TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Ðordević, Igor
AU  - Anđelković, Katarina
AU  - Petković, Jelena
AU  - Dačić, Miroljub
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2540
AB  - The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022
PB  - MDPI
T2  - Pathogens
T1  - Patterns of ASFV Transmission in Domestic Pigs in Serbia
VL  - 12
IS  - 149
DO  - 10.3390/pathogens12010149
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Veljović, Ljubiša and Milovanović, Bojan and Kureljušić, Branislav and Ðordević, Igor and Anđelković, Katarina and Petković, Jelena and Dačić, Miroljub",
year = "2023",
abstract = "The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022",
publisher = "MDPI",
journal = "Pathogens",
title = "Patterns of ASFV Transmission in Domestic Pigs in Serbia",
volume = "12",
number = "149",
doi = "10.3390/pathogens12010149"
}

Glišić, D., Milićević, V., Veljović, L., Milovanović, B., Kureljušić, B., Ðordević, I., Anđelković, K., Petković, J.,& Dačić, M.. (2023). Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens
MDPI., 12(149).
https://doi.org/10.3390/pathogens12010149

Glišić D, Milićević V, Veljović L, Milovanović B, Kureljušić B, Ðordević I, Anđelković K, Petković J, Dačić M. Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens. 2023;12(149).
doi:10.3390/pathogens12010149 .

Glišić, Dimitrije, Milićević, Vesna, Veljović, Ljubiša, Milovanović, Bojan, Kureljušić, Branislav, Ðordević, Igor, Anđelković, Katarina, Petković, Jelena, Dačić, Miroljub, "Patterns of ASFV Transmission in Domestic Pigs in Serbia" in Pathogens, 12, no. 149 (2023),
https://doi.org/10.3390/pathogens12010149 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Mirilović, Milorad
AU  - Vejnović, Branislav
AU  - Ćosić, Milivoje
AU  - Knežević, Aleksandra
AU  - Veljović, Ljubiša
AU  - Živulj, Aleksandar
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2357
AB  - The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.
T2  - Veterinarni Medicina
T1  - Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia
VL  - 67
DO  - 10.17221/32/2021-VETMED
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Mirilović, Milorad and Vejnović, Branislav and Ćosić, Milivoje and Knežević, Aleksandra and Veljović, Ljubiša and Živulj, Aleksandar",
year = "2022",
abstract = "The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.",
journal = "Veterinarni Medicina",
title = "Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia",
volume = "67",
doi = "10.17221/32/2021-VETMED"
}

Nišavić, J., Milić, N., Radalj, A., Mirilović, M., Vejnović, B., Ćosić, M., Knežević, A., Veljović, L.,& Živulj, A.. (2022). Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia. in Veterinarni Medicina, 67.
https://doi.org/10.17221/32/2021-VETMED

Nišavić J, Milić N, Radalj A, Mirilović M, Vejnović B, Ćosić M, Knežević A, Veljović L, Živulj A. Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia. in Veterinarni Medicina. 2022;67.
doi:10.17221/32/2021-VETMED .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Mirilović, Milorad, Vejnović, Branislav, Ćosić, Milivoje, Knežević, Aleksandra, Veljović, Ljubiša, Živulj, Aleksandar, "Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia" in Veterinarni Medicina, 67 (2022),
https://doi.org/10.17221/32/2021-VETMED . .

TY  - JOUR
AU  - Radosavljević, Vladimir
AU  - Zdravković, Nemanja
AU  - Veljović, Ljubiša
AU  - Radanović, Oliver
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2709
AB  - Global freshwater fish production in aquaculture has grown rapidly in recent decades. This constant growth, involving novel forms of intensive aquaculture, has increased global  movements  of  fish  and  boosted  various  anthropogenic  stresses  to  aquatic ecosystems, so rainbow trout aquaculture has encountered the emergence and outbreaks of many bacterial diseases. Due to the need to effectively prevent and control disease outbreaks, vaccines have become an important technology in intensive trout aquaculture. In this review, the applications of specific vaccines against important bacterial diseases of rainbow trout in Serbian aquaculture are summarized.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Important bacterial diseases and their control in rainbow trout in Serbian aquaculture
VL  - 76
IS  - 2
SP  - 103
SP  - 112
DO  - 10.2298/VETGL211217006R
ER  - 

@article{
author = "Radosavljević, Vladimir and Zdravković, Nemanja and Veljović, Ljubiša and Radanović, Oliver",
year = "2022",
abstract = "Global freshwater fish production in aquaculture has grown rapidly in recent decades. This constant growth, involving novel forms of intensive aquaculture, has increased global  movements  of  fish  and  boosted  various  anthropogenic  stresses  to  aquatic ecosystems, so rainbow trout aquaculture has encountered the emergence and outbreaks of many bacterial diseases. Due to the need to effectively prevent and control disease outbreaks, vaccines have become an important technology in intensive trout aquaculture. In this review, the applications of specific vaccines against important bacterial diseases of rainbow trout in Serbian aquaculture are summarized.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Important bacterial diseases and their control in rainbow trout in Serbian aquaculture",
volume = "76",
number = "2",
pages = "103-112",
doi = "10.2298/VETGL211217006R"
}

Radosavljević, V., Zdravković, N., Veljović, L.,& Radanović, O.. (2022). Important bacterial diseases and their control in rainbow trout in Serbian aquaculture. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 76(2), 103.
https://doi.org/10.2298/VETGL211217006R

Radosavljević V, Zdravković N, Veljović L, Radanović O. Important bacterial diseases and their control in rainbow trout in Serbian aquaculture. in Veterinarski Glasnik. 2022;76(2):103.
doi:10.2298/VETGL211217006R .

Radosavljević, Vladimir, Zdravković, Nemanja, Veljović, Ljubiša, Radanović, Oliver, "Important bacterial diseases and their control in rainbow trout in Serbian aquaculture" in Veterinarski Glasnik, 76, no. 2 (2022):103,
https://doi.org/10.2298/VETGL211217006R . .

TY  - JOUR
AU  - Radalj, Andrea
AU  - Milić, Nenad
AU  - Stevanović, Oliver
AU  - Veljović, Ljubiša
AU  - Nišavić, Jakov
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2258
AB  - Equine herpesvirus 1 (EHV-1) causes considerable economic loss to the equine industry and is spread among susceptible animals during the cycles of latency and reactivation, causing rhinopneumonitis, abortion, and neurological disease. Nucleotide polymorphisms within ORF30 and ORF68 sequences of the viral genome are associated with strain neuropathogenicity and geographical origin. A total of 142 tissue and nasal swab samples from apparently healthy unvaccinated horses were examined to ascertain EHV-1 distribution, diversity, and clinical significance considering the results of virus isolation, sequence analysis, and anamnestic data. The ORF30 and ORF68 molecular study of these circulating strains and archival isolates from abortion storms aimed to contribute to the perception of strain pathogenicity and origin. EHV-1 was detected by PCR and virus isolation in 81 and 45.1% of the analyzed samples, respectively, and 82.1% of the representative samples were neuropathogenic strains. The ORF68-based grouping was restricted by the pronounced polymorphism of Balkan EHV-1 strains, and only two isolates were assigned to group 4. The cases of abortion were caused by neuropathogenic strains that also circulate within the horse population with no documented outbreaks of disease. It was evident that strain virulence is not solely accountable for the development of clinical symptoms in affected animals. Neural tissue is significant for virus latency and reactivation, considering the number of EHV-1 isolates from apparently healthy stressed horses. Special care must be taken when accommodating together immunologically naive and latently infected horses since asymptomatic carriers silently shed EHV-1.
PB  - University of Agriculture, Faisalabad in Pakistan
T2  - Pakistan Veterinary Journal
T1  - Genetic Characterization of Equine Herpesvirus 1 from Clinical Cases and Asymptomatic Horses in Serbia and Bosnia and Herzegovina
VL  - 41
IS  - 4
SP  - 567
EP  - 573
DO  - 10.29261/pakvetj/2021.062
ER  - 

@article{
author = "Radalj, Andrea and Milić, Nenad and Stevanović, Oliver and Veljović, Ljubiša and Nišavić, Jakov",
year = "2021",
abstract = "Equine herpesvirus 1 (EHV-1) causes considerable economic loss to the equine industry and is spread among susceptible animals during the cycles of latency and reactivation, causing rhinopneumonitis, abortion, and neurological disease. Nucleotide polymorphisms within ORF30 and ORF68 sequences of the viral genome are associated with strain neuropathogenicity and geographical origin. A total of 142 tissue and nasal swab samples from apparently healthy unvaccinated horses were examined to ascertain EHV-1 distribution, diversity, and clinical significance considering the results of virus isolation, sequence analysis, and anamnestic data. The ORF30 and ORF68 molecular study of these circulating strains and archival isolates from abortion storms aimed to contribute to the perception of strain pathogenicity and origin. EHV-1 was detected by PCR and virus isolation in 81 and 45.1% of the analyzed samples, respectively, and 82.1% of the representative samples were neuropathogenic strains. The ORF68-based grouping was restricted by the pronounced polymorphism of Balkan EHV-1 strains, and only two isolates were assigned to group 4. The cases of abortion were caused by neuropathogenic strains that also circulate within the horse population with no documented outbreaks of disease. It was evident that strain virulence is not solely accountable for the development of clinical symptoms in affected animals. Neural tissue is significant for virus latency and reactivation, considering the number of EHV-1 isolates from apparently healthy stressed horses. Special care must be taken when accommodating together immunologically naive and latently infected horses since asymptomatic carriers silently shed EHV-1.",
publisher = "University of Agriculture, Faisalabad in Pakistan",
journal = "Pakistan Veterinary Journal",
title = "Genetic Characterization of Equine Herpesvirus 1 from Clinical Cases and Asymptomatic Horses in Serbia and Bosnia and Herzegovina",
volume = "41",
number = "4",
pages = "567-573",
doi = "10.29261/pakvetj/2021.062"
}

Radalj, A., Milić, N., Stevanović, O., Veljović, L.,& Nišavić, J.. (2021). Genetic Characterization of Equine Herpesvirus 1 from Clinical Cases and Asymptomatic Horses in Serbia and Bosnia and Herzegovina. in Pakistan Veterinary Journal
University of Agriculture, Faisalabad in Pakistan., 41(4), 567-573.
https://doi.org/10.29261/pakvetj/2021.062

Radalj A, Milić N, Stevanović O, Veljović L, Nišavić J. Genetic Characterization of Equine Herpesvirus 1 from Clinical Cases and Asymptomatic Horses in Serbia and Bosnia and Herzegovina. in Pakistan Veterinary Journal. 2021;41(4):567-573.
doi:10.29261/pakvetj/2021.062 .

Radalj, Andrea, Milić, Nenad, Stevanović, Oliver, Veljović, Ljubiša, Nišavić, Jakov, "Genetic Characterization of Equine Herpesvirus 1 from Clinical Cases and Asymptomatic Horses in Serbia and Bosnia and Herzegovina" in Pakistan Veterinary Journal, 41, no. 4 (2021):567-573,
https://doi.org/10.29261/pakvetj/2021.062 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Stanojković, Aleksandar
AU  - Veljović, Ljubiša
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2260
AB  - The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.
PB  - Belgrade : Institute for Animal Husbandry
T2  - Biotechnology in Animal Husbandry
T1  - Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease
T1  - Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda
VL  - 37
IS  - 1
SP  - 1
EP  - 15
DO  - 10.2298/BAH2101001N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Stanojković, Aleksandar and Veljović, Ljubiša",
year = "2021",
abstract = "The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "Biotechnology in Animal Husbandry",
title = "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease, Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda",
volume = "37",
number = "1",
pages = "1-15",
doi = "10.2298/BAH2101001N"
}

Nišavić, J., Milić, N., Radalj, A., Stanojković, A.,& Veljović, L.. (2021). Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry
Belgrade : Institute for Animal Husbandry., 37(1), 1-15.
https://doi.org/10.2298/BAH2101001N

Nišavić J, Milić N, Radalj A, Stanojković A, Veljović L. Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry. 2021;37(1):1-15.
doi:10.2298/BAH2101001N .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Stanojković, Aleksandar, Veljović, Ljubiša, "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease" in Biotechnology in Animal Husbandry, 37, no. 1 (2021):1-15,
https://doi.org/10.2298/BAH2101001N . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Savić, Božidar
AU  - Radojičić, Sonja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1793
AB  - Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The performance of seven molecular methods for the detection of prrsv
VL  - 70
IS  - 1
SP  - 51
EP  - 57
DO  - 10.2478/acve-2020-0003
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Veljović, Ljubiša and Valčić, Miroslav and Stević, Nataša and Savić, Božidar and Radojičić, Sonja",
year = "2020",
abstract = "Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The performance of seven molecular methods for the detection of prrsv",
volume = "70",
number = "1",
pages = "51-57",
doi = "10.2478/acve-2020-0003"
}

Milićević, V., Kureljušić, B., Veljović, L., Valčić, M., Stević, N., Savić, B.,& Radojičić, S.. (2020). The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 51-57.
https://doi.org/10.2478/acve-2020-0003

Milićević V, Kureljušić B, Veljović L, Valčić M, Stević N, Savić B, Radojičić S. The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd. 2020;70(1):51-57.
doi:10.2478/acve-2020-0003 .

Milićević, Vesna, Kureljušić, Branislav, Veljović, Ljubiša, Valčić, Miroslav, Stević, Nataša, Savić, Božidar, Radojičić, Sonja, "The performance of seven molecular methods for the detection of prrsv" in Acta Veterinaria-Beograd, 70, no. 1 (2020):51-57,
https://doi.org/10.2478/acve-2020-0003 . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1796
AB  - A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.
PB  - Polish Soc Veterinary Sciences Editorial Office, Lublin
T2  - Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.",
publisher = "Polish Soc Veterinary Sciences Editorial Office, Lublin",
journal = "Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
Polish Soc Veterinary Sciences Editorial Office, Lublin., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2017
AB  - A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.
PB  - Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)
T2  - Medycyna Weterynaryjna
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.",
publisher = "Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)",
journal = "Medycyna Weterynaryjna",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna
Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Pérez-Ramírez, Elisa
AU  - Cano-Gómez, Cristina
AU  - Llorente, Francisco
AU  - Vodica, Ani
AU  - Veljović, Ljubiša
AU  - Toklikishvilli, Natela
AU  - Sherifi, Kurtesh
AU  - Sghaier, Soufien
AU  - Omani, Amel
AU  - Kustura, Aida
AU  - Krstevski, Kiril
AU  - Karayel-Hacioglu, Ilke
AU  - Hagag, Naglaa Mohamed
AU  - Hage, Jeanne El
AU  - Davdyan, Hasmik
AU  - Bintarif, Mohd Saddam
AU  - Adžić, Bojan
AU  - Abouchoaib, Nabil
AU  - Jiménez-Clavero, Miguel Ángel
AU  - Fernández-Pinero, Jovita
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2019
AB  - The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.
PB  - MDPI AG
T2  - Pathogens
T1  - Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions
VL  - 9
IS  - 12
SP  - 1
EP  - 20
DO  - 10.3390/pathogens9121038
ER  - 

@article{
author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Vodica, Ani and Veljović, Ljubiša and Toklikishvilli, Natela and Sherifi, Kurtesh and Sghaier, Soufien and Omani, Amel and Kustura, Aida and Krstevski, Kiril and Karayel-Hacioglu, Ilke and Hagag, Naglaa Mohamed and Hage, Jeanne El and Davdyan, Hasmik and Bintarif, Mohd Saddam and Adžić, Bojan and Abouchoaib, Nabil and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita",
year = "2020",
abstract = "The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.",
publisher = "MDPI AG",
journal = "Pathogens",
title = "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions",
volume = "9",
number = "12",
pages = "1-20",
doi = "10.3390/pathogens9121038"
}

Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Vodica, A., Veljović, L., Toklikishvilli, N., Sherifi, K., Sghaier, S., Omani, A., Kustura, A., Krstevski, K., Karayel-Hacioglu, I., Hagag, N. M., Hage, J. E., Davdyan, H., Bintarif, M. S., Adžić, B., Abouchoaib, N., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens
MDPI AG., 9(12), 1-20.
https://doi.org/10.3390/pathogens9121038

Pérez-Ramírez E, Cano-Gómez C, Llorente F, Vodica A, Veljović L, Toklikishvilli N, Sherifi K, Sghaier S, Omani A, Kustura A, Krstevski K, Karayel-Hacioglu I, Hagag NM, Hage JE, Davdyan H, Bintarif MS, Adžić B, Abouchoaib N, Jiménez-Clavero MÁ, Fernández-Pinero J. Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens. 2020;9(12):1-20.
doi:10.3390/pathogens9121038 .

Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Vodica, Ani, Veljović, Ljubiša, Toklikishvilli, Natela, Sherifi, Kurtesh, Sghaier, Soufien, Omani, Amel, Kustura, Aida, Krstevski, Kiril, Karayel-Hacioglu, Ilke, Hagag, Naglaa Mohamed, Hage, Jeanne El, Davdyan, Hasmik, Bintarif, Mohd Saddam, Adžić, Bojan, Abouchoaib, Nabil, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions" in Pathogens, 9, no. 12 (2020):1-20,
https://doi.org/10.3390/pathogens9121038 . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Maksimović-Zorić, Jelena
AU  - Stanojević, Slobodan
AU  - Radanović, Oliver
AU  - Đurić, Boban
AU  - Plavšić, Budimir
AU  - Vasić, Ana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1958
AB  - Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females.
AB  - Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.
PB  - Scientific Veterinary Institute “Novi Sad”, Novi Sad
T2  - Archives of Veterinary Medicine
T1  - Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016
T1  - Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine
VL  - 10
IS  - 1
DO  - 10.46784/e-avm.v10i1.76
ER  - 

@article{
author = "Pavlović, Ivan and Veljović, Ljubiša and Milićević, Vesna and Maksimović-Zorić, Jelena and Stanojević, Slobodan and Radanović, Oliver and Đurić, Boban and Plavšić, Budimir and Vasić, Ana",
year = "2019",
abstract = "Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females., Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.",
publisher = "Scientific Veterinary Institute “Novi Sad”, Novi Sad",
journal = "Archives of Veterinary Medicine",
title = "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016, Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine",
volume = "10",
number = "1",
doi = "10.46784/e-avm.v10i1.76"
}

Pavlović, I., Veljović, L., Milićević, V., Maksimović-Zorić, J., Stanojević, S., Radanović, O., Đurić, B., Plavšić, B.,& Vasić, A.. (2019). Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”, Novi Sad., 10(1).
https://doi.org/10.46784/e-avm.v10i1.76

Pavlović I, Veljović L, Milićević V, Maksimović-Zorić J, Stanojević S, Radanović O, Đurić B, Plavšić B, Vasić A. Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine. 2019;10(1).
doi:10.46784/e-avm.v10i1.76 .

Pavlović, Ivan, Veljović, Ljubiša, Milićević, Vesna, Maksimović-Zorić, Jelena, Stanojević, Slobodan, Radanović, Oliver, Đurić, Boban, Plavšić, Budimir, Vasić, Ana, "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016" in Archives of Veterinary Medicine, 10, no. 1 (2019),
https://doi.org/10.46784/e-avm.v10i1.76 . .

TY  - JOUR
AU  - Radalj, Andrea
AU  - Nišavić, Jakov
AU  - Krnjaić, Dejan
AU  - Valčić, Miroslav
AU  - Jovanović, Tanja
AU  - Veljović, Ljubiša
AU  - Milić, Nenad
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1626
AB  - The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria - Brno
T1  - Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia
VL  - 87
IS  - 1
SP  - 27
DO  - 10.2754/avb201887010027
ER  - 

@article{
author = "Radalj, Andrea and Nišavić, Jakov and Krnjaić, Dejan and Valčić, Miroslav and Jovanović, Tanja and Veljović, Ljubiša and Milić, Nenad",
year = "2018",
abstract = "The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria - Brno",
title = "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia",
volume = "87",
number = "1",
pages = "27",
doi = "10.2754/avb201887010027"
}

Radalj, A., Nišavić, J., Krnjaić, D., Valčić, M., Jovanović, T., Veljović, L.,& Milić, N.. (2018). Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(1), 27.
https://doi.org/10.2754/avb201887010027

Radalj A, Nišavić J, Krnjaić D, Valčić M, Jovanović T, Veljović L, Milić N. Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno. 2018;87(1):27.
doi:10.2754/avb201887010027 .

Radalj, Andrea, Nišavić, Jakov, Krnjaić, Dejan, Valčić, Miroslav, Jovanović, Tanja, Veljović, Ljubiša, Milić, Nenad, "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia" in Acta Veterinaria - Brno, 87, no. 1 (2018):27,
https://doi.org/10.2754/avb201887010027 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Kureljušić, Jasna
AU  - Šekler, Milanko
AU  - Nešić, Ksenija
AU  - Veljović, Ljubiša
AU  - Zorić-Maksimović, Jelena
AU  - Radosavljević, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1657
AB  - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.
PB  - Springeropen, London
T2  - AMB EXPRESS
T1  - Molecular detection of black queen cell virus and Kashmir bee virus in honey
VL  - 8
SP  - 128
DO  - 10.1186/s13568-018-0655-7
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Zorić-Maksimović, Jelena and Radosavljević, Vladimir",
year = "2018",
abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.",
publisher = "Springeropen, London",
journal = "AMB EXPRESS",
title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey",
volume = "8",
pages = "128",
doi = "10.1186/s13568-018-0655-7"
}

Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Zorić-Maksimović, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS
Springeropen, London., 8, 128.
https://doi.org/10.1186/s13568-018-0655-7

Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Zorić-Maksimović J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS. 2018;8:128.
doi:10.1186/s13568-018-0655-7 .

Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Zorić-Maksimović, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB EXPRESS, 8 (2018):128,
https://doi.org/10.1186/s13568-018-0655-7 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Radosavljević, Vladimir
AU  - Jezdimirović, Nemanja
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1564
AB  - Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever.
AB  - Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Some adverse events following immunization in veterinary medicine
T1  - Procena neželjenih reakcija nakon vakcinacije u veterini
VL  - 11
IS  - 1
SP  - 67
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1564
ER  - 

@article{
author = "Milićević, Vesna and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Žutić, Jadranka and Kureljušić, Branislav and Radosavljević, Vladimir and Jezdimirović, Nemanja and Radojičić, Sonja",
year = "2018",
abstract = "Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever., Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Some adverse events following immunization in veterinary medicine, Procena neželjenih reakcija nakon vakcinacije u veterini",
volume = "11",
number = "1",
pages = "67-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1564"
}

Milićević, V., Veljović, L., Maksimović-Zorić, J., Žutić, J., Kureljušić, B., Radosavljević, V., Jezdimirović, N.,& Radojičić, S.. (2018). Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 11(1), 67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564

Milićević V, Veljović L, Maksimović-Zorić J, Žutić J, Kureljušić B, Radosavljević V, Jezdimirović N, Radojičić S. Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine. 2018;11(1):67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

Milićević, Vesna, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Žutić, Jadranka, Kureljušić, Branislav, Radosavljević, Vladimir, Jezdimirović, Nemanja, Radojičić, Sonja, "Some adverse events following immunization in veterinary medicine" in Arhiv veterinarske medicine, 11, no. 1 (2018):67-77,
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radosavljević, Vladimir
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1450
AB  - African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia.
AB  - Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Epizootiological situation of African swine fever in Europe
T1  - Epizootiološka situacija afričke kuge svinja u Evropi
VL  - 10
IS  - 2
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1450
ER  - 

@article{
author = "Milićević, Vesna and Radosavljević, Vladimir and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Radojičić, Sonja",
year = "2017",
abstract = "African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia., Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Epizootiological situation of African swine fever in Europe, Epizootiološka situacija afričke kuge svinja u Evropi",
volume = "10",
number = "2",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1450"
}

Milićević, V., Radosavljević, V., Veljović, L., Maksimović-Zorić, J.,& Radojičić, S.. (2017). Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 10(2), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450

Milićević V, Radosavljević V, Veljović L, Maksimović-Zorić J, Radojičić S. Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine. 2017;10(2):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .

Milićević, Vesna, Radosavljević, Vladimir, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Radojičić, Sonja, "Epizootiological situation of African swine fever in Europe" in Arhiv veterinarske medicine, 10, no. 2 (2017):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .

TY  - JOUR
AU  - Lukač, Bojan
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Đurić, Spomenka
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1352
AB  - The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.
AB  - Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina
T1  - Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina
VL  - 66
IS  - 1
SP  - 51
EP  - 60
DO  - 10.1515/acve-2016-0004
ER  - 

@article{
author = "Lukač, Bojan and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Đurić, Spomenka and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China., Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina, Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina",
volume = "66",
number = "1",
pages = "51-60",
doi = "10.1515/acve-2016-0004"
}

Lukač, B., Knežević, A., Milić, N., Krnjaić, D., Veljović, L., Milićević, V., Zorić, A., Đurić, S., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 51-60.
https://doi.org/10.1515/acve-2016-0004

Lukač B, Knežević A, Milić N, Krnjaić D, Veljović L, Milićević V, Zorić A, Đurić S, Stanojević M, Nišavić J. Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd. 2016;66(1):51-60.
doi:10.1515/acve-2016-0004 .

Lukač, Bojan, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Đurić, Spomenka, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina" in Acta Veterinaria-Beograd, 66, no. 1 (2016):51-60,
https://doi.org/10.1515/acve-2016-0004 . .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Miković, Radoš
AU  - Zorić, Andrea
AU  - Marković, Maja
AU  - Milićević, Vesna
AU  - Stamenković, Miodrag
AU  - Stanojević, Maja
AU  - Maksimović-Zorić, Jelena
AU  - Petrović, Tamaš
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1368
AB  - The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
AB  - Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia
T1  - Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji
VL  - 66
IS  - 4
SP  - 509
EP  - 519
DO  - 10.1515/acve-2016-0044
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Miković, Radoš and Zorić, Andrea and Marković, Maja and Milićević, Vesna and Stamenković, Miodrag and Stanojević, Maja and Maksimović-Zorić, Jelena and Petrović, Tamaš and Nišavić, Jakov",
year = "2016",
abstract = "The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank., Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia, Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji",
volume = "66",
number = "4",
pages = "509-519",
doi = "10.1515/acve-2016-0044"
}

Veljović, L., Knežević, A., Milić, N., Krnjaić, D., Miković, R., Zorić, A., Marković, M., Milićević, V., Stamenković, M., Stanojević, M., Maksimović-Zorić, J., Petrović, T.,& Nišavić, J.. (2016). Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 509-519.
https://doi.org/10.1515/acve-2016-0044

Veljović L, Knežević A, Milić N, Krnjaić D, Miković R, Zorić A, Marković M, Milićević V, Stamenković M, Stanojević M, Maksimović-Zorić J, Petrović T, Nišavić J. Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd. 2016;66(4):509-519.
doi:10.1515/acve-2016-0044 .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Miković, Radoš, Zorić, Andrea, Marković, Maja, Milićević, Vesna, Stamenković, Miodrag, Stanojević, Maja, Maksimović-Zorić, Jelena, Petrović, Tamaš, Nišavić, Jakov, "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia" in Acta Veterinaria-Beograd, 66, no. 4 (2016):509-519,
https://doi.org/10.1515/acve-2016-0044 . .

TY  - JOUR
AU  - Zorić-Maksimović, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Pavlović, Ivan
AU  - Radosavljević, Vladimir
AU  - Valčić, Miroslav
AU  - Glišić, Mileva
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1329
AB  - Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus.
AB  - Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis
T1  - Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika
VL  - 9
IS  - 1
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1329
ER  - 

@article{
author = "Zorić-Maksimović, Jelena and Milićević, Vesna and Veljović, Ljubiša and Pavlović, Ivan and Radosavljević, Vladimir and Valčić, Miroslav and Glišić, Mileva",
year = "2016",
abstract = "Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus., Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis, Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika",
volume = "9",
number = "1",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1329"
}

Zorić-Maksimović, J., Milićević, V., Veljović, L., Pavlović, I., Radosavljević, V., Valčić, M.,& Glišić, M.. (2016). Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(1), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329

Zorić-Maksimović J, Milićević V, Veljović L, Pavlović I, Radosavljević V, Valčić M, Glišić M. Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine. 2016;9(1):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

Zorić-Maksimović, Jelena, Milićević, Vesna, Veljović, Ljubiša, Pavlović, Ivan, Radosavljević, Vladimir, Valčić, Miroslav, Glišić, Mileva, "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis" in Arhiv veterinarske medicine, 9, no. 1 (2016):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1092
AB  - Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.
PB  - Srpsko biološko društvo, Beograd
T2  - Archives of Biological Sciences
T1  - The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle
VL  - 66
IS  - 2
SP  - 491
EP  - 496
DO  - 10.2298/ABS1402491V
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Nišavić, Jakov",
year = "2014",
abstract = "Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "Archives of Biological Sciences",
title = "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle",
volume = "66",
number = "2",
pages = "491-496",
doi = "10.2298/ABS1402491V"
}

Veljović, L., Knežević, A., Milić, N.,& Nišavić, J.. (2014). The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd., 66(2), 491-496.
https://doi.org/10.2298/ABS1402491V

Veljović L, Knežević A, Milić N, Nišavić J. The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences. 2014;66(2):491-496.
doi:10.2298/ABS1402491V .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Nišavić, Jakov, "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle" in Archives of Biological Sciences, 66, no. 2 (2014):491-496,
https://doi.org/10.2298/ABS1402491V . .

TY  - JOUR
AU  - Maksimović-Zorić, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Petrović, Tamaš
AU  - Valčić, Miroslav
AU  - Plavšić, Branislav
AU  - Vranješ, N.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/994
AB  - Rabies is a disease that occurs both in animals and people, with relatively long period of incubation, intense clinical course and fatal ending. Majority of cases of illnes and death in people are caused by the standard rabies virus. According to books of regulations on suppression and eradiction of rabies from 1988. and 2009., each suspected rabies has to be laboratory confirmed. From 2006. to 2012. within the passive surveilance, 3549 samples of brain tissue were examined for the presence of rabies virus. The virus was confirmed in 923 samples by using the method of direct imunofluorescence. From 2006. to 2012. there were diagnosed 192, 160, 233, 181, 104, 43 and 10 positive cases, respectively. For the purpose of rabies eradiction at the territory of the Republic of Serbia, a regional project of oral vaccination of foxes against rabies has been implemented since 2010.Within monitoring of the effectiveness of oral vaccination against rabies which was carried out at the end of 2011. and at the beginning of 2012., there were examined 1385 samples, out of which 11 reacted positively. The virus was dominantly present in the fox population. During 2008. and 2009., a relatively large number of rabid cats was registered, what indicates a transmission of rabies from the fox to the cat population. The incidence of rabies at the territory of Serbia significantly differs from one epizootic area to another. Northern parts of the country (Sombor and Subotica epizootic regions) have more favorable situation with only two cases of rabies for the last 7 years. In the period until 2010., in Pozarevac, Novi Sad, Jagodina, Pancevo, Belgrade and Nis epizootic region, a gradual decline in number of positive cases was noticed. International project for oral vaccination of foxes against rabies which has been implemented at the territory of the Republic of Serbia and neighboring countries, influenced a significant reduction of registered rabies cases.
AB  - Besnilo je bolest životinja i ljudi koja se odlikuje relativno dugim inkubacionim periodom i dramatičnim kliničkim tokom, koji se uvek završava smrtnim ishodom. Najveći broj slučajeva oboljenja i smrti ljudi potiče od infekcije klasičnim virusom besnila. Prema pravilnicima koji se bave suzbijanjem i iskorenjivanjem besnila iz 1988. i 2009. godine obavezna je laboratorijska potvrda svake klinički opravdane sumnje na besnilo. Od 2006. do 2012. godine u okviru pasivnog nadzora pregledano je 3549 uzoraka moždanog tkiva na prisustvo virusa besnila. Metodom direktne imunofluorescencije virus je dokazan u 923 uzoraka. Po godinama, od 2006. do 2012. godine dijagnostikovano je 192, 160, 233, 181, 104, 43 i 10 pozitivnih slučajeva. U cilju iskorenjivanja besnila na teritoriji Republike Srbije od 2010. godine se sprovodi regionalni projekat oralne vakcinacije lisica protiv besnila. U okviru monitoringa efikasnosti oralne vakcinacije lisica protiv besnila koji je sprovođen krajem 2011. i početkom 2012. godine ispitano je 1385 uzoraka od čega je 11 reagovalo pozitivno. Virus je dominantno prisutan u populaciji lisica. Tokom 2008. i 2009. godine dijagnostikavan je relativno veliki broj besnih mačaka, što ukazuje na prenos virusa iz populacije lisica u populaciju mačaka. Incidencija besnila na teritoriji Srbije se značajno razlikuje od jednog do drugog epizootiološkog područja. Severni delovi zemlje (somborsko i subotičko epizotiološko područje) imaju povoljniju epizootiološku situaciju (samo dva slučaja besnila u proteklih 7 godina). U periodu do 2010. godine na požarevačkom, novosadskom, jagodinskom, pančevačkom, beogradskom i niškom epizootiološkom području se uočava postepeni pad broja pozitivnih slučajeva. Međunarodni projekat oralne vakcinacije lisica protiv besnila koji se sprovodi na teritoriji Republike Srbije i okolnih zemalja uticao je na to da se broj prijavljenih slučajeva besnila u Srbiji drastično smanji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.
T1  - Besnilo - epizootiološka situacija na teritoriji Srbije i zemalja u okruženju od 2006. do 2012. godine
VL  - 67
IS  - 5-6
SP  - 377
EP  - 394
DO  - 10.2298/VETGL1306377M
ER  - 

@article{
author = "Maksimović-Zorić, Jelena and Milićević, Vesna and Veljović, Ljubiša and Petrović, Tamaš and Valčić, Miroslav and Plavšić, Branislav and Vranješ, N.",
year = "2013",
abstract = "Rabies is a disease that occurs both in animals and people, with relatively long period of incubation, intense clinical course and fatal ending. Majority of cases of illnes and death in people are caused by the standard rabies virus. According to books of regulations on suppression and eradiction of rabies from 1988. and 2009., each suspected rabies has to be laboratory confirmed. From 2006. to 2012. within the passive surveilance, 3549 samples of brain tissue were examined for the presence of rabies virus. The virus was confirmed in 923 samples by using the method of direct imunofluorescence. From 2006. to 2012. there were diagnosed 192, 160, 233, 181, 104, 43 and 10 positive cases, respectively. For the purpose of rabies eradiction at the territory of the Republic of Serbia, a regional project of oral vaccination of foxes against rabies has been implemented since 2010.Within monitoring of the effectiveness of oral vaccination against rabies which was carried out at the end of 2011. and at the beginning of 2012., there were examined 1385 samples, out of which 11 reacted positively. The virus was dominantly present in the fox population. During 2008. and 2009., a relatively large number of rabid cats was registered, what indicates a transmission of rabies from the fox to the cat population. The incidence of rabies at the territory of Serbia significantly differs from one epizootic area to another. Northern parts of the country (Sombor and Subotica epizootic regions) have more favorable situation with only two cases of rabies for the last 7 years. In the period until 2010., in Pozarevac, Novi Sad, Jagodina, Pancevo, Belgrade and Nis epizootic region, a gradual decline in number of positive cases was noticed. International project for oral vaccination of foxes against rabies which has been implemented at the territory of the Republic of Serbia and neighboring countries, influenced a significant reduction of registered rabies cases., Besnilo je bolest životinja i ljudi koja se odlikuje relativno dugim inkubacionim periodom i dramatičnim kliničkim tokom, koji se uvek završava smrtnim ishodom. Najveći broj slučajeva oboljenja i smrti ljudi potiče od infekcije klasičnim virusom besnila. Prema pravilnicima koji se bave suzbijanjem i iskorenjivanjem besnila iz 1988. i 2009. godine obavezna je laboratorijska potvrda svake klinički opravdane sumnje na besnilo. Od 2006. do 2012. godine u okviru pasivnog nadzora pregledano je 3549 uzoraka moždanog tkiva na prisustvo virusa besnila. Metodom direktne imunofluorescencije virus je dokazan u 923 uzoraka. Po godinama, od 2006. do 2012. godine dijagnostikovano je 192, 160, 233, 181, 104, 43 i 10 pozitivnih slučajeva. U cilju iskorenjivanja besnila na teritoriji Republike Srbije od 2010. godine se sprovodi regionalni projekat oralne vakcinacije lisica protiv besnila. U okviru monitoringa efikasnosti oralne vakcinacije lisica protiv besnila koji je sprovođen krajem 2011. i početkom 2012. godine ispitano je 1385 uzoraka od čega je 11 reagovalo pozitivno. Virus je dominantno prisutan u populaciji lisica. Tokom 2008. i 2009. godine dijagnostikavan je relativno veliki broj besnih mačaka, što ukazuje na prenos virusa iz populacije lisica u populaciju mačaka. Incidencija besnila na teritoriji Srbije se značajno razlikuje od jednog do drugog epizootiološkog područja. Severni delovi zemlje (somborsko i subotičko epizotiološko područje) imaju povoljniju epizootiološku situaciju (samo dva slučaja besnila u proteklih 7 godina). U periodu do 2010. godine na požarevačkom, novosadskom, jagodinskom, pančevačkom, beogradskom i niškom epizootiološkom području se uočava postepeni pad broja pozitivnih slučajeva. Međunarodni projekat oralne vakcinacije lisica protiv besnila koji se sprovodi na teritoriji Republike Srbije i okolnih zemalja uticao je na to da se broj prijavljenih slučajeva besnila u Srbiji drastično smanji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012., Besnilo - epizootiološka situacija na teritoriji Srbije i zemalja u okruženju od 2006. do 2012. godine",
volume = "67",
number = "5-6",
pages = "377-394",
doi = "10.2298/VETGL1306377M"
}

Maksimović-Zorić, J., Milićević, V., Veljović, L., Petrović, T., Valčić, M., Plavšić, B.,& Vranješ, N.. (2013). Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(5-6), 377-394.
https://doi.org/10.2298/VETGL1306377M

Maksimović-Zorić J, Milićević V, Veljović L, Petrović T, Valčić M, Plavšić B, Vranješ N. Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.. in Veterinarski Glasnik. 2013;67(5-6):377-394.
doi:10.2298/VETGL1306377M .

Maksimović-Zorić, Jelena, Milićević, Vesna, Veljović, Ljubiša, Petrović, Tamaš, Valčić, Miroslav, Plavšić, Branislav, Vranješ, N., "Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012." in Veterinarski Glasnik, 67, no. 5-6 (2013):377-394,
https://doi.org/10.2298/VETGL1306377M . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Veljović, Ljubiša
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/479
AB  - The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera.
AB  - Cilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena njihova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro
T1  - Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro
VL  - 57
IS  - 1
SP  - 3
EP  - 10
DO  - 10.2298/AVB0701003N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Veljović, Ljubiša",
year = "2007",
abstract = "The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera., Cilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena njihova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro, Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro",
volume = "57",
number = "1",
pages = "3-10",
doi = "10.2298/AVB0701003N"
}

Nišavić, J., Milić, N.,& Veljović, L.. (2007). Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(1), 3-10.
https://doi.org/10.2298/AVB0701003N

Nišavić J, Milić N, Veljović L. Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro. in Acta Veterinaria-Beograd. 2007;57(1):3-10.
doi:10.2298/AVB0701003N .

Nišavić, Jakov, Milić, Nenad, Veljović, Ljubiša, "Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro" in Acta Veterinaria-Beograd, 57, no. 1 (2007):3-10,
https://doi.org/10.2298/AVB0701003N . .