TY  - JOUR
AU  - Tešović, Bojana
AU  - Nišavić, Jakov
AU  - Banović-Đeri, Bojana
AU  - Petrović, Tamaš
AU  - Radalj, Andrea
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Debeljak, Zoran
AU  - Vasković, Nikola
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2520
AB  - West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.
PB  - Elsevier
T2  - Diagnostic Microbiology and Infectious Disease
T1  - Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform
VL  - 105
IS  - 2
SP  - 115852
DO  - 10.1016/j.diagmicrobio.2022.115852
ER  - 

@article{
author = "Tešović, Bojana and Nišavić, Jakov and Banović-Đeri, Bojana and Petrović, Tamaš and Radalj, Andrea and Šekler, Milanko and Matović, Kazimir and Debeljak, Zoran and Vasković, Nikola and Dmitrić, Marko and Vidanović, Dejan",
year = "2023",
abstract = "West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.",
publisher = "Elsevier",
journal = "Diagnostic Microbiology and Infectious Disease",
title = "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform",
volume = "105",
number = "2",
pages = "115852",
doi = "10.1016/j.diagmicrobio.2022.115852"
}

Tešović, B., Nišavić, J., Banović-Đeri, B., Petrović, T., Radalj, A., Šekler, M., Matović, K., Debeljak, Z., Vasković, N., Dmitrić, M.,& Vidanović, D.. (2023). Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease
Elsevier., 105(2), 115852.
https://doi.org/10.1016/j.diagmicrobio.2022.115852

Tešović B, Nišavić J, Banović-Đeri B, Petrović T, Radalj A, Šekler M, Matović K, Debeljak Z, Vasković N, Dmitrić M, Vidanović D. Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease. 2023;105(2):115852.
doi:10.1016/j.diagmicrobio.2022.115852 .

Tešović, Bojana, Nišavić, Jakov, Banović-Đeri, Bojana, Petrović, Tamaš, Radalj, Andrea, Šekler, Milanko, Matović, Kazimir, Debeljak, Zoran, Vasković, Nikola, Dmitrić, Marko, Vidanović, Dejan, "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform" in Diagnostic Microbiology and Infectious Disease, 105, no. 2 (2023):115852,
https://doi.org/10.1016/j.diagmicrobio.2022.115852 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Debeljak, Mihailo
AU  - Stojanović, Milovan
AU  - Glavčić, Marko
AU  - Marinković, Darko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3340
AB  - Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp.
AB  - Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Prikaz slučaja leptospiroze kod dve alpake
T1  - Leptospirosis in two alpacas - case report
SP  - 134
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3340
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Debeljak, Mihailo and Stojanović, Milovan and Glavčić, Marko and Marinković, Darko",
year = "2023",
abstract = "Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp., Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Prikaz slučaja leptospiroze kod dve alpake, Leptospirosis in two alpacas - case report",
pages = "134-135",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3340"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Debeljak, M., Stojanović, M., Glavčić, M.,& Marinković, D.. (2023). Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Debeljak M, Stojanović M, Glavčić M, Marinković D. Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Debeljak, Mihailo, Stojanović, Milovan, Glavčić, Marko, Marinković, Darko, "Prikaz slučaja leptospiroze kod dve alpake" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):134-135,
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

TY  - JOUR
AU  - Nešić, Vladimir
AU  - Marinković, Darko
AU  - Matović, Kazimir
AU  - Radaković, Miloš
AU  - Davitkov, Darko
AU  - Vasković, Nikola
AU  - Davitkov, Dajana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2476
AB  - Although Mycobacterium spp. often cause disease in domestic birds (chickens and companion birds), there are few data on avian tuberculosis in wild populations, especially in birds of prey. We describe here a case of a young adult female, free-living Eurasian griffon vulture (Gyps fulvus) that was found dead. Granulomas were grossly evident in the lungs at autopsy, and tuberculosis was suspected. Ziehl–Neelsen staining revealed large numbers of intracellular acid-fast–positive bacteria within granulomas. Examination on Löwenstein–Jensen medium was negative, but mycobacteria growth indicator tube medium results were positive. For the molecular detection of Mycobacterium spp., the primer set IS901F and IS901R was used. Positive results were observed on gel electrophoresis, indicating the presence of Mycobacterium avium subsp. avium DNA. Although tuberculosis is not considered to be a common cause of death in wild birds, it undoubtedly deserves special attention because vultures are generally considered to be a species resistant to a large number of pathogens. Determination of the cause of death of griffon vultures is important for future conservation measures for this sensitive wild species.
PB  - SAGE
T2  - Journal of Veterinary Diagnostic Investigation
T1  - Avian tuberculosis in a free-living Eurasian griffon vulture
VL  - 34
IS  - 4
SP  - 723
EP  - 726
DO  - 10.1177/10406387221102432
ER  - 

@article{
author = "Nešić, Vladimir and Marinković, Darko and Matović, Kazimir and Radaković, Miloš and Davitkov, Darko and Vasković, Nikola and Davitkov, Dajana",
year = "2022",
abstract = "Although Mycobacterium spp. often cause disease in domestic birds (chickens and companion birds), there are few data on avian tuberculosis in wild populations, especially in birds of prey. We describe here a case of a young adult female, free-living Eurasian griffon vulture (Gyps fulvus) that was found dead. Granulomas were grossly evident in the lungs at autopsy, and tuberculosis was suspected. Ziehl–Neelsen staining revealed large numbers of intracellular acid-fast–positive bacteria within granulomas. Examination on Löwenstein–Jensen medium was negative, but mycobacteria growth indicator tube medium results were positive. For the molecular detection of Mycobacterium spp., the primer set IS901F and IS901R was used. Positive results were observed on gel electrophoresis, indicating the presence of Mycobacterium avium subsp. avium DNA. Although tuberculosis is not considered to be a common cause of death in wild birds, it undoubtedly deserves special attention because vultures are generally considered to be a species resistant to a large number of pathogens. Determination of the cause of death of griffon vultures is important for future conservation measures for this sensitive wild species.",
publisher = "SAGE",
journal = "Journal of Veterinary Diagnostic Investigation",
title = "Avian tuberculosis in a free-living Eurasian griffon vulture",
volume = "34",
number = "4",
pages = "723-726",
doi = "10.1177/10406387221102432"
}

Nešić, V., Marinković, D., Matović, K., Radaković, M., Davitkov, D., Vasković, N.,& Davitkov, D.. (2022). Avian tuberculosis in a free-living Eurasian griffon vulture. in Journal of Veterinary Diagnostic Investigation
SAGE., 34(4), 723-726.
https://doi.org/10.1177/10406387221102432

Nešić V, Marinković D, Matović K, Radaković M, Davitkov D, Vasković N, Davitkov D. Avian tuberculosis in a free-living Eurasian griffon vulture. in Journal of Veterinary Diagnostic Investigation. 2022;34(4):723-726.
doi:10.1177/10406387221102432 .

Nešić, Vladimir, Marinković, Darko, Matović, Kazimir, Radaković, Miloš, Davitkov, Darko, Vasković, Nikola, Davitkov, Dajana, "Avian tuberculosis in a free-living Eurasian griffon vulture" in Journal of Veterinary Diagnostic Investigation, 34, no. 4 (2022):723-726,
https://doi.org/10.1177/10406387221102432 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3175
AB  - Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести.
AB  - Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
T1  - Prikaz slučaja Aujeskijeve bolesti kod lovnog psa
T1  - Aujezsky’s disease in a hunting dog - case report
SP  - 52
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3175
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2022",
abstract = "Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести., Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022",
title = "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa, Aujezsky’s disease in a hunting dog - case report",
pages = "52-53",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3175"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2022). Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022. 2022;:52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa" in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022 (2022):52-53,
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2926
AB  - Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса.
AB  - Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
T1  - Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji
T1  - Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2926
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Aničić, Milan and Marinković, Darko",
year = "2021",
abstract = "Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса., Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021",
title = "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji, Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2926"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Aničić, M.,& Marinković, D.. (2021). Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Aničić M, Marinković D. Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021. 2021;:140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Aničić, Milan, Marinković, Darko, "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji" in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021 (2021):140-141,
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Radojičić, Sonja
AU  - Debeljak, Zoran
AU  - Šekler, Milanko
AU  - Ćirić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797
AB  - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
PB  - Elsevier, Amsterdam
T2  - Saudi Journal of Biological Sciences
T1  - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
VL  - 27
IS  - 1
SP  - 518
EP  - 523
DO  - 10.1016/j.sjbs.2019.11.012
ER  - 

@article{
author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena",
year = "2020",
abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Journal of Biological Sciences",
title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia",
volume = "27",
number = "1",
pages = "518-523",
doi = "10.1016/j.sjbs.2019.11.012"
}

Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences
Elsevier, Amsterdam., 27(1), 518-523.
https://doi.org/10.1016/j.sjbs.2019.11.012

Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523.
doi:10.1016/j.sjbs.2019.11.012 .

Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523,
https://doi.org/10.1016/j.sjbs.2019.11.012 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Mišić, Dušan
AU  - Karabasil, Nedjeljko
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Jevtić, Goran
AU  - Ćirić, Jelena
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1756
AB  - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Clostridium botulinum spores in European honey bees from Serbia
VL  - 58
IS  - 3
SP  - 420
EP  - 426
DO  - 10.1080/00218839.2018.1560654
ER  - 

@article{
author = "Matović, Kazimir and Mišić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena",
year = "2019",
abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Clostridium botulinum spores in European honey bees from Serbia",
volume = "58",
number = "3",
pages = "420-426",
doi = "10.1080/00218839.2018.1560654"
}

Matović, K., Mišić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 58(3), 420-426.
https://doi.org/10.1080/00218839.2018.1560654

Matović K, Mišić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426.
doi:10.1080/00218839.2018.1560654 .

Matović, Kazimir, Mišić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426,
https://doi.org/10.1080/00218839.2018.1560654 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3337
AB  - У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима.
AB  - In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Prikaz slučaja mononuklearnog dermatitisa fazana
T1  - Case report: Mononuclear dermatitis in pheasant
SP  - 164
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3337
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2019",
abstract = "У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима., In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Prikaz slučaja mononuklearnog dermatitisa fazana, Case report: Mononuclear dermatitis in pheasant",
pages = "164-165",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3337"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2019). Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja mononuklearnog dermatitisa fazana" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):164-165,
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

TY  - JOUR
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1697
AB  - Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia
VL  - 69
IS  - 4
SP  - 369
EP  - 378
DO  - 10.2478/acve-2019-0031
ER  - 

@article{
author = "Vasković, Nikola and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Matović, Kazimir and Aničić, Milan and Marinković, Darko",
year = "2019",
abstract = "Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia",
volume = "69",
number = "4",
pages = "369-378",
doi = "10.2478/acve-2019-0031"
}

Vasković, N., Debeljak, Z., Vidanović, D., Šekler, M., Matović, K., Aničić, M.,& Marinković, D.. (2019). Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 369-378.
https://doi.org/10.2478/acve-2019-0031

Vasković N, Debeljak Z, Vidanović D, Šekler M, Matović K, Aničić M, Marinković D. Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd. 2019;69(4):369-378.
doi:10.2478/acve-2019-0031 .

Vasković, Nikola, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Matović, Kazimir, Aničić, Milan, Marinković, Darko, "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia" in Acta Veterinaria-Beograd, 69, no. 4 (2019):369-378,
https://doi.org/10.2478/acve-2019-0031 . .

TY  - CONF
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Sarić, Ljubiša
AU  - Karabasil, Nedjeljko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1677
AB  - The aim of this paper was to evaluate two real-time PCR (qPCR) protocols for the detection of Salmonella spp. in minced meat and chicken neck skin, after DNA extraction using the InstaTM Gene matrix (BioRad, USA) and DNA extraction based on thermal cell lysis. The applied molecular methods were sensitive and specific for the rapid detection of Salmonella spp. in minced meat and chicken neck skin. The qualitative results were identical regardless of the applied DNA extraction or qPCR protocols. Lower Cq values were achieved after DNA extraction using the InstaTM Gene matrix.
PB  - Iop Publishing Ltd, Bristol
C3  - 60th International Meat Industry Conference (MEATCON2019)
T1  - Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures
VL  - 333
SP  - UNSP 012041
DO  - 10.1088/1755-1315/333/1/012041
ER  - 

@conference{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Sarić, Ljubiša and Karabasil, Nedjeljko",
year = "2019",
abstract = "The aim of this paper was to evaluate two real-time PCR (qPCR) protocols for the detection of Salmonella spp. in minced meat and chicken neck skin, after DNA extraction using the InstaTM Gene matrix (BioRad, USA) and DNA extraction based on thermal cell lysis. The applied molecular methods were sensitive and specific for the rapid detection of Salmonella spp. in minced meat and chicken neck skin. The qualitative results were identical regardless of the applied DNA extraction or qPCR protocols. Lower Cq values were achieved after DNA extraction using the InstaTM Gene matrix.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "60th International Meat Industry Conference (MEATCON2019)",
title = "Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures",
volume = "333",
pages = "UNSP 012041",
doi = "10.1088/1755-1315/333/1/012041"
}

Dmitrić, M., Vidanović, D., Matović, K., Sarić, L.,& Karabasil, N.. (2019). Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures. in 60th International Meat Industry Conference (MEATCON2019)
Iop Publishing Ltd, Bristol., 333, UNSP 012041.
https://doi.org/10.1088/1755-1315/333/1/012041

Dmitrić M, Vidanović D, Matović K, Sarić L, Karabasil N. Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures. in 60th International Meat Industry Conference (MEATCON2019). 2019;333:UNSP 012041.
doi:10.1088/1755-1315/333/1/012041 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Sarić, Ljubiša, Karabasil, Nedjeljko, "Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures" in 60th International Meat Industry Conference (MEATCON2019), 333 (2019):UNSP 012041,
https://doi.org/10.1088/1755-1315/333/1/012041 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Ćirić, Jelena
AU  - Kaljević, Vesna
AU  - Nedić, Nebojša
AU  - Jevtić, Goran
AU  - Vasković, Nikola
AU  - Baltić, Milan Ž.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1660
AB  - Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia
VL  - 25
IS  - 14
SP  - 14148
EP  - 14157
DO  - 10.1007/s11356-018-1659-1
ER  - 

@article{
author = "Matović, Kazimir and Ćirić, Jelena and Kaljević, Vesna and Nedić, Nebojša and Jevtić, Goran and Vasković, Nikola and Baltić, Milan Ž.",
year = "2018",
abstract = "Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia",
volume = "25",
number = "14",
pages = "14148-14157",
doi = "10.1007/s11356-018-1659-1"
}

Matović, K., Ćirić, J., Kaljević, V., Nedić, N., Jevtić, G., Vasković, N.,& Baltić, M. Ž.. (2018). Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 25(14), 14148-14157.
https://doi.org/10.1007/s11356-018-1659-1

Matović K, Ćirić J, Kaljević V, Nedić N, Jevtić G, Vasković N, Baltić MŽ. Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research. 2018;25(14):14148-14157.
doi:10.1007/s11356-018-1659-1 .

Matović, Kazimir, Ćirić, Jelena, Kaljević, Vesna, Nedić, Nebojša, Jevtić, Goran, Vasković, Nikola, Baltić, Milan Ž., "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia" in Environmental Science and Pollution Research, 25, no. 14 (2018):14148-14157,
https://doi.org/10.1007/s11356-018-1659-1 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1581
AB  - After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.
PB  - Allen Press Inc, Lawrence
T2  - Journal of Parasitology
T1  - Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia
VL  - 104
IS  - 5
SP  - 557
EP  - 559
DO  - 10.1645/18-42
ER  - 

@article{
author = "Dmitrić, Marko and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Vasković, Nikola and Matović, Kazimir and Karabasil, Nedjeljko",
year = "2018",
abstract = "After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.",
publisher = "Allen Press Inc, Lawrence",
journal = "Journal of Parasitology",
title = "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia",
volume = "104",
number = "5",
pages = "557-559",
doi = "10.1645/18-42"
}

Dmitrić, M., Debeljak, Z., Vidanović, D., Šekler, M., Vasković, N., Matović, K.,& Karabasil, N.. (2018). Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology
Allen Press Inc, Lawrence., 104(5), 557-559.
https://doi.org/10.1645/18-42

Dmitrić M, Debeljak Z, Vidanović D, Šekler M, Vasković N, Matović K, Karabasil N. Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology. 2018;104(5):557-559.
doi:10.1645/18-42 .

Dmitrić, Marko, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Vasković, Nikola, Matović, Kazimir, Karabasil, Nedjeljko, "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia" in Journal of Parasitology, 104, no. 5 (2018):557-559,
https://doi.org/10.1645/18-42 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Sarić, Ljubiša
AU  - Arsić, Milos
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1641
AB  - The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.
PB  - Wiley, Hoboken
T2  - Journal of Food Processing and Preservation
T1  - In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food
VL  - 42
IS  - 2
SP  - e13455
DO  - 10.1111/jfpp.13455
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Šekler, Milanko and Sarić, Ljubiša and Arsić, Milos and Karabasil, Nedjeljko",
year = "2018",
abstract = "The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Processing and Preservation",
title = "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food",
volume = "42",
number = "2",
pages = "e13455",
doi = "10.1111/jfpp.13455"
}

Dmitrić, M., Vidanović, D., Matović, K., Šekler, M., Sarić, L., Arsić, M.,& Karabasil, N.. (2018). In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation
Wiley, Hoboken., 42(2), e13455.
https://doi.org/10.1111/jfpp.13455

Dmitrić M, Vidanović D, Matović K, Šekler M, Sarić L, Arsić M, Karabasil N. In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation. 2018;42(2):e13455.
doi:10.1111/jfpp.13455 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Šekler, Milanko, Sarić, Ljubiša, Arsić, Milos, Karabasil, Nedjeljko, "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food" in Journal of Food Processing and Preservation, 42, no. 2 (2018):e13455,
https://doi.org/10.1111/jfpp.13455 . .

TY  - JOUR
AU  - Stević, Nataša
AU  - Mišić, Dušan
AU  - Bogunović, Danica
AU  - Matović, Kazimir
AU  - Valčić, Miroslav
AU  - Milovanović, Milovan
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1544
AB  - The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay
VL  - 67
IS  - 4
SP  - 551
EP  - 561
DO  - 10.1515/acve-2017-0046
ER  - 

@article{
author = "Stević, Nataša and Mišić, Dušan and Bogunović, Danica and Matović, Kazimir and Valčić, Miroslav and Milovanović, Milovan and Radojičić, Sonja",
year = "2017",
abstract = "The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay",
volume = "67",
number = "4",
pages = "551-561",
doi = "10.1515/acve-2017-0046"
}

Stević, N., Mišić, D., Bogunović, D., Matović, K., Valčić, M., Milovanović, M.,& Radojičić, S.. (2017). Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 551-561.
https://doi.org/10.1515/acve-2017-0046

Stević N, Mišić D, Bogunović D, Matović K, Valčić M, Milovanović M, Radojičić S. Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd. 2017;67(4):551-561.
doi:10.1515/acve-2017-0046 .

Stević, Nataša, Mišić, Dušan, Bogunović, Danica, Matović, Kazimir, Valčić, Miroslav, Milovanović, Milovan, Radojičić, Sonja, "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay" in Acta Veterinaria-Beograd, 67, no. 4 (2017):551-561,
https://doi.org/10.1515/acve-2017-0046 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Debeljak, Zoran
AU  - Karabasil, Nedjeljko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1483
AB  - Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.
PB  - Amer Assoc Zoo Veterinarians, Yulee
T2  - Journal of Zoo and Wildlife Medicine
T1  - Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia
VL  - 48
IS  - 3
SP  - 703
EP  - 707
DO  - 10.1638/2016-0169.1
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Vasković, Nikola and Matović, Kazimir and Šekler, Milanko and Debeljak, Zoran and Karabasil, Nedjeljko",
year = "2017",
abstract = "Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.",
publisher = "Amer Assoc Zoo Veterinarians, Yulee",
journal = "Journal of Zoo and Wildlife Medicine",
title = "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia",
volume = "48",
number = "3",
pages = "703-707",
doi = "10.1638/2016-0169.1"
}

Dmitrić, M., Vidanović, D., Vasković, N., Matović, K., Šekler, M., Debeljak, Z.,& Karabasil, N.. (2017). Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine
Amer Assoc Zoo Veterinarians, Yulee., 48(3), 703-707.
https://doi.org/10.1638/2016-0169.1

Dmitrić M, Vidanović D, Vasković N, Matović K, Šekler M, Debeljak Z, Karabasil N. Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine. 2017;48(3):703-707.
doi:10.1638/2016-0169.1 .

Dmitrić, Marko, Vidanović, Dejan, Vasković, Nikola, Matović, Kazimir, Šekler, Milanko, Debeljak, Zoran, Karabasil, Nedjeljko, "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia" in Journal of Zoo and Wildlife Medicine, 48, no. 3 (2017):703-707,
https://doi.org/10.1638/2016-0169.1 . .

TY  - CONF
AU  - Matović, Kazimir
AU  - Baltić, Milan Ž.
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Nenadić, Dragan
AU  - Vasković, Nikola
AU  - Jevtić, Goran
AU  - Mišić, Dušan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1267
AB  - The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.
PB  - Elsevier Science Bv, Amsterdam
C3  - 58th International Meat Industry Conference (MEATCON2015)
T1  - The investigation of the presence of Clostridium botulinum spores in honey in Serbia
VL  - 5
SP  - 180
EP  - 183
DO  - 10.1016/j.profoo.2015.09.051
ER  - 

@conference{
author = "Matović, Kazimir and Baltić, Milan Ž. and Nedić, Nebojša and Dmitrić, Marko and Nenadić, Dragan and Vasković, Nikola and Jevtić, Goran and Mišić, Dušan",
year = "2015",
abstract = "The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "58th International Meat Industry Conference (MEATCON2015)",
title = "The investigation of the presence of Clostridium botulinum spores in honey in Serbia",
volume = "5",
pages = "180-183",
doi = "10.1016/j.profoo.2015.09.051"
}

Matović, K., Baltić, M. Ž., Nedić, N., Dmitrić, M., Nenadić, D., Vasković, N., Jevtić, G.,& Mišić, D.. (2015). The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015)
Elsevier Science Bv, Amsterdam., 5, 180-183.
https://doi.org/10.1016/j.profoo.2015.09.051

Matović K, Baltić MŽ, Nedić N, Dmitrić M, Nenadić D, Vasković N, Jevtić G, Mišić D. The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015). 2015;5:180-183.
doi:10.1016/j.profoo.2015.09.051 .

Matović, Kazimir, Baltić, Milan Ž., Nedić, Nebojša, Dmitrić, Marko, Nenadić, Dragan, Vasković, Nikola, Jevtić, Goran, Mišić, Dušan, "The investigation of the presence of Clostridium botulinum spores in honey in Serbia" in 58th International Meat Industry Conference (MEATCON2015), 5 (2015):180-183,
https://doi.org/10.1016/j.profoo.2015.09.051 . .

TY  - CONF
AU  - Matović, Kazimir
AU  - Baltić, Milan Ž.
AU  - Mišić, Dušan
AU  - Nedić, Nebojša
AU  - Karabasil, Nedjeljko
AU  - Ranin, Lazar
AU  - Ivanović, Jelena
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3750
AB  - U cilju kvalitativno kvantitativnog utvrđivanja prisustva mikroorganizama, u uzorcima meda, koriste se klasiče i međunarodno priznate, standardne (ISO) metode izolacije, identifikacije, kvantifikacije i determinacije.
Početkom dvadesetprvog veka a u cilju dobijanja brzih i pouzdanih rezultata laboratoriskih ispitivanja pristupa se izvođenju molekularnih metoda u uvrđivanju prisustva mikroorganizama u uzorcima meda. Navedene metode koristile su se za potvrdu i determinaciju mikroorganizama već izolovanih klasičnim i standardnim mikrobiološkim metodama ali i za direktno utvrđivanje prisustva mikroorganizama u ispitivanim uzorcima. U navedena ispitivanja uključena su ponovljena ispitivanja zbog fizičko-hemijskih karakteristika meda odnosno neravnomerne raspoređenosti mikroorganizama u ispitivanim uzorcima i mogućnosti dobijanja lažno negativnih rezultata. Molekularni pristupi u dijagnostici uključivali su osetljive i specifične testove lančane reakcije polimeraze (PCR), lančane reakcije polimeraze u realnom vremenu (Real-Time PCR) i metode gel-elektroforeze u pulsirajućem električnom polju (Pulse-Field Gel Elektrophoresis-PFGE). Detekcija mikroorganizama direktno iz neobrađenih uzoraka meda nije moguća. Zbog prisustva malog broja mikroorganizama, uglavnom spora, neophodna je priprema uzoraka meda koja mora uključiti postupak dilucije, predobogaćenja, centrifugovanja i membranske filtracije. Ovako dobijeni sadržaj koristi se za izolaciju ciljanih mikroorganizama klasičnim i standardnim metodama laboratorijske dijagnostike, odnosno za prečišćavanje i ekstrakciju nukleinskih kiselina za molekularne metode dijagnostike. U cilju detekcije mikroorganizama, u uzorcima meda, primenom molekularnih metoda dijagnostike, za svaki ispitujući uzorak moraju se izvoditi višestruka ponovljena ispitivanja zbog neravnomerne raspoređenosti mikroorganizama/spora i mogućnosti dobijanja lažno negativnih rezultata.
U uzorcima meda kontaminiranim referentnim sojevima mikroorganizama, molekularnim metodama može da se dokaže prisustvo jedne ćelije/spore mikroorganizma u jednom gramu meda dok je to nemoguće dokazati klasičnim/standardnim metodama. Klasične i standardne metode mikrobiološke dijagnostike nisu podesne za detekciju mikroorganizama u uzorcima meda jer ove metode zbog niske osetljivosti daju lažno negativne rezultate.
PB  - Beograd : Fakultet veterinarske medicine
C3  - 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014
T1  - Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda
SP  - 90
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3750
ER  - 

@conference{
author = "Matović, Kazimir and Baltić, Milan Ž. and Mišić, Dušan and Nedić, Nebojša and Karabasil, Nedjeljko and Ranin, Lazar and Ivanović, Jelena",
year = "2014",
abstract = "U cilju kvalitativno kvantitativnog utvrđivanja prisustva mikroorganizama, u uzorcima meda, koriste se klasiče i međunarodno priznate, standardne (ISO) metode izolacije, identifikacije, kvantifikacije i determinacije.
Početkom dvadesetprvog veka a u cilju dobijanja brzih i pouzdanih rezultata laboratoriskih ispitivanja pristupa se izvođenju molekularnih metoda u uvrđivanju prisustva mikroorganizama u uzorcima meda. Navedene metode koristile su se za potvrdu i determinaciju mikroorganizama već izolovanih klasičnim i standardnim mikrobiološkim metodama ali i za direktno utvrđivanje prisustva mikroorganizama u ispitivanim uzorcima. U navedena ispitivanja uključena su ponovljena ispitivanja zbog fizičko-hemijskih karakteristika meda odnosno neravnomerne raspoređenosti mikroorganizama u ispitivanim uzorcima i mogućnosti dobijanja lažno negativnih rezultata. Molekularni pristupi u dijagnostici uključivali su osetljive i specifične testove lančane reakcije polimeraze (PCR), lančane reakcije polimeraze u realnom vremenu (Real-Time PCR) i metode gel-elektroforeze u pulsirajućem električnom polju (Pulse-Field Gel Elektrophoresis-PFGE). Detekcija mikroorganizama direktno iz neobrađenih uzoraka meda nije moguća. Zbog prisustva malog broja mikroorganizama, uglavnom spora, neophodna je priprema uzoraka meda koja mora uključiti postupak dilucije, predobogaćenja, centrifugovanja i membranske filtracije. Ovako dobijeni sadržaj koristi se za izolaciju ciljanih mikroorganizama klasičnim i standardnim metodama laboratorijske dijagnostike, odnosno za prečišćavanje i ekstrakciju nukleinskih kiselina za molekularne metode dijagnostike. U cilju detekcije mikroorganizama, u uzorcima meda, primenom molekularnih metoda dijagnostike, za svaki ispitujući uzorak moraju se izvoditi višestruka ponovljena ispitivanja zbog neravnomerne raspoređenosti mikroorganizama/spora i mogućnosti dobijanja lažno negativnih rezultata.
U uzorcima meda kontaminiranim referentnim sojevima mikroorganizama, molekularnim metodama može da se dokaže prisustvo jedne ćelije/spore mikroorganizma u jednom gramu meda dok je to nemoguće dokazati klasičnim/standardnim metodama. Klasične i standardne metode mikrobiološke dijagnostike nisu podesne za detekciju mikroorganizama u uzorcima meda jer ove metode zbog niske osetljivosti daju lažno negativne rezultate.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014",
title = "Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda",
pages = "90-102",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3750"
}

Matović, K., Baltić, M. Ž., Mišić, D., Nedić, N., Karabasil, N., Ranin, L.,& Ivanović, J.. (2014). Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda. in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014
Beograd : Fakultet veterinarske medicine., 90-102.
https://hdl.handle.net/21.15107/rcub_veterinar_3750

Matović K, Baltić MŽ, Mišić D, Nedić N, Karabasil N, Ranin L, Ivanović J. Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda. in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014. 2014;:90-102.
https://hdl.handle.net/21.15107/rcub_veterinar_3750 .

Matović, Kazimir, Baltić, Milan Ž., Mišić, Dušan, Nedić, Nebojša, Karabasil, Nedjeljko, Ranin, Lazar, Ivanović, Jelena, "Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda" in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014 (2014):90-102,
https://hdl.handle.net/21.15107/rcub_veterinar_3750 .

TY  - THES
AU  - Matović, Kazimir
PY  - 2013
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44863503
UR  - http://nardus.mpn.gov.rs/123456789/2975
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/23
AB  - In this study, the presence of Clostridium botulinum spores in different types of honey and honey bees was examined. The sum of 59 honey and 61 honey bee samples was included in this investigation and samples originated from different regions of the Republic of Serbia. In addition to classical microbiological methods, after the dilution, enrichment, spinning and membrane filtration, PCR and multiplex PCR methods were used. Determination the number of spores in the PCR positive samples, was done using the most likely possible number (MPN) methodology. According to the results obtained by conventional microbiological methods and multiplex PCR, the presence of Clostridium botulinum spores, was detected in 5 honey samples (8.47%) and 1 (1.64%) honey bee sample. Number of spores in positive honey samples was from 20/kg to 204/kg, and in a sample of honey bees was 110/kg. In one honey sample, spores of type B C. botulinum were detected, in one honey sample spores of type E, in two honey samples spores of types A and E, and in one sample spores of three types of C. botulinum (A, B, E) were detected. Type E Clostridium botulinum spores were detected in a sample of honey bees. In the same samples of honey or honey bees, using conventional qualitative and quantitative microbiological methods, spores of Clostridium botulinum were not detected. The detection of Clostridium botulinum spores with PCR, multiplex PCR and conventional microbiological methods in honey and honey bee samples is not possible without preenrichment. Conventional microbiological methods, including SRPS ISO 15213:2011, are not suitable for the detection of Clostridium botulinum spores in honey samples. Due to the low sensitivity of these methods, they can lead to false negative results. In the honey samples which served as a positive control and wich were contaminated with Clostridium botulinum NCTC 7272 strain, the PCR method may prove a presence of one spore/g of honey. In order to detect Clostridium botulinum spores in honey and honey bees samples using PCR method, due to the small and/or unequal distribution of spores in the samples, the test had to be repeated at least three times for each sample.
AB  - U ovom radu vršeno je ispitivanje prisustva spora Clostridium botulinum u uzorcima razlicitih vrsta meda i medonosnih pcela. Ispitano je ukupno 59 uzoraka meda i 61 uzorak medonosnih pcela, poreklom iz razlicitih regona Republike Srbije. Pored klasicnih mikrobioloških metoda u laboratorijskim ispitivanjima je posle postupka dilucije, predobogacenja, centrifugovanja i membranske filtracije primenjena metoda multipleks PCR i PCR. Odredivanje broja spora, u PCR pozitivnim uzorcima, radeno je metodom najverovatnijeg moguceg broja (MPN). Prisustvo spora Clostridium botulinum, metodom multipleks PCR i PCR-a, utvrdeno je u 5 uzoraka (8,47%) meda i jednom (1,64%) uzorku medonosnih pcela. Broj spora u pozitivnim uzorcima meda bio je od 20/kg do 204/kg, a u uzorku medonosnih pcela 110/kg. U jednom uzorku meda detektovane su spore B tipa C. botulinum, u jednom uzorku je detektovan E tip, u dva uzorka su detektovane spore istovremeno dva tipa C.botulinum (A, E) i u jednom uzorku istovremeno tri tipa C. botulinum (A, B, E). Spore Clostridium botulinum tipa E dokazane su u jednom uzorku medonosnih pcela. U istim uzorcima meda, odnosno medonosnih pcela, klasicnim kvalitativno-kvantitativnim metodama nije dokazano prisustvo spora Clostridium botulinum. Detekcija spora Clostridium botulinum direktno iz neobradenih uzoraka meda i medonosnih pcela, bez predobogacenja, nije moguca primenom metoda PCR i klasicnih metoda mikrobiologije. Klasicne metode mikrobiologije, ukljucujuci i metodu SRPS ISO 15213:2011, nisu podesne za detekciju spora Clostridium botulinum u uzorcima meda. Zbog niske osetljivosti navedene metode daju lažno negativne rezultate. U uzorku meda koji je služio kao pozitivna kontrola i koji je prethodno namerno kontaminiran referentnim sojem Clostridium botulinum NCTC 7272, PCR metodom može da se dokaže jedna spora/g meda. U cilju detekcije spora Clostridium botulinum u uzorcima meda i medonosnih pcela primenom metode PCR, za svaki ispitujuci uzorak moraju se izvoditi višestruka ponovljanja, ispitivanja jednog istog uzorka, zbog malog broja i/ili neravnomerne rasporedenosti spora u uzorcima.
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - The investigation of the presence of Clostridium botulinum in honey and honey bee samples
T1  - Utvrđivanje prisustva Clostridium botulinum u uzorcima meda i medonosnih pčela
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2975
ER  - 

@phdthesis{
author = "Matović, Kazimir",
year = "2013",
abstract = "In this study, the presence of Clostridium botulinum spores in different types of honey and honey bees was examined. The sum of 59 honey and 61 honey bee samples was included in this investigation and samples originated from different regions of the Republic of Serbia. In addition to classical microbiological methods, after the dilution, enrichment, spinning and membrane filtration, PCR and multiplex PCR methods were used. Determination the number of spores in the PCR positive samples, was done using the most likely possible number (MPN) methodology. According to the results obtained by conventional microbiological methods and multiplex PCR, the presence of Clostridium botulinum spores, was detected in 5 honey samples (8.47%) and 1 (1.64%) honey bee sample. Number of spores in positive honey samples was from 20/kg to 204/kg, and in a sample of honey bees was 110/kg. In one honey sample, spores of type B C. botulinum were detected, in one honey sample spores of type E, in two honey samples spores of types A and E, and in one sample spores of three types of C. botulinum (A, B, E) were detected. Type E Clostridium botulinum spores were detected in a sample of honey bees. In the same samples of honey or honey bees, using conventional qualitative and quantitative microbiological methods, spores of Clostridium botulinum were not detected. The detection of Clostridium botulinum spores with PCR, multiplex PCR and conventional microbiological methods in honey and honey bee samples is not possible without preenrichment. Conventional microbiological methods, including SRPS ISO 15213:2011, are not suitable for the detection of Clostridium botulinum spores in honey samples. Due to the low sensitivity of these methods, they can lead to false negative results. In the honey samples which served as a positive control and wich were contaminated with Clostridium botulinum NCTC 7272 strain, the PCR method may prove a presence of one spore/g of honey. In order to detect Clostridium botulinum spores in honey and honey bees samples using PCR method, due to the small and/or unequal distribution of spores in the samples, the test had to be repeated at least three times for each sample., U ovom radu vršeno je ispitivanje prisustva spora Clostridium botulinum u uzorcima razlicitih vrsta meda i medonosnih pcela. Ispitano je ukupno 59 uzoraka meda i 61 uzorak medonosnih pcela, poreklom iz razlicitih regona Republike Srbije. Pored klasicnih mikrobioloških metoda u laboratorijskim ispitivanjima je posle postupka dilucije, predobogacenja, centrifugovanja i membranske filtracije primenjena metoda multipleks PCR i PCR. Odredivanje broja spora, u PCR pozitivnim uzorcima, radeno je metodom najverovatnijeg moguceg broja (MPN). Prisustvo spora Clostridium botulinum, metodom multipleks PCR i PCR-a, utvrdeno je u 5 uzoraka (8,47%) meda i jednom (1,64%) uzorku medonosnih pcela. Broj spora u pozitivnim uzorcima meda bio je od 20/kg do 204/kg, a u uzorku medonosnih pcela 110/kg. U jednom uzorku meda detektovane su spore B tipa C. botulinum, u jednom uzorku je detektovan E tip, u dva uzorka su detektovane spore istovremeno dva tipa C.botulinum (A, E) i u jednom uzorku istovremeno tri tipa C. botulinum (A, B, E). Spore Clostridium botulinum tipa E dokazane su u jednom uzorku medonosnih pcela. U istim uzorcima meda, odnosno medonosnih pcela, klasicnim kvalitativno-kvantitativnim metodama nije dokazano prisustvo spora Clostridium botulinum. Detekcija spora Clostridium botulinum direktno iz neobradenih uzoraka meda i medonosnih pcela, bez predobogacenja, nije moguca primenom metoda PCR i klasicnih metoda mikrobiologije. Klasicne metode mikrobiologije, ukljucujuci i metodu SRPS ISO 15213:2011, nisu podesne za detekciju spora Clostridium botulinum u uzorcima meda. Zbog niske osetljivosti navedene metode daju lažno negativne rezultate. U uzorku meda koji je služio kao pozitivna kontrola i koji je prethodno namerno kontaminiran referentnim sojem Clostridium botulinum NCTC 7272, PCR metodom može da se dokaže jedna spora/g meda. U cilju detekcije spora Clostridium botulinum u uzorcima meda i medonosnih pcela primenom metode PCR, za svaki ispitujuci uzorak moraju se izvoditi višestruka ponovljanja, ispitivanja jednog istog uzorka, zbog malog broja i/ili neravnomerne rasporedenosti spora u uzorcima.",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "The investigation of the presence of Clostridium botulinum in honey and honey bee samples, Utvrđivanje prisustva Clostridium botulinum u uzorcima meda i medonosnih pčela",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2975"
}

Matović, K.. (2013). The investigation of the presence of Clostridium botulinum in honey and honey bee samples. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_2975

Matović K. The investigation of the presence of Clostridium botulinum in honey and honey bee samples. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_2975 .

Matović, Kazimir, "The investigation of the presence of Clostridium botulinum in honey and honey bee samples" (2013),
https://hdl.handle.net/21.15107/rcub_nardus_2975 .

TY  - JOUR
AU  - Vasković, Nikola
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Polaček, Vladimir
AU  - Kukolj, Vladimir
AU  - Matović, Kazimir
AU  - Jovanović, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/803
AB  - The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined.
AB  - Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus
T1  - Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence
VL  - 61
IS  - 5-6
SP  - 591
EP  - 598
DO  - 10.2298/AVB1106591V
ER  - 

@article{
author = "Vasković, Nikola and Šekler, Milanko and Vidanović, Dejan and Polaček, Vladimir and Kukolj, Vladimir and Matović, Kazimir and Jovanović, M.",
year = "2011",
abstract = "The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined., Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus, Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence",
volume = "61",
number = "5-6",
pages = "591-598",
doi = "10.2298/AVB1106591V"
}

Vasković, N., Šekler, M., Vidanović, D., Polaček, V., Kukolj, V., Matović, K.,& Jovanović, M.. (2011). Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 591-598.
https://doi.org/10.2298/AVB1106591V

Vasković N, Šekler M, Vidanović D, Polaček V, Kukolj V, Matović K, Jovanović M. Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd. 2011;61(5-6):591-598.
doi:10.2298/AVB1106591V .

Vasković, Nikola, Šekler, Milanko, Vidanović, Dejan, Polaček, Vladimir, Kukolj, Vladimir, Matović, Kazimir, Jovanović, M., "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus" in Acta Veterinaria-Beograd, 61, no. 5-6 (2011):591-598,
https://doi.org/10.2298/AVB1106591V . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Šekler, Marina
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/580
AB  - Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.
AB  - Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry
T1  - Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine
VL  - 63
IS  - 1-2
SP  - 37
EP  - 44
DO  - 10.2298/VETGL0902037M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Radojičić, Marina and Šekler, Marina and Matović, Kazimir and Vidanović, Dejan",
year = "2009",
abstract = "Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32., Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry, Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine",
volume = "63",
number = "1-2",
pages = "37-44",
doi = "10.2298/VETGL0902037M"
}

Milić, N., Nišavić, J., Radojičić, M., Šekler, M., Matović, K.,& Vidanović, D.. (2009). Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 37-44.
https://doi.org/10.2298/VETGL0902037M

Milić N, Nišavić J, Radojičić M, Šekler M, Matović K, Vidanović D. Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik. 2009;63(1-2):37-44.
doi:10.2298/VETGL0902037M .

Milić, Nenad, Nišavić, Jakov, Radojičić, Marina, Šekler, Marina, Matović, Kazimir, Vidanović, Dejan, "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry" in Veterinarski Glasnik, 63, no. 1-2 (2009):37-44,
https://doi.org/10.2298/VETGL0902037M . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Ašanin, Ružica
AU  - Radojičić, Sonja
AU  - Lako, Branislav
AU  - Žarković, Aleksandar
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/564
AB  - The most reliable diagnosis of an infectuous disease is confirmed by isolation of its pathogen. When it comes to brucellosis, it is important to know that brucella isolation is rarely successful; it is not only very complicated but is as well hazardous for laboratory workers. Due to the above mentioned reasons, it is reasonable to use serological tests for routine diagnosis of this zoonose. This paper deals with examination of bovine sera samples with the aim to detect the titer of specific antibodies against brucellosis. In order to choose and evaluate properly the best test in terms of applicability, speed of performance, and provision of correct results, five serological tests were assayed: rapid serum plate agglutination (Rose Bengal test), Brucella abortus bovis test (RB, BAB test); serum agglutination test (titration) - by Wright, as micro method (mSAT); reaction of complement fixation, and also as micro method (mCF); indirect imunoenzyime test (iELISA) and competitive imunoenzyme test (cELISA). This paper includes 630 samples of bovine blood sera, as well as positive and negative international antibrucella serum as the mandatory control. The presence of specific antibodies against brucella was determined in 125 samples of bovine blood sera. Based on the analysis of the results obtained, evaluation of sensitivity and specificity of these tests was conducted. iELISA and RB test proved to be the most sensitive, while the highest specificity was determined in mCF, and less specific were mSAT and iELISA. RB test had the lowest specificity.
AB  - Najsigurnija dijagnostika bolesti infektivnog karaktera vrši se izolacijom uzroč nika. Kada je bruceloza u pitanju, mora da se ima u vidu, da je izolacija brucela uspešna u niskom procentu, veoma komplikovana i predstavlja opasnost po laboratorijske radnike. Zbog navedenih razloga, primena seroloških testova u rutinskoj dijagnostici ove zoonoze je opravdana. U ovom radu su vršena ispitivanja uzoraka seruma goveda radi određivanja prisustva titra specifičnih antitela protiv brucela, u svrhu dijagnostikovanja bruceloze. U nameri da se pravilno izbabere i proceni najbolji test u smislu aplikativnosti, brzine izvođenja i dobijanja pouzdanih rezultata, tokom ispitivanja je korišćeno pet seroloških testova: brza serumska aglutinacija na pločici - Rose Bengal test - Brucella abortus bovis test (RB, BAB test); spora aglutinacija (u titraciji) - metoda po Wrightu, kao mikrometoda (mSAT); reakcija vezivanja komplementa, takođe kao mikrometoda (mRVK); indirektni imunoenzimski test (iELISA) i kompetitivni imunoenzimski test (cELISA). Ispitivanjem je bilo obuhvaćeno 630 uzoraka krvnog seruma goveda kao i pozitivni i negativni internacionalni antibrucela serumi kao obavezne kontrole. Prisustvo specifičnih antitela protiv brucela vrsta ustanovljeno je u 125 uzoraka krvnog seruma goveda. Na osnovu analize dobijenih rezultata vršena je procena osetljivosti i specifičnosti navedenih testova. Najosetljivijim su se pokazali iELISA i BAB test, a najveća specifičnost je ustanovljena kod mRVK. Manje specifični bili su mSAT i iELISA, a najmanja specifičnost je ustanovljena kod BAB testa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis
T1  - Ispitivanje osetljivosti i specifičnosti nekih seroloških testova u dijagnostici bruceloze kod goveda
VL  - 58
IS  - 5-6
SP  - 467
EP  - 476
DO  - 10.2298/AVB0806467M
ER  - 

@article{
author = "Matović, Kazimir and Ašanin, Ružica and Radojičić, Sonja and Lako, Branislav and Žarković, Aleksandar",
year = "2008",
abstract = "The most reliable diagnosis of an infectuous disease is confirmed by isolation of its pathogen. When it comes to brucellosis, it is important to know that brucella isolation is rarely successful; it is not only very complicated but is as well hazardous for laboratory workers. Due to the above mentioned reasons, it is reasonable to use serological tests for routine diagnosis of this zoonose. This paper deals with examination of bovine sera samples with the aim to detect the titer of specific antibodies against brucellosis. In order to choose and evaluate properly the best test in terms of applicability, speed of performance, and provision of correct results, five serological tests were assayed: rapid serum plate agglutination (Rose Bengal test), Brucella abortus bovis test (RB, BAB test); serum agglutination test (titration) - by Wright, as micro method (mSAT); reaction of complement fixation, and also as micro method (mCF); indirect imunoenzyime test (iELISA) and competitive imunoenzyme test (cELISA). This paper includes 630 samples of bovine blood sera, as well as positive and negative international antibrucella serum as the mandatory control. The presence of specific antibodies against brucella was determined in 125 samples of bovine blood sera. Based on the analysis of the results obtained, evaluation of sensitivity and specificity of these tests was conducted. iELISA and RB test proved to be the most sensitive, while the highest specificity was determined in mCF, and less specific were mSAT and iELISA. RB test had the lowest specificity., Najsigurnija dijagnostika bolesti infektivnog karaktera vrši se izolacijom uzroč nika. Kada je bruceloza u pitanju, mora da se ima u vidu, da je izolacija brucela uspešna u niskom procentu, veoma komplikovana i predstavlja opasnost po laboratorijske radnike. Zbog navedenih razloga, primena seroloških testova u rutinskoj dijagnostici ove zoonoze je opravdana. U ovom radu su vršena ispitivanja uzoraka seruma goveda radi određivanja prisustva titra specifičnih antitela protiv brucela, u svrhu dijagnostikovanja bruceloze. U nameri da se pravilno izbabere i proceni najbolji test u smislu aplikativnosti, brzine izvođenja i dobijanja pouzdanih rezultata, tokom ispitivanja je korišćeno pet seroloških testova: brza serumska aglutinacija na pločici - Rose Bengal test - Brucella abortus bovis test (RB, BAB test); spora aglutinacija (u titraciji) - metoda po Wrightu, kao mikrometoda (mSAT); reakcija vezivanja komplementa, takođe kao mikrometoda (mRVK); indirektni imunoenzimski test (iELISA) i kompetitivni imunoenzimski test (cELISA). Ispitivanjem je bilo obuhvaćeno 630 uzoraka krvnog seruma goveda kao i pozitivni i negativni internacionalni antibrucela serumi kao obavezne kontrole. Prisustvo specifičnih antitela protiv brucela vrsta ustanovljeno je u 125 uzoraka krvnog seruma goveda. Na osnovu analize dobijenih rezultata vršena je procena osetljivosti i specifičnosti navedenih testova. Najosetljivijim su se pokazali iELISA i BAB test, a najveća specifičnost je ustanovljena kod mRVK. Manje specifični bili su mSAT i iELISA, a najmanja specifičnost je ustanovljena kod BAB testa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis, Ispitivanje osetljivosti i specifičnosti nekih seroloških testova u dijagnostici bruceloze kod goveda",
volume = "58",
number = "5-6",
pages = "467-476",
doi = "10.2298/AVB0806467M"
}

Matović, K., Ašanin, R., Radojičić, S., Lako, B.,& Žarković, A.. (2008). Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 467-476.
https://doi.org/10.2298/AVB0806467M

Matović K, Ašanin R, Radojičić S, Lako B, Žarković A. Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis. in Acta Veterinaria-Beograd. 2008;58(5-6):467-476.
doi:10.2298/AVB0806467M .

Matović, Kazimir, Ašanin, Ružica, Radojičić, Sonja, Lako, Branislav, Žarković, Aleksandar, "Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):467-476,
https://doi.org/10.2298/AVB0806467M . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Vasković, Nikola
AU  - Žarković, Aleksandar
AU  - Matović, Kazimir
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/518
AB  - In addition to the implementation of standard methods of virological diagnostics used for the isolation of the Newcastle disease virus from suspect material, as well as for its identification, nowadays there is increasing use of molecular diagnostic methods, primarily reverse transcription polymerase chain reaction (RT-PCR) and the sequencing method. The objective of this work was to examine possibilities for the implementation of the above methods in the diagnosis of poultry infection caused by the Newcastle disease virus. The presence of hem agglutination antigens for the Newcastle disease virus was established in samples of allantoises liquid from 62 poultry embargoed eggs 72 h after inoculation, whose titers ranged from 1:16 to 1:2048, while the hem agglutination inhibition test (HI test) with the implementation of a referent immuno serum against the given cause provided the identification of isolated viruses in serum dilutions of 1:128 to 1:1024. The RT-PCR method and the PCR established that in eight examined samples one fragment each of viral RNA is formed in agars gel of a size of 254bp, which is characteristic for the Newcastle disease virus genome according to its nucleotide sequence. On the grounds of a comparative analysis of RNA sequences obtained from eight isolated NDV strains and the genome sequences of referent atypical poultry influenza viral strains using Mega 40 and BLAST programmers, it was established that the isolated strains of the Newcastle disease virus were highly virulent.
AB  - Pored primene standardnih metoda virusološke dijagnostike koje se koriste za izolovanje virusa Newcastle bolesti iz suspektnog materijala kao i za njegovu identifikaciju, danas su sve više u upotrebi i molekularne metode dijagnostike i to pre svega lančana reakcija polimeraze (RT-PCR) i metoda sekvenciranja. Cilj ovog rada bio je ispitivanje mogućnosti primene navedenih metoda u dijagnostici infekcije živine izazvane virusom Newcastle bolesti. Prisustvo hemaglutinacionih antigena virusa Newcastle bolesti ustanovljeno je u uzorcima alantoisne tečnosti od 62 embrionirana jaja živine posle 72h od inokulacije čiji su se titri kretali od 1:16 do 1:2048, dok je testom heminhibicije (HI testom) uz primenu referentnog imunog seruma protiv navedenog uzročnika izvršena identifikacija izolovanih virusa u razređenjima seruma od 1:128 do 1:1024. Metodom reverzne transkripcije (RT-PCT) i lančane reakcije polimeraze (PCR) ustanovljeno da se kod osam ispitivanih uzoraka formira po jedan fragment virusne RNK u gelu agaroze veličine od 254bp koji je po sekvenci nukleotida karakterističan za genom virusa Newcastle bolesti. Na osnovu uporedne analize sekvenci RNK dobijenih od osam izolovanih sojeva virusa NDV i sekvenci genoma referentnih sojeva virusa atipične kuge živine uz korišćenje Mega 40 i BLAST programa, utvrđeno je da su izolovani sojevi virusa Newcastle bolesti visoko virulentni.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics
T1  - Identifikacija izolovanih sojeva virusa atipične kuge živine primenom molekularnih metoda virusološke dijagnostike
VL  - 62
IS  - 3-4
SP  - 167
EP  - 177
DO  - 10.2298/VETGL0804167V
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Vasković, Nikola and Žarković, Aleksandar and Matović, Kazimir and Milić, Nenad and Nišavić, Jakov",
year = "2008",
abstract = "In addition to the implementation of standard methods of virological diagnostics used for the isolation of the Newcastle disease virus from suspect material, as well as for its identification, nowadays there is increasing use of molecular diagnostic methods, primarily reverse transcription polymerase chain reaction (RT-PCR) and the sequencing method. The objective of this work was to examine possibilities for the implementation of the above methods in the diagnosis of poultry infection caused by the Newcastle disease virus. The presence of hem agglutination antigens for the Newcastle disease virus was established in samples of allantoises liquid from 62 poultry embargoed eggs 72 h after inoculation, whose titers ranged from 1:16 to 1:2048, while the hem agglutination inhibition test (HI test) with the implementation of a referent immuno serum against the given cause provided the identification of isolated viruses in serum dilutions of 1:128 to 1:1024. The RT-PCR method and the PCR established that in eight examined samples one fragment each of viral RNA is formed in agars gel of a size of 254bp, which is characteristic for the Newcastle disease virus genome according to its nucleotide sequence. On the grounds of a comparative analysis of RNA sequences obtained from eight isolated NDV strains and the genome sequences of referent atypical poultry influenza viral strains using Mega 40 and BLAST programmers, it was established that the isolated strains of the Newcastle disease virus were highly virulent., Pored primene standardnih metoda virusološke dijagnostike koje se koriste za izolovanje virusa Newcastle bolesti iz suspektnog materijala kao i za njegovu identifikaciju, danas su sve više u upotrebi i molekularne metode dijagnostike i to pre svega lančana reakcija polimeraze (RT-PCR) i metoda sekvenciranja. Cilj ovog rada bio je ispitivanje mogućnosti primene navedenih metoda u dijagnostici infekcije živine izazvane virusom Newcastle bolesti. Prisustvo hemaglutinacionih antigena virusa Newcastle bolesti ustanovljeno je u uzorcima alantoisne tečnosti od 62 embrionirana jaja živine posle 72h od inokulacije čiji su se titri kretali od 1:16 do 1:2048, dok je testom heminhibicije (HI testom) uz primenu referentnog imunog seruma protiv navedenog uzročnika izvršena identifikacija izolovanih virusa u razređenjima seruma od 1:128 do 1:1024. Metodom reverzne transkripcije (RT-PCT) i lančane reakcije polimeraze (PCR) ustanovljeno da se kod osam ispitivanih uzoraka formira po jedan fragment virusne RNK u gelu agaroze veličine od 254bp koji je po sekvenci nukleotida karakterističan za genom virusa Newcastle bolesti. Na osnovu uporedne analize sekvenci RNK dobijenih od osam izolovanih sojeva virusa NDV i sekvenci genoma referentnih sojeva virusa atipične kuge živine uz korišćenje Mega 40 i BLAST programa, utvrđeno je da su izolovani sojevi virusa Newcastle bolesti visoko virulentni.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics, Identifikacija izolovanih sojeva virusa atipične kuge živine primenom molekularnih metoda virusološke dijagnostike",
volume = "62",
number = "3-4",
pages = "167-177",
doi = "10.2298/VETGL0804167V"
}

Vidanović, D., Šekler, M., Vasković, N., Žarković, A., Matović, K., Milić, N.,& Nišavić, J.. (2008). Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(3-4), 167-177.
https://doi.org/10.2298/VETGL0804167V

Vidanović D, Šekler M, Vasković N, Žarković A, Matović K, Milić N, Nišavić J. Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics. in Veterinarski Glasnik. 2008;62(3-4):167-177.
doi:10.2298/VETGL0804167V .

Vidanović, Dejan, Šekler, Milanko, Vasković, Nikola, Žarković, Aleksandar, Matović, Kazimir, Milić, Nenad, Nišavić, Jakov, "Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics" in Veterinarski Glasnik, 62, no. 3-4 (2008):167-177,
https://doi.org/10.2298/VETGL0804167V . .