TY  - JOUR
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja M.
AU  - Dominiković, Nina
AU  - Lakić, Nada
AU  - Ćosić, Milivoje
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3836
AB  - Nosema ceranae, a microsporidian parasite, as one of the stressors that contribute to honey bee decline, has a significant negative impact on the longevity, productivity, and reproductive capacity of honey bee colonies. There are several different strategies for Nosema infection control, including natural-based and antibiotic-based products. In this study, we tested wormwood and oak bark-based supplement “Medenko forte” on survival, Nosema infection, oxidative stress, and expression of immune-related genes in artificially N. ceranae-infected bees. The results revealed a positive influence on the survival of Nosema-infected bees, irrespectively of the moment of supplement application (day 1, day 3, or day 6 after bee emergence), as well as reduction of Nosema loads and, consequently, Nosema-induced oxidative stress. Supplementation had no negative effects on bee immunity, but better anti-Nosema than immune-stimulating effects were affirmed based on expression levels of abaecin, defensin, hymenoptaecin, apidaecin, and vitellogenin genes. In conclusion, the tested supplement „Medenko forte” has great potential in the health protection of Nosema-infected bees. However, further investigations need to be performed to elucidate its mechanisms of action.
PB  - MDPI
T2  - Animals
T1  - Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees
VL  - 14
IS  - 8
SP  - 1195
DO  - 10.3390/ani14081195
ER  - 

@article{
author = "Glavinić, Uroš and Jovanović, Nemanja M. and Dominiković, Nina and Lakić, Nada and Ćosić, Milivoje and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2024",
abstract = "Nosema ceranae, a microsporidian parasite, as one of the stressors that contribute to honey bee decline, has a significant negative impact on the longevity, productivity, and reproductive capacity of honey bee colonies. There are several different strategies for Nosema infection control, including natural-based and antibiotic-based products. In this study, we tested wormwood and oak bark-based supplement “Medenko forte” on survival, Nosema infection, oxidative stress, and expression of immune-related genes in artificially N. ceranae-infected bees. The results revealed a positive influence on the survival of Nosema-infected bees, irrespectively of the moment of supplement application (day 1, day 3, or day 6 after bee emergence), as well as reduction of Nosema loads and, consequently, Nosema-induced oxidative stress. Supplementation had no negative effects on bee immunity, but better anti-Nosema than immune-stimulating effects were affirmed based on expression levels of abaecin, defensin, hymenoptaecin, apidaecin, and vitellogenin genes. In conclusion, the tested supplement „Medenko forte” has great potential in the health protection of Nosema-infected bees. However, further investigations need to be performed to elucidate its mechanisms of action.",
publisher = "MDPI",
journal = "Animals",
title = "Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees",
volume = "14",
number = "8",
pages = "1195",
doi = "10.3390/ani14081195"
}

Glavinić, U., Jovanović, N. M., Dominiković, N., Lakić, N., Ćosić, M., Stevanović, J.,& Stanimirović, Z.. (2024). Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees. in Animals
MDPI., 14(8), 1195.
https://doi.org/10.3390/ani14081195

Glavinić U, Jovanović NM, Dominiković N, Lakić N, Ćosić M, Stevanović J, Stanimirović Z. Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees. in Animals. 2024;14(8):1195.
doi:10.3390/ani14081195 .

Glavinić, Uroš, Jovanović, Nemanja M., Dominiković, Nina, Lakić, Nada, Ćosić, Milivoje, Stevanović, Jevrosima, Stanimirović, Zoran, "Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees" in Animals, 14, no. 8 (2024):1195,
https://doi.org/10.3390/ani14081195 . .

TY  - JOUR
AU  - Marinković, Darko
AU  - Relić, Renata
AU  - Lakić, Nada
AU  - Aničić, Milan
AU  - Beuković, Dejan
AU  - Davidović, Vesna
AU  - Lavadinović, Vukan
AU  - Popović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3339
AB  - The lifespan of the European hare (Lepus europaeus P.) is affected by a number of negative
factors, including pollutants. In this paper, the individual and joint influence of age and habitat
(elevation and distance from the thermal power plant - TPP) on pathomorphological findings
of hares shot during three hunting seasons was investigated. Pathomorphological changes
were found in 95.12% of hares. In hares up to 1 year of age, the changes were predominant
in the lungs, and in older hares, in the kidneys. Degenerative changes in kidneys and liver and
inflammatory changes in kidneys and lungs were considered important most in discussing the
influence of chemical pollution. The proximity of TPP influenced the type of changes in the
liver. A significant joint effect of age and elevation on the type of changes in the lungs of
adult hares and on the heart of young hares was found. Elevation and distance from TPP had
a joint effect on the occurrence of changes in the lungs, intestines, and heart in hares from
the field farther from TPP. The results indicate that the hares were highly exposed to chemical
pollutants that may affect their immunity, and lifespan.
PB  - Taylor & Francis Group
T2  - Veterinary Quarterly
T1  - Influence of age, habitat elevation, and distance to a thermal power plant on pathomorphological findings in the European brown hare (Lepuseuropaeus P.)
VL  - 43
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1080/01652176.2023.2273887
ER  - 

@article{
author = "Marinković, Darko and Relić, Renata and Lakić, Nada and Aničić, Milan and Beuković, Dejan and Davidović, Vesna and Lavadinović, Vukan and Popović, Zoran",
year = "2023",
abstract = "The lifespan of the European hare (Lepus europaeus P.) is affected by a number of negative
factors, including pollutants. In this paper, the individual and joint influence of age and habitat
(elevation and distance from the thermal power plant - TPP) on pathomorphological findings
of hares shot during three hunting seasons was investigated. Pathomorphological changes
were found in 95.12% of hares. In hares up to 1 year of age, the changes were predominant
in the lungs, and in older hares, in the kidneys. Degenerative changes in kidneys and liver and
inflammatory changes in kidneys and lungs were considered important most in discussing the
influence of chemical pollution. The proximity of TPP influenced the type of changes in the
liver. A significant joint effect of age and elevation on the type of changes in the lungs of
adult hares and on the heart of young hares was found. Elevation and distance from TPP had
a joint effect on the occurrence of changes in the lungs, intestines, and heart in hares from
the field farther from TPP. The results indicate that the hares were highly exposed to chemical
pollutants that may affect their immunity, and lifespan.",
publisher = "Taylor & Francis Group",
journal = "Veterinary Quarterly",
title = "Influence of age, habitat elevation, and distance to a thermal power plant on pathomorphological findings in the European brown hare (Lepuseuropaeus P.)",
volume = "43",
number = "1",
pages = "1-10",
doi = "10.1080/01652176.2023.2273887"
}

Marinković, D., Relić, R., Lakić, N., Aničić, M., Beuković, D., Davidović, V., Lavadinović, V.,& Popović, Z.. (2023). Influence of age, habitat elevation, and distance to a thermal power plant on pathomorphological findings in the European brown hare (Lepuseuropaeus P.). in Veterinary Quarterly
Taylor & Francis Group., 43(1), 1-10.
https://doi.org/10.1080/01652176.2023.2273887

Marinković D, Relić R, Lakić N, Aničić M, Beuković D, Davidović V, Lavadinović V, Popović Z. Influence of age, habitat elevation, and distance to a thermal power plant on pathomorphological findings in the European brown hare (Lepuseuropaeus P.). in Veterinary Quarterly. 2023;43(1):1-10.
doi:10.1080/01652176.2023.2273887 .

Marinković, Darko, Relić, Renata, Lakić, Nada, Aničić, Milan, Beuković, Dejan, Davidović, Vesna, Lavadinović, Vukan, Popović, Zoran, "Influence of age, habitat elevation, and distance to a thermal power plant on pathomorphological findings in the European brown hare (Lepuseuropaeus P.)" in Veterinary Quarterly, 43, no. 1 (2023):1-10,
https://doi.org/10.1080/01652176.2023.2273887 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Blagojević, Jovan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Mirilović, Milorad
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2467
AB  - Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.
PB  - MDPI
T2  - Insects
T1  - Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees
VL  - 13
IS  - 7
SP  - 574
DO  - 10.3390/insects13070574
ER  - 

@article{
author = "Glavinić, Uroš and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Lakić, Nada and Mirilović, Milorad and Stanimirović, Zoran",
year = "2022",
abstract = "Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.",
publisher = "MDPI",
journal = "Insects",
title = "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees",
volume = "13",
number = "7",
pages = "574",
doi = "10.3390/insects13070574"
}

Glavinić, U., Blagojević, J., Ristanić, M., Stevanović, J., Lakić, N., Mirilović, M.,& Stanimirović, Z.. (2022). Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects
MDPI., 13(7), 574.
https://doi.org/10.3390/insects13070574

Glavinić U, Blagojević J, Ristanić M, Stevanović J, Lakić N, Mirilović M, Stanimirović Z. Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects. 2022;13(7):574.
doi:10.3390/insects13070574 .

Glavinić, Uroš, Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Lakić, Nada, Mirilović, Milorad, Stanimirović, Zoran, "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees" in Insects, 13, no. 7 (2022):574,
https://doi.org/10.3390/insects13070574 . .

TY  - DATA
AU  - Glavinić, Uroš
AU  - Blagojević, Jovan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Mirilović, Milorad
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2468
AB  - Figure S1. Expression levels of abaecin, hymenoptaecin, defensin, apidaecin and vitellogenin at different time points (day 6, 9 and 15) in experimental groups. N. ceranae infected control (I) and groups infected and supplemented with thymol from day 1 (I-T1), day 3 (I-T3) and day 6 (I-T6)
PB  - MDPI
T2  - Insects
T1  - Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.
VL  - 13
IS  - 7
SP  - 574
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2468
ER  - 

@misc{
author = "Glavinić, Uroš and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Lakić, Nada and Mirilović, Milorad and Stanimirović, Zoran",
year = "2022",
abstract = "Figure S1. Expression levels of abaecin, hymenoptaecin, defensin, apidaecin and vitellogenin at different time points (day 6, 9 and 15) in experimental groups. N. ceranae infected control (I) and groups infected and supplemented with thymol from day 1 (I-T1), day 3 (I-T3) and day 6 (I-T6)",
publisher = "MDPI",
journal = "Insects",
title = "Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.",
volume = "13",
number = "7",
pages = "574",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2468"
}

Glavinić, U., Blagojević, J., Ristanić, M., Stevanović, J., Lakić, N., Mirilović, M.,& Stanimirović, Z.. (2022). Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.. in Insects
MDPI., 13(7), 574.
https://hdl.handle.net/21.15107/rcub_veterinar_2468

Glavinić U, Blagojević J, Ristanić M, Stevanović J, Lakić N, Mirilović M, Stanimirović Z. Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.. in Insects. 2022;13(7):574.
https://hdl.handle.net/21.15107/rcub_veterinar_2468 .

Glavinić, Uroš, Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Lakić, Nada, Mirilović, Milorad, Stanimirović, Zoran, "Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574." in Insects, 13, no. 7 (2022):574,
https://hdl.handle.net/21.15107/rcub_veterinar_2468 .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Davitkov, Dajana
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2047
AB  - Depending on the infection level and colony strength, Nosema ceranae, a microsporidian endoparasite of the honey bee may have significant consequences on the health, reproduction and productivity of bee colonies. Despite exerting some side effects, fumagillin is most often used for Nosema control. In this study, in a cage experiment, N. ceranae infected bees were treated with fumagillin or the extract of Agaricus blazei mushroom, a possible alternative for Nosema control. Bee survival, Nosema spore loads, the expression levels of immune-related genes and parameters of oxidative stress were observed. Fumagillin treatment showed a negative effect on monitored parameters when applied preventively to non-infected bees, while a noticeable anti-Nosema effect and protection from Nosema-induced immunosuppression and oxidative stress were proven in Nosema-infected bees. However, a protective effect of the natural A. blazei extract was detected, without any side effects but with immunostimulatory activity in the preventive application. The results of this research suggest the potential of A. blazei extract for Nosema control, which needs to be further investigated.
PB  - MDPI
T2  - Insects
T1  - Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees
VL  - 12
IS  - 4
SP  - 282
DO  - 10.3390/insects12040282
ER  - 

@article{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Ristanić, Marko and Rajković, Milan and Davitkov, Dajana and Lakić, Nada and Stanimirović, Zoran",
year = "2021",
abstract = "Depending on the infection level and colony strength, Nosema ceranae, a microsporidian endoparasite of the honey bee may have significant consequences on the health, reproduction and productivity of bee colonies. Despite exerting some side effects, fumagillin is most often used for Nosema control. In this study, in a cage experiment, N. ceranae infected bees were treated with fumagillin or the extract of Agaricus blazei mushroom, a possible alternative for Nosema control. Bee survival, Nosema spore loads, the expression levels of immune-related genes and parameters of oxidative stress were observed. Fumagillin treatment showed a negative effect on monitored parameters when applied preventively to non-infected bees, while a noticeable anti-Nosema effect and protection from Nosema-induced immunosuppression and oxidative stress were proven in Nosema-infected bees. However, a protective effect of the natural A. blazei extract was detected, without any side effects but with immunostimulatory activity in the preventive application. The results of this research suggest the potential of A. blazei extract for Nosema control, which needs to be further investigated.",
publisher = "MDPI",
journal = "Insects",
title = "Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees",
volume = "12",
number = "4",
pages = "282",
doi = "10.3390/insects12040282"
}

Glavinić, U., Stevanović, J., Ristanić, M., Rajković, M., Davitkov, D., Lakić, N.,& Stanimirović, Z.. (2021). Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees. in Insects
MDPI., 12(4), 282.
https://doi.org/10.3390/insects12040282

Glavinić U, Stevanović J, Ristanić M, Rajković M, Davitkov D, Lakić N, Stanimirović Z. Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees. in Insects. 2021;12(4):282.
doi:10.3390/insects12040282 .

Glavinić, Uroš, Stevanović, Jevrosima, Ristanić, Marko, Rajković, Milan, Davitkov, Dajana, Lakić, Nada, Stanimirović, Zoran, "Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees" in Insects, 12, no. 4 (2021):282,
https://doi.org/10.3390/insects12040282 . .

TY  - DATA
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Davitkov, Dajana
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2048
PB  - MDPI
T2  - Insects
T1  - Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2048
ER  - 

@misc{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Ristanić, Marko and Rajković, Milan and Davitkov, Dajana and Lakić, Nada and Stanimirović, Zoran",
year = "2021",
publisher = "MDPI",
journal = "Insects",
title = "Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2048"
}

Glavinić, U., Stevanović, J., Ristanić, M., Rajković, M., Davitkov, D., Lakić, N.,& Stanimirović, Z.. (2021). Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.. in Insects
MDPI..
https://hdl.handle.net/21.15107/rcub_veterinar_2048

Glavinić U, Stevanović J, Ristanić M, Rajković M, Davitkov D, Lakić N, Stanimirović Z. Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.. in Insects. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_2048 .

Glavinić, Uroš, Stevanović, Jevrosima, Ristanić, Marko, Rajković, Milan, Davitkov, Dajana, Lakić, Nada, Stanimirović, Zoran, "Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282." in Insects (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_2048 .

TY  - JOUR
AU  - Relić, Renata
AU  - Lakić, Nada
AU  - Janković, Ljiljana
AU  - Davidović, Vesna
AU  - Staric, Joze
AU  - Jezek, Jozica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2053
AB  - Aim of the study: Calf rearing practices vary in different countries and may be affected by many factors. Poor management is related to diseases outbreak and death in calves. This study aimed to analyze practices in calf rearing and the occurrence of common calf diseases on family farms in two European countries and to examine the characteristics of a farmer and his farm as factors that may affect the way of performing practices related to calves’ health.Area of study: Slovenia and Serbia.Material and methods: For collecting data, the same questionnaire-based survey was distributed among cattle farm owners in Slovenia and Serbia.Main results: The following factors showed a significant influence (p<0.05) on certain rearing practices: farm size (on time for checking calves, milk reheating, and providing rehydration fluids to the calf with diarrhea in Slovenia), specialization of the production (on preventive antiparasitic treatments and restriction of drinking water for calves with diarrhea in Slovenia, and the time for checking calves in Serbia), breeder’s age (on colostrum quality checking in Slovenia), and breeder’s education (on time for checking calves and checking colostrum quality in Serbia). The results pointed out the rearing practices to be improved in both countries, Slovenia and Serbia, such as colostrum management and prevention strategies of calf diseases.Research highlights: Characteristics of the farmer and his farm may affect many aspects of calves’ rearing. Continuous education of farmers and appropriate production planning can contribute to better farm productivity and better health and welfare of calves.
PB  - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria
T2  - Spanish Journal of Agricultural Research
T1  - Factors affecting rearing practices and health of calves on family farms
VL  - 19
IS  - 1
SP  - 0501
DO  - 10.5424/sjar/2021191-17181
ER  - 

@article{
author = "Relić, Renata and Lakić, Nada and Janković, Ljiljana and Davidović, Vesna and Staric, Joze and Jezek, Jozica",
year = "2021",
abstract = "Aim of the study: Calf rearing practices vary in different countries and may be affected by many factors. Poor management is related to diseases outbreak and death in calves. This study aimed to analyze practices in calf rearing and the occurrence of common calf diseases on family farms in two European countries and to examine the characteristics of a farmer and his farm as factors that may affect the way of performing practices related to calves’ health.Area of study: Slovenia and Serbia.Material and methods: For collecting data, the same questionnaire-based survey was distributed among cattle farm owners in Slovenia and Serbia.Main results: The following factors showed a significant influence (p<0.05) on certain rearing practices: farm size (on time for checking calves, milk reheating, and providing rehydration fluids to the calf with diarrhea in Slovenia), specialization of the production (on preventive antiparasitic treatments and restriction of drinking water for calves with diarrhea in Slovenia, and the time for checking calves in Serbia), breeder’s age (on colostrum quality checking in Slovenia), and breeder’s education (on time for checking calves and checking colostrum quality in Serbia). The results pointed out the rearing practices to be improved in both countries, Slovenia and Serbia, such as colostrum management and prevention strategies of calf diseases.Research highlights: Characteristics of the farmer and his farm may affect many aspects of calves’ rearing. Continuous education of farmers and appropriate production planning can contribute to better farm productivity and better health and welfare of calves.",
publisher = "Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria",
journal = "Spanish Journal of Agricultural Research",
title = "Factors affecting rearing practices and health of calves on family farms",
volume = "19",
number = "1",
pages = "0501",
doi = "10.5424/sjar/2021191-17181"
}

Relić, R., Lakić, N., Janković, L., Davidović, V., Staric, J.,& Jezek, J.. (2021). Factors affecting rearing practices and health of calves on family farms. in Spanish Journal of Agricultural Research
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria., 19(1), 0501.
https://doi.org/10.5424/sjar/2021191-17181

Relić R, Lakić N, Janković L, Davidović V, Staric J, Jezek J. Factors affecting rearing practices and health of calves on family farms. in Spanish Journal of Agricultural Research. 2021;19(1):0501.
doi:10.5424/sjar/2021191-17181 .

Relić, Renata, Lakić, Nada, Janković, Ljiljana, Davidović, Vesna, Staric, Joze, Jezek, Jozica, "Factors affecting rearing practices and health of calves on family farms" in Spanish Journal of Agricultural Research, 19, no. 1 (2021):0501,
https://doi.org/10.5424/sjar/2021191-17181 . .

TY  - JOUR
AU  - Petrović, Slobodan
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Maletić, Jelena
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2036
AB  - The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals.
AB  - Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions
T1  - Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa
VL  - 70
IS  - 4
SP  - 453
EP  - 470
DO  - 10.2478/acve-2020-0034
ER  - 

@article{
author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran",
year = "2020",
abstract = "The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals., Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions, Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa",
volume = "70",
number = "4",
pages = "453-470",
doi = "10.2478/acve-2020-0034"
}

Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2020). The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(4), 453-470.
https://doi.org/10.2478/acve-2020-0034

Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd. 2020;70(4):453-470.
doi:10.2478/acve-2020-0034 .

Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions" in Acta Veterinaria-Beograd, 70, no. 4 (2020):453-470,
https://doi.org/10.2478/acve-2020-0034 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Munoz, Irene
AU  - de la Rua, Pilar
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Kulišić, Zoran
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1787
AB  - The aim of this study was to investigate the genetic diversity of Varroa destructor parasitizing Apis mellifera colonies and to test for possible host-parasite association at the mitochondrial DNA (mtDNA) level. Six A. mellifera haplotypes (including a novel C2aa) and five haplotypes of V. destructor were detected in 29 analyzed colonies from eight sampling sites in Serbia. We revealed the presence of the K and S1 haplotypes as well as KS1 and KP1 heteroplasmic mite individuals in all localities, while the P1 haplotype was only found in four sampling sites. Significant differences in V. destructor genetic diversity were found at both apiary and colony levels, with mite haplotypes coexisting in almost all tested colonies. In addition, a significant correlation between the number of analyzed mites per colony and the number of identified V. destructor haplotypes was observed. However, no significant host-parasite relationship was found, suggesting that mites bearing different haplotypes as well as those heteroplasmic individuals are well adapted to the host, A. mellifera, independently of the identified haplotype present in each colony. Our results will contribute to future population and biogeographic studies concerning V. destructor infesting A. mellifera, as well as to better understanding their host-parasite relationship.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Coexistence of genetically different Varroa destructor in Apis mellifera colonies
VL  - 78
IS  - 3
SP  - 315
EP  - 326
DO  - 10.1007/s10493-019-00395-z
ER  - 

@article{
author = "Gajić, Bojan and Munoz, Irene and de la Rua, Pilar and Stevanović, Jevrosima and Lakić, Nada and Kulišić, Zoran and Stanimirović, Zoran",
year = "2019",
abstract = "The aim of this study was to investigate the genetic diversity of Varroa destructor parasitizing Apis mellifera colonies and to test for possible host-parasite association at the mitochondrial DNA (mtDNA) level. Six A. mellifera haplotypes (including a novel C2aa) and five haplotypes of V. destructor were detected in 29 analyzed colonies from eight sampling sites in Serbia. We revealed the presence of the K and S1 haplotypes as well as KS1 and KP1 heteroplasmic mite individuals in all localities, while the P1 haplotype was only found in four sampling sites. Significant differences in V. destructor genetic diversity were found at both apiary and colony levels, with mite haplotypes coexisting in almost all tested colonies. In addition, a significant correlation between the number of analyzed mites per colony and the number of identified V. destructor haplotypes was observed. However, no significant host-parasite relationship was found, suggesting that mites bearing different haplotypes as well as those heteroplasmic individuals are well adapted to the host, A. mellifera, independently of the identified haplotype present in each colony. Our results will contribute to future population and biogeographic studies concerning V. destructor infesting A. mellifera, as well as to better understanding their host-parasite relationship.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Coexistence of genetically different Varroa destructor in Apis mellifera colonies",
volume = "78",
number = "3",
pages = "315-326",
doi = "10.1007/s10493-019-00395-z"
}

Gajić, B., Munoz, I., de la Rua, P., Stevanović, J., Lakić, N., Kulišić, Z.,& Stanimirović, Z.. (2019). Coexistence of genetically different Varroa destructor in Apis mellifera colonies. in Experimental and Applied Acarology
Springer, Dordrecht., 78(3), 315-326.
https://doi.org/10.1007/s10493-019-00395-z

Gajić B, Munoz I, de la Rua P, Stevanović J, Lakić N, Kulišić Z, Stanimirović Z. Coexistence of genetically different Varroa destructor in Apis mellifera colonies. in Experimental and Applied Acarology. 2019;78(3):315-326.
doi:10.1007/s10493-019-00395-z .

Gajić, Bojan, Munoz, Irene, de la Rua, Pilar, Stevanović, Jevrosima, Lakić, Nada, Kulišić, Zoran, Stanimirović, Zoran, "Coexistence of genetically different Varroa destructor in Apis mellifera colonies" in Experimental and Applied Acarology, 78, no. 3 (2019):315-326,
https://doi.org/10.1007/s10493-019-00395-z . .

TY  - JOUR
AU  - Delić, Nikola
AU  - Drašković, Vladimir
AU  - Stevanović, Jevrosima
AU  - Savić, Božidar
AU  - Lakić, Nada
AU  - Bošnjak-Neumuller, Jasna
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1656
AB  - Control of swine dysentery with antibiotics is often ineffective due to the resistance of Brachyspira hyodysenteriae. The potential of some herbal-based components against B. hyodysenteriae was previously studied in vitro. This study aims at the evaluation of in vivo efficacy of phytogemc feed additives in the control of swine dysentery. The study involved 64 seven-week old weaned pigs allotted to 4 groups: two were fed on feed supplemented with either Patente Herba(R)or Patente Herba(R) Plus, the third received tiamulin (positive control), while the negative control was not given antibiotics or additives. Fecal consistency was recorded daily. The presence of B. hyodysenteriae in the feces was investigated weekly using microbiological assays and the PCR test. Weight gain and feed conversion ratio were calculated for each week, and for the whole experiment. B. hyodysenteriae was detected in all samples by both methods. The additives showed efficacy in the prevention and control of swine dysentery as only normal and soft stool was observed in the treated groups. By contrast, in the negative control all feces categories were detected. Frequencies of feces categories significantly differed (p<0.001) between feed-supplemented groups and the negative control. Efficacy of both additives in the prevention of SD is comparable to tiamulin, based on insignificant differences in the frequency of the various feces categories. Beneficial effects of both additives resulted in significantly (p <= 0.05) higher weight gain and lower feed conversion ratio in comparison to the negative control. The average weight gains between additive-fed groups and tiamulin-treated group did not differ significantly.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The efficacy of two phytogenic feed additives in the control of swine dysentery
VL  - 68
IS  - 2
SP  - 178
EP  - 189
DO  - 10.2478/acve-2018-0016
ER  - 

@article{
author = "Delić, Nikola and Drašković, Vladimir and Stevanović, Jevrosima and Savić, Božidar and Lakić, Nada and Bošnjak-Neumuller, Jasna and Stanimirović, Zoran",
year = "2018",
abstract = "Control of swine dysentery with antibiotics is often ineffective due to the resistance of Brachyspira hyodysenteriae. The potential of some herbal-based components against B. hyodysenteriae was previously studied in vitro. This study aims at the evaluation of in vivo efficacy of phytogemc feed additives in the control of swine dysentery. The study involved 64 seven-week old weaned pigs allotted to 4 groups: two were fed on feed supplemented with either Patente Herba(R)or Patente Herba(R) Plus, the third received tiamulin (positive control), while the negative control was not given antibiotics or additives. Fecal consistency was recorded daily. The presence of B. hyodysenteriae in the feces was investigated weekly using microbiological assays and the PCR test. Weight gain and feed conversion ratio were calculated for each week, and for the whole experiment. B. hyodysenteriae was detected in all samples by both methods. The additives showed efficacy in the prevention and control of swine dysentery as only normal and soft stool was observed in the treated groups. By contrast, in the negative control all feces categories were detected. Frequencies of feces categories significantly differed (p<0.001) between feed-supplemented groups and the negative control. Efficacy of both additives in the prevention of SD is comparable to tiamulin, based on insignificant differences in the frequency of the various feces categories. Beneficial effects of both additives resulted in significantly (p <= 0.05) higher weight gain and lower feed conversion ratio in comparison to the negative control. The average weight gains between additive-fed groups and tiamulin-treated group did not differ significantly.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The efficacy of two phytogenic feed additives in the control of swine dysentery",
volume = "68",
number = "2",
pages = "178-189",
doi = "10.2478/acve-2018-0016"
}

Delić, N., Drašković, V., Stevanović, J., Savić, B., Lakić, N., Bošnjak-Neumuller, J.,& Stanimirović, Z.. (2018). The efficacy of two phytogenic feed additives in the control of swine dysentery. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(2), 178-189.
https://doi.org/10.2478/acve-2018-0016

Delić N, Drašković V, Stevanović J, Savić B, Lakić N, Bošnjak-Neumuller J, Stanimirović Z. The efficacy of two phytogenic feed additives in the control of swine dysentery. in Acta Veterinaria-Beograd. 2018;68(2):178-189.
doi:10.2478/acve-2018-0016 .

Delić, Nikola, Drašković, Vladimir, Stevanović, Jevrosima, Savić, Božidar, Lakić, Nada, Bošnjak-Neumuller, Jasna, Stanimirović, Zoran, "The efficacy of two phytogenic feed additives in the control of swine dysentery" in Acta Veterinaria-Beograd, 68, no. 2 (2018):178-189,
https://doi.org/10.2478/acve-2018-0016 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Simeunović, Predrag
AU  - Lakić, Nada
AU  - Radović, Ivica
AU  - Soković, Marina
AU  - Van Griensven, Leo J. L. D.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1630
AB  - This study was done to discover any beneficial effect of a medicinal mushroom Agaricus brasiliensis extract on the honey bee. Firstly, a laboratory experiment was conducted on 640 bees reared in 32 single-use plastic rearing cups. A. brasiliensis extract proved safe in all doses tested (50, 100 and 150 mg/kg/day) irrespective of feeding mode (sugar syrup or candy). Secondly, a three-year field experiment was conducted on 26 colonies treated with a single dose of A. brasiliensis extract (100 mg/kg/day) added to syrup. Each year the colonies were treated once in autumn and twice in spring. The treatments significantly increased colony strength parameters: brood rearing improvement and adult population growth were noticed more often than the increase in honey production and pollen reserves. These positive effects were mainly observed in April. In conclusion, A. brasiliensis extract is safe for the bees and helps maintaining strong colonies, especially in spring.
PB  - Acad Brasileira De Ciencias, Rio Janeiro
T2  - Anais da Academia Brasileira de Ciencias
T1  - The effect of Agaricus brasiliensis extract supplementation on honey bee colonies
VL  - 90
IS  - 1
SP  - 219
EP  - 229
DO  - 10.1590/0001-3765201820150182
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Simeunović, Predrag and Lakić, Nada and Radović, Ivica and Soković, Marina and Van Griensven, Leo J. L. D.",
year = "2018",
abstract = "This study was done to discover any beneficial effect of a medicinal mushroom Agaricus brasiliensis extract on the honey bee. Firstly, a laboratory experiment was conducted on 640 bees reared in 32 single-use plastic rearing cups. A. brasiliensis extract proved safe in all doses tested (50, 100 and 150 mg/kg/day) irrespective of feeding mode (sugar syrup or candy). Secondly, a three-year field experiment was conducted on 26 colonies treated with a single dose of A. brasiliensis extract (100 mg/kg/day) added to syrup. Each year the colonies were treated once in autumn and twice in spring. The treatments significantly increased colony strength parameters: brood rearing improvement and adult population growth were noticed more often than the increase in honey production and pollen reserves. These positive effects were mainly observed in April. In conclusion, A. brasiliensis extract is safe for the bees and helps maintaining strong colonies, especially in spring.",
publisher = "Acad Brasileira De Ciencias, Rio Janeiro",
journal = "Anais da Academia Brasileira de Ciencias",
title = "The effect of Agaricus brasiliensis extract supplementation on honey bee colonies",
volume = "90",
number = "1",
pages = "219-229",
doi = "10.1590/0001-3765201820150182"
}

Stevanović, J., Stanimirović, Z., Simeunović, P., Lakić, N., Radović, I., Soković, M.,& Van Griensven, L. J. L. D.. (2018). The effect of Agaricus brasiliensis extract supplementation on honey bee colonies. in Anais da Academia Brasileira de Ciencias
Acad Brasileira De Ciencias, Rio Janeiro., 90(1), 219-229.
https://doi.org/10.1590/0001-3765201820150182

Stevanović J, Stanimirović Z, Simeunović P, Lakić N, Radović I, Soković M, Van Griensven LJLD. The effect of Agaricus brasiliensis extract supplementation on honey bee colonies. in Anais da Academia Brasileira de Ciencias. 2018;90(1):219-229.
doi:10.1590/0001-3765201820150182 .

Stevanović, Jevrosima, Stanimirović, Zoran, Simeunović, Predrag, Lakić, Nada, Radović, Ivica, Soković, Marina, Van Griensven, Leo J. L. D., "The effect of Agaricus brasiliensis extract supplementation on honey bee colonies" in Anais da Academia Brasileira de Ciencias, 90, no. 1 (2018):219-229,
https://doi.org/10.1590/0001-3765201820150182 . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Glavinić, Uroš
AU  - Lakić, Nada
AU  - Radović, Dejan
AU  - Ristanić, Marko
AU  - Tarić, Elmin
AU  - Stevanović, Jevrosima
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1464
AB  - Varroa destructor is the most important honey bee parasite. There are various methods used in the control of this mite, but none of them meets all requested criteria, to be safe, effective and easy to apply. The objective of this study was to evaluate the varroacidal efficacy of newly created plant-derived formulation Argus Ras (mixture of extracts of Sophora flavescens, Ginkgo biloba, Gleditsia chinensis and Teucrium chamaedrys) in a field trial. The investigation was conducted on 240 Apis mellifera colonies equalized in respect of brood amount, adult bee population and food reserves. Efficiency was evaluated by applying Argus Ras consecutively with two other acaricides, amitraz and oxalic acid. Average acaricidal efficacy of Argus Ras was 80.89%, being higher of other previously tested essential oils. Besides, it showed a potential in knocking down the mites resistant to other acaricides. It should not be neglected that Argus Ras requires a smaller number of treatments and financial investments than other formulations used for the control of Varroa mites.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Efficacy of plant-derived formulation argus ras in varroa destructor control
VL  - 67
IS  - 2
SP  - 191
EP  - 200
DO  - 10.1515/acve-2017-0017
ER  - 

@article{
author = "Stanimirović, Zoran and Glavinić, Uroš and Lakić, Nada and Radović, Dejan and Ristanić, Marko and Tarić, Elmin and Stevanović, Jevrosima",
year = "2017",
abstract = "Varroa destructor is the most important honey bee parasite. There are various methods used in the control of this mite, but none of them meets all requested criteria, to be safe, effective and easy to apply. The objective of this study was to evaluate the varroacidal efficacy of newly created plant-derived formulation Argus Ras (mixture of extracts of Sophora flavescens, Ginkgo biloba, Gleditsia chinensis and Teucrium chamaedrys) in a field trial. The investigation was conducted on 240 Apis mellifera colonies equalized in respect of brood amount, adult bee population and food reserves. Efficiency was evaluated by applying Argus Ras consecutively with two other acaricides, amitraz and oxalic acid. Average acaricidal efficacy of Argus Ras was 80.89%, being higher of other previously tested essential oils. Besides, it showed a potential in knocking down the mites resistant to other acaricides. It should not be neglected that Argus Ras requires a smaller number of treatments and financial investments than other formulations used for the control of Varroa mites.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Efficacy of plant-derived formulation argus ras in varroa destructor control",
volume = "67",
number = "2",
pages = "191-200",
doi = "10.1515/acve-2017-0017"
}

Stanimirović, Z., Glavinić, U., Lakić, N., Radović, D., Ristanić, M., Tarić, E.,& Stevanović, J.. (2017). Efficacy of plant-derived formulation argus ras in varroa destructor control. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 191-200.
https://doi.org/10.1515/acve-2017-0017

Stanimirović Z, Glavinić U, Lakić N, Radović D, Ristanić M, Tarić E, Stevanović J. Efficacy of plant-derived formulation argus ras in varroa destructor control. in Acta Veterinaria-Beograd. 2017;67(2):191-200.
doi:10.1515/acve-2017-0017 .

Stanimirović, Zoran, Glavinić, Uroš, Lakić, Nada, Radović, Dejan, Ristanić, Marko, Tarić, Elmin, Stevanović, Jevrosima, "Efficacy of plant-derived formulation argus ras in varroa destructor control" in Acta Veterinaria-Beograd, 67, no. 2 (2017):191-200,
https://doi.org/10.1515/acve-2017-0017 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stanković, Biljana
AU  - Drašković, Vladimir
AU  - Stevanović, Jevrosima
AU  - Petrović, Tamaš
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1541
AB  - Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex BEEWELL AminoPlus to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae
VL  - 12
IS  - 11
SP  - e0187726
DO  - 10.1371/journal.pone.0187726
ER  - 

@article{
author = "Glavinić, Uroš and Stanković, Biljana and Drašković, Vladimir and Stevanović, Jevrosima and Petrović, Tamaš and Lakić, Nada and Stanimirović, Zoran",
year = "2017",
abstract = "Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex BEEWELL AminoPlus to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae",
volume = "12",
number = "11",
pages = "e0187726",
doi = "10.1371/journal.pone.0187726"
}

Glavinić, U., Stanković, B., Drašković, V., Stevanović, J., Petrović, T., Lakić, N.,& Stanimirović, Z.. (2017). Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One
Public Library Science, San Francisco., 12(11), e0187726.
https://doi.org/10.1371/journal.pone.0187726

Glavinić U, Stanković B, Drašković V, Stevanović J, Petrović T, Lakić N, Stanimirović Z. Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One. 2017;12(11):e0187726.
doi:10.1371/journal.pone.0187726 .

Glavinić, Uroš, Stanković, Biljana, Drašković, Vladimir, Stevanović, Jevrosima, Petrović, Tamaš, Lakić, Nada, Stanimirović, Zoran, "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae" in PLoS One, 12, no. 11 (2017):e0187726,
https://doi.org/10.1371/journal.pone.0187726 . .

TY  - JOUR
AU  - Dacić, Stefan
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Lakić, Nada
AU  - Veselinović, Aleksandar
AU  - Ristanić, Marko
AU  - Anđelković, Marko
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1408
AB  - Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay
VL  - 48
IS  - 2
SP  - 617
EP  - 627
DO  - 10.2298/GENSR1602617D
ER  - 

@article{
author = "Dacić, Stefan and Đelić, Ninoslav and Radaković, Milena and Lakić, Nada and Veselinović, Aleksandar and Ristanić, Marko and Anđelković, Marko",
year = "2016",
abstract = "Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay",
volume = "48",
number = "2",
pages = "617-627",
doi = "10.2298/GENSR1602617D"
}

Dacić, S., Đelić, N., Radaković, M., Lakić, N., Veselinović, A., Ristanić, M.,& Anđelković, M.. (2016). Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika
Društvo genetičara Srbije, Beograd., 48(2), 617-627.
https://doi.org/10.2298/GENSR1602617D

Dacić S, Đelić N, Radaković M, Lakić N, Veselinović A, Ristanić M, Anđelković M. Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika. 2016;48(2):617-627.
doi:10.2298/GENSR1602617D .

Dacić, Stefan, Đelić, Ninoslav, Radaković, Milena, Lakić, Nada, Veselinović, Aleksandar, Ristanić, Marko, Anđelković, Marko, "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay" in Genetika, 48, no. 2 (2016):617-627,
https://doi.org/10.2298/GENSR1602617D . .

TY  - JOUR
AU  - Maletić, Jelena
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Kukolj, Vladimir
AU  - Aleksić, Nevenka
AU  - Anđelković, Marko
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1183
AB  - Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
VL  - 47
IS  - 1
SP  - 339
EP  - 348
DO  - 10.2298/GENSR1501339M
ER  - 

@article{
author = "Maletić, Jelena and Đelić, Ninoslav and Radaković, Milena and Maletić, Milan and Lakić, Nada and Kukolj, Vladimir and Aleksić, Nevenka and Anđelković, Marko and Stanimirović, Zoran",
year = "2015",
abstract = "Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro",
volume = "47",
number = "1",
pages = "339-348",
doi = "10.2298/GENSR1501339M"
}

Maletić, J., Đelić, N., Radaković, M., Maletić, M., Lakić, N., Kukolj, V., Aleksić, N., Anđelković, M.,& Stanimirović, Z.. (2015). Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 339-348.
https://doi.org/10.2298/GENSR1501339M

Maletić J, Đelić N, Radaković M, Maletić M, Lakić N, Kukolj V, Aleksić N, Anđelković M, Stanimirović Z. Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika. 2015;47(1):339-348.
doi:10.2298/GENSR1501339M .

Maletić, Jelena, Đelić, Ninoslav, Radaković, Milena, Maletić, Milan, Lakić, Nada, Kukolj, Vladimir, Aleksić, Nevenka, Anđelković, Marko, Stanimirović, Zoran, "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro" in Genetika, 47, no. 1 (2015):339-348,
https://doi.org/10.2298/GENSR1501339M . .

TY  - JOUR
AU  - Stanišić, Ljubodrag
AU  - Dimitrijević, Vladimir
AU  - Simeunović, Predrag
AU  - Lakić, Nada
AU  - Radović, Ivica
AU  - Ivanković, Ante
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1174
AB  - The aim of the study was to establish morphometric, biochemical and hematological values for the endangered Balkan donkey breed (Serbia) and to explore the possible age dependence of the parameters tested. Inter-breed similarity of morphometric parameters was assessed by comparing the data obtained for the Balkan donkey with morphometric measurements of several previously characterized domestic donkey breeds. The study population included 74 donkeys, divided in two age groups (group A <= 3 years; group B > 3 years). In total, 18 morphometric, 13 hematological and 14 biochemical parameters were assessed. Significant morphometric differences (p < 0.05) in body length, head length, chest circumference and body weight were found between the two age groups. Significant differences in morphological parameters were revealed among the Balkan donkey and other donkey breeds (Catalonian, Croatian and Albanian), but results of cluster analysis demonstrated the smallest distance between the Balkan donkey and Albanian donkeys. The results of morphometric analyses showed consistency of the obtained values within the breed, and diversity as compared to other donkey breeds, and, thus, could be taken as referent for the Balkan donkey. Hematological and biochemical profiles obtained for the Balkan donkey were consistent with previous reports and within the recommended reference ranges. White blood cell, mid cell and granulocyte counts, showed significantly higher (p < 0.05) values in donkeys under 3 years of age, while the only biochemical parameter affected by age was alkaline phosphatase. The information gained through characterization of the Balkan donkey breed provides a basis for conservation and development of the breed standard.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological, biochemical and hematological characterization of endangered balkan donkey breed
VL  - 65
IS  - 1
SP  - 125
EP  - 136
DO  - 10.1515/acve-2015-0010
ER  - 

@article{
author = "Stanišić, Ljubodrag and Dimitrijević, Vladimir and Simeunović, Predrag and Lakić, Nada and Radović, Ivica and Ivanković, Ante and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "The aim of the study was to establish morphometric, biochemical and hematological values for the endangered Balkan donkey breed (Serbia) and to explore the possible age dependence of the parameters tested. Inter-breed similarity of morphometric parameters was assessed by comparing the data obtained for the Balkan donkey with morphometric measurements of several previously characterized domestic donkey breeds. The study population included 74 donkeys, divided in two age groups (group A <= 3 years; group B > 3 years). In total, 18 morphometric, 13 hematological and 14 biochemical parameters were assessed. Significant morphometric differences (p < 0.05) in body length, head length, chest circumference and body weight were found between the two age groups. Significant differences in morphological parameters were revealed among the Balkan donkey and other donkey breeds (Catalonian, Croatian and Albanian), but results of cluster analysis demonstrated the smallest distance between the Balkan donkey and Albanian donkeys. The results of morphometric analyses showed consistency of the obtained values within the breed, and diversity as compared to other donkey breeds, and, thus, could be taken as referent for the Balkan donkey. Hematological and biochemical profiles obtained for the Balkan donkey were consistent with previous reports and within the recommended reference ranges. White blood cell, mid cell and granulocyte counts, showed significantly higher (p < 0.05) values in donkeys under 3 years of age, while the only biochemical parameter affected by age was alkaline phosphatase. The information gained through characterization of the Balkan donkey breed provides a basis for conservation and development of the breed standard.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological, biochemical and hematological characterization of endangered balkan donkey breed",
volume = "65",
number = "1",
pages = "125-136",
doi = "10.1515/acve-2015-0010"
}

Stanišić, L., Dimitrijević, V., Simeunović, P., Lakić, N., Radović, I., Ivanković, A., Stevanović, J.,& Stanimirović, Z.. (2015). Morphological, biochemical and hematological characterization of endangered balkan donkey breed. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 125-136.
https://doi.org/10.1515/acve-2015-0010

Stanišić L, Dimitrijević V, Simeunović P, Lakić N, Radović I, Ivanković A, Stevanović J, Stanimirović Z. Morphological, biochemical and hematological characterization of endangered balkan donkey breed. in Acta Veterinaria-Beograd. 2015;65(1):125-136.
doi:10.1515/acve-2015-0010 .

Stanišić, Ljubodrag, Dimitrijević, Vladimir, Simeunović, Predrag, Lakić, Nada, Radović, Ivica, Ivanković, Ante, Stevanović, Jevrosima, Stanimirović, Zoran, "Morphological, biochemical and hematological characterization of endangered balkan donkey breed" in Acta Veterinaria-Beograd, 65, no. 1 (2015):125-136,
https://doi.org/10.1515/acve-2015-0010 . .

TY  - JOUR
AU  - Simeunović, Predrag
AU  - Stevanović, Jevrosima
AU  - Ćirković, Dragan
AU  - Radojičić, Sonja
AU  - Lakić, Nada
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1096
AB  - The strength of the honey bee colony and the quality concerning brood production, number of worker bees and honey yield depend substantially upon the characteristics of the queen. Reproductive and productive capacities of the colony, as two important physiological qualities can depend a great deal on the age of the queen. Over the years, the laying ratio of queens could decrease depending on the age; therefore colonies headed by one-year-old queens have proven to be more efficient considering colony population and honey production than ones with older queens. Nosema ceranae infection is a possible reason for the decrease in reproductive capacities and the reduction in the honey production of infected colonies. With the help of numerous pheromones, the queen can preserve colony homeostasis, however exhausted by N. ceranae infection she might compromise the renewal of worker population. The aim of this research is to investigate the influence of N. c-eranae on reproduction and productivity of honey bee colonies headed by queens of different age. Experiment was conducted on 27 honey bee colonies equalized with regard to brood, food source, number of worker bees and Nosema infection. In the three year investigation period (2009-2012), Nosema detection, identification and spore load per each colony, as well as parameters of colony reproduction (such as uncapped and capped brood area, queen egg-laying rate and colony strength) and productivity (the total extracted honey and the winter honey stores) were recorded. Concerning the influence of the age of the queens on followed parameters we compared one, two and three-years old queens and found significantly higher reproductive and productive values in favour of younger ones. Significant differences regarding the effects of N. ceranae infection on productive and reproductive characteristics could be explained with a higher capacity of one year old queens to compensate the effects of Nosema infection; a capacity which gradually declines in the second and the third year.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony
VL  - 53
IS  - 5
SP  - 545
EP  - 554
DO  - 10.3896/IBRA.1.53.5.09
ER  - 

@article{
author = "Simeunović, Predrag and Stevanović, Jevrosima and Ćirković, Dragan and Radojičić, Sonja and Lakić, Nada and Stanišić, Ljubodrag and Stanimirović, Zoran",
year = "2014",
abstract = "The strength of the honey bee colony and the quality concerning brood production, number of worker bees and honey yield depend substantially upon the characteristics of the queen. Reproductive and productive capacities of the colony, as two important physiological qualities can depend a great deal on the age of the queen. Over the years, the laying ratio of queens could decrease depending on the age; therefore colonies headed by one-year-old queens have proven to be more efficient considering colony population and honey production than ones with older queens. Nosema ceranae infection is a possible reason for the decrease in reproductive capacities and the reduction in the honey production of infected colonies. With the help of numerous pheromones, the queen can preserve colony homeostasis, however exhausted by N. ceranae infection she might compromise the renewal of worker population. The aim of this research is to investigate the influence of N. c-eranae on reproduction and productivity of honey bee colonies headed by queens of different age. Experiment was conducted on 27 honey bee colonies equalized with regard to brood, food source, number of worker bees and Nosema infection. In the three year investigation period (2009-2012), Nosema detection, identification and spore load per each colony, as well as parameters of colony reproduction (such as uncapped and capped brood area, queen egg-laying rate and colony strength) and productivity (the total extracted honey and the winter honey stores) were recorded. Concerning the influence of the age of the queens on followed parameters we compared one, two and three-years old queens and found significantly higher reproductive and productive values in favour of younger ones. Significant differences regarding the effects of N. ceranae infection on productive and reproductive characteristics could be explained with a higher capacity of one year old queens to compensate the effects of Nosema infection; a capacity which gradually declines in the second and the third year.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony",
volume = "53",
number = "5",
pages = "545-554",
doi = "10.3896/IBRA.1.53.5.09"
}

Simeunović, P., Stevanović, J., Ćirković, D., Radojičić, S., Lakić, N., Stanišić, L.,& Stanimirović, Z.. (2014). Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 53(5), 545-554.
https://doi.org/10.3896/IBRA.1.53.5.09

Simeunović P, Stevanović J, Ćirković D, Radojičić S, Lakić N, Stanišić L, Stanimirović Z. Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. in Journal of Apicultural Research. 2014;53(5):545-554.
doi:10.3896/IBRA.1.53.5.09 .

Simeunović, Predrag, Stevanović, Jevrosima, Ćirković, Dragan, Radojičić, Sonja, Lakić, Nada, Stanišić, Ljubodrag, Stanimirović, Zoran, "Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony" in Journal of Apicultural Research, 53, no. 5 (2014):545-554,
https://doi.org/10.3896/IBRA.1.53.5.09 . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Kirovski, Danijela
AU  - Lakić, Nada
AU  - Celeska, Irena
AU  - Bojkovic-Kovacević, Slavica
AU  - Sladojević, Željko
AU  - Ivanov, Ivan
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1105
AB  - The aim of this study was to compare the concentrations of blood variables obtained simultaneously from the jugular and mammary veins of dairy cows. Eighty Holstein cows were divided into four equal groups: dry, low- (LY), medium- (MY) and high-yielding (HY). Blood insulin, glucose, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHBA) and urea concentrations were measured. The jugular and mammary vein (J/M) ratio between concentrations of each variable was calculated. Differences between the groups of cows in concentrations of variables in the jugular vein were not in accordance with those obtained for the mammary vein. J/M values for insulin and glucose concentrations were above 1.0 in all groups of cows. The ratios for NEFA and BHBA concentrations were under or equal to 1.0 in dry and LY cows but above 1.0 in the MY and HY groups, indicating that in MY and HY cows those metabolites are apparently utilised by the mammary gland. J/M values for urea were above 1.0 in dry and LY cows but less than 1.0 in groups MY and HY, indicating that in the latter case urea is apparently released by the mammary gland. In conclusion, J/M for NEFA, BHBA and urea may be useful for estimation of the critical point when the mammary gland receives insufficient energy precursors for its current activity.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - A comparison of the concentrations of energy-balance-related variables in jugular and mammary vein blood of dairy cows with different milk yield
VL  - 62
IS  - 1
SP  - 52
EP  - 63
DO  - 10.1556/AVet.2013.055
ER  - 

@article{
author = "Šamanc, Horea and Kirovski, Danijela and Lakić, Nada and Celeska, Irena and Bojkovic-Kovacević, Slavica and Sladojević, Željko and Ivanov, Ivan",
year = "2014",
abstract = "The aim of this study was to compare the concentrations of blood variables obtained simultaneously from the jugular and mammary veins of dairy cows. Eighty Holstein cows were divided into four equal groups: dry, low- (LY), medium- (MY) and high-yielding (HY). Blood insulin, glucose, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHBA) and urea concentrations were measured. The jugular and mammary vein (J/M) ratio between concentrations of each variable was calculated. Differences between the groups of cows in concentrations of variables in the jugular vein were not in accordance with those obtained for the mammary vein. J/M values for insulin and glucose concentrations were above 1.0 in all groups of cows. The ratios for NEFA and BHBA concentrations were under or equal to 1.0 in dry and LY cows but above 1.0 in the MY and HY groups, indicating that in MY and HY cows those metabolites are apparently utilised by the mammary gland. J/M values for urea were above 1.0 in dry and LY cows but less than 1.0 in groups MY and HY, indicating that in the latter case urea is apparently released by the mammary gland. In conclusion, J/M for NEFA, BHBA and urea may be useful for estimation of the critical point when the mammary gland receives insufficient energy precursors for its current activity.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "A comparison of the concentrations of energy-balance-related variables in jugular and mammary vein blood of dairy cows with different milk yield",
volume = "62",
number = "1",
pages = "52-63",
doi = "10.1556/AVet.2013.055"
}

Šamanc, H., Kirovski, D., Lakić, N., Celeska, I., Bojkovic-Kovacević, S., Sladojević, Ž.,& Ivanov, I.. (2014). A comparison of the concentrations of energy-balance-related variables in jugular and mammary vein blood of dairy cows with different milk yield. in Acta Veterinaria Hungarica
Akademiai Kiado Rt, Budapest., 62(1), 52-63.
https://doi.org/10.1556/AVet.2013.055

Šamanc H, Kirovski D, Lakić N, Celeska I, Bojkovic-Kovacević S, Sladojević Ž, Ivanov I. A comparison of the concentrations of energy-balance-related variables in jugular and mammary vein blood of dairy cows with different milk yield. in Acta Veterinaria Hungarica. 2014;62(1):52-63.
doi:10.1556/AVet.2013.055 .

Šamanc, Horea, Kirovski, Danijela, Lakić, Nada, Celeska, Irena, Bojkovic-Kovacević, Slavica, Sladojević, Željko, Ivanov, Ivan, "A comparison of the concentrations of energy-balance-related variables in jugular and mammary vein blood of dairy cows with different milk yield" in Acta Veterinaria Hungarica, 62, no. 1 (2014):52-63,
https://doi.org/10.1556/AVet.2013.055 . .

TY  - JOUR
AU  - Maletić, Milan
AU  - Vakanjac, Slobodanka
AU  - Đelić, Ninoslav
AU  - Lakić, Nada
AU  - Pavlović, Miloš
AU  - Nedić, Svetlana
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1057
AB  - Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes.
AB  - Laktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows
T1  - Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava
VL  - 63
IS  - 5-6
SP  - 487
EP  - 498
DO  - 10.2298/AVB1306487M
ER  - 

@article{
author = "Maletić, Milan and Vakanjac, Slobodanka and Đelić, Ninoslav and Lakić, Nada and Pavlović, Miloš and Nedić, Svetlana and Stanimirović, Zoran",
year = "2013",
abstract = "Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes., Laktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows, Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava",
volume = "63",
number = "5-6",
pages = "487-498",
doi = "10.2298/AVB1306487M"
}

Maletić, M., Vakanjac, S., Đelić, N., Lakić, N., Pavlović, M., Nedić, S.,& Stanimirović, Z.. (2013). Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 487-498.
https://doi.org/10.2298/AVB1306487M

Maletić M, Vakanjac S, Đelić N, Lakić N, Pavlović M, Nedić S, Stanimirović Z. Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows. in Acta Veterinaria-Beograd. 2013;63(5-6):487-498.
doi:10.2298/AVB1306487M .

Maletić, Milan, Vakanjac, Slobodanka, Đelić, Ninoslav, Lakić, Nada, Pavlović, Miloš, Nedić, Svetlana, Stanimirović, Zoran, "Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows" in Acta Veterinaria-Beograd, 63, no. 5-6 (2013):487-498,
https://doi.org/10.2298/AVB1306487M . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Stevanović, Jevrosima
AU  - Đelić, Ninoslav
AU  - Lakić, Nada
AU  - Knežević-Vukčević, Jelena
AU  - Vukovic-Gacić, Branka
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1037
AB  - Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.
PB  - Indian Acad Sciences, Bangalore
T2  - Journal of Biosciences
T1  - Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay
VL  - 38
IS  - 1
SP  - 53
EP  - 62
DO  - 10.1007/s12038-012-9287-2
ER  - 

@article{
author = "Radaković, Milena and Stevanović, Jevrosima and Đelić, Ninoslav and Lakić, Nada and Knežević-Vukčević, Jelena and Vukovic-Gacić, Branka and Stanimirović, Zoran",
year = "2013",
abstract = "Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.",
publisher = "Indian Acad Sciences, Bangalore",
journal = "Journal of Biosciences",
title = "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay",
volume = "38",
number = "1",
pages = "53-62",
doi = "10.1007/s12038-012-9287-2"
}

Radaković, M., Stevanović, J., Đelić, N., Lakić, N., Knežević-Vukčević, J., Vukovic-Gacić, B.,& Stanimirović, Z.. (2013). Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences
Indian Acad Sciences, Bangalore., 38(1), 53-62.
https://doi.org/10.1007/s12038-012-9287-2

Radaković M, Stevanović J, Đelić N, Lakić N, Knežević-Vukčević J, Vukovic-Gacić B, Stanimirović Z. Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences. 2013;38(1):53-62.
doi:10.1007/s12038-012-9287-2 .

Radaković, Milena, Stevanović, Jevrosima, Đelić, Ninoslav, Lakić, Nada, Knežević-Vukčević, Jelena, Vukovic-Gacić, Branka, Stanimirović, Zoran, "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay" in Journal of Biosciences, 38, no. 1 (2013):53-62,
https://doi.org/10.1007/s12038-012-9287-2 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Simeunović, Predrag
AU  - Gajić, Bojan
AU  - Lakić, Nada
AU  - Radović, Dejan
AU  - Fries, Ingemar
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1076
AB  - The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.
PB  - Springer France, Paris
T2  - Apidologie
T1  - Characteristics of Nosema ceranae infection in Serbian honey bee colonies
VL  - 44
IS  - 5
SP  - 522
EP  - 536
DO  - 10.1007/s13592-013-0203-z
ER  - 

@article{
author = "Stevanović, Jevrosima and Simeunović, Predrag and Gajić, Bojan and Lakić, Nada and Radović, Dejan and Fries, Ingemar and Stanimirović, Zoran",
year = "2013",
abstract = "The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.",
publisher = "Springer France, Paris",
journal = "Apidologie",
title = "Characteristics of Nosema ceranae infection in Serbian honey bee colonies",
volume = "44",
number = "5",
pages = "522-536",
doi = "10.1007/s13592-013-0203-z"
}

Stevanović, J., Simeunović, P., Gajić, B., Lakić, N., Radović, D., Fries, I.,& Stanimirović, Z.. (2013). Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie
Springer France, Paris., 44(5), 522-536.
https://doi.org/10.1007/s13592-013-0203-z

Stevanović J, Simeunović P, Gajić B, Lakić N, Radović D, Fries I, Stanimirović Z. Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie. 2013;44(5):522-536.
doi:10.1007/s13592-013-0203-z .

Stevanović, Jevrosima, Simeunović, Predrag, Gajić, Bojan, Lakić, Nada, Radović, Dejan, Fries, Ingemar, Stanimirović, Zoran, "Characteristics of Nosema ceranae infection in Serbian honey bee colonies" in Apidologie, 44, no. 5 (2013):522-536,
https://doi.org/10.1007/s13592-013-0203-z . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Kirovski, Danijela
AU  - Šamanc, Horea
AU  - Prodanović, Radiša
AU  - Lakić, Nada
AU  - Adamović, M.
AU  - Valčić, Olivera
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/885
AB  - The aim of this paper was to evaluate the effects of different environment temperatures on milk production in dairy cows under conditions typical for the moderate-continental climate. The trial was carried out on 40 Holstein Friesian dairy cows (20 cows in summer and 20 cows in the spring season). The cows were monitored from day 30 to day 90 of lactation and housed in a stable which during the summer was not equipped with additional cooling. The temperature-humidity index (THI) was recorded hourly. Milking was measured daily. Based on the results calculated for average all-day THI, during the spring season the cows were not exposed to heat stress. During the summer season, especially in the afternoon hours, the cows were exposed to the effects of moderate to severe heat stress. Average daily milk yield in the spring season from day 30 to day 90 of lactation was significantly higher (p  lt  0.001) compared to the same lactation period during the summer season. Nevertheless, there was no significant difference in average milk yield from day 30 to day 60 of lactation (p = 0.110) between spring and summer season. Average milk yield per cow from day 61 to day 90 of lactation in the summer period was significantly lower (p  lt  0.001) compared to the same period during the spring season. Average daily feed consumption was significantly lower (p  lt  0.001) during the summer compared to spring during whole trial period. In conclusion, although feed consumption was significantly lower during the summer season throught the study, milk production compared to the spring season, was significantly lower only from day 61 to 90 of lactation. This could mean that the negative impact of feed intake on milk production is not as effective during early lactation (until day 60 of lactation), when homeorhetic mechanisms play a significant role in the rearrangement of metabolism, as it is thereafter i.e.from day 61 to 90 of lactation.
PB  - Sivar-Soc Italiana Veterinari Animali Reddito, Cremona
T2  - Large Animal Review
T1  - Milk production in high-yielding dairy cows under different environment temperatures
VL  - 18
IS  - 1
SP  - 31
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_885
ER  - 

@article{
author = "Vujanac, Ivan and Kirovski, Danijela and Šamanc, Horea and Prodanović, Radiša and Lakić, Nada and Adamović, M. and Valčić, Olivera",
year = "2012",
abstract = "The aim of this paper was to evaluate the effects of different environment temperatures on milk production in dairy cows under conditions typical for the moderate-continental climate. The trial was carried out on 40 Holstein Friesian dairy cows (20 cows in summer and 20 cows in the spring season). The cows were monitored from day 30 to day 90 of lactation and housed in a stable which during the summer was not equipped with additional cooling. The temperature-humidity index (THI) was recorded hourly. Milking was measured daily. Based on the results calculated for average all-day THI, during the spring season the cows were not exposed to heat stress. During the summer season, especially in the afternoon hours, the cows were exposed to the effects of moderate to severe heat stress. Average daily milk yield in the spring season from day 30 to day 90 of lactation was significantly higher (p  lt  0.001) compared to the same lactation period during the summer season. Nevertheless, there was no significant difference in average milk yield from day 30 to day 60 of lactation (p = 0.110) between spring and summer season. Average milk yield per cow from day 61 to day 90 of lactation in the summer period was significantly lower (p  lt  0.001) compared to the same period during the spring season. Average daily feed consumption was significantly lower (p  lt  0.001) during the summer compared to spring during whole trial period. In conclusion, although feed consumption was significantly lower during the summer season throught the study, milk production compared to the spring season, was significantly lower only from day 61 to 90 of lactation. This could mean that the negative impact of feed intake on milk production is not as effective during early lactation (until day 60 of lactation), when homeorhetic mechanisms play a significant role in the rearrangement of metabolism, as it is thereafter i.e.from day 61 to 90 of lactation.",
publisher = "Sivar-Soc Italiana Veterinari Animali Reddito, Cremona",
journal = "Large Animal Review",
title = "Milk production in high-yielding dairy cows under different environment temperatures",
volume = "18",
number = "1",
pages = "31-36",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_885"
}

Vujanac, I., Kirovski, D., Šamanc, H., Prodanović, R., Lakić, N., Adamović, M.,& Valčić, O.. (2012). Milk production in high-yielding dairy cows under different environment temperatures. in Large Animal Review
Sivar-Soc Italiana Veterinari Animali Reddito, Cremona., 18(1), 31-36.
https://hdl.handle.net/21.15107/rcub_veterinar_885

Vujanac I, Kirovski D, Šamanc H, Prodanović R, Lakić N, Adamović M, Valčić O. Milk production in high-yielding dairy cows under different environment temperatures. in Large Animal Review. 2012;18(1):31-36.
https://hdl.handle.net/21.15107/rcub_veterinar_885 .

Vujanac, Ivan, Kirovski, Danijela, Šamanc, Horea, Prodanović, Radiša, Lakić, Nada, Adamović, M., Valčić, Olivera, "Milk production in high-yielding dairy cows under different environment temperatures" in Large Animal Review, 18, no. 1 (2012):31-36,
https://hdl.handle.net/21.15107/rcub_veterinar_885 .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Lakić, Nada
AU  - Đelić, Ninoslav
AU  - Radović, Ivica
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/882
AB  - The aim of this research was to investigate whether or not sugar dusting can stimulate the grooming behaviour in Apis mellifera L. (Hymenoptera: Apidae), an important defensive mechanism against Varroa destructor Anderson & Trueman (Acari: Varroidae), and to assess the most effective dose and frequency of treatment. The criterion for evaluation of grooming potential was the percentage of damaged mites (PDM) among the total number collected on the bottom boards of the hives. In each sugar-treated group PDM was significantly higher in comparison both with the negative control (no treatment) and with the values preceding the treatment. The results point to a stimulating effect of sugar on the grooming behaviour at all doses and frequencies tested. Treatment frequency influenced the stimulating effect of sugar: treatments at 3- and 7-day intervals with 30 and 40 g resulted in significantly higher PDMs than the least frequent treatment (every 14 days); dusting with 20 g influenced PDM only when repeated at 3-day intervals. Because treatments at 3-day intervals are time-consuming, those with 40 or 30 g repeated every 7 days may be recommended. In the positive control (hives treated with amitraz), average PDM was significantly lower than in the negative control and all sugar-treated groups. Possible causes of the stimulating effect of sugar dusting on bee grooming behaviour are discussed.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis et Applicata
T1  - Stimulating effect of sugar dusting on honey bee grooming behaviour
VL  - 143
IS  - 1
SP  - 23
EP  - 30
DO  - 10.1111/j.1570-7458.2012.01231.x
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Lakić, Nada and Đelić, Ninoslav and Radović, Ivica",
year = "2012",
abstract = "The aim of this research was to investigate whether or not sugar dusting can stimulate the grooming behaviour in Apis mellifera L. (Hymenoptera: Apidae), an important defensive mechanism against Varroa destructor Anderson & Trueman (Acari: Varroidae), and to assess the most effective dose and frequency of treatment. The criterion for evaluation of grooming potential was the percentage of damaged mites (PDM) among the total number collected on the bottom boards of the hives. In each sugar-treated group PDM was significantly higher in comparison both with the negative control (no treatment) and with the values preceding the treatment. The results point to a stimulating effect of sugar on the grooming behaviour at all doses and frequencies tested. Treatment frequency influenced the stimulating effect of sugar: treatments at 3- and 7-day intervals with 30 and 40 g resulted in significantly higher PDMs than the least frequent treatment (every 14 days); dusting with 20 g influenced PDM only when repeated at 3-day intervals. Because treatments at 3-day intervals are time-consuming, those with 40 or 30 g repeated every 7 days may be recommended. In the positive control (hives treated with amitraz), average PDM was significantly lower than in the negative control and all sugar-treated groups. Possible causes of the stimulating effect of sugar dusting on bee grooming behaviour are discussed.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis et Applicata",
title = "Stimulating effect of sugar dusting on honey bee grooming behaviour",
volume = "143",
number = "1",
pages = "23-30",
doi = "10.1111/j.1570-7458.2012.01231.x"
}

Stevanović, J., Stanimirović, Z., Lakić, N., Đelić, N.,& Radović, I.. (2012). Stimulating effect of sugar dusting on honey bee grooming behaviour. in Entomologia Experimentalis et Applicata
Wiley, Hoboken., 143(1), 23-30.
https://doi.org/10.1111/j.1570-7458.2012.01231.x

Stevanović J, Stanimirović Z, Lakić N, Đelić N, Radović I. Stimulating effect of sugar dusting on honey bee grooming behaviour. in Entomologia Experimentalis et Applicata. 2012;143(1):23-30.
doi:10.1111/j.1570-7458.2012.01231.x .

Stevanović, Jevrosima, Stanimirović, Zoran, Lakić, Nada, Đelić, Ninoslav, Radović, Ivica, "Stimulating effect of sugar dusting on honey bee grooming behaviour" in Entomologia Experimentalis et Applicata, 143, no. 1 (2012):23-30,
https://doi.org/10.1111/j.1570-7458.2012.01231.x . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Simeunović, Predrag
AU  - Kulišić, Zoran
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/760
AB  - The hygienic behavior in honey bees is a dominant natural defense mechanism against brood diseases. In this study, the influence of sugar dusting treatments on hygienic behavior was evaluated in 44 strong honey bee colonies. Three doses of pulverized sugar, 20, 30 and 40 g, each applied at three-, seven- and fourteen-day intervals were tested. The percentage of cleaned cells (PCC) in the total number of those with pin-killed brood served as a measure of the hygienic potential. The effect was dependent on the frequency of treatments: all doses applied every third and seventh day significantly (p lt 0.001) decreased the PCC in comparison with the untreated control colonies. Nevertheless, sugar did not threaten the hygienic potential, as PPC values remained above 94% following all treatments. Thus, it can be concluded that the tested sugar treatments are safe and can be justifiably implemented into integrated pest management strategies to control Varroa destructor.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies
VL  - 63
IS  - 4
SP  - 1199
EP  - 1207
DO  - 10.2298/ABS1104199S
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Lakić, Nada and Aleksić, Nevenka and Simeunović, Predrag and Kulišić, Zoran",
year = "2011",
abstract = "The hygienic behavior in honey bees is a dominant natural defense mechanism against brood diseases. In this study, the influence of sugar dusting treatments on hygienic behavior was evaluated in 44 strong honey bee colonies. Three doses of pulverized sugar, 20, 30 and 40 g, each applied at three-, seven- and fourteen-day intervals were tested. The percentage of cleaned cells (PCC) in the total number of those with pin-killed brood served as a measure of the hygienic potential. The effect was dependent on the frequency of treatments: all doses applied every third and seventh day significantly (p lt 0.001) decreased the PCC in comparison with the untreated control colonies. Nevertheless, sugar did not threaten the hygienic potential, as PPC values remained above 94% following all treatments. Thus, it can be concluded that the tested sugar treatments are safe and can be justifiably implemented into integrated pest management strategies to control Varroa destructor.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies",
volume = "63",
number = "4",
pages = "1199-1207",
doi = "10.2298/ABS1104199S"
}

Stevanović, J., Stanimirović, Z., Lakić, N., Aleksić, N., Simeunović, P.,& Kulišić, Z.. (2011). Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(4), 1199-1207.
https://doi.org/10.2298/ABS1104199S

Stevanović J, Stanimirović Z, Lakić N, Aleksić N, Simeunović P, Kulišić Z. Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies. in Archives of Biological Sciences. 2011;63(4):1199-1207.
doi:10.2298/ABS1104199S .

Stevanović, Jevrosima, Stanimirović, Zoran, Lakić, Nada, Aleksić, Nevenka, Simeunović, Predrag, Kulišić, Zoran, "Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies" in Archives of Biological Sciences, 63, no. 4 (2011):1199-1207,
https://doi.org/10.2298/ABS1104199S . .