had lower Hcy er than HD. Also, the levels in the PYO group were below those in the
DIRO group. SAA had the inverse pattern. Across the groups, Hcy and SAA levels correlated negatively (ρ = -0.502,
P<0.001). Hcy and SAA correlated with the erythrocyte count, hematocrit, hemoglobin and mean cellular hemoglobin
concentrations, and neutrophil count, with correlations being positive for Hcy and negative for SAA. Among all dogs,
hemoglobin was the only independent predictor of Hcy concentration. Hcy levels in canine infections, decreased as
acute-phase reaction (APR) intensified. Also, they were related with the hematology changes accompanying the APR.
Further studies will establish the clinical potential of these alterations.",
publisher = "Hellenic Veterinary Medical Society",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?",
volume = "74",
number = "1",
pages = "5463-5470",
doi = "10.12681/jhvms.29529"
}

Ilić Božović, A., Đoković, P., Milanović, Z., Janjić, F., Spariosu, K., Radonjić, V., Radaković, M., Magaš, V., Filipović, D., Stanković, S., Kovačević Filipović, M.,& Beletić, A.. (2023). Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Society., 74(1), 5463-5470.
https://doi.org/10.12681/jhvms.29529

Ilić Božović A, Đoković P, Milanović Z, Janjić F, Spariosu K, Radonjić V, Radaković M, Magaš V, Filipović D, Stanković S, Kovačević Filipović M, Beletić A. Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?. in Journal of the Hellenic Veterinary Medical Society. 2023;74(1):5463-5470.
doi:10.12681/jhvms.29529 .

Ilić Božović, Anja, Đoković, Petar, Milanović, Zorana, Janjić, Filip, Spariosu, Kristina, Radonjić, Vladimir, Radaković, Milena, Magaš, Vladimir, Filipović, Dimitrije, Stanković, Sanja, Kovačević Filipović, Milica, Beletić, Andelo, "Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?" in Journal of the Hellenic Veterinary Medical Society, 74, no. 1 (2023):5463-5470,
https://doi.org/10.12681/jhvms.29529 . .

TY  - CONF
AU  - Ilić Božović, Anja
AU  - Milanović, Zorana
AU  - Janjić, Filip
AU  - Spariosu, Kristina
AU  - Radonjić, Vladimir
AU  - Filipović, Dimitrije
AU  - Stanković, Sanja
AU  - Kovačević Filipović, Milica
AU  - Beletić, Anđelo
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2616
AB  - Background: Lower homocysteine (Hcy) concentraon was noced in dogs with
systemic inflammatory response syndrome of various aeology, in comparison with
healthy dogs. Objecve. The study had two objecves: to compare Hcy
concentraons in serum between dogs naturally infected with Babesia canis and
healthy dogs; to assess the relaonship between Hcy concentraon and the results
of the roune laboratory tests. Materials and methods. The study enrolled twelve
dogs infected with B. canis (BAB) and six healthy dogs (HD). The commercial
immunoassay, spectrophotometry, and haematology kits were used. Stascal
analyses included the Mann-Whitney U test and Spearman's rank correlaon
analysis. Results: BAB had lower concentraons of Hcy than HD (p=0.045). When
analysing the results from all studied dogs together, Hcy had a posive correlaon
with erythrocyte count (r=0.771; p=0.001), haemoglobin concentraon (r=0.696;
p=0.004), haematocrit (r=0.664; p=0.007), leukocyte count (r=0.554; p=0.032), and
total protein concentraon (r=0.689; p=0.004), and a negave with glycaemia (r=-
0.693; p=0.004). Conclusion: The obtained results provide preliminary evidence
about the associaon between acute canine babesiosis and lowered Hcy
concentraon in blood. Further studies will decipher whether this finding has
clinical applicability or represents just an APR epiphenomenon.
C3  - 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022
T1  - Homocysteine concentration in serum of dogs naturally infected with Babesia canis
SP  - 82
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2616
ER  - 

@conference{
author = "Ilić Božović, Anja and Milanović, Zorana and Janjić, Filip and Spariosu, Kristina and Radonjić, Vladimir and Filipović, Dimitrije and Stanković, Sanja and Kovačević Filipović, Milica and Beletić, Anđelo",
year = "2022",
abstract = "Background: Lower homocysteine (Hcy) concentraon was noced in dogs with
systemic inflammatory response syndrome of various aeology, in comparison with
healthy dogs. Objecve. The study had two objecves: to compare Hcy
concentraons in serum between dogs naturally infected with Babesia canis and
healthy dogs; to assess the relaonship between Hcy concentraon and the results
of the roune laboratory tests. Materials and methods. The study enrolled twelve
dogs infected with B. canis (BAB) and six healthy dogs (HD). The commercial
immunoassay, spectrophotometry, and haematology kits were used. Stascal
analyses included the Mann-Whitney U test and Spearman's rank correlaon
analysis. Results: BAB had lower concentraons of Hcy than HD (p=0.045). When
analysing the results from all studied dogs together, Hcy had a posive correlaon
with erythrocyte count (r=0.771; p=0.001), haemoglobin concentraon (r=0.696;
p=0.004), haematocrit (r=0.664; p=0.007), leukocyte count (r=0.554; p=0.032), and
total protein concentraon (r=0.689; p=0.004), and a negave with glycaemia (r=-
0.693; p=0.004). Conclusion: The obtained results provide preliminary evidence
about the associaon between acute canine babesiosis and lowered Hcy
concentraon in blood. Further studies will decipher whether this finding has
clinical applicability or represents just an APR epiphenomenon.",
journal = "24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022",
title = "Homocysteine concentration in serum of dogs naturally infected with Babesia canis",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2616"
}

Ilić Božović, A., Milanović, Z., Janjić, F., Spariosu, K., Radonjić, V., Filipović, D., Stanković, S., Kovačević Filipović, M.,& Beletić, A.. (2022). Homocysteine concentration in serum of dogs naturally infected with Babesia canis. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022, 82-82.
https://hdl.handle.net/21.15107/rcub_veterinar_2616

Ilić Božović A, Milanović Z, Janjić F, Spariosu K, Radonjić V, Filipović D, Stanković S, Kovačević Filipović M, Beletić A. Homocysteine concentration in serum of dogs naturally infected with Babesia canis. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022. 2022;:82-82.
https://hdl.handle.net/21.15107/rcub_veterinar_2616 .

Ilić Božović, Anja, Milanović, Zorana, Janjić, Filip, Spariosu, Kristina, Radonjić, Vladimir, Filipović, Dimitrije, Stanković, Sanja, Kovačević Filipović, Milica, Beletić, Anđelo, "Homocysteine concentration in serum of dogs naturally infected with Babesia canis" in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022 (2022):82-82,
https://hdl.handle.net/21.15107/rcub_veterinar_2616 .

TY  - JOUR
AU  - Antić, Milutin
AU  - Beletić, Anđelo
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Stanković, Sanja
AU  - Trailović, Dragiša
AU  - Kovačević Filipović, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2718
AB  - Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of
immune complexes, which induces vasculitis and leads to endothelial dysfunction.
Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning
its concentration in different CL stages and links to the acute phase response and
oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24)
and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs
and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy
administration, had more distinct clinical signs, lower erythron values, and a higher level
of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than
dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the
lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage,
therapy, levels of IgG, and clinical pathology data. The only positive relationship existed
between Hcy and the erythron state. The disease stage and therapeutic intervention
were not related to the oxidative stress level, except in the case of paraoxonase-1/
Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In
conclusion, changes in Hcy levels indicated its possible involvement with endothelial
dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy
might be an early marker of favorable conditions for endothelium recovery in CL
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria
T1  - Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data
VL  - 72
IS  - 3
SP  - 309
EP  - 323
DO  - 10.2478/acve-2022-0025
ER  - 

@article{
author = "Antić, Milutin and Beletić, Anđelo and Radaković, Milena and Spariosu, Kristina and Stanković, Sanja and Trailović, Dragiša and Kovačević Filipović, Milica",
year = "2022",
abstract = "Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of
immune complexes, which induces vasculitis and leads to endothelial dysfunction.
Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning
its concentration in different CL stages and links to the acute phase response and
oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24)
and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs
and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy
administration, had more distinct clinical signs, lower erythron values, and a higher level
of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than
dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the
lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage,
therapy, levels of IgG, and clinical pathology data. The only positive relationship existed
between Hcy and the erythron state. The disease stage and therapeutic intervention
were not related to the oxidative stress level, except in the case of paraoxonase-1/
Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In
conclusion, changes in Hcy levels indicated its possible involvement with endothelial
dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy
might be an early marker of favorable conditions for endothelium recovery in CL",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria",
title = "Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data",
volume = "72",
number = "3",
pages = "309-323",
doi = "10.2478/acve-2022-0025"
}

Antić, M., Beletić, A., Radaković, M., Spariosu, K., Stanković, S., Trailović, D.,& Kovačević Filipović, M.. (2022). Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data. in Acta Veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(3), 309-323.
https://doi.org/10.2478/acve-2022-0025

Antić M, Beletić A, Radaković M, Spariosu K, Stanković S, Trailović D, Kovačević Filipović M. Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data. in Acta Veterinaria. 2022;72(3):309-323.
doi:10.2478/acve-2022-0025 .

Antić, Milutin, Beletić, Anđelo, Radaković, Milena, Spariosu, Kristina, Stanković, Sanja, Trailović, Dragiša, Kovačević Filipović, Milica, "Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data" in Acta Veterinaria, 72, no. 3 (2022):309-323,
https://doi.org/10.2478/acve-2022-0025 . .

TY  - JOUR
AU  - Čobanović, Nikola
AU  - Stajković, Silvana
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Stanković, Sanja Dj
AU  - Karabasil, Nedjeljko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1993
AB  - This study examined the relationship between lung lesion severity and presence of antibodies of various respiratory pathogens, and the effects of lung lesion severity on growth performance, biochemical indicators, total aerobe counts, and carcass and meat quality indicators in total of 240 slaughter pigs originating from two farms with similar rearing conditions. Lung lesion severity was calculated based on the degree of pneumonia and pleurisy in slaughtered pigs. Two-step cluster analysis was used to place individual pigs to four clusters according to pneumonia and pleurisy scores: no lung lesions (cluster 1); mild lung lesions (cluster 2); moderate lung lesions (cluster 3); and severe lung lesions (cluster 4). ANOVA and post hoc pairwise comparisons using Tukey's test were performed to assess the differences between clusters in examined variables. Multivariate linear regression analysis was run to identify associations between lung lesions and examined variables. There was a strong evidence of association between the absence of lung lesions and increased albumin, sodium and chloride levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and decreased haptoglobin, CK and LDH levels. Also, pigs without lung lesions produced the highest percentage of red, firm and nonexudative pork. Pigs having severe lung lesions had the highest percentage of simultaneously seropositive samples to SIV, PRRSV, PCV-2, PRCV, APP and M. hyopneumoniae. There was a strong evidence of association between the presence of severe lung lesions and decreased lactate, glucose, sodium, chloride and albumine levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and increased CK, LDH and haptoglobin levels. There was a strong evidence of association between the presence of severe lung lesions in slaughered pigs and increased meat pH and sensory colour scores, and decreased drip, thawing and cooking losses and L* and b* values, which led to the highest occurrence of moderate DFD and DFD pork. There was a strong evidence of association between the presence of mild lung lesions in slaughtered pigs and decreased meat pH and sensory colour scores, and increased drip, thawing and cooking losses, L* and b* values, which led to the highest occurrence of moderate PSE and PSE pork. In conclusion, the presence of lung lesions, irrespective of severity, was significantly associated with alterations in the biochemical indicators, growth performance and carcass and meat quality in slaughtered pigs.
PB  - Elsevier
T2  - Preventive Veterinary Medicine
T1  - Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs
VL  - 188
DO  - 10.1016/j.prevetmed.2021.105269
ER  - 

@article{
author = "Čobanović, Nikola and Stajković, Silvana and Kureljušić, Jasna and Žutić, Jadranka and Kureljušić, Branislav and Stanković, Sanja Dj and Karabasil, Nedjeljko",
year = "2021",
abstract = "This study examined the relationship between lung lesion severity and presence of antibodies of various respiratory pathogens, and the effects of lung lesion severity on growth performance, biochemical indicators, total aerobe counts, and carcass and meat quality indicators in total of 240 slaughter pigs originating from two farms with similar rearing conditions. Lung lesion severity was calculated based on the degree of pneumonia and pleurisy in slaughtered pigs. Two-step cluster analysis was used to place individual pigs to four clusters according to pneumonia and pleurisy scores: no lung lesions (cluster 1); mild lung lesions (cluster 2); moderate lung lesions (cluster 3); and severe lung lesions (cluster 4). ANOVA and post hoc pairwise comparisons using Tukey's test were performed to assess the differences between clusters in examined variables. Multivariate linear regression analysis was run to identify associations between lung lesions and examined variables. There was a strong evidence of association between the absence of lung lesions and increased albumin, sodium and chloride levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and decreased haptoglobin, CK and LDH levels. Also, pigs without lung lesions produced the highest percentage of red, firm and nonexudative pork. Pigs having severe lung lesions had the highest percentage of simultaneously seropositive samples to SIV, PRRSV, PCV-2, PRCV, APP and M. hyopneumoniae. There was a strong evidence of association between the presence of severe lung lesions and decreased lactate, glucose, sodium, chloride and albumine levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and increased CK, LDH and haptoglobin levels. There was a strong evidence of association between the presence of severe lung lesions in slaughered pigs and increased meat pH and sensory colour scores, and decreased drip, thawing and cooking losses and L* and b* values, which led to the highest occurrence of moderate DFD and DFD pork. There was a strong evidence of association between the presence of mild lung lesions in slaughtered pigs and decreased meat pH and sensory colour scores, and increased drip, thawing and cooking losses, L* and b* values, which led to the highest occurrence of moderate PSE and PSE pork. In conclusion, the presence of lung lesions, irrespective of severity, was significantly associated with alterations in the biochemical indicators, growth performance and carcass and meat quality in slaughtered pigs.",
publisher = "Elsevier",
journal = "Preventive Veterinary Medicine",
title = "Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs",
volume = "188",
doi = "10.1016/j.prevetmed.2021.105269"
}

Čobanović, N., Stajković, S., Kureljušić, J., Žutić, J., Kureljušić, B., Stanković, S. D.,& Karabasil, N.. (2021). Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs. in Preventive Veterinary Medicine
Elsevier., 188.
https://doi.org/10.1016/j.prevetmed.2021.105269

Čobanović N, Stajković S, Kureljušić J, Žutić J, Kureljušić B, Stanković SD, Karabasil N. Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs. in Preventive Veterinary Medicine. 2021;188.
doi:10.1016/j.prevetmed.2021.105269 .

Čobanović, Nikola, Stajković, Silvana, Kureljušić, Jasna, Žutić, Jadranka, Kureljušić, Branislav, Stanković, Sanja Dj, Karabasil, Nedjeljko, "Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs" in Preventive Veterinary Medicine, 188 (2021),
https://doi.org/10.1016/j.prevetmed.2021.105269 . .