TY  - JOUR
AU  - Prošić, Isidora
AU  - Milčić Matić, Natalija
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Aksentijević, Ksenija
AU  - Ilić, Milica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Gajdov, Vladimir
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3763
AB  - The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study
VL  - 74
IS  - 1
SP  - 117
EP  - 132
DO  - 10.2478/acve-2024-0009
DO  - 10.2478/acve-2024-0009
ER  - 

@article{
author = "Prošić, Isidora and Milčić Matić, Natalija and Milić, Nenad and Radalj, Andrea and Aksentijević, Ksenija and Ilić, Milica and Nišavić, Jakov and Radojičić, Marina and Gajdov, Vladimir and Krnjaić, Dejan",
year = "2024",
abstract = "The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study",
volume = "74",
number = "1",
pages = "117-132",
doi = "10.2478/acve-2024-0009, 10.2478/acve-2024-0009"
}

Prošić, I., Milčić Matić, N., Milić, N., Radalj, A., Aksentijević, K., Ilić, M., Nišavić, J., Radojičić, M., Gajdov, V.,& Krnjaić, D.. (2024). Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 117-132.
https://doi.org/10.2478/acve-2024-0009

Prošić I, Milčić Matić N, Milić N, Radalj A, Aksentijević K, Ilić M, Nišavić J, Radojičić M, Gajdov V, Krnjaić D. Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria. 2024;74(1):117-132.
doi:10.2478/acve-2024-0009 .

Prošić, Isidora, Milčić Matić, Natalija, Milić, Nenad, Radalj, Andrea, Aksentijević, Ksenija, Ilić, Milica, Nišavić, Jakov, Radojičić, Marina, Gajdov, Vladimir, Krnjaić, Dejan, "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study" in Acta Veterinaria, 74, no. 1 (2024):117-132,
https://doi.org/10.2478/acve-2024-0009 . .

TY  - CONF
AU  - Aksentijević, Ksenija
AU  - Marković, Maja
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3718
AB  - Akvarijumske ribe su jedna od najvećih grupa kućnih ljubimaca
na svetu. Sve je veća potreba za uključivanjem veterinara u negu i lečenje
ukrasnih i akavrijumskih riba, bilo da se radi o klijentima u privatnim
kućama, maloprodajnim i veleprodajnim objektima.
Nema logike da se sadašnji i budući veterinari tako olako odreknu
velikog dela svojih pacijenata. Prema podacima iz različitih zemalja
barem 50% vlasnika pasa i mačaka kod kuće ima i akvarijum koji je
prepušten na milost i nemilost raznim akvarističkim forumima, prodavcima
i odgajivačima riba i drugim osobama koje nisu veterinari.
Ribe jesu sa jedne strane znatno drugačiji pacijenti u odnosu na
uobičajene pacijente u maloj praksi, ali sa pravilnim razumevanjem
ekosistema u kome žive, pažnjom prema posebnim i opštim potrebama
pojedinih vrsta moguće im je i sa minimalnom dodatnom obukom
pomoći.
Veterinarska nega riba kućnih ljubimaca, izložbenih riba i vrednih
matičnih jata uključuje laboratorijske procedure (analize krvi, mikrobiologiju,
prazitologiju, histopatologiju), radiologiju, ultrazvučnu dijagnostiku,
kao i terapiju. Napredak u hirurgiji poboljšava i dijagnostiku i tretman
bolesti riba.
Većina potrebne opreme za dijagnostiku bolesti riba već postoji
u prosečnoj ambulanti za male životinje a oprema koja nedostaje nije
previše skupa.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Akvarijumske ribe pacijenti male prakse – osnovna oprema i veštine
SP  - 83
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3718
ER  - 

@conference{
author = "Aksentijević, Ksenija and Marković, Maja",
year = "2024",
abstract = "Akvarijumske ribe su jedna od najvećih grupa kućnih ljubimaca
na svetu. Sve je veća potreba za uključivanjem veterinara u negu i lečenje
ukrasnih i akavrijumskih riba, bilo da se radi o klijentima u privatnim
kućama, maloprodajnim i veleprodajnim objektima.
Nema logike da se sadašnji i budući veterinari tako olako odreknu
velikog dela svojih pacijenata. Prema podacima iz različitih zemalja
barem 50% vlasnika pasa i mačaka kod kuće ima i akvarijum koji je
prepušten na milost i nemilost raznim akvarističkim forumima, prodavcima
i odgajivačima riba i drugim osobama koje nisu veterinari.
Ribe jesu sa jedne strane znatno drugačiji pacijenti u odnosu na
uobičajene pacijente u maloj praksi, ali sa pravilnim razumevanjem
ekosistema u kome žive, pažnjom prema posebnim i opštim potrebama
pojedinih vrsta moguće im je i sa minimalnom dodatnom obukom
pomoći.
Veterinarska nega riba kućnih ljubimaca, izložbenih riba i vrednih
matičnih jata uključuje laboratorijske procedure (analize krvi, mikrobiologiju,
prazitologiju, histopatologiju), radiologiju, ultrazvučnu dijagnostiku,
kao i terapiju. Napredak u hirurgiji poboljšava i dijagnostiku i tretman
bolesti riba.
Većina potrebne opreme za dijagnostiku bolesti riba već postoji
u prosečnoj ambulanti za male životinje a oprema koja nedostaje nije
previše skupa.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Akvarijumske ribe pacijenti male prakse – osnovna oprema i veštine",
pages = "83-98",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3718"
}

Aksentijević, K.,& Marković, M.. (2024). Akvarijumske ribe pacijenti male prakse – osnovna oprema i veštine. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 83-98.
https://hdl.handle.net/21.15107/rcub_veterinar_3718

Aksentijević K, Marković M. Akvarijumske ribe pacijenti male prakse – osnovna oprema i veštine. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:83-98.
https://hdl.handle.net/21.15107/rcub_veterinar_3718 .

Aksentijević, Ksenija, Marković, Maja, "Akvarijumske ribe pacijenti male prakse – osnovna oprema i veštine" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):83-98,
https://hdl.handle.net/21.15107/rcub_veterinar_3718 .

TY  - JOUR
AU  - Baltić, Branislav
AU  - Aksentijević, Ksenija
AU  - Bogunović, Danica
AU  - Starčević, Marija
AU  - Mitrović, Radmila
AU  - Mrdović, Boris
AU  - Janjić, Jelena
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3767
AB  - In the Serbian market, fish is available from aquaculture (such as carp and trout) and from
fishing (commercial and recreational catch). In the past ten years, from 2012 to 2021, there
has been a decrease in the production of carp due to reduced farming areas, but trout production
has increased. On average, during this period, aquaculture production yielded approximately
5,491 tons of carp and 2,977 tons of trout, while the fish catch averaged around
2,979 tons. The yield per hectare in carp ponds was 800 kg, while in trout ponds, it was
20 kg per square meter. As the demand for fish exceeds the domestic supply, the market is
supplemented with imported fish.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Investigation of the volume of fish production and catch in Serbia from 2012 to 2021
VL  - 64
IS  - 2
SP  - 329
EP  - 333
DO  - 10.18485/meattech.2023.64.2.62
ER  - 

@article{
author = "Baltić, Branislav and Aksentijević, Ksenija and Bogunović, Danica and Starčević, Marija and Mitrović, Radmila and Mrdović, Boris and Janjić, Jelena",
year = "2023",
abstract = "In the Serbian market, fish is available from aquaculture (such as carp and trout) and from
fishing (commercial and recreational catch). In the past ten years, from 2012 to 2021, there
has been a decrease in the production of carp due to reduced farming areas, but trout production
has increased. On average, during this period, aquaculture production yielded approximately
5,491 tons of carp and 2,977 tons of trout, while the fish catch averaged around
2,979 tons. The yield per hectare in carp ponds was 800 kg, while in trout ponds, it was
20 kg per square meter. As the demand for fish exceeds the domestic supply, the market is
supplemented with imported fish.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Investigation of the volume of fish production and catch in Serbia from 2012 to 2021",
volume = "64",
number = "2",
pages = "329-333",
doi = "10.18485/meattech.2023.64.2.62"
}

Baltić, B., Aksentijević, K., Bogunović, D., Starčević, M., Mitrović, R., Mrdović, B.,& Janjić, J.. (2023). Investigation of the volume of fish production and catch in Serbia from 2012 to 2021. in Meat Technology
Institute of Meat Hygiene and Technology., 64(2), 329-333.
https://doi.org/10.18485/meattech.2023.64.2.62

Baltić B, Aksentijević K, Bogunović D, Starčević M, Mitrović R, Mrdović B, Janjić J. Investigation of the volume of fish production and catch in Serbia from 2012 to 2021. in Meat Technology. 2023;64(2):329-333.
doi:10.18485/meattech.2023.64.2.62 .

Baltić, Branislav, Aksentijević, Ksenija, Bogunović, Danica, Starčević, Marija, Mitrović, Radmila, Mrdović, Boris, Janjić, Jelena, "Investigation of the volume of fish production and catch in Serbia from 2012 to 2021" in Meat Technology, 64, no. 2 (2023):329-333,
https://doi.org/10.18485/meattech.2023.64.2.62 . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Radalj, Andrea
AU  - Marković, Maja
AU  - Lazarević, Miodrag
AU  - Palić, Dušan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2955
AB  - Stress inevitably occurs during any fish handling and manipulation in culturing, research,
or clinical examination situations that require capture and removal of fish from water.
Different stress factors can affect the changes in the relative numbers and function of
cells of the fish immune system. Catching, transportation and over-crowding caused
stress-induced changes in the total number of leukocytes and thrombocytes, as well
as changes in the leukocyte formula in Prussian carp (Carassius gibelio, Bloch, 1782)
presented here. Cytochemical characterization of leukocyte cell types was performed by
applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) and Sudan Black B (SBB)
staining of Prussian carp blood smears. Cytochemical characterization is a rapid and
efficient method for white leukocyte differentiation and insight in their functional status.
Comparison and analysis in Prussian carp hematological parameters from fish with
and without exposure to stressful conditions such as capture, manipulation, transport
and holding, revealed significant differences between stressed and non-stressed fish.
Significant reduction in the total number of thrombocytes and lymphocytes and
the increase in total neutrophil count were observed in stressed animals. However,
differences in total leukocyte number and the number of monocytes were not observed.
Deviations from the estimated reference intervals for Prussian carp hematological
parameters clearly indicated the presence/absence of a stress reaction and to some
extent its intensity. Estimated reference intervals and characterization of morphological
and cytochemical appearance of blood cells form a solid basis for further research of
the cellular immune function and hematology changes in Prussian carp.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Acta Veterinaria
T1  - Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern
VL  - 73
IS  - 2
SP  - 179
EP  - 194
DO  - 10.2478/acve-2023-0014
ER  - 

@article{
author = "Aksentijević, Ksenija and Radalj, Andrea and Marković, Maja and Lazarević, Miodrag and Palić, Dušan",
year = "2023",
abstract = "Stress inevitably occurs during any fish handling and manipulation in culturing, research,
or clinical examination situations that require capture and removal of fish from water.
Different stress factors can affect the changes in the relative numbers and function of
cells of the fish immune system. Catching, transportation and over-crowding caused
stress-induced changes in the total number of leukocytes and thrombocytes, as well
as changes in the leukocyte formula in Prussian carp (Carassius gibelio, Bloch, 1782)
presented here. Cytochemical characterization of leukocyte cell types was performed by
applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) and Sudan Black B (SBB)
staining of Prussian carp blood smears. Cytochemical characterization is a rapid and
efficient method for white leukocyte differentiation and insight in their functional status.
Comparison and analysis in Prussian carp hematological parameters from fish with
and without exposure to stressful conditions such as capture, manipulation, transport
and holding, revealed significant differences between stressed and non-stressed fish.
Significant reduction in the total number of thrombocytes and lymphocytes and
the increase in total neutrophil count were observed in stressed animals. However,
differences in total leukocyte number and the number of monocytes were not observed.
Deviations from the estimated reference intervals for Prussian carp hematological
parameters clearly indicated the presence/absence of a stress reaction and to some
extent its intensity. Estimated reference intervals and characterization of morphological
and cytochemical appearance of blood cells form a solid basis for further research of
the cellular immune function and hematology changes in Prussian carp.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Acta Veterinaria",
title = "Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern",
volume = "73",
number = "2",
pages = "179-194",
doi = "10.2478/acve-2023-0014"
}

Aksentijević, K., Radalj, A., Marković, M., Lazarević, M.,& Palić, D.. (2023). Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern. in Acta Veterinaria
University of Belgrade, Faculty of Veterinary Medicine., 73(2), 179-194.
https://doi.org/10.2478/acve-2023-0014

Aksentijević K, Radalj A, Marković M, Lazarević M, Palić D. Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern. in Acta Veterinaria. 2023;73(2):179-194.
doi:10.2478/acve-2023-0014 .

Aksentijević, Ksenija, Radalj, Andrea, Marković, Maja, Lazarević, Miodrag, Palić, Dušan, "Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern" in Acta Veterinaria, 73, no. 2 (2023):179-194,
https://doi.org/10.2478/acve-2023-0014 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Zuber-Bogdanovic, Ivana
AU  - Grković, Nevena
AU  - Aksentijević, Ksenija
AU  - Nikolic, Marko
AU  - Pavicevic, Zorica
AU  - Lausevic, Dejan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2707
AB  - Shellfish for human consumption is produced in excess of 15 million tons per year, accounting for roughly 14% of  global marine aquaculture, with China being the largest producer (89% of world production). However, in the previous two decades, shellfish output in the European Union has been dropping. This review provides information about shellfish aquaculture in Montenegro, in Boka Kotorska Bay, where most farms belong to the group of small farms. Shellfish production in this bay is constantly increasing. Thus, with minor deviations, the production of shellfish increased from 156 tonnes, as was produced in 2012, to 246 tonnes produced in 2020. However, despite the constant growth of  production, statistical data indicate that every year a large amount of shellfish is imported, and there is a need for greater production in the country. The shellfish production capacity in Montenegro is still lower than expected, despite the  great  potential  for  development.  Aquaculture’s  development  potential  is  in  the  areas of increasing production, modernising existing farms, automating production, strengthening  the  competitiveness  and  efficiency  of  the  sector,  introducing  new species, better linking aquaculture with tourism and processing, marine spatial planning, promoting aquaculture products and branding products.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Sustainability of shellfish aquaculture in Montenegro-perspectives
T1  - Održiva proizvodnja školjki u Crnoj Gori – perspektive
VL  - 76
IS  - 2
IS  - 125
EP  - 135
DO  - 10.2298/VETGL220315010D
ER  - 

@article{
author = "Dimitrijević, Mirjana and Zuber-Bogdanovic, Ivana and Grković, Nevena and Aksentijević, Ksenija and Nikolic, Marko and Pavicevic, Zorica and Lausevic, Dejan",
year = "2022",
abstract = "Shellfish for human consumption is produced in excess of 15 million tons per year, accounting for roughly 14% of  global marine aquaculture, with China being the largest producer (89% of world production). However, in the previous two decades, shellfish output in the European Union has been dropping. This review provides information about shellfish aquaculture in Montenegro, in Boka Kotorska Bay, where most farms belong to the group of small farms. Shellfish production in this bay is constantly increasing. Thus, with minor deviations, the production of shellfish increased from 156 tonnes, as was produced in 2012, to 246 tonnes produced in 2020. However, despite the constant growth of  production, statistical data indicate that every year a large amount of shellfish is imported, and there is a need for greater production in the country. The shellfish production capacity in Montenegro is still lower than expected, despite the  great  potential  for  development.  Aquaculture’s  development  potential  is  in  the  areas of increasing production, modernising existing farms, automating production, strengthening  the  competitiveness  and  efficiency  of  the  sector,  introducing  new species, better linking aquaculture with tourism and processing, marine spatial planning, promoting aquaculture products and branding products.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Sustainability of shellfish aquaculture in Montenegro-perspectives, Održiva proizvodnja školjki u Crnoj Gori – perspektive",
volume = "76",
number = "2, 125",
pages = "135",
doi = "10.2298/VETGL220315010D"
}

Dimitrijević, M., Zuber-Bogdanovic, I., Grković, N., Aksentijević, K., Nikolic, M., Pavicevic, Z.,& Lausevic, D.. (2022). Sustainability of shellfish aquaculture in Montenegro-perspectives. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 76(2).
https://doi.org/10.2298/VETGL220315010D

Dimitrijević M, Zuber-Bogdanovic I, Grković N, Aksentijević K, Nikolic M, Pavicevic Z, Lausevic D. Sustainability of shellfish aquaculture in Montenegro-perspectives. in Veterinarski Glasnik. 2022;76(2):null-135.
doi:10.2298/VETGL220315010D .

Dimitrijević, Mirjana, Zuber-Bogdanovic, Ivana, Grković, Nevena, Aksentijević, Ksenija, Nikolic, Marko, Pavicevic, Zorica, Lausevic, Dejan, "Sustainability of shellfish aquaculture in Montenegro-perspectives" in Veterinarski Glasnik, 76, no. 2 (2022),
https://doi.org/10.2298/VETGL220315010D . .

TY  - JOUR
AU  - Palić, Dušan
AU  - Aksentijević, Ksenija
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2710
AB  - New technological progress and increased demands for fish as a source of animal protein are driving significant growth of aquaculture production. Intensification of production increases the severity and frequency of infectious disease outbreaks, and so requires significant effort to prevent and control disease. Because of the global crisis of bacterial resistance to antibiotics, the use of antibiotics in aquaculture is increasingly subjected to strict control and regulatory measures, leading to potential misuse. The lack of availability of approved veterinary medical products for use in aquaculture, combined with the risk of drug resistance development and antibiotic residues in fish flesh or water, support the development of preventive actions, including vaccines. However, the diversity of species and aquaculture production methods, including epidemiological units and their links, results in economic challenges for commercial vaccine development and authorization. As a possible response to the increasing demand for less antibiotic use in fish farms, and to the expenses associated with novel veterinary product development, there is a need for increased use of safe and effective autogenous vaccines in aquaculture. Regulatory processes for autogenous vaccine production, approval and application should recognize the specificities of epidemiological units and their links in aquatic animal production facilities. The joint efforts of regulatory authorities, producers, and veterinary services to follow veterinary biosecurity principles, including risk analysis, surveillance, and selection/prioritization of pathogens, are essential to provide maximum safety and efficacy of autogenous vaccines as disease prevention and control tools within larger areas, such as compartments and zones, and allow for reductions in antibiotic use.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Autogenous vaccines in aquaculture: tool to combat resistance of bacteria to antibiotics?
VL  - 76
VL  - 2
SP  - 91
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2710
ER  - 

@article{
author = "Palić, Dušan and Aksentijević, Ksenija",
year = "2022",
abstract = "New technological progress and increased demands for fish as a source of animal protein are driving significant growth of aquaculture production. Intensification of production increases the severity and frequency of infectious disease outbreaks, and so requires significant effort to prevent and control disease. Because of the global crisis of bacterial resistance to antibiotics, the use of antibiotics in aquaculture is increasingly subjected to strict control and regulatory measures, leading to potential misuse. The lack of availability of approved veterinary medical products for use in aquaculture, combined with the risk of drug resistance development and antibiotic residues in fish flesh or water, support the development of preventive actions, including vaccines. However, the diversity of species and aquaculture production methods, including epidemiological units and their links, results in economic challenges for commercial vaccine development and authorization. As a possible response to the increasing demand for less antibiotic use in fish farms, and to the expenses associated with novel veterinary product development, there is a need for increased use of safe and effective autogenous vaccines in aquaculture. Regulatory processes for autogenous vaccine production, approval and application should recognize the specificities of epidemiological units and their links in aquatic animal production facilities. The joint efforts of regulatory authorities, producers, and veterinary services to follow veterinary biosecurity principles, including risk analysis, surveillance, and selection/prioritization of pathogens, are essential to provide maximum safety and efficacy of autogenous vaccines as disease prevention and control tools within larger areas, such as compartments and zones, and allow for reductions in antibiotic use.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Autogenous vaccines in aquaculture: tool to combat resistance of bacteria to antibiotics?",
volume = "76, 2",
pages = "91-102",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2710"
}

Palić, D.,& Aksentijević, K.. (2022). Autogenous vaccines in aquaculture: tool to combat resistance of bacteria to antibiotics?. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 76, 91-102.
https://hdl.handle.net/21.15107/rcub_veterinar_2710

Palić D, Aksentijević K. Autogenous vaccines in aquaculture: tool to combat resistance of bacteria to antibiotics?. in Veterinarski Glasnik. 2022;76:91-102.
https://hdl.handle.net/21.15107/rcub_veterinar_2710 .

Palić, Dušan, Aksentijević, Ksenija, "Autogenous vaccines in aquaculture: tool to combat resistance of bacteria to antibiotics?" in Veterinarski Glasnik, 76 (2022):91-102,
https://hdl.handle.net/21.15107/rcub_veterinar_2710 .

TY  - GEN
AU  - Aksentijević, Ksenija
AU  - Marković, Maja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3166
AB  - Svuda u svetu se beleži izuzetan rast proizvodnje u akvakulturi. Ona čini 44 procenta
ukupne globalne proizvodnje riba i ovaj porast se postiže uprkos suočavanju sa
mnogim izazovima proizvodnje u vodenoj sredini. Važan ograničavajući faktor u proizvodnji
su svakako infektivne bolesti koje godišnje dovode do milionskih gubitaka. U cilju
smanjenja uticaja infektivnih bolesti na proizvodne rezultate neophodno je redovno
i u kontinuitetu, primenjivati naučno dokazane i preporučene metode. Ovaj rad ima
za cilj da ukaže na neke od najboljih pristupa prevenciji i kontroli zaraznih bolesti u
akvakulturi. Među efikasnim strategijama prevencije i kontrole, jedan od ključnih načina
je vakcinacija. Vrste vakcina za upotrebu u akvakulturi uključuju: inaktivisane (mrtve),
atenuirane (žive/oslabljene), vakcine na bazi dezoksiribonukleinske kiseline (DNK
vakcine), rekombinantne vektorske vakcine i subjedinične vakcine. Načini njihove aplikacije
uključuju: oralne, injekcione i imerzione metode. Antibiotici se upotrebljavaju u
akvakulturi uprkos potencijalnom riziku od razvoja i širenja rezistencije među bakterijama.
Sve više su zastupljene strategije za biološku i hemijsku kontrolu bolesti kao što
su upotreba probiotika, prebiotika i različitih ekstrakta biljaka. Biosigurnosne mere u
akvakulturi štite proizvodnju od određenih agenasa koji izazivaju bolesti, a podrazumevaju
stroge karantinske mere, dezinfekciju ikre, kontrolu ulaz/izlaz, tretiranje vode,
čistu hranu i odlaganje uginulih jedinki. Kao zaključak i savet, navodimo da je umesto
tretiranja svakog slučaja izbijanja bolesti daleko bolje primenjivati preventivni pristup
pre pojave zaraze.
PB  - Beograd : Srpsko veterinarsko društvo
T2  - 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021
T1  - Održavanje zdravlja riba u akvakulturi: epidemiološki pristup prevenciji i kontroli infektivnih bolesti
SP  - 234
EP  - 244
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3166
ER  - 

@misc{
author = "Aksentijević, Ksenija and Marković, Maja",
year = "2021",
abstract = "Svuda u svetu se beleži izuzetan rast proizvodnje u akvakulturi. Ona čini 44 procenta
ukupne globalne proizvodnje riba i ovaj porast se postiže uprkos suočavanju sa
mnogim izazovima proizvodnje u vodenoj sredini. Važan ograničavajući faktor u proizvodnji
su svakako infektivne bolesti koje godišnje dovode do milionskih gubitaka. U cilju
smanjenja uticaja infektivnih bolesti na proizvodne rezultate neophodno je redovno
i u kontinuitetu, primenjivati naučno dokazane i preporučene metode. Ovaj rad ima
za cilj da ukaže na neke od najboljih pristupa prevenciji i kontroli zaraznih bolesti u
akvakulturi. Među efikasnim strategijama prevencije i kontrole, jedan od ključnih načina
je vakcinacija. Vrste vakcina za upotrebu u akvakulturi uključuju: inaktivisane (mrtve),
atenuirane (žive/oslabljene), vakcine na bazi dezoksiribonukleinske kiseline (DNK
vakcine), rekombinantne vektorske vakcine i subjedinične vakcine. Načini njihove aplikacije
uključuju: oralne, injekcione i imerzione metode. Antibiotici se upotrebljavaju u
akvakulturi uprkos potencijalnom riziku od razvoja i širenja rezistencije među bakterijama.
Sve više su zastupljene strategije za biološku i hemijsku kontrolu bolesti kao što
su upotreba probiotika, prebiotika i različitih ekstrakta biljaka. Biosigurnosne mere u
akvakulturi štite proizvodnju od određenih agenasa koji izazivaju bolesti, a podrazumevaju
stroge karantinske mere, dezinfekciju ikre, kontrolu ulaz/izlaz, tretiranje vode,
čistu hranu i odlaganje uginulih jedinki. Kao zaključak i savet, navodimo da je umesto
tretiranja svakog slučaja izbijanja bolesti daleko bolje primenjivati preventivni pristup
pre pojave zaraze.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021",
title = "Održavanje zdravlja riba u akvakulturi: epidemiološki pristup prevenciji i kontroli infektivnih bolesti",
pages = "234-244",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3166"
}

Aksentijević, K.,& Marković, M.. (2021). Održavanje zdravlja riba u akvakulturi: epidemiološki pristup prevenciji i kontroli infektivnih bolesti. in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021
Beograd : Srpsko veterinarsko društvo., 234-244.
https://hdl.handle.net/21.15107/rcub_veterinar_3166

Aksentijević K, Marković M. Održavanje zdravlja riba u akvakulturi: epidemiološki pristup prevenciji i kontroli infektivnih bolesti. in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021. 2021;:234-244.
https://hdl.handle.net/21.15107/rcub_veterinar_3166 .

Aksentijević, Ksenija, Marković, Maja, "Održavanje zdravlja riba u akvakulturi: epidemiološki pristup prevenciji i kontroli infektivnih bolesti" in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021 (2021):234-244,
https://hdl.handle.net/21.15107/rcub_veterinar_3166 .

TY  - CONF
AU  - Aksentijević, Ksenija
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3168
AB  - Akvakultura je grana poljoprivrede koja ubrzano raste i koja trenutno obezbeđuje
više od polovine ukupne količine riba koje se širom sveta koriste za ishranu ljudi.
Kako proizvodnja raste, tako raste i količina antibiotika koji se koriste za sprečavanje
i lečenje bakterijskih infekcija riba. Prekomerna i pogrešna upotreba ili zloupotreba
antibiotika i dezinfekcionih sredstava u akvakulturi može dovesti do pojave otpornosti
bakterija sa najvažnijom medicinskom posledicom gubitka efikasnosti antibiotika. Takođe
postoji i mogućnost, da povećan broj bakterija otpornih na antibiotike ili gena koji
kodiraju rezistenciju, ima negativan uticaj na upotrebu antimikrobnih sredstava za
kontrolu bolesti ljudi i drugih kopnenih životinja. Koraci koje treba preduzeti za sprečavanje
i smanjenje upotrebe antibiotika u akvakulturi su: upotreba vakcina kad god
je to moguće; uvođenje specifičnih mera biološke sigurnosti; razvoj posebnih programa
praćenja radi sprečavanja ili smanjenja mogućih izbijanja bolesti; razvoj proizvodnih
sistema koji su optimalni u pogledu kvaliteta vode i nivoa kiseonika i koji mogu garantovati
dobrobit uzgajanih životinja. Duga bitka protiv antimikrobne rezistencije mogla
bi se prevazići iskrenom primenom pristupa „Jedno zdravlje“.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021
T1  - Pojava antimikrobne rezistencije u akvakulturi – šta do sada znamo i koji su sledeći koraci?
SP  - 258
EP  - 263
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3168
ER  - 

@conference{
author = "Aksentijević, Ksenija",
year = "2021",
abstract = "Akvakultura je grana poljoprivrede koja ubrzano raste i koja trenutno obezbeđuje
više od polovine ukupne količine riba koje se širom sveta koriste za ishranu ljudi.
Kako proizvodnja raste, tako raste i količina antibiotika koji se koriste za sprečavanje
i lečenje bakterijskih infekcija riba. Prekomerna i pogrešna upotreba ili zloupotreba
antibiotika i dezinfekcionih sredstava u akvakulturi može dovesti do pojave otpornosti
bakterija sa najvažnijom medicinskom posledicom gubitka efikasnosti antibiotika. Takođe
postoji i mogućnost, da povećan broj bakterija otpornih na antibiotike ili gena koji
kodiraju rezistenciju, ima negativan uticaj na upotrebu antimikrobnih sredstava za
kontrolu bolesti ljudi i drugih kopnenih životinja. Koraci koje treba preduzeti za sprečavanje
i smanjenje upotrebe antibiotika u akvakulturi su: upotreba vakcina kad god
je to moguće; uvođenje specifičnih mera biološke sigurnosti; razvoj posebnih programa
praćenja radi sprečavanja ili smanjenja mogućih izbijanja bolesti; razvoj proizvodnih
sistema koji su optimalni u pogledu kvaliteta vode i nivoa kiseonika i koji mogu garantovati
dobrobit uzgajanih životinja. Duga bitka protiv antimikrobne rezistencije mogla
bi se prevazići iskrenom primenom pristupa „Jedno zdravlje“.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021",
title = "Pojava antimikrobne rezistencije u akvakulturi – šta do sada znamo i koji su sledeći koraci?",
pages = "258-263",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3168"
}

Aksentijević, K.. (2021). Pojava antimikrobne rezistencije u akvakulturi – šta do sada znamo i koji su sledeći koraci?. in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021
Beograd : Srpsko veterinarsko društvo., 258-263.
https://hdl.handle.net/21.15107/rcub_veterinar_3168

Aksentijević K. Pojava antimikrobne rezistencije u akvakulturi – šta do sada znamo i koji su sledeći koraci?. in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021. 2021;:258-263.
https://hdl.handle.net/21.15107/rcub_veterinar_3168 .

Aksentijević, Ksenija, "Pojava antimikrobne rezistencije u akvakulturi – šta do sada znamo i koji su sledeći koraci?" in 32. Savetovanje veterinara Srbije,Zlatibor, 9 – 12. septembar 2021 (2021):258-263,
https://hdl.handle.net/21.15107/rcub_veterinar_3168 .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Tadić, Vanja
AU  - Korzeniowska, Malgorzata
AU  - Nišavić, Jakov
AU  - Aksentijević, Ksenija
AU  - Kuzmanovic, Jelena
AU  - Žižović, Irena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1855
AB  - Supercritical fluid extraction as an environmentally friendly technology was applied to isolate biologically active extracts from celery and parsley fruits for potential applications in the food industry. The extractions were performed under mild temperature conditions of 39.85 °C and at pressures of 10 and 30 MPa. The extracts were analyzed regarding their chemical composition, antibacterial activity, and cytotoxic effect. Sedanolide was the dominant component of the celery fruit extracts, comprising more than 70% of the obtained fraction, while the content of apiole in the parsley fruit SC CO2 extracts exceeded 85%. The celery fruit extracts showed strong and moderately strong antibacterial activity against tested Staphylococcus aureus, Bacillus (B.) cereus, B. subtilis, B. circulans, Listeria (L.) greyi, L. seeligeri and L. welshimeri, with minimal inhibitory concentration (MIC) values between 160 and 640 µg/mL, and weak activity against the selected Salmonella isolates with a MIC of 2560 µg/mL. The parsley extract obtained at 10 MPa showed strong and moderately strong antibacterial effects against Bacillus strains with obtained MICs of 160-640 µg/mL, and weak activity against Staphylococcus, Listeria, and Salmonella with a MIC of 2560 µg/mL. Cytotoxicity investigation showed that the extracts with proven antibacterial activity had no cytotoxic effect on rabbit kidney cells at concentrations of up to 640 µg/mL.
PB  - MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity
VL  - 25
IS  - 14
DO  - 10.3390/molecules25143163
ER  - 

@article{
author = "Mišić, Dušan and Tadić, Vanja and Korzeniowska, Malgorzata and Nišavić, Jakov and Aksentijević, Ksenija and Kuzmanovic, Jelena and Žižović, Irena",
year = "2020",
abstract = "Supercritical fluid extraction as an environmentally friendly technology was applied to isolate biologically active extracts from celery and parsley fruits for potential applications in the food industry. The extractions were performed under mild temperature conditions of 39.85 °C and at pressures of 10 and 30 MPa. The extracts were analyzed regarding their chemical composition, antibacterial activity, and cytotoxic effect. Sedanolide was the dominant component of the celery fruit extracts, comprising more than 70% of the obtained fraction, while the content of apiole in the parsley fruit SC CO2 extracts exceeded 85%. The celery fruit extracts showed strong and moderately strong antibacterial activity against tested Staphylococcus aureus, Bacillus (B.) cereus, B. subtilis, B. circulans, Listeria (L.) greyi, L. seeligeri and L. welshimeri, with minimal inhibitory concentration (MIC) values between 160 and 640 µg/mL, and weak activity against the selected Salmonella isolates with a MIC of 2560 µg/mL. The parsley extract obtained at 10 MPa showed strong and moderately strong antibacterial effects against Bacillus strains with obtained MICs of 160-640 µg/mL, and weak activity against Staphylococcus, Listeria, and Salmonella with a MIC of 2560 µg/mL. Cytotoxicity investigation showed that the extracts with proven antibacterial activity had no cytotoxic effect on rabbit kidney cells at concentrations of up to 640 µg/mL.",
publisher = "MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity",
volume = "25",
number = "14",
doi = "10.3390/molecules25143163"
}

Mišić, D., Tadić, V., Korzeniowska, M., Nišavić, J., Aksentijević, K., Kuzmanovic, J.,& Žižović, I.. (2020). Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity. in Molecules (Basel, Switzerland)
MDPI., 25(14).
https://doi.org/10.3390/molecules25143163

Mišić D, Tadić V, Korzeniowska M, Nišavić J, Aksentijević K, Kuzmanovic J, Žižović I. Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity. in Molecules (Basel, Switzerland). 2020;25(14).
doi:10.3390/molecules25143163 .

Mišić, Dušan, Tadić, Vanja, Korzeniowska, Malgorzata, Nišavić, Jakov, Aksentijević, Ksenija, Kuzmanovic, Jelena, Žižović, Irena, "Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity" in Molecules (Basel, Switzerland), 25, no. 14 (2020),
https://doi.org/10.3390/molecules25143163 . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Aksentijević, Ksenija
AU  - Tambur, Zoran
AU  - Rakonjac, Bojan
AU  - Kovačević, Ivana
AU  - Spergser, Joachim
AU  - Loncarić, Igor
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1758
AB  - The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
VL  - 8
IS  - 1
SP  - 26
DO  - 10.3390/antibiotics8010026
ER  - 

@article{
author = "Ašanin, Jelena and Mišić, Dušan and Aksentijević, Ksenija and Tambur, Zoran and Rakonjac, Bojan and Kovačević, Ivana and Spergser, Joachim and Loncarić, Igor",
year = "2019",
abstract = "The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia",
volume = "8",
number = "1",
pages = "26",
doi = "10.3390/antibiotics8010026"
}

Ašanin, J., Mišić, D., Aksentijević, K., Tambur, Z., Rakonjac, B., Kovačević, I., Spergser, J.,& Loncarić, I.. (2019). Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel
MDPI, Basel., 8(1), 26.
https://doi.org/10.3390/antibiotics8010026

Ašanin J, Mišić D, Aksentijević K, Tambur Z, Rakonjac B, Kovačević I, Spergser J, Loncarić I. Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel. 2019;8(1):26.
doi:10.3390/antibiotics8010026 .

Ašanin, Jelena, Mišić, Dušan, Aksentijević, Ksenija, Tambur, Zoran, Rakonjac, Bojan, Kovačević, Ivana, Spergser, Joachim, Loncarić, Igor, "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia" in Antibiotics-Basel, 8, no. 1 (2019):26,
https://doi.org/10.3390/antibiotics8010026 . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Nišavić, Jakov
AU  - Marković, Maja
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1441
AB  - Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL.
AB  - Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibiotic resistance in bacteria isolated from fish in Serbia
T1  - Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije
VL  - 71
IS  - 1
SP  - 24
EP  - 34
DO  - 10.2298/VETGL170329005A
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan",
year = "2017",
abstract = "Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL., Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibiotic resistance in bacteria isolated from fish in Serbia, Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije",
volume = "71",
number = "1",
pages = "24-34",
doi = "10.2298/VETGL170329005A"
}

Aksentijević, K., Ašanin, J., Nišavić, J., Marković, M., Milanov, D.,& Mišić, D.. (2017). Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 24-34.
https://doi.org/10.2298/VETGL170329005A

Aksentijević K, Ašanin J, Nišavić J, Marković M, Milanov D, Mišić D. Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik. 2017;71(1):24-34.
doi:10.2298/VETGL170329005A .

Aksentijević, Ksenija, Ašanin, Jelena, Nišavić, Jakov, Marković, Maja, Milanov, Dubravka, Mišić, Dušan, "Antibiotic resistance in bacteria isolated from fish in Serbia" in Veterinarski Glasnik, 71, no. 1 (2017):24-34,
https://doi.org/10.2298/VETGL170329005A . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1488
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
SP  - 8762649
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
pages = "8762649",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 8762649.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;:8762649.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science (2017):8762649,
https://doi.org/10.1155/2017/8762649 . .

TY  - THES
AU  - Aksentijević, Ksenija
PY  - 2016
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48634895
UR  - http://nardus.mpn.gov.rs/123456789/7560
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/38
AB  - During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL.
AB  - U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments
T1  - Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7560
ER  - 

@phdthesis{
author = "Aksentijević, Ksenija",
year = "2016",
abstract = "During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL., U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments, Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7560"
}

Aksentijević, K.. (2016). Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_7560

Aksentijević K. Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

Aksentijević, Ksenija, "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

TY  - JOUR
AU  - Marković, Maja
AU  - Radojičić, Marina
AU  - Zdravković, Nemanja
AU  - Lazić, Marko
AU  - Aksentijević, Ksenija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1341
AB  - Although commensals in digestive tract of a large number of fish species, diplomonads represent very significant opportunistic pathogens. For so far unknown reasons, they can proliferate uncontrollably and thus cause changes in the skin and internal organs in aquarium fish. The problem is confusion over nomenclature of the two most important genera: Spironucleus i Hexamita. Aquarium fish species in which there were diagnosed changes in the skin caused by diplomonads were: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. The fish were treated with 250 mg tablets of metronizadole dissolved in water, or metronizadole in a concentration of 6.6 mg per liter of water. The treatment was successful in only 9 out of 45 treated fish. In the others the symptoms reappeared after certain time. It is necessary to determine the prevalence of the infection in aquarium fish in Serbia, and also examine the success of the treatment with metronizadole applied in food or even other possibilities of the treatment.
AB  - Iako komensali u digestivnom sistemu velikog broja vrsta riba, diplomonade predstavljaju veoma značajne oportune uzročnike bolesti. Iz do sada nedovoljno poznatih razloga, mogu da se nekontrolisano razmnožavaju i izazovu promene na koži i unutrašnjim organima kod akvarijumskih riba. Problem predstavlja konfuzija oko nomenklature dvaju najznačajnijih rodova: Spironucleus i Hexamita. Vrste akvarijumskih riba kod kojih su ustanovljene promene na koži prouzrokovane diplomonadama jesu: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. Ribe su tretirane tabletama od 250 mg metronidazola rastvorenim u vodi, odnosno metronidazolom u koncentraciji 6,6 mg na litar vode. Terapija je bila uspešna samo kod 9 od 45 tretiranih riba. Kod ostalih simptomi su se posle izvesnog vremena ponovo pojavljivali. Neophodno je ustanoviti raširenost infekcije kod akvarijumskih riba u Srbiji, a takođe ispitati uspeh lečenja metronidazolom primenjenim u hrani, ili pak druge mogućnosti tretmana.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Diplomonad caused infection in aquarium fish in Serbia
T1  - Infekcija diplomonadama kod akvarijumskih riba u Srbiji
VL  - 70
IS  - 3-4
SP  - 79
EP  - 87
DO  - 10.2298/VETGL1604079M
ER  - 

@article{
author = "Marković, Maja and Radojičić, Marina and Zdravković, Nemanja and Lazić, Marko and Aksentijević, Ksenija",
year = "2016",
abstract = "Although commensals in digestive tract of a large number of fish species, diplomonads represent very significant opportunistic pathogens. For so far unknown reasons, they can proliferate uncontrollably and thus cause changes in the skin and internal organs in aquarium fish. The problem is confusion over nomenclature of the two most important genera: Spironucleus i Hexamita. Aquarium fish species in which there were diagnosed changes in the skin caused by diplomonads were: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. The fish were treated with 250 mg tablets of metronizadole dissolved in water, or metronizadole in a concentration of 6.6 mg per liter of water. The treatment was successful in only 9 out of 45 treated fish. In the others the symptoms reappeared after certain time. It is necessary to determine the prevalence of the infection in aquarium fish in Serbia, and also examine the success of the treatment with metronizadole applied in food or even other possibilities of the treatment., Iako komensali u digestivnom sistemu velikog broja vrsta riba, diplomonade predstavljaju veoma značajne oportune uzročnike bolesti. Iz do sada nedovoljno poznatih razloga, mogu da se nekontrolisano razmnožavaju i izazovu promene na koži i unutrašnjim organima kod akvarijumskih riba. Problem predstavlja konfuzija oko nomenklature dvaju najznačajnijih rodova: Spironucleus i Hexamita. Vrste akvarijumskih riba kod kojih su ustanovljene promene na koži prouzrokovane diplomonadama jesu: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. Ribe su tretirane tabletama od 250 mg metronidazola rastvorenim u vodi, odnosno metronidazolom u koncentraciji 6,6 mg na litar vode. Terapija je bila uspešna samo kod 9 od 45 tretiranih riba. Kod ostalih simptomi su se posle izvesnog vremena ponovo pojavljivali. Neophodno je ustanoviti raširenost infekcije kod akvarijumskih riba u Srbiji, a takođe ispitati uspeh lečenja metronidazolom primenjenim u hrani, ili pak druge mogućnosti tretmana.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Diplomonad caused infection in aquarium fish in Serbia, Infekcija diplomonadama kod akvarijumskih riba u Srbiji",
volume = "70",
number = "3-4",
pages = "79-87",
doi = "10.2298/VETGL1604079M"
}

Marković, M., Radojičić, M., Zdravković, N., Lazić, M.,& Aksentijević, K.. (2016). Diplomonad caused infection in aquarium fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3-4), 79-87.
https://doi.org/10.2298/VETGL1604079M

Marković M, Radojičić M, Zdravković N, Lazić M, Aksentijević K. Diplomonad caused infection in aquarium fish in Serbia. in Veterinarski Glasnik. 2016;70(3-4):79-87.
doi:10.2298/VETGL1604079M .

Marković, Maja, Radojičić, Marina, Zdravković, Nemanja, Lazić, Marko, Aksentijević, Ksenija, "Diplomonad caused infection in aquarium fish in Serbia" in Veterinarski Glasnik, 70, no. 3-4 (2016):79-87,
https://doi.org/10.2298/VETGL1604079M . .

TY  - JOUR
AU  - Rađenović, Milan
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1325
AB  - Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students.
AB  - Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade
T1  - Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu
VL  - 9
IS  - 2
SP  - 17
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1325
ER  - 

@article{
author = "Rađenović, Milan and Ašanin, Jelena and Aksentijević, Ksenija and Mišić, Dušan",
year = "2016",
abstract = "Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students., Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade, Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu",
volume = "9",
number = "2",
pages = "17-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1325"
}

Rađenović, M., Ašanin, J., Aksentijević, K.,& Mišić, D.. (2016). Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(2), 17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325

Rađenović M, Ašanin J, Aksentijević K, Mišić D. Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine. 2016;9(2):17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .

Rađenović, Milan, Ašanin, Jelena, Aksentijević, Ksenija, Mišić, Dušan, "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade" in Arhiv veterinarske medicine, 9, no. 2 (2016):17-28,
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Milivojević, Dušan
AU  - Čolović, Svetlana
AU  - Butorac, Ana
AU  - Cindrić, Mario
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1360
AB  - For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods.
AB  - S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method
T1  - Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode
VL  - 66
IS  - 3
SP  - 304
EP  - 316
DO  - 10.1515/acve-2016-0027
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Milivojević, Dušan and Čolović, Svetlana and Butorac, Ana and Cindrić, Mario and Mišić, Dušan",
year = "2016",
abstract = "For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods., S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method, Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode",
volume = "66",
number = "3",
pages = "304-316",
doi = "10.1515/acve-2016-0027"
}

Aksentijević, K., Ašanin, J., Milivojević, D., Čolović, S., Butorac, A., Cindrić, M.,& Mišić, D.. (2016). Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 304-316.
https://doi.org/10.1515/acve-2016-0027

Aksentijević K, Ašanin J, Milivojević D, Čolović S, Butorac A, Cindrić M, Mišić D. Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd. 2016;66(3):304-316.
doi:10.1515/acve-2016-0027 .

Aksentijević, Ksenija, Ašanin, Jelena, Milivojević, Dušan, Čolović, Svetlana, Butorac, Ana, Cindrić, Mario, Mišić, Dušan, "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method" in Acta Veterinaria-Beograd, 66, no. 3 (2016):304-316,
https://doi.org/10.1515/acve-2016-0027 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Aksentijević, Ksenija
AU  - Stojanović, Dusica B.
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1405
AB  - Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Application of cellulose acetate for controlled release of thymol
VL  - 147
SP  - 344
EP  - 353
DO  - 10.1016/j.carbpol.2016.03.093
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Aksentijević, Ksenija and Stojanović, Dusica B. and Ivanović, Jasna and Žižović, Irena",
year = "2016",
abstract = "Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Application of cellulose acetate for controlled release of thymol",
volume = "147",
pages = "344-353",
doi = "10.1016/j.carbpol.2016.03.093"
}

Milovanović, S., Marković, D., Aksentijević, K., Stojanović, D. B., Ivanović, J.,& Žižović, I.. (2016). Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 147, 344-353.
https://doi.org/10.1016/j.carbpol.2016.03.093

Milovanović S, Marković D, Aksentijević K, Stojanović DB, Ivanović J, Žižović I. Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers. 2016;147:344-353.
doi:10.1016/j.carbpol.2016.03.093 .

Milovanović, Stoja, Marković, Darka, Aksentijević, Ksenija, Stojanović, Dusica B., Ivanović, Jasna, Žižović, Irena, "Application of cellulose acetate for controlled release of thymol" in Carbohydrate Polymers, 147 (2016):344-353,
https://doi.org/10.1016/j.carbpol.2016.03.093 . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Žutić, Milenko
AU  - Katić, Vera
AU  - Krnjaić, Dejan
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/854
AB  - The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml.
AB  - Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution
T1  - Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu
VL  - 66
IS  - 3-4
SP  - 199
EP  - 210
DO  - 10.2298/VETGL1204199A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Žutić, Milenko and Katić, Vera and Krnjaić, Dejan and Milić, Nenad and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml., Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution, Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu",
volume = "66",
number = "3-4",
pages = "199-210",
doi = "10.2298/VETGL1204199A"
}

Ašanin, J., Aksentijević, K., Žutić, M., Katić, V., Krnjaić, D., Milić, N., Ašanin, R.,& Mišić, D.. (2012). Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3-4), 199-210.
https://doi.org/10.2298/VETGL1204199A

Ašanin J, Aksentijević K, Žutić M, Katić V, Krnjaić D, Milić N, Ašanin R, Mišić D. Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik. 2012;66(3-4):199-210.
doi:10.2298/VETGL1204199A .

Ašanin, Jelena, Aksentijević, Ksenija, Žutić, Milenko, Katić, Vera, Krnjaić, Dejan, Milić, Nenad, Ašanin, Ružica, Mišić, Dušan, "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution" in Veterinarski Glasnik, 66, no. 3-4 (2012):199-210,
https://doi.org/10.2298/VETGL1204199A . .

TY  - JOUR
AU  - Marković, Maja
AU  - Ćirković, Miroslav
AU  - Aleksić, Nevenka
AU  - Milošević, Nikolina
AU  - Bjelić-Čabrilo, Olivera
AU  - Ljubojević, Dragana
AU  - Aksentijević, Ksenija
AU  - Radojičić, Marina
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/847
AB  - Posthodiplostomatosis (black spot disease) is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella) and the bighead carp (Aristichthys nobilis) was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned.
AB  - Postodiplostomatoza je oboljenje mlađih kategorija slatkovodnih riba izazvano infekcijom metecerkarijama trematode Posthodiplostomum cuticola. Promene se zapažaju u vidu crnih cista lokalizovanih prvenstveno u koži, subepidermalnom tkivu i na perajima. Najčešće se javlja kod vrsta riba familija Cyprinidae i Cobitidae. Iako je infekcija duži niz godina prisutna u otvorenim vodama, u radu je opisan prvi nalaz infekcije kod riba iz ribnjaka u Srbiji. Postodiplostomatoza je dijagnostikovana kod belog amura (Ctenopharyngodon idella) i sivog tolstolobika (Aristichthys nobilis). Promene su bile karakteristične, makroskopski vidljive kao tamnosiva do crna kružna polja diskoloracije najčešće na perajima. Njihova prosečna veličina iznosila je 946.5±25.19 μm kod belog amura i 944.5±23.50 μm kod tolstolobika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Posthodiplostomatosis in a fishpond in Serbia
T1  - Postodiplostomatoza na ribnjaku u Srbiji
VL  - 62
IS  - 1
SP  - 101
EP  - 109
DO  - 10.2298/AVB1201101M
ER  - 

@article{
author = "Marković, Maja and Ćirković, Miroslav and Aleksić, Nevenka and Milošević, Nikolina and Bjelić-Čabrilo, Olivera and Ljubojević, Dragana and Aksentijević, Ksenija and Radojičić, Marina",
year = "2012",
abstract = "Posthodiplostomatosis (black spot disease) is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella) and the bighead carp (Aristichthys nobilis) was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned., Postodiplostomatoza je oboljenje mlađih kategorija slatkovodnih riba izazvano infekcijom metecerkarijama trematode Posthodiplostomum cuticola. Promene se zapažaju u vidu crnih cista lokalizovanih prvenstveno u koži, subepidermalnom tkivu i na perajima. Najčešće se javlja kod vrsta riba familija Cyprinidae i Cobitidae. Iako je infekcija duži niz godina prisutna u otvorenim vodama, u radu je opisan prvi nalaz infekcije kod riba iz ribnjaka u Srbiji. Postodiplostomatoza je dijagnostikovana kod belog amura (Ctenopharyngodon idella) i sivog tolstolobika (Aristichthys nobilis). Promene su bile karakteristične, makroskopski vidljive kao tamnosiva do crna kružna polja diskoloracije najčešće na perajima. Njihova prosečna veličina iznosila je 946.5±25.19 μm kod belog amura i 944.5±23.50 μm kod tolstolobika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Posthodiplostomatosis in a fishpond in Serbia, Postodiplostomatoza na ribnjaku u Srbiji",
volume = "62",
number = "1",
pages = "101-109",
doi = "10.2298/AVB1201101M"
}

Marković, M., Ćirković, M., Aleksić, N., Milošević, N., Bjelić-Čabrilo, O., Ljubojević, D., Aksentijević, K.,& Radojičić, M.. (2012). Posthodiplostomatosis in a fishpond in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(1), 101-109.
https://doi.org/10.2298/AVB1201101M

Marković M, Ćirković M, Aleksić N, Milošević N, Bjelić-Čabrilo O, Ljubojević D, Aksentijević K, Radojičić M. Posthodiplostomatosis in a fishpond in Serbia. in Acta Veterinaria-Beograd. 2012;62(1):101-109.
doi:10.2298/AVB1201101M .

Marković, Maja, Ćirković, Miroslav, Aleksić, Nevenka, Milošević, Nikolina, Bjelić-Čabrilo, Olivera, Ljubojević, Dragana, Aksentijević, Ksenija, Radojičić, Marina, "Posthodiplostomatosis in a fishpond in Serbia" in Acta Veterinaria-Beograd, 62, no. 1 (2012):101-109,
https://doi.org/10.2298/AVB1201101M . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Zdravković, Nemanja
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/850
AB  - For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains.
AB  - Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals
T1  - Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja
VL  - 62
IS  - 4
SP  - 375
EP  - 384
DO  - 10.2298/AVB1204375A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Zdravković, Nemanja and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains., Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals, Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja",
volume = "62",
number = "4",
pages = "375-384",
doi = "10.2298/AVB1204375A"
}

Ašanin, J., Aksentijević, K., Zdravković, N., Ašanin, R.,& Mišić, D.. (2012). Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 375-384.
https://doi.org/10.2298/AVB1204375A

Ašanin J, Aksentijević K, Zdravković N, Ašanin R, Mišić D. Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd. 2012;62(4):375-384.
doi:10.2298/AVB1204375A .

Ašanin, Jelena, Aksentijević, Ksenija, Zdravković, Nemanja, Ašanin, Ružica, Mišić, Dušan, "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals" in Acta Veterinaria-Beograd, 62, no. 4 (2012):375-384,
https://doi.org/10.2298/AVB1204375A . .

TY  - JOUR
AU  - Tadić, Vanja M.
AU  - Bojović, Dragica
AU  - Arsić, Ivana
AU  - Đorđević, Sofija
AU  - Aksentijević, Ksenija
AU  - Stamenić, Marko
AU  - Janković, Slobodan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/879
AB  - Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
PB  - MDPI, Basel
T2  - Molecules
T1  - Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
VL  - 17
IS  - 3
SP  - 2683
EP  - 2703
DO  - 10.3390/molecules17032683
ER  - 

@article{
author = "Tadić, Vanja M. and Bojović, Dragica and Arsić, Ivana and Đorđević, Sofija and Aksentijević, Ksenija and Stamenić, Marko and Janković, Slobodan",
year = "2012",
abstract = "Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts",
volume = "17",
number = "3",
pages = "2683-2703",
doi = "10.3390/molecules17032683"
}

Tadić, V. M., Bojović, D., Arsić, I., Đorđević, S., Aksentijević, K., Stamenić, M.,& Janković, S.. (2012). Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules
MDPI, Basel., 17(3), 2683-2703.
https://doi.org/10.3390/molecules17032683

Tadić VM, Bojović D, Arsić I, Đorđević S, Aksentijević K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules. 2012;17(3):2683-2703.
doi:10.3390/molecules17032683 .

Tadić, Vanja M., Bojović, Dragica, Arsić, Ivana, Đorđević, Sofija, Aksentijević, Ksenija, Stamenić, Marko, Janković, Slobodan, "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts" in Molecules, 17, no. 3 (2012):2683-2703,
https://doi.org/10.3390/molecules17032683 . .