TY  - CONF
AU  - Ilić, Tamara
AU  - Stepanović, Predrag
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Jovanović, Nemanja M.
AU  - Davidov, Ivana
AU  - Aleksić-Agelidis, Aleksandra
AU  - Aleksić Radojković, Jelena
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3034
AB  - Због бројности популације лисица, распрострањености у различитим еколошким
срединама, интензивирања процеса урбанизације, доступности хране и одморишта,
одсуства предатора и компетитивних врста, ови дивљи месоједи могу да представљају
изворе/резервоаре нематода уринарног тракта за све пријемчиве врсте које деле иста
станишта - ловачки пси, невласнички пси и мачке луталице. Висока преваленција
уринарне капилариозе и спорадична преваленција диоктофимозе код лисица у Европи
и колонизација урбаних подручја лисицама може да повећа ризик од настанка
инфекције домаћих паса и мачака овим хелминтима. Дуг препатентни период, учестала
појава асимптоматских и субклиничких инфекција узрокованих малим бројем паразита
и дисконтинуираном елиминацијом малог броја јаја, отежана дијагностика и
идентификација незрелих јаја паразита, клинички симптоми слични знацима уринарних
бактеријских инфекција и непостојање довољне свести ветеринарских клиничара о
могућем присуству Capillaria plica и Dioctophyma renale код паса и мачака, могу
отежати дијагностиковање ових инфекција. Осим новијих извештаја о налазу врсте C.
plica код лисица (која је утврђена 2020. године на подручју Колубарског округа са
преваленцијом од 70,6%) и ревијалног приказа уринарне капилариозе паса, у
досадашњим истраживањима на подручју Србије није посвећено довољно пажње
изучавању паразитоза уринарног тракта код домаћих месоједа. Због опасности коју D.
renale може да представља за здравље људи и чињенице да у Србији постоје предуслови
за појаву, одржавање и ширење диоктофимозе код месоједа, биолозима, паразитолозима
и ветеринарима потребно је пружити корисне информације од значаја за дијагностику и
праћење нематодоза уринарног тракта код дивљих и домаћих месоједа и указати на
потенцијалне изворе/резервоаре инфекције у Србији. С обзиром да је у Грчкој 2019.
године диоктофимоза дијагностикована код паса, интензивирање ефеката глобалног
загревања може да буде значајан предиспонирајући фактор за појаву ове зоонозе и код
месоједа у Србији. Са клиничко-паразитолошког аспекта важно је да се C. plica и D.
renale уврсте у диференцијалну дијагнозу уринарних обољења паса и мачака, посебно
код животиња са хроничним или рекурентним циститисом и животиња које деле
станишта са инфицираним лисицама.
AB  - Due to the size of the fox population, distribution in different ecological environments,
intensification of the urbanisation process, availability of food and resting places, absence of
predators and competing species, these wild carnivores may represent sources/reservoirs of
urinary tract nematodes for all susceptible species sharing the same habitat - hunting dogs,
stray dogs without owners and cats. The high prevalence of urinary capillariasis and sporadic
prevalence of dioctophimosis in foxes in Europe, as well as the colonisation of urban areas by
foxes, may increase the risk of infection of domestic dogs and cats with these helminths. The
long prepatency period, the frequent occurrence of asymptomatic and subclinical infections
caused by small numbers of parasites, and the discontinuous shedding of small numbers of
eggs, the difficult diagnosis and identification of immature parasite eggs, clinical signs
resembling those of bacterial urinary tract infections, and insufficient awareness among
veterinarians of the possible presence of Capillaria plica and Dioctophyma renale in dogs and
cats may complicate the diagnosis of these infections. Apart from the recent reports on the
discovery of the species C. plica in foxes (detected in the area of Kolubara district in 2020
with a prevalence of 70.6%) and the review presentation of urinary capillariosis in dogs, the
previous research in Serbia did not pay enough attention to the study of the urinary parasite
tract in domestic carnivorous animals. Due to the danger that D. renale may pose to human
health and the fact that conditions for the occurrence, maintenance and spread of
dioctophimosis in carnivores are present in Serbia, it is necessary to provide biologists,
parasitologists and veterinarians with useful information relevant to the diagnosis and
monitoring of urinary nematodes in wild and domestic carnivores and to identify potential
sources/reservoirs of infection in Serbia. Considering that dioctophimosis was diagnosed in
dogs in Greece in 2019, the intensification of the effects of global warming could be an
important predisposing factor for the occurrence of this zoonosis in carnivores in Serbia. From
a clinical parasitological point of view, it is important to include C. plica and D. renale in the
differential diagnosis of urinary tract diseases in dogs and cats, especially in animals with
chronic or recurrent cystitis and in animals sharing their habitat with infected foxes.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Epizootiološki i klinički značaj divljih mesojeda u prenošenju parazitoza urinarnog trakta u Srbiji
T1  - Epidemiological and clinical importance of wild carnivores in the transmission of parasitosis of the urinary tract in Serbia
SP  - 44
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3034
ER  - 

@conference{
author = "Ilić, Tamara and Stepanović, Predrag and Gajić, Bojan and Bogunović, Danica and Jovanović, Nemanja M. and Davidov, Ivana and Aleksić-Agelidis, Aleksandra and Aleksić Radojković, Jelena",
year = "2023",
abstract = "Због бројности популације лисица, распрострањености у различитим еколошким
срединама, интензивирања процеса урбанизације, доступности хране и одморишта,
одсуства предатора и компетитивних врста, ови дивљи месоједи могу да представљају
изворе/резервоаре нематода уринарног тракта за све пријемчиве врсте које деле иста
станишта - ловачки пси, невласнички пси и мачке луталице. Висока преваленција
уринарне капилариозе и спорадична преваленција диоктофимозе код лисица у Европи
и колонизација урбаних подручја лисицама може да повећа ризик од настанка
инфекције домаћих паса и мачака овим хелминтима. Дуг препатентни период, учестала
појава асимптоматских и субклиничких инфекција узрокованих малим бројем паразита
и дисконтинуираном елиминацијом малог броја јаја, отежана дијагностика и
идентификација незрелих јаја паразита, клинички симптоми слични знацима уринарних
бактеријских инфекција и непостојање довољне свести ветеринарских клиничара о
могућем присуству Capillaria plica и Dioctophyma renale код паса и мачака, могу
отежати дијагностиковање ових инфекција. Осим новијих извештаја о налазу врсте C.
plica код лисица (која је утврђена 2020. године на подручју Колубарског округа са
преваленцијом од 70,6%) и ревијалног приказа уринарне капилариозе паса, у
досадашњим истраживањима на подручју Србије није посвећено довољно пажње
изучавању паразитоза уринарног тракта код домаћих месоједа. Због опасности коју D.
renale може да представља за здравље људи и чињенице да у Србији постоје предуслови
за појаву, одржавање и ширење диоктофимозе код месоједа, биолозима, паразитолозима
и ветеринарима потребно је пружити корисне информације од значаја за дијагностику и
праћење нематодоза уринарног тракта код дивљих и домаћих месоједа и указати на
потенцијалне изворе/резервоаре инфекције у Србији. С обзиром да је у Грчкој 2019.
године диоктофимоза дијагностикована код паса, интензивирање ефеката глобалног
загревања може да буде значајан предиспонирајући фактор за појаву ове зоонозе и код
месоједа у Србији. Са клиничко-паразитолошког аспекта важно је да се C. plica и D.
renale уврсте у диференцијалну дијагнозу уринарних обољења паса и мачака, посебно
код животиња са хроничним или рекурентним циститисом и животиња које деле
станишта са инфицираним лисицама., Due to the size of the fox population, distribution in different ecological environments,
intensification of the urbanisation process, availability of food and resting places, absence of
predators and competing species, these wild carnivores may represent sources/reservoirs of
urinary tract nematodes for all susceptible species sharing the same habitat - hunting dogs,
stray dogs without owners and cats. The high prevalence of urinary capillariasis and sporadic
prevalence of dioctophimosis in foxes in Europe, as well as the colonisation of urban areas by
foxes, may increase the risk of infection of domestic dogs and cats with these helminths. The
long prepatency period, the frequent occurrence of asymptomatic and subclinical infections
caused by small numbers of parasites, and the discontinuous shedding of small numbers of
eggs, the difficult diagnosis and identification of immature parasite eggs, clinical signs
resembling those of bacterial urinary tract infections, and insufficient awareness among
veterinarians of the possible presence of Capillaria plica and Dioctophyma renale in dogs and
cats may complicate the diagnosis of these infections. Apart from the recent reports on the
discovery of the species C. plica in foxes (detected in the area of Kolubara district in 2020
with a prevalence of 70.6%) and the review presentation of urinary capillariosis in dogs, the
previous research in Serbia did not pay enough attention to the study of the urinary parasite
tract in domestic carnivorous animals. Due to the danger that D. renale may pose to human
health and the fact that conditions for the occurrence, maintenance and spread of
dioctophimosis in carnivores are present in Serbia, it is necessary to provide biologists,
parasitologists and veterinarians with useful information relevant to the diagnosis and
monitoring of urinary nematodes in wild and domestic carnivores and to identify potential
sources/reservoirs of infection in Serbia. Considering that dioctophimosis was diagnosed in
dogs in Greece in 2019, the intensification of the effects of global warming could be an
important predisposing factor for the occurrence of this zoonosis in carnivores in Serbia. From
a clinical parasitological point of view, it is important to include C. plica and D. renale in the
differential diagnosis of urinary tract diseases in dogs and cats, especially in animals with
chronic or recurrent cystitis and in animals sharing their habitat with infected foxes.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Epizootiološki i klinički značaj divljih mesojeda u prenošenju parazitoza urinarnog trakta u Srbiji, Epidemiological and clinical importance of wild carnivores in the transmission of parasitosis of the urinary tract in Serbia",
pages = "44-45",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3034"
}

Ilić, T., Stepanović, P., Gajić, B., Bogunović, D., Jovanović, N. M., Davidov, I., Aleksić-Agelidis, A.,& Aleksić Radojković, J.. (2023). Epizootiološki i klinički značaj divljih mesojeda u prenošenju parazitoza urinarnog trakta u Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 44-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3034

Ilić T, Stepanović P, Gajić B, Bogunović D, Jovanović NM, Davidov I, Aleksić-Agelidis A, Aleksić Radojković J. Epizootiološki i klinički značaj divljih mesojeda u prenošenju parazitoza urinarnog trakta u Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:44-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3034 .

Ilić, Tamara, Stepanović, Predrag, Gajić, Bojan, Bogunović, Danica, Jovanović, Nemanja M., Davidov, Ivana, Aleksić-Agelidis, Aleksandra, Aleksić Radojković, Jelena, "Epizootiološki i klinički značaj divljih mesojeda u prenošenju parazitoza urinarnog trakta u Srbiji" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):44-45,
https://hdl.handle.net/21.15107/rcub_veterinar_3034 .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Bogunović, Danica
AU  - Nenadović, Katarina
AU  - Gajić, Bojan
AU  - Dimitrijević, Sanda
AU  - Popović, Gorana
AU  - Kulišić, Zoran
AU  - Milosavljević, Petar
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2558
AB  - Purpose: Data on gastrointestinal infections in horses in Southeastern Europe are limited; thus, this study aimed to add to the existing knowledge on this topic by reporting on the prevalence of intestinal parasites of horses in the territory of the Republic of Serbia. Methods: In the period from April 2017 to December 2018, parasitological examination of 548 samples from horses of different breed, age and sex from four regions and 18 districts of the country was performed. Coprological diagnostic was done by using qualitative methods without concentration and qualitative methods with concentration of parasitic elements. Quantification of the obtained results was performed using semi-qualitative faecal egg count. Results: Four helminthoses were detected in the examined samples: P. equorum (8.57%), O. equi (3.65%), strongylid eggs (71.17%) and Anoplocephala spp. (0.91%). The total prevalence of helminthoses was 77.19%. Monoinfections were significantly more present 70.07% compared to coinfections (7.12%). The highest prevalence of helminthoses was detected in free-ranging horses (93.10%—27/29), in autumn 86.67% (117/135) and winter 79.71% (165/207), in Šumadija and West Serbia region (100%), and in the youngest category (100%). Significant difference (p < 0.001) was detected in the prevalence of monoinfections by strongylids and O. equi and also coinfections by strongylid/P. equorum between horses of different age categories. Conclusion: Obtained results are of great contribution to clinical parasitology and pathology, especially from the aspect of animal health, welfare and preservation of horse population.
PB  - Springer Science and Business Media
T2  - Acta Parasitologica
T1  - Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence
VL  - 68
SP  - 56
EP  - 69
DO  - 10.1007/s11686-022-00636-z
ER  - 

@article{
author = "Ilić, Tamara and Bogunović, Danica and Nenadović, Katarina and Gajić, Bojan and Dimitrijević, Sanda and Popović, Gorana and Kulišić, Zoran and Milosavljević, Petar",
year = "2023",
abstract = "Purpose: Data on gastrointestinal infections in horses in Southeastern Europe are limited; thus, this study aimed to add to the existing knowledge on this topic by reporting on the prevalence of intestinal parasites of horses in the territory of the Republic of Serbia. Methods: In the period from April 2017 to December 2018, parasitological examination of 548 samples from horses of different breed, age and sex from four regions and 18 districts of the country was performed. Coprological diagnostic was done by using qualitative methods without concentration and qualitative methods with concentration of parasitic elements. Quantification of the obtained results was performed using semi-qualitative faecal egg count. Results: Four helminthoses were detected in the examined samples: P. equorum (8.57%), O. equi (3.65%), strongylid eggs (71.17%) and Anoplocephala spp. (0.91%). The total prevalence of helminthoses was 77.19%. Monoinfections were significantly more present 70.07% compared to coinfections (7.12%). The highest prevalence of helminthoses was detected in free-ranging horses (93.10%—27/29), in autumn 86.67% (117/135) and winter 79.71% (165/207), in Šumadija and West Serbia region (100%), and in the youngest category (100%). Significant difference (p < 0.001) was detected in the prevalence of monoinfections by strongylids and O. equi and also coinfections by strongylid/P. equorum between horses of different age categories. Conclusion: Obtained results are of great contribution to clinical parasitology and pathology, especially from the aspect of animal health, welfare and preservation of horse population.",
publisher = "Springer Science and Business Media",
journal = "Acta Parasitologica",
title = "Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence",
volume = "68",
pages = "56-69",
doi = "10.1007/s11686-022-00636-z"
}

Ilić, T., Bogunović, D., Nenadović, K., Gajić, B., Dimitrijević, S., Popović, G., Kulišić, Z.,& Milosavljević, P.. (2023). Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence. in Acta Parasitologica
Springer Science and Business Media., 68, 56-69.
https://doi.org/10.1007/s11686-022-00636-z

Ilić T, Bogunović D, Nenadović K, Gajić B, Dimitrijević S, Popović G, Kulišić Z, Milosavljević P. Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence. in Acta Parasitologica. 2023;68:56-69.
doi:10.1007/s11686-022-00636-z .

Ilić, Tamara, Bogunović, Danica, Nenadović, Katarina, Gajić, Bojan, Dimitrijević, Sanda, Popović, Gorana, Kulišić, Zoran, Milosavljević, Petar, "Gastrointestinal Helminths in Horses in Serbia and Various Factors Affecting the Prevalence" in Acta Parasitologica, 68 (2023):56-69,
https://doi.org/10.1007/s11686-022-00636-z . .

TY  - CONF
AU  - Ilić, Tamara
AU  - Petrović, Tamaš
AU  - Stepanović, Predrag
AU  - Bogunović, Danica
AU  - Gajić, Bojan
AU  - Kulišić, Zoran
AU  - Ristić, Marko
AU  - Hadži-Milić, Milan
AU  - Dimitrijević, Sanda
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3292
AB  - Рад представља ретроспективни извештај и резиме истраживања спроведених по пројекту
ТР31084 у периоду од 2015. – 2020. године. Студија има за циљ да укаже на повећање
преваленције нематода са зоонозним потенцијалом код домаћих и дивљих месоједа на
подручју Србије. Приказана епизоотиолошка ситуација настала је као последица
глобалног загревања и активних миграција власника са својим љубимцима у земље
региона и Европе, од којих многе већ представљају ендемска подручја дијагностикованих
узрочника. Наведени фактори су имали за последицу повећање заступљености појединих
кардиореспираторних, окуларних и интестиналних паразитоза, од којих су неке попримиле
и ендемски карактер на подручју Србије. У популацији испитиваних домаћих месоједа
(власнички пси и мачке, службени пси и пси из прихватилишта) дијагностикована је
највећа преваленција Dirofilaria immitis (68,00%) код службених паса са подручја Београда
и Thelazia callipaeda (47,50%) код власничких паса из Борског округа. Од интестиналних
нематода најзаступљеније су биле Toxocara canis (42,85%) код паса из Прихватилишта у
Земуну и Dipylidium caninum (21,49 - 24,70%) код власничких мачака и паса са територије
Града Београда. Код испитиваних дивљих месоједа (лисице и шакали) доминирало је
присуство респираторне нематоде Capillaria aerophila (74,04%) код лисица из Зајечарског
округа. Токсокароза лисица (84,05%) и анкилостоматидоза шакала (40,90%), биле су
најфреквентније интестиналне нематодозе дивљих месоједа са подручја Републике Србије
обухваћених спроведеним паразитолошким скринингом. Урбанизација градова у Србији
доводи до ширења градског појаса на периферне делове града и викенд насеља, на којима
су до недавно боравиле само лисице. Ширењем градског појаса долази до блиског контакта
паса луталица и власничких дворишних паса са лисицама. Резултат таквог контакта је
значајно мењање паразитофауне паса, тако да они постају извор нових узрочника, које
касније преко свог гастроинтестиналног тракта елиминишу на јавне површине и
представљају извор инфекције за људску популацију. У Србији, као и у многим земљама
Европе, законски је регулисана вакцинација лисица против беснила (путем мамаца).
Захваљујући томе, беснило је стављено под контролу, али се повећао број лисица, а самим
тим и преваленција паразита за које лисице представљају изворе/резервоаре инфекције. То
је само додатни фактор који у ланцу: лисица - пас луталица - власнички пас - човек,
значајно повећава ризик и тенденцију за настанак инфекције људи цревним паразитима
дивљих и домаћих месоједа.
AB  - The paper presents a retrospective report and a summary of the research conducted under the
TR31084 project in the period 2015-2020. The study aims to point to an increase in the
prevalence of nematodes with zoonotic potential in domestic and wild carnivores in Serbia.
The presented epizootiological situation arose as a result of global warming and active
migration of owners with their pets to countries in the region and Europe, many of which
already represent endemic areas of diagnosed pathogens. These factors have led to an increase
in the prevalence of certain cardiorespiratory, ocular and intestinal parasitoses, some of which
have also become endemic in Serbia. The population of domestic carnivores surveyed (owner
dogs and cats, service dogs and shelter dogs) was diagnosed with the highest prevalence of
Dirofilaria immitis (68.00%) in service dogs from Belgrade and Thelazia callipaeda (47.50%)
in owner dogs from Bor district. Of the intestinal nematodes, the most frequent were Toxocara
canis (42.85%) in dogs from the shelter in Zemun and Dipylidium caninum (21.49 - 24.70%)
in owner cats and dogs from the territory of the City of Belgrade. In the wild carnivores (foxes
and jackals) examined, the presence of the respiratory nematode Capillaria aerophila
(74.04%) was dominated by foxes from the Zaječar district. Toxarosis of foxes (84.05%) and
ancylostomatidosis of jackals (40.90%) were the most frequent intestinal nematodes of wild
carnivores in the Republic of Serbia, covered by parasitological screening. Urbanization of
cities in Serbia has led to the expansion of the urban belt to the peripheral parts of the city and
to the cottage settlements, which until recently were occupied only by foxes. With the
widening of the city belt, close contact of stray dogs and owner's backyard dogs is handcuffed.
The result of such contact is a significant alteration of the parasitofauna of dogs, so that they
become a source of new pathogens, which later through their gastrointestinal tract eliminate to
public areas and are a source of infection for the human population. In Serbia, as in many
European countries, vaccination of foxes against rabies (through bait) is legally regulated. As
a result, rabies has been brought under control, but the number of foxes has increased, and
therefore the prevalence of parasites for which foxes are sources / reservoirs of infection. This
is just an additional factor in the chain: fox - stray dog - owner dog - human, significantly
increases the risk and the tendency for humans to become infected with intestinal parasites of
wild and domestic carnivores.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
T1  - Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji
T1  - Epizootiological situation of more important zoonotic helminthoses of domestic and wild carnivores in Serbia
SP  - 40
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3292
ER  - 

@conference{
author = "Ilić, Tamara and Petrović, Tamaš and Stepanović, Predrag and Bogunović, Danica and Gajić, Bojan and Kulišić, Zoran and Ristić, Marko and Hadži-Milić, Milan and Dimitrijević, Sanda",
year = "2021",
abstract = "Рад представља ретроспективни извештај и резиме истраживања спроведених по пројекту
ТР31084 у периоду од 2015. – 2020. године. Студија има за циљ да укаже на повећање
преваленције нематода са зоонозним потенцијалом код домаћих и дивљих месоједа на
подручју Србије. Приказана епизоотиолошка ситуација настала је као последица
глобалног загревања и активних миграција власника са својим љубимцима у земље
региона и Европе, од којих многе већ представљају ендемска подручја дијагностикованих
узрочника. Наведени фактори су имали за последицу повећање заступљености појединих
кардиореспираторних, окуларних и интестиналних паразитоза, од којих су неке попримиле
и ендемски карактер на подручју Србије. У популацији испитиваних домаћих месоједа
(власнички пси и мачке, службени пси и пси из прихватилишта) дијагностикована је
највећа преваленција Dirofilaria immitis (68,00%) код службених паса са подручја Београда
и Thelazia callipaeda (47,50%) код власничких паса из Борског округа. Од интестиналних
нематода најзаступљеније су биле Toxocara canis (42,85%) код паса из Прихватилишта у
Земуну и Dipylidium caninum (21,49 - 24,70%) код власничких мачака и паса са територије
Града Београда. Код испитиваних дивљих месоједа (лисице и шакали) доминирало је
присуство респираторне нематоде Capillaria aerophila (74,04%) код лисица из Зајечарског
округа. Токсокароза лисица (84,05%) и анкилостоматидоза шакала (40,90%), биле су
најфреквентније интестиналне нематодозе дивљих месоједа са подручја Републике Србије
обухваћених спроведеним паразитолошким скринингом. Урбанизација градова у Србији
доводи до ширења градског појаса на периферне делове града и викенд насеља, на којима
су до недавно боравиле само лисице. Ширењем градског појаса долази до блиског контакта
паса луталица и власничких дворишних паса са лисицама. Резултат таквог контакта је
значајно мењање паразитофауне паса, тако да они постају извор нових узрочника, које
касније преко свог гастроинтестиналног тракта елиминишу на јавне површине и
представљају извор инфекције за људску популацију. У Србији, као и у многим земљама
Европе, законски је регулисана вакцинација лисица против беснила (путем мамаца).
Захваљујући томе, беснило је стављено под контролу, али се повећао број лисица, а самим
тим и преваленција паразита за које лисице представљају изворе/резервоаре инфекције. То
је само додатни фактор који у ланцу: лисица - пас луталица - власнички пас - човек,
значајно повећава ризик и тенденцију за настанак инфекције људи цревним паразитима
дивљих и домаћих месоједа., The paper presents a retrospective report and a summary of the research conducted under the
TR31084 project in the period 2015-2020. The study aims to point to an increase in the
prevalence of nematodes with zoonotic potential in domestic and wild carnivores in Serbia.
The presented epizootiological situation arose as a result of global warming and active
migration of owners with their pets to countries in the region and Europe, many of which
already represent endemic areas of diagnosed pathogens. These factors have led to an increase
in the prevalence of certain cardiorespiratory, ocular and intestinal parasitoses, some of which
have also become endemic in Serbia. The population of domestic carnivores surveyed (owner
dogs and cats, service dogs and shelter dogs) was diagnosed with the highest prevalence of
Dirofilaria immitis (68.00%) in service dogs from Belgrade and Thelazia callipaeda (47.50%)
in owner dogs from Bor district. Of the intestinal nematodes, the most frequent were Toxocara
canis (42.85%) in dogs from the shelter in Zemun and Dipylidium caninum (21.49 - 24.70%)
in owner cats and dogs from the territory of the City of Belgrade. In the wild carnivores (foxes
and jackals) examined, the presence of the respiratory nematode Capillaria aerophila
(74.04%) was dominated by foxes from the Zaječar district. Toxarosis of foxes (84.05%) and
ancylostomatidosis of jackals (40.90%) were the most frequent intestinal nematodes of wild
carnivores in the Republic of Serbia, covered by parasitological screening. Urbanization of
cities in Serbia has led to the expansion of the urban belt to the peripheral parts of the city and
to the cottage settlements, which until recently were occupied only by foxes. With the
widening of the city belt, close contact of stray dogs and owner's backyard dogs is handcuffed.
The result of such contact is a significant alteration of the parasitofauna of dogs, so that they
become a source of new pathogens, which later through their gastrointestinal tract eliminate to
public areas and are a source of infection for the human population. In Serbia, as in many
European countries, vaccination of foxes against rabies (through bait) is legally regulated. As
a result, rabies has been brought under control, but the number of foxes has increased, and
therefore the prevalence of parasites for which foxes are sources / reservoirs of infection. This
is just an additional factor in the chain: fox - stray dog - owner dog - human, significantly
increases the risk and the tendency for humans to become infected with intestinal parasites of
wild and domestic carnivores.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021",
title = "Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji, Epizootiological situation of more important zoonotic helminthoses of domestic and wild carnivores in Serbia",
pages = "40-41",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3292"
}

Ilić, T., Petrović, T., Stepanović, P., Bogunović, D., Gajić, B., Kulišić, Z., Ristić, M., Hadži-Milić, M.,& Dimitrijević, S.. (2021). Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 40-41.
https://hdl.handle.net/21.15107/rcub_veterinar_3292

Ilić T, Petrović T, Stepanović P, Bogunović D, Gajić B, Kulišić Z, Ristić M, Hadži-Milić M, Dimitrijević S. Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021. 2021;:40-41.
https://hdl.handle.net/21.15107/rcub_veterinar_3292 .

Ilić, Tamara, Petrović, Tamaš, Stepanović, Predrag, Bogunović, Danica, Gajić, Bojan, Kulišić, Zoran, Ristić, Marko, Hadži-Milić, Milan, Dimitrijević, Sanda, "Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji" in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021 (2021):40-41,
https://hdl.handle.net/21.15107/rcub_veterinar_3292 .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Rogošić, Milan
AU  - Gajić, Bojan
AU  - Aleksić, Jelena
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2216
AB  - Background. Urinary capillariosis in dogs is caused by Capillaria plica (syn. Pearsonema plica), a ubiquitous parasitic nematode resembling a string which belongs to the family Capillariidae. It parasitizes the feline, canine and musteline urinary bladder, and has been found in ureters and renal pelvises as well. C. plica has an indirect life cycle, with earthworms (Lumbricina) as intermediate hosts and domestic and wild animals (dog, cat, fox and wolf) as primary hosts. Infection of primary hosts occurs via ingestion of earthworms that contain infective first stadium (L1) larvae. An alternative path of infection for primary hosts is assumed to be ingestion of soil contaminated by infectious larvae derived from decomposed earthworms. Infection is mostly asymptomatic, but the clinical picture presents with pollakiuria, dysuria, haematuria, polydipsia, incontinence and/or fever. 
Scope and Approach. The aim of this review is to highlight the importance of urinary capillariosis in dogs. Since the health care of wild and domestic carnivores is extremely important, this review provides information about the morphology, biology and epizootiology of the C. plica nematode. Due to the importance of this disease for clinicians and increased disease prevalence during the last decade in many countries, this review presents the latest information on the pathogenesis, clinical signs, diagnosis, treatment and prevention of this infection. 
Key Findings and Conclusions. Capillariosis is usually accidentally diagnosed due to the nonspecific clinical signs and there is no treatment of choice. Practitioners should consider latent urinary capillariosis infection as a possible cause while examining for urinary tract diseases.
AB  - Uvod. Urinarnu kapilariozu pasa uzrokuje Capillaria plica (syn. Pearsonema plica), ubikvitarna nematoda končastog oblika iz familije Capillariidae. Uzročnik parazitira u mokraćnoj bešici kanida, felida i mustelida, a može da se ustanovi u ureterima i bubrežnoj karlici. Capillaria plica ima indirektan životni ciklus koji uključuje kišne gliste (Lumbricina) kao prelazne domaćine i domaće i divlje životinje (pas, mačka, lisica, vuk) kao prave domaćine. Infekcija pravog domaćina nastaje unošenjem kišnih glista koje sadrže infektivnu larvu prvog stadijuma (L1). Pretpostavlja se da alternativni put infekcije za životinje može da bude ingestija zemljišta kontaminiranog infektivnim larvama poreklom od kišnih glista koje su podlegle procesu dekompozicije. Infekcija je najčešće asimptomatska, a u slučaju ispoljavanja kliničke slike simptomi su polakiurija, disurija, hematurija, polidipsija, inkontinencija urina i groznica 
Cilj i pristup. Cilj rada je da pruži informacije o morfologiji, biologiji i epizootiologiji nematode Capilaria plica kao i da ukaže na mogućnost postojanja infekcije kod lovačkih 
i nevlasničkih pasa. Zbog značaja oboljenja za kliničare i povećanja prevalencije tokom poslednje decenije u zemljama u okruženju, u radu su iznete najnovije informacije o patogenezi, kliničkim simptomima, dijagnostici, lečenju i prevenciji ove nematodoze. 
Ključni nalazi i zaključak. Dijagnoza kapilarioze se najčešće postavlja slučajnim nalazom parazita zbog nespecifičnе kliničke slike. U slučaju sumnje na oboljenja urinarnog trakta, treba diferencijalno dijagnostički isključiti latentnu infekciju ovom nematodom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Urinary capillariosis in dogs
T1  - Urinarna kapilarioza kod pasa
VL  - 75
IS  - 1
SP  - 20
EP  - 32
DO  - 10.2298/VETGL191009003I
ER  - 

@article{
author = "Ilić, Tamara and Rogošić, Milan and Gajić, Bojan and Aleksić, Jelena",
year = "2021",
abstract = "Background. Urinary capillariosis in dogs is caused by Capillaria plica (syn. Pearsonema plica), a ubiquitous parasitic nematode resembling a string which belongs to the family Capillariidae. It parasitizes the feline, canine and musteline urinary bladder, and has been found in ureters and renal pelvises as well. C. plica has an indirect life cycle, with earthworms (Lumbricina) as intermediate hosts and domestic and wild animals (dog, cat, fox and wolf) as primary hosts. Infection of primary hosts occurs via ingestion of earthworms that contain infective first stadium (L1) larvae. An alternative path of infection for primary hosts is assumed to be ingestion of soil contaminated by infectious larvae derived from decomposed earthworms. Infection is mostly asymptomatic, but the clinical picture presents with pollakiuria, dysuria, haematuria, polydipsia, incontinence and/or fever. 
Scope and Approach. The aim of this review is to highlight the importance of urinary capillariosis in dogs. Since the health care of wild and domestic carnivores is extremely important, this review provides information about the morphology, biology and epizootiology of the C. plica nematode. Due to the importance of this disease for clinicians and increased disease prevalence during the last decade in many countries, this review presents the latest information on the pathogenesis, clinical signs, diagnosis, treatment and prevention of this infection. 
Key Findings and Conclusions. Capillariosis is usually accidentally diagnosed due to the nonspecific clinical signs and there is no treatment of choice. Practitioners should consider latent urinary capillariosis infection as a possible cause while examining for urinary tract diseases., Uvod. Urinarnu kapilariozu pasa uzrokuje Capillaria plica (syn. Pearsonema plica), ubikvitarna nematoda končastog oblika iz familije Capillariidae. Uzročnik parazitira u mokraćnoj bešici kanida, felida i mustelida, a može da se ustanovi u ureterima i bubrežnoj karlici. Capillaria plica ima indirektan životni ciklus koji uključuje kišne gliste (Lumbricina) kao prelazne domaćine i domaće i divlje životinje (pas, mačka, lisica, vuk) kao prave domaćine. Infekcija pravog domaćina nastaje unošenjem kišnih glista koje sadrže infektivnu larvu prvog stadijuma (L1). Pretpostavlja se da alternativni put infekcije za životinje može da bude ingestija zemljišta kontaminiranog infektivnim larvama poreklom od kišnih glista koje su podlegle procesu dekompozicije. Infekcija je najčešće asimptomatska, a u slučaju ispoljavanja kliničke slike simptomi su polakiurija, disurija, hematurija, polidipsija, inkontinencija urina i groznica 
Cilj i pristup. Cilj rada je da pruži informacije o morfologiji, biologiji i epizootiologiji nematode Capilaria plica kao i da ukaže na mogućnost postojanja infekcije kod lovačkih 
i nevlasničkih pasa. Zbog značaja oboljenja za kliničare i povećanja prevalencije tokom poslednje decenije u zemljama u okruženju, u radu su iznete najnovije informacije o patogenezi, kliničkim simptomima, dijagnostici, lečenju i prevenciji ove nematodoze. 
Ključni nalazi i zaključak. Dijagnoza kapilarioze se najčešće postavlja slučajnim nalazom parazita zbog nespecifičnе kliničke slike. U slučaju sumnje na oboljenja urinarnog trakta, treba diferencijalno dijagnostički isključiti latentnu infekciju ovom nematodom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Urinary capillariosis in dogs, Urinarna kapilarioza kod pasa",
volume = "75",
number = "1",
pages = "20-32",
doi = "10.2298/VETGL191009003I"
}

Ilić, T., Rogošić, M., Gajić, B.,& Aleksić, J.. (2021). Urinary capillariosis in dogs. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 20-32.
https://doi.org/10.2298/VETGL191009003I

Ilić T, Rogošić M, Gajić B, Aleksić J. Urinary capillariosis in dogs. in Veterinarski Glasnik. 2021;75(1):20-32.
doi:10.2298/VETGL191009003I .

Ilić, Tamara, Rogošić, Milan, Gajić, Bojan, Aleksić, Jelena, "Urinary capillariosis in dogs" in Veterinarski Glasnik, 75, no. 1 (2021):20-32,
https://doi.org/10.2298/VETGL191009003I . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Nišavić, Uroš
AU  - Gajić, Bojan
AU  - Nenadović, Katarina
AU  - Ristić, Marko
AU  - Stanojević, Dejan
AU  - Dimitrijević, Sanda
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2062
AB  - Data on endoparasitic infections in dogs from dog shelters in Southeastern Europe are limited; thus, this study aimed to add to the existing knowledge on this topic by reporting on the prevalence of intestinal parasites in dogs from public dog shelters in the Republic of Serbia. In 2017 and 2018, individual and pooled fecal samples, were collected from 1267 dogs from six shelters. All samples were qualitatively examined for parasites using flotation tests. Seven taxa of intestinal parasites were identified: Cystoisospora spp., ascarids: Toxocara canis and Toxascaris leonina, hookworms, Trichuris vulpis, taeniids and Dipylidium caninum. The overall prevalence of intestinal parasites was 58. 3 % (78. 1 % in young dogs and 53.1 % in adult dogs). The parasites detected in both young (1 year old) were Cystoisospora spp. (20 % and 4.9 %), T. canis (33.5 % and 14.7 %), T. leonina (7.7 % and 2.3 %), and hookworms (16.9 % and 15 %), respectively. However, T. vulpis (9.6 %), taeniids (1.3 %), and D. caninum (5.4 %) were detected only in adult dogs. In the Belgrade shelter, young dogs had a higher prevalence of endoparasitic infections (18.9 %, 49/260) than adult dogs (14.8 %, 149/1007). In the Subotica, Jagodina and Niš shelters, young dogs had significantly higher (p < 0.001 and p < 0.05, respectively) prevalence of endoparasitic infections (10 %, 12.3 % and 14.6 %) than adult dogs (5.3 %, 8 % and 7.2 %). These results will be useful for establishing health care programs in dog shelters and implementing effective strategies for the control of intestinal parasites.
PB  - Elsevier
T2  - Comparative Immunology, Microbiology and Infectious Diseases
T1  - Prevalence of intestinal parasites in dogs from public shelters in Serbia
VL  - 76
SP  - 101653
DO  - 10.1016/j.cimid.2021.101653
ER  - 

@article{
author = "Ilić, Tamara and Nišavić, Uroš and Gajić, Bojan and Nenadović, Katarina and Ristić, Marko and Stanojević, Dejan and Dimitrijević, Sanda",
year = "2021",
abstract = "Data on endoparasitic infections in dogs from dog shelters in Southeastern Europe are limited; thus, this study aimed to add to the existing knowledge on this topic by reporting on the prevalence of intestinal parasites in dogs from public dog shelters in the Republic of Serbia. In 2017 and 2018, individual and pooled fecal samples, were collected from 1267 dogs from six shelters. All samples were qualitatively examined for parasites using flotation tests. Seven taxa of intestinal parasites were identified: Cystoisospora spp., ascarids: Toxocara canis and Toxascaris leonina, hookworms, Trichuris vulpis, taeniids and Dipylidium caninum. The overall prevalence of intestinal parasites was 58. 3 % (78. 1 % in young dogs and 53.1 % in adult dogs). The parasites detected in both young (1 year old) were Cystoisospora spp. (20 % and 4.9 %), T. canis (33.5 % and 14.7 %), T. leonina (7.7 % and 2.3 %), and hookworms (16.9 % and 15 %), respectively. However, T. vulpis (9.6 %), taeniids (1.3 %), and D. caninum (5.4 %) were detected only in adult dogs. In the Belgrade shelter, young dogs had a higher prevalence of endoparasitic infections (18.9 %, 49/260) than adult dogs (14.8 %, 149/1007). In the Subotica, Jagodina and Niš shelters, young dogs had significantly higher (p < 0.001 and p < 0.05, respectively) prevalence of endoparasitic infections (10 %, 12.3 % and 14.6 %) than adult dogs (5.3 %, 8 % and 7.2 %). These results will be useful for establishing health care programs in dog shelters and implementing effective strategies for the control of intestinal parasites.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Prevalence of intestinal parasites in dogs from public shelters in Serbia",
volume = "76",
pages = "101653",
doi = "10.1016/j.cimid.2021.101653"
}

Ilić, T., Nišavić, U., Gajić, B., Nenadović, K., Ristić, M., Stanojević, D.,& Dimitrijević, S.. (2021). Prevalence of intestinal parasites in dogs from public shelters in Serbia. in Comparative Immunology, Microbiology and Infectious Diseases
Elsevier., 76, 101653.
https://doi.org/10.1016/j.cimid.2021.101653

Ilić T, Nišavić U, Gajić B, Nenadović K, Ristić M, Stanojević D, Dimitrijević S. Prevalence of intestinal parasites in dogs from public shelters in Serbia. in Comparative Immunology, Microbiology and Infectious Diseases. 2021;76:101653.
doi:10.1016/j.cimid.2021.101653 .

Ilić, Tamara, Nišavić, Uroš, Gajić, Bojan, Nenadović, Katarina, Ristić, Marko, Stanojević, Dejan, Dimitrijević, Sanda, "Prevalence of intestinal parasites in dogs from public shelters in Serbia" in Comparative Immunology, Microbiology and Infectious Diseases, 76 (2021):101653,
https://doi.org/10.1016/j.cimid.2021.101653 . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Petrović, Tamaš
AU  - Stepanović, Predrag
AU  - Bogunović, Danica
AU  - Gajić, Bojan
AU  - Kulišić, Zoran
AU  - Ristić, Marko
AU  - Hadži-Milić, Milan
AU  - Dimitrijević, Sanda
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3293
AB  - In the last decade, as the result of climate changes, there have been considerable
changes in the parasitofauna of domestic and wild carnivores. Th e
prevalence of the existing parasitic species has varied signifi cantly, showing
an increasing tendency, and some parasitic species not present before
in this epizootiologic territory have been diagnosed as well. It is thought
that the reason for such an epizootiological situation is increased presence
of owners with their pet animals in the regions endemic for particular
zoonotic helminthoses during summer holidays and touristic visits. Th is
tendency has become especially conspicuous in the last several years characterized
by warm winters and very hot summers due to global warming
eff ects, with abundant atmospheric precipitation. Oral vaccination of foxes
against rabies, regulated in Serbia by appropriate laws since 2010, has led to
an increased number of foxes and rise of prevalence of the parasitic diseases for which foxes represent the infection source/reservoir. Continued urbanization
of Serbian cities, with the extension of urban belts into the suburbia
and recreational (“weekend”) settlements, lead to a closer contact of street
dogs and owned dogs with foxes, which results in a signifi cant change in
the parasitic fauna of dogs. It is an additional factor, which in the chain
fox - street dog - owned dog - human increases the risk and tendency for
the occurrence of human infections with zoonotic endoparasites of wild
and domestic carnivores. In order to reliably predict the degree of spread of
particular zoonotic helminthoses in particular regions in Serbia, for which
wild carnivores represent the infection source, it is necessary to institute
continued monitoring of the parasitic fauna in this type of wild animals.
PB  - Novi Sad : Scientific Institute for Veterinary Medicine
T2  - Arhiv veterinarske medicine
T1  - Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia
VL  - 13
IS  - 1
SP  - 5
EP  - 28
DO  - 10.46784/e-avm.v13i1.238
ER  - 

@article{
author = "Ilić, Tamara and Petrović, Tamaš and Stepanović, Predrag and Bogunović, Danica and Gajić, Bojan and Kulišić, Zoran and Ristić, Marko and Hadži-Milić, Milan and Dimitrijević, Sanda",
year = "2020",
abstract = "In the last decade, as the result of climate changes, there have been considerable
changes in the parasitofauna of domestic and wild carnivores. Th e
prevalence of the existing parasitic species has varied signifi cantly, showing
an increasing tendency, and some parasitic species not present before
in this epizootiologic territory have been diagnosed as well. It is thought
that the reason for such an epizootiological situation is increased presence
of owners with their pet animals in the regions endemic for particular
zoonotic helminthoses during summer holidays and touristic visits. Th is
tendency has become especially conspicuous in the last several years characterized
by warm winters and very hot summers due to global warming
eff ects, with abundant atmospheric precipitation. Oral vaccination of foxes
against rabies, regulated in Serbia by appropriate laws since 2010, has led to
an increased number of foxes and rise of prevalence of the parasitic diseases for which foxes represent the infection source/reservoir. Continued urbanization
of Serbian cities, with the extension of urban belts into the suburbia
and recreational (“weekend”) settlements, lead to a closer contact of street
dogs and owned dogs with foxes, which results in a signifi cant change in
the parasitic fauna of dogs. It is an additional factor, which in the chain
fox - street dog - owned dog - human increases the risk and tendency for
the occurrence of human infections with zoonotic endoparasites of wild
and domestic carnivores. In order to reliably predict the degree of spread of
particular zoonotic helminthoses in particular regions in Serbia, for which
wild carnivores represent the infection source, it is necessary to institute
continued monitoring of the parasitic fauna in this type of wild animals.",
publisher = "Novi Sad : Scientific Institute for Veterinary Medicine",
journal = "Arhiv veterinarske medicine",
title = "Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia",
volume = "13",
number = "1",
pages = "5-28",
doi = "10.46784/e-avm.v13i1.238"
}

Ilić, T., Petrović, T., Stepanović, P., Bogunović, D., Gajić, B., Kulišić, Z., Ristić, M., Hadži-Milić, M.,& Dimitrijević, S.. (2020). Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia. in Arhiv veterinarske medicine
Novi Sad : Scientific Institute for Veterinary Medicine., 13(1), 5-28.
https://doi.org/10.46784/e-avm.v13i1.238

Ilić T, Petrović T, Stepanović P, Bogunović D, Gajić B, Kulišić Z, Ristić M, Hadži-Milić M, Dimitrijević S. Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia. in Arhiv veterinarske medicine. 2020;13(1):5-28.
doi:10.46784/e-avm.v13i1.238 .

Ilić, Tamara, Petrović, Tamaš, Stepanović, Predrag, Bogunović, Danica, Gajić, Bojan, Kulišić, Zoran, Ristić, Marko, Hadži-Milić, Milan, Dimitrijević, Sanda, "Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia" in Arhiv veterinarske medicine, 13, no. 1 (2020):5-28,
https://doi.org/10.46784/e-avm.v13i1.238 . .

TY  - JOUR
AU  - Aleksić, Jelena
AU  - Stepanović, Predrag
AU  - Dimitrijević, Sanda
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Davidov, Ivana
AU  - Aleksić-Agelidis, Aleksandra
AU  - Ilić, Tamara
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1971
AB  - Purpose: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. Methods: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. Results: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. Discussion: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. Conclusion: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Acta Parasitologica
T1  - Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores
VL  - 65
IS  - 4
SP  - 954
EP  - 962
DO  - 10.1007/s11686-020-00244-9
ER  - 

@article{
author = "Aleksić, Jelena and Stepanović, Predrag and Dimitrijević, Sanda and Gajić, Bojan and Bogunović, Danica and Davidov, Ivana and Aleksić-Agelidis, Aleksandra and Ilić, Tamara",
year = "2020",
abstract = "Purpose: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. Methods: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. Results: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. Discussion: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. Conclusion: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Acta Parasitologica",
title = "Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores",
volume = "65",
number = "4",
pages = "954-962",
doi = "10.1007/s11686-020-00244-9"
}

Aleksić, J., Stepanović, P., Dimitrijević, S., Gajić, B., Bogunović, D., Davidov, I., Aleksić-Agelidis, A.,& Ilić, T.. (2020). Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores. in Acta Parasitologica
Springer Science and Business Media Deutschland GmbH., 65(4), 954-962.
https://doi.org/10.1007/s11686-020-00244-9

Aleksić J, Stepanović P, Dimitrijević S, Gajić B, Bogunović D, Davidov I, Aleksić-Agelidis A, Ilić T. Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores. in Acta Parasitologica. 2020;65(4):954-962.
doi:10.1007/s11686-020-00244-9 .

Aleksić, Jelena, Stepanović, Predrag, Dimitrijević, Sanda, Gajić, Bojan, Bogunović, Danica, Davidov, Ivana, Aleksić-Agelidis, Aleksandra, Ilić, Tamara, "Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores" in Acta Parasitologica, 65, no. 4 (2020):954-962,
https://doi.org/10.1007/s11686-020-00244-9 . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Kulišić, Zoran
AU  - Despotović, Darko
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Dimitrijević, Sanda
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2178
AB  - Kontrola gastrointestinalnih parazita u različitim sistemima uzgoja preživara zasnivana je na vakcinaciji, hemoterapiji, poboljšanju kvaliteta menadžmenta stada i korišćenju genetskih potencijala domaćina. Širom sveta dominira strategija kontrole helminata zasnovana na čestoj upotrebi antihelmintika, koja se smatra neodrživom, s obzirom na pojavu sve većeg broja i vrsta parazita koji su otporni na lekove. Razvoj rezistencije na sve tri grupe antihelmintika širokog spektra (nikotinski antihelmintici, benzimidazoli i makrolidni laktoni) i povećana briga za zdravlje potrošača uslovljene pojavom rezidua primijenjenih lijekova u hrani, dodatno komplikuju kontrolu ovih nematoda. U cilju smanjenja brzine pojave rezistencije na antihelmintike kod gastrointestinalnih nematoda malih preživara i organizacije procesa odživog integrisanog upravljanja parazitima, globalno je prihvaćen princip ciljanog selektivnog tretmana. Sprovođenje ove strategije je tek odnedavno postalo izvodljivo, sa razvojem i praktičnom primenom sistema koji služi za kliničku procjenu anemije kod malih preživara obolelih od hemonhoze. Pored toga, kratkotrajne promene telesne težine i određivanje telesne kondicije mogu biti pokazatelji endoparazitoza, što omogućava brzu identifikaciju životinja koje će verovatno imati koristi od tretmana. Dobijeni rezultati kvantitativne koprološke dijagnostike i rezultati procene anemije su kriterijumi koji omogućavaju diferencijalnu dijagnostiku između zdravih i rezilijentnih životinja i lakšu dijagnostiku rezistencije parazita. S obzirom na to da rezilijentne životinje igraju važnu ulogu u kontaminaciji pašnjaka, razumljiv je kliničko-parazitološki značaj njihovog pravovremenog otkrivanja.
AB  - Control of the gastrointestinal parasites in different systems of ruminant breeding is based on vaccination, chemotherapy, improved herd management and use of genetic potentials of host animals. Strategy of the helminths control based on frequent anthelmintics usage is dominant among the world, although it is considered unsustainable due to the appearance of increased number and species of parasites that are resistance to drugs. Development of resistance on all three groups of broad-spectrum anthelmintics (nicotinic anthelmintics, benzimidazoles and macrolides lactones) as well as increased care for consumers health caused by appearance of used drugs residues in food, additionally complicate overall nematode control. With the aim to decrease appearance of anthelmintics resistance of gastrointestinal nematodes of small ruminants and in order to organize process of sustainable integrated parasite management, principle of targeted selective treatment is globally accepted. Implementing of this strategy has only recently become feasible, with development and practical use of systems that serve for clinical assessment of anemia in small ruminants which suffer from hemonchosis. Besides that, short term changes in body weight and body condition scoring may be indicators of diseases caused by endoparasites, as it can provide rapid identification of aminals that will probably have benefits from therapy. Obtained results of quantitative coprological diagnostic tests and results for anemia assessment are criteria that provide differential diagnosis between healthy and resilient animals and easier diseases diagnostic. Since resilient animals play important role in pasture contamination, the significance of their detection in understandable.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara
T1  - The importance of differntial diagnosis of resistance and resilience in ruminants
VL  - 19
IS  - 2
SP  - 228
EP  - 250
DO  - 10.7251/VETJSR1902228I
ER  - 

@article{
author = "Ilić, Tamara and Kulišić, Zoran and Despotović, Darko and Gajić, Bojan and Bogunović, Danica and Dimitrijević, Sanda",
year = "2019",
abstract = "Kontrola gastrointestinalnih parazita u različitim sistemima uzgoja preživara zasnivana je na vakcinaciji, hemoterapiji, poboljšanju kvaliteta menadžmenta stada i korišćenju genetskih potencijala domaćina. Širom sveta dominira strategija kontrole helminata zasnovana na čestoj upotrebi antihelmintika, koja se smatra neodrživom, s obzirom na pojavu sve većeg broja i vrsta parazita koji su otporni na lekove. Razvoj rezistencije na sve tri grupe antihelmintika širokog spektra (nikotinski antihelmintici, benzimidazoli i makrolidni laktoni) i povećana briga za zdravlje potrošača uslovljene pojavom rezidua primijenjenih lijekova u hrani, dodatno komplikuju kontrolu ovih nematoda. U cilju smanjenja brzine pojave rezistencije na antihelmintike kod gastrointestinalnih nematoda malih preživara i organizacije procesa odživog integrisanog upravljanja parazitima, globalno je prihvaćen princip ciljanog selektivnog tretmana. Sprovođenje ove strategije je tek odnedavno postalo izvodljivo, sa razvojem i praktičnom primenom sistema koji služi za kliničku procjenu anemije kod malih preživara obolelih od hemonhoze. Pored toga, kratkotrajne promene telesne težine i određivanje telesne kondicije mogu biti pokazatelji endoparazitoza, što omogućava brzu identifikaciju životinja koje će verovatno imati koristi od tretmana. Dobijeni rezultati kvantitativne koprološke dijagnostike i rezultati procene anemije su kriterijumi koji omogućavaju diferencijalnu dijagnostiku između zdravih i rezilijentnih životinja i lakšu dijagnostiku rezistencije parazita. S obzirom na to da rezilijentne životinje igraju važnu ulogu u kontaminaciji pašnjaka, razumljiv je kliničko-parazitološki značaj njihovog pravovremenog otkrivanja., Control of the gastrointestinal parasites in different systems of ruminant breeding is based on vaccination, chemotherapy, improved herd management and use of genetic potentials of host animals. Strategy of the helminths control based on frequent anthelmintics usage is dominant among the world, although it is considered unsustainable due to the appearance of increased number and species of parasites that are resistance to drugs. Development of resistance on all three groups of broad-spectrum anthelmintics (nicotinic anthelmintics, benzimidazoles and macrolides lactones) as well as increased care for consumers health caused by appearance of used drugs residues in food, additionally complicate overall nematode control. With the aim to decrease appearance of anthelmintics resistance of gastrointestinal nematodes of small ruminants and in order to organize process of sustainable integrated parasite management, principle of targeted selective treatment is globally accepted. Implementing of this strategy has only recently become feasible, with development and practical use of systems that serve for clinical assessment of anemia in small ruminants which suffer from hemonchosis. Besides that, short term changes in body weight and body condition scoring may be indicators of diseases caused by endoparasites, as it can provide rapid identification of aminals that will probably have benefits from therapy. Obtained results of quantitative coprological diagnostic tests and results for anemia assessment are criteria that provide differential diagnosis between healthy and resilient animals and easier diseases diagnostic. Since resilient animals play important role in pasture contamination, the significance of their detection in understandable.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara, The importance of differntial diagnosis of resistance and resilience in ruminants",
volume = "19",
number = "2",
pages = "228-250",
doi = "10.7251/VETJSR1902228I"
}

Ilić, T., Kulišić, Z., Despotović, D., Gajić, B., Bogunović, D.,& Dimitrijević, S.. (2019). Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 19(2), 228-250.
https://doi.org/10.7251/VETJSR1902228I

Ilić T, Kulišić Z, Despotović D, Gajić B, Bogunović D, Dimitrijević S. Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara. in Veterinarski žurnal Republike Srpske. 2019;19(2):228-250.
doi:10.7251/VETJSR1902228I .

Ilić, Tamara, Kulišić, Zoran, Despotović, Darko, Gajić, Bojan, Bogunović, Danica, Dimitrijević, Sanda, "Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara" in Veterinarski žurnal Republike Srpske, 19, no. 2 (2019):228-250,
https://doi.org/10.7251/VETJSR1902228I . .

TY  - JOUR
AU  - Vasić, Ana
AU  - Zdravković, Nemanja
AU  - Anita, Dragos
AU  - Bojkovski, Jovan
AU  - Marinov, Mihai
AU  - Mathis, Alexander
AU  - Niculaua, Marius
AU  - Oslobanu, Elena Luanda
AU  - Pavlović, Ivan
AU  - Petrić, Dusan
AU  - Pflueger, Valentin
AU  - Pudar, Dubravka
AU  - Savuta, Gheorghe
AU  - Simeunović, Predrag
AU  - Veronesi, Eva
AU  - Silaghi, Cornelia
AU  - Anita, Adriana
AU  - Anton, Ioana Alexandra
AU  - Cimpan, Andrei
AU  - Ciuca, Lavinia
AU  - Crivei, Luciana
AU  - Cojkić, Aleksandar
AU  - Davitkov, Darko
AU  - Drašković, Vladimir
AU  - Gajić, Bojan
AU  - Glavinić, Uroš
AU  - Ivanescu, Maria-Larisa
AU  - Kavran, Mihaela
AU  - Lupu, Andrei-Cristian
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Prodanović, Radiša
AU  - Radanović, Oliver
AU  - Raileanu, Cristian
AU  - Raileanu, Stefan
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Vaselek, Slavica
AU  - Đurić, Miloje
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1740
AB  - BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia
VL  - 12
SP  - 61
DO  - 10.1186/s13071-019-3292-3
ER  - 

@article{
author = "Vasić, Ana and Zdravković, Nemanja and Anita, Dragos and Bojkovski, Jovan and Marinov, Mihai and Mathis, Alexander and Niculaua, Marius and Oslobanu, Elena Luanda and Pavlović, Ivan and Petrić, Dusan and Pflueger, Valentin and Pudar, Dubravka and Savuta, Gheorghe and Simeunović, Predrag and Veronesi, Eva and Silaghi, Cornelia and Anita, Adriana and Anton, Ioana Alexandra and Cimpan, Andrei and Ciuca, Lavinia and Crivei, Luciana and Cojkić, Aleksandar and Davitkov, Darko and Drašković, Vladimir and Gajić, Bojan and Glavinić, Uroš and Ivanescu, Maria-Larisa and Kavran, Mihaela and Lupu, Andrei-Cristian and Mindru, Raluca and Porea, Daniela and Prodanović, Radiša and Radanović, Oliver and Raileanu, Cristian and Raileanu, Stefan and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Vaselek, Slavica and Đurić, Miloje",
year = "2019",
abstract = "BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia",
volume = "12",
pages = "61",
doi = "10.1186/s13071-019-3292-3"
}

Vasić, A., Zdravković, N., Anita, D., Bojkovski, J., Marinov, M., Mathis, A., Niculaua, M., Oslobanu, E. L., Pavlović, I., Petrić, D., Pflueger, V., Pudar, D., Savuta, G., Simeunović, P., Veronesi, E., Silaghi, C., Anita, A., Anton, I. A., Cimpan, A., Ciuca, L., Crivei, L., Cojkić, A., Davitkov, D., Drašković, V., Gajić, B., Glavinić, U., Ivanescu, M., Kavran, M., Lupu, A., Mindru, R., Porea, D., Prodanović, R., Radanović, O., Raileanu, C., Raileanu, S., Ristanić, M., Roman, C., Stanišić, L., Vaselek, S.,& Đurić, M.. (2019). Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors
BMC, London., 12, 61.
https://doi.org/10.1186/s13071-019-3292-3

Vasić A, Zdravković N, Anita D, Bojkovski J, Marinov M, Mathis A, Niculaua M, Oslobanu EL, Pavlović I, Petrić D, Pflueger V, Pudar D, Savuta G, Simeunović P, Veronesi E, Silaghi C, Anita A, Anton IA, Cimpan A, Ciuca L, Crivei L, Cojkić A, Davitkov D, Drašković V, Gajić B, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Prodanović R, Radanović O, Raileanu C, Raileanu S, Ristanić M, Roman C, Stanišić L, Vaselek S, Đurić M. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors. 2019;12:61.
doi:10.1186/s13071-019-3292-3 .

Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia" in Parasites & Vectors, 12 (2019):61,
https://doi.org/10.1186/s13071-019-3292-3 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Munoz, Irene
AU  - de la Rua, Pilar
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Kulišić, Zoran
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1787
AB  - The aim of this study was to investigate the genetic diversity of Varroa destructor parasitizing Apis mellifera colonies and to test for possible host-parasite association at the mitochondrial DNA (mtDNA) level. Six A. mellifera haplotypes (including a novel C2aa) and five haplotypes of V. destructor were detected in 29 analyzed colonies from eight sampling sites in Serbia. We revealed the presence of the K and S1 haplotypes as well as KS1 and KP1 heteroplasmic mite individuals in all localities, while the P1 haplotype was only found in four sampling sites. Significant differences in V. destructor genetic diversity were found at both apiary and colony levels, with mite haplotypes coexisting in almost all tested colonies. In addition, a significant correlation between the number of analyzed mites per colony and the number of identified V. destructor haplotypes was observed. However, no significant host-parasite relationship was found, suggesting that mites bearing different haplotypes as well as those heteroplasmic individuals are well adapted to the host, A. mellifera, independently of the identified haplotype present in each colony. Our results will contribute to future population and biogeographic studies concerning V. destructor infesting A. mellifera, as well as to better understanding their host-parasite relationship.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Coexistence of genetically different Varroa destructor in Apis mellifera colonies
VL  - 78
IS  - 3
SP  - 315
EP  - 326
DO  - 10.1007/s10493-019-00395-z
ER  - 

@article{
author = "Gajić, Bojan and Munoz, Irene and de la Rua, Pilar and Stevanović, Jevrosima and Lakić, Nada and Kulišić, Zoran and Stanimirović, Zoran",
year = "2019",
abstract = "The aim of this study was to investigate the genetic diversity of Varroa destructor parasitizing Apis mellifera colonies and to test for possible host-parasite association at the mitochondrial DNA (mtDNA) level. Six A. mellifera haplotypes (including a novel C2aa) and five haplotypes of V. destructor were detected in 29 analyzed colonies from eight sampling sites in Serbia. We revealed the presence of the K and S1 haplotypes as well as KS1 and KP1 heteroplasmic mite individuals in all localities, while the P1 haplotype was only found in four sampling sites. Significant differences in V. destructor genetic diversity were found at both apiary and colony levels, with mite haplotypes coexisting in almost all tested colonies. In addition, a significant correlation between the number of analyzed mites per colony and the number of identified V. destructor haplotypes was observed. However, no significant host-parasite relationship was found, suggesting that mites bearing different haplotypes as well as those heteroplasmic individuals are well adapted to the host, A. mellifera, independently of the identified haplotype present in each colony. Our results will contribute to future population and biogeographic studies concerning V. destructor infesting A. mellifera, as well as to better understanding their host-parasite relationship.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Coexistence of genetically different Varroa destructor in Apis mellifera colonies",
volume = "78",
number = "3",
pages = "315-326",
doi = "10.1007/s10493-019-00395-z"
}

Gajić, B., Munoz, I., de la Rua, P., Stevanović, J., Lakić, N., Kulišić, Z.,& Stanimirović, Z.. (2019). Coexistence of genetically different Varroa destructor in Apis mellifera colonies. in Experimental and Applied Acarology
Springer, Dordrecht., 78(3), 315-326.
https://doi.org/10.1007/s10493-019-00395-z

Gajić B, Munoz I, de la Rua P, Stevanović J, Lakić N, Kulišić Z, Stanimirović Z. Coexistence of genetically different Varroa destructor in Apis mellifera colonies. in Experimental and Applied Acarology. 2019;78(3):315-326.
doi:10.1007/s10493-019-00395-z .

Gajić, Bojan, Munoz, Irene, de la Rua, Pilar, Stevanović, Jevrosima, Lakić, Nada, Kulišić, Zoran, Stanimirović, Zoran, "Coexistence of genetically different Varroa destructor in Apis mellifera colonies" in Experimental and Applied Acarology, 78, no. 3 (2019):315-326,
https://doi.org/10.1007/s10493-019-00395-z . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Bugarski-Stanojević, Vanja
AU  - Penezić, Aleksandra
AU  - Kurucki, Milica
AU  - Bogdanović, Neda
AU  - Ćirović, Duško
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1768
AB  - Thelazia callipaeda, originally known as an Oriental eyeworm, is a small nematode parasitizing the conjunctival sacs of domestic and wild animals and humans. Previous studies conducted in Serbia have reported the eyeworm infections in dogs, cats, and foxes, as well as in a human patient. As the data regarding thelaziosis from wildlife is still scarce, the aim of this study was to investigate the presence of T. callipaeda in gray wolf (Canis lupus) from Serbia. All collected nematodes were morphologically identified as T. callipaeda males (n = 64) or females (n = 225). Molecular characterization, conducted by PCR amplification followed by sequence analysis of partial cytochrome c oxidase subunit 1 gene (cox1), revealed only haplotype 1 of T. callipaeda. The overall prevalence of thelaziosis was 38.1% (8/21). In all positive animals, both eyes were affected, with a total parasitic load ranging from four to 132 worms per animal. Our results indicate the important epidemiological role of wolves as wildlife reservoirs of T. callipaeda, expanding geographic range of infection, as well as intra- and interspecies contact rates, although the role of other wild carnivore species (i.e., foxes and jackals) should be investigated in future studies.
PB  - Springer, New York
T2  - Parasitology Research
T1  - First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia
VL  - 118
IS  - 12
SP  - 3549
EP  - 3553
DO  - 10.1007/s00436-019-06519-z
ER  - 

@article{
author = "Gajić, Bojan and Bugarski-Stanojević, Vanja and Penezić, Aleksandra and Kurucki, Milica and Bogdanović, Neda and Ćirović, Duško",
year = "2019",
abstract = "Thelazia callipaeda, originally known as an Oriental eyeworm, is a small nematode parasitizing the conjunctival sacs of domestic and wild animals and humans. Previous studies conducted in Serbia have reported the eyeworm infections in dogs, cats, and foxes, as well as in a human patient. As the data regarding thelaziosis from wildlife is still scarce, the aim of this study was to investigate the presence of T. callipaeda in gray wolf (Canis lupus) from Serbia. All collected nematodes were morphologically identified as T. callipaeda males (n = 64) or females (n = 225). Molecular characterization, conducted by PCR amplification followed by sequence analysis of partial cytochrome c oxidase subunit 1 gene (cox1), revealed only haplotype 1 of T. callipaeda. The overall prevalence of thelaziosis was 38.1% (8/21). In all positive animals, both eyes were affected, with a total parasitic load ranging from four to 132 worms per animal. Our results indicate the important epidemiological role of wolves as wildlife reservoirs of T. callipaeda, expanding geographic range of infection, as well as intra- and interspecies contact rates, although the role of other wild carnivore species (i.e., foxes and jackals) should be investigated in future studies.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia",
volume = "118",
number = "12",
pages = "3549-3553",
doi = "10.1007/s00436-019-06519-z"
}

Gajić, B., Bugarski-Stanojević, V., Penezić, A., Kurucki, M., Bogdanović, N.,& Ćirović, D.. (2019). First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia. in Parasitology Research
Springer, New York., 118(12), 3549-3553.
https://doi.org/10.1007/s00436-019-06519-z

Gajić B, Bugarski-Stanojević V, Penezić A, Kurucki M, Bogdanović N, Ćirović D. First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia. in Parasitology Research. 2019;118(12):3549-3553.
doi:10.1007/s00436-019-06519-z .

Gajić, Bojan, Bugarski-Stanojević, Vanja, Penezić, Aleksandra, Kurucki, Milica, Bogdanović, Neda, Ćirović, Duško, "First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia" in Parasitology Research, 118, no. 12 (2019):3549-3553,
https://doi.org/10.1007/s00436-019-06519-z . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Becskei, Zsolt
AU  - Gajić, Bojan
AU  - Ozvegy, Jozsef
AU  - Stepanović, Predrag
AU  - Nenadović, Katarina
AU  - Dimitrijević, Sanda
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1646
AB  - This report represents the prevalence of endoparasitic infections of birds in zoological gardens in Serbia. Coprological examination conducted during 2015 and 2016, involved samples from 318 individual birds belonging to 112 species, in three zoo gardens (Belgrade, Palic and Bor). Individual faeces samples were analysed by the flotation method in salt solution. The presence of endoparasites was established in 51.96% (Belgrade), 46.16% (Palic) and 16.66% (Bor) of the tested birds. We diagnosed coccidian oocysts, eggs of Capillaria spp, Heterakis spp, Trichostrongylus spp, Ascaridia spp, Syngamus trachea and eggs of yet unidentified trematode. There were no detection of parasites from the class of cestodes. Prevalence of infection with coccidia, depending on location, was in the ranges between 8.33% and 32.84%, capillariosis 2.77% and 29.41%, heterakiosis 2.56% and 12.74%, trichostrongylidosis 5.55% and 25.64%, askaridiosis 10.25% and 10.78%, syngamosis 2.94% and trematodes of unknown etiology 2.45%. Coinfections with two or three parasitic species were detected in Belgrade and Palic Zoo Gardens, while quadruple infection was established in birds of Belgrade Zoo Garden. Obtained results can be used in establishing programs for health protection of endangered bird species.
PB  - Springer International Publishing Ag, Cham
T2  - Acta Parasitologica
T1  - Prevalence of endoparasitic infections of birds in zoo gardens in Serbia
VL  - 63
IS  - 1
SP  - 134
EP  - 146
DO  - 10.1515/ap-2018-0015
ER  - 

@article{
author = "Ilić, Tamara and Becskei, Zsolt and Gajić, Bojan and Ozvegy, Jozsef and Stepanović, Predrag and Nenadović, Katarina and Dimitrijević, Sanda",
year = "2018",
abstract = "This report represents the prevalence of endoparasitic infections of birds in zoological gardens in Serbia. Coprological examination conducted during 2015 and 2016, involved samples from 318 individual birds belonging to 112 species, in three zoo gardens (Belgrade, Palic and Bor). Individual faeces samples were analysed by the flotation method in salt solution. The presence of endoparasites was established in 51.96% (Belgrade), 46.16% (Palic) and 16.66% (Bor) of the tested birds. We diagnosed coccidian oocysts, eggs of Capillaria spp, Heterakis spp, Trichostrongylus spp, Ascaridia spp, Syngamus trachea and eggs of yet unidentified trematode. There were no detection of parasites from the class of cestodes. Prevalence of infection with coccidia, depending on location, was in the ranges between 8.33% and 32.84%, capillariosis 2.77% and 29.41%, heterakiosis 2.56% and 12.74%, trichostrongylidosis 5.55% and 25.64%, askaridiosis 10.25% and 10.78%, syngamosis 2.94% and trematodes of unknown etiology 2.45%. Coinfections with two or three parasitic species were detected in Belgrade and Palic Zoo Gardens, while quadruple infection was established in birds of Belgrade Zoo Garden. Obtained results can be used in establishing programs for health protection of endangered bird species.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Acta Parasitologica",
title = "Prevalence of endoparasitic infections of birds in zoo gardens in Serbia",
volume = "63",
number = "1",
pages = "134-146",
doi = "10.1515/ap-2018-0015"
}

Ilić, T., Becskei, Z., Gajić, B., Ozvegy, J., Stepanović, P., Nenadović, K.,& Dimitrijević, S.. (2018). Prevalence of endoparasitic infections of birds in zoo gardens in Serbia. in Acta Parasitologica
Springer International Publishing Ag, Cham., 63(1), 134-146.
https://doi.org/10.1515/ap-2018-0015

Ilić T, Becskei Z, Gajić B, Ozvegy J, Stepanović P, Nenadović K, Dimitrijević S. Prevalence of endoparasitic infections of birds in zoo gardens in Serbia. in Acta Parasitologica. 2018;63(1):134-146.
doi:10.1515/ap-2018-0015 .

Ilić, Tamara, Becskei, Zsolt, Gajić, Bojan, Ozvegy, Jozsef, Stepanović, Predrag, Nenadović, Katarina, Dimitrijević, Sanda, "Prevalence of endoparasitic infections of birds in zoo gardens in Serbia" in Acta Parasitologica, 63, no. 1 (2018):134-146,
https://doi.org/10.1515/ap-2018-0015 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Vejnović, Branislav
AU  - Mirilović, Milorad
AU  - Kulišić, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2129
AB  - Bolesti pasa koje se prenose vektorima predstavljaju oboljenja virusne,
bakterijske ili parazitske etiologije, koja se najčešće prenose hematofagnim
artropodama, komarcima i krpeljima.
Cilj ovog rada bio je da se ispita prisustvo infekcija čiji se uzročnici prenose
komarcima (Dirofilaria immitis) i krpeljima (Borrelia burgdorferi, Anaplasma
phagocytophilum/A. platys, Ehrlichia canis/E. ewingii) kod nevlasničkih pasa na
teritoriji Beograda.
U periodu od septembra do decembra 2015. godine sakupljeno je 86 uzoraka krvi
od klinički zdravih nevlasničkih pasa iz različitih beogradskih opština. Svi uzorci
testirani su modinikovanim Knott-ovim testom i komercijalnim SNAP® 4Dx Plus®
Test-om.
Infekcija izazvana barem jednim od ispitivanih uzročnika ustanovljena je kod
27,91% pasa. Najzastupljeniji uzročnik bila je D. immitis, koja je dijagnostikovana
kod 17,44% životinja. Na prisustvo antitela protiv Anaplasma spp. pozitivno je bilo
9,30% pasa, dok je 2,33% jedinki bilo seropozitivno na B. burgdorferi, odnosno na
Ehrlichia spp. Koinfekcije sa dva patogena zabeležene su kod 3,49% pasa.
Rezultati ovog istraživanja pokazuju da su nevlasnički psi na teritoriji grada
Beograda izloženi delovanju različitih vrsta zoonoznih uzročnika koji se prenose
komarcima i krpeljima, zbog čega treba preduzeti odgovarajuće mere za suzbijanje
ovih artropoda.
AB  - Vector-borne diseases are caused by viruses, bacteria or parasites transmitted by
hematophagous arthropods, mainly mosquitoes and ticks.
The aim of this work was to study the presence of pathogens vectored by
mosquitoes (DiroGilaria immitis) and ticks (Borrelia burgdorferi, Anaplasma
phagocytophilum/A. platys, Ehrlichia canis/E. ewingii) in stray dogs from Belgrade.
From September to December 2015, 86 blood samples were collected from
apparently healthy stray dogs in different Belgrade municipalities. All samples were
analysed using modiNied Knott’s test and commercial SNAP® 4Dx Plus®Test.
Infection with at least one pathogen was detected in 27,91% of investigated dogs.
The most prevalent agent was D. immitis that was diagnosed in 17,44% of animals.
Antibodies against Anaplasma spp. were found in 9,30% of dogs and 2,33%
individuals were seropositive to B. burgdorferi, as well as to Ehrlichia spp. Coinfections
with two pathogens were recorded in 3,49% of dogs.
Results of this research show that stray dogs in Belgrade are exposed to different
zoonotic pathogens transmitted by mosquitoes and ticks, indicating the need for
appropriate control against these arthropod species.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Zastupljenost Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. i Ehrlichia spp. kod nevlasničkih pasa na području Beograda
T1  - Prevalence of Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. and Ehrlichia spp. in stray dogs in Belgrade area
VL  - 16
IS  - 2
SP  - 142
EP  - 151
DO  - 10.7251/VETJ1602002G
ER  - 

@article{
author = "Gajić, Bojan and Bogunović, Danica and Vejnović, Branislav and Mirilović, Milorad and Kulišić, Zoran",
year = "2016",
abstract = "Bolesti pasa koje se prenose vektorima predstavljaju oboljenja virusne,
bakterijske ili parazitske etiologije, koja se najčešće prenose hematofagnim
artropodama, komarcima i krpeljima.
Cilj ovog rada bio je da se ispita prisustvo infekcija čiji se uzročnici prenose
komarcima (Dirofilaria immitis) i krpeljima (Borrelia burgdorferi, Anaplasma
phagocytophilum/A. platys, Ehrlichia canis/E. ewingii) kod nevlasničkih pasa na
teritoriji Beograda.
U periodu od septembra do decembra 2015. godine sakupljeno je 86 uzoraka krvi
od klinički zdravih nevlasničkih pasa iz različitih beogradskih opština. Svi uzorci
testirani su modinikovanim Knott-ovim testom i komercijalnim SNAP® 4Dx Plus®
Test-om.
Infekcija izazvana barem jednim od ispitivanih uzročnika ustanovljena je kod
27,91% pasa. Najzastupljeniji uzročnik bila je D. immitis, koja je dijagnostikovana
kod 17,44% životinja. Na prisustvo antitela protiv Anaplasma spp. pozitivno je bilo
9,30% pasa, dok je 2,33% jedinki bilo seropozitivno na B. burgdorferi, odnosno na
Ehrlichia spp. Koinfekcije sa dva patogena zabeležene su kod 3,49% pasa.
Rezultati ovog istraživanja pokazuju da su nevlasnički psi na teritoriji grada
Beograda izloženi delovanju različitih vrsta zoonoznih uzročnika koji se prenose
komarcima i krpeljima, zbog čega treba preduzeti odgovarajuće mere za suzbijanje
ovih artropoda., Vector-borne diseases are caused by viruses, bacteria or parasites transmitted by
hematophagous arthropods, mainly mosquitoes and ticks.
The aim of this work was to study the presence of pathogens vectored by
mosquitoes (DiroGilaria immitis) and ticks (Borrelia burgdorferi, Anaplasma
phagocytophilum/A. platys, Ehrlichia canis/E. ewingii) in stray dogs from Belgrade.
From September to December 2015, 86 blood samples were collected from
apparently healthy stray dogs in different Belgrade municipalities. All samples were
analysed using modiNied Knott’s test and commercial SNAP® 4Dx Plus®Test.
Infection with at least one pathogen was detected in 27,91% of investigated dogs.
The most prevalent agent was D. immitis that was diagnosed in 17,44% of animals.
Antibodies against Anaplasma spp. were found in 9,30% of dogs and 2,33%
individuals were seropositive to B. burgdorferi, as well as to Ehrlichia spp. Coinfections
with two pathogens were recorded in 3,49% of dogs.
Results of this research show that stray dogs in Belgrade are exposed to different
zoonotic pathogens transmitted by mosquitoes and ticks, indicating the need for
appropriate control against these arthropod species.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Zastupljenost Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. i Ehrlichia spp. kod nevlasničkih pasa na području Beograda, Prevalence of Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. and Ehrlichia spp. in stray dogs in Belgrade area",
volume = "16",
number = "2",
pages = "142-151",
doi = "10.7251/VETJ1602002G"
}

Gajić, B., Bogunović, D., Vejnović, B., Mirilović, M.,& Kulišić, Z.. (2016). Zastupljenost Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. i Ehrlichia spp. kod nevlasničkih pasa na području Beograda. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 16(2), 142-151.
https://doi.org/10.7251/VETJ1602002G

Gajić B, Bogunović D, Vejnović B, Mirilović M, Kulišić Z. Zastupljenost Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. i Ehrlichia spp. kod nevlasničkih pasa na području Beograda. in Veterinarski žurnal Republike Srpske. 2016;16(2):142-151.
doi:10.7251/VETJ1602002G .

Gajić, Bojan, Bogunović, Danica, Vejnović, Branislav, Mirilović, Milorad, Kulišić, Zoran, "Zastupljenost Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp. i Ehrlichia spp. kod nevlasničkih pasa na području Beograda" in Veterinarski žurnal Republike Srpske, 16, no. 2 (2016):142-151,
https://doi.org/10.7251/VETJ1602002G . .

TY  - JOUR
AU  - Davitkov, Darko
AU  - Terzić, Srećko
AU  - Davitkov, Dajana
AU  - Radaković, Milena
AU  - Gajić, Bojan
AU  - Krstić, Vanja
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1348
AB  - Babesiosis of domestic animals is a vector transmissible and clinically significant disease, caused by protozoa of genus Babesia and Theileria. Possible causative agents for this disease in dogs in Europe are: Babesia canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis of dogs was for a long time based on the visual inspection of stained blood smear under a microscope, while today there have been increasingly used molecular methods of detection in precise, species diagnostics. The objective of this work was molecular detection of the cause of babesiosis of dogs in the ticks sampled from asymptomatic dogs in the region of some Belgrade municipalities, all for better understanding of epizootiological situation. From three sites in Belgrade, there were collected 49 ticks, sampled from the dogs with no symptoms. There was carried out the determination of the ticks, and after that, DNA was isolated for molecular examination. First, theret was performed Polymerase Chain Reaction (PCR), for determining the species of the genus Babesia, and after that there was also carried out the determining of polymorphism in the length of restriction fragments (RFLP) for the purpose of the causative agent species determination. Out of the total number of the examined ticks, 18,34% were positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni was identified, while in 7 cases (14,92%) there were no restriction sites for the used enzymes, what suggests that most likely it was B. canis. The ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4 cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This work confirms the presence of Babesia spp. in the ticks sampled from asmptomatic dogs on the territory of Belgrade as well as the significance of PCR-RFLP method in diagnostics and identification of the causative agent of babesiosis in dogs. For the first time in Serbia, there was determined the presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus).
AB  - Babezioza domaćih životinja predstavlja vektorski prenosivo i klinički veoma značajno oboljenje, uzrokovano protozoama rodova Babesia i Theileria. Uzročnici koji mogu da izazovu oboljenje kod pasa u Evropi su: Babesia canis, B. gibsoni, B. vogeli i B. microti-like. Dijagnostika babezioze pasa se dugo vremena bazirala na vizuelizaciji uzročnika pregledom obojenog krvnog razmaza pod mikroskopom, dok se danas sve više koriste molekularne metode detekcije u preciznoj, specijskoj dijagnostici. Cilj ovog rada je bila molekularna detekcija različitih uzročnika babezioze pasa u krpeljima uzorkovanim sa asimptomatskih pasa na prostoru određenih beogradskih opština, radi boljeg razumevanja epizootiološke situacije. Sa tri lokacije u Beogradu je prikupljeno 49 krpelja, uzorkovanih sa pasa bez simptoma bolesti. Izvršena je determinacja krpelja, a nakon toga je izolovana DNK za molekularna ispitivanja. Prvo je urađena reakcija lančane polimerizacije (PCR) za utvrđivanje vrsta iz roda Babesia, a nakon toga i određivanje polimorfizma u dužini restrikcionih fragmenata (RFLP) u cilju specijske identifikacije uzročnika. Od ukupnog broja ispitanih krpelja 18,34% je bilo pozitivno na Babesia spp. RFLP metodom je u dva slučaja (4,08%) identifikovana B. gibsoni, dok u 7 slučajeva (14,92%) nije bilo restrkicionih mesta za korišćene enzime, što ukazuje da se najverovatnije radilo o B. canis. Pozitivni krpelji na uzročnike babezioze su bili Dermacentor reticulatus (4 slučaja), Rhipicephalus sanguineus (4 slučaja) i Ixodes ricinus (1 slučaj). Ovaj rad potvrđuje prisustvo Babesia spp. u krpeljima uzorkovanim sa asimptomatskih pasa na teritoriji grada Beograda, kao i značaj PCR-RFLP metode u dijagnostici i identifikaciji uzročnika babezioze pasa. Prvi put u Srbiji je utvrđeno prisustvo B. gibsoni kod krpelja (vrsta Rhipicephalus sanguineus).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities
T1  - Molekuljarnaja detekcija Babesia spp. v obrazcah kleščej, polučennyh ot asimptomatičnyh sobak na territorii nekotoryh rajonov Belgrada
T1  - Molekularna detekcija Babesia spp. u krpeljima uzorkovanim sa asimptomatskih pasa na području određenih beogradskih opština
VL  - 70
IS  - 5-6
SP  - 175
EP  - 184
DO  - 10.2298/VETGL1606175D
ER  - 

@article{
author = "Davitkov, Darko and Terzić, Srećko and Davitkov, Dajana and Radaković, Milena and Gajić, Bojan and Krstić, Vanja and Stanimirović, Zoran",
year = "2016",
abstract = "Babesiosis of domestic animals is a vector transmissible and clinically significant disease, caused by protozoa of genus Babesia and Theileria. Possible causative agents for this disease in dogs in Europe are: Babesia canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis of dogs was for a long time based on the visual inspection of stained blood smear under a microscope, while today there have been increasingly used molecular methods of detection in precise, species diagnostics. The objective of this work was molecular detection of the cause of babesiosis of dogs in the ticks sampled from asymptomatic dogs in the region of some Belgrade municipalities, all for better understanding of epizootiological situation. From three sites in Belgrade, there were collected 49 ticks, sampled from the dogs with no symptoms. There was carried out the determination of the ticks, and after that, DNA was isolated for molecular examination. First, theret was performed Polymerase Chain Reaction (PCR), for determining the species of the genus Babesia, and after that there was also carried out the determining of polymorphism in the length of restriction fragments (RFLP) for the purpose of the causative agent species determination. Out of the total number of the examined ticks, 18,34% were positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni was identified, while in 7 cases (14,92%) there were no restriction sites for the used enzymes, what suggests that most likely it was B. canis. The ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4 cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This work confirms the presence of Babesia spp. in the ticks sampled from asmptomatic dogs on the territory of Belgrade as well as the significance of PCR-RFLP method in diagnostics and identification of the causative agent of babesiosis in dogs. For the first time in Serbia, there was determined the presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus)., Babezioza domaćih životinja predstavlja vektorski prenosivo i klinički veoma značajno oboljenje, uzrokovano protozoama rodova Babesia i Theileria. Uzročnici koji mogu da izazovu oboljenje kod pasa u Evropi su: Babesia canis, B. gibsoni, B. vogeli i B. microti-like. Dijagnostika babezioze pasa se dugo vremena bazirala na vizuelizaciji uzročnika pregledom obojenog krvnog razmaza pod mikroskopom, dok se danas sve više koriste molekularne metode detekcije u preciznoj, specijskoj dijagnostici. Cilj ovog rada je bila molekularna detekcija različitih uzročnika babezioze pasa u krpeljima uzorkovanim sa asimptomatskih pasa na prostoru određenih beogradskih opština, radi boljeg razumevanja epizootiološke situacije. Sa tri lokacije u Beogradu je prikupljeno 49 krpelja, uzorkovanih sa pasa bez simptoma bolesti. Izvršena je determinacja krpelja, a nakon toga je izolovana DNK za molekularna ispitivanja. Prvo je urađena reakcija lančane polimerizacije (PCR) za utvrđivanje vrsta iz roda Babesia, a nakon toga i određivanje polimorfizma u dužini restrikcionih fragmenata (RFLP) u cilju specijske identifikacije uzročnika. Od ukupnog broja ispitanih krpelja 18,34% je bilo pozitivno na Babesia spp. RFLP metodom je u dva slučaja (4,08%) identifikovana B. gibsoni, dok u 7 slučajeva (14,92%) nije bilo restrkicionih mesta za korišćene enzime, što ukazuje da se najverovatnije radilo o B. canis. Pozitivni krpelji na uzročnike babezioze su bili Dermacentor reticulatus (4 slučaja), Rhipicephalus sanguineus (4 slučaja) i Ixodes ricinus (1 slučaj). Ovaj rad potvrđuje prisustvo Babesia spp. u krpeljima uzorkovanim sa asimptomatskih pasa na teritoriji grada Beograda, kao i značaj PCR-RFLP metode u dijagnostici i identifikaciji uzročnika babezioze pasa. Prvi put u Srbiji je utvrđeno prisustvo B. gibsoni kod krpelja (vrsta Rhipicephalus sanguineus).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities, Molekuljarnaja detekcija Babesia spp. v obrazcah kleščej, polučennyh ot asimptomatičnyh sobak na territorii nekotoryh rajonov Belgrada, Molekularna detekcija Babesia spp. u krpeljima uzorkovanim sa asimptomatskih pasa na području određenih beogradskih opština",
volume = "70",
number = "5-6",
pages = "175-184",
doi = "10.2298/VETGL1606175D"
}

Davitkov, D., Terzić, S., Davitkov, D., Radaković, M., Gajić, B., Krstić, V.,& Stanimirović, Z.. (2016). Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(5-6), 175-184.
https://doi.org/10.2298/VETGL1606175D

Davitkov D, Terzić S, Davitkov D, Radaković M, Gajić B, Krstić V, Stanimirović Z. Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities. in Veterinarski Glasnik. 2016;70(5-6):175-184.
doi:10.2298/VETGL1606175D .

Davitkov, Darko, Terzić, Srećko, Davitkov, Dajana, Radaković, Milena, Gajić, Bojan, Krstić, Vanja, Stanimirović, Zoran, "Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities" in Veterinarski Glasnik, 70, no. 5-6 (2016):175-184,
https://doi.org/10.2298/VETGL1606175D . .

TY  - THES
AU  - Gajić, Bojan
PY  - 2016
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48025103
UR  - http://nardus.mpn.gov.rs/123456789/5873
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/34
AB  - In this study, genetic variability of honey bee Apis mellifera and honey bee mite Varroa destructor populations was analyzed, monitoring haplotype-based distribution pattern between the host and the parasite. For the identification of honey bee haplotypes, samples from 48 honey bee colonies were used, while 245 Varroa mite samples originating from 29 colonies were analyzed for haplotype identification and monitoring correlation between A. mellifera and V. destructor haplotypes. Sequencing of tRNAleu-cox2 region revealed six A. mellifera haplotypes, with C2aa haplotype observed for the first time. Different frequency of identifed bee haplotypes was detected in total sample, with C2d haplotype being the most frequent, and C2aa and C2i haplotypes with the lowest frequency. Distribution of A. mellifera haplotypes was found to be dependent on the locality, with three sites represented by only one haplotype, one locality represented by two haplotypes and four localities represented by three honey bee haplotypes. Sequencing results of V. destructor mitochondrial fragments revealed the point mutations at position 1932 and 10133 mtDNA within cox1 and cytb sequences, respectively. Based on these polymorphic sites, two novel Varroa haplotypes were defined and named as Serbia 1 (S1) and Peshter 1 (P1). Both detected mutations were the silent ones and did not affect the protein sequence. At the sequence chromatograms in some of the analyzed samples, double peaks were detected at haplotype-defining sites within cox1 and cytb, observing the phenomenon of nucleotide heteroplasmy for the first time in V. destructor. After the polymorphic sites had been observed using the sequencing method, ARMS and RFLP assays were performed for identification of Varroa haplotypes and detection of heteroplasmic individuals. Results of the cox1 sequence analysis showed that 48,2% of individuals belonged to the K haplotype, 31,8% belonged to the S1 haplotype and 20,0% Varroa samples was found to be heteroplasmic...
AB  - U ovom radu ispitivana je genetička varijabilnost medonosne pčele Apis mellifera i pčelinjeg krpelja Varroa destructor, uz praćenje korelacije u distribuciji haplotipova domaćina i parazita. Za identifikaciju haplotipova pčela korišćeni su uzorci iz 48 pčelinjih društava sa osam lokaliteta na teritoriji Srbije, dok je za tipizaciju varoa i praćenje korelacije haplotipova domaćina i parazita analizirano 245 uzoraka varoe iz 29 košnica. Sekvencioniranjem tRNAleu-cox2 regiona definisano je šest haplotipova A. mellifera, pri čemu je haplotip C2aa opisan po prvi put. Zastupljenost identifikovanih haplotipova pčela razlikovala se u ukupnom uzorku, pri čemu je najzastupljeniji bio haplotip C2d, dok su najmanje prisutni bili haplotipovi C2aa i C2i. Distribucija haplotipova A. mellifera razlikovala se u zavisnosti od lokaliteta, pri čemu je na tri posmatrana lokaliteta utvrđen po jedan haplotip, na jednom lokalitetu dva i na četiri lokaliteta utvrđeno je prisustvo tri haplotipa pčela. Rezultati sekvencioniranja delova mitohondrijalnih gena V. destructor otkrili su prisustvo tačkastih mutacija na pozicijama 1932 i 10133 mtDNK unutar cox1 i cytb sekvenci. Na osnovu tih polimorfnih mesta, definisana su dva nova haplotipa varoe koji su nazvani Srbija 1 (S1) i Pešter 1 (P1). Obe otkrivene mutacije po svojoj prirodi spadaju u „tihe‟ mutacije koje ne dovode do promene proteinskog lanca. Na hromatogramima pojedinih uzoraka otkriveni su dupli pikovi na haplotip definišućim mestima unutar cox1 i cytb, čime je po prvi put utvrđena nukleotidna heteroplazmija kod vrste V. destructor. Nakon utvrđivanja polimorfnih mesta metodom sekvencioniranja, za identifikaciju haplotipova varoe i otkrivanje jedinki sa heteroplazmijom korišćene su ARMS i RFLP metode. Analizom cox1 sekvence ARMS metodom utvrđeno je 48,2% jedinki K haplotipa, 31,8% jedinki S1 haplotipa i 20,0% jedinki sa heteroplazmijom...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Analysis of genetic variability and haplotype correlation between honey bee Apis mellifera and honey bee mite Varroa destructor
T1  - Ispitivanje genetičke varijabilnosti i korelacije haplotipova medonosne pčele Apis mellifera i pčelinjeg krpelja Varroa destructor
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5873
ER  - 

@phdthesis{
author = "Gajić, Bojan",
year = "2016",
abstract = "In this study, genetic variability of honey bee Apis mellifera and honey bee mite Varroa destructor populations was analyzed, monitoring haplotype-based distribution pattern between the host and the parasite. For the identification of honey bee haplotypes, samples from 48 honey bee colonies were used, while 245 Varroa mite samples originating from 29 colonies were analyzed for haplotype identification and monitoring correlation between A. mellifera and V. destructor haplotypes. Sequencing of tRNAleu-cox2 region revealed six A. mellifera haplotypes, with C2aa haplotype observed for the first time. Different frequency of identifed bee haplotypes was detected in total sample, with C2d haplotype being the most frequent, and C2aa and C2i haplotypes with the lowest frequency. Distribution of A. mellifera haplotypes was found to be dependent on the locality, with three sites represented by only one haplotype, one locality represented by two haplotypes and four localities represented by three honey bee haplotypes. Sequencing results of V. destructor mitochondrial fragments revealed the point mutations at position 1932 and 10133 mtDNA within cox1 and cytb sequences, respectively. Based on these polymorphic sites, two novel Varroa haplotypes were defined and named as Serbia 1 (S1) and Peshter 1 (P1). Both detected mutations were the silent ones and did not affect the protein sequence. At the sequence chromatograms in some of the analyzed samples, double peaks were detected at haplotype-defining sites within cox1 and cytb, observing the phenomenon of nucleotide heteroplasmy for the first time in V. destructor. After the polymorphic sites had been observed using the sequencing method, ARMS and RFLP assays were performed for identification of Varroa haplotypes and detection of heteroplasmic individuals. Results of the cox1 sequence analysis showed that 48,2% of individuals belonged to the K haplotype, 31,8% belonged to the S1 haplotype and 20,0% Varroa samples was found to be heteroplasmic..., U ovom radu ispitivana je genetička varijabilnost medonosne pčele Apis mellifera i pčelinjeg krpelja Varroa destructor, uz praćenje korelacije u distribuciji haplotipova domaćina i parazita. Za identifikaciju haplotipova pčela korišćeni su uzorci iz 48 pčelinjih društava sa osam lokaliteta na teritoriji Srbije, dok je za tipizaciju varoa i praćenje korelacije haplotipova domaćina i parazita analizirano 245 uzoraka varoe iz 29 košnica. Sekvencioniranjem tRNAleu-cox2 regiona definisano je šest haplotipova A. mellifera, pri čemu je haplotip C2aa opisan po prvi put. Zastupljenost identifikovanih haplotipova pčela razlikovala se u ukupnom uzorku, pri čemu je najzastupljeniji bio haplotip C2d, dok su najmanje prisutni bili haplotipovi C2aa i C2i. Distribucija haplotipova A. mellifera razlikovala se u zavisnosti od lokaliteta, pri čemu je na tri posmatrana lokaliteta utvrđen po jedan haplotip, na jednom lokalitetu dva i na četiri lokaliteta utvrđeno je prisustvo tri haplotipa pčela. Rezultati sekvencioniranja delova mitohondrijalnih gena V. destructor otkrili su prisustvo tačkastih mutacija na pozicijama 1932 i 10133 mtDNK unutar cox1 i cytb sekvenci. Na osnovu tih polimorfnih mesta, definisana su dva nova haplotipa varoe koji su nazvani Srbija 1 (S1) i Pešter 1 (P1). Obe otkrivene mutacije po svojoj prirodi spadaju u „tihe‟ mutacije koje ne dovode do promene proteinskog lanca. Na hromatogramima pojedinih uzoraka otkriveni su dupli pikovi na haplotip definišućim mestima unutar cox1 i cytb, čime je po prvi put utvrđena nukleotidna heteroplazmija kod vrste V. destructor. Nakon utvrđivanja polimorfnih mesta metodom sekvencioniranja, za identifikaciju haplotipova varoe i otkrivanje jedinki sa heteroplazmijom korišćene su ARMS i RFLP metode. Analizom cox1 sekvence ARMS metodom utvrđeno je 48,2% jedinki K haplotipa, 31,8% jedinki S1 haplotipa i 20,0% jedinki sa heteroplazmijom...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Analysis of genetic variability and haplotype correlation between honey bee Apis mellifera and honey bee mite Varroa destructor, Ispitivanje genetičke varijabilnosti i korelacije haplotipova medonosne pčele Apis mellifera i pčelinjeg krpelja Varroa destructor",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5873"
}

Gajić, B.. (2016). Analysis of genetic variability and haplotype correlation between honey bee Apis mellifera and honey bee mite Varroa destructor. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_5873

Gajić B. Analysis of genetic variability and haplotype correlation between honey bee Apis mellifera and honey bee mite Varroa destructor. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_5873 .

Gajić, Bojan, "Analysis of genetic variability and haplotype correlation between honey bee Apis mellifera and honey bee mite Varroa destructor" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_5873 .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Stevanović, Jevrosima
AU  - Radulović, Željko
AU  - Kulišić, Zoran
AU  - Vejnović, Branislav
AU  - Glavinić, Uroš
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1422
AB  - In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods
VL  - 70
IS  - 3
SP  - 287
EP  - 297
DO  - 10.1007/s10493-016-0086-6
ER  - 

@article{
author = "Gajić, Bojan and Stevanović, Jevrosima and Radulović, Željko and Kulišić, Zoran and Vejnović, Branislav and Glavinić, Uroš and Stanimirović, Zoran",
year = "2016",
abstract = "In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods",
volume = "70",
number = "3",
pages = "287-297",
doi = "10.1007/s10493-016-0086-6"
}

Gajić, B., Stevanović, J., Radulović, Ž., Kulišić, Z., Vejnović, B., Glavinić, U.,& Stanimirović, Z.. (2016). Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology
Springer, Dordrecht., 70(3), 287-297.
https://doi.org/10.1007/s10493-016-0086-6

Gajić B, Stevanović J, Radulović Ž, Kulišić Z, Vejnović B, Glavinić U, Stanimirović Z. Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology. 2016;70(3):287-297.
doi:10.1007/s10493-016-0086-6 .

Gajić, Bojan, Stevanović, Jevrosima, Radulović, Željko, Kulišić, Zoran, Vejnović, Branislav, Glavinić, Uroš, Stanimirović, Zoran, "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods" in Experimental and Applied Acarology, 70, no. 3 (2016):287-297,
https://doi.org/10.1007/s10493-016-0086-6 . .

TY  - JOUR
AU  - Ozvegy, Jozsef
AU  - Marinković, Darko
AU  - Vučićević, Miloš
AU  - Gajić, Bojan
AU  - Stevanović, Jevrosima
AU  - Krnjaić, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1309
AB  - Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo
VL  - 65
IS  - 4
SP  - 443
EP  - 453
DO  - 10.1515/acve-2015-0037
ER  - 

@article{
author = "Ozvegy, Jozsef and Marinković, Darko and Vučićević, Miloš and Gajić, Bojan and Stevanović, Jevrosima and Krnjaić, Dejan and Aleksić-Kovačević, Sanja",
year = "2015",
abstract = "Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo",
volume = "65",
number = "4",
pages = "443-453",
doi = "10.1515/acve-2015-0037"
}

Ozvegy, J., Marinković, D., Vučićević, M., Gajić, B., Stevanović, J., Krnjaić, D.,& Aleksić-Kovačević, S.. (2015). Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 443-453.
https://doi.org/10.1515/acve-2015-0037

Ozvegy J, Marinković D, Vučićević M, Gajić B, Stevanović J, Krnjaić D, Aleksić-Kovačević S. Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo. in Acta Veterinaria-Beograd. 2015;65(4):443-453.
doi:10.1515/acve-2015-0037 .

Ozvegy, Jozsef, Marinković, Darko, Vučićević, Miloš, Gajić, Bojan, Stevanović, Jevrosima, Krnjaić, Dejan, Aleksić-Kovačević, Sanja, "Cytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zoo" in Acta Veterinaria-Beograd, 65, no. 4 (2015):443-453,
https://doi.org/10.1515/acve-2015-0037 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Stevanović, Jevrosima
AU  - Kulišić, Zoran
AU  - Simeunović, Predrag
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1152
AB  - Thelazia callipaeda is a parasitic nematode causing ocular infections in different mammalian species and humans, clinically manifested as lacrimation, conjunctivitis, keratitis, corneal opacity or corneal ulcer. In this paper, we reported six cases of autochthonous canine and feline thelaziosis on different localities in Serbia. Total of 285 parasites (85 males and 200 females) were collected from the eyes of infected animals (n=6) suffering from uni- or bilateral conjunctivitis, with the number of parasites ranging from 7 to 150 per animal. All parasites were morphologically identified as T. callipaeda, while molecular analyses of cytochrome oxidase 1 (cox1) gene revealed the presence of h1 haplotype, as the unique previously reported in other studies in Europe. Since T. callipaeda is a newly detected parasite in Serbia with the infective potential for humans, there is a necessity for animal owners education and cooperation among professional services in order to control this zoonosis.
AB  - Thelazia callipaeda je parazitska nematoda koja izaziva infekciju oka različitih vrsta sisara i ljudi, a koja se klinički manifestuje pojačanim suzenjem, pojavom konjuktivitisa, keratitisa, zamućenja rožnjače ili pojave ulcera na rožnjači. U ovom radu opisani su nalazi autohtone telazioze pasa i mačaka na šest različitih lokaliteta u Srbiji. Iz očiju inficiranih životinja (n=6) sa simptomima uni- ili bilateralnog konjuktivitisa sakupljeno je 285 parazita (85 mužjaka i 200 ženki), pri čemu se broj parazita po životinji kretao od 7 do 150. Svi paraziti morfološki su identifikovani kao T. callipaeda, dok su molekularne analize citohrom oksidaza 1 (cox1) gena utvrdile postojanje h1 haplotipa, koji je do sada i jedini utvrđeni haplotip ovog parazita u Evropi. S obzirom da se radi o novootkrivenom parazitu na teritoriji Srbije koji može inficirati i ljude, neophodno je uložiti napore u edukaciju vlasnika životinja i saradnju stručnih službi u cilju kontrole ove zoonoze.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia
T1  - Telazioza pasa i mačaka izazvana sa Thelazia callipaeda u Srbiji
VL  - 64
IS  - 4
SP  - 447
EP  - 455
DO  - 10.2478/acve-2014-0042
ER  - 

@article{
author = "Gajić, Bojan and Bogunović, Danica and Stevanović, Jevrosima and Kulišić, Zoran and Simeunović, Predrag and Stanimirović, Zoran",
year = "2014",
abstract = "Thelazia callipaeda is a parasitic nematode causing ocular infections in different mammalian species and humans, clinically manifested as lacrimation, conjunctivitis, keratitis, corneal opacity or corneal ulcer. In this paper, we reported six cases of autochthonous canine and feline thelaziosis on different localities in Serbia. Total of 285 parasites (85 males and 200 females) were collected from the eyes of infected animals (n=6) suffering from uni- or bilateral conjunctivitis, with the number of parasites ranging from 7 to 150 per animal. All parasites were morphologically identified as T. callipaeda, while molecular analyses of cytochrome oxidase 1 (cox1) gene revealed the presence of h1 haplotype, as the unique previously reported in other studies in Europe. Since T. callipaeda is a newly detected parasite in Serbia with the infective potential for humans, there is a necessity for animal owners education and cooperation among professional services in order to control this zoonosis., Thelazia callipaeda je parazitska nematoda koja izaziva infekciju oka različitih vrsta sisara i ljudi, a koja se klinički manifestuje pojačanim suzenjem, pojavom konjuktivitisa, keratitisa, zamućenja rožnjače ili pojave ulcera na rožnjači. U ovom radu opisani su nalazi autohtone telazioze pasa i mačaka na šest različitih lokaliteta u Srbiji. Iz očiju inficiranih životinja (n=6) sa simptomima uni- ili bilateralnog konjuktivitisa sakupljeno je 285 parazita (85 mužjaka i 200 ženki), pri čemu se broj parazita po životinji kretao od 7 do 150. Svi paraziti morfološki su identifikovani kao T. callipaeda, dok su molekularne analize citohrom oksidaza 1 (cox1) gena utvrdile postojanje h1 haplotipa, koji je do sada i jedini utvrđeni haplotip ovog parazita u Evropi. S obzirom da se radi o novootkrivenom parazitu na teritoriji Srbije koji može inficirati i ljude, neophodno je uložiti napore u edukaciju vlasnika životinja i saradnju stručnih službi u cilju kontrole ove zoonoze.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia, Telazioza pasa i mačaka izazvana sa Thelazia callipaeda u Srbiji",
volume = "64",
number = "4",
pages = "447-455",
doi = "10.2478/acve-2014-0042"
}

Gajić, B., Bogunović, D., Stevanović, J., Kulišić, Z., Simeunović, P.,& Stanimirović, Z.. (2014). Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(4), 447-455.
https://doi.org/10.2478/acve-2014-0042

Gajić B, Bogunović D, Stevanović J, Kulišić Z, Simeunović P, Stanimirović Z. Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia. in Acta Veterinaria-Beograd. 2014;64(4):447-455.
doi:10.2478/acve-2014-0042 .

Gajić, Bojan, Bogunović, Danica, Stevanović, Jevrosima, Kulišić, Zoran, Simeunović, Predrag, Stanimirović, Zoran, "Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia" in Acta Veterinaria-Beograd, 64, no. 4 (2014):447-455,
https://doi.org/10.2478/acve-2014-0042 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Gajić, Bojan
AU  - Simeunović, Predrag
AU  - Đurić, Spomenka
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1148
AB  - Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues).
AB  - Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor
T1  - DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor
VL  - 64
IS  - 3
SP  - 349
EP  - 357
DO  - 10.2478/acve-2014-0033
ER  - 

@article{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Gajić, Bojan and Simeunović, Predrag and Đurić, Spomenka and Vejnović, Branislav and Stanimirović, Zoran",
year = "2014",
abstract = "Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues)., Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor, DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor",
volume = "64",
number = "3",
pages = "349-357",
doi = "10.2478/acve-2014-0033"
}

Glavinić, U., Stevanović, J., Gajić, B., Simeunović, P., Đurić, S., Vejnović, B.,& Stanimirović, Z.. (2014). Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3), 349-357.
https://doi.org/10.2478/acve-2014-0033

Glavinić U, Stevanović J, Gajić B, Simeunović P, Đurić S, Vejnović B, Stanimirović Z. Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd. 2014;64(3):349-357.
doi:10.2478/acve-2014-0033 .

Glavinić, Uroš, Stevanović, Jevrosima, Gajić, Bojan, Simeunović, Predrag, Đurić, Spomenka, Vejnović, Branislav, Stanimirović, Zoran, "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor" in Acta Veterinaria-Beograd, 64, no. 3 (2014):349-357,
https://doi.org/10.2478/acve-2014-0033 . .

TY  - JOUR
AU  - Kulišić, Zoran
AU  - Aleksić, Nevenka
AU  - Đorđević, Milutin
AU  - Gajić, Bojan
AU  - Tambur, Zoran
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1049
AB  - A coprological examination of 680 grazing sheep was performed in Eastern Serbia from March 2011 to November 2012 in order to determine the presence of gastrointestinal (GI) nematode parasites. Fecal samples were randomly collected and examined by using qualitative and quantitative coprological techniques. It was found that 74.56% sheep were infected. Samples that contained nematode eggs were processed for larval development and eleven nematode genera were identified: Haemonchus (46.91%), Ostertagia (25.88%), Marshallagia (21.91%), Cooperia (14.12%), Trichostrongylus (39.85%), Nematodirus (35.88%), Bunostomum (23.97%), Strongyloides (17.06%) Oesophagostomum (40.73%), Chabertia (32.79%) and Trichuris (10.88%). Higher prevalence of infection was observed in females (p lt 0.01), as well as in adults (p lt 0.001). Regarding the intensity of infection, in 40.63% sheep it was low, in 51.87% moderate and in 7.50% high. There was no difference in intensity of infection considering sex and age of animals. Moreover, simultaneous infection with different number of nematode genera was dependent on sheep's age (p lt 0.001). These results suggest that GI nematodes are a conspicuous problem of grazing sheep in the study area.
AB  - U periodu od marta 2011. do novembra 2012. godine, na teritoriji Istočne Srbije sprovedeno je koprološko ispitivanje 680 ovaca držanih u pašnom načinu gajenja na prisustvo gastrointestinalnih (GI) nematoda. Uzorci fecesa uzimani su metodom slučajnog uzorka i ispitivani korišćenjem kvalitativnih i kvantitativnih koproloških tehnika. Ispitivanjem je utvrđeno 74,56% inficiranih ovaca. Uzorci u kojima su pronađena jaja nematoda podvrgnuti su determinaciji larvi, pri čemu je identifikovano jedanaest rodova nematoda: Haemonchus (46,91%), Ostertagia (25,88%), Marshallagia (21,91%), Cooperia (14,12%), Trichostrongylus (39,85%), Nematodirus (35,88%), Bunostomum (23,97%), Strongyloides (17,06%) Oesophagostomum (40,73%), Chabertia (32,79%) i Trichuris (10,88%). Veća prevalencija infekcije uočena je kod ženki (p lt 0,01), kao i kod adultnih životinja (p lt 0,001). Kod 40,63% ovaca, infekcija je bila niskog, kod 51,87% umerenog, a kod 7,50% visokog intenziteta. Nisu dokazane razlike u intenzitetu infekcije u zavisnosti od pola i starosti životinja. Istovremena infekcija sa više rodova nematoda zavisila je od starosti ovaca (p lt 0,001). Dobijeni rezultati upućuju na zaključak da su GI nematode značajan problem ovaca držanih na pašnjaku u ispitivanom području.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence and intensity of infection with gastrointestinal nematodes in sheep in eastern Serbia
T1  - Prevalencija i intenzitet infekcije gastrointestinalnim nematodama kod ovaca u istočnoj Srbiji
VL  - 63
IS  - 4
SP  - 429
EP  - 436
DO  - 10.2298/AVB1304429K
ER  - 

@article{
author = "Kulišić, Zoran and Aleksić, Nevenka and Đorđević, Milutin and Gajić, Bojan and Tambur, Zoran and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2013",
abstract = "A coprological examination of 680 grazing sheep was performed in Eastern Serbia from March 2011 to November 2012 in order to determine the presence of gastrointestinal (GI) nematode parasites. Fecal samples were randomly collected and examined by using qualitative and quantitative coprological techniques. It was found that 74.56% sheep were infected. Samples that contained nematode eggs were processed for larval development and eleven nematode genera were identified: Haemonchus (46.91%), Ostertagia (25.88%), Marshallagia (21.91%), Cooperia (14.12%), Trichostrongylus (39.85%), Nematodirus (35.88%), Bunostomum (23.97%), Strongyloides (17.06%) Oesophagostomum (40.73%), Chabertia (32.79%) and Trichuris (10.88%). Higher prevalence of infection was observed in females (p lt 0.01), as well as in adults (p lt 0.001). Regarding the intensity of infection, in 40.63% sheep it was low, in 51.87% moderate and in 7.50% high. There was no difference in intensity of infection considering sex and age of animals. Moreover, simultaneous infection with different number of nematode genera was dependent on sheep's age (p lt 0.001). These results suggest that GI nematodes are a conspicuous problem of grazing sheep in the study area., U periodu od marta 2011. do novembra 2012. godine, na teritoriji Istočne Srbije sprovedeno je koprološko ispitivanje 680 ovaca držanih u pašnom načinu gajenja na prisustvo gastrointestinalnih (GI) nematoda. Uzorci fecesa uzimani su metodom slučajnog uzorka i ispitivani korišćenjem kvalitativnih i kvantitativnih koproloških tehnika. Ispitivanjem je utvrđeno 74,56% inficiranih ovaca. Uzorci u kojima su pronađena jaja nematoda podvrgnuti su determinaciji larvi, pri čemu je identifikovano jedanaest rodova nematoda: Haemonchus (46,91%), Ostertagia (25,88%), Marshallagia (21,91%), Cooperia (14,12%), Trichostrongylus (39,85%), Nematodirus (35,88%), Bunostomum (23,97%), Strongyloides (17,06%) Oesophagostomum (40,73%), Chabertia (32,79%) i Trichuris (10,88%). Veća prevalencija infekcije uočena je kod ženki (p lt 0,01), kao i kod adultnih životinja (p lt 0,001). Kod 40,63% ovaca, infekcija je bila niskog, kod 51,87% umerenog, a kod 7,50% visokog intenziteta. Nisu dokazane razlike u intenzitetu infekcije u zavisnosti od pola i starosti životinja. Istovremena infekcija sa više rodova nematoda zavisila je od starosti ovaca (p lt 0,001). Dobijeni rezultati upućuju na zaključak da su GI nematode značajan problem ovaca držanih na pašnjaku u ispitivanom području.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence and intensity of infection with gastrointestinal nematodes in sheep in eastern Serbia, Prevalencija i intenzitet infekcije gastrointestinalnim nematodama kod ovaca u istočnoj Srbiji",
volume = "63",
number = "4",
pages = "429-436",
doi = "10.2298/AVB1304429K"
}

Kulišić, Z., Aleksić, N., Đorđević, M., Gajić, B., Tambur, Z., Stevanović, J.,& Stanimirović, Z.. (2013). Prevalence and intensity of infection with gastrointestinal nematodes in sheep in eastern Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 429-436.
https://doi.org/10.2298/AVB1304429K

Kulišić Z, Aleksić N, Đorđević M, Gajić B, Tambur Z, Stevanović J, Stanimirović Z. Prevalence and intensity of infection with gastrointestinal nematodes in sheep in eastern Serbia. in Acta Veterinaria-Beograd. 2013;63(4):429-436.
doi:10.2298/AVB1304429K .

Kulišić, Zoran, Aleksić, Nevenka, Đorđević, Milutin, Gajić, Bojan, Tambur, Zoran, Stevanović, Jevrosima, Stanimirović, Zoran, "Prevalence and intensity of infection with gastrointestinal nematodes in sheep in eastern Serbia" in Acta Veterinaria-Beograd, 63, no. 4 (2013):429-436,
https://doi.org/10.2298/AVB1304429K . .

TY  - JOUR
AU  - Vučićević, Miloš
AU  - Stevanov-Pavlović, Marija
AU  - Stevanović, Jevrosima
AU  - Bošnjak, Jasna
AU  - Gajić, Bojan
AU  - Aleksić, Nevenka
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1021
AB  - The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269-276, 2013.
PB  - Wiley-Blackwell, Hoboken
T2  - Zoo Biology
T1  - Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing
VL  - 32
IS  - 3
SP  - 269
EP  - 276
DO  - 10.1002/zoo.21010
ER  - 

@article{
author = "Vučićević, Miloš and Stevanov-Pavlović, Marija and Stevanović, Jevrosima and Bošnjak, Jasna and Gajić, Bojan and Aleksić, Nevenka and Stanimirović, Zoran",
year = "2013",
abstract = "The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269-276, 2013.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Zoo Biology",
title = "Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing",
volume = "32",
number = "3",
pages = "269-276",
doi = "10.1002/zoo.21010"
}

Vučićević, M., Stevanov-Pavlović, M., Stevanović, J., Bošnjak, J., Gajić, B., Aleksić, N.,& Stanimirović, Z.. (2013). Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. in Zoo Biology
Wiley-Blackwell, Hoboken., 32(3), 269-276.
https://doi.org/10.1002/zoo.21010

Vučićević M, Stevanov-Pavlović M, Stevanović J, Bošnjak J, Gajić B, Aleksić N, Stanimirović Z. Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing. in Zoo Biology. 2013;32(3):269-276.
doi:10.1002/zoo.21010 .

Vučićević, Miloš, Stevanov-Pavlović, Marija, Stevanović, Jevrosima, Bošnjak, Jasna, Gajić, Bojan, Aleksić, Nevenka, Stanimirović, Zoran, "Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing" in Zoo Biology, 32, no. 3 (2013):269-276,
https://doi.org/10.1002/zoo.21010 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Radulović, Željko
AU  - Stevanović, Jevrosima
AU  - Kulišić, Zoran
AU  - Vučićević, Miloš
AU  - Simeunović, Predrag
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1050
AB  - Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis
VL  - 61
IS  - 1
SP  - 97
EP  - 105
DO  - 10.1007/s10493-013-9683-9
ER  - 

@article{
author = "Gajić, Bojan and Radulović, Željko and Stevanović, Jevrosima and Kulišić, Zoran and Vučićević, Miloš and Simeunović, Predrag and Stanimirović, Zoran",
year = "2013",
abstract = "Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis",
volume = "61",
number = "1",
pages = "97-105",
doi = "10.1007/s10493-013-9683-9"
}

Gajić, B., Radulović, Ž., Stevanović, J., Kulišić, Z., Vučićević, M., Simeunović, P.,& Stanimirović, Z.. (2013). Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology
Springer, Dordrecht., 61(1), 97-105.
https://doi.org/10.1007/s10493-013-9683-9

Gajić B, Radulović Ž, Stevanović J, Kulišić Z, Vučićević M, Simeunović P, Stanimirović Z. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology. 2013;61(1):97-105.
doi:10.1007/s10493-013-9683-9 .

Gajić, Bojan, Radulović, Željko, Stevanović, Jevrosima, Kulišić, Zoran, Vučićević, Miloš, Simeunović, Predrag, Stanimirović, Zoran, "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis" in Experimental and Applied Acarology, 61, no. 1 (2013):97-105,
https://doi.org/10.1007/s10493-013-9683-9 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Simeunović, Predrag
AU  - Gajić, Bojan
AU  - Lakić, Nada
AU  - Radović, Dejan
AU  - Fries, Ingemar
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1076
AB  - The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.
PB  - Springer France, Paris
T2  - Apidologie
T1  - Characteristics of Nosema ceranae infection in Serbian honey bee colonies
VL  - 44
IS  - 5
SP  - 522
EP  - 536
DO  - 10.1007/s13592-013-0203-z
ER  - 

@article{
author = "Stevanović, Jevrosima and Simeunović, Predrag and Gajić, Bojan and Lakić, Nada and Radović, Dejan and Fries, Ingemar and Stanimirović, Zoran",
year = "2013",
abstract = "The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.",
publisher = "Springer France, Paris",
journal = "Apidologie",
title = "Characteristics of Nosema ceranae infection in Serbian honey bee colonies",
volume = "44",
number = "5",
pages = "522-536",
doi = "10.1007/s13592-013-0203-z"
}

Stevanović, J., Simeunović, P., Gajić, B., Lakić, N., Radović, D., Fries, I.,& Stanimirović, Z.. (2013). Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie
Springer France, Paris., 44(5), 522-536.
https://doi.org/10.1007/s13592-013-0203-z

Stevanović J, Simeunović P, Gajić B, Lakić N, Radović D, Fries I, Stanimirović Z. Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie. 2013;44(5):522-536.
doi:10.1007/s13592-013-0203-z .

Stevanović, Jevrosima, Simeunović, Predrag, Gajić, Bojan, Lakić, Nada, Radović, Dejan, Fries, Ingemar, Stanimirović, Zoran, "Characteristics of Nosema ceranae infection in Serbian honey bee colonies" in Apidologie, 44, no. 5 (2013):522-536,
https://doi.org/10.1007/s13592-013-0203-z . .

TY  - JOUR
AU  - Kulišić, Zoran
AU  - Aleksić, Nevenka
AU  - Đorđević, Milutin
AU  - Gajić, Bojan
AU  - Tambur, Zoran
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/920
AB  - This study was undertaken to estimate the prevalence of gastrointestinal helminths in calves in western Serbia. Throughout 2011 faecal samples were collected from 600 calves aged up to 180 days, samples were examined with the flotation method and a modified McMaster technique. The parasitizing helminth species were identified and the level of infection compared between different age groups. As many as 64.17% animals were found to be infected. The following parasite species were diagnosed: Moniezia spp. (3.17%), Toxocara vitulorum (35.00%), Strongyloides papillosus (34.50%), gastrointestinal strongyles (4.50%) and Trichuris discolor (2.17%). The majority of calves were infected with two, fewer with three or one helminth species, and the smollest number of calves harboured four parasite species. The prevalence of established helminth infections varied depending on the calves' age.
AB  - Studija je sprovedena sa ciljem da se utvrdi prevalencija gastrointestinalnih helminata kod teladi u zapadnom delu Srbije. Tokom 2011. godine prikupljeni su uzorci fecesa ukupno 600 teladi starosti do 180 dana. Uzorci su pregledani metodom flotacije i modifikovanom metodom po McMasteru. Determinisane su vrste helminata i određena je prevalencija infekcije kod teladi različite starosti. Rezultati ispitivanja pokazali su da je infekcija helmintima bila prisutna kod 64,17% pregledanih životinja. Ustanovljene su sledeće vrste helminata: Moniezia spp. (3,17%), Toxocara vitulorum (35,00%), Strongyloides papillosus (34,50%), želudačnocrevne strongilide (4,50%) i Trichuris discolor (2,17%). Većina teladi istovremeno je bila inficirana dvema vrstama, zatim sa tri i jednom vrstom helminta, dok je kod samo nekoliko životinja bilo ustanovljeno prisustvo četiri vrste ili grupe helminata. Prevalencija infekcija helmintima razlikovala se kod teladi različite starosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence of gastrointestinal helminths in calves in Western Serbia
T1  - Prevalencija gastrointestinalnih helminata kod teladi u zapadnoj Srbiji
VL  - 62
IS  - 5-6
SP  - 665
EP  - 673
DO  - 10.2298/AVB1206665K
ER  - 

@article{
author = "Kulišić, Zoran and Aleksić, Nevenka and Đorđević, Milutin and Gajić, Bojan and Tambur, Zoran and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2012",
abstract = "This study was undertaken to estimate the prevalence of gastrointestinal helminths in calves in western Serbia. Throughout 2011 faecal samples were collected from 600 calves aged up to 180 days, samples were examined with the flotation method and a modified McMaster technique. The parasitizing helminth species were identified and the level of infection compared between different age groups. As many as 64.17% animals were found to be infected. The following parasite species were diagnosed: Moniezia spp. (3.17%), Toxocara vitulorum (35.00%), Strongyloides papillosus (34.50%), gastrointestinal strongyles (4.50%) and Trichuris discolor (2.17%). The majority of calves were infected with two, fewer with three or one helminth species, and the smollest number of calves harboured four parasite species. The prevalence of established helminth infections varied depending on the calves' age., Studija je sprovedena sa ciljem da se utvrdi prevalencija gastrointestinalnih helminata kod teladi u zapadnom delu Srbije. Tokom 2011. godine prikupljeni su uzorci fecesa ukupno 600 teladi starosti do 180 dana. Uzorci su pregledani metodom flotacije i modifikovanom metodom po McMasteru. Determinisane su vrste helminata i određena je prevalencija infekcije kod teladi različite starosti. Rezultati ispitivanja pokazali su da je infekcija helmintima bila prisutna kod 64,17% pregledanih životinja. Ustanovljene su sledeće vrste helminata: Moniezia spp. (3,17%), Toxocara vitulorum (35,00%), Strongyloides papillosus (34,50%), želudačnocrevne strongilide (4,50%) i Trichuris discolor (2,17%). Većina teladi istovremeno je bila inficirana dvema vrstama, zatim sa tri i jednom vrstom helminta, dok je kod samo nekoliko životinja bilo ustanovljeno prisustvo četiri vrste ili grupe helminata. Prevalencija infekcija helmintima razlikovala se kod teladi različite starosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence of gastrointestinal helminths in calves in Western Serbia, Prevalencija gastrointestinalnih helminata kod teladi u zapadnoj Srbiji",
volume = "62",
number = "5-6",
pages = "665-673",
doi = "10.2298/AVB1206665K"
}

Kulišić, Z., Aleksić, N., Đorđević, M., Gajić, B., Tambur, Z., Stevanović, J.,& Stanimirović, Z.. (2012). Prevalence of gastrointestinal helminths in calves in Western Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 665-673.
https://doi.org/10.2298/AVB1206665K

Kulišić Z, Aleksić N, Đorđević M, Gajić B, Tambur Z, Stevanović J, Stanimirović Z. Prevalence of gastrointestinal helminths in calves in Western Serbia. in Acta Veterinaria-Beograd. 2012;62(5-6):665-673.
doi:10.2298/AVB1206665K .

Kulišić, Zoran, Aleksić, Nevenka, Đorđević, Milutin, Gajić, Bojan, Tambur, Zoran, Stevanović, Jevrosima, Stanimirović, Zoran, "Prevalence of gastrointestinal helminths in calves in Western Serbia" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):665-673,
https://doi.org/10.2298/AVB1206665K . .