TY  - JOUR
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Simeunović, Predrag
AU  - Borozan, Sunčica
AU  - Nedić, Svetlana
AU  - Bojkovski, Jovan
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2238
AB  - This study investigated the effects of dietary supplementation with chestnut tannins (CNT) on metabolic and antioxidant status of prepartum cows along with their colostrum quality. Pregnant multiparous Holstein cows were paired according to parity and body condition score, and assigned either to a diet supplemented with 20 g/d of commercially available product containing chestnut tannins (CNT, n=20) or to an unsupplemented control diet (CON, n=20) for the last 25±2 d of pregnancy. Serum metabolite, insulin and antioxidant capacity indices were measured in blood samples taken at d 25 and d 5 before expected parturition. Chemical composition and IgG concentration were determined in colostrum samples collected from the first milking postpartum. The addition of CNT led to lower BUN (P=0.02) and consequently higher serum glucose (P=0.02) and insulin (P<0.01) concentrations which were associated with lower circulating NEFA (P<0.01) and BHBA (P<0.01) in CNT group than those of CON. The serum paraoxonase 1 (PON 1) activity and total antioxidant capacity (T-AOC) were higher at-5 d in CNT than in CON (P<0.01, P=0.03; respectively). Close-up CNT improved lactose percentage and IgG concentration (P=0.03, P=0.04; respectively) and tended to improve percentage of protein and SNF (Solid Not Fat) in primary colostrum (P=0.06, respectively), without affecting colostrum fat and total solid (P=0.98, P=0.43; respectively). Supplementation of CNT in the diet during close-up period did not have adverse effects on metabolic profiles prepartum. Instead, this feeding regimen was more beneficial to antioxidant capacity and colostrum quality than feeding the control diet.
PB  - Sciendo
T2  - Annals of Animal Science
T1  - Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices
VL  - 21
IS  - 2
SP  - 609
EP  - 621
DO  - 10.2478/aoas-2020-0077
ER  - 

@article{
author = "Prodanović, Radiša and Nedić, Sreten and Simeunović, Predrag and Borozan, Sunčica and Nedić, Svetlana and Bojkovski, Jovan and Kirovski, Danijela and Vujanac, Ivan",
year = "2021",
abstract = "This study investigated the effects of dietary supplementation with chestnut tannins (CNT) on metabolic and antioxidant status of prepartum cows along with their colostrum quality. Pregnant multiparous Holstein cows were paired according to parity and body condition score, and assigned either to a diet supplemented with 20 g/d of commercially available product containing chestnut tannins (CNT, n=20) or to an unsupplemented control diet (CON, n=20) for the last 25±2 d of pregnancy. Serum metabolite, insulin and antioxidant capacity indices were measured in blood samples taken at d 25 and d 5 before expected parturition. Chemical composition and IgG concentration were determined in colostrum samples collected from the first milking postpartum. The addition of CNT led to lower BUN (P=0.02) and consequently higher serum glucose (P=0.02) and insulin (P<0.01) concentrations which were associated with lower circulating NEFA (P<0.01) and BHBA (P<0.01) in CNT group than those of CON. The serum paraoxonase 1 (PON 1) activity and total antioxidant capacity (T-AOC) were higher at-5 d in CNT than in CON (P<0.01, P=0.03; respectively). Close-up CNT improved lactose percentage and IgG concentration (P=0.03, P=0.04; respectively) and tended to improve percentage of protein and SNF (Solid Not Fat) in primary colostrum (P=0.06, respectively), without affecting colostrum fat and total solid (P=0.98, P=0.43; respectively). Supplementation of CNT in the diet during close-up period did not have adverse effects on metabolic profiles prepartum. Instead, this feeding regimen was more beneficial to antioxidant capacity and colostrum quality than feeding the control diet.",
publisher = "Sciendo",
journal = "Annals of Animal Science",
title = "Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices",
volume = "21",
number = "2",
pages = "609-621",
doi = "10.2478/aoas-2020-0077"
}

Prodanović, R., Nedić, S., Simeunović, P., Borozan, S., Nedić, S., Bojkovski, J., Kirovski, D.,& Vujanac, I.. (2021). Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices. in Annals of Animal Science
Sciendo., 21(2), 609-621.
https://doi.org/10.2478/aoas-2020-0077

Prodanović R, Nedić S, Simeunović P, Borozan S, Nedić S, Bojkovski J, Kirovski D, Vujanac I. Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices. in Annals of Animal Science. 2021;21(2):609-621.
doi:10.2478/aoas-2020-0077 .

Prodanović, Radiša, Nedić, Sreten, Simeunović, Predrag, Borozan, Sunčica, Nedić, Svetlana, Bojkovski, Jovan, Kirovski, Danijela, Vujanac, Ivan, "Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices" in Annals of Animal Science, 21, no. 2 (2021):609-621,
https://doi.org/10.2478/aoas-2020-0077 . .

TY  - JOUR
AU  - Vasić, A.
AU  - Oșlobanu, L.E.
AU  - Marinov, M.
AU  - Crivei, L.A.
AU  - Rățoi, I.A.
AU  - Aniță, A.
AU  - Aniță, D.
AU  - Doroșencu, A.
AU  - Alexe, V.
AU  - Raileanu, Cristian
AU  - Simeunović, Predrag
AU  - Raileanu, Cristian
AU  - Falcuța, E.
AU  - Prioteasa, F.L.
AU  - Bojkovski, Jovan
AU  - Pavlović, I.
AU  - Mathis, A.
AU  - Tews, B.A.
AU  - Savuta, Gheorghe
AU  - Veronesi, E.
AU  - Silaghi, Cornelia
AU  - Cimpan, A.
AU  - Ciucă, L.
AU  - Cojkić, A.
AU  - Drašković, Vladimir
AU  - Đurić, M.
AU  - Glavinić, Uroš
AU  - Ivanescu, M.L.
AU  - Kavran, M.
AU  - Lupu, A.
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Radanović, Oliver
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Zdravković, Nemanja
AU  - Vaselek, S.
AU  - SCOPES, AMSAR training group
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1720
AB  - West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.
PB  - MDPI AG
T2  - Tropical Medicine and Infectious Disease
T1  - Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016
VL  - 4
IS  - 3
SP  - 116
DO  - 10.3390/tropicalmed4030116
ER  - 

@article{
author = "Vasić, A. and Oșlobanu, L.E. and Marinov, M. and Crivei, L.A. and Rățoi, I.A. and Aniță, A. and Aniță, D. and Doroșencu, A. and Alexe, V. and Raileanu, Cristian and Simeunović, Predrag and Raileanu, Cristian and Falcuța, E. and Prioteasa, F.L. and Bojkovski, Jovan and Pavlović, I. and Mathis, A. and Tews, B.A. and Savuta, Gheorghe and Veronesi, E. and Silaghi, Cornelia and Cimpan, A. and Ciucă, L. and Cojkić, A. and Drašković, Vladimir and Đurić, M. and Glavinić, Uroš and Ivanescu, M.L. and Kavran, M. and Lupu, A. and Mindru, Raluca and Porea, Daniela and Radanović, Oliver and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Zdravković, Nemanja and Vaselek, S. and SCOPES, AMSAR training group",
year = "2019",
abstract = "West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.",
publisher = "MDPI AG",
journal = "Tropical Medicine and Infectious Disease",
title = "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016",
volume = "4",
number = "3",
pages = "116",
doi = "10.3390/tropicalmed4030116"
}

Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, C., Simeunović, P., Raileanu, C., Falcuța, E., Prioteasa, F.L., Bojkovski, J., Pavlović, I., Mathis, A., Tews, B.A., Savuta, G., Veronesi, E., Silaghi, C., Cimpan, A., Ciucă, L., Cojkić, A., Drašković, V., Đurić, M., Glavinić, U., Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, R., Porea, D., Radanović, O., Ristanić, M., Roman, C., Stanišić, L., Zdravković, N., Vaselek, S.,& SCOPES, A. t. g.. (2019). Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease
MDPI AG., 4(3), 116.
https://doi.org/10.3390/tropicalmed4030116

Vasić A, Oșlobanu L, Marinov M, Crivei L, Rățoi I, Aniță A, Aniță D, Doroșencu A, Alexe V, Raileanu C, Simeunović P, Raileanu C, Falcuța E, Prioteasa F, Bojkovski J, Pavlović I, Mathis A, Tews B, Savuta G, Veronesi E, Silaghi C, Cimpan A, Ciucă L, Cojkić A, Drašković V, Đurić M, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Radanović O, Ristanić M, Roman C, Stanišić L, Zdravković N, Vaselek S, SCOPES ATG. Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease. 2019;4(3):116.
doi:10.3390/tropicalmed4030116 .

Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, Cristian, Simeunović, Predrag, Raileanu, Cristian, Falcuța, E., Prioteasa, F.L., Bojkovski, Jovan, Pavlović, I., Mathis, A., Tews, B.A., Savuta, Gheorghe, Veronesi, E., Silaghi, Cornelia, Cimpan, A., Ciucă, L., Cojkić, A., Drašković, Vladimir, Đurić, M., Glavinić, Uroš, Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, Raluca, Porea, Daniela, Radanović, Oliver, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Zdravković, Nemanja, Vaselek, S., SCOPES, AMSAR training group, "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016" in Tropical Medicine and Infectious Disease, 4, no. 3 (2019):116,
https://doi.org/10.3390/tropicalmed4030116 . .

TY  - JOUR
AU  - Vasić, Ana
AU  - Zdravković, Nemanja
AU  - Anita, Dragos
AU  - Bojkovski, Jovan
AU  - Marinov, Mihai
AU  - Mathis, Alexander
AU  - Niculaua, Marius
AU  - Oslobanu, Elena Luanda
AU  - Pavlović, Ivan
AU  - Petrić, Dusan
AU  - Pflueger, Valentin
AU  - Pudar, Dubravka
AU  - Savuta, Gheorghe
AU  - Simeunović, Predrag
AU  - Veronesi, Eva
AU  - Silaghi, Cornelia
AU  - Anita, Adriana
AU  - Anton, Ioana Alexandra
AU  - Cimpan, Andrei
AU  - Ciuca, Lavinia
AU  - Crivei, Luciana
AU  - Cojkić, Aleksandar
AU  - Davitkov, Darko
AU  - Drašković, Vladimir
AU  - Gajić, Bojan
AU  - Glavinić, Uroš
AU  - Ivanescu, Maria-Larisa
AU  - Kavran, Mihaela
AU  - Lupu, Andrei-Cristian
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Prodanović, Radiša
AU  - Radanović, Oliver
AU  - Raileanu, Cristian
AU  - Raileanu, Stefan
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Vaselek, Slavica
AU  - Đurić, Miloje
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1740
AB  - BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia
VL  - 12
SP  - 61
DO  - 10.1186/s13071-019-3292-3
ER  - 

@article{
author = "Vasić, Ana and Zdravković, Nemanja and Anita, Dragos and Bojkovski, Jovan and Marinov, Mihai and Mathis, Alexander and Niculaua, Marius and Oslobanu, Elena Luanda and Pavlović, Ivan and Petrić, Dusan and Pflueger, Valentin and Pudar, Dubravka and Savuta, Gheorghe and Simeunović, Predrag and Veronesi, Eva and Silaghi, Cornelia and Anita, Adriana and Anton, Ioana Alexandra and Cimpan, Andrei and Ciuca, Lavinia and Crivei, Luciana and Cojkić, Aleksandar and Davitkov, Darko and Drašković, Vladimir and Gajić, Bojan and Glavinić, Uroš and Ivanescu, Maria-Larisa and Kavran, Mihaela and Lupu, Andrei-Cristian and Mindru, Raluca and Porea, Daniela and Prodanović, Radiša and Radanović, Oliver and Raileanu, Cristian and Raileanu, Stefan and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Vaselek, Slavica and Đurić, Miloje",
year = "2019",
abstract = "BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia",
volume = "12",
pages = "61",
doi = "10.1186/s13071-019-3292-3"
}

Vasić, A., Zdravković, N., Anita, D., Bojkovski, J., Marinov, M., Mathis, A., Niculaua, M., Oslobanu, E. L., Pavlović, I., Petrić, D., Pflueger, V., Pudar, D., Savuta, G., Simeunović, P., Veronesi, E., Silaghi, C., Anita, A., Anton, I. A., Cimpan, A., Ciuca, L., Crivei, L., Cojkić, A., Davitkov, D., Drašković, V., Gajić, B., Glavinić, U., Ivanescu, M., Kavran, M., Lupu, A., Mindru, R., Porea, D., Prodanović, R., Radanović, O., Raileanu, C., Raileanu, S., Ristanić, M., Roman, C., Stanišić, L., Vaselek, S.,& Đurić, M.. (2019). Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors
BMC, London., 12, 61.
https://doi.org/10.1186/s13071-019-3292-3

Vasić A, Zdravković N, Anita D, Bojkovski J, Marinov M, Mathis A, Niculaua M, Oslobanu EL, Pavlović I, Petrić D, Pflueger V, Pudar D, Savuta G, Simeunović P, Veronesi E, Silaghi C, Anita A, Anton IA, Cimpan A, Ciuca L, Crivei L, Cojkić A, Davitkov D, Drašković V, Gajić B, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Prodanović R, Radanović O, Raileanu C, Raileanu S, Ristanić M, Roman C, Stanišić L, Vaselek S, Đurić M. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors. 2019;12:61.
doi:10.1186/s13071-019-3292-3 .

Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia" in Parasites & Vectors, 12 (2019):61,
https://doi.org/10.1186/s13071-019-3292-3 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Simeunović, Predrag
AU  - Lakić, Nada
AU  - Radović, Ivica
AU  - Soković, Marina
AU  - Van Griensven, Leo J. L. D.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1630
AB  - This study was done to discover any beneficial effect of a medicinal mushroom Agaricus brasiliensis extract on the honey bee. Firstly, a laboratory experiment was conducted on 640 bees reared in 32 single-use plastic rearing cups. A. brasiliensis extract proved safe in all doses tested (50, 100 and 150 mg/kg/day) irrespective of feeding mode (sugar syrup or candy). Secondly, a three-year field experiment was conducted on 26 colonies treated with a single dose of A. brasiliensis extract (100 mg/kg/day) added to syrup. Each year the colonies were treated once in autumn and twice in spring. The treatments significantly increased colony strength parameters: brood rearing improvement and adult population growth were noticed more often than the increase in honey production and pollen reserves. These positive effects were mainly observed in April. In conclusion, A. brasiliensis extract is safe for the bees and helps maintaining strong colonies, especially in spring.
PB  - Acad Brasileira De Ciencias, Rio Janeiro
T2  - Anais da Academia Brasileira de Ciencias
T1  - The effect of Agaricus brasiliensis extract supplementation on honey bee colonies
VL  - 90
IS  - 1
SP  - 219
EP  - 229
DO  - 10.1590/0001-3765201820150182
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Simeunović, Predrag and Lakić, Nada and Radović, Ivica and Soković, Marina and Van Griensven, Leo J. L. D.",
year = "2018",
abstract = "This study was done to discover any beneficial effect of a medicinal mushroom Agaricus brasiliensis extract on the honey bee. Firstly, a laboratory experiment was conducted on 640 bees reared in 32 single-use plastic rearing cups. A. brasiliensis extract proved safe in all doses tested (50, 100 and 150 mg/kg/day) irrespective of feeding mode (sugar syrup or candy). Secondly, a three-year field experiment was conducted on 26 colonies treated with a single dose of A. brasiliensis extract (100 mg/kg/day) added to syrup. Each year the colonies were treated once in autumn and twice in spring. The treatments significantly increased colony strength parameters: brood rearing improvement and adult population growth were noticed more often than the increase in honey production and pollen reserves. These positive effects were mainly observed in April. In conclusion, A. brasiliensis extract is safe for the bees and helps maintaining strong colonies, especially in spring.",
publisher = "Acad Brasileira De Ciencias, Rio Janeiro",
journal = "Anais da Academia Brasileira de Ciencias",
title = "The effect of Agaricus brasiliensis extract supplementation on honey bee colonies",
volume = "90",
number = "1",
pages = "219-229",
doi = "10.1590/0001-3765201820150182"
}

Stevanović, J., Stanimirović, Z., Simeunović, P., Lakić, N., Radović, I., Soković, M.,& Van Griensven, L. J. L. D.. (2018). The effect of Agaricus brasiliensis extract supplementation on honey bee colonies. in Anais da Academia Brasileira de Ciencias
Acad Brasileira De Ciencias, Rio Janeiro., 90(1), 219-229.
https://doi.org/10.1590/0001-3765201820150182

Stevanović J, Stanimirović Z, Simeunović P, Lakić N, Radović I, Soković M, Van Griensven LJLD. The effect of Agaricus brasiliensis extract supplementation on honey bee colonies. in Anais da Academia Brasileira de Ciencias. 2018;90(1):219-229.
doi:10.1590/0001-3765201820150182 .

Stevanović, Jevrosima, Stanimirović, Zoran, Simeunović, Predrag, Lakić, Nada, Radović, Ivica, Soković, Marina, Van Griensven, Leo J. L. D., "The effect of Agaricus brasiliensis extract supplementation on honey bee colonies" in Anais da Academia Brasileira de Ciencias, 90, no. 1 (2018):219-229,
https://doi.org/10.1590/0001-3765201820150182 . .

TY  - JOUR
AU  - Stanišić, Ljubodrag
AU  - Dimitrijević, Vladimir
AU  - Simeunović, Predrag
AU  - Glavinić, Uroš
AU  - Jovanović, Biljana
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1474
AB  - The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed
VL  - 49
IS  - 1
SP  - 21
EP  - 30
DO  - 10.2298/GENSR1701021S
ER  - 

@article{
author = "Stanišić, Ljubodrag and Dimitrijević, Vladimir and Simeunović, Predrag and Glavinić, Uroš and Jovanović, Biljana and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2017",
abstract = "The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed",
volume = "49",
number = "1",
pages = "21-30",
doi = "10.2298/GENSR1701021S"
}

Stanišić, L., Dimitrijević, V., Simeunović, P., Glavinić, U., Jovanović, B., Stevanović, J.,& Stanimirović, Z.. (2017). Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 21-30.
https://doi.org/10.2298/GENSR1701021S

Stanišić L, Dimitrijević V, Simeunović P, Glavinić U, Jovanović B, Stevanović J, Stanimirović Z. Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed. in Genetika. 2017;49(1):21-30.
doi:10.2298/GENSR1701021S .

Stanišić, Ljubodrag, Dimitrijević, Vladimir, Simeunović, Predrag, Glavinić, Uroš, Jovanović, Biljana, Stevanović, Jevrosima, Stanimirović, Zoran, "Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed" in Genetika, 49, no. 1 (2017):21-30,
https://doi.org/10.2298/GENSR1701021S . .

TY  - JOUR
AU  - Stanišić, Ljubodrag
AU  - Aleksić, J. M.
AU  - Dimitrijević, Vladimir
AU  - Simeunović, Predrag
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1482
AB  - The Balkan donkey (Equus asinus L.) is commonly regarded as a large-sized, unselected, unstructured and traditionally managed donkey breed. We assessed the current genetic status of the three largest E. asinus populations in the central Balkans (Serbia) by analysing the variability of nuclear microsatellites and the mitochondrial (mtDNA) control region of 77 and 49 individuals respectively. We further analysed our mtDNA dataset along with 209 published mtDNA sequences of ancient and modern individuals from 19 European and African populations to provide new insights into the origin and the history of the Balkan donkey. Serbian donkey populations are highly genetically diverse at both the nuclear and mtDNA levels despite severe population decline. Traditional Balkan donkeys in Serbia are rather heterogeneous; we found two groups of individuals with similar phenotypic features, somewhat distinct nuclear backgrounds and different proportions of mtDNA haplotypes belonging to matrilineal Clades 1 and 2. Another group, characterized by larger body size, different coat colour, distinct nuclear gene pool and predominantly Clade 2 haplotypes, was delineated as the Banat donkey breed. The maternal landscape of the large Balkan donkey population is highly heterogeneous and more complex than previously thought. Given the two independent domestication events in donkeys, multiple waves of introductions into the Balkans from Greece are hypothesized. Clade 2 donkeys probably appeared in Greece prior to those belonging to Clade 1, whereas expansion and diversification of Clade 1 donkeys within the Balkans predated that of Clade 2 donkeys.
PB  - Wiley, Hoboken
T2  - Animal Genetics
T1  - New insights into the origin and the genetic status of the Balkan donkey from Serbia
VL  - 48
IS  - 5
SP  - 580
EP  - 590
DO  - 10.1111/age.12589
ER  - 

@article{
author = "Stanišić, Ljubodrag and Aleksić, J. M. and Dimitrijević, Vladimir and Simeunović, Predrag and Glavinić, Uroš and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2017",
abstract = "The Balkan donkey (Equus asinus L.) is commonly regarded as a large-sized, unselected, unstructured and traditionally managed donkey breed. We assessed the current genetic status of the three largest E. asinus populations in the central Balkans (Serbia) by analysing the variability of nuclear microsatellites and the mitochondrial (mtDNA) control region of 77 and 49 individuals respectively. We further analysed our mtDNA dataset along with 209 published mtDNA sequences of ancient and modern individuals from 19 European and African populations to provide new insights into the origin and the history of the Balkan donkey. Serbian donkey populations are highly genetically diverse at both the nuclear and mtDNA levels despite severe population decline. Traditional Balkan donkeys in Serbia are rather heterogeneous; we found two groups of individuals with similar phenotypic features, somewhat distinct nuclear backgrounds and different proportions of mtDNA haplotypes belonging to matrilineal Clades 1 and 2. Another group, characterized by larger body size, different coat colour, distinct nuclear gene pool and predominantly Clade 2 haplotypes, was delineated as the Banat donkey breed. The maternal landscape of the large Balkan donkey population is highly heterogeneous and more complex than previously thought. Given the two independent domestication events in donkeys, multiple waves of introductions into the Balkans from Greece are hypothesized. Clade 2 donkeys probably appeared in Greece prior to those belonging to Clade 1, whereas expansion and diversification of Clade 1 donkeys within the Balkans predated that of Clade 2 donkeys.",
publisher = "Wiley, Hoboken",
journal = "Animal Genetics",
title = "New insights into the origin and the genetic status of the Balkan donkey from Serbia",
volume = "48",
number = "5",
pages = "580-590",
doi = "10.1111/age.12589"
}

Stanišić, L., Aleksić, J. M., Dimitrijević, V., Simeunović, P., Glavinić, U., Stevanović, J.,& Stanimirović, Z.. (2017). New insights into the origin and the genetic status of the Balkan donkey from Serbia. in Animal Genetics
Wiley, Hoboken., 48(5), 580-590.
https://doi.org/10.1111/age.12589

Stanišić L, Aleksić JM, Dimitrijević V, Simeunović P, Glavinić U, Stevanović J, Stanimirović Z. New insights into the origin and the genetic status of the Balkan donkey from Serbia. in Animal Genetics. 2017;48(5):580-590.
doi:10.1111/age.12589 .

Stanišić, Ljubodrag, Aleksić, J. M., Dimitrijević, Vladimir, Simeunović, Predrag, Glavinić, Uroš, Stevanović, Jevrosima, Stanimirović, Zoran, "New insights into the origin and the genetic status of the Balkan donkey from Serbia" in Animal Genetics, 48, no. 5 (2017):580-590,
https://doi.org/10.1111/age.12589 . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Bojkovski, Jovan
AU  - Simeunović, Predrag
AU  - Palamarević, Milija
AU  - Nedić, Sreten
AU  - Celeska, Irena
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1536
AB  - This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Field trial on glucose-induced insulin response in high-yielding dairy cows under different environmental temperatures
VL  - 67
IS  - 3
SP  - 366
EP  - 382
DO  - 10.1515/acve-2017-0030
ER  - 

@article{
author = "Vujanac, Ivan and Prodanović, Radiša and Korićanac, Goran and Bojkovski, Jovan and Simeunović, Predrag and Palamarević, Milija and Nedić, Sreten and Celeska, Irena and Kirovski, Danijela",
year = "2017",
abstract = "This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Field trial on glucose-induced insulin response in high-yielding dairy cows under different environmental temperatures",
volume = "67",
number = "3",
pages = "366-382",
doi = "10.1515/acve-2017-0030"
}

Vujanac, I., Prodanović, R., Korićanac, G., Bojkovski, J., Simeunović, P., Palamarević, M., Nedić, S., Celeska, I.,& Kirovski, D.. (2017). Field trial on glucose-induced insulin response in high-yielding dairy cows under different environmental temperatures. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 366-382.
https://doi.org/10.1515/acve-2017-0030

Vujanac I, Prodanović R, Korićanac G, Bojkovski J, Simeunović P, Palamarević M, Nedić S, Celeska I, Kirovski D. Field trial on glucose-induced insulin response in high-yielding dairy cows under different environmental temperatures. in Acta Veterinaria-Beograd. 2017;67(3):366-382.
doi:10.1515/acve-2017-0030 .

Vujanac, Ivan, Prodanović, Radiša, Korićanac, Goran, Bojkovski, Jovan, Simeunović, Predrag, Palamarević, Milija, Nedić, Sreten, Celeska, Irena, Kirovski, Danijela, "Field trial on glucose-induced insulin response in high-yielding dairy cows under different environmental temperatures" in Acta Veterinaria-Beograd, 67, no. 3 (2017):366-382,
https://doi.org/10.1515/acve-2017-0030 . .

TY  - JOUR
AU  - Đurić, Spomenka
AU  - Simeunović, Predrag
AU  - Mirilović, Milorad
AU  - Stevanović, Jevrosima
AU  - Glavinić, Uroš
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1504
AB  - Bluetongue, a vector-born disease caused by the Bluetongue virus (BTV) and transmitted by Culicoides biting midges, is considered to be one of the most important diseases of domestic ruminants. The first outbreak of bluetongue in Serbia was reported in 2001, when BTV serotype 9 was identified in sampled materials. In 2014, outbreak of BTV-4 in Serbia caused considerable economic losses affecting sheep, cattle and goats. During this outbreak, BTV-4 was recorded in 644 outbreaks within 49 municipalities, part of 17 administrative regions. From the total number of sheep kept in areas affected by bluetongue (n=1 748 110), 2 083 cases (0.2%) were proven to be BTV-4 infected. Total of 206 infected cattle and 24 infected goats were reported during this investigation period, which represents 0.06% and 0.03% of the total number of cattle and goats kept in affected areas, respectively. The highest incidence of infected sheep, cattle and goats was recorded on the territory covered by veterinary institute of Nis. Recorded lethality in cattle, sheep and goats was 18.45% (n=38), 48.10% (n=1002) and 54.17% (n=13), respectively. The peak of the outbreak was in September and October when 94.43% of the confirmed positive cases, regardless of the species, was recorded. Monitoring of bluetongue disease in Serbia relies on active surveillance programmes aimed at: (i) identification and tracing of susceptible and potentially infected animals and (ii) detection, distribution and prevalence of insect vectors. Vaccination of sheep is planned to be implemented as a control measure against bluetongue in Serbia.
PB  - Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje
T2  - Macedonian Veterinary Review
T1  - Retrospective analysis of the bluetongue outbreak in Serbia
VL  - 40
IS  - 1
SP  - 21
EP  - 27
DO  - 10.1515/macvetrev-2016-0094
ER  - 

@article{
author = "Đurić, Spomenka and Simeunović, Predrag and Mirilović, Milorad and Stevanović, Jevrosima and Glavinić, Uroš and Vejnović, Branislav and Stanimirović, Zoran",
year = "2017",
abstract = "Bluetongue, a vector-born disease caused by the Bluetongue virus (BTV) and transmitted by Culicoides biting midges, is considered to be one of the most important diseases of domestic ruminants. The first outbreak of bluetongue in Serbia was reported in 2001, when BTV serotype 9 was identified in sampled materials. In 2014, outbreak of BTV-4 in Serbia caused considerable economic losses affecting sheep, cattle and goats. During this outbreak, BTV-4 was recorded in 644 outbreaks within 49 municipalities, part of 17 administrative regions. From the total number of sheep kept in areas affected by bluetongue (n=1 748 110), 2 083 cases (0.2%) were proven to be BTV-4 infected. Total of 206 infected cattle and 24 infected goats were reported during this investigation period, which represents 0.06% and 0.03% of the total number of cattle and goats kept in affected areas, respectively. The highest incidence of infected sheep, cattle and goats was recorded on the territory covered by veterinary institute of Nis. Recorded lethality in cattle, sheep and goats was 18.45% (n=38), 48.10% (n=1002) and 54.17% (n=13), respectively. The peak of the outbreak was in September and October when 94.43% of the confirmed positive cases, regardless of the species, was recorded. Monitoring of bluetongue disease in Serbia relies on active surveillance programmes aimed at: (i) identification and tracing of susceptible and potentially infected animals and (ii) detection, distribution and prevalence of insect vectors. Vaccination of sheep is planned to be implemented as a control measure against bluetongue in Serbia.",
publisher = "Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje",
journal = "Macedonian Veterinary Review",
title = "Retrospective analysis of the bluetongue outbreak in Serbia",
volume = "40",
number = "1",
pages = "21-27",
doi = "10.1515/macvetrev-2016-0094"
}

Đurić, S., Simeunović, P., Mirilović, M., Stevanović, J., Glavinić, U., Vejnović, B.,& Stanimirović, Z.. (2017). Retrospective analysis of the bluetongue outbreak in Serbia. in Macedonian Veterinary Review
Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje., 40(1), 21-27.
https://doi.org/10.1515/macvetrev-2016-0094

Đurić S, Simeunović P, Mirilović M, Stevanović J, Glavinić U, Vejnović B, Stanimirović Z. Retrospective analysis of the bluetongue outbreak in Serbia. in Macedonian Veterinary Review. 2017;40(1):21-27.
doi:10.1515/macvetrev-2016-0094 .

Đurić, Spomenka, Simeunović, Predrag, Mirilović, Milorad, Stevanović, Jevrosima, Glavinić, Uroš, Vejnović, Branislav, Stanimirović, Zoran, "Retrospective analysis of the bluetongue outbreak in Serbia" in Macedonian Veterinary Review, 40, no. 1 (2017):21-27,
https://doi.org/10.1515/macvetrev-2016-0094 . .

TY  - CONF
AU  - Cojkić, Aleksandar
AU  - Čobanović, Nikola
AU  - Simeunović, Predrag
AU  - Savić, Mila
AU  - Petrujkić, Branko
AU  - Vujanac, Ivan
AU  - Bojkovski, Jovan
AU  - Becskei, Zsolt
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2817
PB  - Požarevac : Sitograf RM
C3  - 13. simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 28 - 30. maj 2015.
T1  - Kompleks respiratornih oboljenja svinja (porcine respiratory diseases complex)- virusni uzročnici
SP  - 48
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2817
ER  - 

@conference{
author = "Cojkić, Aleksandar and Čobanović, Nikola and Simeunović, Predrag and Savić, Mila and Petrujkić, Branko and Vujanac, Ivan and Bojkovski, Jovan and Becskei, Zsolt",
year = "2015",
publisher = "Požarevac : Sitograf RM",
journal = "13. simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 28 - 30. maj 2015.",
title = "Kompleks respiratornih oboljenja svinja (porcine respiratory diseases complex)- virusni uzročnici",
pages = "48-56",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2817"
}

Cojkić, A., Čobanović, N., Simeunović, P., Savić, M., Petrujkić, B., Vujanac, I., Bojkovski, J.,& Becskei, Z.. (2015). Kompleks respiratornih oboljenja svinja (porcine respiratory diseases complex)- virusni uzročnici. in 13. simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 28 - 30. maj 2015.
Požarevac : Sitograf RM., 48-56.
https://hdl.handle.net/21.15107/rcub_veterinar_2817

Cojkić A, Čobanović N, Simeunović P, Savić M, Petrujkić B, Vujanac I, Bojkovski J, Becskei Z. Kompleks respiratornih oboljenja svinja (porcine respiratory diseases complex)- virusni uzročnici. in 13. simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 28 - 30. maj 2015.. 2015;:48-56.
https://hdl.handle.net/21.15107/rcub_veterinar_2817 .

Cojkić, Aleksandar, Čobanović, Nikola, Simeunović, Predrag, Savić, Mila, Petrujkić, Branko, Vujanac, Ivan, Bojkovski, Jovan, Becskei, Zsolt, "Kompleks respiratornih oboljenja svinja (porcine respiratory diseases complex)- virusni uzročnici" in 13. simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 28 - 30. maj 2015. (2015):48-56,
https://hdl.handle.net/21.15107/rcub_veterinar_2817 .

TY  - JOUR
AU  - Stanišić, Ljubodrag
AU  - Dimitrijević, Vladimir
AU  - Simeunović, Predrag
AU  - Lakić, Nada
AU  - Radović, Ivica
AU  - Ivanković, Ante
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1174
AB  - The aim of the study was to establish morphometric, biochemical and hematological values for the endangered Balkan donkey breed (Serbia) and to explore the possible age dependence of the parameters tested. Inter-breed similarity of morphometric parameters was assessed by comparing the data obtained for the Balkan donkey with morphometric measurements of several previously characterized domestic donkey breeds. The study population included 74 donkeys, divided in two age groups (group A <= 3 years; group B > 3 years). In total, 18 morphometric, 13 hematological and 14 biochemical parameters were assessed. Significant morphometric differences (p < 0.05) in body length, head length, chest circumference and body weight were found between the two age groups. Significant differences in morphological parameters were revealed among the Balkan donkey and other donkey breeds (Catalonian, Croatian and Albanian), but results of cluster analysis demonstrated the smallest distance between the Balkan donkey and Albanian donkeys. The results of morphometric analyses showed consistency of the obtained values within the breed, and diversity as compared to other donkey breeds, and, thus, could be taken as referent for the Balkan donkey. Hematological and biochemical profiles obtained for the Balkan donkey were consistent with previous reports and within the recommended reference ranges. White blood cell, mid cell and granulocyte counts, showed significantly higher (p < 0.05) values in donkeys under 3 years of age, while the only biochemical parameter affected by age was alkaline phosphatase. The information gained through characterization of the Balkan donkey breed provides a basis for conservation and development of the breed standard.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological, biochemical and hematological characterization of endangered balkan donkey breed
VL  - 65
IS  - 1
SP  - 125
EP  - 136
DO  - 10.1515/acve-2015-0010
ER  - 

@article{
author = "Stanišić, Ljubodrag and Dimitrijević, Vladimir and Simeunović, Predrag and Lakić, Nada and Radović, Ivica and Ivanković, Ante and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "The aim of the study was to establish morphometric, biochemical and hematological values for the endangered Balkan donkey breed (Serbia) and to explore the possible age dependence of the parameters tested. Inter-breed similarity of morphometric parameters was assessed by comparing the data obtained for the Balkan donkey with morphometric measurements of several previously characterized domestic donkey breeds. The study population included 74 donkeys, divided in two age groups (group A <= 3 years; group B > 3 years). In total, 18 morphometric, 13 hematological and 14 biochemical parameters were assessed. Significant morphometric differences (p < 0.05) in body length, head length, chest circumference and body weight were found between the two age groups. Significant differences in morphological parameters were revealed among the Balkan donkey and other donkey breeds (Catalonian, Croatian and Albanian), but results of cluster analysis demonstrated the smallest distance between the Balkan donkey and Albanian donkeys. The results of morphometric analyses showed consistency of the obtained values within the breed, and diversity as compared to other donkey breeds, and, thus, could be taken as referent for the Balkan donkey. Hematological and biochemical profiles obtained for the Balkan donkey were consistent with previous reports and within the recommended reference ranges. White blood cell, mid cell and granulocyte counts, showed significantly higher (p < 0.05) values in donkeys under 3 years of age, while the only biochemical parameter affected by age was alkaline phosphatase. The information gained through characterization of the Balkan donkey breed provides a basis for conservation and development of the breed standard.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological, biochemical and hematological characterization of endangered balkan donkey breed",
volume = "65",
number = "1",
pages = "125-136",
doi = "10.1515/acve-2015-0010"
}

Stanišić, L., Dimitrijević, V., Simeunović, P., Lakić, N., Radović, I., Ivanković, A., Stevanović, J.,& Stanimirović, Z.. (2015). Morphological, biochemical and hematological characterization of endangered balkan donkey breed. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 125-136.
https://doi.org/10.1515/acve-2015-0010

Stanišić L, Dimitrijević V, Simeunović P, Lakić N, Radović I, Ivanković A, Stevanović J, Stanimirović Z. Morphological, biochemical and hematological characterization of endangered balkan donkey breed. in Acta Veterinaria-Beograd. 2015;65(1):125-136.
doi:10.1515/acve-2015-0010 .

Stanišić, Ljubodrag, Dimitrijević, Vladimir, Simeunović, Predrag, Lakić, Nada, Radović, Ivica, Ivanković, Ante, Stevanović, Jevrosima, Stanimirović, Zoran, "Morphological, biochemical and hematological characterization of endangered balkan donkey breed" in Acta Veterinaria-Beograd, 65, no. 1 (2015):125-136,
https://doi.org/10.1515/acve-2015-0010 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Bogunović, Danica
AU  - Stevanović, Jevrosima
AU  - Kulišić, Zoran
AU  - Simeunović, Predrag
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1152
AB  - Thelazia callipaeda is a parasitic nematode causing ocular infections in different mammalian species and humans, clinically manifested as lacrimation, conjunctivitis, keratitis, corneal opacity or corneal ulcer. In this paper, we reported six cases of autochthonous canine and feline thelaziosis on different localities in Serbia. Total of 285 parasites (85 males and 200 females) were collected from the eyes of infected animals (n=6) suffering from uni- or bilateral conjunctivitis, with the number of parasites ranging from 7 to 150 per animal. All parasites were morphologically identified as T. callipaeda, while molecular analyses of cytochrome oxidase 1 (cox1) gene revealed the presence of h1 haplotype, as the unique previously reported in other studies in Europe. Since T. callipaeda is a newly detected parasite in Serbia with the infective potential for humans, there is a necessity for animal owners education and cooperation among professional services in order to control this zoonosis.
AB  - Thelazia callipaeda je parazitska nematoda koja izaziva infekciju oka različitih vrsta sisara i ljudi, a koja se klinički manifestuje pojačanim suzenjem, pojavom konjuktivitisa, keratitisa, zamućenja rožnjače ili pojave ulcera na rožnjači. U ovom radu opisani su nalazi autohtone telazioze pasa i mačaka na šest različitih lokaliteta u Srbiji. Iz očiju inficiranih životinja (n=6) sa simptomima uni- ili bilateralnog konjuktivitisa sakupljeno je 285 parazita (85 mužjaka i 200 ženki), pri čemu se broj parazita po životinji kretao od 7 do 150. Svi paraziti morfološki su identifikovani kao T. callipaeda, dok su molekularne analize citohrom oksidaza 1 (cox1) gena utvrdile postojanje h1 haplotipa, koji je do sada i jedini utvrđeni haplotip ovog parazita u Evropi. S obzirom da se radi o novootkrivenom parazitu na teritoriji Srbije koji može inficirati i ljude, neophodno je uložiti napore u edukaciju vlasnika životinja i saradnju stručnih službi u cilju kontrole ove zoonoze.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia
T1  - Telazioza pasa i mačaka izazvana sa Thelazia callipaeda u Srbiji
VL  - 64
IS  - 4
SP  - 447
EP  - 455
DO  - 10.2478/acve-2014-0042
ER  - 

@article{
author = "Gajić, Bojan and Bogunović, Danica and Stevanović, Jevrosima and Kulišić, Zoran and Simeunović, Predrag and Stanimirović, Zoran",
year = "2014",
abstract = "Thelazia callipaeda is a parasitic nematode causing ocular infections in different mammalian species and humans, clinically manifested as lacrimation, conjunctivitis, keratitis, corneal opacity or corneal ulcer. In this paper, we reported six cases of autochthonous canine and feline thelaziosis on different localities in Serbia. Total of 285 parasites (85 males and 200 females) were collected from the eyes of infected animals (n=6) suffering from uni- or bilateral conjunctivitis, with the number of parasites ranging from 7 to 150 per animal. All parasites were morphologically identified as T. callipaeda, while molecular analyses of cytochrome oxidase 1 (cox1) gene revealed the presence of h1 haplotype, as the unique previously reported in other studies in Europe. Since T. callipaeda is a newly detected parasite in Serbia with the infective potential for humans, there is a necessity for animal owners education and cooperation among professional services in order to control this zoonosis., Thelazia callipaeda je parazitska nematoda koja izaziva infekciju oka različitih vrsta sisara i ljudi, a koja se klinički manifestuje pojačanim suzenjem, pojavom konjuktivitisa, keratitisa, zamućenja rožnjače ili pojave ulcera na rožnjači. U ovom radu opisani su nalazi autohtone telazioze pasa i mačaka na šest različitih lokaliteta u Srbiji. Iz očiju inficiranih životinja (n=6) sa simptomima uni- ili bilateralnog konjuktivitisa sakupljeno je 285 parazita (85 mužjaka i 200 ženki), pri čemu se broj parazita po životinji kretao od 7 do 150. Svi paraziti morfološki su identifikovani kao T. callipaeda, dok su molekularne analize citohrom oksidaza 1 (cox1) gena utvrdile postojanje h1 haplotipa, koji je do sada i jedini utvrđeni haplotip ovog parazita u Evropi. S obzirom da se radi o novootkrivenom parazitu na teritoriji Srbije koji može inficirati i ljude, neophodno je uložiti napore u edukaciju vlasnika životinja i saradnju stručnih službi u cilju kontrole ove zoonoze.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia, Telazioza pasa i mačaka izazvana sa Thelazia callipaeda u Srbiji",
volume = "64",
number = "4",
pages = "447-455",
doi = "10.2478/acve-2014-0042"
}

Gajić, B., Bogunović, D., Stevanović, J., Kulišić, Z., Simeunović, P.,& Stanimirović, Z.. (2014). Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(4), 447-455.
https://doi.org/10.2478/acve-2014-0042

Gajić B, Bogunović D, Stevanović J, Kulišić Z, Simeunović P, Stanimirović Z. Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia. in Acta Veterinaria-Beograd. 2014;64(4):447-455.
doi:10.2478/acve-2014-0042 .

Gajić, Bojan, Bogunović, Danica, Stevanović, Jevrosima, Kulišić, Zoran, Simeunović, Predrag, Stanimirović, Zoran, "Canine and feline thelaziosis caused by Thelazia callipaeda in Serbia" in Acta Veterinaria-Beograd, 64, no. 4 (2014):447-455,
https://doi.org/10.2478/acve-2014-0042 . .

TY  - JOUR
AU  - Simeunović, Predrag
AU  - Stevanović, Jevrosima
AU  - Ćirković, Dragan
AU  - Radojičić, Sonja
AU  - Lakić, Nada
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1096
AB  - The strength of the honey bee colony and the quality concerning brood production, number of worker bees and honey yield depend substantially upon the characteristics of the queen. Reproductive and productive capacities of the colony, as two important physiological qualities can depend a great deal on the age of the queen. Over the years, the laying ratio of queens could decrease depending on the age; therefore colonies headed by one-year-old queens have proven to be more efficient considering colony population and honey production than ones with older queens. Nosema ceranae infection is a possible reason for the decrease in reproductive capacities and the reduction in the honey production of infected colonies. With the help of numerous pheromones, the queen can preserve colony homeostasis, however exhausted by N. ceranae infection she might compromise the renewal of worker population. The aim of this research is to investigate the influence of N. c-eranae on reproduction and productivity of honey bee colonies headed by queens of different age. Experiment was conducted on 27 honey bee colonies equalized with regard to brood, food source, number of worker bees and Nosema infection. In the three year investigation period (2009-2012), Nosema detection, identification and spore load per each colony, as well as parameters of colony reproduction (such as uncapped and capped brood area, queen egg-laying rate and colony strength) and productivity (the total extracted honey and the winter honey stores) were recorded. Concerning the influence of the age of the queens on followed parameters we compared one, two and three-years old queens and found significantly higher reproductive and productive values in favour of younger ones. Significant differences regarding the effects of N. ceranae infection on productive and reproductive characteristics could be explained with a higher capacity of one year old queens to compensate the effects of Nosema infection; a capacity which gradually declines in the second and the third year.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony
VL  - 53
IS  - 5
SP  - 545
EP  - 554
DO  - 10.3896/IBRA.1.53.5.09
ER  - 

@article{
author = "Simeunović, Predrag and Stevanović, Jevrosima and Ćirković, Dragan and Radojičić, Sonja and Lakić, Nada and Stanišić, Ljubodrag and Stanimirović, Zoran",
year = "2014",
abstract = "The strength of the honey bee colony and the quality concerning brood production, number of worker bees and honey yield depend substantially upon the characteristics of the queen. Reproductive and productive capacities of the colony, as two important physiological qualities can depend a great deal on the age of the queen. Over the years, the laying ratio of queens could decrease depending on the age; therefore colonies headed by one-year-old queens have proven to be more efficient considering colony population and honey production than ones with older queens. Nosema ceranae infection is a possible reason for the decrease in reproductive capacities and the reduction in the honey production of infected colonies. With the help of numerous pheromones, the queen can preserve colony homeostasis, however exhausted by N. ceranae infection she might compromise the renewal of worker population. The aim of this research is to investigate the influence of N. c-eranae on reproduction and productivity of honey bee colonies headed by queens of different age. Experiment was conducted on 27 honey bee colonies equalized with regard to brood, food source, number of worker bees and Nosema infection. In the three year investigation period (2009-2012), Nosema detection, identification and spore load per each colony, as well as parameters of colony reproduction (such as uncapped and capped brood area, queen egg-laying rate and colony strength) and productivity (the total extracted honey and the winter honey stores) were recorded. Concerning the influence of the age of the queens on followed parameters we compared one, two and three-years old queens and found significantly higher reproductive and productive values in favour of younger ones. Significant differences regarding the effects of N. ceranae infection on productive and reproductive characteristics could be explained with a higher capacity of one year old queens to compensate the effects of Nosema infection; a capacity which gradually declines in the second and the third year.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony",
volume = "53",
number = "5",
pages = "545-554",
doi = "10.3896/IBRA.1.53.5.09"
}

Simeunović, P., Stevanović, J., Ćirković, D., Radojičić, S., Lakić, N., Stanišić, L.,& Stanimirović, Z.. (2014). Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 53(5), 545-554.
https://doi.org/10.3896/IBRA.1.53.5.09

Simeunović P, Stevanović J, Ćirković D, Radojičić S, Lakić N, Stanišić L, Stanimirović Z. Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. in Journal of Apicultural Research. 2014;53(5):545-554.
doi:10.3896/IBRA.1.53.5.09 .

Simeunović, Predrag, Stevanović, Jevrosima, Ćirković, Dragan, Radojičić, Sonja, Lakić, Nada, Stanišić, Ljubodrag, Stanimirović, Zoran, "Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony" in Journal of Apicultural Research, 53, no. 5 (2014):545-554,
https://doi.org/10.3896/IBRA.1.53.5.09 . .

TY  - JOUR
AU  - Simeunović, Predrag
AU  - Stevanović, Jevrosima
AU  - Vidanović, Dejan
AU  - Nišavić, Jakov
AU  - Radović, Dejan
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1144
AB  - In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.
AB  - Ovo istraživanje je obuhvatilo utvrđivanje prisustva virusa deformacije krila (DWV) i virusa akutne paralize pčela (ABPV) u 55 pčelinjih društava poreklom iz različitih delova teritorije Srbije, upotrebom 'real-time RT-PCR' metode bazirane na 'TaqMan' probama. Rezultati pokazuju prisustvo DWV na svakom ispitivanom lokalitetu, dok je ABPV bio prisutan u 10 od 11 ispitivanih pčelinjaka. Imajući u vidu ulogu ektoparazita Varroa destructor u prenošenju i aktivaciji pčelinjih virusa, visoka učestalost uzoraka pozitivnih na DWV (76,4%) i ABPV (61,8%) u asimptomatskim pčelinjim društvima može biti posledica nedovoljno efikasnog i zakasnelog tretmana protiv ove grinje. 'Realtime RT-PCR' metoda, opisana u ovom radu se pokazala kao najpogodnija metoda za ovakvu vrstu istraživanja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR
T1  - Ispitivanje prisustva virusa deformacije krila i virusa akutne paralize pčela u pčelinjim zajednicama iz Srbije upotrebom 'real-time RT-PCR' metode
VL  - 64
IS  - 1
SP  - 81
EP  - 92
DO  - 10.2478/acve-2014-0009
ER  - 

@article{
author = "Simeunović, Predrag and Stevanović, Jevrosima and Vidanović, Dejan and Nišavić, Jakov and Radović, Dejan and Stanišić, Ljubodrag and Stanimirović, Zoran",
year = "2014",
abstract = "In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation., Ovo istraživanje je obuhvatilo utvrđivanje prisustva virusa deformacije krila (DWV) i virusa akutne paralize pčela (ABPV) u 55 pčelinjih društava poreklom iz različitih delova teritorije Srbije, upotrebom 'real-time RT-PCR' metode bazirane na 'TaqMan' probama. Rezultati pokazuju prisustvo DWV na svakom ispitivanom lokalitetu, dok je ABPV bio prisutan u 10 od 11 ispitivanih pčelinjaka. Imajući u vidu ulogu ektoparazita Varroa destructor u prenošenju i aktivaciji pčelinjih virusa, visoka učestalost uzoraka pozitivnih na DWV (76,4%) i ABPV (61,8%) u asimptomatskim pčelinjim društvima može biti posledica nedovoljno efikasnog i zakasnelog tretmana protiv ove grinje. 'Realtime RT-PCR' metoda, opisana u ovom radu se pokazala kao najpogodnija metoda za ovakvu vrstu istraživanja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR, Ispitivanje prisustva virusa deformacije krila i virusa akutne paralize pčela u pčelinjim zajednicama iz Srbije upotrebom 'real-time RT-PCR' metode",
volume = "64",
number = "1",
pages = "81-92",
doi = "10.2478/acve-2014-0009"
}

Simeunović, P., Stevanović, J., Vidanović, D., Nišavić, J., Radović, D., Stanišić, L.,& Stanimirović, Z.. (2014). A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1), 81-92.
https://doi.org/10.2478/acve-2014-0009

Simeunović P, Stevanović J, Vidanović D, Nišavić J, Radović D, Stanišić L, Stanimirović Z. A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR. in Acta Veterinaria-Beograd. 2014;64(1):81-92.
doi:10.2478/acve-2014-0009 .

Simeunović, Predrag, Stevanović, Jevrosima, Vidanović, Dejan, Nišavić, Jakov, Radović, Dejan, Stanišić, Ljubodrag, Stanimirović, Zoran, "A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR" in Acta Veterinaria-Beograd, 64, no. 1 (2014):81-92,
https://doi.org/10.2478/acve-2014-0009 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Gajić, Bojan
AU  - Simeunović, Predrag
AU  - Đurić, Spomenka
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1148
AB  - Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues).
AB  - Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor
T1  - DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor
VL  - 64
IS  - 3
SP  - 349
EP  - 357
DO  - 10.2478/acve-2014-0033
ER  - 

@article{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Gajić, Bojan and Simeunović, Predrag and Đurić, Spomenka and Vejnović, Branislav and Stanimirović, Zoran",
year = "2014",
abstract = "Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues)., Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor, DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor",
volume = "64",
number = "3",
pages = "349-357",
doi = "10.2478/acve-2014-0033"
}

Glavinić, U., Stevanović, J., Gajić, B., Simeunović, P., Đurić, S., Vejnović, B.,& Stanimirović, Z.. (2014). Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3), 349-357.
https://doi.org/10.2478/acve-2014-0033

Glavinić U, Stevanović J, Gajić B, Simeunović P, Đurić S, Vejnović B, Stanimirović Z. Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd. 2014;64(3):349-357.
doi:10.2478/acve-2014-0033 .

Glavinić, Uroš, Stevanović, Jevrosima, Gajić, Bojan, Simeunović, Predrag, Đurić, Spomenka, Vejnović, Branislav, Stanimirović, Zoran, "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor" in Acta Veterinaria-Beograd, 64, no. 3 (2014):349-357,
https://doi.org/10.2478/acve-2014-0033 . .

TY  - JOUR
AU  - Dimitrijević, Vladimir
AU  - Stevanović, Jevrosima
AU  - Savić, Mila
AU  - Petrujkić, Branko
AU  - Simeunović, Predrag
AU  - Milošević, Ivan
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1061
AB  - The aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs. In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Annals of Animal Science
T1  - Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina
VL  - 13
IS  - 4
SP  - 715
EP  - 722
DO  - 10.2478/aoas-2013-0047
ER  - 

@article{
author = "Dimitrijević, Vladimir and Stevanović, Jevrosima and Savić, Mila and Petrujkić, Branko and Simeunović, Predrag and Milošević, Ivan and Stanimirović, Zoran",
year = "2013",
abstract = "The aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs. In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Annals of Animal Science",
title = "Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina",
volume = "13",
number = "4",
pages = "715-722",
doi = "10.2478/aoas-2013-0047"
}

Dimitrijević, V., Stevanović, J., Savić, M., Petrujkić, B., Simeunović, P., Milošević, I.,& Stanimirović, Z.. (2013). Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina. in Annals of Animal Science
Walter De Gruyter Gmbh, Berlin., 13(4), 715-722.
https://doi.org/10.2478/aoas-2013-0047

Dimitrijević V, Stevanović J, Savić M, Petrujkić B, Simeunović P, Milošević I, Stanimirović Z. Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina. in Annals of Animal Science. 2013;13(4):715-722.
doi:10.2478/aoas-2013-0047 .

Dimitrijević, Vladimir, Stevanović, Jevrosima, Savić, Mila, Petrujkić, Branko, Simeunović, Predrag, Milošević, Ivan, Stanimirović, Zoran, "Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina" in Annals of Animal Science, 13, no. 4 (2013):715-722,
https://doi.org/10.2478/aoas-2013-0047 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stanković, Aleksandar
AU  - Stevanović, Jevrosima
AU  - Simeunović, Predrag
AU  - Aleksić, Nevenka
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1074
AB  - Two microsporidia species of the Nosema genus cause nosemosis in the adult honeybee: N. apis and N. ceranae. For diagnostic purposes and the determination of infection level various microscopic and molecular biological methods are used. The aim of this research was to compare the reliability of the traditional microscopic assessment and two PCR techniques: simplex- and duplex-PCR. Honey bee samples were taken from 150 colonies. Microscopic examination, performed according to the recommendations of the OIE, revealed Nosema spores in 68.67% samples analysed, whilst with the simplex-PCR method all samples (100.0%) proved positive. On the other hand, duplex-PCR method used for the identification of Nosema species resulted in 84.0% positive samples, all of which were N. ceranae. Our recommendation of the simplex-PCR method for the monitoring of honey bees in field conditions is based on its higher reliability than the microscopic assessment in the detection of low-level infections, as well as its potential for the detection of vegetative Nosema sp. stages; thus the early detection and timely prevention of Nosema infection would be possible. Nosema species identification is simplest and most cost-effective if performed with the duplex-PCR analysis. However, the simplex-PCR is more reliable, thus, it is suggested that samples that were negative when assessed with microscopy and duplex-PCR analysis undergo simplex-PCR.
AB  - Dve vrste mikrosporidija roda Nosema uzročnici su nozemoze kod odrasle medonosne pčele: N. apis i N. ceranae. Za postavljanje dijagnoze i utvrđivanje stepena infekcije koristi se nekoliko mikroskopskih i molekularno-bioloških metoda. Cilj našeg rada bilo je poređenje pouzdanosti tradicionalne mikroskopske metode i dve PCR metode: simplex- i duplex-PCR. Pregledano je ukupno 150 uzoraka pčela. Mikroskopskim pregledom, obavljenim prema preporukama OIE, prisustvo spora Nosema utvrđeno je u 68,7% uzoraka. Međutim, simplex-PCR metodom dobijeni su pozitivni rezultati u svih 150 uzorka (100,0%). Sa druge strane, primenom duplex-PCR metode infekcija je ustanovljena kod 84,0 %; u svim slučajevima determinisana je vrsta N. ceranae. Veća pouzdanost simplex-PCR metode u odnosu na mikroskopski pregled, kako u otkrivanju infekcije malog intenziteta, kao i mogućnost detekcije vegetativnih oblika nozeme, navodi nas da preporučimo uvođenje simplex-PCR metode kao obavezne za praćenje stanja pčelinjih društava na terenu; time bi se postigla rano utvrđivanje prisustva infekcije i blagovremena prevencija njenog širenja. Specijska identifikacija mikrosporidija roda Nosema najjednostavnija je i najisplativija metodom duplex-PCR. Međutim, simplex-PCR ima veću pouzdanost, te preporučujemo da se uzorci koji su negativni na osnovu mikroskopskog pregleda i duplex-PCR analize ispitaju i simplex-PCR metodom.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Comparison of methods for detection of microsporidia species of the genus Nosema in honey bees (Apis mellifera)
T1  - Komparacija metoda za detekciju mikrosporidia iz roda Nosema kod medonosne pčele (Apis mellifera)
VL  - 6
IS  - 1
SP  - 19
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1074
ER  - 

@article{
author = "Glavinić, Uroš and Stanković, Aleksandar and Stevanović, Jevrosima and Simeunović, Predrag and Aleksić, Nevenka and Stanimirović, Zoran",
year = "2013",
abstract = "Two microsporidia species of the Nosema genus cause nosemosis in the adult honeybee: N. apis and N. ceranae. For diagnostic purposes and the determination of infection level various microscopic and molecular biological methods are used. The aim of this research was to compare the reliability of the traditional microscopic assessment and two PCR techniques: simplex- and duplex-PCR. Honey bee samples were taken from 150 colonies. Microscopic examination, performed according to the recommendations of the OIE, revealed Nosema spores in 68.67% samples analysed, whilst with the simplex-PCR method all samples (100.0%) proved positive. On the other hand, duplex-PCR method used for the identification of Nosema species resulted in 84.0% positive samples, all of which were N. ceranae. Our recommendation of the simplex-PCR method for the monitoring of honey bees in field conditions is based on its higher reliability than the microscopic assessment in the detection of low-level infections, as well as its potential for the detection of vegetative Nosema sp. stages; thus the early detection and timely prevention of Nosema infection would be possible. Nosema species identification is simplest and most cost-effective if performed with the duplex-PCR analysis. However, the simplex-PCR is more reliable, thus, it is suggested that samples that were negative when assessed with microscopy and duplex-PCR analysis undergo simplex-PCR., Dve vrste mikrosporidija roda Nosema uzročnici su nozemoze kod odrasle medonosne pčele: N. apis i N. ceranae. Za postavljanje dijagnoze i utvrđivanje stepena infekcije koristi se nekoliko mikroskopskih i molekularno-bioloških metoda. Cilj našeg rada bilo je poređenje pouzdanosti tradicionalne mikroskopske metode i dve PCR metode: simplex- i duplex-PCR. Pregledano je ukupno 150 uzoraka pčela. Mikroskopskim pregledom, obavljenim prema preporukama OIE, prisustvo spora Nosema utvrđeno je u 68,7% uzoraka. Međutim, simplex-PCR metodom dobijeni su pozitivni rezultati u svih 150 uzorka (100,0%). Sa druge strane, primenom duplex-PCR metode infekcija je ustanovljena kod 84,0 %; u svim slučajevima determinisana je vrsta N. ceranae. Veća pouzdanost simplex-PCR metode u odnosu na mikroskopski pregled, kako u otkrivanju infekcije malog intenziteta, kao i mogućnost detekcije vegetativnih oblika nozeme, navodi nas da preporučimo uvođenje simplex-PCR metode kao obavezne za praćenje stanja pčelinjih društava na terenu; time bi se postigla rano utvrđivanje prisustva infekcije i blagovremena prevencija njenog širenja. Specijska identifikacija mikrosporidija roda Nosema najjednostavnija je i najisplativija metodom duplex-PCR. Međutim, simplex-PCR ima veću pouzdanost, te preporučujemo da se uzorci koji su negativni na osnovu mikroskopskog pregleda i duplex-PCR analize ispitaju i simplex-PCR metodom.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Comparison of methods for detection of microsporidia species of the genus Nosema in honey bees (Apis mellifera), Komparacija metoda za detekciju mikrosporidia iz roda Nosema kod medonosne pčele (Apis mellifera)",
volume = "6",
number = "1",
pages = "19-27",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1074"
}

Glavinić, U., Stanković, A., Stevanović, J., Simeunović, P., Aleksić, N.,& Stanimirović, Z.. (2013). Comparison of methods for detection of microsporidia species of the genus Nosema in honey bees (Apis mellifera). in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 6(1), 19-27.
https://hdl.handle.net/21.15107/rcub_veterinar_1074

Glavinić U, Stanković A, Stevanović J, Simeunović P, Aleksić N, Stanimirović Z. Comparison of methods for detection of microsporidia species of the genus Nosema in honey bees (Apis mellifera). in Arhiv veterinarske medicine. 2013;6(1):19-27.
https://hdl.handle.net/21.15107/rcub_veterinar_1074 .

Glavinić, Uroš, Stanković, Aleksandar, Stevanović, Jevrosima, Simeunović, Predrag, Aleksić, Nevenka, Stanimirović, Zoran, "Comparison of methods for detection of microsporidia species of the genus Nosema in honey bees (Apis mellifera)" in Arhiv veterinarske medicine, 6, no. 1 (2013):19-27,
https://hdl.handle.net/21.15107/rcub_veterinar_1074 .

TY  - JOUR
AU  - Bošnjak, Jasna
AU  - Stevanov-Pavlović, Marija
AU  - Vučićević, Miloš
AU  - Stevanović, Jevrosima
AU  - Simeunović, Predrag
AU  - Resanović, Radmila
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1066
AB  - The aim of this study was to test the feasibility and efficacy of a non-invasive molecular method for gender identification of parrots, using different types of samples and the Chromo Helicase DNA-binding (CHD) gene as a molecular marker. DNA was isolated primarily from feathers and the amplification of the CHD gene was performed using 2550F/2718R primers. In order to compare the reliability of different sources of DNA, we used buccal swab, blood and feces. All sample types exerted successful sexing results with the exception of feces samples where the success rate was 25%. Sexing was successfully determined in 239 birds belonging to 32 species of parrots. In 6 species (Amazona finschi, A. leucocephala, Aratinga aurea, Bamardius zonarius, Coracopsis nigra and Nymphicus hollandicus), 2550F/2718R primers proved to work well for the first time. Species used in this study are on the IUCN red list of threatened species. Furthermore, Amazona finschi, A. leucocephala, Cacatua moluccensis and C. sulphurea are on the list of CITES Apendix I. Since the failure in reproduction is one of the main causes of illegal trafficking of parrots, the non-invasive and universal molecular sexing method we tested may be a very useful tool in the preservation of endangered parrot species.
PB  - Zoological Soc Pakistan, Lahore
T2  - Pakistan Journal of Zoology
T1  - Feasibility of non-invasive molecular method for sexing of parrots
VL  - 45
IS  - 3
SP  - 715
EP  - 720
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1066
ER  - 

@article{
author = "Bošnjak, Jasna and Stevanov-Pavlović, Marija and Vučićević, Miloš and Stevanović, Jevrosima and Simeunović, Predrag and Resanović, Radmila and Stanimirović, Zoran",
year = "2013",
abstract = "The aim of this study was to test the feasibility and efficacy of a non-invasive molecular method for gender identification of parrots, using different types of samples and the Chromo Helicase DNA-binding (CHD) gene as a molecular marker. DNA was isolated primarily from feathers and the amplification of the CHD gene was performed using 2550F/2718R primers. In order to compare the reliability of different sources of DNA, we used buccal swab, blood and feces. All sample types exerted successful sexing results with the exception of feces samples where the success rate was 25%. Sexing was successfully determined in 239 birds belonging to 32 species of parrots. In 6 species (Amazona finschi, A. leucocephala, Aratinga aurea, Bamardius zonarius, Coracopsis nigra and Nymphicus hollandicus), 2550F/2718R primers proved to work well for the first time. Species used in this study are on the IUCN red list of threatened species. Furthermore, Amazona finschi, A. leucocephala, Cacatua moluccensis and C. sulphurea are on the list of CITES Apendix I. Since the failure in reproduction is one of the main causes of illegal trafficking of parrots, the non-invasive and universal molecular sexing method we tested may be a very useful tool in the preservation of endangered parrot species.",
publisher = "Zoological Soc Pakistan, Lahore",
journal = "Pakistan Journal of Zoology",
title = "Feasibility of non-invasive molecular method for sexing of parrots",
volume = "45",
number = "3",
pages = "715-720",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1066"
}

Bošnjak, J., Stevanov-Pavlović, M., Vučićević, M., Stevanović, J., Simeunović, P., Resanović, R.,& Stanimirović, Z.. (2013). Feasibility of non-invasive molecular method for sexing of parrots. in Pakistan Journal of Zoology
Zoological Soc Pakistan, Lahore., 45(3), 715-720.
https://hdl.handle.net/21.15107/rcub_veterinar_1066

Bošnjak J, Stevanov-Pavlović M, Vučićević M, Stevanović J, Simeunović P, Resanović R, Stanimirović Z. Feasibility of non-invasive molecular method for sexing of parrots. in Pakistan Journal of Zoology. 2013;45(3):715-720.
https://hdl.handle.net/21.15107/rcub_veterinar_1066 .

Bošnjak, Jasna, Stevanov-Pavlović, Marija, Vučićević, Miloš, Stevanović, Jevrosima, Simeunović, Predrag, Resanović, Radmila, Stanimirović, Zoran, "Feasibility of non-invasive molecular method for sexing of parrots" in Pakistan Journal of Zoology, 45, no. 3 (2013):715-720,
https://hdl.handle.net/21.15107/rcub_veterinar_1066 .

TY  - JOUR
AU  - Dimitrijević, Vladimir
AU  - Trailović, Ružica
AU  - Petrujkić, Branko
AU  - Savić, Mila
AU  - Simeunović, Predrag
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/967
AB  - Application of the molecular genetic methods in forensic cases dealing with wild animals has significantly increased recently. These techniques are practically used in order to help solving four key problems : determination of kind of the wild animal, geographic origin, kinship ties and individual identification. In this work the first case of introducing the examination of polimorphism of microsatelite genetic markers within forensic analysis in the cases of poaching in Serbia is presented. The objectives of this forensic analysis was to determine if the meat confiscated during house search of the suspect comes from roebuck origin (Capreolus capreolus), which remains had been found by a game warden in the field during closed season, where the suspect denied the offense, claiming that the meat comes from other roebuck that had been shot during the previous hunting season. DNK was isolated from the skin and fur samples taken from the roebuck corpse found in the woods, as well as from the frozen meat found in the suspect's house. Both amplification and polimorphism examination of the eight microsatelite markers (ROE01, NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 AND RT27) were carried out. In all the examined samples, the same pattern of variability of the tested microsatelites was determined, that is it was proved that DNK profiles of the samples taken from roebuck corpse were identical to DNK profile of the meat sample found in the suspect's house. This result clearly indicates that all the examined biological samples originate from the same animal, and consequently represents forensically valid evidence in the case of roebuck poaching.
AB  - Primena molekularno-genetičkih tehnika u forenzičkim slučajevima koji uključuju divlje životinje u značajnom je porastu tokom poslednjih godina. Ove tehnike se u praktičnom radu na slučajevima primarno koriste u rešavanju četiri ključna problema: utvrđivanje vrste divlje životinje, određivanje geografskog porekla, utvrđivanje srodničkih veza i individualna identifikacija. U ovom radu predstavljamo prvi slučaj primene ispitivanja polimorfizma mikrosatelitskih genetičkih markera u okviru forenzičke analize slučaja krivolova u Srbiji. Cilj forenzičke analize bio je da se utvrdi da li meso zaplenjeno tokom pretresa kuće osumnjičenog potiče od srndaća (Capreolus capreolus), čiji su ostaci pronađeni od strane lovočuvara na terenu tokom perioda lovostaja, pri čemu je osumnjičeni negirao delo tvrdeći da meso potiče od drugog srndaća ubijenog tokom prethodne sezone lova. DNK je izolovana iz uzoraka kože i krzna uzetih sa leša srndaća nađenog u šumi i uzoraka zamrznutog mesa nađenog u kući osumnjičenog. Izvršena je amplifikacija i ispitivanje polimorfizma osam mikrosatelitskih markera (ROE01, NVHRT16, NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 i RT27). U svim ispitivanim uzorcima ustanovljen je isti obrazac varijabilnosti testiranih mikrosatelita, odnosno pokazano je da su DNK profili uzoraka uzetih sa leša srndaća identični DNK profilu uzorka mesa nađenog u kući osumnjičenog. Ovakav rezultat jasno ukazuje na to da su svi ispitivani biološki uzorci poticali od iste životinje i predstavlja forenzički validan dokaz u slučaju krivolova srndaća.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia
T1  - Molekularno-genetički pristup individualnoj identifikaciji srndaća u slučaju krivolova u Srbiji
VL  - 67
IS  - 3-4
SP  - 279
EP  - 287
DO  - 10.2298/VETGL1304279D
ER  - 

@article{
author = "Dimitrijević, Vladimir and Trailović, Ružica and Petrujkić, Branko and Savić, Mila and Simeunović, Predrag and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2013",
abstract = "Application of the molecular genetic methods in forensic cases dealing with wild animals has significantly increased recently. These techniques are practically used in order to help solving four key problems : determination of kind of the wild animal, geographic origin, kinship ties and individual identification. In this work the first case of introducing the examination of polimorphism of microsatelite genetic markers within forensic analysis in the cases of poaching in Serbia is presented. The objectives of this forensic analysis was to determine if the meat confiscated during house search of the suspect comes from roebuck origin (Capreolus capreolus), which remains had been found by a game warden in the field during closed season, where the suspect denied the offense, claiming that the meat comes from other roebuck that had been shot during the previous hunting season. DNK was isolated from the skin and fur samples taken from the roebuck corpse found in the woods, as well as from the frozen meat found in the suspect's house. Both amplification and polimorphism examination of the eight microsatelite markers (ROE01, NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 AND RT27) were carried out. In all the examined samples, the same pattern of variability of the tested microsatelites was determined, that is it was proved that DNK profiles of the samples taken from roebuck corpse were identical to DNK profile of the meat sample found in the suspect's house. This result clearly indicates that all the examined biological samples originate from the same animal, and consequently represents forensically valid evidence in the case of roebuck poaching., Primena molekularno-genetičkih tehnika u forenzičkim slučajevima koji uključuju divlje životinje u značajnom je porastu tokom poslednjih godina. Ove tehnike se u praktičnom radu na slučajevima primarno koriste u rešavanju četiri ključna problema: utvrđivanje vrste divlje životinje, određivanje geografskog porekla, utvrđivanje srodničkih veza i individualna identifikacija. U ovom radu predstavljamo prvi slučaj primene ispitivanja polimorfizma mikrosatelitskih genetičkih markera u okviru forenzičke analize slučaja krivolova u Srbiji. Cilj forenzičke analize bio je da se utvrdi da li meso zaplenjeno tokom pretresa kuće osumnjičenog potiče od srndaća (Capreolus capreolus), čiji su ostaci pronađeni od strane lovočuvara na terenu tokom perioda lovostaja, pri čemu je osumnjičeni negirao delo tvrdeći da meso potiče od drugog srndaća ubijenog tokom prethodne sezone lova. DNK je izolovana iz uzoraka kože i krzna uzetih sa leša srndaća nađenog u šumi i uzoraka zamrznutog mesa nađenog u kući osumnjičenog. Izvršena je amplifikacija i ispitivanje polimorfizma osam mikrosatelitskih markera (ROE01, NVHRT16, NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 i RT27). U svim ispitivanim uzorcima ustanovljen je isti obrazac varijabilnosti testiranih mikrosatelita, odnosno pokazano je da su DNK profili uzoraka uzetih sa leša srndaća identični DNK profilu uzorka mesa nađenog u kući osumnjičenog. Ovakav rezultat jasno ukazuje na to da su svi ispitivani biološki uzorci poticali od iste životinje i predstavlja forenzički validan dokaz u slučaju krivolova srndaća.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia, Molekularno-genetički pristup individualnoj identifikaciji srndaća u slučaju krivolova u Srbiji",
volume = "67",
number = "3-4",
pages = "279-287",
doi = "10.2298/VETGL1304279D"
}

Dimitrijević, V., Trailović, R., Petrujkić, B., Savić, M., Simeunović, P., Stevanović, J.,& Stanimirović, Z.. (2013). A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3-4), 279-287.
https://doi.org/10.2298/VETGL1304279D

Dimitrijević V, Trailović R, Petrujkić B, Savić M, Simeunović P, Stevanović J, Stanimirović Z. A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia. in Veterinarski Glasnik. 2013;67(3-4):279-287.
doi:10.2298/VETGL1304279D .

Dimitrijević, Vladimir, Trailović, Ružica, Petrujkić, Branko, Savić, Mila, Simeunović, Predrag, Stevanović, Jevrosima, Stanimirović, Zoran, "A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia" in Veterinarski Glasnik, 67, no. 3-4 (2013):279-287,
https://doi.org/10.2298/VETGL1304279D . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Radulović, Željko
AU  - Stevanović, Jevrosima
AU  - Kulišić, Zoran
AU  - Vučićević, Miloš
AU  - Simeunović, Predrag
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1050
AB  - Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis
VL  - 61
IS  - 1
SP  - 97
EP  - 105
DO  - 10.1007/s10493-013-9683-9
ER  - 

@article{
author = "Gajić, Bojan and Radulović, Željko and Stevanović, Jevrosima and Kulišić, Zoran and Vučićević, Miloš and Simeunović, Predrag and Stanimirović, Zoran",
year = "2013",
abstract = "Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis",
volume = "61",
number = "1",
pages = "97-105",
doi = "10.1007/s10493-013-9683-9"
}

Gajić, B., Radulović, Ž., Stevanović, J., Kulišić, Z., Vučićević, M., Simeunović, P.,& Stanimirović, Z.. (2013). Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology
Springer, Dordrecht., 61(1), 97-105.
https://doi.org/10.1007/s10493-013-9683-9

Gajić B, Radulović Ž, Stevanović J, Kulišić Z, Vučićević M, Simeunović P, Stanimirović Z. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology. 2013;61(1):97-105.
doi:10.1007/s10493-013-9683-9 .

Gajić, Bojan, Radulović, Željko, Stevanović, Jevrosima, Kulišić, Zoran, Vučićević, Miloš, Simeunović, Predrag, Stanimirović, Zoran, "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis" in Experimental and Applied Acarology, 61, no. 1 (2013):97-105,
https://doi.org/10.1007/s10493-013-9683-9 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Simeunović, Predrag
AU  - Gajić, Bojan
AU  - Lakić, Nada
AU  - Radović, Dejan
AU  - Fries, Ingemar
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1076
AB  - The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.
PB  - Springer France, Paris
T2  - Apidologie
T1  - Characteristics of Nosema ceranae infection in Serbian honey bee colonies
VL  - 44
IS  - 5
SP  - 522
EP  - 536
DO  - 10.1007/s13592-013-0203-z
ER  - 

@article{
author = "Stevanović, Jevrosima and Simeunović, Predrag and Gajić, Bojan and Lakić, Nada and Radović, Dejan and Fries, Ingemar and Stanimirović, Zoran",
year = "2013",
abstract = "The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008-2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008-2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranae-infected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.",
publisher = "Springer France, Paris",
journal = "Apidologie",
title = "Characteristics of Nosema ceranae infection in Serbian honey bee colonies",
volume = "44",
number = "5",
pages = "522-536",
doi = "10.1007/s13592-013-0203-z"
}

Stevanović, J., Simeunović, P., Gajić, B., Lakić, N., Radović, D., Fries, I.,& Stanimirović, Z.. (2013). Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie
Springer France, Paris., 44(5), 522-536.
https://doi.org/10.1007/s13592-013-0203-z

Stevanović J, Simeunović P, Gajić B, Lakić N, Radović D, Fries I, Stanimirović Z. Characteristics of Nosema ceranae infection in Serbian honey bee colonies. in Apidologie. 2013;44(5):522-536.
doi:10.1007/s13592-013-0203-z .

Stevanović, Jevrosima, Simeunović, Predrag, Gajić, Bojan, Lakić, Nada, Radović, Dejan, Fries, Ingemar, Stanimirović, Zoran, "Characteristics of Nosema ceranae infection in Serbian honey bee colonies" in Apidologie, 44, no. 5 (2013):522-536,
https://doi.org/10.1007/s13592-013-0203-z . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Simeunović, Predrag
AU  - Kulišić, Zoran
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/760
AB  - The hygienic behavior in honey bees is a dominant natural defense mechanism against brood diseases. In this study, the influence of sugar dusting treatments on hygienic behavior was evaluated in 44 strong honey bee colonies. Three doses of pulverized sugar, 20, 30 and 40 g, each applied at three-, seven- and fourteen-day intervals were tested. The percentage of cleaned cells (PCC) in the total number of those with pin-killed brood served as a measure of the hygienic potential. The effect was dependent on the frequency of treatments: all doses applied every third and seventh day significantly (p lt 0.001) decreased the PCC in comparison with the untreated control colonies. Nevertheless, sugar did not threaten the hygienic potential, as PPC values remained above 94% following all treatments. Thus, it can be concluded that the tested sugar treatments are safe and can be justifiably implemented into integrated pest management strategies to control Varroa destructor.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies
VL  - 63
IS  - 4
SP  - 1199
EP  - 1207
DO  - 10.2298/ABS1104199S
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Lakić, Nada and Aleksić, Nevenka and Simeunović, Predrag and Kulišić, Zoran",
year = "2011",
abstract = "The hygienic behavior in honey bees is a dominant natural defense mechanism against brood diseases. In this study, the influence of sugar dusting treatments on hygienic behavior was evaluated in 44 strong honey bee colonies. Three doses of pulverized sugar, 20, 30 and 40 g, each applied at three-, seven- and fourteen-day intervals were tested. The percentage of cleaned cells (PCC) in the total number of those with pin-killed brood served as a measure of the hygienic potential. The effect was dependent on the frequency of treatments: all doses applied every third and seventh day significantly (p lt 0.001) decreased the PCC in comparison with the untreated control colonies. Nevertheless, sugar did not threaten the hygienic potential, as PPC values remained above 94% following all treatments. Thus, it can be concluded that the tested sugar treatments are safe and can be justifiably implemented into integrated pest management strategies to control Varroa destructor.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies",
volume = "63",
number = "4",
pages = "1199-1207",
doi = "10.2298/ABS1104199S"
}

Stevanović, J., Stanimirović, Z., Lakić, N., Aleksić, N., Simeunović, P.,& Kulišić, Z.. (2011). Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(4), 1199-1207.
https://doi.org/10.2298/ABS1104199S

Stevanović J, Stanimirović Z, Lakić N, Aleksić N, Simeunović P, Kulišić Z. Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies. in Archives of Biological Sciences. 2011;63(4):1199-1207.
doi:10.2298/ABS1104199S .

Stevanović, Jevrosima, Stanimirović, Zoran, Lakić, Nada, Aleksić, Nevenka, Simeunović, Predrag, Kulišić, Zoran, "Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies" in Archives of Biological Sciences, 63, no. 4 (2011):1199-1207,
https://doi.org/10.2298/ABS1104199S . .