TY  - CONF
AU  - Stajković, Silvana
AU  - Vasilev, Dragan
AU  - Dimitrijević, Mirjana
AU  - Karabasil, Nedjeljko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2302
AB  - Knowledge of the measurement uncertainty of test results is fundamentally important for laboratories, their customers and all parties using and interpreting these results. In conformity assessment, a measurement result is used to decide if an item of interest conforms to a specified requirement. Because of measurement uncertainty, there is always the risk of incorrectly deciding whether or not an item conforms to a specified requirement based on the measured value of a property of the item. Conformity assessment can be quite challenging when the entity measured is so close to the tolerance limits of the specification that its uncertainty, however estimated, critically affects decision-making. In such cases, different decision rules can be used to make statements of conformity. The aim of this paper is to provide a survey of methods for the evaluation of measurement uncertainty in testing, as well as to stress the need for appropriate estimation of measurement uncertainty. This paper also aims to assist testing laboratories in understanding the different decision rules used in conformity assessment and level of risk (such as false accept and false reject) associated with the decision rule employed.
PB  - IOP Publishing Ltd
C3  - IOP Conference Series: Earth and Environmental Science
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Uncertainty of measurement and conformity assessment
VL  - 854
IS  - 1
SP  - 012093
DO  - 10.1088/1755-1315/854/1/012093
ER  - 

@conference{
author = "Stajković, Silvana and Vasilev, Dragan and Dimitrijević, Mirjana and Karabasil, Nedjeljko",
year = "2021",
abstract = "Knowledge of the measurement uncertainty of test results is fundamentally important for laboratories, their customers and all parties using and interpreting these results. In conformity assessment, a measurement result is used to decide if an item of interest conforms to a specified requirement. Because of measurement uncertainty, there is always the risk of incorrectly deciding whether or not an item conforms to a specified requirement based on the measured value of a property of the item. Conformity assessment can be quite challenging when the entity measured is so close to the tolerance limits of the specification that its uncertainty, however estimated, critically affects decision-making. In such cases, different decision rules can be used to make statements of conformity. The aim of this paper is to provide a survey of methods for the evaluation of measurement uncertainty in testing, as well as to stress the need for appropriate estimation of measurement uncertainty. This paper also aims to assist testing laboratories in understanding the different decision rules used in conformity assessment and level of risk (such as false accept and false reject) associated with the decision rule employed.",
publisher = "IOP Publishing Ltd",
journal = "IOP Conference Series: Earth and Environmental Science, IOP Conference Series: Earth and Environmental Science",
title = "Uncertainty of measurement and conformity assessment",
volume = "854",
number = "1",
pages = "012093",
doi = "10.1088/1755-1315/854/1/012093"
}

Stajković, S., Vasilev, D., Dimitrijević, M.,& Karabasil, N.. (2021). Uncertainty of measurement and conformity assessment. in IOP Conference Series: Earth and Environmental Science
IOP Publishing Ltd., 854(1), 012093.
https://doi.org/10.1088/1755-1315/854/1/012093

Stajković S, Vasilev D, Dimitrijević M, Karabasil N. Uncertainty of measurement and conformity assessment. in IOP Conference Series: Earth and Environmental Science. 2021;854(1):012093.
doi:10.1088/1755-1315/854/1/012093 .

Stajković, Silvana, Vasilev, Dragan, Dimitrijević, Mirjana, Karabasil, Nedjeljko, "Uncertainty of measurement and conformity assessment" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021):012093,
https://doi.org/10.1088/1755-1315/854/1/012093 . .

TY  - JOUR
AU  - Tamara, Ilić
AU  - Nataša, Mihajlović
AU  - Sanda, Dimitrijević
AU  - Danica, Bogunović
AU  - Nenadović, Katarina
AU  - Bojan, Gajić
AU  - Tamaš, Petrović
AU  - Darko, Despotović
AU  - Becskei, Zsolt
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2223
AB  - Breeding of wild boars is a significant part of the hunting economy; however, hogs are associated with zoonotic infection. This study assessed the prevalence and degree of parasitic infections that exist in wild boars from two hunting grounds in the Autonomous Province of Vojvodina, Republic of Serbia.
PB  - Springer
T2  - Acta Parasitologica
T1  - The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method
VL  - 66
IS  - 1
SP  - 104
EP  - 115
DO  - 10.1007/s11686-020-00261-8
ER  - 

@article{
author = "Tamara, Ilić and Nataša, Mihajlović and Sanda, Dimitrijević and Danica, Bogunović and Nenadović, Katarina and Bojan, Gajić and Tamaš, Petrović and Darko, Despotović and Becskei, Zsolt",
year = "2021",
abstract = "Breeding of wild boars is a significant part of the hunting economy; however, hogs are associated with zoonotic infection. This study assessed the prevalence and degree of parasitic infections that exist in wild boars from two hunting grounds in the Autonomous Province of Vojvodina, Republic of Serbia.",
publisher = "Springer",
journal = "Acta Parasitologica",
title = "The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method",
volume = "66",
number = "1",
pages = "104-115",
doi = "10.1007/s11686-020-00261-8"
}

Tamara, I., Nataša, M., Sanda, D., Danica, B., Nenadović, K., Bojan, G., Tamaš, P., Darko, D.,& Becskei, Z.. (2021). The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method. in Acta Parasitologica
Springer., 66(1), 104-115.
https://doi.org/10.1007/s11686-020-00261-8

Tamara I, Nataša M, Sanda D, Danica B, Nenadović K, Bojan G, Tamaš P, Darko D, Becskei Z. The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method. in Acta Parasitologica. 2021;66(1):104-115.
doi:10.1007/s11686-020-00261-8 .

Tamara, Ilić, Nataša, Mihajlović, Sanda, Dimitrijević, Danica, Bogunović, Nenadović, Katarina, Bojan, Gajić, Tamaš, Petrović, Darko, Despotović, Becskei, Zsolt, "The Prevalence and Degree of Endoparasitic Infections in Wild Boars Using the Semi-quantitative Fecal Egg Count Method" in Acta Parasitologica, 66, no. 1 (2021):104-115,
https://doi.org/10.1007/s11686-020-00261-8 . .

TY  - JOUR
AU  - Aleksić, Jelena
AU  - Stepanović, Predrag
AU  - Dimitrijević, Sanda
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Davidov, Ivana
AU  - Aleksić-Agelidis, Aleksandra
AU  - Ilić, Tamara
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1971
AB  - Purpose: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. Methods: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. Results: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. Discussion: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. Conclusion: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Acta Parasitologica
T1  - Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores
VL  - 65
IS  - 4
SP  - 954
EP  - 962
DO  - 10.1007/s11686-020-00244-9
ER  - 

@article{
author = "Aleksić, Jelena and Stepanović, Predrag and Dimitrijević, Sanda and Gajić, Bojan and Bogunović, Danica and Davidov, Ivana and Aleksić-Agelidis, Aleksandra and Ilić, Tamara",
year = "2020",
abstract = "Purpose: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. Methods: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. Results: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. Discussion: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. Conclusion: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Acta Parasitologica",
title = "Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores",
volume = "65",
number = "4",
pages = "954-962",
doi = "10.1007/s11686-020-00244-9"
}

Aleksić, J., Stepanović, P., Dimitrijević, S., Gajić, B., Bogunović, D., Davidov, I., Aleksić-Agelidis, A.,& Ilić, T.. (2020). Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores. in Acta Parasitologica
Springer Science and Business Media Deutschland GmbH., 65(4), 954-962.
https://doi.org/10.1007/s11686-020-00244-9

Aleksić J, Stepanović P, Dimitrijević S, Gajić B, Bogunović D, Davidov I, Aleksić-Agelidis A, Ilić T. Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores. in Acta Parasitologica. 2020;65(4):954-962.
doi:10.1007/s11686-020-00244-9 .

Aleksić, Jelena, Stepanović, Predrag, Dimitrijević, Sanda, Gajić, Bojan, Bogunović, Danica, Davidov, Ivana, Aleksić-Agelidis, Aleksandra, Ilić, Tamara, "Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores" in Acta Parasitologica, 65, no. 4 (2020):954-962,
https://doi.org/10.1007/s11686-020-00244-9 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Savić, Božidar
AU  - Radojičić, Sonja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1793
AB  - Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The performance of seven molecular methods for the detection of prrsv
VL  - 70
IS  - 1
SP  - 51
EP  - 57
DO  - 10.2478/acve-2020-0003
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Veljović, Ljubiša and Valčić, Miroslav and Stević, Nataša and Savić, Božidar and Radojičić, Sonja",
year = "2020",
abstract = "Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The performance of seven molecular methods for the detection of prrsv",
volume = "70",
number = "1",
pages = "51-57",
doi = "10.2478/acve-2020-0003"
}

Milićević, V., Kureljušić, B., Veljović, L., Valčić, M., Stević, N., Savić, B.,& Radojičić, S.. (2020). The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 51-57.
https://doi.org/10.2478/acve-2020-0003

Milićević V, Kureljušić B, Veljović L, Valčić M, Stević N, Savić B, Radojičić S. The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd. 2020;70(1):51-57.
doi:10.2478/acve-2020-0003 .

Milićević, Vesna, Kureljušić, Branislav, Veljović, Ljubiša, Valčić, Miroslav, Stević, Nataša, Savić, Božidar, Radojičić, Sonja, "The performance of seven molecular methods for the detection of prrsv" in Acta Veterinaria-Beograd, 70, no. 1 (2020):51-57,
https://doi.org/10.2478/acve-2020-0003 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Hoffmann, Bernd
AU  - Dietze, Klaas
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1795
AB  - Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.
PB  - BMC, London
T2  - Virology Journal
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA
VL  - 17
IS  - 1
SP  - 28
DO  - 10.1186/s12985-020-01298-x
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Hoffmann, Bernd and Dietze, Klaas",
year = "2020",
abstract = "Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.",
publisher = "BMC, London",
journal = "Virology Journal",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA",
volume = "17",
number = "1",
pages = "28",
doi = "10.1186/s12985-020-01298-x"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Hoffmann, B.,& Dietze, K.. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal
BMC, London., 17(1), 28.
https://doi.org/10.1186/s12985-020-01298-x

Milovanović M, Milićević V, Radojičić S, Valčić M, Hoffmann B, Dietze K. Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal. 2020;17(1):28.
doi:10.1186/s12985-020-01298-x .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Hoffmann, Bernd, Dietze, Klaas, "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA" in Virology Journal, 17, no. 1 (2020):28,
https://doi.org/10.1186/s12985-020-01298-x . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Radojičić, Sonja
AU  - Debeljak, Zoran
AU  - Šekler, Milanko
AU  - Ćirić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797
AB  - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
PB  - Elsevier, Amsterdam
T2  - Saudi Journal of Biological Sciences
T1  - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
VL  - 27
IS  - 1
SP  - 518
EP  - 523
DO  - 10.1016/j.sjbs.2019.11.012
ER  - 

@article{
author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena",
year = "2020",
abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Journal of Biological Sciences",
title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia",
volume = "27",
number = "1",
pages = "518-523",
doi = "10.1016/j.sjbs.2019.11.012"
}

Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences
Elsevier, Amsterdam., 27(1), 518-523.
https://doi.org/10.1016/j.sjbs.2019.11.012

Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523.
doi:10.1016/j.sjbs.2019.11.012 .

Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523,
https://doi.org/10.1016/j.sjbs.2019.11.012 . .

TY  - THES
AU  - Bogunović, Danica
PY  - 2019
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51495439
UR  - http://nardus.mpn.gov.rs/123456789/11278
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/53
AB  - In this doctoral dissertation, the presence of the causative agent of Q fever - the bacterium Coxiella burnetii (C. burnetii) in stray dogs has been examined using molecular and serological methods. Molecular method was used for the detection of the agent in the reproductive tissues of stray dogs, as well as in the ticks recovered from the examined animals, while immunoenzyme test was used for the detection of specific serological response in the examined animals. A polymerase chain reaction (Trans-PCR) targeting IS1111 element of C. burnetii was used for the detection of C. burnetii DNA in the samples, while a modified commercial immunoassay (ELISA) was used for the detection of specific antibodies against C. burnetii in the sera of the examined dogs. In this study, 316 ticks were recovered from 51 stray dogs and three tick species were identified: Rhipicephalus sanguineus, Ixodes ricinus and Dermacentor reticulatus. The presence of C. burnetii DNA was established in 10.53% (228/24) R. sanguineus ticks, which originated from seven dogs. Reproductive tissues of dogs were collected from a total of 105 dogs - 74 females (70.48%) and 31 males (29.52%). The presence of C. burentii DNA has been detected in 20.95% (22/105) dogs. C. burnetii DNA was detected in 7 16.13% (5/31) samples of dog testicles and in 22.97% (17/74) samples of uteri and ovaries...
AB  - U ovoj doktorskoj disertaciji ispitano je prisustvo uzročnika kju groznice - bakterije Coxiella burnetii (C. burnetii) kod nevlasničkih pasa poreklom sa teritorije grada Beograda, primenom molekularnih i seroloških metoda. Molekularni metod je korišćen za otkrivanje uzročnika u reproduktivnim tkivima nevlasničkih pasa, kao i u krpeljima prisutnim na ispitivanim životinjama, dok je imunoenzimskim testom utvrđeno prisustvo specifičnog serološkog odgovora kod ispitivanih životinja. Za otkrivanje prisustva DNK C. burnetii u uzrocima korišćena je lančana reakcija polimeraze (Trans-PCR) kojom se umnožava IS1111 fragment C. burnetii, dok je za otkrivanje specifičnih antitela protiv C. burnetii u serumima ispitivanih pasa korišćen modifikovani komercijalni imunoenzimski test (ELISA). U istraživanju je sakupljeno 316 krpelja sa 51 nevlasničkog psa i identifikovane su tri vrste krpelja: Rhipicephalus sanguineus, Ixodes ricinus i Dermacentor reticulatus. Prisustvo DNK C. burnetii ustanovljeno je u 10,53% (24/228) krpelja vrste R. sanguineus, koji su uzorkovani sa sedam pasa. Reproduktivna tkiva pasa sakupljena su od ukupno 105 pasa - 74 ženke (70,48%) i 31 mužjaka (29,52%). DNK C. burentii je ustanovljena kod 20,95% (22/105) pasa i to u 16,13% (5/31) uzoraka semenika pasa, odnosno u 22,97% (17/74) uzoraka materica i jajnika. Kod 29,52% (31/105) pasa je 5 ustanovljeno prisustvo specifičnih antitela protiv C. burnetii i to 32,26% (10/31) mužjaka i 28,38% (21/74) ispitanih ženki...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Molecular and serological examination of the presence of the bacterium Coxiella burnetii in the tissues of dogs and ticks (Acari: Ixodidae) recovered from the examined animals
T1  - Molekularna i serološka istraživanja prisustva bakterije Coxiella burnetii u tkivima pasa i krpeljima (Acari: Ixodidae) sakupljenim sa ispitivanih životinja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11278
ER  - 

@phdthesis{
author = "Bogunović, Danica",
year = "2019",
abstract = "In this doctoral dissertation, the presence of the causative agent of Q fever - the bacterium Coxiella burnetii (C. burnetii) in stray dogs has been examined using molecular and serological methods. Molecular method was used for the detection of the agent in the reproductive tissues of stray dogs, as well as in the ticks recovered from the examined animals, while immunoenzyme test was used for the detection of specific serological response in the examined animals. A polymerase chain reaction (Trans-PCR) targeting IS1111 element of C. burnetii was used for the detection of C. burnetii DNA in the samples, while a modified commercial immunoassay (ELISA) was used for the detection of specific antibodies against C. burnetii in the sera of the examined dogs. In this study, 316 ticks were recovered from 51 stray dogs and three tick species were identified: Rhipicephalus sanguineus, Ixodes ricinus and Dermacentor reticulatus. The presence of C. burnetii DNA was established in 10.53% (228/24) R. sanguineus ticks, which originated from seven dogs. Reproductive tissues of dogs were collected from a total of 105 dogs - 74 females (70.48%) and 31 males (29.52%). The presence of C. burentii DNA has been detected in 20.95% (22/105) dogs. C. burnetii DNA was detected in 7 16.13% (5/31) samples of dog testicles and in 22.97% (17/74) samples of uteri and ovaries..., U ovoj doktorskoj disertaciji ispitano je prisustvo uzročnika kju groznice - bakterije Coxiella burnetii (C. burnetii) kod nevlasničkih pasa poreklom sa teritorije grada Beograda, primenom molekularnih i seroloških metoda. Molekularni metod je korišćen za otkrivanje uzročnika u reproduktivnim tkivima nevlasničkih pasa, kao i u krpeljima prisutnim na ispitivanim životinjama, dok je imunoenzimskim testom utvrđeno prisustvo specifičnog serološkog odgovora kod ispitivanih životinja. Za otkrivanje prisustva DNK C. burnetii u uzrocima korišćena je lančana reakcija polimeraze (Trans-PCR) kojom se umnožava IS1111 fragment C. burnetii, dok je za otkrivanje specifičnih antitela protiv C. burnetii u serumima ispitivanih pasa korišćen modifikovani komercijalni imunoenzimski test (ELISA). U istraživanju je sakupljeno 316 krpelja sa 51 nevlasničkog psa i identifikovane su tri vrste krpelja: Rhipicephalus sanguineus, Ixodes ricinus i Dermacentor reticulatus. Prisustvo DNK C. burnetii ustanovljeno je u 10,53% (24/228) krpelja vrste R. sanguineus, koji su uzorkovani sa sedam pasa. Reproduktivna tkiva pasa sakupljena su od ukupno 105 pasa - 74 ženke (70,48%) i 31 mužjaka (29,52%). DNK C. burentii je ustanovljena kod 20,95% (22/105) pasa i to u 16,13% (5/31) uzoraka semenika pasa, odnosno u 22,97% (17/74) uzoraka materica i jajnika. Kod 29,52% (31/105) pasa je 5 ustanovljeno prisustvo specifičnih antitela protiv C. burnetii i to 32,26% (10/31) mužjaka i 28,38% (21/74) ispitanih ženki...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Molecular and serological examination of the presence of the bacterium Coxiella burnetii in the tissues of dogs and ticks (Acari: Ixodidae) recovered from the examined animals, Molekularna i serološka istraživanja prisustva bakterije Coxiella burnetii u tkivima pasa i krpeljima (Acari: Ixodidae) sakupljenim sa ispitivanih životinja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11278"
}

Bogunović, D.. (2019). Molecular and serological examination of the presence of the bacterium Coxiella burnetii in the tissues of dogs and ticks (Acari: Ixodidae) recovered from the examined animals. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_11278

Bogunović D. Molecular and serological examination of the presence of the bacterium Coxiella burnetii in the tissues of dogs and ticks (Acari: Ixodidae) recovered from the examined animals. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11278 .

Bogunović, Danica, "Molecular and serological examination of the presence of the bacterium Coxiella burnetii in the tissues of dogs and ticks (Acari: Ixodidae) recovered from the examined animals" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11278 .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Dondović, Novica
AU  - Nenadović, Katarina
AU  - Bogunović, Danica
AU  - Aleksić, Jelena
AU  - Dimitrijević, Sanda
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1715
AB  - IntroductionTimely and valid diagnostic of parasitic diseases are prerequisites for profitable traditional poultry breeding, which enables adequate prophylaxis and effective therapy. This research is retrospective of the prevalence of endoparasites in 880 hens, 291 ducks, 171 geese and 302 turkeys in extensive poultry breeding in the Republic of Serbia.Materials and MethodsQualitative parasitological examination was done by conventional gravitational flotation method and sedimentation method. In rare cases of animal death, an autopsy was performed.ResultsIn the period from 2012 to 2017, in 12 examined localities in the Republic of Serbia, single or mixed infections with endoparasites Eimeria spp., Ascaridia galli, Heterakis gallinarum, Capillaria contorta, Amidostomum anseris and Syngamus trachea were diagnosed. The highest prevalence of ascaridiosis was detected in hens (15.69-24.05%), trichostrongylidosis in ducks (20.53-30.19%), heterakiosis (20.89- 25.86%) and capillariosis (20.68-26.08%) in geese, and syngamosis (23.39%) and capillariosis (14.28- 24.17%) in turkeys. Endoparasites were the most prevalent in hens in south Serbia (63.21%), in ducks in central Serbia (54.71%) and in geese (46.55%) and turkeys (58.24%) in north Serbia. Among mixed infections predominant was polyparasitism of Heterakis spp. and Capillaria spp.ConclusionsAn epizootiological and clinical diagnostic approach based on the results of parasitological screening is very important from the aspect of organic poultry farming.
PB  - Springer International Publishing Ag, Cham
T2  - Acta Parasitologica
T1  - Importance of Parasitological Screening in Extensive Poultry Farming Based on Organic Production
VL  - 64
IS  - 2
SP  - 336
EP  - 346
DO  - 10.2478/s11686-019-00042-y
ER  - 

@article{
author = "Ilić, Tamara and Dondović, Novica and Nenadović, Katarina and Bogunović, Danica and Aleksić, Jelena and Dimitrijević, Sanda",
year = "2019",
abstract = "IntroductionTimely and valid diagnostic of parasitic diseases are prerequisites for profitable traditional poultry breeding, which enables adequate prophylaxis and effective therapy. This research is retrospective of the prevalence of endoparasites in 880 hens, 291 ducks, 171 geese and 302 turkeys in extensive poultry breeding in the Republic of Serbia.Materials and MethodsQualitative parasitological examination was done by conventional gravitational flotation method and sedimentation method. In rare cases of animal death, an autopsy was performed.ResultsIn the period from 2012 to 2017, in 12 examined localities in the Republic of Serbia, single or mixed infections with endoparasites Eimeria spp., Ascaridia galli, Heterakis gallinarum, Capillaria contorta, Amidostomum anseris and Syngamus trachea were diagnosed. The highest prevalence of ascaridiosis was detected in hens (15.69-24.05%), trichostrongylidosis in ducks (20.53-30.19%), heterakiosis (20.89- 25.86%) and capillariosis (20.68-26.08%) in geese, and syngamosis (23.39%) and capillariosis (14.28- 24.17%) in turkeys. Endoparasites were the most prevalent in hens in south Serbia (63.21%), in ducks in central Serbia (54.71%) and in geese (46.55%) and turkeys (58.24%) in north Serbia. Among mixed infections predominant was polyparasitism of Heterakis spp. and Capillaria spp.ConclusionsAn epizootiological and clinical diagnostic approach based on the results of parasitological screening is very important from the aspect of organic poultry farming.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Acta Parasitologica",
title = "Importance of Parasitological Screening in Extensive Poultry Farming Based on Organic Production",
volume = "64",
number = "2",
pages = "336-346",
doi = "10.2478/s11686-019-00042-y"
}

Ilić, T., Dondović, N., Nenadović, K., Bogunović, D., Aleksić, J.,& Dimitrijević, S.. (2019). Importance of Parasitological Screening in Extensive Poultry Farming Based on Organic Production. in Acta Parasitologica
Springer International Publishing Ag, Cham., 64(2), 336-346.
https://doi.org/10.2478/s11686-019-00042-y

Ilić T, Dondović N, Nenadović K, Bogunović D, Aleksić J, Dimitrijević S. Importance of Parasitological Screening in Extensive Poultry Farming Based on Organic Production. in Acta Parasitologica. 2019;64(2):336-346.
doi:10.2478/s11686-019-00042-y .

Ilić, Tamara, Dondović, Novica, Nenadović, Katarina, Bogunović, Danica, Aleksić, Jelena, Dimitrijević, Sanda, "Importance of Parasitological Screening in Extensive Poultry Farming Based on Organic Production" in Acta Parasitologica, 64, no. 2 (2019):336-346,
https://doi.org/10.2478/s11686-019-00042-y . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Kulišić, Zoran
AU  - Despotović, Darko
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Dimitrijević, Sanda
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2178
AB  - Kontrola gastrointestinalnih parazita u različitim sistemima uzgoja preživara zasnivana je na vakcinaciji, hemoterapiji, poboljšanju kvaliteta menadžmenta stada i korišćenju genetskih potencijala domaćina. Širom sveta dominira strategija kontrole helminata zasnovana na čestoj upotrebi antihelmintika, koja se smatra neodrživom, s obzirom na pojavu sve većeg broja i vrsta parazita koji su otporni na lekove. Razvoj rezistencije na sve tri grupe antihelmintika širokog spektra (nikotinski antihelmintici, benzimidazoli i makrolidni laktoni) i povećana briga za zdravlje potrošača uslovljene pojavom rezidua primijenjenih lijekova u hrani, dodatno komplikuju kontrolu ovih nematoda. U cilju smanjenja brzine pojave rezistencije na antihelmintike kod gastrointestinalnih nematoda malih preživara i organizacije procesa odživog integrisanog upravljanja parazitima, globalno je prihvaćen princip ciljanog selektivnog tretmana. Sprovođenje ove strategije je tek odnedavno postalo izvodljivo, sa razvojem i praktičnom primenom sistema koji služi za kliničku procjenu anemije kod malih preživara obolelih od hemonhoze. Pored toga, kratkotrajne promene telesne težine i određivanje telesne kondicije mogu biti pokazatelji endoparazitoza, što omogućava brzu identifikaciju životinja koje će verovatno imati koristi od tretmana. Dobijeni rezultati kvantitativne koprološke dijagnostike i rezultati procene anemije su kriterijumi koji omogućavaju diferencijalnu dijagnostiku između zdravih i rezilijentnih životinja i lakšu dijagnostiku rezistencije parazita. S obzirom na to da rezilijentne životinje igraju važnu ulogu u kontaminaciji pašnjaka, razumljiv je kliničko-parazitološki značaj njihovog pravovremenog otkrivanja.
AB  - Control of the gastrointestinal parasites in different systems of ruminant breeding is based on vaccination, chemotherapy, improved herd management and use of genetic potentials of host animals. Strategy of the helminths control based on frequent anthelmintics usage is dominant among the world, although it is considered unsustainable due to the appearance of increased number and species of parasites that are resistance to drugs. Development of resistance on all three groups of broad-spectrum anthelmintics (nicotinic anthelmintics, benzimidazoles and macrolides lactones) as well as increased care for consumers health caused by appearance of used drugs residues in food, additionally complicate overall nematode control. With the aim to decrease appearance of anthelmintics resistance of gastrointestinal nematodes of small ruminants and in order to organize process of sustainable integrated parasite management, principle of targeted selective treatment is globally accepted. Implementing of this strategy has only recently become feasible, with development and practical use of systems that serve for clinical assessment of anemia in small ruminants which suffer from hemonchosis. Besides that, short term changes in body weight and body condition scoring may be indicators of diseases caused by endoparasites, as it can provide rapid identification of aminals that will probably have benefits from therapy. Obtained results of quantitative coprological diagnostic tests and results for anemia assessment are criteria that provide differential diagnosis between healthy and resilient animals and easier diseases diagnostic. Since resilient animals play important role in pasture contamination, the significance of their detection in understandable.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara
T1  - The importance of differntial diagnosis of resistance and resilience in ruminants
VL  - 19
IS  - 2
SP  - 228
EP  - 250
DO  - 10.7251/VETJSR1902228I
ER  - 

@article{
author = "Ilić, Tamara and Kulišić, Zoran and Despotović, Darko and Gajić, Bojan and Bogunović, Danica and Dimitrijević, Sanda",
year = "2019",
abstract = "Kontrola gastrointestinalnih parazita u različitim sistemima uzgoja preživara zasnivana je na vakcinaciji, hemoterapiji, poboljšanju kvaliteta menadžmenta stada i korišćenju genetskih potencijala domaćina. Širom sveta dominira strategija kontrole helminata zasnovana na čestoj upotrebi antihelmintika, koja se smatra neodrživom, s obzirom na pojavu sve većeg broja i vrsta parazita koji su otporni na lekove. Razvoj rezistencije na sve tri grupe antihelmintika širokog spektra (nikotinski antihelmintici, benzimidazoli i makrolidni laktoni) i povećana briga za zdravlje potrošača uslovljene pojavom rezidua primijenjenih lijekova u hrani, dodatno komplikuju kontrolu ovih nematoda. U cilju smanjenja brzine pojave rezistencije na antihelmintike kod gastrointestinalnih nematoda malih preživara i organizacije procesa odživog integrisanog upravljanja parazitima, globalno je prihvaćen princip ciljanog selektivnog tretmana. Sprovođenje ove strategije je tek odnedavno postalo izvodljivo, sa razvojem i praktičnom primenom sistema koji služi za kliničku procjenu anemije kod malih preživara obolelih od hemonhoze. Pored toga, kratkotrajne promene telesne težine i određivanje telesne kondicije mogu biti pokazatelji endoparazitoza, što omogućava brzu identifikaciju životinja koje će verovatno imati koristi od tretmana. Dobijeni rezultati kvantitativne koprološke dijagnostike i rezultati procene anemije su kriterijumi koji omogućavaju diferencijalnu dijagnostiku između zdravih i rezilijentnih životinja i lakšu dijagnostiku rezistencije parazita. S obzirom na to da rezilijentne životinje igraju važnu ulogu u kontaminaciji pašnjaka, razumljiv je kliničko-parazitološki značaj njihovog pravovremenog otkrivanja., Control of the gastrointestinal parasites in different systems of ruminant breeding is based on vaccination, chemotherapy, improved herd management and use of genetic potentials of host animals. Strategy of the helminths control based on frequent anthelmintics usage is dominant among the world, although it is considered unsustainable due to the appearance of increased number and species of parasites that are resistance to drugs. Development of resistance on all three groups of broad-spectrum anthelmintics (nicotinic anthelmintics, benzimidazoles and macrolides lactones) as well as increased care for consumers health caused by appearance of used drugs residues in food, additionally complicate overall nematode control. With the aim to decrease appearance of anthelmintics resistance of gastrointestinal nematodes of small ruminants and in order to organize process of sustainable integrated parasite management, principle of targeted selective treatment is globally accepted. Implementing of this strategy has only recently become feasible, with development and practical use of systems that serve for clinical assessment of anemia in small ruminants which suffer from hemonchosis. Besides that, short term changes in body weight and body condition scoring may be indicators of diseases caused by endoparasites, as it can provide rapid identification of aminals that will probably have benefits from therapy. Obtained results of quantitative coprological diagnostic tests and results for anemia assessment are criteria that provide differential diagnosis between healthy and resilient animals and easier diseases diagnostic. Since resilient animals play important role in pasture contamination, the significance of their detection in understandable.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara, The importance of differntial diagnosis of resistance and resilience in ruminants",
volume = "19",
number = "2",
pages = "228-250",
doi = "10.7251/VETJSR1902228I"
}

Ilić, T., Kulišić, Z., Despotović, D., Gajić, B., Bogunović, D.,& Dimitrijević, S.. (2019). Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 19(2), 228-250.
https://doi.org/10.7251/VETJSR1902228I

Ilić T, Kulišić Z, Despotović D, Gajić B, Bogunović D, Dimitrijević S. Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara. in Veterinarski žurnal Republike Srpske. 2019;19(2):228-250.
doi:10.7251/VETJSR1902228I .

Ilić, Tamara, Kulišić, Zoran, Despotović, Darko, Gajić, Bojan, Bogunović, Danica, Dimitrijević, Sanda, "Značaj diferencijalne dijagnostike rezistencije i rezilijencije kod preživara" in Veterinarski žurnal Republike Srpske, 19, no. 2 (2019):228-250,
https://doi.org/10.7251/VETJSR1902228I . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Nišavić, Jakov
AU  - Radojičić, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1781
AB  - Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia
VL  - 39
IS  - 3
SP  - 433
EP  - 437
DO  - 10.29261/pakvetj/2019.034
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Valčić, Miroslav and Stević, Nataša and Nišavić, Jakov and Radojičić, Sonja",
year = "2019",
abstract = "Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia",
volume = "39",
number = "3",
pages = "433-437",
doi = "10.29261/pakvetj/2019.034"
}

Milovanović, M., Milićević, V., Valčić, M., Stević, N., Nišavić, J.,& Radojičić, S.. (2019). Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 39(3), 433-437.
https://doi.org/10.29261/pakvetj/2019.034

Milovanović M, Milićević V, Valčić M, Stević N, Nišavić J, Radojičić S. Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal. 2019;39(3):433-437.
doi:10.29261/pakvetj/2019.034 .

Milovanović, Milovan, Milićević, Vesna, Valčić, Miroslav, Stević, Nataša, Nišavić, Jakov, Radojičić, Sonja, "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia" in Pakistan Veterinary Journal, 39, no. 3 (2019):433-437,
https://doi.org/10.29261/pakvetj/2019.034 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Dietze, Klaas
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Moritz, Tom
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1732
AB  - BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
VL  - 15
SP  - 80
DO  - 10.1186/s12917-019-1831-y
ER  - 

@article{
author = "Milovanović, Milovan and Dietze, Klaas and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Moritz, Tom and Hoffmann, Bernd",
year = "2019",
abstract = "BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests",
volume = "15",
pages = "80",
doi = "10.1186/s12917-019-1831-y"
}

Milovanović, M., Dietze, K., Milićević, V., Radojičić, S., Valčić, M., Moritz, T.,& Hoffmann, B.. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research
BMC, London., 15, 80.
https://doi.org/10.1186/s12917-019-1831-y

Milovanović M, Dietze K, Milićević V, Radojičić S, Valčić M, Moritz T, Hoffmann B. Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research. 2019;15:80.
doi:10.1186/s12917-019-1831-y .

Milovanović, Milovan, Dietze, Klaas, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Moritz, Tom, Hoffmann, Bernd, "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests" in BMC Veterinary Research, 15 (2019):80,
https://doi.org/10.1186/s12917-019-1831-y . .

TY  - CONF
AU  - Stajković, Silvana
AU  - Vasilev, Dragan
AU  - Teodorović, Vlado
AU  - Karabasil, Nedjeljko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1681
AB  - After an animal is harvested for meat, the skeletal muscle initiates a myriad of biochemical pathways in an attempt to maintain energy homeostasis. Anaerobic glycolysis is responsible for the generation of ATP to help meet energy demand and for the decrease in pH by generating H+. Both the rate and the extent of the post-mortem pH decline are paramount in the context of the development of pork quality attributes, such as color, water holding capacity, and texture. Pale, soft and exudative meat and dark, firm, and dry meat are two of the major quality defects facing the pork meat industry. Because glycolysis has the potential to affect meat quality attributes either positively or negatively, evaluating its regulation post-mortem is fundamental to understanding meat quality. Therefore, the aim of this study was to evaluate factors that affect mechanism of glycolysis. Special consideration will be given to meat quality attributes and development of pork quality defects.
PB  - Iop Publishing Ltd, Bristol
C3  - 60th International Meat Industry Conference (MEATCON2019)
T1  - Postmortem glycolysis and pork quality
VL  - 333
SP  - UNSP 012032
DO  - 10.1088/1755-1315/333/1/012032
ER  - 

@conference{
author = "Stajković, Silvana and Vasilev, Dragan and Teodorović, Vlado and Karabasil, Nedjeljko",
year = "2019",
abstract = "After an animal is harvested for meat, the skeletal muscle initiates a myriad of biochemical pathways in an attempt to maintain energy homeostasis. Anaerobic glycolysis is responsible for the generation of ATP to help meet energy demand and for the decrease in pH by generating H+. Both the rate and the extent of the post-mortem pH decline are paramount in the context of the development of pork quality attributes, such as color, water holding capacity, and texture. Pale, soft and exudative meat and dark, firm, and dry meat are two of the major quality defects facing the pork meat industry. Because glycolysis has the potential to affect meat quality attributes either positively or negatively, evaluating its regulation post-mortem is fundamental to understanding meat quality. Therefore, the aim of this study was to evaluate factors that affect mechanism of glycolysis. Special consideration will be given to meat quality attributes and development of pork quality defects.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "60th International Meat Industry Conference (MEATCON2019)",
title = "Postmortem glycolysis and pork quality",
volume = "333",
pages = "UNSP 012032",
doi = "10.1088/1755-1315/333/1/012032"
}

Stajković, S., Vasilev, D., Teodorović, V.,& Karabasil, N.. (2019). Postmortem glycolysis and pork quality. in 60th International Meat Industry Conference (MEATCON2019)
Iop Publishing Ltd, Bristol., 333, UNSP 012032.
https://doi.org/10.1088/1755-1315/333/1/012032

Stajković S, Vasilev D, Teodorović V, Karabasil N. Postmortem glycolysis and pork quality. in 60th International Meat Industry Conference (MEATCON2019). 2019;333:UNSP 012032.
doi:10.1088/1755-1315/333/1/012032 .

Stajković, Silvana, Vasilev, Dragan, Teodorović, Vlado, Karabasil, Nedjeljko, "Postmortem glycolysis and pork quality" in 60th International Meat Industry Conference (MEATCON2019), 333 (2019):UNSP 012032,
https://doi.org/10.1088/1755-1315/333/1/012032 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Mišić, Dušan
AU  - Karabasil, Nedjeljko
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Jevtić, Goran
AU  - Ćirić, Jelena
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1756
AB  - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Clostridium botulinum spores in European honey bees from Serbia
VL  - 58
IS  - 3
SP  - 420
EP  - 426
DO  - 10.1080/00218839.2018.1560654
ER  - 

@article{
author = "Matović, Kazimir and Mišić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena",
year = "2019",
abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Clostridium botulinum spores in European honey bees from Serbia",
volume = "58",
number = "3",
pages = "420-426",
doi = "10.1080/00218839.2018.1560654"
}

Matović, K., Mišić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 58(3), 420-426.
https://doi.org/10.1080/00218839.2018.1560654

Matović K, Mišić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426.
doi:10.1080/00218839.2018.1560654 .

Matović, Kazimir, Mišić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426,
https://doi.org/10.1080/00218839.2018.1560654 . .

TY  - JOUR
AU  - Bogunović, Danica
AU  - Stević, Nataša
AU  - Sidi-Boumedine, Karim
AU  - Mišić, Dušan
AU  - Tomanović, Snežana
AU  - Kulišić, Zoran
AU  - Magaš, Vladimir
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1589
AB  - Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)
VL  - 68
IS  - 3
SP  - 257
EP  - 268
DO  - 10.2478/acve-2018-0023
ER  - 

@article{
author = "Bogunović, Danica and Stević, Nataša and Sidi-Boumedine, Karim and Mišić, Dušan and Tomanović, Snežana and Kulišić, Zoran and Magaš, Vladimir and Radojičić, Sonja",
year = "2018",
abstract = "Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)",
volume = "68",
number = "3",
pages = "257-268",
doi = "10.2478/acve-2018-0023"
}

Bogunović, D., Stević, N., Sidi-Boumedine, K., Mišić, D., Tomanović, S., Kulišić, Z., Magaš, V.,& Radojičić, S.. (2018). Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 257-268.
https://doi.org/10.2478/acve-2018-0023

Bogunović D, Stević N, Sidi-Boumedine K, Mišić D, Tomanović S, Kulišić Z, Magaš V, Radojičić S. Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd. 2018;68(3):257-268.
doi:10.2478/acve-2018-0023 .

Bogunović, Danica, Stević, Nataša, Sidi-Boumedine, Karim, Mišić, Dušan, Tomanović, Snežana, Kulišić, Zoran, Magaš, Vladimir, Radojičić, Sonja, "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)" in Acta Veterinaria-Beograd, 68, no. 3 (2018):257-268,
https://doi.org/10.2478/acve-2018-0023 . .

TY  - CONF
AU  - Stajković, Silvana
AU  - Karabasil, Nedjeljko
AU  - Vasilev, Dragan
AU  - Dimitrijević, Mirjana
AU  - Savić Radovanović, Radoslava
AU  - Teodorović, Vlado
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3064
AB  - Валидација метода је кључан и неропходан елемент за примену метода
испитивања у лабораторији и представља процес верификације да су
специфицирани захтеви адекватни за предвиђено коришћење. Резултати
спроведене валидације метода могу се користити за процену квалитета,
поузданости и тачности аналитичких резултата; то је саставни део добре
аналитичке праксе. Лабораторија мора да валидује нестандардне методе,
методе развијене у лабораторији и стандардне методе које се користе изван
њиховог предвиђеног предмета и подручја примене, или су модификоване на
неки други начин. Укључивање одређених параметара валидације зависи од
примене методе, врсте узорака, циља методе и домаћих или међународних
смерница или прописа. Већина аналитичара је свесна значаја валидације, али
које параметре би требало укључити у протокол валидације представља
недоумицу. Поступак акредитације и контрола квалитета наглашавају
неопходност одговарајућих метода за процену несигурности мерења.
Препозната је важност процене мерне несигурности и следљивости како би се
осигурала поузданост аналитичких испитивања. Када се вреднује мерна
несигурност, сви доприноси који су значајни, морају да се узму у обзир,
коришћењем одговарајућих метода анализе. Стога је неопходно да постоји
процедура која се може лако имплементирати, која је разумљива и опште
прихваћена за карактеризацију квалитета резултата мерења, односно за
оцењивање и изражавање њене несигурности. Циљ овог рада је да се нагласи
важност валидације метода испитивања и процене мерне несигурности и да се
опишу неопходни елементи који су опште прихваћени у поступку валидације и
процене мерне несигурности.
AB  - Method validation is an essential and necessary element in the implementation of
any laboratory analytical method and reprezent a process of verification where the
specified requirements are adequate for an intended use. Results from method
validation can be used to judge the quality, reliability and consistency of analytical
results; it is an integral part of any good analytical practice. The laboratory shall
validate non-standard methods, laboratory-developed methods and standard
methods used outside their intended scope or otherwise modified. The inclusion of
particular validation parameters in a validation protocol depends on the
application, the test samples, the goal of the method, and domestic or international
guidelines or regulations, as applicable. Most analysts are aware of its importance,
but what parameters needs to be included in validation protocol is not always clear.
The implementation of accreditation and quality control procedures emphasizes
the necessity of suitable methods for estimating the measurement uncertainty.
It is well recognized the importance of measurement uncertainty and traceability
to ensure the reliability of analytical assays. When evaluating measurement
uncertainty, all contributions that are of significance, shall be taken into account
using appropriate methods of analysis. It is therefore necessary that there be a
readily implemented, easily understood, and generally accepted procedure for
characterizing the quality of a result of a measurement, that is, for evaluating and
expressing its
uncertainty. The goal of this paper is to emphasize the importance of validation of
analytical methods and estimating the measurement uncertainty and to describe
set of elements that are generally accepted as critical for an analytical method
validation and for estimating the measurement uncertainty
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
T1  - Validacija metoda ispitivanja hrane i procena merne nesigurnosti – značaj i principi
T1  - Validation of food analytical methods and measurement uncertainty – singficance and principles
SP  - 105
EP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3064
ER  - 

@conference{
author = "Stajković, Silvana and Karabasil, Nedjeljko and Vasilev, Dragan and Dimitrijević, Mirjana and Savić Radovanović, Radoslava and Teodorović, Vlado",
year = "2018",
abstract = "Валидација метода је кључан и неропходан елемент за примену метода
испитивања у лабораторији и представља процес верификације да су
специфицирани захтеви адекватни за предвиђено коришћење. Резултати
спроведене валидације метода могу се користити за процену квалитета,
поузданости и тачности аналитичких резултата; то је саставни део добре
аналитичке праксе. Лабораторија мора да валидује нестандардне методе,
методе развијене у лабораторији и стандардне методе које се користе изван
њиховог предвиђеног предмета и подручја примене, или су модификоване на
неки други начин. Укључивање одређених параметара валидације зависи од
примене методе, врсте узорака, циља методе и домаћих или међународних
смерница или прописа. Већина аналитичара је свесна значаја валидације, али
које параметре би требало укључити у протокол валидације представља
недоумицу. Поступак акредитације и контрола квалитета наглашавају
неопходност одговарајућих метода за процену несигурности мерења.
Препозната је важност процене мерне несигурности и следљивости како би се
осигурала поузданост аналитичких испитивања. Када се вреднује мерна
несигурност, сви доприноси који су значајни, морају да се узму у обзир,
коришћењем одговарајућих метода анализе. Стога је неопходно да постоји
процедура која се може лако имплементирати, која је разумљива и опште
прихваћена за карактеризацију квалитета резултата мерења, односно за
оцењивање и изражавање њене несигурности. Циљ овог рада је да се нагласи
важност валидације метода испитивања и процене мерне несигурности и да се
опишу неопходни елементи који су опште прихваћени у поступку валидације и
процене мерне несигурности., Method validation is an essential and necessary element in the implementation of
any laboratory analytical method and reprezent a process of verification where the
specified requirements are adequate for an intended use. Results from method
validation can be used to judge the quality, reliability and consistency of analytical
results; it is an integral part of any good analytical practice. The laboratory shall
validate non-standard methods, laboratory-developed methods and standard
methods used outside their intended scope or otherwise modified. The inclusion of
particular validation parameters in a validation protocol depends on the
application, the test samples, the goal of the method, and domestic or international
guidelines or regulations, as applicable. Most analysts are aware of its importance,
but what parameters needs to be included in validation protocol is not always clear.
The implementation of accreditation and quality control procedures emphasizes
the necessity of suitable methods for estimating the measurement uncertainty.
It is well recognized the importance of measurement uncertainty and traceability
to ensure the reliability of analytical assays. When evaluating measurement
uncertainty, all contributions that are of significance, shall be taken into account
using appropriate methods of analysis. It is therefore necessary that there be a
readily implemented, easily understood, and generally accepted procedure for
characterizing the quality of a result of a measurement, that is, for evaluating and
expressing its
uncertainty. The goal of this paper is to emphasize the importance of validation of
analytical methods and estimating the measurement uncertainty and to describe
set of elements that are generally accepted as critical for an analytical method
validation and for estimating the measurement uncertainty",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.",
title = "Validacija metoda ispitivanja hrane i procena merne nesigurnosti – značaj i principi, Validation of food analytical methods and measurement uncertainty – singficance and principles",
pages = "105-106",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3064"
}

Stajković, S., Karabasil, N., Vasilev, D., Dimitrijević, M., Savić Radovanović, R.,& Teodorović, V.. (2018). Validacija metoda ispitivanja hrane i procena merne nesigurnosti – značaj i principi. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.
Banja Luka : Veterinarska komora Republike Srpske., 105-106.
https://hdl.handle.net/21.15107/rcub_veterinar_3064

Stajković S, Karabasil N, Vasilev D, Dimitrijević M, Savić Radovanović R, Teodorović V. Validacija metoda ispitivanja hrane i procena merne nesigurnosti – značaj i principi. in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018.. 2018;:105-106.
https://hdl.handle.net/21.15107/rcub_veterinar_3064 .

Stajković, Silvana, Karabasil, Nedjeljko, Vasilev, Dragan, Dimitrijević, Mirjana, Savić Radovanović, Radoslava, Teodorović, Vlado, "Validacija metoda ispitivanja hrane i procena merne nesigurnosti – značaj i principi" in 23. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 6-9. jun 2018. (2018):105-106,
https://hdl.handle.net/21.15107/rcub_veterinar_3064 .

TY  - THES
AU  - Stević, Nataša
PY  - 2018
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50175247
UR  - http://nardus.mpn.gov.rs/123456789/9553
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/44
AB  - The scientific community has known about canine brucellosis for over four decades, even so, there are no standardized diagnostic protocols, nor a general agreement on the most appropriate diagnostic test. Each laboratory defines its own criteria. This variety of tests and the lack of clearly defined protocols lead to difficulties in interpreting the results of serological tests in different laboratories. For this reason, the goal of this doctoral dissertation has been to improve diagnostics using recommended and new, self-prepared tests. One of the tasks of this dissertation was to examine the usability of the Bruce-ladder multiplex PCR method for testing clinical samples, i.e. dog uterine and testicular tissues. The material (blood, testicles and uteruses) was collected from 225 dogs without owners, 145 female and 80 male dogs. The results obtained showed that from a total of 225 tested samples, 33 or 14,67% of blood sera had measurable antibody titer in 2-ME TAT. 13 or 5,78% blood sera had the lowest tested titer of 1/50, in 8 or 3,55% blood sera a titer of 1/100 was determined, while 12 serum samples or 5,33% had the titer of 1/200. By applying classic bacteriological methods, B. canis was isolated from three samples of homogenized reproductive organ tissue (1,33%), 2 from samples originating from males and one specimen originating from a female. One isolate originated from a serologically negative dog. Of the 225 samples assayed using the Bruce-ladder multiplex PCR method, a positive reaction was established in two (0,88%). Protein concentrations were determined prior to the formulation of the indirect ELISA test. The electrophoretic analysis of antigens retrieved using heat and ultrasound, as well as the densitometric quantification, showed that the antigen retrieved using heat had the most prevalent molecular weight fraction of 10,95 kDa with a participation of as much as 43,12% that corresponds to Brucella R LPS. The same fraction was represented in the antigen retrieved using ultrasound with 11,56%, or in a quantity that was 3,7 times lower...
AB  - Bruceloza pasa je bolest poznata preko četiri decenije, ali i pored toga ne postoje standardizovani dijagnostički protokoli, kao ni generalni dogovor o najprikladnijem dijagnostičkom testu. Svaka laboratorija definiše sopstvene kriterijume. Ovakva raznovrsnost testova i nedostatak jasno definisanih protokola dovodi do teškoća u interpretaciji rezultata seroloških testova u različitim laboratorijama. Iz tog razloga, cilj ove doktorske disertacije obuhvatio je unapređenje dijagnostike primenom preporučenih i novih, sopstveno pripremljenih testova. Jedan od zadataka ove disertacije je bilo i ispitivanje upotrebljivosti Bruce-ladder multiplex PCR metode za ispitivanje kliničkih uzoraka odnosno tkiva uterusa i testisa pasa. Sakupljen je materijal (krv, testisi i materice) od 225 nevlasničkih pasa i to 145 ženki i 80 mužjaka. Dobijeni rezultati su pokazali da je od ukupno 225 ispitanih uzoraka, 33 ili 14,67% krvnih seruma imalo merljiv titar antitela u 2-ME TAT. Najniži ispitivani titar od 1/50 imalo je 13 krvnih seruma ili 5,78%, kod 8 krvnih seruma ustanovljen je titar od 1/100 ili 3,55%, dok je titar od 1/200 imalo 12 uzoraka seruma ili 5,33%. Primenom klasičnih bakterioloških metoda, B. canis je izolovana iz tri uzorka homogenizata tkiva reproduktivnih organa (1,33%) i to iz 2 uzorka poreklom od mužjaka i jednog uzorka poreklom od ženke. Jedan izolat je poticao od serološki negativnog psa. Od 225 uzoraka ispitanih Bruce-ladder multiplex PCR metodom, pozitivna reakcija je ustanovljena kod dva (0,88%). Pre formulacije indirektnog ELISA testa, određivane su koncentracije proteina. Elektroforetska analiza antigena dobijenih toplotom i ultrazvukom kao i denzitometrijska kvantifikacija su pokazale da je u antigenu dobijenom toplotom, najzastupljenija frakcija molekulske mase 10,95 kDa sa učešćem od čak 43,12% koja odgovara R-LPS-u brucela. Ista frakcija je u antigenu dobijenom ultrazvukom bila zastupljena sa 11,56%, odnosno u količini koja je bila 3,7x manja...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Examination of the reliability of serological, bacteriological and molecular methods in the diagnosis of canine brucellosis caused by Brucella canis
T1  - Ispitivanje pouzdanosti seroloških, bakterioloških i molekularnih metoda u dijagnostici bruceloze pasa izazvane vrstom Brucella canis
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9553
ER  - 

@phdthesis{
author = "Stević, Nataša",
year = "2018",
abstract = "The scientific community has known about canine brucellosis for over four decades, even so, there are no standardized diagnostic protocols, nor a general agreement on the most appropriate diagnostic test. Each laboratory defines its own criteria. This variety of tests and the lack of clearly defined protocols lead to difficulties in interpreting the results of serological tests in different laboratories. For this reason, the goal of this doctoral dissertation has been to improve diagnostics using recommended and new, self-prepared tests. One of the tasks of this dissertation was to examine the usability of the Bruce-ladder multiplex PCR method for testing clinical samples, i.e. dog uterine and testicular tissues. The material (blood, testicles and uteruses) was collected from 225 dogs without owners, 145 female and 80 male dogs. The results obtained showed that from a total of 225 tested samples, 33 or 14,67% of blood sera had measurable antibody titer in 2-ME TAT. 13 or 5,78% blood sera had the lowest tested titer of 1/50, in 8 or 3,55% blood sera a titer of 1/100 was determined, while 12 serum samples or 5,33% had the titer of 1/200. By applying classic bacteriological methods, B. canis was isolated from three samples of homogenized reproductive organ tissue (1,33%), 2 from samples originating from males and one specimen originating from a female. One isolate originated from a serologically negative dog. Of the 225 samples assayed using the Bruce-ladder multiplex PCR method, a positive reaction was established in two (0,88%). Protein concentrations were determined prior to the formulation of the indirect ELISA test. The electrophoretic analysis of antigens retrieved using heat and ultrasound, as well as the densitometric quantification, showed that the antigen retrieved using heat had the most prevalent molecular weight fraction of 10,95 kDa with a participation of as much as 43,12% that corresponds to Brucella R LPS. The same fraction was represented in the antigen retrieved using ultrasound with 11,56%, or in a quantity that was 3,7 times lower..., Bruceloza pasa je bolest poznata preko četiri decenije, ali i pored toga ne postoje standardizovani dijagnostički protokoli, kao ni generalni dogovor o najprikladnijem dijagnostičkom testu. Svaka laboratorija definiše sopstvene kriterijume. Ovakva raznovrsnost testova i nedostatak jasno definisanih protokola dovodi do teškoća u interpretaciji rezultata seroloških testova u različitim laboratorijama. Iz tog razloga, cilj ove doktorske disertacije obuhvatio je unapređenje dijagnostike primenom preporučenih i novih, sopstveno pripremljenih testova. Jedan od zadataka ove disertacije je bilo i ispitivanje upotrebljivosti Bruce-ladder multiplex PCR metode za ispitivanje kliničkih uzoraka odnosno tkiva uterusa i testisa pasa. Sakupljen je materijal (krv, testisi i materice) od 225 nevlasničkih pasa i to 145 ženki i 80 mužjaka. Dobijeni rezultati su pokazali da je od ukupno 225 ispitanih uzoraka, 33 ili 14,67% krvnih seruma imalo merljiv titar antitela u 2-ME TAT. Najniži ispitivani titar od 1/50 imalo je 13 krvnih seruma ili 5,78%, kod 8 krvnih seruma ustanovljen je titar od 1/100 ili 3,55%, dok je titar od 1/200 imalo 12 uzoraka seruma ili 5,33%. Primenom klasičnih bakterioloških metoda, B. canis je izolovana iz tri uzorka homogenizata tkiva reproduktivnih organa (1,33%) i to iz 2 uzorka poreklom od mužjaka i jednog uzorka poreklom od ženke. Jedan izolat je poticao od serološki negativnog psa. Od 225 uzoraka ispitanih Bruce-ladder multiplex PCR metodom, pozitivna reakcija je ustanovljena kod dva (0,88%). Pre formulacije indirektnog ELISA testa, određivane su koncentracije proteina. Elektroforetska analiza antigena dobijenih toplotom i ultrazvukom kao i denzitometrijska kvantifikacija su pokazale da je u antigenu dobijenom toplotom, najzastupljenija frakcija molekulske mase 10,95 kDa sa učešćem od čak 43,12% koja odgovara R-LPS-u brucela. Ista frakcija je u antigenu dobijenom ultrazvukom bila zastupljena sa 11,56%, odnosno u količini koja je bila 3,7x manja...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Examination of the reliability of serological, bacteriological and molecular methods in the diagnosis of canine brucellosis caused by Brucella canis, Ispitivanje pouzdanosti seroloških, bakterioloških i molekularnih metoda u dijagnostici bruceloze pasa izazvane vrstom Brucella canis",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9553"
}

Stević, N.. (2018). Examination of the reliability of serological, bacteriological and molecular methods in the diagnosis of canine brucellosis caused by Brucella canis. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_9553

Stević N. Examination of the reliability of serological, bacteriological and molecular methods in the diagnosis of canine brucellosis caused by Brucella canis. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_9553 .

Stević, Nataša, "Examination of the reliability of serological, bacteriological and molecular methods in the diagnosis of canine brucellosis caused by Brucella canis" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_9553 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Kureljušić, Jasna
AU  - Šekler, Milanko
AU  - Nešić, Ksenija
AU  - Veljović, Ljubiša
AU  - Zorić-Maksimović, Jelena
AU  - Radosavljević, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1657
AB  - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.
PB  - Springeropen, London
T2  - AMB EXPRESS
T1  - Molecular detection of black queen cell virus and Kashmir bee virus in honey
VL  - 8
SP  - 128
DO  - 10.1186/s13568-018-0655-7
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Zorić-Maksimović, Jelena and Radosavljević, Vladimir",
year = "2018",
abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.",
publisher = "Springeropen, London",
journal = "AMB EXPRESS",
title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey",
volume = "8",
pages = "128",
doi = "10.1186/s13568-018-0655-7"
}

Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Zorić-Maksimović, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS
Springeropen, London., 8, 128.
https://doi.org/10.1186/s13568-018-0655-7

Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Zorić-Maksimović J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS. 2018;8:128.
doi:10.1186/s13568-018-0655-7 .

Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Zorić-Maksimović, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB EXPRESS, 8 (2018):128,
https://doi.org/10.1186/s13568-018-0655-7 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Ćirić, Jelena
AU  - Kaljević, Vesna
AU  - Nedić, Nebojša
AU  - Jevtić, Goran
AU  - Vasković, Nikola
AU  - Baltić, Milan Ž.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1660
AB  - Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia
VL  - 25
IS  - 14
SP  - 14148
EP  - 14157
DO  - 10.1007/s11356-018-1659-1
ER  - 

@article{
author = "Matović, Kazimir and Ćirić, Jelena and Kaljević, Vesna and Nedić, Nebojša and Jevtić, Goran and Vasković, Nikola and Baltić, Milan Ž.",
year = "2018",
abstract = "Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia",
volume = "25",
number = "14",
pages = "14148-14157",
doi = "10.1007/s11356-018-1659-1"
}

Matović, K., Ćirić, J., Kaljević, V., Nedić, N., Jevtić, G., Vasković, N.,& Baltić, M. Ž.. (2018). Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 25(14), 14148-14157.
https://doi.org/10.1007/s11356-018-1659-1

Matović K, Ćirić J, Kaljević V, Nedić N, Jevtić G, Vasković N, Baltić MŽ. Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research. 2018;25(14):14148-14157.
doi:10.1007/s11356-018-1659-1 .

Matović, Kazimir, Ćirić, Jelena, Kaljević, Vesna, Nedić, Nebojša, Jevtić, Goran, Vasković, Nikola, Baltić, Milan Ž., "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia" in Environmental Science and Pollution Research, 25, no. 14 (2018):14148-14157,
https://doi.org/10.1007/s11356-018-1659-1 . .

TY  - JOUR
AU  - Stević, Nataša
AU  - Mišić, Dušan
AU  - Bogunović, Danica
AU  - Matović, Kazimir
AU  - Valčić, Miroslav
AU  - Milovanović, Milovan
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1544
AB  - The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay
VL  - 67
IS  - 4
SP  - 551
EP  - 561
DO  - 10.1515/acve-2017-0046
ER  - 

@article{
author = "Stević, Nataša and Mišić, Dušan and Bogunović, Danica and Matović, Kazimir and Valčić, Miroslav and Milovanović, Milovan and Radojičić, Sonja",
year = "2017",
abstract = "The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay",
volume = "67",
number = "4",
pages = "551-561",
doi = "10.1515/acve-2017-0046"
}

Stević, N., Mišić, D., Bogunović, D., Matović, K., Valčić, M., Milovanović, M.,& Radojičić, S.. (2017). Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 551-561.
https://doi.org/10.1515/acve-2017-0046

Stević N, Mišić D, Bogunović D, Matović K, Valčić M, Milovanović M, Radojičić S. Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd. 2017;67(4):551-561.
doi:10.1515/acve-2017-0046 .

Stević, Nataša, Mišić, Dušan, Bogunović, Danica, Matović, Kazimir, Valčić, Miroslav, Milovanović, Milovan, Radojičić, Sonja, "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay" in Acta Veterinaria-Beograd, 67, no. 4 (2017):551-561,
https://doi.org/10.1515/acve-2017-0046 . .

TY  - JOUR
AU  - Stević, Nataša
AU  - Milovanović, Milovan
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2155
AB  - Kada se bruceloza ustanovi u jednoj zemlji, međunarodni veterinarski propisi
nameću ograničenja kretanja životinja i trgovine, što dovodi do velikih ekonomskih
gubitaka. Izolacija Brucella spp. konvencionalnim bakteriološkim tehnikama
je dugotrajna, rizična za laboratorijske radnike i niske osetljivosti zbog česte
kontaminacije materijala. Negativna izolacija ne isključuje postojanje bruceloze.
Najbolji rezultati do sada su dobijeni kombinovanjem metoda izolacije i PCR metode
na kliničkim uzorcima. Nedostatak PCR metoda baziranih na razlici među sojevima
unutar vrste stimulisao je razvoj novih tehnika „otisaka prstiju”. Podaci o sekvenci
celog genoma brucela omogućili su identifikaciju i razlikovanje brucela na nivou vrste,
biovara i upoređivanje sojeva što je olakšalo pronalaženje izvora infekcije. Indirektni
dijagnostički testovi su zasnovani na detekciji imunskog odgovora izazvanog
infekcijom. Ovi testovi pokazuju različit stepen osetljivosti i specifičnosti zavisno od
brojnih varijabli, poput stepena i načina inficiranja, prisustva takozvanih „unakrsno
reaktivnih bakterija” antigenski sličnih Brucella spp, kinetike indukovanog imunskog
odgovora i prethodne vakcinacije. Imajući u vidu kinetiku nastajanja imunskog
odgovora indukovanog nakon infekcije, vreme kada će se različiti testovi izvoditi ima
veliki uticaj na rezultate.
AB  - When brucellosis is detected in a country, international veterinary regulations impose
restrictions on animal movements and trade, which result in huge economic losses.
The isolation of Brucella spp. through conventional bacteriological techniques takes
a long time, it’s risky for the laboratory workers, and has low sensitivity due to
the frequent contamination of materials. Negative cultures do not rule out the disease.
The best results have so far been obtained by combining culture and PCR detection on
clinical samples. The lack of PCR-based methods for differentiation among strains within
a species stimulate the development of new techniques-”fingerprinting methods”.
Data on the sequence of the genome of Brucella enabled the identification and differentiation
of Brucella at the level of species and biovar and comparison strains which
is easier to find the source of infection. Indirect diagnostic tests are based on the detection
of immune responses induced by infection. These tests show different sensitivities
and specificities depending on numerous variables, such as the infection dose and
route, the presence of so-called “cross-reactive bacteria” antigenically similar to Brucella
spp., the kinetics of the induced immune response, and previous vaccination. Bearing
in mind kinetics of the immune response induced after infection, the time when
the different tests carried out has a major impact on the results.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Mogućnost dijagnostike bruceloze domaćih životinja
T1  - The possibility of diagnosis of domestic animal Brucellosis
VL  - 17
IS  - 1
SP  - 93
EP  - 104
DO  - 10.7251/VETJ1701093S
ER  - 

@article{
author = "Stević, Nataša and Milovanović, Milovan and Radojičić, Sonja and Valčić, Miroslav",
year = "2017",
abstract = "Kada se bruceloza ustanovi u jednoj zemlji, međunarodni veterinarski propisi
nameću ograničenja kretanja životinja i trgovine, što dovodi do velikih ekonomskih
gubitaka. Izolacija Brucella spp. konvencionalnim bakteriološkim tehnikama
je dugotrajna, rizična za laboratorijske radnike i niske osetljivosti zbog česte
kontaminacije materijala. Negativna izolacija ne isključuje postojanje bruceloze.
Najbolji rezultati do sada su dobijeni kombinovanjem metoda izolacije i PCR metode
na kliničkim uzorcima. Nedostatak PCR metoda baziranih na razlici među sojevima
unutar vrste stimulisao je razvoj novih tehnika „otisaka prstiju”. Podaci o sekvenci
celog genoma brucela omogućili su identifikaciju i razlikovanje brucela na nivou vrste,
biovara i upoređivanje sojeva što je olakšalo pronalaženje izvora infekcije. Indirektni
dijagnostički testovi su zasnovani na detekciji imunskog odgovora izazvanog
infekcijom. Ovi testovi pokazuju različit stepen osetljivosti i specifičnosti zavisno od
brojnih varijabli, poput stepena i načina inficiranja, prisustva takozvanih „unakrsno
reaktivnih bakterija” antigenski sličnih Brucella spp, kinetike indukovanog imunskog
odgovora i prethodne vakcinacije. Imajući u vidu kinetiku nastajanja imunskog
odgovora indukovanog nakon infekcije, vreme kada će se različiti testovi izvoditi ima
veliki uticaj na rezultate., When brucellosis is detected in a country, international veterinary regulations impose
restrictions on animal movements and trade, which result in huge economic losses.
The isolation of Brucella spp. through conventional bacteriological techniques takes
a long time, it’s risky for the laboratory workers, and has low sensitivity due to
the frequent contamination of materials. Negative cultures do not rule out the disease.
The best results have so far been obtained by combining culture and PCR detection on
clinical samples. The lack of PCR-based methods for differentiation among strains within
a species stimulate the development of new techniques-”fingerprinting methods”.
Data on the sequence of the genome of Brucella enabled the identification and differentiation
of Brucella at the level of species and biovar and comparison strains which
is easier to find the source of infection. Indirect diagnostic tests are based on the detection
of immune responses induced by infection. These tests show different sensitivities
and specificities depending on numerous variables, such as the infection dose and
route, the presence of so-called “cross-reactive bacteria” antigenically similar to Brucella
spp., the kinetics of the induced immune response, and previous vaccination. Bearing
in mind kinetics of the immune response induced after infection, the time when
the different tests carried out has a major impact on the results.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Mogućnost dijagnostike bruceloze domaćih životinja, The possibility of diagnosis of domestic animal Brucellosis",
volume = "17",
number = "1",
pages = "93-104",
doi = "10.7251/VETJ1701093S"
}

Stević, N., Milovanović, M., Radojičić, S.,& Valčić, M.. (2017). Mogućnost dijagnostike bruceloze domaćih životinja. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(1), 93-104.
https://doi.org/10.7251/VETJ1701093S

Stević N, Milovanović M, Radojičić S, Valčić M. Mogućnost dijagnostike bruceloze domaćih životinja. in Veterinarski žurnal Republike Srpske. 2017;17(1):93-104.
doi:10.7251/VETJ1701093S .

Stević, Nataša, Milovanović, Milovan, Radojičić, Sonja, Valčić, Miroslav, "Mogućnost dijagnostike bruceloze domaćih životinja" in Veterinarski žurnal Republike Srpske, 17, no. 1 (2017):93-104,
https://doi.org/10.7251/VETJ1701093S . .

TY  - CONF
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Milovanoviić, Milovan
AU  - Živojinović, Milena
AU  - Milićević, Vesna
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3290
AB  - Куга малих преживара (Peste des petits ruminants-PPR) је једна у низу болести које,
последњих година, показују тенденцију ширења ван територија ензоотског појављивања. Као
таква, куга малих преживара се убраја у болести од које ризик за појаву на територији Европе
расте. Турска ову болест има дужи низ година, али и поред кампања вакцинације малих преживара
која је финансирана од стране Европске уније (ЕУ), ситуација није битно промењена. С обзиром
на то да је болест готово стално присустна у Турској, посебно је опасно њено избијање у западном
делу, у близини границе са Грчком (2013. и 2014. година). Важна је и појава болести у Грузији
2016. године; поред ње, ППР је присутна и у Јерменији, а верује се да је има и у Азербејџану и
Русији, мада нема званичних пријава болести. Са друге стране, епизоотиолошка ситуација у
северној Африци је такође погоршана због поновног избијања болести у Мароку 2015. и Тунису
2016. године. Мада се ППР може контролисати кампањама масовне вакцинације, неконтролисан,
као и нелегалан промет животиња су потенцијална опасност за њено ширење на нове земље. Иако
је у случају појаве болести у земљама ЕУ прописан stamping out, због високог ризика од уношења
заразе на територију Европе, поједини тимови научника, већ експериментишу са инактивисаном
вакцином против ове болести, која би (као и у случају болести плавог језика) била сигурна и
адекватна замена за атенуирану вакцину која се данас примењује. У том смислу, сарадња ЕУ са
осталим земљама у окружењу је неопходна како би се спречило ширење не само ППР, већ и
других, такозваних "прекограничних, претећих болести".
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 28. Savetovanje veterinara Srbije, Zlatibor, 7 - 10. septembar 2017
T1  - Aktuelna epizootiološka situacija kuge malih preživara
SP  - 73
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3290
ER  - 

@conference{
author = "Radojičić, Sonja and Valčić, Miroslav and Stević, Nataša and Milovanoviić, Milovan and Živojinović, Milena and Milićević, Vesna",
year = "2017",
abstract = "Куга малих преживара (Peste des petits ruminants-PPR) је једна у низу болести које,
последњих година, показују тенденцију ширења ван територија ензоотског појављивања. Као
таква, куга малих преживара се убраја у болести од које ризик за појаву на територији Европе
расте. Турска ову болест има дужи низ година, али и поред кампања вакцинације малих преживара
која је финансирана од стране Европске уније (ЕУ), ситуација није битно промењена. С обзиром
на то да је болест готово стално присустна у Турској, посебно је опасно њено избијање у западном
делу, у близини границе са Грчком (2013. и 2014. година). Важна је и појава болести у Грузији
2016. године; поред ње, ППР је присутна и у Јерменији, а верује се да је има и у Азербејџану и
Русији, мада нема званичних пријава болести. Са друге стране, епизоотиолошка ситуација у
северној Африци је такође погоршана због поновног избијања болести у Мароку 2015. и Тунису
2016. године. Мада се ППР може контролисати кампањама масовне вакцинације, неконтролисан,
као и нелегалан промет животиња су потенцијална опасност за њено ширење на нове земље. Иако
је у случају појаве болести у земљама ЕУ прописан stamping out, због високог ризика од уношења
заразе на територију Европе, поједини тимови научника, већ експериментишу са инактивисаном
вакцином против ове болести, која би (као и у случају болести плавог језика) била сигурна и
адекватна замена за атенуирану вакцину која се данас примењује. У том смислу, сарадња ЕУ са
осталим земљама у окружењу је неопходна како би се спречило ширење не само ППР, већ и
других, такозваних "прекограничних, претећих болести".",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "28. Savetovanje veterinara Srbije, Zlatibor, 7 - 10. septembar 2017",
title = "Aktuelna epizootiološka situacija kuge malih preživara",
pages = "73-78",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3290"
}

Radojičić, S., Valčić, M., Stević, N., Milovanoviić, M., Živojinović, M.,& Milićević, V.. (2017). Aktuelna epizootiološka situacija kuge malih preživara. in 28. Savetovanje veterinara Srbije, Zlatibor, 7 - 10. septembar 2017
Beograd : Srpsko veterinarsko društvo., 73-78.
https://hdl.handle.net/21.15107/rcub_veterinar_3290

Radojičić S, Valčić M, Stević N, Milovanoviić M, Živojinović M, Milićević V. Aktuelna epizootiološka situacija kuge malih preživara. in 28. Savetovanje veterinara Srbije, Zlatibor, 7 - 10. septembar 2017. 2017;:73-78.
https://hdl.handle.net/21.15107/rcub_veterinar_3290 .

Radojičić, Sonja, Valčić, Miroslav, Stević, Nataša, Milovanoviić, Milovan, Živojinović, Milena, Milićević, Vesna, "Aktuelna epizootiološka situacija kuge malih preživara" in 28. Savetovanje veterinara Srbije, Zlatibor, 7 - 10. septembar 2017 (2017):73-78,
https://hdl.handle.net/21.15107/rcub_veterinar_3290 .

TY  - GEN
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2633
AB  - Poznavanje i analiza trenutne epizootiološke situacije u Evropi i
regionu, postala je imperativ za blagovremenu primenu planova i podizanje spremnosti veterinarske službe u Srbiji. Pored zaraznih bolesti
koje se u našoj zemlji javljaju po prvi put, posebnu pažnju treba obratiti na one koje predstavljaju stalnu pretnju i kod kojih rizik od unošenja stalno raste. Najnovija pojava afričke kuge svinja u Moldaviji i mogućnost unošenja bolesti u Rumuniju pa i našu zemlju, ima poseban
značaj i zbog obaveze prestanka vakcinacije protiv klasične kuge svinja. Izbijanje afričke kuge svinja na farmi velikog uzgojnog kapaciteta
u Ukrajini, dokazuje da biosigurnosne mere moraju da budu na najvišem mogućem nivou, uz obavezu stalnog praćenja i posebno važnog
brzog prepoznavanja bolesti. Pored epizootije nodularnog dermatitisa i bolesti plavog jezika u 2016. godini, kao i prijave novog tipa avijarne influence H5N8 na teritoriji Republike Srbije, ne treba zaboraviti
i one bolesti koje se duži vremenski period registruju na teritoriji naše zemlje sa niskom učestalošću Ovo se pre svega odnosi na širenje
bruceloze svinja koja u poslednjih nekoliko meseci zaokuplja pažnju
stručne javnosti.
AB  - CURRENT EPIZOOTIOLOGICAL SITUATION IN SERBIA AND EUROPE
Understanding and analyzing the current epizootiological burden of communicable
diseases in the region and Europe became an imperative for timely intervention planning
and capacity building of the veterinary service in Serbia. Apart from the communicable diseases that are being diagnosed for the first time in our country, the attention still needs to
be addressed to those representing the constant threat, and also pose an ever-rising risk
for contracting. The latest outbreak of African swine fever in Moldova, and possibility of its
spread to Romania and Serbia, is of utmost importance also due to the obligatory caseation of vaccination for the classical swine fever. The outbreak of African swine fever at the
Ukrainian high-capacity breading farm shows that biosecurity needs to be at the highest
level, ensuring the obligatory disease control and fast disease identification in particular.
Furthermore, aside from epizootiology of nodular dermatitis and blue tongue disease in
2016, as well as the first recording of the new avian influenza type – H5N8 on the territory
of Serbia, the diseases being registered over the longer period of time on the territory of
our country while having a low incidence should not be forgotten. This is especially related
to the spread of swine brucellosis, which has been a focus of the experts’ attention for the
past several months
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
T2  - XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA
T1  - Aktuelna epizootiološka situacija u Srbiji i Evropi
SP  - 3
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2633
ER  - 

@misc{
author = "Radojičić, Sonja and Valčić, Miroslav",
year = "2017",
abstract = "Poznavanje i analiza trenutne epizootiološke situacije u Evropi i
regionu, postala je imperativ za blagovremenu primenu planova i podizanje spremnosti veterinarske službe u Srbiji. Pored zaraznih bolesti
koje se u našoj zemlji javljaju po prvi put, posebnu pažnju treba obratiti na one koje predstavljaju stalnu pretnju i kod kojih rizik od unošenja stalno raste. Najnovija pojava afričke kuge svinja u Moldaviji i mogućnost unošenja bolesti u Rumuniju pa i našu zemlju, ima poseban
značaj i zbog obaveze prestanka vakcinacije protiv klasične kuge svinja. Izbijanje afričke kuge svinja na farmi velikog uzgojnog kapaciteta
u Ukrajini, dokazuje da biosigurnosne mere moraju da budu na najvišem mogućem nivou, uz obavezu stalnog praćenja i posebno važnog
brzog prepoznavanja bolesti. Pored epizootije nodularnog dermatitisa i bolesti plavog jezika u 2016. godini, kao i prijave novog tipa avijarne influence H5N8 na teritoriji Republike Srbije, ne treba zaboraviti
i one bolesti koje se duži vremenski period registruju na teritoriji naše zemlje sa niskom učestalošću Ovo se pre svega odnosi na širenje
bruceloze svinja koja u poslednjih nekoliko meseci zaokuplja pažnju
stručne javnosti., CURRENT EPIZOOTIOLOGICAL SITUATION IN SERBIA AND EUROPE
Understanding and analyzing the current epizootiological burden of communicable
diseases in the region and Europe became an imperative for timely intervention planning
and capacity building of the veterinary service in Serbia. Apart from the communicable diseases that are being diagnosed for the first time in our country, the attention still needs to
be addressed to those representing the constant threat, and also pose an ever-rising risk
for contracting. The latest outbreak of African swine fever in Moldova, and possibility of its
spread to Romania and Serbia, is of utmost importance also due to the obligatory caseation of vaccination for the classical swine fever. The outbreak of African swine fever at the
Ukrainian high-capacity breading farm shows that biosecurity needs to be at the highest
level, ensuring the obligatory disease control and fast disease identification in particular.
Furthermore, aside from epizootiology of nodular dermatitis and blue tongue disease in
2016, as well as the first recording of the new avian influenza type – H5N8 on the territory
of Serbia, the diseases being registered over the longer period of time on the territory of
our country while having a low incidence should not be forgotten. This is especially related
to the spread of swine brucellosis, which has been a focus of the experts’ attention for the
past several months",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA",
title = "Aktuelna epizootiološka situacija u Srbiji i Evropi",
pages = "3-11",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2633"
}

Radojičić, S.,& Valčić, M.. (2017). Aktuelna epizootiološka situacija u Srbiji i Evropi. in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 3-11.
https://hdl.handle.net/21.15107/rcub_veterinar_2633

Radojičić S, Valčić M. Aktuelna epizootiološka situacija u Srbiji i Evropi. in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA. 2017;:3-11.
https://hdl.handle.net/21.15107/rcub_veterinar_2633 .

Radojičić, Sonja, Valčić, Miroslav, "Aktuelna epizootiološka situacija u Srbiji i Evropi" in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA (2017):3-11,
https://hdl.handle.net/21.15107/rcub_veterinar_2633 .

TY  - GEN
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Stević, Nataša
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2631
AB  - Poslednje godine prošlog veka ukazale su na trend širenja egzotičnih zaraznih bolesti koje do tada nisu imale bitan epizootiološki i
epidemiološki potencijal. Prva iznenađenja donela je groznica Zapadnog Nila, naizgled lokalna bolest malog potencijala širenja, koja se
za nepunih 10 godina iz Afrike prvo proširila na Ameriku a zatim i ceo
svet. U prvoj deceniji 21. veka, postalo je jasno da globalizacija kao
složen i kompleksan proces koji uključuje ekonomske, socijalne pa
i klimatske promene kao posledicu tehnološkog razvoja čovečanstva, može da utiče i na ustaljene karakteristike i distribuciju egzotičnih
zaraznih bolesti i zoonoza. Očigledne klimatske promene, kao i širenje areala za vektore zaraznih bolesti u žižu interesovanja stavile su,
ne samo bolest plavog jezika koja je već 15 godina prisutna u skoro
svim evropskim zemljama, već i nodularni (Lumpy) dermatitis koji je u
avgustu 2015. godine po prvi put registrovan u Grčkoj. Uz afričku kugu svinja, lista bolesti koje potencijalno ugrožavaju evropski kontinet
se neprekidno širi, a u sklopu ovog fenomena, svakako treba pomenuti i izdvojene slučajeve pojave davno iskorenjenih bolesti, kao što je
izolovan slučaj sakagije konja u Nemačkoj, što epizootiološku situaciju u Evropi čini posebno komplikovanom.
AB  - INFECTUOUS DISEASES GLOBALIZATION TRENDS –
CURRENT EPIZOOTIOLOGICAL SITUATION IN EUROPE
Last years of the previous century have shown a trend of expansion of exotic infectious diseases which, up to then, did not show a significant epizootiological and epidemiological potential. The first surprise was brought up by the unexpected West Nile fever,
previously considered as a local disease with a small potential for spreading. Still, West
Nile fever has spread from Africa to America and thereon all over the world in less than
10 years. In the first decade of the 21st century it has become clear that globalization is a
complex process which includes economic, social and climatic changes due to the fact that
technological development can affect the established features and distribution of exotic infectious diseases and zoonoses. Obvious climate changes, as well as the enlarging of the
areal for infectious diseases vectors have set a spotlight not only on Blue tongue disease
which, has been present in Europe for over 15 years, but also on nodular lumpy dermatitis
which has been registered in Greece for the first time in August 2015. Along with African
swine fever, the list of diseases which threaten the European continent is in constant expansion. In the framework of this phenomenon, occasional case reports of diseases considered to be eradicated a long time ago, such as a case of glanders in Germany must be
taken into account, thus making the epizootiological situation in Europe even more complex.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
T2  - XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA
T1  - Trend globalizacije zaraznih bolesti – aktuelna epizootiološka situacija u Evropi
SP  - 3
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2631
ER  - 

@misc{
author = "Radojičić, Sonja and Valčić, Miroslav and Stević, Nataša",
year = "2016",
abstract = "Poslednje godine prošlog veka ukazale su na trend širenja egzotičnih zaraznih bolesti koje do tada nisu imale bitan epizootiološki i
epidemiološki potencijal. Prva iznenađenja donela je groznica Zapadnog Nila, naizgled lokalna bolest malog potencijala širenja, koja se
za nepunih 10 godina iz Afrike prvo proširila na Ameriku a zatim i ceo
svet. U prvoj deceniji 21. veka, postalo je jasno da globalizacija kao
složen i kompleksan proces koji uključuje ekonomske, socijalne pa
i klimatske promene kao posledicu tehnološkog razvoja čovečanstva, može da utiče i na ustaljene karakteristike i distribuciju egzotičnih
zaraznih bolesti i zoonoza. Očigledne klimatske promene, kao i širenje areala za vektore zaraznih bolesti u žižu interesovanja stavile su,
ne samo bolest plavog jezika koja je već 15 godina prisutna u skoro
svim evropskim zemljama, već i nodularni (Lumpy) dermatitis koji je u
avgustu 2015. godine po prvi put registrovan u Grčkoj. Uz afričku kugu svinja, lista bolesti koje potencijalno ugrožavaju evropski kontinet
se neprekidno širi, a u sklopu ovog fenomena, svakako treba pomenuti i izdvojene slučajeve pojave davno iskorenjenih bolesti, kao što je
izolovan slučaj sakagije konja u Nemačkoj, što epizootiološku situaciju u Evropi čini posebno komplikovanom., INFECTUOUS DISEASES GLOBALIZATION TRENDS –
CURRENT EPIZOOTIOLOGICAL SITUATION IN EUROPE
Last years of the previous century have shown a trend of expansion of exotic infectious diseases which, up to then, did not show a significant epizootiological and epidemiological potential. The first surprise was brought up by the unexpected West Nile fever,
previously considered as a local disease with a small potential for spreading. Still, West
Nile fever has spread from Africa to America and thereon all over the world in less than
10 years. In the first decade of the 21st century it has become clear that globalization is a
complex process which includes economic, social and climatic changes due to the fact that
technological development can affect the established features and distribution of exotic infectious diseases and zoonoses. Obvious climate changes, as well as the enlarging of the
areal for infectious diseases vectors have set a spotlight not only on Blue tongue disease
which, has been present in Europe for over 15 years, but also on nodular lumpy dermatitis
which has been registered in Greece for the first time in August 2015. Along with African
swine fever, the list of diseases which threaten the European continent is in constant expansion. In the framework of this phenomenon, occasional case reports of diseases considered to be eradicated a long time ago, such as a case of glanders in Germany must be
taken into account, thus making the epizootiological situation in Europe even more complex.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA",
title = "Trend globalizacije zaraznih bolesti – aktuelna epizootiološka situacija u Evropi",
pages = "3-12",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2631"
}

Radojičić, S., Valčić, M.,& Stević, N.. (2016). Trend globalizacije zaraznih bolesti – aktuelna epizootiološka situacija u Evropi. in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_2631

Radojičić S, Valčić M, Stević N. Trend globalizacije zaraznih bolesti – aktuelna epizootiološka situacija u Evropi. in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA. 2016;:3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_2631 .

Radojičić, Sonja, Valčić, Miroslav, Stević, Nataša, "Trend globalizacije zaraznih bolesti – aktuelna epizootiološka situacija u Evropi" in XXXVIII SEMINAR ZA INOVACIJE ZNANJA VETERINARA (2016):3-12,
https://hdl.handle.net/21.15107/rcub_veterinar_2631 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Ivović, V.
AU  - Radosavljević, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1400
AB  - Background: Aujeszkys disease is a viral disease of suids caused by Suid Herpesvirus 1. The disease has worldwide distribution with significant economic impact. In Serbia, there is neither an Aujeszkys disease eradication nor national vaccination programme of domestic pigs. Since clinical symptoms of Aujeszkys disease are not specific, it is important to establish a link between clinical signs and presence of ADV active infection in wild boars. The aim of this study was to investigate the possibility of active infection within wild boar showing signs of ADV and also to examine relationship between isolates from domestic pigs and wild boar. Having in mind that virus has not been previously isolated from wild boars in Serbia, we report the first isolation of Suid Herpesvirus 1 from this species in Serbia. Results: Tissue and serum samples from 40 wild boars from eastern Serbia were examined for evidence of Aujeszkys disease (AD). Suid Herpesvirus 1 (SHV1), the cause of AD was isolated on PK15 cell line from three tissue samples, inducing cytopathic effect (CPE) with syncytia forming, and viral genome was detected by polymerase chain reaction (PCR) in eight samples. Genetic analysis of us4, us9 and ul49.5 partial sequences showed high homology between ADV isolates from wild boars and between isolates from wild boars and domestic animals. Neutralizing antibodies were not detected by virus neutralisation test (VNT) in sera from four out of eight PCR positive wild boars suggesting recent infection in those animals. Conclusions: This is the first demonstration of Aujeszkys disease virus (ADV) in the wild boar population in Serbia although seroconversion has been detected previously.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - Evidence of Aujeszky s disease in wild boar in Serbia
VL  - 12
SP  - 134
DO  - 10.1186/s12917-016-0758-9
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Ivović, V. and Radosavljević, Vladimir",
year = "2016",
abstract = "Background: Aujeszkys disease is a viral disease of suids caused by Suid Herpesvirus 1. The disease has worldwide distribution with significant economic impact. In Serbia, there is neither an Aujeszkys disease eradication nor national vaccination programme of domestic pigs. Since clinical symptoms of Aujeszkys disease are not specific, it is important to establish a link between clinical signs and presence of ADV active infection in wild boars. The aim of this study was to investigate the possibility of active infection within wild boar showing signs of ADV and also to examine relationship between isolates from domestic pigs and wild boar. Having in mind that virus has not been previously isolated from wild boars in Serbia, we report the first isolation of Suid Herpesvirus 1 from this species in Serbia. Results: Tissue and serum samples from 40 wild boars from eastern Serbia were examined for evidence of Aujeszkys disease (AD). Suid Herpesvirus 1 (SHV1), the cause of AD was isolated on PK15 cell line from three tissue samples, inducing cytopathic effect (CPE) with syncytia forming, and viral genome was detected by polymerase chain reaction (PCR) in eight samples. Genetic analysis of us4, us9 and ul49.5 partial sequences showed high homology between ADV isolates from wild boars and between isolates from wild boars and domestic animals. Neutralizing antibodies were not detected by virus neutralisation test (VNT) in sera from four out of eight PCR positive wild boars suggesting recent infection in those animals. Conclusions: This is the first demonstration of Aujeszkys disease virus (ADV) in the wild boar population in Serbia although seroconversion has been detected previously.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "Evidence of Aujeszky s disease in wild boar in Serbia",
volume = "12",
pages = "134",
doi = "10.1186/s12917-016-0758-9"
}

Milićević, V., Radojičić, S., Valčić, M., Ivović, V.,& Radosavljević, V.. (2016). Evidence of Aujeszky s disease in wild boar in Serbia. in BMC Veterinary Research
BMC, London., 12, 134.
https://doi.org/10.1186/s12917-016-0758-9

Milićević V, Radojičić S, Valčić M, Ivović V, Radosavljević V. Evidence of Aujeszky s disease in wild boar in Serbia. in BMC Veterinary Research. 2016;12:134.
doi:10.1186/s12917-016-0758-9 .

Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Ivović, V., Radosavljević, Vladimir, "Evidence of Aujeszky s disease in wild boar in Serbia" in BMC Veterinary Research, 12 (2016):134,
https://doi.org/10.1186/s12917-016-0758-9 . .

TY  - JOUR
AU  - Obrenović, Sonja
AU  - Ristanović, Elizabeta
AU  - Cekanac, Radovan
AU  - Radulović, Željko
AU  - Ilić, Vesna
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1179
AB  - In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia
VL  - 65
IS  - 1
SP  - 99
EP  - 110
DO  - 10.1515/acve-2015-0008
ER  - 

@article{
author = "Obrenović, Sonja and Ristanović, Elizabeta and Cekanac, Radovan and Radulović, Željko and Ilić, Vesna",
year = "2015",
abstract = "In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia",
volume = "65",
number = "1",
pages = "99-110",
doi = "10.1515/acve-2015-0008"
}

Obrenović, S., Ristanović, E., Cekanac, R., Radulović, Ž.,& Ilić, V.. (2015). Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 99-110.
https://doi.org/10.1515/acve-2015-0008

Obrenović S, Ristanović E, Cekanac R, Radulović Ž, Ilić V. Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia. in Acta Veterinaria-Beograd. 2015;65(1):99-110.
doi:10.1515/acve-2015-0008 .

Obrenović, Sonja, Ristanović, Elizabeta, Cekanac, Radovan, Radulović, Željko, Ilić, Vesna, "Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia" in Acta Veterinaria-Beograd, 65, no. 1 (2015):99-110,
https://doi.org/10.1515/acve-2015-0008 . .