Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge University Press., 87(4), 429-435.
https://hdl.handle.net/21.15107/rcub_veterinar_1976

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
https://hdl.handle.net/21.15107/rcub_veterinar_1976 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://hdl.handle.net/21.15107/rcub_veterinar_1976 .

TY  - CONF
AU  - Ledina, Tijana
AU  - Đorđević, Jasna
AU  - Babić, Milijana
AU  - Bulajić, Snežana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2839
AB  - Туберкулоза представља водећи узрок смрти од инфективних обољења у свету, са 
1,7 милиона смртних случајева у 2017.години. У периоду 2013-2017. забележен је 
пораст броја случајева туберкулозе људи, у којима је као узрочник идентификован 
Mycobacterium bovis, најчешћи узрочник туберкулозе преживара. Сматра се да је 
стваран број људи оболелих од туберкулозе узроковане са М. bovis подцењен, 
нарочито у подручјима где је туберкулоза говеда ендемично обољење или се 
конзумирају сирово млеко и производи од млека и када постоји директан контакт 
са оболелим животињама.
Зоонотска туберкулоза изазвана са M. bovis представља превасходно обољење 
преносиво храном, мада најновији извештаји наглашавају и могућност аерогене 
секундарне трансмисије M. bovis са човека на човека. Ћелије M. bovis се у случају 
обољевања млечних животиња излучују путем млека у високом броју, при чему 
постоји могућност контаминације велике количине збирног млека у 
лактофризима или цистернама. Конзумација сировог млека и производа 
направљених од сировог млека, историјски гледано, представља главни пут 
преноса туберкулозе са животиња на људе. Увођењем пастеризације млека, али и 
спровођењем програма мера здравствене заштите животиња, овај вид обољевања 
од туберкулозе је у развијеним земљама сведен на занемарљиву меру. Млеко 
оболелих животиња може да уђе у ланац хране, у случајевима када оболеле 
животиње на дају позитивну реакцију туберкулинизације. У земљама Европе у 
којима је продаја сировог млека дозвољена, млеко мора да потиче од животиња из 
стада слободних од туберкулозе, а на декларацији мора да буде јасно наглашен 
здравствени ризик повезан са конзумацијом сировог млека. Све већа потражња за 
сиревима произведеним од сировог млека, али и скорији случајеви туберкулозе 
људи изазвани конзумацијом сирева од сировог млека, питање зоонотске 
туберкулозе чине и данас актуелним.
У овом прегледном раду биће размотрена епидемиологија зоонотске туберкулозе, 
процена ризика од обољевања при конзумацији сировог млека и производа 
направљених од сировог млека, као и стратегије за спречавање ширења зоонотске 
туберкулозе као обољења преносивог храном
AB  - Tuberculosis is the leading cause of the death from a single infectious agent in the 
World. It is estimated that 1.7 million people died of tuberculosis in 2017. In the 
period 2013-2017. an increase in tuberculosis caused by Mycobacterium bovis, 
most frequent causative agent of ruminant tuberculosis, was reported. However, 
it is estimated that the true number of human tuberculosis caused by M. bovis is 
underestimated, especially in areas with endemic bovine tuberculosis, where 
direct contact between humans and animals exists, or raw milk and raw milk 
products are frequently consumed. Zoonotic tuberculosis is mostly foodborne 
disease, although recent reports highlight the possibility of aerogenic secondary 
transmission of M.bovis between humans. Lactating animals with tuberculosis can 
excrete high numbers of M. bovis cells through milk. Consequently milk from 
these animals can contaminate large quantities of raw milk in cooling tanks and 
cisterns. Historically, consumption of raw milk and raw milk products represents 
the main transmission route between animal and human population. However, 
introduction of control measures and pasteurization has reduced cases of 
foodborne tuberculosis to a minimum in developed countries. Milk from animals 
with tuberculosis can enter the food chain, in cases when animals remain 
undetected by the skin test. In European countries, if selling of raw milk is 
permitted by national legislation, food label must include statement about health 
risks implicated with the raw milk consumption. Increasing demand for the 
artisan raw milk cheeses, as well as recent cases of tuberculosis caused by raw milk 
cheese consumption, also make zoonotic tuberculosis a contemporary food safety 
issue. In this review, zoonotic tuberculosis epidemiology, risk assessment 
correlated with the raw milk and raw milk products consumption, as well as 
strategies for zoonotic tuberculosis prevention as a foodborne disease will be 
discussed.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
T1  - Zoonotska tuberkuloza - šta nam je činiti?
T1  - Zoonotic tuberculosis - what should be done?
SP  - 111
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2839
ER  - 

@conference{
author = "Ledina, Tijana and Đorđević, Jasna and Babić, Milijana and Bulajić, Snežana",
year = "2019",
abstract = "Туберкулоза представља водећи узрок смрти од инфективних обољења у свету, са 
1,7 милиона смртних случајева у 2017.години. У периоду 2013-2017. забележен је 
пораст броја случајева туберкулозе људи, у којима је као узрочник идентификован 
Mycobacterium bovis, најчешћи узрочник туберкулозе преживара. Сматра се да је 
стваран број људи оболелих од туберкулозе узроковане са М. bovis подцењен, 
нарочито у подручјима где је туберкулоза говеда ендемично обољење или се 
конзумирају сирово млеко и производи од млека и када постоји директан контакт 
са оболелим животињама.
Зоонотска туберкулоза изазвана са M. bovis представља превасходно обољење 
преносиво храном, мада најновији извештаји наглашавају и могућност аерогене 
секундарне трансмисије M. bovis са човека на човека. Ћелије M. bovis се у случају 
обољевања млечних животиња излучују путем млека у високом броју, при чему 
постоји могућност контаминације велике количине збирног млека у 
лактофризима или цистернама. Конзумација сировог млека и производа 
направљених од сировог млека, историјски гледано, представља главни пут 
преноса туберкулозе са животиња на људе. Увођењем пастеризације млека, али и 
спровођењем програма мера здравствене заштите животиња, овај вид обољевања 
од туберкулозе је у развијеним земљама сведен на занемарљиву меру. Млеко 
оболелих животиња може да уђе у ланац хране, у случајевима када оболеле 
животиње на дају позитивну реакцију туберкулинизације. У земљама Европе у 
којима је продаја сировог млека дозвољена, млеко мора да потиче од животиња из 
стада слободних од туберкулозе, а на декларацији мора да буде јасно наглашен 
здравствени ризик повезан са конзумацијом сировог млека. Све већа потражња за 
сиревима произведеним од сировог млека, али и скорији случајеви туберкулозе 
људи изазвани конзумацијом сирева од сировог млека, питање зоонотске 
туберкулозе чине и данас актуелним.
У овом прегледном раду биће размотрена епидемиологија зоонотске туберкулозе, 
процена ризика од обољевања при конзумацији сировог млека и производа 
направљених од сировог млека, као и стратегије за спречавање ширења зоонотске 
туберкулозе као обољења преносивог храном, Tuberculosis is the leading cause of the death from a single infectious agent in the 
World. It is estimated that 1.7 million people died of tuberculosis in 2017. In the 
period 2013-2017. an increase in tuberculosis caused by Mycobacterium bovis, 
most frequent causative agent of ruminant tuberculosis, was reported. However, 
it is estimated that the true number of human tuberculosis caused by M. bovis is 
underestimated, especially in areas with endemic bovine tuberculosis, where 
direct contact between humans and animals exists, or raw milk and raw milk 
products are frequently consumed. Zoonotic tuberculosis is mostly foodborne 
disease, although recent reports highlight the possibility of aerogenic secondary 
transmission of M.bovis between humans. Lactating animals with tuberculosis can 
excrete high numbers of M. bovis cells through milk. Consequently milk from 
these animals can contaminate large quantities of raw milk in cooling tanks and 
cisterns. Historically, consumption of raw milk and raw milk products represents 
the main transmission route between animal and human population. However, 
introduction of control measures and pasteurization has reduced cases of 
foodborne tuberculosis to a minimum in developed countries. Milk from animals 
with tuberculosis can enter the food chain, in cases when animals remain 
undetected by the skin test. In European countries, if selling of raw milk is 
permitted by national legislation, food label must include statement about health 
risks implicated with the raw milk consumption. Increasing demand for the 
artisan raw milk cheeses, as well as recent cases of tuberculosis caused by raw milk 
cheese consumption, also make zoonotic tuberculosis a contemporary food safety 
issue. In this review, zoonotic tuberculosis epidemiology, risk assessment 
correlated with the raw milk and raw milk products consumption, as well as 
strategies for zoonotic tuberculosis prevention as a foodborne disease will be 
discussed.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.",
title = "Zoonotska tuberkuloza - šta nam je činiti?, Zoonotic tuberculosis - what should be done?",
pages = "111-112",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2839"
}

Ledina, T., Đorđević, J., Babić, M.,& Bulajić, S.. (2019). Zoonotska tuberkuloza - šta nam je činiti?. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
Banja Luka : Veterinarska komora Republike Srpske., 111-112.
https://hdl.handle.net/21.15107/rcub_veterinar_2839

Ledina T, Đorđević J, Babić M, Bulajić S. Zoonotska tuberkuloza - šta nam je činiti?. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.. 2019;:111-112.
https://hdl.handle.net/21.15107/rcub_veterinar_2839 .

Ledina, Tijana, Đorđević, Jasna, Babić, Milijana, Bulajić, Snežana, "Zoonotska tuberkuloza - šta nam je činiti?" in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019. (2019):111-112,
https://hdl.handle.net/21.15107/rcub_veterinar_2839 .

TY  - CONF
AU  - Bulajić, Snežana
AU  - Ledina, Tijana
AU  - Đorđević, Jasna
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2873
AB  - Функционална храна је новији тренд у исхрани, а односи се на храну која, поред
задовољавања нутритивних потреба, позитивно утиче на здравље потрошача. Сегмент
функционалне хране првенствено се развија услед „технолошког притиска― усмереног на
истраживање могућности тржишног позиционирања иновативних производа, а мање на захтев
самих потрошача. Домаће тржиште функционалне хране је оперативно, али законски
нерегулисано и такво окружење пружа могућност за манипулацију потрошача. Да ли се ситуација
мења на боље увођењем Правилника о прехрамбеним (нутритивним) и здравственим изјавама?
AB  - Functional food is a recent trend in nutrition and refers to the food, that in addition to meeting
nutritional requirements, have a positive effect on consumer health. The functional food sector is
primarily developing due to the "technological pressure" aimed at exploring the possibilities of market positioning of innovative products, much less at the request of consumers themselves. The national functional food market is operational but not regulated by law, and such an environment provides an opportunity for consumer manipulation. Is the situation changing for the better with the introduction of the Regulation on Nutrition and Health Claims made on foods?
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 30. Savetovanje veterinara Srbije, Zlatibor, 12-15. septembar 2019
T1  - Tržište funkcionalne hrane u Srbiji kroz prizmu novih propisa
T1  - Functional food market in Serbia through the prism of new regulation
SP  - 204
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2873
ER  - 

@conference{
author = "Bulajić, Snežana and Ledina, Tijana and Đorđević, Jasna",
year = "2019",
abstract = "Функционална храна је новији тренд у исхрани, а односи се на храну која, поред
задовољавања нутритивних потреба, позитивно утиче на здравље потрошача. Сегмент
функционалне хране првенствено се развија услед „технолошког притиска― усмереног на
истраживање могућности тржишног позиционирања иновативних производа, а мање на захтев
самих потрошача. Домаће тржиште функционалне хране је оперативно, али законски
нерегулисано и такво окружење пружа могућност за манипулацију потрошача. Да ли се ситуација
мења на боље увођењем Правилника о прехрамбеним (нутритивним) и здравственим изјавама?, Functional food is a recent trend in nutrition and refers to the food, that in addition to meeting
nutritional requirements, have a positive effect on consumer health. The functional food sector is
primarily developing due to the "technological pressure" aimed at exploring the possibilities of market positioning of innovative products, much less at the request of consumers themselves. The national functional food market is operational but not regulated by law, and such an environment provides an opportunity for consumer manipulation. Is the situation changing for the better with the introduction of the Regulation on Nutrition and Health Claims made on foods?",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "30. Savetovanje veterinara Srbije, Zlatibor, 12-15. septembar 2019",
title = "Tržište funkcionalne hrane u Srbiji kroz prizmu novih propisa, Functional food market in Serbia through the prism of new regulation",
pages = "204-210",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2873"
}

Bulajić, S., Ledina, T.,& Đorđević, J.. (2019). Tržište funkcionalne hrane u Srbiji kroz prizmu novih propisa. in 30. Savetovanje veterinara Srbije, Zlatibor, 12-15. septembar 2019
Beograd : Srpsko veterinarsko društvo., 204-210.
https://hdl.handle.net/21.15107/rcub_veterinar_2873

Bulajić S, Ledina T, Đorđević J. Tržište funkcionalne hrane u Srbiji kroz prizmu novih propisa. in 30. Savetovanje veterinara Srbije, Zlatibor, 12-15. septembar 2019. 2019;:204-210.
https://hdl.handle.net/21.15107/rcub_veterinar_2873 .

Bulajić, Snežana, Ledina, Tijana, Đorđević, Jasna, "Tržište funkcionalne hrane u Srbiji kroz prizmu novih propisa" in 30. Savetovanje veterinara Srbije, Zlatibor, 12-15. septembar 2019 (2019):204-210,
https://hdl.handle.net/21.15107/rcub_veterinar_2873 .

TY  - JOUR
AU  - Ledina, Tijana
AU  - Golob, Majda
AU  - Đorđević, Jasna
AU  - Magaš, Vladimir
AU  - Čolović Svetlana
AU  - Bulajić, Snežana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1562
AB  - The study was carried out on 110 bacterial isolates obtained from Baird-Parker agar plates (n = 47) and de Man, Rogosa, Sharpe agar plates with added sorbic acid (n = 63), which were analyzed by MALDI-TOF mass spectrometry. Among 47 non-staphylococcal Baird-Parker agar isolates, Macrococcus caseolyticus was the most abundant (n = 37), followed by Enterococcus faecalis (n = 6). Corynebacterium aurimucosum, Corynebacterium glutamicum, Cronobacter sakazakii, and Enterococcus faecium were also identified. Among 63 non-Lactobacillus isolates, 18 isolates were identified as Leuconostoc mesenteroides, 17 as Enterococcus faecalis, 14 as Lactococcus lactis, 5 as Enterococcus durans, 5 as Lactococcus garvieae, 2 as Leuconostoc pseudomesenteroides, and 2 as Pediococcus pentosaceus. Enterococcus faecalis was the only species obtained from both types of media. All isolates were successfully identified to the species level. According to the results of this study, MALDI-TOF mass spectrometry proved to be useful tool for the identification of selected lactic acid bacteria and Baird-Parker agar isolates from artisanal cheeses.
PB  - Springer International Publishing Ag, Cham
T2  - Journal of Consumer Protection and Food Safety
T1  - MALDI-TOF mass spectrometry for the identification of Serbian artisanal cheeses microbiota
VL  - 13
IS  - 3
SP  - 309
EP  - 314
DO  - 10.1007/s00003-018-1164-y
ER  - 

@article{
author = "Ledina, Tijana and Golob, Majda and Đorđević, Jasna and Magaš, Vladimir and Čolović Svetlana and Bulajić, Snežana",
year = "2018",
abstract = "The study was carried out on 110 bacterial isolates obtained from Baird-Parker agar plates (n = 47) and de Man, Rogosa, Sharpe agar plates with added sorbic acid (n = 63), which were analyzed by MALDI-TOF mass spectrometry. Among 47 non-staphylococcal Baird-Parker agar isolates, Macrococcus caseolyticus was the most abundant (n = 37), followed by Enterococcus faecalis (n = 6). Corynebacterium aurimucosum, Corynebacterium glutamicum, Cronobacter sakazakii, and Enterococcus faecium were also identified. Among 63 non-Lactobacillus isolates, 18 isolates were identified as Leuconostoc mesenteroides, 17 as Enterococcus faecalis, 14 as Lactococcus lactis, 5 as Enterococcus durans, 5 as Lactococcus garvieae, 2 as Leuconostoc pseudomesenteroides, and 2 as Pediococcus pentosaceus. Enterococcus faecalis was the only species obtained from both types of media. All isolates were successfully identified to the species level. According to the results of this study, MALDI-TOF mass spectrometry proved to be useful tool for the identification of selected lactic acid bacteria and Baird-Parker agar isolates from artisanal cheeses.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Journal of Consumer Protection and Food Safety",
title = "MALDI-TOF mass spectrometry for the identification of Serbian artisanal cheeses microbiota",
volume = "13",
number = "3",
pages = "309-314",
doi = "10.1007/s00003-018-1164-y"
}

Ledina, T., Golob, M., Đorđević, J., Magaš, V., Čolović Svetlana,& Bulajić, S.. (2018). MALDI-TOF mass spectrometry for the identification of Serbian artisanal cheeses microbiota. in Journal of Consumer Protection and Food Safety
Springer International Publishing Ag, Cham., 13(3), 309-314.
https://doi.org/10.1007/s00003-018-1164-y

Ledina T, Golob M, Đorđević J, Magaš V, Čolović Svetlana, Bulajić S. MALDI-TOF mass spectrometry for the identification of Serbian artisanal cheeses microbiota. in Journal of Consumer Protection and Food Safety. 2018;13(3):309-314.
doi:10.1007/s00003-018-1164-y .

Ledina, Tijana, Golob, Majda, Đorđević, Jasna, Magaš, Vladimir, Čolović Svetlana, Bulajić, Snežana, "MALDI-TOF mass spectrometry for the identification of Serbian artisanal cheeses microbiota" in Journal of Consumer Protection and Food Safety, 13, no. 3 (2018):309-314,
https://doi.org/10.1007/s00003-018-1164-y . .

TY  - CONF
AU  - Đorđević, Jasna
AU  - Ledina, Tijana
AU  - Bulajić, Snežana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2916
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
T1  - Senzorna ocena fermentisanih proizvoda od mleka
SP  - 79
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2916
ER  - 

@conference{
author = "Đorđević, Jasna and Ledina, Tijana and Bulajić, Snežana",
year = "2018",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.",
title = "Senzorna ocena fermentisanih proizvoda od mleka",
pages = "79-80",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2916"
}

Đorđević, J., Ledina, T.,& Bulajić, S.. (2018). Senzorna ocena fermentisanih proizvoda od mleka. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
Banja Luka : Veterinarska komora Republike Srpske., 79-80.
https://hdl.handle.net/21.15107/rcub_veterinar_2916

Đorđević J, Ledina T, Bulajić S. Senzorna ocena fermentisanih proizvoda od mleka. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.. 2018;:79-80.
https://hdl.handle.net/21.15107/rcub_veterinar_2916 .

Đorđević, Jasna, Ledina, Tijana, Bulajić, Snežana, "Senzorna ocena fermentisanih proizvoda od mleka" in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018. (2018):79-80,
https://hdl.handle.net/21.15107/rcub_veterinar_2916 .

TY  - JOUR
AU  - Ledina, Tijana
AU  - Mohar-Lorbeg, Petra
AU  - Golob, Majda
AU  - Đorđević, Jasna
AU  - Bogović-Matijasić, Bojana
AU  - Bulajić, Snežana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1635
AB  - The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses
VL  - 55
IS  - 4
SP  - 1426
EP  - 1434
DO  - 10.1007/s13197-018-3057-6
ER  - 

@article{
author = "Ledina, Tijana and Mohar-Lorbeg, Petra and Golob, Majda and Đorđević, Jasna and Bogović-Matijasić, Bojana and Bulajić, Snežana",
year = "2018",
abstract = "The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses",
volume = "55",
number = "4",
pages = "1426-1434",
doi = "10.1007/s13197-018-3057-6"
}

Ledina, T., Mohar-Lorbeg, P., Golob, M., Đorđević, J., Bogović-Matijasić, B.,& Bulajić, S.. (2018). Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(4), 1426-1434.
https://doi.org/10.1007/s13197-018-3057-6

Ledina T, Mohar-Lorbeg P, Golob M, Đorđević J, Bogović-Matijasić B, Bulajić S. Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore. 2018;55(4):1426-1434.
doi:10.1007/s13197-018-3057-6 .

Ledina, Tijana, Mohar-Lorbeg, Petra, Golob, Majda, Đorđević, Jasna, Bogović-Matijasić, Bojana, Bulajić, Snežana, "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses" in Journal of Food Science and Technology-Mysore, 55, no. 4 (2018):1426-1434,
https://doi.org/10.1007/s13197-018-3057-6 . .

TY  - JOUR
AU  - Ledina, Tijana
AU  - Bulajić, Snežana
AU  - Đorđević, Jasna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2168
AB  - Rezistencija na antibiotike predstavlja rastući problem koji je Svetska
zdravstvena organizacija proglasila jednom od najvećih pretnji za globalno zdravlje
ljudi. Lanac hrane jedan je od najvažnijih puteva prenošenja i širenja rezistencije
na antibiotike između populacije rezistentnih i populacije osetljivih komensalnih
i/ili patogenih mikroorganizama. Rezistencija na antibiotike može biti urođena, ili
stečena putem mutacija ili lateralnog transfera gena. Sa aspekta širenja rezistencije
značajna je samo rezistencija stečena lateralnim transferom gena. Postoje brojne
metode za detekciju i određivanje prirode rezistencije na antibiotike kod bakterija
izolovanih iz hrane. Neophodno je da metode budu standardizovane i da obezbeđuju
konzistentnost i doslednost dobijenih rezultata. Za detekciju fenotipske rezistencije
kod bakterija izolovanih iz hrane koriste se metode kojima se određuje minimalna
inhibitorna koncentracija antibiotika. U njih spadaju metod mikrodilucije, metod
dilucije u agaru i E-testovi. Kvalitativne i semikvantitativne metode koje se često
koriste kod kliničkih izolata, nisu prigodne za ispitivanje rezistencije na antibiotike
kod mikroorganizama izolovanih iz hrane. Kod mikroorganizama kod kojih se
utvrdi prisustvo fenotipske rezistencije na antibiotike, određuje se prisustvo gena za
rezistenciju. Mikroorganizmi kod kojih se dokaže prisustvo genetskih determinanti
koje su povezane sa stečenom rezistencijom na antibiotike, predstavljaju rizik za
diseminaciju rezistencije i među osetljivom populacijom. Mikroorganizmi koji imaju
komercijalnu upotrebu, ne bi trebalo da poseduju genetske determinante prenosive
rezistencije na antibiotike.
AB  - Resistance to antibiotics is a growing problem that the World Health
Organization has declared one of the biggest threats to global health. The food
chain is one of the most important ways of transmitting and spreading resistance
to antibiotics between the population of resistant and populated by sensitive
commensal and / or pathogenic microorganisms. Resistance to antibiotics can be
inborn, or acquired by mutation or lateral gene transfer. From the aspect of the
spread of resistance, only resistance acquired by the lateral transfer of the gene
is significant. There are numerous methods for detecting and determining the
nature of antibiotic resistance in bacteria isolated from food. The methods must
be standardized and ensure the consistency of the obtained results. Methods for
determining the minimum inhibitory concentration of antibiotics are used to detect
phenotypic resistance in bacteria isolated from food. They include a microdilution
method, an agar dilution method, and an E-test. Qualitative and semi-quantitative
methods commonly used in clinical isolates are not suitable for antibiotic resistance
testing in food-isolated microorganisms. In the case of microorganisms with
detected presence of phenotypic resistance to antibiotics, the presence of the
resistance gene is determined. Microorganisms evidenced by the presence of genetic
determinants associated with acquired resistance to antibiotics represent a risk of
resistance dissemination among the susceptible populations. Commercially used
microorganisms should not possess genetic determinants of transferable antibiotic
resistance.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Metode za određivanje antimikrobne rezistencije kod mikroorganizama u hrani
T1  - Methods for the antibiotic resistance detection in microorganisms isolated from food
VL  - 18
IS  - 1
SP  - 207
EP  - 224
DO  - 10.7251/VETJ1801207L
ER  - 

@article{
author = "Ledina, Tijana and Bulajić, Snežana and Đorđević, Jasna",
year = "2018",
abstract = "Rezistencija na antibiotike predstavlja rastući problem koji je Svetska
zdravstvena organizacija proglasila jednom od najvećih pretnji za globalno zdravlje
ljudi. Lanac hrane jedan je od najvažnijih puteva prenošenja i širenja rezistencije
na antibiotike između populacije rezistentnih i populacije osetljivih komensalnih
i/ili patogenih mikroorganizama. Rezistencija na antibiotike može biti urođena, ili
stečena putem mutacija ili lateralnog transfera gena. Sa aspekta širenja rezistencije
značajna je samo rezistencija stečena lateralnim transferom gena. Postoje brojne
metode za detekciju i određivanje prirode rezistencije na antibiotike kod bakterija
izolovanih iz hrane. Neophodno je da metode budu standardizovane i da obezbeđuju
konzistentnost i doslednost dobijenih rezultata. Za detekciju fenotipske rezistencije
kod bakterija izolovanih iz hrane koriste se metode kojima se određuje minimalna
inhibitorna koncentracija antibiotika. U njih spadaju metod mikrodilucije, metod
dilucije u agaru i E-testovi. Kvalitativne i semikvantitativne metode koje se često
koriste kod kliničkih izolata, nisu prigodne za ispitivanje rezistencije na antibiotike
kod mikroorganizama izolovanih iz hrane. Kod mikroorganizama kod kojih se
utvrdi prisustvo fenotipske rezistencije na antibiotike, određuje se prisustvo gena za
rezistenciju. Mikroorganizmi kod kojih se dokaže prisustvo genetskih determinanti
koje su povezane sa stečenom rezistencijom na antibiotike, predstavljaju rizik za
diseminaciju rezistencije i među osetljivom populacijom. Mikroorganizmi koji imaju
komercijalnu upotrebu, ne bi trebalo da poseduju genetske determinante prenosive
rezistencije na antibiotike., Resistance to antibiotics is a growing problem that the World Health
Organization has declared one of the biggest threats to global health. The food
chain is one of the most important ways of transmitting and spreading resistance
to antibiotics between the population of resistant and populated by sensitive
commensal and / or pathogenic microorganisms. Resistance to antibiotics can be
inborn, or acquired by mutation or lateral gene transfer. From the aspect of the
spread of resistance, only resistance acquired by the lateral transfer of the gene
is significant. There are numerous methods for detecting and determining the
nature of antibiotic resistance in bacteria isolated from food. The methods must
be standardized and ensure the consistency of the obtained results. Methods for
determining the minimum inhibitory concentration of antibiotics are used to detect
phenotypic resistance in bacteria isolated from food. They include a microdilution
method, an agar dilution method, and an E-test. Qualitative and semi-quantitative
methods commonly used in clinical isolates are not suitable for antibiotic resistance
testing in food-isolated microorganisms. In the case of microorganisms with
detected presence of phenotypic resistance to antibiotics, the presence of the
resistance gene is determined. Microorganisms evidenced by the presence of genetic
determinants associated with acquired resistance to antibiotics represent a risk of
resistance dissemination among the susceptible populations. Commercially used
microorganisms should not possess genetic determinants of transferable antibiotic
resistance.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Metode za određivanje antimikrobne rezistencije kod mikroorganizama u hrani, Methods for the antibiotic resistance detection in microorganisms isolated from food",
volume = "18",
number = "1",
pages = "207-224",
doi = "10.7251/VETJ1801207L"
}

Ledina, T., Bulajić, S.,& Đorđević, J.. (2018). Metode za određivanje antimikrobne rezistencije kod mikroorganizama u hrani. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 18(1), 207-224.
https://doi.org/10.7251/VETJ1801207L

Ledina T, Bulajić S, Đorđević J. Metode za određivanje antimikrobne rezistencije kod mikroorganizama u hrani. in Veterinarski žurnal Republike Srpske. 2018;18(1):207-224.
doi:10.7251/VETJ1801207L .

Ledina, Tijana, Bulajić, Snežana, Đorđević, Jasna, "Metode za određivanje antimikrobne rezistencije kod mikroorganizama u hrani" in Veterinarski žurnal Republike Srpske, 18, no. 1 (2018):207-224,
https://doi.org/10.7251/VETJ1801207L . .

TY  - THES
AU  - Ledina, Tijana
PY  - 2018
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50763023
UR  - http://nardus.mpn.gov.rs/123456789/10538
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/47
AB  - Food chain may represent a possible route for the transmission of antibiotic resistant bacteria between human and animal populations. Commensal bacteria originating from food-producing animals can contaminate food and by that means become a part of the complex food microbiota. Commensal bacteria can also serve as reservoirs for the antibiotic resistance genes. Tetracycline resistance in commensal bacteria is well documented and is coded with transmissible genes. Therefore, it is recognized as a model for the monitoring of antibiotic resistance ecology. Homolj, Zlatar and Sjenica cheese have protected geographical indication on the national level. As Homolj, Zlatar and Sjenica cheese are raw milk cheeses produced in the traditional manner, LAB originating from the raw milk and the environment constitute an important part of Serbian traditional cheeses’ microbiota. There are many data in the scientific literature that LAB can carry transmissible antibiotic resistance genes. The aims of this doctoral dissertation were to: characterize biodiversity of traditional Serbian cheeses; determine phenotype and prevalence of antibiotic resistance; define genetic basis of tetracycline resistance in the LAB isolates and to investigate possibility for in vitro transmission of the tet genes. Total of 48 samples traditional Serbian cheeses (13 samples of Homolj cheese, 20 samples of Zlatar cheese and 15 samples of Sjenica cheese) were analyzed. All samples were plated on MRS-S agar plates in order to determine LAB count and on MRS-S agar plates with added tetracycline in doubling concentrations (1-256 μg/ml), in order to determine tetracycline resistant LAB subpopulation count. Average count of LAB in Homolj cheese was 7,65±1,09 log CFU/g, in Zlatar cheese 7,74±0,6 log CFU/g and in Sjenica cheese 7,47±0,71 log CFU/g. Addition of tetracycline in concentration of 64 μg/ml has considerably reduced LAB count, which was in Homolj cheese 3,98±2,28 log CFU/g, in Zlatar cheese 3,35±2,02 log CFU/g and in Sjenica cheese 3,93±2,24 log CFU/g...
AB  - Lanac hrane predstavlja jedan od mogućih puteva transmisije bakterija rezistentnih na antibiotike između populacija životinja i ljudi. Komensalne bakterije, poreklom od životinja, predstavljaju deo mikrobiote hrane životinjskog porekla, i često se prepoznaju kao „rezervoari“ gena za rezistenciju na antibiotike. Rezistencija na tetraciklin kod komensalnih bakterija je dobro dokumentovana i s obzirom da je prenosive prirode, koristi se kao model za praćenje ekologije rezistencije na antibiotike. Homoljski, zlatarski i sjenički sir su na nacionalnom nivou zaštićeni oznakom geografskog porekla. Proizvode se na tradicionalan način, od sirovog mleka, te bakterije mlečne kiseline, poreklom iz sirovine i/ili procesnog okruženja čine značajan deo mikrobiote tradicionalnih sireva Srbije. U naučnoj literaturi postoje brojni podaci o tome da bakterije mlečne kiseline mogu biti nosioci gena za rezistenciju na antibiotike. Ciljevi ove doktorske disertacije bili su: karakterizacija biodiverziteta tradicionalnih sireva Srbije, utvrđivanje fenotipa i prevalencije rezistencije na antibiotike i genetske baze rezistencije na tetraciklin kod izolata bakterija mlečne kiseline poreklom iz tradicionalnih sireva, kao i mogućnosti prenosa tet gena u uslovima in vitro. Ukupno 48 uzoraka tradicionalnih sireva (13 uzoraka homoljskog sira, 20 zlatarskog i 15 uzoraka sjeničkog sira), zasejano je na MRS-S agar radi utvrđivanja broja BMK i na MRS-S agar sa dodatkom tetraciklina u dvostrukorastućim koncentracijama (1-256 μg/ml), kako bi se utvrdila zastupljenost populacije BMK rezistentne na tetraciklin. Prosečan broj BMK u uzorcima homoljskog sira iznosio je 7,65±1,09 log CFU/g, u uzorcima zlatarskog sira 7,74±0,6 log CFU/g i u uzorcima sjeničkog sira 7,47±0,71 log CFU/g. Pri koncentraciji tetraciklina od 64 μg/ml primećeno je veće smanjenje broja BMK...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Tetracycline resistance in lactic acid bacteria isolated from traditional serbian cheeses
T1  - Rezistencija na tetraciklin bakterija mlečne kiseline izolovanih iz tradicionalnih sireva Srbije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10538
ER  - 

@phdthesis{
author = "Ledina, Tijana",
year = "2018",
abstract = "Food chain may represent a possible route for the transmission of antibiotic resistant bacteria between human and animal populations. Commensal bacteria originating from food-producing animals can contaminate food and by that means become a part of the complex food microbiota. Commensal bacteria can also serve as reservoirs for the antibiotic resistance genes. Tetracycline resistance in commensal bacteria is well documented and is coded with transmissible genes. Therefore, it is recognized as a model for the monitoring of antibiotic resistance ecology. Homolj, Zlatar and Sjenica cheese have protected geographical indication on the national level. As Homolj, Zlatar and Sjenica cheese are raw milk cheeses produced in the traditional manner, LAB originating from the raw milk and the environment constitute an important part of Serbian traditional cheeses’ microbiota. There are many data in the scientific literature that LAB can carry transmissible antibiotic resistance genes. The aims of this doctoral dissertation were to: characterize biodiversity of traditional Serbian cheeses; determine phenotype and prevalence of antibiotic resistance; define genetic basis of tetracycline resistance in the LAB isolates and to investigate possibility for in vitro transmission of the tet genes. Total of 48 samples traditional Serbian cheeses (13 samples of Homolj cheese, 20 samples of Zlatar cheese and 15 samples of Sjenica cheese) were analyzed. All samples were plated on MRS-S agar plates in order to determine LAB count and on MRS-S agar plates with added tetracycline in doubling concentrations (1-256 μg/ml), in order to determine tetracycline resistant LAB subpopulation count. Average count of LAB in Homolj cheese was 7,65±1,09 log CFU/g, in Zlatar cheese 7,74±0,6 log CFU/g and in Sjenica cheese 7,47±0,71 log CFU/g. Addition of tetracycline in concentration of 64 μg/ml has considerably reduced LAB count, which was in Homolj cheese 3,98±2,28 log CFU/g, in Zlatar cheese 3,35±2,02 log CFU/g and in Sjenica cheese 3,93±2,24 log CFU/g..., Lanac hrane predstavlja jedan od mogućih puteva transmisije bakterija rezistentnih na antibiotike između populacija životinja i ljudi. Komensalne bakterije, poreklom od životinja, predstavljaju deo mikrobiote hrane životinjskog porekla, i često se prepoznaju kao „rezervoari“ gena za rezistenciju na antibiotike. Rezistencija na tetraciklin kod komensalnih bakterija je dobro dokumentovana i s obzirom da je prenosive prirode, koristi se kao model za praćenje ekologije rezistencije na antibiotike. Homoljski, zlatarski i sjenički sir su na nacionalnom nivou zaštićeni oznakom geografskog porekla. Proizvode se na tradicionalan način, od sirovog mleka, te bakterije mlečne kiseline, poreklom iz sirovine i/ili procesnog okruženja čine značajan deo mikrobiote tradicionalnih sireva Srbije. U naučnoj literaturi postoje brojni podaci o tome da bakterije mlečne kiseline mogu biti nosioci gena za rezistenciju na antibiotike. Ciljevi ove doktorske disertacije bili su: karakterizacija biodiverziteta tradicionalnih sireva Srbije, utvrđivanje fenotipa i prevalencije rezistencije na antibiotike i genetske baze rezistencije na tetraciklin kod izolata bakterija mlečne kiseline poreklom iz tradicionalnih sireva, kao i mogućnosti prenosa tet gena u uslovima in vitro. Ukupno 48 uzoraka tradicionalnih sireva (13 uzoraka homoljskog sira, 20 zlatarskog i 15 uzoraka sjeničkog sira), zasejano je na MRS-S agar radi utvrđivanja broja BMK i na MRS-S agar sa dodatkom tetraciklina u dvostrukorastućim koncentracijama (1-256 μg/ml), kako bi se utvrdila zastupljenost populacije BMK rezistentne na tetraciklin. Prosečan broj BMK u uzorcima homoljskog sira iznosio je 7,65±1,09 log CFU/g, u uzorcima zlatarskog sira 7,74±0,6 log CFU/g i u uzorcima sjeničkog sira 7,47±0,71 log CFU/g. Pri koncentraciji tetraciklina od 64 μg/ml primećeno je veće smanjenje broja BMK...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Tetracycline resistance in lactic acid bacteria isolated from traditional serbian cheeses, Rezistencija na tetraciklin bakterija mlečne kiseline izolovanih iz tradicionalnih sireva Srbije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10538"
}

Ledina, T.. (2018). Tetracycline resistance in lactic acid bacteria isolated from traditional serbian cheeses. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_10538

Ledina T. Tetracycline resistance in lactic acid bacteria isolated from traditional serbian cheeses. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10538 .

Ledina, Tijana, "Tetracycline resistance in lactic acid bacteria isolated from traditional serbian cheeses" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10538 .

TY  - JOUR
AU  - Bjelica, Jelena
AU  - Jovanović, Ivan B.
AU  - Ilić, Vesna
AU  - Valčić, Olivera
AU  - Kirovski, Danijela
AU  - Gvozdić, Dragan
AU  - Milanović, Svetlana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1535
AB  - Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats
VL  - 67
IS  - 3
SP  - 340
EP  - 355
DO  - 10.1515/acve-2017-0028
ER  - 

@article{
author = "Bjelica, Jelena and Jovanović, Ivan B. and Ilić, Vesna and Valčić, Olivera and Kirovski, Danijela and Gvozdić, Dragan and Milanović, Svetlana",
year = "2017",
abstract = "Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats",
volume = "67",
number = "3",
pages = "340-355",
doi = "10.1515/acve-2017-0028"
}

Bjelica, J., Jovanović, I. B., Ilić, V., Valčić, O., Kirovski, D., Gvozdić, D.,& Milanović, S.. (2017). Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 340-355.
https://doi.org/10.1515/acve-2017-0028

Bjelica J, Jovanović IB, Ilić V, Valčić O, Kirovski D, Gvozdić D, Milanović S. Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd. 2017;67(3):340-355.
doi:10.1515/acve-2017-0028 .

Bjelica, Jelena, Jovanović, Ivan B., Ilić, Vesna, Valčić, Olivera, Kirovski, Danijela, Gvozdić, Dragan, Milanović, Svetlana, "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats" in Acta Veterinaria-Beograd, 67, no. 3 (2017):340-355,
https://doi.org/10.1515/acve-2017-0028 . .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Ilić, Vesna
AU  - Vasić, A.
AU  - Maletić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Savić, S.
AU  - Štukelj, Marina
AU  - Zdravković, Nemanja
AU  - Tzika, E. D.
AU  - Niculae, Mihaela
AU  - Milanov, Dubravka
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1525
AB  - The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms
VL  - 68
IS  - 3
SP  - 347
EP  - 354
DO  - 10.12681/jhvms.15488
ER  - 

@article{
author = "Bojkovski, Jovan and Ilić, Vesna and Vasić, A. and Maletić, Jelena and Vakanjac, Slobodanka and Savić, S. and Štukelj, Marina and Zdravković, Nemanja and Tzika, E. D. and Niculae, Mihaela and Milanov, Dubravka",
year = "2017",
abstract = "The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms",
volume = "68",
number = "3",
pages = "347-354",
doi = "10.12681/jhvms.15488"
}

Bojkovski, J., Ilić, V., Vasić, A., Maletić, J., Vakanjac, S., Savić, S., Štukelj, M., Zdravković, N., Tzika, E. D., Niculae, M.,& Milanov, D.. (2017). Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 68(3), 347-354.
https://doi.org/10.12681/jhvms.15488

Bojkovski J, Ilić V, Vasić A, Maletić J, Vakanjac S, Savić S, Štukelj M, Zdravković N, Tzika ED, Niculae M, Milanov D. Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society. 2017;68(3):347-354.
doi:10.12681/jhvms.15488 .

Bojkovski, Jovan, Ilić, Vesna, Vasić, A., Maletić, Jelena, Vakanjac, Slobodanka, Savić, S., Štukelj, Marina, Zdravković, Nemanja, Tzika, E. D., Niculae, Mihaela, Milanov, Dubravka, "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms" in Journal of the Hellenic Veterinary Medical Society, 68, no. 3 (2017):347-354,
https://doi.org/10.12681/jhvms.15488 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovacić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valčić, Olivera
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1519
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
VL  - 67
IS  - 4
SP  - 508
EP  - 524
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovacić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valčić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
volume = "67",
number = "4",
pages = "508-524",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovacić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valčić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovacić M, Ilić V, Gvozdić D, Savić O, Doković R, Valčić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovacić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valčić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Bulajić, Snežana
AU  - Čolović, Svetlana
AU  - Mišić, Dušan
AU  - Đorđević, Jasna
AU  - Savić Radovanović, Radoslava
AU  - Ašanin, Jelena
AU  - Ledina, Tijana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1542
AB  - This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
T1  - Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia
VL  - 52
IS  - 12
SP  - 864
EP  - 870
DO  - 10.1080/03601234.2017.1361764
ER  - 

@article{
author = "Bulajić, Snežana and Čolović, Svetlana and Mišić, Dušan and Đorđević, Jasna and Savić Radovanović, Radoslava and Ašanin, Jelena and Ledina, Tijana",
year = "2017",
abstract = "This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural",
title = "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia",
volume = "52",
number = "12",
pages = "864-870",
doi = "10.1080/03601234.2017.1361764"
}

Bulajić, S., Čolović, S., Mišić, D., Đorđević, J., Savić Radovanović, R., Ašanin, J.,& Ledina, T.. (2017). Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
Taylor & Francis Inc, Philadelphia., 52(12), 864-870.
https://doi.org/10.1080/03601234.2017.1361764

Bulajić S, Čolović S, Mišić D, Đorđević J, Savić Radovanović R, Ašanin J, Ledina T. Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural. 2017;52(12):864-870.
doi:10.1080/03601234.2017.1361764 .

Bulajić, Snežana, Čolović, Svetlana, Mišić, Dušan, Đorđević, Jasna, Savić Radovanović, Radoslava, Ašanin, Jelena, Ledina, Tijana, "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia" in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 52, no. 12 (2017):864-870,
https://doi.org/10.1080/03601234.2017.1361764 . .

TY  - JOUR
AU  - Bulajić, Snežana
AU  - Mijačević, Zora
AU  - Ledina, Tijana
AU  - Golić, Bojan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1296
AB  - Sjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheeps milk in the southern part of Serbia - Sjenica Pester plateau. The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated. During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 10(5) cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identified as Staphylococcus intermedius (11 isolates) and Staphylococcus aureus (1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profile in coagulase positive staphylococci. Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profile in enterococci. All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins, tet(M) and tet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showed tet(M) gene presence, while 2 lactobacilli isolates revealed the presence of tet(K) gene.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Safety evaluation of Sjenica cheese with regard to coagulase-positive staphylococci and antibiotic resistance of lactic acid bacteria and staphylococci
VL  - 65
IS  - 4
SP  - 518
EP  - 537
DO  - 10.1515/acve-2015-0044
ER  - 

@article{
author = "Bulajić, Snežana and Mijačević, Zora and Ledina, Tijana and Golić, Bojan",
year = "2015",
abstract = "Sjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheeps milk in the southern part of Serbia - Sjenica Pester plateau. The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated. During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 10(5) cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identified as Staphylococcus intermedius (11 isolates) and Staphylococcus aureus (1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profile in coagulase positive staphylococci. Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profile in enterococci. All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins, tet(M) and tet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showed tet(M) gene presence, while 2 lactobacilli isolates revealed the presence of tet(K) gene.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Safety evaluation of Sjenica cheese with regard to coagulase-positive staphylococci and antibiotic resistance of lactic acid bacteria and staphylococci",
volume = "65",
number = "4",
pages = "518-537",
doi = "10.1515/acve-2015-0044"
}

Bulajić, S., Mijačević, Z., Ledina, T.,& Golić, B.. (2015). Safety evaluation of Sjenica cheese with regard to coagulase-positive staphylococci and antibiotic resistance of lactic acid bacteria and staphylococci. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 518-537.
https://doi.org/10.1515/acve-2015-0044

Bulajić S, Mijačević Z, Ledina T, Golić B. Safety evaluation of Sjenica cheese with regard to coagulase-positive staphylococci and antibiotic resistance of lactic acid bacteria and staphylococci. in Acta Veterinaria-Beograd. 2015;65(4):518-537.
doi:10.1515/acve-2015-0044 .

Bulajić, Snežana, Mijačević, Zora, Ledina, Tijana, Golić, Bojan, "Safety evaluation of Sjenica cheese with regard to coagulase-positive staphylococci and antibiotic resistance of lactic acid bacteria and staphylococci" in Acta Veterinaria-Beograd, 65, no. 4 (2015):518-537,
https://doi.org/10.1515/acve-2015-0044 . .

TY  - CONF
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2932
PB  - Novi Sad : Institute of Food Technology
C3  - II International Congress – Food Technology, Quality and Safety, Novi Sad, October 28-30, 2014
T1  - Antibiotic resistance of autochthonous potential probiotic bacteria
SP  - 500
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2932
ER  - 

@conference{
year = "2014",
publisher = "Novi Sad : Institute of Food Technology",
journal = "II International Congress – Food Technology, Quality and Safety, Novi Sad, October 28-30, 2014",
title = "Antibiotic resistance of autochthonous potential probiotic bacteria",
pages = "500-504",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2932"
}

(2014). Antibiotic resistance of autochthonous potential probiotic bacteria. in II International Congress – Food Technology, Quality and Safety, Novi Sad, October 28-30, 2014
Novi Sad : Institute of Food Technology., 500-504.
https://hdl.handle.net/21.15107/rcub_veterinar_2932

Antibiotic resistance of autochthonous potential probiotic bacteria. in II International Congress – Food Technology, Quality and Safety, Novi Sad, October 28-30, 2014. 2014;:500-504.
https://hdl.handle.net/21.15107/rcub_veterinar_2932 .

"Antibiotic resistance of autochthonous potential probiotic bacteria" in II International Congress – Food Technology, Quality and Safety, Novi Sad, October 28-30, 2014 (2014):500-504,
https://hdl.handle.net/21.15107/rcub_veterinar_2932 .

TY  - CHAP
AU  - Radulović, Zorica
AU  - Petrović, Tanja
AU  - Bulajić, Snežana
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2905
AB  - Lactic acid bacteria (LAB) are a heterogeneous group of bacteria widely distributed in
nature. These bacteria are found in gastrointestinal (GI) and urogenital tract of humans and
animals; they are present on plant material, in milk and meat, and numerous fermented
foods. Lactic acid bacteria have been associated with traditional dairy products, cereals,
vegetable and meat fermented foods, due to their natural presence leading to spontaneous
fermentation. They are also used as starter cultures in industrial food production, as well as
in the production of probiotic products due to their potential health benefits to consumer.
Milk and dairy products are the most examined food system for the delivery of probiotic
bacteria to the human gut. The probiotic concept has progressed and is now in the focus of
different research. Significant improvements have been made in selection and
characterization of new cultures and their application in food production.
The food products, which are produced by traditional methods, exhibit a rich biodiversity
with the respect to bacterial contents. From these products, new probiotic strains with the
potential functional properties can been isolated and selected. The selected strains have to
be further characterized in order to be used in the food industry. Before the probiotics can
benefit human health, they must fulfill several criteria including: a) scientifically validated
health properties; b) good technological properties meaning that they can be manufactured
and incorporated into food products without loosing viability, functionality and technological
performance; c) high survival through the upper gastrointestinal tract and high viability at
its site of action; d) antagonistic activity to pathogens; e) antibiotic susceptibility; and f) to be
able to function in the gut environment. Bearing in mind importance of antibiotic resistance
of LAB in food chain, antibiotic susceptibility of potential probiotic strains is a very
important criteria for their selection.
In the recent decade, releasing of antibiotics in biosphere seriously increased, leading to a
strong selective pressure for the emergence and persistence of resistant LAB strains. Since
LAB are naturally present in traditionally made fermented food and GI tract and are also
added as starter culture or probiotic bacteria in industrial food production, concerns have
been raised about the antibiotic resistance of these beneficial bacteria strains. Probiotic
bacteria can help maintaining balance in gastrointestinal tract in cases of diarrhea caused by
antibiotic treatment. However, there is high risk associated with the ability of these resistant strains to transmit the resistance gene to pathogenic bacteria in gut microbiota. This can
complicate the treatment of a patient with an antibiotic resistant bacterial infection or
disease. The circulation of genes coding for antibiotic resistance from beneficial LAB in the
food chain via animals to humans is a complex problem. Therefore, there is a need to evaluate
the safety of potential probitic strains regarding their ability to acquire and disseminate
antibiotic resistance determinants in selection of LAB.
In this study, importance of LAB in the food chain will be reviewed. Morphological and
biochemical characteristics of lactobacilli, bifidobactera and enterococci, as well as criteria
for probiotic selection and role of probiotics in health benefit will be discussed. Antibiotic
susceptibility as criteria for potential probiotic bacteria selection and mechanisms of gene
transfers will be considered.
PB  - InTech
T2  - Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium
T1  - Antibiotic Susceptibility of Probiotic Bacteria
SP  - 549
EP  - 576
DO  - 10.5772/28915
ER  - 

@inbook{
author = "Radulović, Zorica and Petrović, Tanja and Bulajić, Snežana",
year = "2012",
abstract = "Lactic acid bacteria (LAB) are a heterogeneous group of bacteria widely distributed in
nature. These bacteria are found in gastrointestinal (GI) and urogenital tract of humans and
animals; they are present on plant material, in milk and meat, and numerous fermented
foods. Lactic acid bacteria have been associated with traditional dairy products, cereals,
vegetable and meat fermented foods, due to their natural presence leading to spontaneous
fermentation. They are also used as starter cultures in industrial food production, as well as
in the production of probiotic products due to their potential health benefits to consumer.
Milk and dairy products are the most examined food system for the delivery of probiotic
bacteria to the human gut. The probiotic concept has progressed and is now in the focus of
different research. Significant improvements have been made in selection and
characterization of new cultures and their application in food production.
The food products, which are produced by traditional methods, exhibit a rich biodiversity
with the respect to bacterial contents. From these products, new probiotic strains with the
potential functional properties can been isolated and selected. The selected strains have to
be further characterized in order to be used in the food industry. Before the probiotics can
benefit human health, they must fulfill several criteria including: a) scientifically validated
health properties; b) good technological properties meaning that they can be manufactured
and incorporated into food products without loosing viability, functionality and technological
performance; c) high survival through the upper gastrointestinal tract and high viability at
its site of action; d) antagonistic activity to pathogens; e) antibiotic susceptibility; and f) to be
able to function in the gut environment. Bearing in mind importance of antibiotic resistance
of LAB in food chain, antibiotic susceptibility of potential probiotic strains is a very
important criteria for their selection.
In the recent decade, releasing of antibiotics in biosphere seriously increased, leading to a
strong selective pressure for the emergence and persistence of resistant LAB strains. Since
LAB are naturally present in traditionally made fermented food and GI tract and are also
added as starter culture or probiotic bacteria in industrial food production, concerns have
been raised about the antibiotic resistance of these beneficial bacteria strains. Probiotic
bacteria can help maintaining balance in gastrointestinal tract in cases of diarrhea caused by
antibiotic treatment. However, there is high risk associated with the ability of these resistant strains to transmit the resistance gene to pathogenic bacteria in gut microbiota. This can
complicate the treatment of a patient with an antibiotic resistant bacterial infection or
disease. The circulation of genes coding for antibiotic resistance from beneficial LAB in the
food chain via animals to humans is a complex problem. Therefore, there is a need to evaluate
the safety of potential probitic strains regarding their ability to acquire and disseminate
antibiotic resistance determinants in selection of LAB.
In this study, importance of LAB in the food chain will be reviewed. Morphological and
biochemical characteristics of lactobacilli, bifidobactera and enterococci, as well as criteria
for probiotic selection and role of probiotics in health benefit will be discussed. Antibiotic
susceptibility as criteria for potential probiotic bacteria selection and mechanisms of gene
transfers will be considered.",
publisher = "InTech",
journal = "Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium",
booktitle = "Antibiotic Susceptibility of Probiotic Bacteria",
pages = "549-576",
doi = "10.5772/28915"
}

Radulović, Z., Petrović, T.,& Bulajić, S.. (2012). Antibiotic Susceptibility of Probiotic Bacteria. in Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium
InTech., 549-576.
https://doi.org/10.5772/28915

Radulović Z, Petrović T, Bulajić S. Antibiotic Susceptibility of Probiotic Bacteria. in Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium. 2012;:549-576.
doi:10.5772/28915 .

Radulović, Zorica, Petrović, Tanja, Bulajić, Snežana, "Antibiotic Susceptibility of Probiotic Bacteria" in Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium (2012):549-576,
https://doi.org/10.5772/28915 . .