TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2020
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier
T2  - Mutation Research/Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
SP  - 108371
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier",
journal = "Mutation Research/Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
pages = "108371",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research
Elsevier., 787, 108371.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research. 2021;787:108371.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research/Reviews in Mutation Research, 787 (2021):108371,
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Ivanović, Saša R.
AU  - Borozan, Nevena
AU  - Janković, Radmila
AU  - Ćupić Miladinović, Dejana
AU  - Savić, Mila
AU  - Ćupić, Vitomir
AU  - Borozan, Sunčica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2249
AB  - Background/Aim. Organophosphate pesticides (OPs) are used extensively worldwide in agriculture and forestry, and their application represents a major health problem for humans and animals. The aim of this study was to investigate the possibility of the adaptation of an organism to the prolonged administration of a low dose of diazinon. Methods. The study was conducted on a total of 60 male Wistar rats. The first 30 rats were divided into four equal diazinon groups (n = 6) and the control one (corn oil). Diazinon was orally administered once at doses: 200, 400, 600, 800 mg/kg (one dose – one group). The concentration of glucose, the activity of α-amylase and the relative activity of LDH1-LDH5 isoenzymes in the blood were measured 24 hours after the application. The remaining 30 rats were divided into two equal diazinon groups (n = 10) and the control one (corn oil). The first group was treated during 7 days, and the second during 14 days with 55 mg/kg of diazinon (1/10 of previously determined LD50 value). The histopathology of the pancreas and the liver, as well as the relative activities of LDH isoenzymes in the blood, were determined after the completion of both time periods. Results. Single administration of increasing doses of diazinon resulted in a significant increase in the concentrations of glucose, activity of α-amylase and LDH isoenzymes. Subacute application of a low diazinon dose induced histopathological changes in the pancreas manifested by acinar cell necrosis, and in the liver in the form of portal hepatitis and multifocal necrosis. The cumulative doses resulted in statistically significantly lower activities of LDH isoenzymes compared with the single administration of these doses, indicating a lower degree of the cells damage after the subacute diazinon administration. Conclusion. Subacute administration of a low dose of diazinon leads to a different adaptation degree of organs and organ systems to toxic effects caused by this organophosphate.
AB  - Uvod/Cilj. Organofosfatni pesticidi (OP) se intenzivno koriste širom sveta u poljoprivredi i šumarstvu, a njihova primena predstavlja značajan zdravstveni problem kod ljudi i životinja. Cilj ove studije bio je da se ispita mogućnost adaptacije organizma na prolongiranu primenu niskih doza diazinona. Metode. Studija je sprovedena na ukupno 60 pacova muškog pola Vistar soja. Prvih 30 pacova je podel-jeno u četiri jednake grupe tretirane diazinonom (n = 6) i kontrolnu grupu (kukuruzno ulje). Diazinon je primenjivan jednokratno peroralno u dozama: 200, 400, 600, 800 mg/kg (jedna doza – jedna grupa). Koncentracija glukoze, aktivnost α-amilaze i relativna aktivnost LDH1-LDH5 izoenzima u krvi, određivani su 24 sata nakon aplikacije. Preostalih 30 pacova je podeljeno u dve jednake diazinon grupe (n = 10) i kontrolnu grupu (kukuruzno ulje). Prva grupa je tretirana 7 dana, a druga 14 dana sa 55 mg/kg diazinona (1/10 prethod-no određene vrednosti LD50). Histopatologija pankreasa i jetre i određivanje relativne aktivnosti LDH izoenzima u krvi urađeni su po završetku oba vremenska perioda. Rezultati. Jednokratna primena rastućih doza diazinona rezultirala je statistički značajnim povećanjem koncentracije glukoze, ak-tivnosti α-amilaze i LDH izoenzima. Subakutna primena niske doze diazinona indukovala je histopatološke promene u pankreasu manifestovane acinarnom nekrozom, a u jetri promene su se ispoljile u vidu portalnog hepatitisa i multi-fokalne nekroze. Кumulativne doze diazinona rezultirale su statistički značajno nižom aktivnošću LDH izoenzima u poređenju sa jednokratnom primenom tih doza, što ukazuje na niži stepen oštećenja ćelija posle subakutne primene dia-zinona. Zaključak. Subakutna primena niske doze diazinona dovodi do različitog stepena adaptacije organa i organskih sistema na toksične efekte izazvane tim organofosfatom.
PB  - Savezno Ministarstvo Odbrane, Beograd
T2  - Vojnosanitetski pregled
T1  - Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon
T1  - Funkcionalne i histološke promene pankreasa i jetre kod pacova posle akutne i subakutne primene diazinona
VL  - 78
IS  - 9
SP  - 955
EP  - 963
DO  - 10.2298/VSP191223012I
ER  - 

@article{
author = "Ivanović, Saša R. and Borozan, Nevena and Janković, Radmila and Ćupić Miladinović, Dejana and Savić, Mila and Ćupić, Vitomir and Borozan, Sunčica",
year = "2021",
abstract = "Background/Aim. Organophosphate pesticides (OPs) are used extensively worldwide in agriculture and forestry, and their application represents a major health problem for humans and animals. The aim of this study was to investigate the possibility of the adaptation of an organism to the prolonged administration of a low dose of diazinon. Methods. The study was conducted on a total of 60 male Wistar rats. The first 30 rats were divided into four equal diazinon groups (n = 6) and the control one (corn oil). Diazinon was orally administered once at doses: 200, 400, 600, 800 mg/kg (one dose – one group). The concentration of glucose, the activity of α-amylase and the relative activity of LDH1-LDH5 isoenzymes in the blood were measured 24 hours after the application. The remaining 30 rats were divided into two equal diazinon groups (n = 10) and the control one (corn oil). The first group was treated during 7 days, and the second during 14 days with 55 mg/kg of diazinon (1/10 of previously determined LD50 value). The histopathology of the pancreas and the liver, as well as the relative activities of LDH isoenzymes in the blood, were determined after the completion of both time periods. Results. Single administration of increasing doses of diazinon resulted in a significant increase in the concentrations of glucose, activity of α-amylase and LDH isoenzymes. Subacute application of a low diazinon dose induced histopathological changes in the pancreas manifested by acinar cell necrosis, and in the liver in the form of portal hepatitis and multifocal necrosis. The cumulative doses resulted in statistically significantly lower activities of LDH isoenzymes compared with the single administration of these doses, indicating a lower degree of the cells damage after the subacute diazinon administration. Conclusion. Subacute administration of a low dose of diazinon leads to a different adaptation degree of organs and organ systems to toxic effects caused by this organophosphate., Uvod/Cilj. Organofosfatni pesticidi (OP) se intenzivno koriste širom sveta u poljoprivredi i šumarstvu, a njihova primena predstavlja značajan zdravstveni problem kod ljudi i životinja. Cilj ove studije bio je da se ispita mogućnost adaptacije organizma na prolongiranu primenu niskih doza diazinona. Metode. Studija je sprovedena na ukupno 60 pacova muškog pola Vistar soja. Prvih 30 pacova je podel-jeno u četiri jednake grupe tretirane diazinonom (n = 6) i kontrolnu grupu (kukuruzno ulje). Diazinon je primenjivan jednokratno peroralno u dozama: 200, 400, 600, 800 mg/kg (jedna doza – jedna grupa). Koncentracija glukoze, aktivnost α-amilaze i relativna aktivnost LDH1-LDH5 izoenzima u krvi, određivani su 24 sata nakon aplikacije. Preostalih 30 pacova je podeljeno u dve jednake diazinon grupe (n = 10) i kontrolnu grupu (kukuruzno ulje). Prva grupa je tretirana 7 dana, a druga 14 dana sa 55 mg/kg diazinona (1/10 prethod-no određene vrednosti LD50). Histopatologija pankreasa i jetre i određivanje relativne aktivnosti LDH izoenzima u krvi urađeni su po završetku oba vremenska perioda. Rezultati. Jednokratna primena rastućih doza diazinona rezultirala je statistički značajnim povećanjem koncentracije glukoze, ak-tivnosti α-amilaze i LDH izoenzima. Subakutna primena niske doze diazinona indukovala je histopatološke promene u pankreasu manifestovane acinarnom nekrozom, a u jetri promene su se ispoljile u vidu portalnog hepatitisa i multi-fokalne nekroze. Кumulativne doze diazinona rezultirale su statistički značajno nižom aktivnošću LDH izoenzima u poređenju sa jednokratnom primenom tih doza, što ukazuje na niži stepen oštećenja ćelija posle subakutne primene dia-zinona. Zaključak. Subakutna primena niske doze diazinona dovodi do različitog stepena adaptacije organa i organskih sistema na toksične efekte izazvane tim organofosfatom.",
publisher = "Savezno Ministarstvo Odbrane, Beograd",
journal = "Vojnosanitetski pregled",
title = "Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon, Funkcionalne i histološke promene pankreasa i jetre kod pacova posle akutne i subakutne primene diazinona",
volume = "78",
number = "9",
pages = "955-963",
doi = "10.2298/VSP191223012I"
}

Ivanović, S. R., Borozan, N., Janković, R., Ćupić Miladinović, D., Savić, M., Ćupić, V.,& Borozan, S.. (2021). Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon. in Vojnosanitetski pregled
Savezno Ministarstvo Odbrane, Beograd., 78(9), 955-963.
https://doi.org/10.2298/VSP191223012I

Ivanović SR, Borozan N, Janković R, Ćupić Miladinović D, Savić M, Ćupić V, Borozan S. Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon. in Vojnosanitetski pregled. 2021;78(9):955-963.
doi:10.2298/VSP191223012I .

Ivanović, Saša R., Borozan, Nevena, Janković, Radmila, Ćupić Miladinović, Dejana, Savić, Mila, Ćupić, Vitomir, Borozan, Sunčica, "Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon" in Vojnosanitetski pregled, 78, no. 9 (2021):955-963,
https://doi.org/10.2298/VSP191223012I . .

TY  - JOUR
AU  - Sjakste, Nikolajs
AU  - Đelić, Ninoslav
AU  - Dzintare, Maija
AU  - Živković, Lada
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1817
AB  - The review summarizes literature data on the DNA-binding, DNA-protecting and DNA-damaging activities of a range of natural human endogenous and exogenous compounds. Small natural organic molecules bind DNA in a site-specific mode, by arranging tight touch with the structure of the major and minor grooves, as well as individual bases in the local duplex DNA. Polyphenols are the best-studied exogenous compounds from this point of view. Many of them demonstrate hormetic effects, producing both beneficial and damaging effects. An attempt to establish the dependence of DNA damage or DNA protection on the concentration of the compound turned out to be successful for some polyphenols, daidzein, genistein and resveratrol, which were DNA protecting in low concentrations and DNA damaging in high concentrations. There was no evident dependence on concentration for quercetin and kaempferol. Probably, the DNA-protecting effect is associated with the affinity to DNA. Caffeine and theophylline are DNA binders; at the same time, they favor DNA repair. Although most alkaloids damage DNA, berberine can protect DNA against damage. Among the endogenous compounds, hormones belonging to the amine class, thyroid and steroid hormones appear to bind DNA and produce some DNA damage. Thus, natural compounds continue to reveal beneficial or adverse effects on genome integrity and provide a promising source of therapeutic activities.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts
VL  - 323
SP  - 109030
DO  - 10.1016/j.cbi.2020.109030
ER  - 

@article{
author = "Sjakste, Nikolajs and Đelić, Ninoslav and Dzintare, Maija and Živković, Lada",
year = "2020",
abstract = "The review summarizes literature data on the DNA-binding, DNA-protecting and DNA-damaging activities of a range of natural human endogenous and exogenous compounds. Small natural organic molecules bind DNA in a site-specific mode, by arranging tight touch with the structure of the major and minor grooves, as well as individual bases in the local duplex DNA. Polyphenols are the best-studied exogenous compounds from this point of view. Many of them demonstrate hormetic effects, producing both beneficial and damaging effects. An attempt to establish the dependence of DNA damage or DNA protection on the concentration of the compound turned out to be successful for some polyphenols, daidzein, genistein and resveratrol, which were DNA protecting in low concentrations and DNA damaging in high concentrations. There was no evident dependence on concentration for quercetin and kaempferol. Probably, the DNA-protecting effect is associated with the affinity to DNA. Caffeine and theophylline are DNA binders; at the same time, they favor DNA repair. Although most alkaloids damage DNA, berberine can protect DNA against damage. Among the endogenous compounds, hormones belonging to the amine class, thyroid and steroid hormones appear to bind DNA and produce some DNA damage. Thus, natural compounds continue to reveal beneficial or adverse effects on genome integrity and provide a promising source of therapeutic activities.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts",
volume = "323",
pages = "109030",
doi = "10.1016/j.cbi.2020.109030"
}

Sjakste, N., Đelić, N., Dzintare, M.,& Živković, L.. (2020). DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 323, 109030.
https://doi.org/10.1016/j.cbi.2020.109030

Sjakste N, Đelić N, Dzintare M, Živković L. DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts. in Chemico-Biological Interactions. 2020;323:109030.
doi:10.1016/j.cbi.2020.109030 .

Sjakste, Nikolajs, Đelić, Ninoslav, Dzintare, Maija, Živković, Lada, "DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts" in Chemico-Biological Interactions, 323 (2020):109030,
https://doi.org/10.1016/j.cbi.2020.109030 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, M.
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1849
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, M. and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract. in Genetika, 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, M., Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1791
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17 beta-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 mu M (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells antioxidant capacity.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
SP  - UNSP 402993
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17 beta-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 mu M (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells antioxidant capacity.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
pages = "UNSP 402993",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 845, UNSP 402993.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Spremo-Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 402993.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 402993,
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan Francisco
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1790
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cells repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - UNSP 403078
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan Francisco and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cells repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "UNSP 403078",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J. F.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 845, UNSP 403078.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez JF, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 403078.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan Francisco, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 403078,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1710
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Evaluation of antioxidant potential of Cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Bajić, Vladan and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Evaluation of antioxidant potential of Cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}

Živković, L., Borozan, S., Bajić, V., Đorđević, S., Hristov, A.,& Spremo-Potparević, B.. (2019). Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450

Živković L, Borozan S, Bajić V, Đorđević S, Hristov A, Spremo-Potparević B. Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis. 2019;53(4):129-134.
doi:10.5937/mckg53-24450 .

Živković, Lada, Borozan, Sunčica, Bajić, Vladan, Đorđević, Stefana, Hristov, Aleksandar, Spremo-Potparević, Biljana, "Evaluation of antioxidant potential of Cordyceps sinensis in vitro" in Medicinski časopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Saša
AU  - Ćupić Miladinović, Dejana
AU  - Ristanić, Marko
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1576
AB  - This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
VL  - 2018
SP  - 1805354
DO  - 10.1155/2018/1805354
ER  - 

@article{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Saša and Ćupić Miladinović, Dejana and Ristanić, Marko and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2018",
abstract = "This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline",
volume = "2018",
pages = "1805354",
doi = "10.1155/2018/1805354"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Ćupić Miladinović, D., Ristanić, M., Spremo-Potparević, B.,& Stanimirović, Z.. (2018). Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2018, 1805354.
https://doi.org/10.1155/2018/1805354

Radaković M, Borozan S, Đelić N, Ivanović S, Ćupić Miladinović D, Ristanić M, Spremo-Potparević B, Stanimirović Z. Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity. 2018;2018:1805354.
doi:10.1155/2018/1805354 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Saša, Ćupić Miladinović, Dejana, Ristanić, Marko, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline" in Oxidative Medicine and Cellular Longevity, 2018 (2018):1805354,
https://doi.org/10.1155/2018/1805354 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1619
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Spremo-Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Ćupić Miladinović, Dejana
AU  - Borozan, Sunčica
AU  - Ivanović, Saša
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1667
AB  - Chlorpyrifos is a widely used organophosphate pesticide (OP). In birds and mammals OP exhibits a toxic effect via inhibition of cholinesterases [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and through oxidative/nitrosative stress. In this study, the influence of chlorpyrifos on cholinesterase activity, parameters of oxidative stress [malondialdehyde (MDA); glutathione (GSH); superoxide dismutase (SOD); nitrite concentration (NO2-); hydrogen peroxide (H2O2)], and inflammatory parameter [activity of myeloperoxidase (MPO)] in the brain of Japanese quail (Coturnix japanica) was examined. The study was conducted on a total of 60 male Japanese quails (one control and 5 experimental groups, n = 10), 3 to 4 wk old. Quails were administered by gavage chlorpyrifos (CPF) for 7 consecutive da at doses of 0.375 mg/kg BW, 0.75 mg/kg BW, 1.5 mg/kg BW, 3 mg/kg BW, and 6 mg/kg BW. Our studies have shown that all doses of CPF significantly inhibited both cholinesterases in brain: AChE from 22.74 to 37.83% and BChE from 19.53 to 61.9%, and that inhibition was dose dependent. Also, CPF has led to an increase in the concentration of MDA, GSH, NO2-, and H2O2 and activity of SOD and MPO. Overall, these results support the hypothesis that CPF causes oxidative stress and inflammatory response. This research was carried out on quails because there is hardly any or not enough data about the neurotoxic effect of CPF and especially about its influence on oxidative stress in birds. This study is highly important because we are witnessing massive avian mortality in certain countries due to pesticides.
PB  - Oxford Univ Press, Oxford
T2  - Poultry Science
T1  - Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail
VL  - 97
IS  - 5
SP  - 1564
EP  - 1571
DO  - 10.3382/ps/pey018
ER  - 

@article{
author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Ivanović, Saša",
year = "2018",
abstract = "Chlorpyrifos is a widely used organophosphate pesticide (OP). In birds and mammals OP exhibits a toxic effect via inhibition of cholinesterases [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and through oxidative/nitrosative stress. In this study, the influence of chlorpyrifos on cholinesterase activity, parameters of oxidative stress [malondialdehyde (MDA); glutathione (GSH); superoxide dismutase (SOD); nitrite concentration (NO2-); hydrogen peroxide (H2O2)], and inflammatory parameter [activity of myeloperoxidase (MPO)] in the brain of Japanese quail (Coturnix japanica) was examined. The study was conducted on a total of 60 male Japanese quails (one control and 5 experimental groups, n = 10), 3 to 4 wk old. Quails were administered by gavage chlorpyrifos (CPF) for 7 consecutive da at doses of 0.375 mg/kg BW, 0.75 mg/kg BW, 1.5 mg/kg BW, 3 mg/kg BW, and 6 mg/kg BW. Our studies have shown that all doses of CPF significantly inhibited both cholinesterases in brain: AChE from 22.74 to 37.83% and BChE from 19.53 to 61.9%, and that inhibition was dose dependent. Also, CPF has led to an increase in the concentration of MDA, GSH, NO2-, and H2O2 and activity of SOD and MPO. Overall, these results support the hypothesis that CPF causes oxidative stress and inflammatory response. This research was carried out on quails because there is hardly any or not enough data about the neurotoxic effect of CPF and especially about its influence on oxidative stress in birds. This study is highly important because we are witnessing massive avian mortality in certain countries due to pesticides.",
publisher = "Oxford Univ Press, Oxford",
journal = "Poultry Science",
title = "Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail",
volume = "97",
number = "5",
pages = "1564-1571",
doi = "10.3382/ps/pey018"
}

Ćupić Miladinović, D., Borozan, S.,& Ivanović, S.. (2018). Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail. in Poultry Science
Oxford Univ Press, Oxford., 97(5), 1564-1571.
https://doi.org/10.3382/ps/pey018

Ćupić Miladinović D, Borozan S, Ivanović S. Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail. in Poultry Science. 2018;97(5):1564-1571.
doi:10.3382/ps/pey018 .

Ćupić Miladinović, Dejana, Borozan, Sunčica, Ivanović, Saša, "Involvement of cholinesterases in oxidative stress induced by chlorpyrifos in the brain of Japanese quail" in Poultry Science, 97, no. 5 (2018):1564-1571,
https://doi.org/10.3382/ps/pey018 . .

TY  - JOUR
AU  - Bošnjak-Neumuller, Jasna
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Vukovic-Gacić, Branka
AU  - Dajic-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knežević-Vukčević, Jelena
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1473
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).
PB  - Univ Karachi, Karachi
T2  - Pakistan Journal of Pharmaceutical Sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
VL  - 30
IS  - 2
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumuller, Jasna and Radaković, Milena and Đelić, Ninoslav and Vukovic-Gacić, Branka and Dajic-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knežević-Vukčević, Jelena and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).",
publisher = "Univ Karachi, Karachi",
journal = "Pakistan Journal of Pharmaceutical Sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
volume = "30",
number = "2",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumuller, J., Radaković, M., Đelić, N., Vukovic-Gacić, B., Dajic-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knežević-Vukčević, J., Spremo-Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan Journal of Pharmaceutical Sciences
Univ Karachi, Karachi., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumuller J, Radaković M, Đelić N, Vukovic-Gacić B, Dajic-Stevanović Z, Kolarević S, Misić D, Stanković M, Knežević-Vukčević J, Spremo-Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan Journal of Pharmaceutical Sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumuller, Jasna, Radaković, Milena, Đelić, Ninoslav, Vukovic-Gacić, Branka, Dajic-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knežević-Vukčević, Jelena, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan Journal of Pharmaceutical Sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Čabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1501
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
VL  - 2017
SP  - 8759764
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Čabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
volume = "2017",
pages = "8759764",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S., Čabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V.,& Spremo-Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2017, 8759764.
https://doi.org/10.1155/2017/8759764

Živković L, Borozan S, Čabarkapa A, Topalović D, Ciptasari U, Bajić V, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;2017:8759764.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica, Čabarkapa, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan, Spremo-Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity, 2017 (2017):8759764,
https://doi.org/10.1155/2017/8759764 . .

TY  - CONF
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Saša
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2717
AB  - The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.
C3  - 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016
T1  - Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline
IS  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2717
ER  - 

@conference{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Saša and Spremo-Potparević, Biljana and Živković, Lada and Stanimirović, Zoran",
year = "2016",
abstract = "The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.",
journal = "3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016",
title = "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline",
number = "71",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2717"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Spremo-Potparević, B., Živković, L.,& Stanimirović, Z.. (2016). Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016(71).
https://hdl.handle.net/21.15107/rcub_veterinar_2717

Radaković M, Borozan S, Đelić N, Ivanović S, Spremo-Potparević B, Živković L, Stanimirović Z. Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016. 2016;(71):null-71.
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Saša, Spremo-Potparević, Biljana, Živković, Lada, Stanimirović, Zoran, "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline" in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016, no. 71 (2016),
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1429
AB  - Context • Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective • The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design • The research team designed a pilot study. Setting •The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants • Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention • Four concentrations of the CS extract— 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL—were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures • Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A—undamaged cells with no tail (<5% damaged DNA); (2) class B—low-level damage (5%-20%); (3) class C—medium-level damage (20%-40%); (4) class D—high-level damage (40%-95%), and (5) class E—total destruction (>95%). Results • The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-μg/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions • The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
PB  - Innovision Communications, Aliso Viejo
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1429
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan and Đelić, Ninoslav and Spremo-Potparević, Biljana",
year = "2016",
abstract = "Context • Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective • The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design • The research team designed a pilot study. Setting •The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants • Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention • Four concentrations of the CS extract— 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL—were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures • Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A—undamaged cells with no tail (<5% damaged DNA); (2) class B—low-level damage (5%-20%); (3) class C—medium-level damage (20%-40%); (4) class D—high-level damage (40%-95%), and (5) class E—total destruction (>95%). Results • The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-μg/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions • The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
publisher = "Innovision Communications, Aliso Viejo",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1429"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V., Đelić, N.,& Spremo-Potparević, B.. (2016). Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay. in Alternative Therapies in Health and Medicine
Innovision Communications, Aliso Viejo., 22, 24-31.
https://hdl.handle.net/21.15107/rcub_veterinar_1429

Vasiljević J, Živković L, Čabarkapa A, Bajić V, Đelić N, Spremo-Potparević B. Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_veterinar_1429 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan, Đelić, Ninoslav, Spremo-Potparević, Biljana, "Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_veterinar_1429 .

TY  - JOUR
AU  - Dimitrijević, Blagoje
AU  - Jović, Slavoljub
AU  - Ostojić-Andrić, Dušica
AU  - Savić, Mila
AU  - Becskei, Zsolt
AU  - Davidović, Vesna
AU  - Joksimović-Todorović, Mirjana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1321
AB  - The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols.
AB  - Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters
T1  - Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara
VL  - 32
IS  - 4
SP  - 369
EP  - 381
DO  - 10.2298/bah1604369D
ER  - 

@article{
author = "Dimitrijević, Blagoje and Jović, Slavoljub and Ostojić-Andrić, Dušica and Savić, Mila and Becskei, Zsolt and Davidović, Vesna and Joksimović-Todorović, Mirjana",
year = "2016",
abstract = "The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols., Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters, Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara",
volume = "32",
number = "4",
pages = "369-381",
doi = "10.2298/bah1604369D"
}

Dimitrijević, B., Jović, S., Ostojić-Andrić, D., Savić, M., Becskei, Z., Davidović, V.,& Joksimović-Todorović, M.. (2016). Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(4), 369-381.
https://doi.org/10.2298/bah1604369D

Dimitrijević B, Jović S, Ostojić-Andrić D, Savić M, Becskei Z, Davidović V, Joksimović-Todorović M. Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry. 2016;32(4):369-381.
doi:10.2298/bah1604369D .

Dimitrijević, Blagoje, Jović, Slavoljub, Ostojić-Andrić, Dušica, Savić, Mila, Becskei, Zsolt, Davidović, Vesna, Joksimović-Todorović, Mirjana, "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):369-381,
https://doi.org/10.2298/bah1604369D . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Zlatkovic-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jancić, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1418
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright
PB  - Wiley-Blackwell, Hoboken
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Čabarkapa, Andrea and Živković, Lada and Borozan, Sunčica and Zlatkovic-Svenda, Mirjana and Dekanski, Dragana and Jancić, Ivan and Radak-Perović, Marija and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Čabarkapa, A., Živković, L., Borozan, S., Zlatkovic-Svenda, M., Dekanski, D., Jancić, I., Radak-Perović, M., Bajić, V.,& Spremo-Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research
Wiley-Blackwell, Hoboken., 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Čabarkapa A, Živković L, Borozan S, Zlatkovic-Svenda M, Dekanski D, Jancić I, Radak-Perović M, Bajić V, Spremo-Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Čabarkapa, Andrea, Živković, Lada, Borozan, Sunčica, Zlatkovic-Svenda, Mirjana, Dekanski, Dragana, Jancić, Ivan, Radak-Perović, Marija, Bajić, Vladan, Spremo-Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Zlatović, Mario V.
AU  - Stojanović, Srđan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1387
AB  - We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.
PB  - Springer, New York
T2  - Journal of Biological Inorganic Chemistry
T1  - Anion-pi interactions in complexes of proteins and halogen-containing amino acids
VL  - 21
IS  - 3
SP  - 357
EP  - 368
DO  - 10.1007/s00775-016-1346-y
ER  - 

@article{
author = "Borozan, Sunčica and Zlatović, Mario V. and Stojanović, Srđan",
year = "2016",
abstract = "We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.",
publisher = "Springer, New York",
journal = "Journal of Biological Inorganic Chemistry",
title = "Anion-pi interactions in complexes of proteins and halogen-containing amino acids",
volume = "21",
number = "3",
pages = "357-368",
doi = "10.1007/s00775-016-1346-y"
}

Borozan, S., Zlatović, M. V.,& Stojanović, S.. (2016). Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry
Springer, New York., 21(3), 357-368.
https://doi.org/10.1007/s00775-016-1346-y

Borozan S, Zlatović MV, Stojanović S. Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry. 2016;21(3):357-368.
doi:10.1007/s00775-016-1346-y .

Borozan, Sunčica, Zlatović, Mario V., Stojanović, Srđan, "Anion-pi interactions in complexes of proteins and halogen-containing amino acids" in Journal of Biological Inorganic Chemistry, 21, no. 3 (2016):357-368,
https://doi.org/10.1007/s00775-016-1346-y . .

TY  - JOUR
AU  - Žukovec-Topalović, Dijana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1177
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P < 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
VL  - 2015
SP  - 762192
DO  - 10.1155/2015/762192
ER  - 

@article{
author = "Žukovec-Topalović, Dijana and Živković, Lada and Čabarkapa, Andrea and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P < 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
volume = "2015",
pages = "762192",
doi = "10.1155/2015/762192"
}

Žukovec-Topalović, D., Živković, L., Čabarkapa, A., Đelić, N., Bajić, V., Dekanski, D.,& Spremo-Potparević, B.. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2015, 762192.
https://doi.org/10.1155/2015/762192

Žukovec-Topalović D, Živković L, Čabarkapa A, Đelić N, Bajić V, Dekanski D, Spremo-Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity. 2015;2015:762192.
doi:10.1155/2015/762192 .

Žukovec-Topalović, Dijana, Živković, Lada, Čabarkapa, Andrea, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Spremo-Potparević, Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" in Oxidative Medicine and Cellular Longevity, 2015 (2015):762192,
https://doi.org/10.1155/2015/762192 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1197
AB  - Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicology in Vitro
T1  - Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro
VL  - 29
IS  - 1
SP  - 27
EP  - 33
DO  - 10.1016/j.tiv.2014.08.001
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Spremo-Potparević, Biljana and Živković, Lada and Bajić, Vladan and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology in Vitro",
title = "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro",
volume = "29",
number = "1",
pages = "27-33",
doi = "10.1016/j.tiv.2014.08.001"
}

Đelić, N., Radaković, M., Spremo-Potparević, B., Živković, L., Bajić, V., Stevanović, J.,& Stanimirović, Z.. (2015). Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology in Vitro
Pergamon-Elsevier Science Ltd, Oxford., 29(1), 27-33.
https://doi.org/10.1016/j.tiv.2014.08.001

Đelić N, Radaković M, Spremo-Potparević B, Živković L, Bajić V, Stevanović J, Stanimirović Z. Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology in Vitro. 2015;29(1):27-33.
doi:10.1016/j.tiv.2014.08.001 .

Đelić, Ninoslav, Radaković, Milena, Spremo-Potparević, Biljana, Živković, Lada, Bajić, Vladan, Stevanović, Jevrosima, Stanimirović, Zoran, "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro" in Toxicology in Vitro, 29, no. 1 (2015):27-33,
https://doi.org/10.1016/j.tiv.2014.08.001 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Čabarkapa, Andrea
AU  - Jović, Slavoljub
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1212
AB  - Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress.
AB  - Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Genotoxic potential of nonsteroidal hormones
T1  - Genotoksični potencijal nesteroidnih hormona
VL  - 69
IS  - 3-4
SP  - 245
EP  - 257
DO  - 10.2298/VETGL1504245T
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Čabarkapa, Andrea and Jović, Slavoljub and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress., Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Genotoxic potential of nonsteroidal hormones, Genotoksični potencijal nesteroidnih hormona",
volume = "69",
number = "3-4",
pages = "245-257",
doi = "10.2298/VETGL1504245T"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V., Čabarkapa, A., Jović, S.,& Spremo-Potparević, B.. (2015). Genotoxic potential of nonsteroidal hormones. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 245-257.
https://doi.org/10.2298/VETGL1504245T

Topalović D, Živković L, Đelić N, Bajić V, Čabarkapa A, Jović S, Spremo-Potparević B. Genotoxic potential of nonsteroidal hormones. in Veterinarski Glasnik. 2015;69(3-4):245-257.
doi:10.2298/VETGL1504245T .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Čabarkapa, Andrea, Jović, Slavoljub, Spremo-Potparević, Biljana, "Genotoxic potential of nonsteroidal hormones" in Veterinarski Glasnik, 69, no. 3-4 (2015):245-257,
https://doi.org/10.2298/VETGL1504245T . .

TY  - JOUR
AU  - Petrović, Bojana
AU  - Drekić, Dmitar
AU  - Zorić, Zoran
AU  - Jović, Slavoljub
AU  - Nikolić, Milica
AU  - Ćupić Miladinović, Dejana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1208
AB  - The structure of heterochromatin protects genome from undesirable recombinations, while estrogen and luteinizing-releasing hormone (LHRH) cause changes in structure of chromatin. The objective of this study was to prove the connection of neonatally applied estro­gen and LHRH, with shown effects on chromatin in hippocampus of adult male rats. Stereological results have shown that total surface of neuron area in estrogen treated animals significantly reduced in regard to the control group as well as groups treated with LHRH. The number of heterochromatin granules in either estrogen or LHRH treated groups significantly increased, while in the last fourth group treated with both estrogen and LHRH the difference is even more expressed, both in regard to the control group and oth­er groups.
AB  - Struktura heterohromatina štiti genom od neželjenih rekombinacija, dok estrogen i luteinizirajući-oslobađajući hormon (LHRH) uzrokuju promene u samoj strukturi hromatina. Studija je napravljena sa ciljem dokazivanja povezanosti neonatalno aplikovanog estrogena i LHRH, sa ispoljenim efektima na hromatinu u hipokampusu odraslih mužjaka pacova. Stereološki rezultati su pokazali da je ukupna površina neurona kod životinja tretiranih estrogenom značajno smanjena u odnosu na kontrolnu i grupe tretirane LHRH. Broj granula heterohromatina u grupama tretiranim estrogenom i LHRH je primetno povećan, dok je u poslednjoj, četvrtoj grupi tretiranoj i estrogenom i LHRH ova razlika još izraženija, kako u odnosu na kontrolnu, tako i u odnosu na ostale grupe.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus
T1  - Efekti estrogena i luteinizirajućeg - oslobađajućeg hormona na hromatin u CA3 regionu hipokampusa mužjaka pacova
VL  - 69
IS  - 3-4
SP  - 163
EP  - 175
DO  - 10.2298/VETGL1504163P
ER  - 

@article{
author = "Petrović, Bojana and Drekić, Dmitar and Zorić, Zoran and Jović, Slavoljub and Nikolić, Milica and Ćupić Miladinović, Dejana",
year = "2015",
abstract = "The structure of heterochromatin protects genome from undesirable recombinations, while estrogen and luteinizing-releasing hormone (LHRH) cause changes in structure of chromatin. The objective of this study was to prove the connection of neonatally applied estro­gen and LHRH, with shown effects on chromatin in hippocampus of adult male rats. Stereological results have shown that total surface of neuron area in estrogen treated animals significantly reduced in regard to the control group as well as groups treated with LHRH. The number of heterochromatin granules in either estrogen or LHRH treated groups significantly increased, while in the last fourth group treated with both estrogen and LHRH the difference is even more expressed, both in regard to the control group and oth­er groups., Struktura heterohromatina štiti genom od neželjenih rekombinacija, dok estrogen i luteinizirajući-oslobađajući hormon (LHRH) uzrokuju promene u samoj strukturi hromatina. Studija je napravljena sa ciljem dokazivanja povezanosti neonatalno aplikovanog estrogena i LHRH, sa ispoljenim efektima na hromatinu u hipokampusu odraslih mužjaka pacova. Stereološki rezultati su pokazali da je ukupna površina neurona kod životinja tretiranih estrogenom značajno smanjena u odnosu na kontrolnu i grupe tretirane LHRH. Broj granula heterohromatina u grupama tretiranim estrogenom i LHRH je primetno povećan, dok je u poslednjoj, četvrtoj grupi tretiranoj i estrogenom i LHRH ova razlika još izraženija, kako u odnosu na kontrolnu, tako i u odnosu na ostale grupe.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus, Efekti estrogena i luteinizirajućeg - oslobađajućeg hormona na hromatin u CA3 regionu hipokampusa mužjaka pacova",
volume = "69",
number = "3-4",
pages = "163-175",
doi = "10.2298/VETGL1504163P"
}

Petrović, B., Drekić, D., Zorić, Z., Jović, S., Nikolić, M.,& Ćupić Miladinović, D.. (2015). Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 163-175.
https://doi.org/10.2298/VETGL1504163P

Petrović B, Drekić D, Zorić Z, Jović S, Nikolić M, Ćupić Miladinović D. Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus. in Veterinarski Glasnik. 2015;69(3-4):163-175.
doi:10.2298/VETGL1504163P .

Petrović, Bojana, Drekić, Dmitar, Zorić, Zoran, Jović, Slavoljub, Nikolić, Milica, Ćupić Miladinović, Dejana, "Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus" in Veterinarski Glasnik, 69, no. 3-4 (2015):163-175,
https://doi.org/10.2298/VETGL1504163P . .

TY  - JOUR
AU  - Ćupić, Vitomir
AU  - Jović, Slavoljub
AU  - Ristić, Gordana
AU  - Vakanjac, Slobodanka
AU  - Dimitrijević, Blagoje
AU  - Ćupić Miladinović, Dejana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1209
AB  - sows, induction of partus, prostaglandin, oxyitocin, duration of partus, number of born piglets, period between two expulsions of piglets.
AB  - Cilj ovog rada bio je da se ispita uticaj kombinovane primene prostaglandina i oksitocina na dužinu trajanja prašenja, broj živorođene prasadi i vremenski interval između ekspulzije dva praseta kod krmača. Ogled je izveden na 133 gravidne krmače, rase danski landras-veliki jorkšir, koje su (u zavisnosti od dana indukovanja partusa - 112, 113. i 114. dan graviditeta) podeljene u 3 grupe. U okviru svake grupe jedinke su podeljene u tri podgrupe i njima je primenjivan sintetički analog prostaglandina PGF2-alfa (dinoprost-DINOLYTIC) u dozi od 10 mg (ekv. 2 ml preparata)/životinja (I-podgrupa) sam ili u kombinaciji sa jednokratnom (nakon rađanja petog praseta) (II-pod­grupa), odnosno dvokratnom (nakon rađanja petog i desetog prase­ta) (III-podgrupa) aplikacijom oksitocina (OXYTOKEL) u dozi od 20 i.j. (ekv. 2 ml preparata/životinja (jednokratna aplikacija), odnosno 20 i.j.+ 15 i.j. (ekv. 2 ml + 1,5 ml preparata/životinja), (dvokratna aplikacija). Dobijeni rezultati pokazali su da je kod jedinki tretiranih samo prostaglandinom najduže prašenje (6,41 ± 2,20 h) trajalo kod krmača kojima je ovaj lek aplikovan 112. dana graviditeta, a najkraće (4,56 ± 1,30 h) kod jedinki tretiranih 114. dana graviditeta. Kod krmača kojima je (pored prostaglandina) aplikovan jednokratno oksitocin, najduže prašenje (6,38 ± 1,30) je zabeleženo kod jedinki tretiranih 113. dana graviditeta, a najkraće (5,26 ± 1,20) kod onih koje su tretirane 114. dana graviditeta. Kada su krmače (pored prostaglandina) tretirane dvokratno oksitocinom, najduže prašenje (7,17 ± 2,30) je utvrđeno u grupi tretiranoj 112. dana graviditeta, a najkraće (5,10 ± 1,20) kod jedinki tretiranih 113. dana graviditeta. Ukupno posmatrano, najduže prašenje (7,17 ± 2,30) je trajalo kod jedinki koje su (pored prostaglandina) dobile dvokratno oksitocin, a najkraće (4,56 ± 1,30), kod jedinki koje su tretirane samo prostaglandinom. Najveći broj živorođene prasadi (358) zabeležen je kod krmača kojima je aplikovan prostaglandin 114. dana graviditeta u kombinaciji sa jednokratnom aplikacijom oksitocina, a najmanji (250) kod krmača kojima je aplikovan prostaglandin 112. dana graviditeta u kombinaciji sa dvokratnom aplikacijom oksitocina. Najduži interval (0,22 ± 0,06) između ekspulzije dva praseta zabeležen je kod krmača kojima je indukovan partus 112. dana graviditeta u kombinaciji sa dvokratnom aplikacijom oksitocina, a najkraći (0,11 ± 0,03) kod krmača kojima je indukcija prašenja vršena 114. dana graviditeta, bez naknadne primene oksitocina.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The effect of combined use of prostaglandin and oxytocin in sows, on the duration of partus, number of pigs and period between two expulsions of piglets
T1  - Efekat kombinovane primene prostaglandina i oksitocina na dužinu trajanja partusa, broj prasadi i vreme između ekspulzije dva praseta kod krmača
VL  - 69
IS  - 3-4
SP  - 177
EP  - 193
DO  - 10.2298/VETGL1504177C
ER  - 

@article{
author = "Ćupić, Vitomir and Jović, Slavoljub and Ristić, Gordana and Vakanjac, Slobodanka and Dimitrijević, Blagoje and Ćupić Miladinović, Dejana",
year = "2015",
abstract = "sows, induction of partus, prostaglandin, oxyitocin, duration of partus, number of born piglets, period between two expulsions of piglets., Cilj ovog rada bio je da se ispita uticaj kombinovane primene prostaglandina i oksitocina na dužinu trajanja prašenja, broj živorođene prasadi i vremenski interval između ekspulzije dva praseta kod krmača. Ogled je izveden na 133 gravidne krmače, rase danski landras-veliki jorkšir, koje su (u zavisnosti od dana indukovanja partusa - 112, 113. i 114. dan graviditeta) podeljene u 3 grupe. U okviru svake grupe jedinke su podeljene u tri podgrupe i njima je primenjivan sintetički analog prostaglandina PGF2-alfa (dinoprost-DINOLYTIC) u dozi od 10 mg (ekv. 2 ml preparata)/životinja (I-podgrupa) sam ili u kombinaciji sa jednokratnom (nakon rađanja petog praseta) (II-pod­grupa), odnosno dvokratnom (nakon rađanja petog i desetog prase­ta) (III-podgrupa) aplikacijom oksitocina (OXYTOKEL) u dozi od 20 i.j. (ekv. 2 ml preparata/životinja (jednokratna aplikacija), odnosno 20 i.j.+ 15 i.j. (ekv. 2 ml + 1,5 ml preparata/životinja), (dvokratna aplikacija). Dobijeni rezultati pokazali su da je kod jedinki tretiranih samo prostaglandinom najduže prašenje (6,41 ± 2,20 h) trajalo kod krmača kojima je ovaj lek aplikovan 112. dana graviditeta, a najkraće (4,56 ± 1,30 h) kod jedinki tretiranih 114. dana graviditeta. Kod krmača kojima je (pored prostaglandina) aplikovan jednokratno oksitocin, najduže prašenje (6,38 ± 1,30) je zabeleženo kod jedinki tretiranih 113. dana graviditeta, a najkraće (5,26 ± 1,20) kod onih koje su tretirane 114. dana graviditeta. Kada su krmače (pored prostaglandina) tretirane dvokratno oksitocinom, najduže prašenje (7,17 ± 2,30) je utvrđeno u grupi tretiranoj 112. dana graviditeta, a najkraće (5,10 ± 1,20) kod jedinki tretiranih 113. dana graviditeta. Ukupno posmatrano, najduže prašenje (7,17 ± 2,30) je trajalo kod jedinki koje su (pored prostaglandina) dobile dvokratno oksitocin, a najkraće (4,56 ± 1,30), kod jedinki koje su tretirane samo prostaglandinom. Najveći broj živorođene prasadi (358) zabeležen je kod krmača kojima je aplikovan prostaglandin 114. dana graviditeta u kombinaciji sa jednokratnom aplikacijom oksitocina, a najmanji (250) kod krmača kojima je aplikovan prostaglandin 112. dana graviditeta u kombinaciji sa dvokratnom aplikacijom oksitocina. Najduži interval (0,22 ± 0,06) između ekspulzije dva praseta zabeležen je kod krmača kojima je indukovan partus 112. dana graviditeta u kombinaciji sa dvokratnom aplikacijom oksitocina, a najkraći (0,11 ± 0,03) kod krmača kojima je indukcija prašenja vršena 114. dana graviditeta, bez naknadne primene oksitocina.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The effect of combined use of prostaglandin and oxytocin in sows, on the duration of partus, number of pigs and period between two expulsions of piglets, Efekat kombinovane primene prostaglandina i oksitocina na dužinu trajanja partusa, broj prasadi i vreme između ekspulzije dva praseta kod krmača",
volume = "69",
number = "3-4",
pages = "177-193",
doi = "10.2298/VETGL1504177C"
}

Ćupić, V., Jović, S., Ristić, G., Vakanjac, S., Dimitrijević, B.,& Ćupić Miladinović, D.. (2015). The effect of combined use of prostaglandin and oxytocin in sows, on the duration of partus, number of pigs and period between two expulsions of piglets. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 177-193.
https://doi.org/10.2298/VETGL1504177C

Ćupić V, Jović S, Ristić G, Vakanjac S, Dimitrijević B, Ćupić Miladinović D. The effect of combined use of prostaglandin and oxytocin in sows, on the duration of partus, number of pigs and period between two expulsions of piglets. in Veterinarski Glasnik. 2015;69(3-4):177-193.
doi:10.2298/VETGL1504177C .

Ćupić, Vitomir, Jović, Slavoljub, Ristić, Gordana, Vakanjac, Slobodanka, Dimitrijević, Blagoje, Ćupić Miladinović, Dejana, "The effect of combined use of prostaglandin and oxytocin in sows, on the duration of partus, number of pigs and period between two expulsions of piglets" in Veterinarski Glasnik, 69, no. 3-4 (2015):177-193,
https://doi.org/10.2298/VETGL1504177C . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1827
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicology
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicology",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicology, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1249
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Zivković, L.
AU  - Stojanović, S.
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1310
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Zivković, L. and Stojanović, S. and Milanović-Čabarkapa, Mirjana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Čabarkapa, A., Borozan, S., Zivković, L., Stojanović, S., Milanović-Čabarkapa, M., Bajić, V.,& Spremo-Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Čabarkapa A, Borozan S, Zivković L, Stojanović S, Milanović-Čabarkapa M, Bajić V, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Čabarkapa, Andrea, Borozan, Sunčica, Zivković, L., Stojanović, S., Milanović-Čabarkapa, Mirjana, Bajić, Vladan, Spremo-Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .