TY  - JOUR
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Vejnović, Branislav
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2942
AB  - Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.
PB  - MDPI
T2  - Insects
T1  - Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay
VL  - 14
IS  - 5
SP  - 451
DO  - 10.3390/insects14050451
ER  - 

@article{
author = "Glavinić, Uroš and Rajković, Milan and Ristanić, Marko and Stevanović, Jevrosima and Vejnović, Branislav and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.",
publisher = "MDPI",
journal = "Insects",
title = "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay",
volume = "14",
number = "5",
pages = "451",
doi = "10.3390/insects14050451"
}

Glavinić, U., Rajković, M., Ristanić, M., Stevanović, J., Vejnović, B., Đelić, N.,& Stanimirović, Z.. (2023). Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects
MDPI., 14(5), 451.
https://doi.org/10.3390/insects14050451

Glavinić U, Rajković M, Ristanić M, Stevanović J, Vejnović B, Đelić N, Stanimirović Z. Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects. 2023;14(5):451.
doi:10.3390/insects14050451 .

Glavinić, Uroš, Rajković, Milan, Ristanić, Marko, Stevanović, Jevrosima, Vejnović, Branislav, Đelić, Ninoslav, Stanimirović, Zoran, "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay" in Insects, 14, no. 5 (2023):451,
https://doi.org/10.3390/insects14050451 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Bogunović, Danica
AU  - Vejnović, Branislav
AU  - Davitkov, Darko
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3191
AB  - Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various
molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim
of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of
sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole
blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure
and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote,
Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used
in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating
microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA
damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe
clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all
groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that
leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the
modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from
the dogs’ blood.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis
VL  - 322
SP  - 110008
DO  - 10.1016/j.vetpar.2023.110008
ER  - 

@article{
author = "Rajković, Milan and Glavinić, Uroš and Bogunović, Danica and Vejnović, Branislav and Davitkov, Darko and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various
molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim
of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of
sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole
blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure
and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote,
Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used
in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating
microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA
damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe
clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all
groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that
leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the
modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from
the dogs’ blood.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis",
volume = "322",
pages = "110008",
doi = "10.1016/j.vetpar.2023.110008"
}

Rajković, M., Glavinić, U., Bogunović, D., Vejnović, B., Davitkov, D., Đelić, N.,& Stanimirović, Z.. (2023). “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology
Elsevier., 322, 110008.
https://doi.org/10.1016/j.vetpar.2023.110008

Rajković M, Glavinić U, Bogunović D, Vejnović B, Davitkov D, Đelić N, Stanimirović Z. “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology. 2023;322:110008.
doi:10.1016/j.vetpar.2023.110008 .

Rajković, Milan, Glavinić, Uroš, Bogunović, Danica, Vejnović, Branislav, Davitkov, Darko, Đelić, Ninoslav, Stanimirović, Zoran, "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis" in Veterinary Parasitology, 322 (2023):110008,
https://doi.org/10.1016/j.vetpar.2023.110008 . .

TY  - CONF
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Glavinić, Uroš
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3141
AB  - Uslov opstanka agroekosistema je postojanje raznolikosti živog sveta u njima, a
za očuvanje biodiverziteta neophodna je procena, verifikacija i sistematizacija
razlika na svim nivoima: genetičkom, specijskom i ekosistemskom. U ovom radu
prikazane su analize DNK i savremeni biotehnološki alati koji obezbeđuju procenu genetičkog diverziteta u agroekosistemima i posredno doprinose očuvanju
raznolikosti vrsta i ekosistema. Genomika, zbog opadanja cene sekvenciranja,
pruža sve više mogućnosti da se sa povećanom preciznošću obezbede odgovori
na genetička pitanja na koja se prethodno odgovaralo sa nekolicinom molekularnih markera. Metabarkodiranje obećava brže, jeftinije i tačnije procene
biodiverziteta. Podaci iz genomike i transkriptomike mogu da se upotrebe radi
procene potencijala populacija da se adaptiraju na nove izazove. Konačno, navedeni su problemi koji ukazuju na potrebu daljih usavršavanja postojećih
metoda genomike i transkriptomike u funkciji procene i očuvanja biodiverziteta.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Analize dnk u proceni biodiverziteta u agroekosistemima
SP  - 98
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3141
ER  - 

@conference{
author = "Stevanović, Jevrosima and Ristanić, Marko and Glavinić, Uroš and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Uslov opstanka agroekosistema je postojanje raznolikosti živog sveta u njima, a
za očuvanje biodiverziteta neophodna je procena, verifikacija i sistematizacija
razlika na svim nivoima: genetičkom, specijskom i ekosistemskom. U ovom radu
prikazane su analize DNK i savremeni biotehnološki alati koji obezbeđuju procenu genetičkog diverziteta u agroekosistemima i posredno doprinose očuvanju
raznolikosti vrsta i ekosistema. Genomika, zbog opadanja cene sekvenciranja,
pruža sve više mogućnosti da se sa povećanom preciznošću obezbede odgovori
na genetička pitanja na koja se prethodno odgovaralo sa nekolicinom molekularnih markera. Metabarkodiranje obećava brže, jeftinije i tačnije procene
biodiverziteta. Podaci iz genomike i transkriptomike mogu da se upotrebe radi
procene potencijala populacija da se adaptiraju na nove izazove. Konačno, navedeni su problemi koji ukazuju na potrebu daljih usavršavanja postojećih
metoda genomike i transkriptomike u funkciji procene i očuvanja biodiverziteta.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Analize dnk u proceni biodiverziteta u agroekosistemima",
pages = "98-108",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3141"
}

Stevanović, J., Ristanić, M., Glavinić, U., Đelić, N.,& Stanimirović, Z.. (2023). Analize dnk u proceni biodiverziteta u agroekosistemima. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 98-108.
https://hdl.handle.net/21.15107/rcub_veterinar_3141

Stevanović J, Ristanić M, Glavinić U, Đelić N, Stanimirović Z. Analize dnk u proceni biodiverziteta u agroekosistemima. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:98-108.
https://hdl.handle.net/21.15107/rcub_veterinar_3141 .

Stevanović, Jevrosima, Ristanić, Marko, Glavinić, Uroš, Đelić, Ninoslav, Stanimirović, Zoran, "Analize dnk u proceni biodiverziteta u agroekosistemima" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):98-108,
https://hdl.handle.net/21.15107/rcub_veterinar_3141 .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Borozan, Sunčica
AU  - Dimitrijević-Srećković, Vesna
AU  - Pajović, Nevena
AU  - Mirilović, Milorad
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2811
AB  - Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T3) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T3 regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro
VL  - 23
VL  - 16
SP  - 9072
DO  - 10.3390/ijms23169072
ER  - 

@article{
author = "Đelić, Ninoslav and Borozan, Sunčica and Dimitrijević-Srećković, Vesna and Pajović, Nevena and Mirilović, Milorad and Stopper, Helga and Stanimirović, Zoran",
year = "2022",
abstract = "Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T3) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T3 regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro",
volume = "23, 16",
pages = "9072",
doi = "10.3390/ijms23169072"
}

Đelić, N., Borozan, S., Dimitrijević-Srećković, V., Pajović, N., Mirilović, M., Stopper, H.,& Stanimirović, Z.. (2022). Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro. in International Journal of Molecular Sciences
MDPI., 23, 9072.
https://doi.org/10.3390/ijms23169072

Đelić N, Borozan S, Dimitrijević-Srećković V, Pajović N, Mirilović M, Stopper H, Stanimirović Z. Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro. in International Journal of Molecular Sciences. 2022;23:9072.
doi:10.3390/ijms23169072 .

Đelić, Ninoslav, Borozan, Sunčica, Dimitrijević-Srećković, Vesna, Pajović, Nevena, Mirilović, Milorad, Stopper, Helga, Stanimirović, Zoran, "Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro" in International Journal of Molecular Sciences, 23 (2022):9072,
https://doi.org/10.3390/ijms23169072 . .

TY  - JOUR
AU  - Marković, Aleksandra
AU  - Tubić Vukajlović, Jovana
AU  - Grujičić, Darko
AU  - Radović Jakovljević, Marina
AU  - Stanković, Milan
AU  - Djordjević, Katarina
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Milošević-Djordjević, Olivera
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2244
AB  - The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.
AB  - The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.
PB  - Taylor and Francis
T2  - Drug and Chemical Toxicology
T1  - Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro
VL  - 45
IS  - 2
SP  - 940
EP  - 945
DO  - 10.1080/01480545.2020.1802477
ER  - 

@article{
author = "Marković, Aleksandra and Tubić Vukajlović, Jovana and Grujičić, Darko and Radović Jakovljević, Marina and Stanković, Milan and Djordjević, Katarina and Đelić, Ninoslav and Radaković, Milena and Milošević-Djordjević, Olivera",
year = "2022",
abstract = "The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment., The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.",
publisher = "Taylor and Francis",
journal = "Drug and Chemical Toxicology",
title = "Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro",
volume = "45",
number = "2",
pages = "940-945",
doi = "10.1080/01480545.2020.1802477"
}

Marković, A., Tubić Vukajlović, J., Grujičić, D., Radović Jakovljević, M., Stanković, M., Djordjević, K., Đelić, N., Radaković, M.,& Milošević-Djordjević, O.. (2022). Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro. in Drug and Chemical Toxicology
Taylor and Francis., 45(2), 940-945.
https://doi.org/10.1080/01480545.2020.1802477

Marković A, Tubić Vukajlović J, Grujičić D, Radović Jakovljević M, Stanković M, Djordjević K, Đelić N, Radaković M, Milošević-Djordjević O. Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro. in Drug and Chemical Toxicology. 2022;45(2):940-945.
doi:10.1080/01480545.2020.1802477 .

Marković, Aleksandra, Tubić Vukajlović, Jovana, Grujičić, Darko, Radović Jakovljević, Marina, Stanković, Milan, Djordjević, Katarina, Đelić, Ninoslav, Radaković, Milena, Milošević-Djordjević, Olivera, "Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro" in Drug and Chemical Toxicology, 45, no. 2 (2022):940-945,
https://doi.org/10.1080/01480545.2020.1802477 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Ćosić, Milivoje
AU  - Dimitrijević-Srećković, Vesna
AU  - Ilić, Iva
AU  - Đelić, Ninoslav
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2239
AB  - Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type
AB  - Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.
PB  - Faculty of Veterinary Medicine, Belgrade University
T2  - Acta Veterinaria
T2  - Acta Veterinaria-Beograd
T1  - Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?
T1  - Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?
VL  - 71
IS  - 3
SP  - 273
EP  - 284
DO  - 10.2478/acve-2021-0024
ER  - 

@article{
author = "Rajković, Milan and Glavinić, Uroš and Ristanić, Marko and Ćosić, Milivoje and Dimitrijević-Srećković, Vesna and Ilić, Iva and Đelić, Ninoslav",
year = "2021",
abstract = "Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type, Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.",
publisher = "Faculty of Veterinary Medicine, Belgrade University",
journal = "Acta Veterinaria, Acta Veterinaria-Beograd",
title = "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?, Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?",
volume = "71",
number = "3",
pages = "273-284",
doi = "10.2478/acve-2021-0024"
}

Rajković, M., Glavinić, U., Ristanić, M., Ćosić, M., Dimitrijević-Srećković, V., Ilić, I.,& Đelić, N.. (2021). Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria
Faculty of Veterinary Medicine, Belgrade University., 71(3), 273-284.
https://doi.org/10.2478/acve-2021-0024

Rajković M, Glavinić U, Ristanić M, Ćosić M, Dimitrijević-Srećković V, Ilić I, Đelić N. Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria. 2021;71(3):273-284.
doi:10.2478/acve-2021-0024 .

Rajković, Milan, Glavinić, Uroš, Ristanić, Marko, Ćosić, Milivoje, Dimitrijević-Srećković, Vesna, Ilić, Iva, Đelić, Ninoslav, "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?" in Acta Veterinaria, 71, no. 3 (2021):273-284,
https://doi.org/10.2478/acve-2021-0024 . .

TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2020
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier
T2  - Mutation Research/Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
SP  - 108371
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier",
journal = "Mutation Research/Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
pages = "108371",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research
Elsevier., 787, 108371.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research. 2021;787:108371.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research/Reviews in Mutation Research, 787 (2021):108371,
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Tubić Vukajlović, Jovana
AU  - Kosanić, Marijana
AU  - Ranković, Branislav
AU  - Stanojković, Tatjana
AU  - Marković, Aleksandra
AU  - Grujičić, Darko
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Milošević-Djordjević, Olivera
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2318
AB  - Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity.
AB  - Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.
PB  - Societatea de Științe Farmaceutice din România
T2  - Farmacia
T1  - Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum
VL  - 69
IS  - 5
SP  - 974
EP  - 979
DO  - 10.31925/FARMACIA.2021.5.22
ER  - 

@article{
author = "Tubić Vukajlović, Jovana and Kosanić, Marijana and Ranković, Branislav and Stanojković, Tatjana and Marković, Aleksandra and Grujičić, Darko and Đelić, Ninoslav and Radaković, Milena and Milošević-Djordjević, Olivera",
year = "2021",
abstract = "Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity., Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.",
publisher = "Societatea de Științe Farmaceutice din România",
journal = "Farmacia",
title = "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum",
volume = "69",
number = "5",
pages = "974-979",
doi = "10.31925/FARMACIA.2021.5.22"
}

Tubić Vukajlović, J., Kosanić, M., Ranković, B., Stanojković, T., Marković, A., Grujičić, D., Đelić, N., Radaković, M.,& Milošević-Djordjević, O.. (2021). Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia
Societatea de Științe Farmaceutice din România., 69(5), 974-979.
https://doi.org/10.31925/FARMACIA.2021.5.22

Tubić Vukajlović J, Kosanić M, Ranković B, Stanojković T, Marković A, Grujičić D, Đelić N, Radaković M, Milošević-Djordjević O. Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia. 2021;69(5):974-979.
doi:10.31925/FARMACIA.2021.5.22 .

Tubić Vukajlović, Jovana, Kosanić, Marijana, Ranković, Branislav, Stanojković, Tatjana, Marković, Aleksandra, Grujičić, Darko, Đelić, Ninoslav, Radaković, Milena, Milošević-Djordjević, Olivera, "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum" in Farmacia, 69, no. 5 (2021):974-979,
https://doi.org/10.31925/FARMACIA.2021.5.22 . .

TY  - JOUR
AU  - Nešić, Ivana
AU  - Krstić, Nikola
AU  - Đelić, Ninoslav
AU  - Zdravković, Marija
AU  - Tošković, Borislav
AU  - Đorđević, Milena
AU  - Blagojević, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2391
AB  - Background: There are many studies on the morphology of the liver and its blood vessels in experimental animals, but such studies are lacking in the mole rat (Spalax leucodon). The aim of this paper was a detailed basic study on the topography, morphology, vascular and biliary branching systems of the liver in the mole rat. Materials and methods: Coloured gelatine and mixture of coloured lead oxide and linseed oil were injection contrast masses used to obtain vascular and biliary branching pattern in the liver. It was revealed that the liver of the mole rat had five lobes (left, quadrate, right medial, right lateral and caudate lobes). Results: The left, undivided lobe was the largest lobe of the liver. The quadrate lobe was divided into two components by a deep notch. The gallbladder, of cylindrical shape, was present and attached to the quadrate lobe. The common bile duct was formed by the union of the left and right hepatic ducts. The pancreatic duct joined the common bile duct before it entered the duodenum. In the present study, only the right medial lobe and quadrate lobe always showed a single lobar artery, portal and hepatic veins. The left lobe showed four lobar arteries, portal and hepatic veins. The caudate lobe with its two processes and the right lateral and medial lobes had different arterial and portal blood supply as well as hepatic and biliary drainage of these lobes. The intrahepatic branches of the proper hepatic artery ran parallel to the branches of the common portal vein in the same lobes of the liver. Conclusions: The results of this study are significant for comparative studies among different species of rodents and other experimental animals. Morphology, vasculature and biliary tract of the liver in the mole rat were similar to that of other experimental animals and identified differences may be related to the adaptation to the mode of life and diet of this rodent.
PB  - Via Medica
T2  - Folia Morphologica (Poland)
T1  - Liver anatomy, intrahepatic vascular and biliary branching system of the mole rat (Spalax leucodon)
VL  - 80
IS  - 4
SP  - 888
EP  - 894
DO  - 10.5603/FM.A2020.0132
ER  - 

@article{
author = "Nešić, Ivana and Krstić, Nikola and Đelić, Ninoslav and Zdravković, Marija and Tošković, Borislav and Đorđević, Milena and Blagojević, Miloš",
year = "2021",
abstract = "Background: There are many studies on the morphology of the liver and its blood vessels in experimental animals, but such studies are lacking in the mole rat (Spalax leucodon). The aim of this paper was a detailed basic study on the topography, morphology, vascular and biliary branching systems of the liver in the mole rat. Materials and methods: Coloured gelatine and mixture of coloured lead oxide and linseed oil were injection contrast masses used to obtain vascular and biliary branching pattern in the liver. It was revealed that the liver of the mole rat had five lobes (left, quadrate, right medial, right lateral and caudate lobes). Results: The left, undivided lobe was the largest lobe of the liver. The quadrate lobe was divided into two components by a deep notch. The gallbladder, of cylindrical shape, was present and attached to the quadrate lobe. The common bile duct was formed by the union of the left and right hepatic ducts. The pancreatic duct joined the common bile duct before it entered the duodenum. In the present study, only the right medial lobe and quadrate lobe always showed a single lobar artery, portal and hepatic veins. The left lobe showed four lobar arteries, portal and hepatic veins. The caudate lobe with its two processes and the right lateral and medial lobes had different arterial and portal blood supply as well as hepatic and biliary drainage of these lobes. The intrahepatic branches of the proper hepatic artery ran parallel to the branches of the common portal vein in the same lobes of the liver. Conclusions: The results of this study are significant for comparative studies among different species of rodents and other experimental animals. Morphology, vasculature and biliary tract of the liver in the mole rat were similar to that of other experimental animals and identified differences may be related to the adaptation to the mode of life and diet of this rodent.",
publisher = "Via Medica",
journal = "Folia Morphologica (Poland)",
title = "Liver anatomy, intrahepatic vascular and biliary branching system of the mole rat (Spalax leucodon)",
volume = "80",
number = "4",
pages = "888-894",
doi = "10.5603/FM.A2020.0132"
}

Nešić, I., Krstić, N., Đelić, N., Zdravković, M., Tošković, B., Đorđević, M.,& Blagojević, M.. (2021). Liver anatomy, intrahepatic vascular and biliary branching system of the mole rat (Spalax leucodon). in Folia Morphologica (Poland)
Via Medica., 80(4), 888-894.
https://doi.org/10.5603/FM.A2020.0132

Nešić I, Krstić N, Đelić N, Zdravković M, Tošković B, Đorđević M, Blagojević M. Liver anatomy, intrahepatic vascular and biliary branching system of the mole rat (Spalax leucodon). in Folia Morphologica (Poland). 2021;80(4):888-894.
doi:10.5603/FM.A2020.0132 .

Nešić, Ivana, Krstić, Nikola, Đelić, Ninoslav, Zdravković, Marija, Tošković, Borislav, Đorđević, Milena, Blagojević, Miloš, "Liver anatomy, intrahepatic vascular and biliary branching system of the mole rat (Spalax leucodon)" in Folia Morphologica (Poland), 80, no. 4 (2021):888-894,
https://doi.org/10.5603/FM.A2020.0132 . .

TY  - JOUR
AU  - Sjakste, Nikolajs
AU  - Đelić, Ninoslav
AU  - Dzintare, Maija
AU  - Živković, Lada
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1817
AB  - The review summarizes literature data on the DNA-binding, DNA-protecting and DNA-damaging activities of a range of natural human endogenous and exogenous compounds. Small natural organic molecules bind DNA in a site-specific mode, by arranging tight touch with the structure of the major and minor grooves, as well as individual bases in the local duplex DNA. Polyphenols are the best-studied exogenous compounds from this point of view. Many of them demonstrate hormetic effects, producing both beneficial and damaging effects. An attempt to establish the dependence of DNA damage or DNA protection on the concentration of the compound turned out to be successful for some polyphenols, daidzein, genistein and resveratrol, which were DNA protecting in low concentrations and DNA damaging in high concentrations. There was no evident dependence on concentration for quercetin and kaempferol. Probably, the DNA-protecting effect is associated with the affinity to DNA. Caffeine and theophylline are DNA binders; at the same time, they favor DNA repair. Although most alkaloids damage DNA, berberine can protect DNA against damage. Among the endogenous compounds, hormones belonging to the amine class, thyroid and steroid hormones appear to bind DNA and produce some DNA damage. Thus, natural compounds continue to reveal beneficial or adverse effects on genome integrity and provide a promising source of therapeutic activities.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts
VL  - 323
SP  - 109030
DO  - 10.1016/j.cbi.2020.109030
ER  - 

@article{
author = "Sjakste, Nikolajs and Đelić, Ninoslav and Dzintare, Maija and Živković, Lada",
year = "2020",
abstract = "The review summarizes literature data on the DNA-binding, DNA-protecting and DNA-damaging activities of a range of natural human endogenous and exogenous compounds. Small natural organic molecules bind DNA in a site-specific mode, by arranging tight touch with the structure of the major and minor grooves, as well as individual bases in the local duplex DNA. Polyphenols are the best-studied exogenous compounds from this point of view. Many of them demonstrate hormetic effects, producing both beneficial and damaging effects. An attempt to establish the dependence of DNA damage or DNA protection on the concentration of the compound turned out to be successful for some polyphenols, daidzein, genistein and resveratrol, which were DNA protecting in low concentrations and DNA damaging in high concentrations. There was no evident dependence on concentration for quercetin and kaempferol. Probably, the DNA-protecting effect is associated with the affinity to DNA. Caffeine and theophylline are DNA binders; at the same time, they favor DNA repair. Although most alkaloids damage DNA, berberine can protect DNA against damage. Among the endogenous compounds, hormones belonging to the amine class, thyroid and steroid hormones appear to bind DNA and produce some DNA damage. Thus, natural compounds continue to reveal beneficial or adverse effects on genome integrity and provide a promising source of therapeutic activities.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts",
volume = "323",
pages = "109030",
doi = "10.1016/j.cbi.2020.109030"
}

Sjakste, N., Đelić, N., Dzintare, M.,& Živković, L.. (2020). DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 323, 109030.
https://doi.org/10.1016/j.cbi.2020.109030

Sjakste N, Đelić N, Dzintare M, Živković L. DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts. in Chemico-Biological Interactions. 2020;323:109030.
doi:10.1016/j.cbi.2020.109030 .

Sjakste, Nikolajs, Đelić, Ninoslav, Dzintare, Maija, Živković, Lada, "DNA-binding and DNA-protecting activities of small natural organic molecules and food extracts" in Chemico-Biological Interactions, 323 (2020):109030,
https://doi.org/10.1016/j.cbi.2020.109030 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2050
AB  - Objective. Inosine 5'-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5’-monofosfat dehidrogenaze
(IMPDH) povećana je u ćelijama karcinoma. Tiazofurin
selektivno inhibira aktivnost IMPDH i odobren je za lečenje
različitih karcinoma i novih virusnih bolesti. Njegova široko
rasprostranjena upotreba bila je ograničena jer je utvrđeno
da je izloženost tiazofurinu pod određenim okolnostima
imala veću incidencu ozbiljne nehematološke toksičnosti.
Stoga je cilj ove studije bio da se pomoću komet testa ispita
genotoksično delovanje i izazivanje DNK oštećenja
tiazofurinom.
Metode. Sposobnost tiazofurina da izazove DNK
oštećenje procenjena je primenom elektroforeze DNK
pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su
bile izložene trima konačnim koncentracijama tiazofurina (1
µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro.
Rezultati. Naši rezultati ukazuju na to da je tiazofurin
proizveo značajan nivo DNK oštećenja na ćelijama pune krvi
nakon 30 minuta izlaganja u odnosu na kontrolu. Sve
ispitivane koncentracije su dovele do značajnog nastanka
kometa, pri čemu je nivo oštećenja rastao s koncentracijom.
Zaključak. Naše istraživanje ćelija tretiranih
tiazofurinom i njihova reakcija na izazivanje DNK oštećenja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima
PB  - Kragujevac : Srpsko lekarsko društvo - Okružna podružnica Kragujevac
T2  - Medicinski časopis
T1  - Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay
T1  - Analiza dnk oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5'-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5’-monofosfat dehidrogenaze
(IMPDH) povećana je u ćelijama karcinoma. Tiazofurin
selektivno inhibira aktivnost IMPDH i odobren je za lečenje
različitih karcinoma i novih virusnih bolesti. Njegova široko
rasprostranjena upotreba bila je ograničena jer je utvrđeno
da je izloženost tiazofurinu pod određenim okolnostima
imala veću incidencu ozbiljne nehematološke toksičnosti.
Stoga je cilj ove studije bio da se pomoću komet testa ispita
genotoksično delovanje i izazivanje DNK oštećenja
tiazofurinom.
Metode. Sposobnost tiazofurina da izazove DNK
oštećenje procenjena je primenom elektroforeze DNK
pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su
bile izložene trima konačnim koncentracijama tiazofurina (1
µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro.
Rezultati. Naši rezultati ukazuju na to da je tiazofurin
proizveo značajan nivo DNK oštećenja na ćelijama pune krvi
nakon 30 minuta izlaganja u odnosu na kontrolu. Sve
ispitivane koncentracije su dovele do značajnog nastanka
kometa, pri čemu je nivo oštećenja rastao s koncentracijom.
Zaključak. Naše istraživanje ćelija tretiranih
tiazofurinom i njihova reakcija na izazivanje DNK oštećenja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima",
publisher = "Kragujevac : Srpsko lekarsko društvo - Okružna podružnica Kragujevac",
journal = "Medicinski časopis",
title = "Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay, Analiza dnk oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay. in Medicinski časopis
Kragujevac : Srpsko lekarsko društvo - Okružna podružnica Kragujevac., 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798

Topalović D, Živković L, Đelić N, Bajić V, Spremo-Potparević B. Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay. in Medicinski časopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay" in Medicinski časopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Dimirijević-Srećković, Vesna
AU  - Pajović, Nevena
AU  - Vejnović, Branislav
AU  - Borozan, Nevena
AU  - Bankoglu, Ezgi Eylül
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2546
AB  - Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as
the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes.
Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells
(PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to
PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress
(TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of
adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In
summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA
damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis
also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as
significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased
antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results
could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules
which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the
possibility of diabetes complications.
PB  - Elsevier
T2  - Mutation Research: Genetic Toxicology and Environmental Mutagenesis
T1  - Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro
VL  - 843
SP  - 81
EP  - 89
DO  - 10.1016/j.mrgentox.2019.01.013
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Borozan, Sunčica and Dimirijević-Srećković, Vesna and Pajović, Nevena and Vejnović, Branislav and Borozan, Nevena and Bankoglu, Ezgi Eylül and Stopper, Helga and Stanimirović, Zoran",
year = "2019",
abstract = "Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as
the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes.
Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells
(PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to
PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress
(TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of
adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In
summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA
damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis
also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as
significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased
antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results
could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules
which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the
possibility of diabetes complications.",
publisher = "Elsevier",
journal = "Mutation Research: Genetic Toxicology and Environmental Mutagenesis",
title = "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro",
volume = "843",
pages = "81-89",
doi = "10.1016/j.mrgentox.2019.01.013"
}

Đelić, N., Radaković, M., Borozan, S., Dimirijević-Srećković, V., Pajović, N., Vejnović, B., Borozan, N., Bankoglu, E. E., Stopper, H.,& Stanimirović, Z.. (2019). Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis
Elsevier., 843, 81-89.
https://doi.org/10.1016/j.mrgentox.2019.01.013

Đelić N, Radaković M, Borozan S, Dimirijević-Srećković V, Pajović N, Vejnović B, Borozan N, Bankoglu EE, Stopper H, Stanimirović Z. Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis. 2019;843:81-89.
doi:10.1016/j.mrgentox.2019.01.013 .

Đelić, Ninoslav, Radaković, Milena, Borozan, Sunčica, Dimirijević-Srećković, Vesna, Pajović, Nevena, Vejnović, Branislav, Borozan, Nevena, Bankoglu, Ezgi Eylül, Stopper, Helga, Stanimirović, Zoran, "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro" in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 843 (2019):81-89,
https://doi.org/10.1016/j.mrgentox.2019.01.013 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Dimirijević-Srecković, Vesna
AU  - Pajović, Nevena
AU  - Vejnović, Branislav
AU  - Borozan, Nevena
AU  - Bankoglu, Ezgi Eylul
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1780
AB  - Diabetes represents one of the major health concerns, especially In developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 mu M) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 mu M) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant Increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro
VL  - 843
SP  - 81
EP  - 89
DO  - 10.1016/j.mrgentox.2019.01.013
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Borozan, Sunčica and Dimirijević-Srecković, Vesna and Pajović, Nevena and Vejnović, Branislav and Borozan, Nevena and Bankoglu, Ezgi Eylul and Stopper, Helga and Stanimirović, Zoran",
year = "2019",
abstract = "Diabetes represents one of the major health concerns, especially In developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 mu M) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 mu M) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant Increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro",
volume = "843",
pages = "81-89",
doi = "10.1016/j.mrgentox.2019.01.013"
}

Đelić, N., Radaković, M., Borozan, S., Dimirijević-Srecković, V., Pajović, N., Vejnović, B., Borozan, N., Bankoglu, E. E., Stopper, H.,& Stanimirović, Z.. (2019). Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 843, 81-89.
https://doi.org/10.1016/j.mrgentox.2019.01.013

Đelić N, Radaković M, Borozan S, Dimirijević-Srecković V, Pajović N, Vejnović B, Borozan N, Bankoglu EE, Stopper H, Stanimirović Z. Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;843:81-89.
doi:10.1016/j.mrgentox.2019.01.013 .

Đelić, Ninoslav, Radaković, Milena, Borozan, Sunčica, Dimirijević-Srecković, Vesna, Pajović, Nevena, Vejnović, Branislav, Borozan, Nevena, Bankoglu, Ezgi Eylul, Stopper, Helga, Stanimirović, Zoran, "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 843 (2019):81-89,
https://doi.org/10.1016/j.mrgentox.2019.01.013 . .

TY  - JOUR
AU  - Montag, Gracia
AU  - Bankoglu, Ezgi Eylul
AU  - Bolte, Annika
AU  - Hintzsche, Henning
AU  - Đelić, Ninoslav
AU  - Stopper, Helga
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1789
AB  - The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay
VL  - 845
SP  - UNSP 402972
DO  - 10.1016/j.mrgentox.2018.10.004
ER  - 

@article{
author = "Montag, Gracia and Bankoglu, Ezgi Eylul and Bolte, Annika and Hintzsche, Henning and Đelić, Ninoslav and Stopper, Helga",
year = "2019",
abstract = "The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay",
volume = "845",
pages = "UNSP 402972",
doi = "10.1016/j.mrgentox.2018.10.004"
}

Montag, G., Bankoglu, E. E., Bolte, A., Hintzsche, H., Đelić, N.,& Stopper, H.. (2019). Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 845, UNSP 402972.
https://doi.org/10.1016/j.mrgentox.2018.10.004

Montag G, Bankoglu EE, Bolte A, Hintzsche H, Đelić N, Stopper H. Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 402972.
doi:10.1016/j.mrgentox.2018.10.004 .

Montag, Gracia, Bankoglu, Ezgi Eylul, Bolte, Annika, Hintzsche, Henning, Đelić, Ninoslav, Stopper, Helga, "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 402972,
https://doi.org/10.1016/j.mrgentox.2018.10.004 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Saša
AU  - Ćupić Miladinović, Dejana
AU  - Ristanić, Marko
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1576
AB  - This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
VL  - 2018
SP  - 1805354
DO  - 10.1155/2018/1805354
ER  - 

@article{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Saša and Ćupić Miladinović, Dejana and Ristanić, Marko and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2018",
abstract = "This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline",
volume = "2018",
pages = "1805354",
doi = "10.1155/2018/1805354"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Ćupić Miladinović, D., Ristanić, M., Spremo-Potparević, B.,& Stanimirović, Z.. (2018). Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2018, 1805354.
https://doi.org/10.1155/2018/1805354

Radaković M, Borozan S, Đelić N, Ivanović S, Ćupić Miladinović D, Ristanić M, Spremo-Potparević B, Stanimirović Z. Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity. 2018;2018:1805354.
doi:10.1155/2018/1805354 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Saša, Ćupić Miladinović, Dejana, Ristanić, Marko, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline" in Oxidative Medicine and Cellular Longevity, 2018 (2018):1805354,
https://doi.org/10.1155/2018/1805354 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1619
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Spremo-Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Spremo-Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Bošnjak-Neumuller, Jasna
AU  - Rancić, Dragana
AU  - Pecinar, Ilinka
AU  - Đelić, Ninoslav
AU  - Dajic-Stevanović, Zora
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1633
AB  - Micromorphological and anatomical analyses of the indumentum of Nepeta rtanjensis, a Serbian/local endemic species, were performed by means of light and electron microscopy and described in detail. The leaves and stems bear numerous glandular trichomes, both peltate and capitate, as well as non-glandular unbranched trichomes. A basal epidermal cell, a short stalk cell and a large round head of four secretory cells constitute one peltate trichome. Capitate trichomes are short-and long-stalked and can be divided into two types, based on the number of glandular head cells, and further into a few subtypes according to the length and number of cells in stalk. Capitate trichomes of smaller length with one-cellular or with a bicellular head have been previously reported in different Nepeta species; however, according to our best knowledge, no existing study has reported the presence of long capitate trichomes on vegetative organs in any other species of this genus.
PB  - Pakistan Botanical Soc, Karachi
T2  - Pakistan Journal of Botany
T1  - Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (Lamiaceae)
VL  - 50
IS  - 1
SP  - 259
EP  - 269
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4810
ER  - 

@article{
author = "Bošnjak-Neumuller, Jasna and Rancić, Dragana and Pecinar, Ilinka and Đelić, Ninoslav and Dajic-Stevanović, Zora",
year = "2018",
abstract = "Micromorphological and anatomical analyses of the indumentum of Nepeta rtanjensis, a Serbian/local endemic species, were performed by means of light and electron microscopy and described in detail. The leaves and stems bear numerous glandular trichomes, both peltate and capitate, as well as non-glandular unbranched trichomes. A basal epidermal cell, a short stalk cell and a large round head of four secretory cells constitute one peltate trichome. Capitate trichomes are short-and long-stalked and can be divided into two types, based on the number of glandular head cells, and further into a few subtypes according to the length and number of cells in stalk. Capitate trichomes of smaller length with one-cellular or with a bicellular head have been previously reported in different Nepeta species; however, according to our best knowledge, no existing study has reported the presence of long capitate trichomes on vegetative organs in any other species of this genus.",
publisher = "Pakistan Botanical Soc, Karachi",
journal = "Pakistan Journal of Botany",
title = "Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (Lamiaceae)",
volume = "50",
number = "1",
pages = "259-269",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4810"
}

Bošnjak-Neumuller, J., Rancić, D., Pecinar, I., Đelić, N.,& Dajic-Stevanović, Z.. (2018). Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (Lamiaceae). in Pakistan Journal of Botany
Pakistan Botanical Soc, Karachi., 50(1), 259-269.
https://hdl.handle.net/21.15107/rcub_agrospace_4810

Bošnjak-Neumuller J, Rancić D, Pecinar I, Đelić N, Dajic-Stevanović Z. Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (Lamiaceae). in Pakistan Journal of Botany. 2018;50(1):259-269.
https://hdl.handle.net/21.15107/rcub_agrospace_4810 .

Bošnjak-Neumuller, Jasna, Rancić, Dragana, Pecinar, Ilinka, Đelić, Ninoslav, Dajic-Stevanović, Zora, "Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (Lamiaceae)" in Pakistan Journal of Botany, 50, no. 1 (2018):259-269,
https://hdl.handle.net/21.15107/rcub_agrospace_4810 .

TY  - JOUR
AU  - Popović-Bubujuk, Slavica
AU  - Đelić, Ninoslav
AU  - Muster, Adrian
AU  - Kataranovski, Dragan
AU  - Anđelković, Marko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1661
AB  - Heavy metal cadmium (Cd), a well-known environmental hazard, exerts a number of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and Dark August (DA) rat strains, were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight, while the control animals received equal volume of sterile phosphate buffered saline. In this investigation individuals of both sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after treatment. The results showed that that cadmium-cloride (CdCl2) exibits the genotoxic effects causing an increase of the frequency of micronuclei depending on concentration, sex, age and strain.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Influence of cadmium chloride on the frequency of micronuclei in da and AO rats
VL  - 50
IS  - 2
SP  - 705
EP  - 716
DO  - 10.2298/GENSR1802705P
ER  - 

@article{
author = "Popović-Bubujuk, Slavica and Đelić, Ninoslav and Muster, Adrian and Kataranovski, Dragan and Anđelković, Marko",
year = "2018",
abstract = "Heavy metal cadmium (Cd), a well-known environmental hazard, exerts a number of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and Dark August (DA) rat strains, were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight, while the control animals received equal volume of sterile phosphate buffered saline. In this investigation individuals of both sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after treatment. The results showed that that cadmium-cloride (CdCl2) exibits the genotoxic effects causing an increase of the frequency of micronuclei depending on concentration, sex, age and strain.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Influence of cadmium chloride on the frequency of micronuclei in da and AO rats",
volume = "50",
number = "2",
pages = "705-716",
doi = "10.2298/GENSR1802705P"
}

Popović-Bubujuk, S., Đelić, N., Muster, A., Kataranovski, D.,& Anđelković, M.. (2018). Influence of cadmium chloride on the frequency of micronuclei in da and AO rats. in Genetika
Društvo genetičara Srbije, Beograd., 50(2), 705-716.
https://doi.org/10.2298/GENSR1802705P

Popović-Bubujuk S, Đelić N, Muster A, Kataranovski D, Anđelković M. Influence of cadmium chloride on the frequency of micronuclei in da and AO rats. in Genetika. 2018;50(2):705-716.
doi:10.2298/GENSR1802705P .

Popović-Bubujuk, Slavica, Đelić, Ninoslav, Muster, Adrian, Kataranovski, Dragan, Anđelković, Marko, "Influence of cadmium chloride on the frequency of micronuclei in da and AO rats" in Genetika, 50, no. 2 (2018):705-716,
https://doi.org/10.2298/GENSR1802705P . .

TY  - CONF
AU  - Tubić, Jovana
AU  - Marković, Aleksandra
AU  - Ranković, Branislav
AU  - Kosanić, Marijana
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Grujičić, Darko
AU  - Milošević-Đorđević, Olivera
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2610
PB  - Beograd : Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.
T1  - Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2610
ER  - 

@conference{
author = "Tubić, Jovana and Marković, Aleksandra and Ranković, Branislav and Kosanić, Marijana and Đelić, Ninoslav and Radaković, Milena and Grujičić, Darko and Milošević-Đorđević, Olivera",
year = "2018",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.",
title = "Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2610"
}

Tubić, J., Marković, A., Ranković, B., Kosanić, M., Đelić, N., Radaković, M., Grujičić, D.,& Milošević-Đorđević, O.. (2018). Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus. in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.
Beograd : Srpsko biološko društvo., 135-135.
https://hdl.handle.net/21.15107/rcub_veterinar_2610

Tubić J, Marković A, Ranković B, Kosanić M, Đelić N, Radaković M, Grujičić D, Milošević-Đorđević O. Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus. in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.. 2018;:135-135.
https://hdl.handle.net/21.15107/rcub_veterinar_2610 .

Tubić, Jovana, Marković, Aleksandra, Ranković, Branislav, Kosanić, Marijana, Đelić, Ninoslav, Radaković, Milena, Grujičić, Darko, Milošević-Đorđević, Olivera, "Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus" in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018. (2018):135-135,
https://hdl.handle.net/21.15107/rcub_veterinar_2610 .

TY  - JOUR
AU  - Bošnjak-Neumuller, Jasna
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Vukovic-Gacić, Branka
AU  - Dajic-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knežević-Vukčević, Jelena
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1473
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).
PB  - Univ Karachi, Karachi
T2  - Pakistan Journal of Pharmaceutical Sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
VL  - 30
IS  - 2
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumuller, Jasna and Radaković, Milena and Đelić, Ninoslav and Vukovic-Gacić, Branka and Dajic-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knežević-Vukčević, Jelena and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).",
publisher = "Univ Karachi, Karachi",
journal = "Pakistan Journal of Pharmaceutical Sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
volume = "30",
number = "2",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumuller, J., Radaković, M., Đelić, N., Vukovic-Gacić, B., Dajic-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knežević-Vukčević, J., Spremo-Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan Journal of Pharmaceutical Sciences
Univ Karachi, Karachi., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumuller J, Radaković M, Đelić N, Vukovic-Gacić B, Dajic-Stevanović Z, Kolarević S, Misić D, Stanković M, Knežević-Vukčević J, Spremo-Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan Journal of Pharmaceutical Sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumuller, Jasna, Radaković, Milena, Đelić, Ninoslav, Vukovic-Gacić, Branka, Dajic-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knežević-Vukčević, Jelena, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan Journal of Pharmaceutical Sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Bošnjak-Neumuller, Jasna
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Kolarević, Stoimir
AU  - Mitić-Ćulafić, Dragana
AU  - Dajic-Stevanović, Zora
AU  - Vukovic-Gacić, Branka
AU  - Knežević-Vukčević, Jelena
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1548
AB  - There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro
VL  - 49
IS  - 2
SP  - 387
EP  - 397
DO  - 10.2298/GENSR1702387B
ER  - 

@article{
author = "Bošnjak-Neumuller, Jasna and Đelić, Ninoslav and Radaković, Milena and Kolarević, Stoimir and Mitić-Ćulafić, Dragana and Dajic-Stevanović, Zora and Vukovic-Gacić, Branka and Knežević-Vukčević, Jelena and Stanimirović, Zoran",
year = "2017",
abstract = "There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro",
volume = "49",
number = "2",
pages = "387-397",
doi = "10.2298/GENSR1702387B"
}

Bošnjak-Neumuller, J., Đelić, N., Radaković, M., Kolarević, S., Mitić-Ćulafić, D., Dajic-Stevanović, Z., Vukovic-Gacić, B., Knežević-Vukčević, J.,& Stanimirović, Z.. (2017). Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 387-397.
https://doi.org/10.2298/GENSR1702387B

Bošnjak-Neumuller J, Đelić N, Radaković M, Kolarević S, Mitić-Ćulafić D, Dajic-Stevanović Z, Vukovic-Gacić B, Knežević-Vukčević J, Stanimirović Z. Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika. 2017;49(2):387-397.
doi:10.2298/GENSR1702387B .

Bošnjak-Neumuller, Jasna, Đelić, Ninoslav, Radaković, Milena, Kolarević, Stoimir, Mitić-Ćulafić, Dragana, Dajic-Stevanović, Zora, Vukovic-Gacić, Branka, Knežević-Vukčević, Jelena, Stanimirović, Zoran, "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro" in Genetika, 49, no. 2 (2017):387-397,
https://doi.org/10.2298/GENSR1702387B . .

TY  - CONF
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Saša
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2717
AB  - The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.
C3  - 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016
T1  - Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline
IS  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2717
ER  - 

@conference{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Saša and Spremo-Potparević, Biljana and Živković, Lada and Stanimirović, Zoran",
year = "2016",
abstract = "The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.",
journal = "3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016",
title = "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline",
number = "71",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2717"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Spremo-Potparević, B., Živković, L.,& Stanimirović, Z.. (2016). Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016(71).
https://hdl.handle.net/21.15107/rcub_veterinar_2717

Radaković M, Borozan S, Đelić N, Ivanović S, Spremo-Potparević B, Živković L, Stanimirović Z. Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016. 2016;(71):null-71.
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Saša, Spremo-Potparević, Biljana, Živković, Lada, Stanimirović, Zoran, "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline" in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016, no. 71 (2016),
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

TY  - JOUR
AU  - Dacić, Stefan
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Lakić, Nada
AU  - Veselinović, Aleksandar
AU  - Ristanić, Marko
AU  - Anđelković, Marko
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1408
AB  - Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay
VL  - 48
IS  - 2
SP  - 617
EP  - 627
DO  - 10.2298/GENSR1602617D
ER  - 

@article{
author = "Dacić, Stefan and Đelić, Ninoslav and Radaković, Milena and Lakić, Nada and Veselinović, Aleksandar and Ristanić, Marko and Anđelković, Marko",
year = "2016",
abstract = "Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 mu g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, chi(2) test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay",
volume = "48",
number = "2",
pages = "617-627",
doi = "10.2298/GENSR1602617D"
}

Dacić, S., Đelić, N., Radaković, M., Lakić, N., Veselinović, A., Ristanić, M.,& Anđelković, M.. (2016). Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika
Društvo genetičara Srbije, Beograd., 48(2), 617-627.
https://doi.org/10.2298/GENSR1602617D

Dacić S, Đelić N, Radaković M, Lakić N, Veselinović A, Ristanić M, Anđelković M. Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay. in Genetika. 2016;48(2):617-627.
doi:10.2298/GENSR1602617D .

Dacić, Stefan, Đelić, Ninoslav, Radaković, Milena, Lakić, Nada, Veselinović, Aleksandar, Ristanić, Marko, Anđelković, Marko, "Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay" in Genetika, 48, no. 2 (2016):617-627,
https://doi.org/10.2298/GENSR1602617D . .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1429
AB  - Context • Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective • The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design • The research team designed a pilot study. Setting •The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants • Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention • Four concentrations of the CS extract— 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL—were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures • Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A—undamaged cells with no tail (<5% damaged DNA); (2) class B—low-level damage (5%-20%); (3) class C—medium-level damage (20%-40%); (4) class D—high-level damage (40%-95%), and (5) class E—total destruction (>95%). Results • The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-μg/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions • The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
PB  - Innovision Communications, Aliso Viejo
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1429
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan and Đelić, Ninoslav and Spremo-Potparević, Biljana",
year = "2016",
abstract = "Context • Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective • The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design • The research team designed a pilot study. Setting •The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants • Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention • Four concentrations of the CS extract— 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL—were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures • Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A—undamaged cells with no tail (<5% damaged DNA); (2) class B—low-level damage (5%-20%); (3) class C—medium-level damage (20%-40%); (4) class D—high-level damage (40%-95%), and (5) class E—total destruction (>95%). Results • The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-μg/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions • The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
publisher = "Innovision Communications, Aliso Viejo",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1429"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V., Đelić, N.,& Spremo-Potparević, B.. (2016). Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay. in Alternative Therapies in Health and Medicine
Innovision Communications, Aliso Viejo., 22, 24-31.
https://hdl.handle.net/21.15107/rcub_veterinar_1429

Vasiljević J, Živković L, Čabarkapa A, Bajić V, Đelić N, Spremo-Potparević B. Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_veterinar_1429 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan, Đelić, Ninoslav, Spremo-Potparević, Biljana, "Cordyceps sinensis: Genotoxic potential in human peripheral blood cells and antigenotoxic properties against hydrogen peroxide by comet assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_veterinar_1429 .

TY  - JOUR
AU  - Maletić, Jelena
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Kukolj, Vladimir
AU  - Aleksić, Nevenka
AU  - Anđelković, Marko
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1183
AB  - Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
VL  - 47
IS  - 1
SP  - 339
EP  - 348
DO  - 10.2298/GENSR1501339M
ER  - 

@article{
author = "Maletić, Jelena and Đelić, Ninoslav and Radaković, Milena and Maletić, Milan and Lakić, Nada and Kukolj, Vladimir and Aleksić, Nevenka and Anđelković, Marko and Stanimirović, Zoran",
year = "2015",
abstract = "Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro",
volume = "47",
number = "1",
pages = "339-348",
doi = "10.2298/GENSR1501339M"
}

Maletić, J., Đelić, N., Radaković, M., Maletić, M., Lakić, N., Kukolj, V., Aleksić, N., Anđelković, M.,& Stanimirović, Z.. (2015). Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 339-348.
https://doi.org/10.2298/GENSR1501339M

Maletić J, Đelić N, Radaković M, Maletić M, Lakić N, Kukolj V, Aleksić N, Anđelković M, Stanimirović Z. Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika. 2015;47(1):339-348.
doi:10.2298/GENSR1501339M .

Maletić, Jelena, Đelić, Ninoslav, Radaković, Milena, Maletić, Milan, Lakić, Nada, Kukolj, Vladimir, Aleksić, Nevenka, Anđelković, Marko, Stanimirović, Zoran, "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro" in Genetika, 47, no. 1 (2015):339-348,
https://doi.org/10.2298/GENSR1501339M . .