TY  - CONF
AU  - Šefer, Dragan
AU  - Perić, Dejan
AU  - Jakić-Dimić, Dobrila
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3013
AB  - Jaja predstavljaju važan izvor proteina, masti i mikroelemenata koji imaju značajnu ulogu u ishrani
ljudi. Proizvodnja i potrosnja jaja u svetu poslednjih decenija je u porastu. Konzumacija jaja dugo vremena
bila je vezana za negativne efekte na zdravlje ljudi, uglavnom zbog sadržaja holesterola. Međutim, danas je
poznato da na nivo holesterola u serumu utiče vise drugih faktora kao sto su genetska predispozicija,
hormonski status i hranidbene navike, a ne isključivo holesterol iz jaja. Poslednjih godina, namirnice koje se
svakodnevno koriste u ishrani ljudi nisu namenjene samo zadovoljenju potreba u osnovnim hranljivim
materijama nego se od hrane očekuje prevencija bolesti povezanih sa hranom i sticanje boljeg imunoloskog
statusa. Upotrebom specifičnih nutritivnih strategija moguće je proizvesti funkcionalnu hranu koja pored
osnovnih hranljivih materija sadrži i komponente koje učestvuju u očuvanju zdravlja i smanjenju rizika u
nastanku bolesti. Niska koncentracija selena u zemljistu, a posledično i u hranivima koja se koriste u hrani za
životinje, može prouzrokovati deficit ovog mikroelementa kod životinja. Putem namirnica animalnog porekla
simptomi deficita javljaju se i kod ljudi, čime se značajno slabi sistem antioksidativne zastite u organizmu.
Iskorisćavanje selena kod životinja zavisi od hemijskog oblika u kome se nalazi u obroku. Selen koji se koristi
kao dodatak u vitaminsko mineralnim predsmesama u hrani za koke nosilje prisutan je u jednom od dva
osnovna oblika: organski vezan za aminokiseline (selenocistein i selenometionin) ili u formi neorganske soli
(najčesće natrijum selenit). Nakon unosenja u organizam putem obroka, selen se ugrađuje u tkivne proteine
čime se stvara njegova rezerva. Deponovani selen u organizmu nalazi se u neaktivnom stanju i u slučajevima
oksidativnog stresa ili deficita selena u hrani, prelazi u aktivan oblik. Izvor selena u smesama za ishranu koka
nosilja ostvaruje uticaj na sadržaj selena u jajima. Dodatkom organskog selena nosiljama konzumnih jaja
mogu se postići količine od 20-25 µg po jajetu, sto je oko 30% od preporučenog dnevnog unosa za ljude. Za
proizvodnju takvih jaja potrebno je u hranu za nosilje dodati organskog selena u količini 0,3-0,5 mg/kg.
Organski izvori selena imaju bolju biolosku raspoloživost, a sadržaj selena u konzumnim jajima je stabilniji.
PB  - Kruševac : Asocijacija za kvalitet i standardizaciju Srbije
C3  - XXV Naučno stručni skup Sistem kvaliteta uslov za uspešno poslovanje i konkurentnost, Kopaonik, 17 - 19. maj 2023
T1  - Proizvodnja jaja obogaćenih selenom primenom biotehnoloških rešenja u ishrani koka nosilja
SP  - 68
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3013
ER  - 

@conference{
author = "Šefer, Dragan and Perić, Dejan and Jakić-Dimić, Dobrila",
year = "2023",
abstract = "Jaja predstavljaju važan izvor proteina, masti i mikroelemenata koji imaju značajnu ulogu u ishrani
ljudi. Proizvodnja i potrosnja jaja u svetu poslednjih decenija je u porastu. Konzumacija jaja dugo vremena
bila je vezana za negativne efekte na zdravlje ljudi, uglavnom zbog sadržaja holesterola. Međutim, danas je
poznato da na nivo holesterola u serumu utiče vise drugih faktora kao sto su genetska predispozicija,
hormonski status i hranidbene navike, a ne isključivo holesterol iz jaja. Poslednjih godina, namirnice koje se
svakodnevno koriste u ishrani ljudi nisu namenjene samo zadovoljenju potreba u osnovnim hranljivim
materijama nego se od hrane očekuje prevencija bolesti povezanih sa hranom i sticanje boljeg imunoloskog
statusa. Upotrebom specifičnih nutritivnih strategija moguće je proizvesti funkcionalnu hranu koja pored
osnovnih hranljivih materija sadrži i komponente koje učestvuju u očuvanju zdravlja i smanjenju rizika u
nastanku bolesti. Niska koncentracija selena u zemljistu, a posledično i u hranivima koja se koriste u hrani za
životinje, može prouzrokovati deficit ovog mikroelementa kod životinja. Putem namirnica animalnog porekla
simptomi deficita javljaju se i kod ljudi, čime se značajno slabi sistem antioksidativne zastite u organizmu.
Iskorisćavanje selena kod životinja zavisi od hemijskog oblika u kome se nalazi u obroku. Selen koji se koristi
kao dodatak u vitaminsko mineralnim predsmesama u hrani za koke nosilje prisutan je u jednom od dva
osnovna oblika: organski vezan za aminokiseline (selenocistein i selenometionin) ili u formi neorganske soli
(najčesće natrijum selenit). Nakon unosenja u organizam putem obroka, selen se ugrađuje u tkivne proteine
čime se stvara njegova rezerva. Deponovani selen u organizmu nalazi se u neaktivnom stanju i u slučajevima
oksidativnog stresa ili deficita selena u hrani, prelazi u aktivan oblik. Izvor selena u smesama za ishranu koka
nosilja ostvaruje uticaj na sadržaj selena u jajima. Dodatkom organskog selena nosiljama konzumnih jaja
mogu se postići količine od 20-25 µg po jajetu, sto je oko 30% od preporučenog dnevnog unosa za ljude. Za
proizvodnju takvih jaja potrebno je u hranu za nosilje dodati organskog selena u količini 0,3-0,5 mg/kg.
Organski izvori selena imaju bolju biolosku raspoloživost, a sadržaj selena u konzumnim jajima je stabilniji.",
publisher = "Kruševac : Asocijacija za kvalitet i standardizaciju Srbije",
journal = "XXV Naučno stručni skup Sistem kvaliteta uslov za uspešno poslovanje i konkurentnost, Kopaonik, 17 - 19. maj 2023",
title = "Proizvodnja jaja obogaćenih selenom primenom biotehnoloških rešenja u ishrani koka nosilja",
pages = "68-73",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3013"
}

Šefer, D., Perić, D.,& Jakić-Dimić, D.. (2023). Proizvodnja jaja obogaćenih selenom primenom biotehnoloških rešenja u ishrani koka nosilja. in XXV Naučno stručni skup Sistem kvaliteta uslov za uspešno poslovanje i konkurentnost, Kopaonik, 17 - 19. maj 2023
Kruševac : Asocijacija za kvalitet i standardizaciju Srbije., 68.
https://hdl.handle.net/21.15107/rcub_veterinar_3013

Šefer D, Perić D, Jakić-Dimić D. Proizvodnja jaja obogaćenih selenom primenom biotehnoloških rešenja u ishrani koka nosilja. in XXV Naučno stručni skup Sistem kvaliteta uslov za uspešno poslovanje i konkurentnost, Kopaonik, 17 - 19. maj 2023. 2023;:68.
https://hdl.handle.net/21.15107/rcub_veterinar_3013 .

Šefer, Dragan, Perić, Dejan, Jakić-Dimić, Dobrila, "Proizvodnja jaja obogaćenih selenom primenom biotehnoloških rešenja u ishrani koka nosilja" in XXV Naučno stručni skup Sistem kvaliteta uslov za uspešno poslovanje i konkurentnost, Kopaonik, 17 - 19. maj 2023 (2023):68,
https://hdl.handle.net/21.15107/rcub_veterinar_3013 .

TY  - CONF
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Kureljušić, Jasna
AU  - Rokvić, Nikola
AU  - Ninković, Milan
AU  - Savić Radovanović, Radoslava
AU  - Jakić-Dimić, Dobrila
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2765
AB  - Since the late years of the 20th century, listeriosis has been considered a
major public health concern. In the past years, Listeria monocytogenes has
been connected to more outbreaks of food-borne disease in humans with a
significant increasing trend in 2008. While it has been established that foodborne transmission constitutes the main acquisition route of listeriosis. Little
is known about L. monocytogenes antimicrobial resistance, especially for
non-human-origin isolates (Granier, 2011). Literature findings are that the most
of healthy humans are not significantly affected by the intake of L. monocytogenes in foods. The aim of the paper is to show an antimicrobial susceptibility
of L. monocytogenes isolates in the Veterinary Institute of Serbia in the last
2 years. In a total of 7 isolates of L. monocytogenes in general bacteriology
laboratory mainly origin from abortions of cattle, and others are from various
sources. Isolates have been isolated and identified following specific protocols
by selective broth enrichment and agar isolation procedure by OIE Manual of
Diagnostic Tests and Vaccines for Terrestrial Animals chapter 3.10.5 or similar
ISO 11290 standard for food chain isolates. Antimicrobial susceptibility has
been done by standard Kirby Bauer method ISO 20776. In general, all isolates
have been susceptible to penicillins (alone or with beta lactamase inhibitor),
tetracyclins, enrofloxacine and florfenicol. Some isolates showed resistance to
cephalosporins (28.57%), streptomycin (43.86%) and sulfonamides (14.29%).
 It seems that L. monocytogenes isolates from pathological processes in
animals and animal origin food still don’t exibit increased antimicrobial
insusceptibility in Serbia. Although Listeria spp. has been generally
reported susceptible to almost all antimicrobials, we still don’t agree
with foreign consideration that the emergence and spread of antimicrobial-resistant Listeria spp. has been attributed to the overuse of
antibiotics in disease treatments in domestic livestock.
AB  - Од касних година 20. века, листериоза се сматра главним проблемом јавног
здравља. Последњих година, Listeria monocitogenes је била повезана са више
избијања болести које се преносе храном код људи са значајним трендом
раста у 2008. Док је установљено да пренос путем хране представља главни
пут стицања листериозе. Мало се зна о антимикробној резистенцији L.
monocitogenes, посебно за изолате који нису људског порекла (Граниер,
2011). Литературни налази показују да већина здравих људи није значајно
погођена уносом L. monocitogenes у храну. Циљ рада је да се прикаже
антимикробна осетљивост изолата L. monocitogenes у Ветеринарском
институту Србије у последње 2 године. У укупно 7 изолата L. monocitogenes у
општој бактериолошкој лабораторији углавном потичу из абортуса говеда,
а остали су из различитих извора. Изолати су изоловани и идентификовани
према специфичним протоколима селективним обогаћивањем бујона и
процедуром изолације на агару према ОИЕ приручнику за дијагностичке
тестове и вакцине за копнене животиње, поглавље 3.10.5 или сличан
стандард ISO 11290 за изолате ланца исхране. Антимикробна осетљивост
је одређена стандардном Кирби Бауер методом ISO 20776. Генерално, сви
изолати су били осетљиви на пеницилине (сам или са инхибитором бета
лактамазе), тетрациклине, енрофлоксацин и флорфеникол. Неки изолати
су показали резистенцију на цефалоспорине (28,57%), стрептомицин
(43,86%) и сулфонамиде (14,29%).
Чини се да изолати L. monocitogenes из патолошких процеса у животињама и
храна животињског порекла још увек не показују повећану антимикробну
неосетљивост у Србији. Иако Listeria spp. је генерално пријављено да је
подложан скоро свим антимикробним средствима, наше искуство није у
корелацији мишљењем да је појава и ширење Listeria spp. се приписује
прекомерној употреби антибиотика у лечењу болести домаћих животиња.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022
T1  - Findings L. Monocytogenes and antimicrobial sensitivity during the period 2020-2022
T1  - Nalaz L. Monocytogenes i antimikrobna osetljivost tokom perioda 2020-2022
SP  - 324
EP  - 325
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2765
ER  - 

@conference{
author = "Zdravković, Nemanja and Radanović, Oliver and Kureljušić, Jasna and Rokvić, Nikola and Ninković, Milan and Savić Radovanović, Radoslava and Jakić-Dimić, Dobrila",
year = "2022",
abstract = "Since the late years of the 20th century, listeriosis has been considered a
major public health concern. In the past years, Listeria monocytogenes has
been connected to more outbreaks of food-borne disease in humans with a
significant increasing trend in 2008. While it has been established that foodborne transmission constitutes the main acquisition route of listeriosis. Little
is known about L. monocytogenes antimicrobial resistance, especially for
non-human-origin isolates (Granier, 2011). Literature findings are that the most
of healthy humans are not significantly affected by the intake of L. monocytogenes in foods. The aim of the paper is to show an antimicrobial susceptibility
of L. monocytogenes isolates in the Veterinary Institute of Serbia in the last
2 years. In a total of 7 isolates of L. monocytogenes in general bacteriology
laboratory mainly origin from abortions of cattle, and others are from various
sources. Isolates have been isolated and identified following specific protocols
by selective broth enrichment and agar isolation procedure by OIE Manual of
Diagnostic Tests and Vaccines for Terrestrial Animals chapter 3.10.5 or similar
ISO 11290 standard for food chain isolates. Antimicrobial susceptibility has
been done by standard Kirby Bauer method ISO 20776. In general, all isolates
have been susceptible to penicillins (alone or with beta lactamase inhibitor),
tetracyclins, enrofloxacine and florfenicol. Some isolates showed resistance to
cephalosporins (28.57%), streptomycin (43.86%) and sulfonamides (14.29%).
 It seems that L. monocytogenes isolates from pathological processes in
animals and animal origin food still don’t exibit increased antimicrobial
insusceptibility in Serbia. Although Listeria spp. has been generally
reported susceptible to almost all antimicrobials, we still don’t agree
with foreign consideration that the emergence and spread of antimicrobial-resistant Listeria spp. has been attributed to the overuse of
antibiotics in disease treatments in domestic livestock., Од касних година 20. века, листериоза се сматра главним проблемом јавног
здравља. Последњих година, Listeria monocitogenes је била повезана са више
избијања болести које се преносе храном код људи са значајним трендом
раста у 2008. Док је установљено да пренос путем хране представља главни
пут стицања листериозе. Мало се зна о антимикробној резистенцији L.
monocitogenes, посебно за изолате који нису људског порекла (Граниер,
2011). Литературни налази показују да већина здравих људи није значајно
погођена уносом L. monocitogenes у храну. Циљ рада је да се прикаже
антимикробна осетљивост изолата L. monocitogenes у Ветеринарском
институту Србије у последње 2 године. У укупно 7 изолата L. monocitogenes у
општој бактериолошкој лабораторији углавном потичу из абортуса говеда,
а остали су из различитих извора. Изолати су изоловани и идентификовани
према специфичним протоколима селективним обогаћивањем бујона и
процедуром изолације на агару према ОИЕ приручнику за дијагностичке
тестове и вакцине за копнене животиње, поглавље 3.10.5 или сличан
стандард ISO 11290 за изолате ланца исхране. Антимикробна осетљивост
је одређена стандардном Кирби Бауер методом ISO 20776. Генерално, сви
изолати су били осетљиви на пеницилине (сам или са инхибитором бета
лактамазе), тетрациклине, енрофлоксацин и флорфеникол. Неки изолати
су показали резистенцију на цефалоспорине (28,57%), стрептомицин
(43,86%) и сулфонамиде (14,29%).
Чини се да изолати L. monocitogenes из патолошких процеса у животињама и
храна животињског порекла још увек не показују повећану антимикробну
неосетљивост у Србији. Иако Listeria spp. је генерално пријављено да је
подложан скоро свим антимикробним средствима, наше искуство није у
корелацији мишљењем да је појава и ширење Listeria spp. се приписује
прекомерној употреби антибиотика у лечењу болести домаћих животиња.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022",
title = "Findings L. Monocytogenes and antimicrobial sensitivity during the period 2020-2022, Nalaz L. Monocytogenes i antimikrobna osetljivost tokom perioda 2020-2022",
pages = "324-325",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2765"
}

Zdravković, N., Radanović, O., Kureljušić, J., Rokvić, N., Ninković, M., Savić Radovanović, R.,& Jakić-Dimić, D.. (2022). Findings L. Monocytogenes and antimicrobial sensitivity during the period 2020-2022. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022
Banja Luka : Veterinarska komora Republike Srpske., 324-325.
https://hdl.handle.net/21.15107/rcub_veterinar_2765

Zdravković N, Radanović O, Kureljušić J, Rokvić N, Ninković M, Savić Radovanović R, Jakić-Dimić D. Findings L. Monocytogenes and antimicrobial sensitivity during the period 2020-2022. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022. 2022;:324-325.
https://hdl.handle.net/21.15107/rcub_veterinar_2765 .

Zdravković, Nemanja, Radanović, Oliver, Kureljušić, Jasna, Rokvić, Nikola, Ninković, Milan, Savić Radovanović, Radoslava, Jakić-Dimić, Dobrila, "Findings L. Monocytogenes and antimicrobial sensitivity during the period 2020-2022" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022 (2022):324-325,
https://hdl.handle.net/21.15107/rcub_veterinar_2765 .

TY  - CONF
AU  - Marković, Radmila
AU  - Baltić, Milan Ž.
AU  - Radulović, Stamen
AU  - Šefer, Dragan
AU  - Jakić-Dimić, Dobrila
AU  - Glišić, Milica
AU  - Pavlović, Marija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2992
AB  - Soja (Glycine max) je biljna vrsta koja pripada mahunarkama. Ona je jedan od najvećih i najefikasnijih izvora biljnih proteina sa bogatim i uravnoteženim profilom amino kiselina, ali i značajan izvor ulja. Visoko vrednim hranivima u ishrani životinja se smatraju i proizvodi od soje (griz, sačma, pogača). Pored visokoverdnih sastojaka, zrno soje sadrži i materije (kao što su inhibitori tripsina i himotripsina, enzim ureaza itd.) koje smanjuju biološku vrednost ovog hraniva pa je neophodna termička obrada ovog hraniva. Veća potražnja za sojom je iziskivala genetske modifikacije zbog uticaja na prinos, sorte otporne na bolest, varijetete sa pojačanom hranljivom vrednošću kao i sorte tolerantne sušu. Česte su i alergijske reakcije na soju.
AB  - Soybean ( ) is a plant species belonging to the legumes. It is one of the largest and Glycine max
most ef6icient sources of vegetable protein with a rich and balanced amino acid pro6ile, but
also a signi6icant source of oil. Highly valuable nutrients in animal nutrition are considered
and soy products (full-fat soy meal, meal, cake). In addition to the high value ingredients,
soybean contains a substance (such as inhibitors of trypsin and chymotrypsin, the enzyme
urease, etc.) That reduce the biological value of the feed is required thermal treatment of
the feed. Increasing demand for soybean genetic modi6ication is necessitated due to the
impact on yield, disease-resistant varieties, varieties with enhanced nutritional value as
well as the varieties tolerant to drought. Often there are allergic reactions to soy.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 21. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 8 - 11. jun, 2016
T1  - Soja u ishrani monogastričnih životinja
T1  - Soya in monogastric animals nutrition
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2992
ER  - 

@conference{
author = "Marković, Radmila and Baltić, Milan Ž. and Radulović, Stamen and Šefer, Dragan and Jakić-Dimić, Dobrila and Glišić, Milica and Pavlović, Marija",
year = "2016",
abstract = "Soja (Glycine max) je biljna vrsta koja pripada mahunarkama. Ona je jedan od najvećih i najefikasnijih izvora biljnih proteina sa bogatim i uravnoteženim profilom amino kiselina, ali i značajan izvor ulja. Visoko vrednim hranivima u ishrani životinja se smatraju i proizvodi od soje (griz, sačma, pogača). Pored visokoverdnih sastojaka, zrno soje sadrži i materije (kao što su inhibitori tripsina i himotripsina, enzim ureaza itd.) koje smanjuju biološku vrednost ovog hraniva pa je neophodna termička obrada ovog hraniva. Veća potražnja za sojom je iziskivala genetske modifikacije zbog uticaja na prinos, sorte otporne na bolest, varijetete sa pojačanom hranljivom vrednošću kao i sorte tolerantne sušu. Česte su i alergijske reakcije na soju., Soybean ( ) is a plant species belonging to the legumes. It is one of the largest and Glycine max
most ef6icient sources of vegetable protein with a rich and balanced amino acid pro6ile, but
also a signi6icant source of oil. Highly valuable nutrients in animal nutrition are considered
and soy products (full-fat soy meal, meal, cake). In addition to the high value ingredients,
soybean contains a substance (such as inhibitors of trypsin and chymotrypsin, the enzyme
urease, etc.) That reduce the biological value of the feed is required thermal treatment of
the feed. Increasing demand for soybean genetic modi6ication is necessitated due to the
impact on yield, disease-resistant varieties, varieties with enhanced nutritional value as
well as the varieties tolerant to drought. Often there are allergic reactions to soy.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "21. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 8 - 11. jun, 2016",
title = "Soja u ishrani monogastričnih životinja, Soya in monogastric animals nutrition",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2992"
}

Marković, R., Baltić, M. Ž., Radulović, S., Šefer, D., Jakić-Dimić, D., Glišić, M.,& Pavlović, M.. (2016). Soja u ishrani monogastričnih životinja. in 21. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 8 - 11. jun, 2016
Banja Luka : Veterinarska komora Republike Srpske., 31-32.
https://hdl.handle.net/21.15107/rcub_veterinar_2992

Marković R, Baltić MŽ, Radulović S, Šefer D, Jakić-Dimić D, Glišić M, Pavlović M. Soja u ishrani monogastričnih životinja. in 21. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 8 - 11. jun, 2016. 2016;:31-32.
https://hdl.handle.net/21.15107/rcub_veterinar_2992 .

Marković, Radmila, Baltić, Milan Ž., Radulović, Stamen, Šefer, Dragan, Jakić-Dimić, Dobrila, Glišić, Milica, Pavlović, Marija, "Soja u ishrani monogastričnih životinja" in 21. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 8 - 11. jun, 2016 (2016):31-32,
https://hdl.handle.net/21.15107/rcub_veterinar_2992 .

TY  - JOUR
AU  - Cvetojević, D.
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Jakić-Dimić, Dobrila
AU  - Pavlović, Miloš
AU  - Spalević, Ljiljana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1416
AB  - Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. In this study a retrospective PCR examination of 100 extracted DNA samples from aborting cows was performed in order to determine: prevalence of BHV-4 in abortive cattle, whether coinfections BHV-4 with other abortifacient pathogens are present in the same sample and to determine the month of gestation when BHV-4 associated abortions were detected. Out of 100 examined samples, the BHV-4 genome was detected in 21 samples (21%). In two samples we detected coinfection of BHV-4 with bovine viral diarrhea virus (BVDV) and in one with Neospora caninum. Most of the BHV-4-associated abortions were detected during the seventh month of gestation. It was concluded that an active BHV-4 infection was present among cows that aborted on the farms examined. The high prevalence of the BHV-4 genome in abortion material suggests that this virus may have cause the abortions. Further studies and examinations are needed to establish causative connection between presence of BHV-4 and abortion.
PB  - Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa
T2  - Polish Journal of Veterinary Sciences
T1  - Prevalence of Bovine herpesvirus type 4 in aborting dairy cows
VL  - 19
IS  - 4
SP  - 731
EP  - 736
DO  - 10.1515/pjvs-2016-0092
ER  - 

@article{
author = "Cvetojević, D. and Savić, Božidar and Milićević, Vesna and Kureljušić, Branislav and Jezdimirović, Nemanja and Jakić-Dimić, Dobrila and Pavlović, Miloš and Spalević, Ljiljana",
year = "2016",
abstract = "Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. In this study a retrospective PCR examination of 100 extracted DNA samples from aborting cows was performed in order to determine: prevalence of BHV-4 in abortive cattle, whether coinfections BHV-4 with other abortifacient pathogens are present in the same sample and to determine the month of gestation when BHV-4 associated abortions were detected. Out of 100 examined samples, the BHV-4 genome was detected in 21 samples (21%). In two samples we detected coinfection of BHV-4 with bovine viral diarrhea virus (BVDV) and in one with Neospora caninum. Most of the BHV-4-associated abortions were detected during the seventh month of gestation. It was concluded that an active BHV-4 infection was present among cows that aborted on the farms examined. The high prevalence of the BHV-4 genome in abortion material suggests that this virus may have cause the abortions. Further studies and examinations are needed to establish causative connection between presence of BHV-4 and abortion.",
publisher = "Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa",
journal = "Polish Journal of Veterinary Sciences",
title = "Prevalence of Bovine herpesvirus type 4 in aborting dairy cows",
volume = "19",
number = "4",
pages = "731-736",
doi = "10.1515/pjvs-2016-0092"
}

Cvetojević, D., Savić, B., Milićević, V., Kureljušić, B., Jezdimirović, N., Jakić-Dimić, D., Pavlović, M.,& Spalević, L.. (2016). Prevalence of Bovine herpesvirus type 4 in aborting dairy cows. in Polish Journal of Veterinary Sciences
Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa., 19(4), 731-736.
https://doi.org/10.1515/pjvs-2016-0092

Cvetojević D, Savić B, Milićević V, Kureljušić B, Jezdimirović N, Jakić-Dimić D, Pavlović M, Spalević L. Prevalence of Bovine herpesvirus type 4 in aborting dairy cows. in Polish Journal of Veterinary Sciences. 2016;19(4):731-736.
doi:10.1515/pjvs-2016-0092 .

Cvetojević, D., Savić, Božidar, Milićević, Vesna, Kureljušić, Branislav, Jezdimirović, Nemanja, Jakić-Dimić, Dobrila, Pavlović, Miloš, Spalević, Ljiljana, "Prevalence of Bovine herpesvirus type 4 in aborting dairy cows" in Polish Journal of Veterinary Sciences, 19, no. 4 (2016):731-736,
https://doi.org/10.1515/pjvs-2016-0092 . .

TY  - JOUR
AU  - Marković, Radmila
AU  - Radulović, Stamen
AU  - Teodorović, Vlado
AU  - Petrujkić, Branko
AU  - Jakić-Dimić, Dobrila
AU  - Peurača, Mile
AU  - Šefer, Dragan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2126
AB  - Intenziviranje stočarske proizvodnje dovelo je do toga da reprodukcija, kao primarna
fiziološka funkcija svakog živog organizma sa ciljem produženja vrste, u odnosu
na biološke zakone i potrebe bude višestruko ugrožena, odnosno rezultira evidentno
slabijim rezultatima.
Ispoljavanje genetskog potencijala za proizvodnju životinja je u neposrednoj zavisnosti
od ishrane. Nepravilna i deficitarna ishrana, pored toga što dovodi do smanjenja
obima proizvodnje (mesa, mleka, jaja), izuzetno nepovoljno se odražava i na uspeh
reprodukcije. Nepovoljan uticaj ishrane se manifestuje poremećajem ili izostankom
estrusa, smanjenom koncepcijom, resorpcijom ploda ili pobačajem, rađanjem avitalnih
ili mrtvih mladunaca itd. Nasuprot navedenom, pravilna ishrana reproduktivnih
grla, čiji organizam treba da podnese sve napore višegodišnje intenzivne proizvodnje,
omogućava njihovo duže zadržavanje i eksploataciju u proizvodnji.
Plodnost životinja predstavlja jedan od osnovnih faktora ekonomičnosti proizvodnog
procesa. Među brojnim faktorima koji utiču na nastanak poremećaja reprodukcije
i plodnosti, po svom značaju izdvajaju se nutritivni faktori (energetska vrednost
obroka, sadržaj proteina, mineralnih materija i vitamina). Kroz brojne oglede ishrane
utvrđeno je da, kako nedovoljna količina, tako i veća količina hranljivih materija može
u velikoj meri da utiče na proces reprodukcije. Osnovni problemi su vezani za stepen
suficita, deficita ili disbalansa u ishrani reproduktivnih domaćih životinja. Pored toga,
u hrani za životinje mogu da se nađu i štetne materije (estrogen biljnog porekla, ili sekundarni
metaboliti plesni) koje mogu u značajnoj meri da naruše reproduktivni ciklus
u svim njegovim fazama. Najbolja preporuka je da se životinjama obezbedi izbalansiran obrok u svim hranljivim sastojcima koji zadovoljavaju prethodno normirane nutritivne zahteve i preporuke.
AB  - Intensification of livestock production has led to the fact that the reproduction, as
the primary physiological function of every living organism with the aim of extending
the species, in relation to biological laws and requirements became multiple compromised,
or to result in evidently lower performance.
The manifestation of genetic potential for animal production is highly dependent
on nutrition. Irregular and deficient diet, in addition of leading to a reduction in the
volume of production (meat, milk, eggs) extremely adversely affects the success of reproduction.
The adverse effects of eating disorder is manifested or absence of estrus,
reduced conception, fetal resorption or abortion, giving birth to non-vital or dead
cubs etc. In contrast to stated, the proper nutrition of reproductive animals whose organism
should endure the difficulties of many years of intensive production enables
their longer retention and utilization in manufacturing.
Fertility represents also a basic factor of economical production. Among many different
factors that affect fertility and reproductive disorders by its significant stand
out nutritional factors energy value of diet, content of protein, minerals and vitamins.
Through the many feeding trials, it has been documented that insufficient quantity as
well as a greater amount of nutrients can significantly affect the process of reproduction.
The main problems are related to the degree of excess, deficit or imbalance of
nutrients in the diet of domestic reproductive animals. In addition, the feed can contain
harmful substances (estrogens of plant origin, or secondary metabolites of molds)
that can significantly impair the reproductive cycle in all its phases. The best recommendation
is to provide to animals a completely balanced meal in all nutrients that
meet previously established nutritional requirements and recommendations.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Nutritivni faktori kao uzroci poremećaja u reprodukciji domaćih životinja
T1  - Nutritional factors as causes of reproductive disorders of domestic animals
VL  - 15
IS  - 2
SP  - 233
EP  - 250
DO  - 10.7251/VETJ1502233M
ER  - 

@article{
author = "Marković, Radmila and Radulović, Stamen and Teodorović, Vlado and Petrujkić, Branko and Jakić-Dimić, Dobrila and Peurača, Mile and Šefer, Dragan",
year = "2015",
abstract = "Intenziviranje stočarske proizvodnje dovelo je do toga da reprodukcija, kao primarna
fiziološka funkcija svakog živog organizma sa ciljem produženja vrste, u odnosu
na biološke zakone i potrebe bude višestruko ugrožena, odnosno rezultira evidentno
slabijim rezultatima.
Ispoljavanje genetskog potencijala za proizvodnju životinja je u neposrednoj zavisnosti
od ishrane. Nepravilna i deficitarna ishrana, pored toga što dovodi do smanjenja
obima proizvodnje (mesa, mleka, jaja), izuzetno nepovoljno se odražava i na uspeh
reprodukcije. Nepovoljan uticaj ishrane se manifestuje poremećajem ili izostankom
estrusa, smanjenom koncepcijom, resorpcijom ploda ili pobačajem, rađanjem avitalnih
ili mrtvih mladunaca itd. Nasuprot navedenom, pravilna ishrana reproduktivnih
grla, čiji organizam treba da podnese sve napore višegodišnje intenzivne proizvodnje,
omogućava njihovo duže zadržavanje i eksploataciju u proizvodnji.
Plodnost životinja predstavlja jedan od osnovnih faktora ekonomičnosti proizvodnog
procesa. Među brojnim faktorima koji utiču na nastanak poremećaja reprodukcije
i plodnosti, po svom značaju izdvajaju se nutritivni faktori (energetska vrednost
obroka, sadržaj proteina, mineralnih materija i vitamina). Kroz brojne oglede ishrane
utvrđeno je da, kako nedovoljna količina, tako i veća količina hranljivih materija može
u velikoj meri da utiče na proces reprodukcije. Osnovni problemi su vezani za stepen
suficita, deficita ili disbalansa u ishrani reproduktivnih domaćih životinja. Pored toga,
u hrani za životinje mogu da se nađu i štetne materije (estrogen biljnog porekla, ili sekundarni
metaboliti plesni) koje mogu u značajnoj meri da naruše reproduktivni ciklus
u svim njegovim fazama. Najbolja preporuka je da se životinjama obezbedi izbalansiran obrok u svim hranljivim sastojcima koji zadovoljavaju prethodno normirane nutritivne zahteve i preporuke., Intensification of livestock production has led to the fact that the reproduction, as
the primary physiological function of every living organism with the aim of extending
the species, in relation to biological laws and requirements became multiple compromised,
or to result in evidently lower performance.
The manifestation of genetic potential for animal production is highly dependent
on nutrition. Irregular and deficient diet, in addition of leading to a reduction in the
volume of production (meat, milk, eggs) extremely adversely affects the success of reproduction.
The adverse effects of eating disorder is manifested or absence of estrus,
reduced conception, fetal resorption or abortion, giving birth to non-vital or dead
cubs etc. In contrast to stated, the proper nutrition of reproductive animals whose organism
should endure the difficulties of many years of intensive production enables
their longer retention and utilization in manufacturing.
Fertility represents also a basic factor of economical production. Among many different
factors that affect fertility and reproductive disorders by its significant stand
out nutritional factors energy value of diet, content of protein, minerals and vitamins.
Through the many feeding trials, it has been documented that insufficient quantity as
well as a greater amount of nutrients can significantly affect the process of reproduction.
The main problems are related to the degree of excess, deficit or imbalance of
nutrients in the diet of domestic reproductive animals. In addition, the feed can contain
harmful substances (estrogens of plant origin, or secondary metabolites of molds)
that can significantly impair the reproductive cycle in all its phases. The best recommendation
is to provide to animals a completely balanced meal in all nutrients that
meet previously established nutritional requirements and recommendations.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Nutritivni faktori kao uzroci poremećaja u reprodukciji domaćih životinja, Nutritional factors as causes of reproductive disorders of domestic animals",
volume = "15",
number = "2",
pages = "233-250",
doi = "10.7251/VETJ1502233M"
}

Marković, R., Radulović, S., Teodorović, V., Petrujkić, B., Jakić-Dimić, D., Peurača, M.,& Šefer, D.. (2015). Nutritivni faktori kao uzroci poremećaja u reprodukciji domaćih životinja. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 15(2), 233-250.
https://doi.org/10.7251/VETJ1502233M

Marković R, Radulović S, Teodorović V, Petrujkić B, Jakić-Dimić D, Peurača M, Šefer D. Nutritivni faktori kao uzroci poremećaja u reprodukciji domaćih životinja. in Veterinarski žurnal Republike Srpske. 2015;15(2):233-250.
doi:10.7251/VETJ1502233M .

Marković, Radmila, Radulović, Stamen, Teodorović, Vlado, Petrujkić, Branko, Jakić-Dimić, Dobrila, Peurača, Mile, Šefer, Dragan, "Nutritivni faktori kao uzroci poremećaja u reprodukciji domaćih životinja" in Veterinarski žurnal Republike Srpske, 15, no. 2 (2015):233-250,
https://doi.org/10.7251/VETJ1502233M . .

TY  - CONF
AU  - Petrujkić, Branko
AU  - Jovanović, Dragoljub
AU  - Marković, Radmila
AU  - Janjić, Jelena
AU  - Jakić-Dimić, Dobrila
AU  - Teodorović, Vlado
AU  - Šefer, Dragan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2672
PB  - European Association of Fish Pathologists
C3  - 17th International Conference on diseases of fish and shell-fish. Las Palmas Gran Canaria, September 7-11,2015/Bulletin of the European Association of Fish Pathologists (EAFP Bulletin)
T1  - Monitoring of environmental heavy metals accumulation in fish muscles from the two lakes in Mladenovac municipality, Belgrade area, Serbia
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2672
ER  - 

@conference{
author = "Petrujkić, Branko and Jovanović, Dragoljub and Marković, Radmila and Janjić, Jelena and Jakić-Dimić, Dobrila and Teodorović, Vlado and Šefer, Dragan",
year = "2015",
publisher = "European Association of Fish Pathologists",
journal = "17th International Conference on diseases of fish and shell-fish. Las Palmas Gran Canaria, September 7-11,2015/Bulletin of the European Association of Fish Pathologists (EAFP Bulletin)",
title = "Monitoring of environmental heavy metals accumulation in fish muscles from the two lakes in Mladenovac municipality, Belgrade area, Serbia",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2672"
}

Petrujkić, B., Jovanović, D., Marković, R., Janjić, J., Jakić-Dimić, D., Teodorović, V.,& Šefer, D.. (2015). Monitoring of environmental heavy metals accumulation in fish muscles from the two lakes in Mladenovac municipality, Belgrade area, Serbia. in 17th International Conference on diseases of fish and shell-fish. Las Palmas Gran Canaria, September 7-11,2015/Bulletin of the European Association of Fish Pathologists (EAFP Bulletin)
European Association of Fish Pathologists., 48-48.
https://hdl.handle.net/21.15107/rcub_veterinar_2672

Petrujkić B, Jovanović D, Marković R, Janjić J, Jakić-Dimić D, Teodorović V, Šefer D. Monitoring of environmental heavy metals accumulation in fish muscles from the two lakes in Mladenovac municipality, Belgrade area, Serbia. in 17th International Conference on diseases of fish and shell-fish. Las Palmas Gran Canaria, September 7-11,2015/Bulletin of the European Association of Fish Pathologists (EAFP Bulletin). 2015;:48-48.
https://hdl.handle.net/21.15107/rcub_veterinar_2672 .

Petrujkić, Branko, Jovanović, Dragoljub, Marković, Radmila, Janjić, Jelena, Jakić-Dimić, Dobrila, Teodorović, Vlado, Šefer, Dragan, "Monitoring of environmental heavy metals accumulation in fish muscles from the two lakes in Mladenovac municipality, Belgrade area, Serbia" in 17th International Conference on diseases of fish and shell-fish. Las Palmas Gran Canaria, September 7-11,2015/Bulletin of the European Association of Fish Pathologists (EAFP Bulletin) (2015):48-48,
https://hdl.handle.net/21.15107/rcub_veterinar_2672 .

TY  - JOUR
AU  - Radulović, Stamen
AU  - Marković, Radmila
AU  - Jakić-Dimić, Dobrila
AU  - Šefer, Dragan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1201
AB  - Phytogene feed additives (phytobiotics) are plant origin compounds used in animal nutrition in order to improve their productivity by improving the production performance of animals, feed properties and the quality of food of animal origin. These additives stimulate animal growth by using their natural and physiological potentials and mechanisms providing conditions for the realization of the genetically projected production volumes. Data on the use of phytobiotics as growth stimulators are incomplete and often very contradictory, especially those related to their impact on the health and performance of weaned pigs. Given the relevance and importance of these issues the feeding trial was organized by the group-control system. The experiment lasted for 40 days and it was divided into two phases of 20 days each. Trial was conducted on 24 piglets, F1 generation of Swedish Landrace and Pietrain, weaned from sows at the age of 35 days. Tests were carried out on piglets, with an average body weight of 8.61±1.59 kg, which were subjected to one of the two feeding treatments immediately after weaning. The control group was fed with a mixture without growth promoters, while the experimental group received a diet containing preparation of phytobiotic (Enviva EO 101, Danisco Animal Nutrition) in the amount recommended by the manufacturer (0.1 kg/t). The mixtures for piglets nutrition were formulated in accordance with the recommendations of the NRC (1998), and AEC (1993) and they met the nutritional requirements completely. During the experiment, there was no disturbance of health and/or the manifestation of clinical signs of disease. The control group, fed with the diet without added growth stimulators, achieved body weight (25.32±6.31 kg), average daily gain (0.42±0.12 kg), consumption (0.89 kg) and feed conversion (2.119 ) normal for a given race, age and housing conditions. The use of preparation of phytobiotics as growth promoters, led to better production results in regard to the control group, which was based on higher body weight (27.19±4.77 kg), higher average daily gain (0.46±0.09 kg) and better feed conversion (2.043). The use of phytobiotic, as an alternative option in growth stimulation of weaned pigs, has its nutritive, medical and economic justification.
AB  - Fitogeni dodaci hrani za životinje (fitobiotici) predstavljaju jedinjenja biljnog porekla koja se koriste u ishrani životinja za unapređenje njihove produktivnosti putem poboljšanja proizvodnih rezultata, svojstava hrane, kao i kvaliteta namirnica animalnog porekla. Navedeni dodaci omogućavaju stimulaciju rasta životinja korišćenjem njihovih prirodnih fizioloških potencijala i mehanizama, obezbeđujući uslove za ostvarenje genetski projektovanog obima proizvodnje. Podaci o upotrebi fitobiotika kao stimulatora rasta su nepotpuni i često vrlo kontradiktorni, naročito oni koji se odnose na njihov uticaj na zdravstveno stanje i proizvodne rezultate. S obzirom na aktuelnost i značaj navedene problematike organizovan je ogled ishrane po grupno-kontrolnom sistemu. Ogled je trajao 40 dana, a podeljen je u dve faze od po 20 dana. Ogledom je izveden na 24 praseta, melezi švedskog landrasa i pietrena, odbijena od krmače u starosti od 35 dana. Ispitivanja su izvedena na prasadima oba pola, prosečne telesne mase 8,61±1,59 kg koja su odmah nakon odbijanja raspoređena u jedan od dva hranidbena tretmana. Kontrolna grupa prasadi hranjena je smešom bez stimulatora rasta, dok je ogledna grupa dobijala hranu sa dodatkom preparata fitobiotika (Enviva EO 101, Danisco Animal Nutrition) u količini preporučenoj od strane proizvođača (0,1 kg/t). Smeše za ishranu prasadi su bile formulisane u skladu sa preporukama NRC (1998), kao i AEC (1993) i u potpunosti su odgovarale njihovim nutritivnim zahtevima. Tokom ogleda nije došlo do poremećaja zdravstvenog stanja i/ ili ispoljavanja kliničkih znakova oboljenja. Kontrolna grupa prasadi hranjena smešama bez dodatog stimulatora rasta postigla je telesnu masu (25,32±6,31 kg), dnevni prirast (0,42±0,12 kg), konzumaciju (0,89 kg) i konverziju hrane (2,119) uobičajenu za datu rasu, starost I uslove držanja. Korišćenjem preparata fitobiotika kao stimulatora rasta postignuti su bolji proizvodni rezultati u odnosu na kontrolnu grupu, zasnovani na većoj postignutoj telesnoj masi (27,19±4,77kg), većem ostvarenom prosečnom dnevnom prirastu (0,46±0,09 kg) i boljoj konverziji hrane (2,043). Korišćenje fitobiotika kao alternativne mogućnosti u stimulaciji rasta prasadi u odgoju ima svoje nutritivno, medicinsko i ekonomsko opravdanje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Upotreba fitobiotika u stimulaciji rasta odbijene prasadi
VL  - 69
IS  - 1-2
SP  - 63
EP  - 74
DO  - 10.2298/VETGL1502063R
ER  - 

@article{
author = "Radulović, Stamen and Marković, Radmila and Jakić-Dimić, Dobrila and Šefer, Dragan",
year = "2015",
abstract = "Phytogene feed additives (phytobiotics) are plant origin compounds used in animal nutrition in order to improve their productivity by improving the production performance of animals, feed properties and the quality of food of animal origin. These additives stimulate animal growth by using their natural and physiological potentials and mechanisms providing conditions for the realization of the genetically projected production volumes. Data on the use of phytobiotics as growth stimulators are incomplete and often very contradictory, especially those related to their impact on the health and performance of weaned pigs. Given the relevance and importance of these issues the feeding trial was organized by the group-control system. The experiment lasted for 40 days and it was divided into two phases of 20 days each. Trial was conducted on 24 piglets, F1 generation of Swedish Landrace and Pietrain, weaned from sows at the age of 35 days. Tests were carried out on piglets, with an average body weight of 8.61±1.59 kg, which were subjected to one of the two feeding treatments immediately after weaning. The control group was fed with a mixture without growth promoters, while the experimental group received a diet containing preparation of phytobiotic (Enviva EO 101, Danisco Animal Nutrition) in the amount recommended by the manufacturer (0.1 kg/t). The mixtures for piglets nutrition were formulated in accordance with the recommendations of the NRC (1998), and AEC (1993) and they met the nutritional requirements completely. During the experiment, there was no disturbance of health and/or the manifestation of clinical signs of disease. The control group, fed with the diet without added growth stimulators, achieved body weight (25.32±6.31 kg), average daily gain (0.42±0.12 kg), consumption (0.89 kg) and feed conversion (2.119 ) normal for a given race, age and housing conditions. The use of preparation of phytobiotics as growth promoters, led to better production results in regard to the control group, which was based on higher body weight (27.19±4.77 kg), higher average daily gain (0.46±0.09 kg) and better feed conversion (2.043). The use of phytobiotic, as an alternative option in growth stimulation of weaned pigs, has its nutritive, medical and economic justification., Fitogeni dodaci hrani za životinje (fitobiotici) predstavljaju jedinjenja biljnog porekla koja se koriste u ishrani životinja za unapređenje njihove produktivnosti putem poboljšanja proizvodnih rezultata, svojstava hrane, kao i kvaliteta namirnica animalnog porekla. Navedeni dodaci omogućavaju stimulaciju rasta životinja korišćenjem njihovih prirodnih fizioloških potencijala i mehanizama, obezbeđujući uslove za ostvarenje genetski projektovanog obima proizvodnje. Podaci o upotrebi fitobiotika kao stimulatora rasta su nepotpuni i često vrlo kontradiktorni, naročito oni koji se odnose na njihov uticaj na zdravstveno stanje i proizvodne rezultate. S obzirom na aktuelnost i značaj navedene problematike organizovan je ogled ishrane po grupno-kontrolnom sistemu. Ogled je trajao 40 dana, a podeljen je u dve faze od po 20 dana. Ogledom je izveden na 24 praseta, melezi švedskog landrasa i pietrena, odbijena od krmače u starosti od 35 dana. Ispitivanja su izvedena na prasadima oba pola, prosečne telesne mase 8,61±1,59 kg koja su odmah nakon odbijanja raspoređena u jedan od dva hranidbena tretmana. Kontrolna grupa prasadi hranjena je smešom bez stimulatora rasta, dok je ogledna grupa dobijala hranu sa dodatkom preparata fitobiotika (Enviva EO 101, Danisco Animal Nutrition) u količini preporučenoj od strane proizvođača (0,1 kg/t). Smeše za ishranu prasadi su bile formulisane u skladu sa preporukama NRC (1998), kao i AEC (1993) i u potpunosti su odgovarale njihovim nutritivnim zahtevima. Tokom ogleda nije došlo do poremećaja zdravstvenog stanja i/ ili ispoljavanja kliničkih znakova oboljenja. Kontrolna grupa prasadi hranjena smešama bez dodatog stimulatora rasta postigla je telesnu masu (25,32±6,31 kg), dnevni prirast (0,42±0,12 kg), konzumaciju (0,89 kg) i konverziju hrane (2,119) uobičajenu za datu rasu, starost I uslove držanja. Korišćenjem preparata fitobiotika kao stimulatora rasta postignuti su bolji proizvodni rezultati u odnosu na kontrolnu grupu, zasnovani na većoj postignutoj telesnoj masi (27,19±4,77kg), većem ostvarenom prosečnom dnevnom prirastu (0,46±0,09 kg) i boljoj konverziji hrane (2,043). Korišćenje fitobiotika kao alternativne mogućnosti u stimulaciji rasta prasadi u odgoju ima svoje nutritivno, medicinsko i ekonomsko opravdanje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Upotreba fitobiotika u stimulaciji rasta odbijene prasadi",
volume = "69",
number = "1-2",
pages = "63-74",
doi = "10.2298/VETGL1502063R"
}

Radulović, S., Marković, R., Jakić-Dimić, D.,& Šefer, D.. (2015). Upotreba fitobiotika u stimulaciji rasta odbijene prasadi. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 63-74.
https://doi.org/10.2298/VETGL1502063R

Radulović S, Marković R, Jakić-Dimić D, Šefer D. Upotreba fitobiotika u stimulaciji rasta odbijene prasadi. in Veterinarski Glasnik. 2015;69(1-2):63-74.
doi:10.2298/VETGL1502063R .

Radulović, Stamen, Marković, Radmila, Jakić-Dimić, Dobrila, Šefer, Dragan, "Upotreba fitobiotika u stimulaciji rasta odbijene prasadi" in Veterinarski Glasnik, 69, no. 1-2 (2015):63-74,
https://doi.org/10.2298/VETGL1502063R . .

TY  - JOUR
AU  - Cvetojević, Đorđe
AU  - Savić, Božidar
AU  - Stanojević, Slobodan
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Bojković-Kovačević, Slavica
AU  - Pavlović, Miloš
AU  - Katić, Marko
AU  - Jakić-Dimić, Dobrila
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1298
AB  - Abortions represents significant problem in modern cattle husbandry because they directly and indirectly affects profit from production. Abortions in cattle can be caused by infectious (viruses, bacteria, protozoa, fungi) or non-infectious agents. Infectious agents can have beside their abortive effect other organ pathology which additionally compromise health of animal and also production. Also, some causative agents are zoonotic. This paper represents results from diagnostic examination of samples from cows which aborted on PKB farms during 2014. By polymerase chain reaction (PCR), we investigated presence of genom of BVD virus, IBR virus, Schmallenberg virus, Neospora caninum and Coxiella burnetii. Samples (fetus, placenta, swabs) from 137 cows which aborted were examined and 46 cows (33,5%) were positive on some of causative agents. Presence of one of pathogens was detected in 43 cows (31,4%o), while samples from 3 cows (2,2%o) were positive for two pathogens simultaneously. According to this results, the most common agents were Neospora caninum and BVD virus which were detected in 23 (16,8%) and 19 (13,8%) cows, respectively. Coxiella burnetii was proven in 6 cows (4,4%), IBR virus in one, while presence of Schmallenberg virus was not detected in any cow. Current results suggests that contagious abortive agents are present significantly on PKB corporation farms. Tight cooperation between veterinary service and diagnostic laboratory is necessary for their control and detection.
AB  - Abortusi predstavljaju značajan problem u savremenom uzgoju goveda jer direktno i indirektno utiču na ekonomsku isplativost proizvodnje. Pobačaji goveda mogu biti izazvani infektivnim (virusi, bakterije, protozoe, gljivice) ili neinfektivnim faktorima. Infektivni agensi kao uzročnici pobačaja mogu pored abortogenog efekta imati i drugu organopatologiju čime dodatno ugrožavaju zdravstveno stanje životinja, a time i proizvodnju. Takođe, pojedini uzročnici imaju i zoonotski potencijal. U ovom radu prikazani su rezultati dijagnostičkih ispitivanja uzoraka poreklom od krava koje su abortirale sa gazdinstava PKB-a tokom 2014. godine. Metodom reakcije lančane polimeraze (PCR) ispitivano je prisustvo genoma BVD virusa, IBR virusa, Šmalenberg virusa, Neospora caninum i Coxiella burnetii. Pregledani su uzorci (fetus, placenta ili brisevi) od 137 krava koje su abortirale pri čemu je 46 krava (33,5%) bilo pozitivno na neki od ispitivanih agenasa. Prisustvo jednog od patogena je detektovano kod 43 krave (31,4%o), dok su uzorci od 3 krave (2,2%o) bili istovremeno pozitivni na 2 uzročnika. Prema ovim rezultatima, najzastupljeniji uzročnici su Neospora caninum i BVD virus koji su dokazani kod 23 (16,8%), odnosno 19 (13,8%) krava. Coxiella burnetii je dokazana kod 6 krava (4,4%), IBR virus kod jedne, dok prisustvo genoma Šmalenberg virusa nije ustanovljeno ni kod jedne krave. Trenutni rezultati ukazuju da su kontagiozni uzročnici abortusa prisutni u značajnoj meri na gazdinstvima PKB korporacije. Za njihovu kontrolu i otkrivanje neophodna je bliska saradnja veterinarske službe i dijagnostičke laboratorije.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014
T1  - Pobačaji goveda uzrokovani kontagioznim infektivnim agensima na gazdinstvima PKB korporacije tokom 2014. godine
VL  - 21
IS  - 3-4
SP  - 1
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1298
ER  - 

@article{
author = "Cvetojević, Đorđe and Savić, Božidar and Stanojević, Slobodan and Kureljušić, Branislav and Jezdimirović, Nemanja and Bojković-Kovačević, Slavica and Pavlović, Miloš and Katić, Marko and Jakić-Dimić, Dobrila",
year = "2015",
abstract = "Abortions represents significant problem in modern cattle husbandry because they directly and indirectly affects profit from production. Abortions in cattle can be caused by infectious (viruses, bacteria, protozoa, fungi) or non-infectious agents. Infectious agents can have beside their abortive effect other organ pathology which additionally compromise health of animal and also production. Also, some causative agents are zoonotic. This paper represents results from diagnostic examination of samples from cows which aborted on PKB farms during 2014. By polymerase chain reaction (PCR), we investigated presence of genom of BVD virus, IBR virus, Schmallenberg virus, Neospora caninum and Coxiella burnetii. Samples (fetus, placenta, swabs) from 137 cows which aborted were examined and 46 cows (33,5%) were positive on some of causative agents. Presence of one of pathogens was detected in 43 cows (31,4%o), while samples from 3 cows (2,2%o) were positive for two pathogens simultaneously. According to this results, the most common agents were Neospora caninum and BVD virus which were detected in 23 (16,8%) and 19 (13,8%) cows, respectively. Coxiella burnetii was proven in 6 cows (4,4%), IBR virus in one, while presence of Schmallenberg virus was not detected in any cow. Current results suggests that contagious abortive agents are present significantly on PKB corporation farms. Tight cooperation between veterinary service and diagnostic laboratory is necessary for their control and detection., Abortusi predstavljaju značajan problem u savremenom uzgoju goveda jer direktno i indirektno utiču na ekonomsku isplativost proizvodnje. Pobačaji goveda mogu biti izazvani infektivnim (virusi, bakterije, protozoe, gljivice) ili neinfektivnim faktorima. Infektivni agensi kao uzročnici pobačaja mogu pored abortogenog efekta imati i drugu organopatologiju čime dodatno ugrožavaju zdravstveno stanje životinja, a time i proizvodnju. Takođe, pojedini uzročnici imaju i zoonotski potencijal. U ovom radu prikazani su rezultati dijagnostičkih ispitivanja uzoraka poreklom od krava koje su abortirale sa gazdinstava PKB-a tokom 2014. godine. Metodom reakcije lančane polimeraze (PCR) ispitivano je prisustvo genoma BVD virusa, IBR virusa, Šmalenberg virusa, Neospora caninum i Coxiella burnetii. Pregledani su uzorci (fetus, placenta ili brisevi) od 137 krava koje su abortirale pri čemu je 46 krava (33,5%) bilo pozitivno na neki od ispitivanih agenasa. Prisustvo jednog od patogena je detektovano kod 43 krave (31,4%o), dok su uzorci od 3 krave (2,2%o) bili istovremeno pozitivni na 2 uzročnika. Prema ovim rezultatima, najzastupljeniji uzročnici su Neospora caninum i BVD virus koji su dokazani kod 23 (16,8%), odnosno 19 (13,8%) krava. Coxiella burnetii je dokazana kod 6 krava (4,4%), IBR virus kod jedne, dok prisustvo genoma Šmalenberg virusa nije ustanovljeno ni kod jedne krave. Trenutni rezultati ukazuju da su kontagiozni uzročnici abortusa prisutni u značajnoj meri na gazdinstvima PKB korporacije. Za njihovu kontrolu i otkrivanje neophodna je bliska saradnja veterinarske službe i dijagnostičke laboratorije.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014, Pobačaji goveda uzrokovani kontagioznim infektivnim agensima na gazdinstvima PKB korporacije tokom 2014. godine",
volume = "21",
number = "3-4",
pages = "1-6",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1298"
}

Cvetojević, Đ., Savić, B., Stanojević, S., Kureljušić, B., Jezdimirović, N., Bojković-Kovačević, S., Pavlović, M., Katić, M.,& Jakić-Dimić, D.. (2015). Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 21(3-4), 1-6.
https://hdl.handle.net/21.15107/rcub_veterinar_1298

Cvetojević Đ, Savić B, Stanojević S, Kureljušić B, Jezdimirović N, Bojković-Kovačević S, Pavlović M, Katić M, Jakić-Dimić D. Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2015;21(3-4):1-6.
https://hdl.handle.net/21.15107/rcub_veterinar_1298 .

Cvetojević, Đorđe, Savić, Božidar, Stanojević, Slobodan, Kureljušić, Branislav, Jezdimirović, Nemanja, Bojković-Kovačević, Slavica, Pavlović, Miloš, Katić, Marko, Jakić-Dimić, Dobrila, "Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014" in Zbornik naučnih radova Instituta PKB Agroekonomik, 21, no. 3-4 (2015):1-6,
https://hdl.handle.net/21.15107/rcub_veterinar_1298 .

TY  - CONF
AU  - Marković, Radmila
AU  - Šefer, Dragan
AU  - Jakić-Dimić, Dobrila
AU  - Jovanović, Dragoljub
AU  - Milić, Dragan
AU  - Radulović, Stamen
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2677
C3  - 19th Europian Symposium on Poultry Nutrition, Potsdam, Germany, August 26-29,2013
T1  - The effects of Detoxin-V on weight gain of broilers fed contaminated feed
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2677
ER  - 

@conference{
author = "Marković, Radmila and Šefer, Dragan and Jakić-Dimić, Dobrila and Jovanović, Dragoljub and Milić, Dragan and Radulović, Stamen",
year = "2013",
journal = "19th Europian Symposium on Poultry Nutrition, Potsdam, Germany, August 26-29,2013",
title = "The effects of Detoxin-V on weight gain of broilers fed contaminated feed",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2677"
}

Marković, R., Šefer, D., Jakić-Dimić, D., Jovanović, D., Milić, D.,& Radulović, S.. (2013). The effects of Detoxin-V on weight gain of broilers fed contaminated feed. in 19th Europian Symposium on Poultry Nutrition, Potsdam, Germany, August 26-29,2013.
https://hdl.handle.net/21.15107/rcub_veterinar_2677

Marković R, Šefer D, Jakić-Dimić D, Jovanović D, Milić D, Radulović S. The effects of Detoxin-V on weight gain of broilers fed contaminated feed. in 19th Europian Symposium on Poultry Nutrition, Potsdam, Germany, August 26-29,2013. 2013;.
https://hdl.handle.net/21.15107/rcub_veterinar_2677 .

Marković, Radmila, Šefer, Dragan, Jakić-Dimić, Dobrila, Jovanović, Dragoljub, Milić, Dragan, Radulović, Stamen, "The effects of Detoxin-V on weight gain of broilers fed contaminated feed" in 19th Europian Symposium on Poultry Nutrition, Potsdam, Germany, August 26-29,2013 (2013),
https://hdl.handle.net/21.15107/rcub_veterinar_2677 .

TY  - JOUR
AU  - Radulović, Stamen
AU  - Marković, Radmila
AU  - Milić, Dragan D.
AU  - Jakić-Dimić, Dobrila
AU  - Šefer, Dragan
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/982
AB  - The most common producers of mycotoxins are fungi of the genera Aspergillus, Fusarium and Penicillium. Toxins are of extreme importance because it can be transmitted from animals to humans through milk and animal products, some of which are carcinogenic and teratogenic. Mycotoxins cause a health disturbance of all animals, but the effects are more noticeable in highly productive animals in the farm way of keeping considering the much greater consumption of concentrate feeds, although forages also can be contaminated with mycotoxins in a significant manner. Mycotoxicoses are the most com­mon seasonal illnesses, and are an important diagnostic problem in veterinary practice, because its characteristics often resemble diseases caused by pathogens or nutritional deficiency or imbalance. The degree of health disturbances depends on the amount of toxins in feed and the length of intaking as on types and categories of animals. The presence of mycotoxins in animal feed is inevitable and therefore testing of raw materials and products is necessary so that feed for humans and animals can be safe for use. Damages arising as consequences of mycotoxicosis in poultry and swine production, due to the direct loss because of animals lossor, more commonly, indirectly due to the fall productive and reproductive performances of animals, imposed the need for continuous monitoring of the hygienic quality of feed mixtures for feeding these animals. During a five year period (2007-2012) were analyzed a total of 104 samples from the territory of Republic of Serbia intended for nutrition of all categories of poultry and mixtures for the initial and final fattening broilers (50 samples) and laying hens (54). The analysis included 57 samples of feed mixtures intended for all categories of swine - feed mixture for young (20 samples) and the old categories (37 samples) and 196 of the samples, which are commonly used in formulating rations for listed species (maize, soybean and sunflower meal). For the analysis of the samples was used thin layer chromatography and Elisa test. The results were compared with current Regulations on the quality of the feed (Official Gazette of RS 41/09) in force since 1.05.2010. in the part where the maximum allowable quantities of hazardous substances (Article 99) gives the value of the maximum permitted levels of mycotoxins in animal feed. The number and types of mycotoxins vary depending on the feed, as well as on the year which can be directly related to climatic factors, and the average annual humidity. The fact that in the samples was not detected the presence of aflatoxin indicate that in our conditions toxin producing fungi do not find fertile ground for the production of toxins, as well as the absence of certain nutrients in the production of complete feed mixtures for pigs and poultry, which are the traditional sources of aflatoxin (peanut oil meals). The results are encouraging given the fact a relatively small number of defective mixtures and nutrients. However, the fact that only a limited number of feed samples we received for the analysis suggests further caution and constant monitoring of the presence of mycotoxins in animal feed.
AB  - Mikotoksine najčešće proizvode gljivice iz rodova Aspergillus, Fusarium i Penicillium. Toksini su od izrazite važnosti jer se mogu preneti sa životinja na ljude putem mleka i životinjskih proizvoda, a neki od njih su kancerogeni i teratogeni. Mikotoksini dovode do poremećaja zdravstvenog stanja svih životinja, ali su efekti uočljiviji kod visoko proizvodnih životinja u farmskom načinu držanja s obzirom na znatno veću konzumaciju koncentrovanih hraniva iako i kabasta hraniva mogu da budu kontaminirana mikotoksinima u značajnijem stepenu. Mikotoksikoze su najčešće oboljenja sezonskog karaktera, a predstavljaju značajan dijagnostički problem za veterinarsku praksu, jer po karakteristikama često liče na oboljenja izazvana patogenim mikroorganizmima ili nutritivnim deficitom ili disbalansom. Stepen zdravstvenih poremećaja zavisi od količine toksina u hrani i dužine njegovog unošenja u organizam kao i od vrste i kategorije životinja Prisutnost mikotoksina u hrani za životinje je neizbežna pa je neophodno testiranje sirovina i proizvoda da bi hrana za ljude i životinje bila sigurna za upotrebu. Štete u živinarstvu i svinjarstvu koje nastaju usled mikotoksikoza, usled direktnih gubitaka zbog uginjavanja životinja ili, još češće, indirektne zbog pada proizvodnih i reproduktivnih sposobnosti životinja, nametnule su potrebu za kontinuiranim monitoringom higijenske ispravnosti krmnih smeša za ishranu ovih životinjskih vrsta. Tokom petogodišnjeg perioda (2007-2012) analizirana su ukupno 104 uzorka sa teritorije Republike Srbije namenjena ishrani svih kategorija živine i to smeše za početni i završni tov brojlera (50 uzoraka) i za kokoši nosilje (54). Analizom je obuhvaćeno i 57 uzoraka krmnih smeša namenjenih ishrani svih kategorija svinja i to smeše za ishranu mladih (20 uzoraka) i starih kategorija (37 uzoraka) kao i 196 uzoraka hraniva koja se najčešće koriste prilikom formulisanja obroka za navedene životinjske vrste (kukuruz, sojina i suncokretova sačma). Za analizu uzoraka korišćeni su metoda tankoslojne hromatografije i elisa test. Dobijeni rezultati su poređeni sa trenutno važećim Pravilnikom o kvalitetu hrane za životinje (Službeni Glasnik RS 41/09) koji se primenjuje od 1.05.2010. godine i gde se u delu o maksimalno dozvoljenim količinama štetnih materija (član 99) iznose vrednosti o maksimalno dozvoljenoj količini mikotoksina u hrani za životinje. Broj i vrsta mikotoksina varira u odnosu na vrstu smeša, kao i u odnosu na pojedine godine što se može dovesti u direktnu vezu sa klimatskim faktorima, odnosno prosečnom godišnjom vlažnošću. Činjenica da u ispitivanim uzorcima nije utvrđeno prisustvo aflatoksina ukazuje da u našim uslovima toksinprodukujuće gljivice ne nailaze na pogodno tle za produkciju ovog toksina, kao i na odsustvo pojedinih hraniva u proizvodnji potpunih krmnih smeša za svinje i živinu koje predstavljaju tradicionalne izvore aflatoksina (kikirikijeva uljana sačma). Dobijeni rezultati predstavljaju ohrabrujuću činjenicu s obzirom na relativno mali broj neispravnih smeša i hraniva. Međutim, činjenica da se radi o ograničenom broju uzoraka hrane koje smo dobijali na analizu upućuje na oprez i dalji konstantan monitoring prisustva mikotoksina u hrani za životinje.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Degree of mycotoxicological contamination of feed and complete feed mixtures for pigs and poultry during the period 2007-2012. on the territory of the Republic of Serbia
T1  - Stepen mikotoksikološke kontaminacije hraniva i potpunih krmnih smeša za ishranu svinja i živine tokom perioda 2007-2012. godine na teritoriji Republike Srbije
IS  - 124
SP  - 153
EP  - 169
DO  - 10.2298/ZMSPN1324153R
ER  - 

@article{
author = "Radulović, Stamen and Marković, Radmila and Milić, Dragan D. and Jakić-Dimić, Dobrila and Šefer, Dragan",
year = "2013",
abstract = "The most common producers of mycotoxins are fungi of the genera Aspergillus, Fusarium and Penicillium. Toxins are of extreme importance because it can be transmitted from animals to humans through milk and animal products, some of which are carcinogenic and teratogenic. Mycotoxins cause a health disturbance of all animals, but the effects are more noticeable in highly productive animals in the farm way of keeping considering the much greater consumption of concentrate feeds, although forages also can be contaminated with mycotoxins in a significant manner. Mycotoxicoses are the most com­mon seasonal illnesses, and are an important diagnostic problem in veterinary practice, because its characteristics often resemble diseases caused by pathogens or nutritional deficiency or imbalance. The degree of health disturbances depends on the amount of toxins in feed and the length of intaking as on types and categories of animals. The presence of mycotoxins in animal feed is inevitable and therefore testing of raw materials and products is necessary so that feed for humans and animals can be safe for use. Damages arising as consequences of mycotoxicosis in poultry and swine production, due to the direct loss because of animals lossor, more commonly, indirectly due to the fall productive and reproductive performances of animals, imposed the need for continuous monitoring of the hygienic quality of feed mixtures for feeding these animals. During a five year period (2007-2012) were analyzed a total of 104 samples from the territory of Republic of Serbia intended for nutrition of all categories of poultry and mixtures for the initial and final fattening broilers (50 samples) and laying hens (54). The analysis included 57 samples of feed mixtures intended for all categories of swine - feed mixture for young (20 samples) and the old categories (37 samples) and 196 of the samples, which are commonly used in formulating rations for listed species (maize, soybean and sunflower meal). For the analysis of the samples was used thin layer chromatography and Elisa test. The results were compared with current Regulations on the quality of the feed (Official Gazette of RS 41/09) in force since 1.05.2010. in the part where the maximum allowable quantities of hazardous substances (Article 99) gives the value of the maximum permitted levels of mycotoxins in animal feed. The number and types of mycotoxins vary depending on the feed, as well as on the year which can be directly related to climatic factors, and the average annual humidity. The fact that in the samples was not detected the presence of aflatoxin indicate that in our conditions toxin producing fungi do not find fertile ground for the production of toxins, as well as the absence of certain nutrients in the production of complete feed mixtures for pigs and poultry, which are the traditional sources of aflatoxin (peanut oil meals). The results are encouraging given the fact a relatively small number of defective mixtures and nutrients. However, the fact that only a limited number of feed samples we received for the analysis suggests further caution and constant monitoring of the presence of mycotoxins in animal feed., Mikotoksine najčešće proizvode gljivice iz rodova Aspergillus, Fusarium i Penicillium. Toksini su od izrazite važnosti jer se mogu preneti sa životinja na ljude putem mleka i životinjskih proizvoda, a neki od njih su kancerogeni i teratogeni. Mikotoksini dovode do poremećaja zdravstvenog stanja svih životinja, ali su efekti uočljiviji kod visoko proizvodnih životinja u farmskom načinu držanja s obzirom na znatno veću konzumaciju koncentrovanih hraniva iako i kabasta hraniva mogu da budu kontaminirana mikotoksinima u značajnijem stepenu. Mikotoksikoze su najčešće oboljenja sezonskog karaktera, a predstavljaju značajan dijagnostički problem za veterinarsku praksu, jer po karakteristikama često liče na oboljenja izazvana patogenim mikroorganizmima ili nutritivnim deficitom ili disbalansom. Stepen zdravstvenih poremećaja zavisi od količine toksina u hrani i dužine njegovog unošenja u organizam kao i od vrste i kategorije životinja Prisutnost mikotoksina u hrani za životinje je neizbežna pa je neophodno testiranje sirovina i proizvoda da bi hrana za ljude i životinje bila sigurna za upotrebu. Štete u živinarstvu i svinjarstvu koje nastaju usled mikotoksikoza, usled direktnih gubitaka zbog uginjavanja životinja ili, još češće, indirektne zbog pada proizvodnih i reproduktivnih sposobnosti životinja, nametnule su potrebu za kontinuiranim monitoringom higijenske ispravnosti krmnih smeša za ishranu ovih životinjskih vrsta. Tokom petogodišnjeg perioda (2007-2012) analizirana su ukupno 104 uzorka sa teritorije Republike Srbije namenjena ishrani svih kategorija živine i to smeše za početni i završni tov brojlera (50 uzoraka) i za kokoši nosilje (54). Analizom je obuhvaćeno i 57 uzoraka krmnih smeša namenjenih ishrani svih kategorija svinja i to smeše za ishranu mladih (20 uzoraka) i starih kategorija (37 uzoraka) kao i 196 uzoraka hraniva koja se najčešće koriste prilikom formulisanja obroka za navedene životinjske vrste (kukuruz, sojina i suncokretova sačma). Za analizu uzoraka korišćeni su metoda tankoslojne hromatografije i elisa test. Dobijeni rezultati su poređeni sa trenutno važećim Pravilnikom o kvalitetu hrane za životinje (Službeni Glasnik RS 41/09) koji se primenjuje od 1.05.2010. godine i gde se u delu o maksimalno dozvoljenim količinama štetnih materija (član 99) iznose vrednosti o maksimalno dozvoljenoj količini mikotoksina u hrani za životinje. Broj i vrsta mikotoksina varira u odnosu na vrstu smeša, kao i u odnosu na pojedine godine što se može dovesti u direktnu vezu sa klimatskim faktorima, odnosno prosečnom godišnjom vlažnošću. Činjenica da u ispitivanim uzorcima nije utvrđeno prisustvo aflatoksina ukazuje da u našim uslovima toksinprodukujuće gljivice ne nailaze na pogodno tle za produkciju ovog toksina, kao i na odsustvo pojedinih hraniva u proizvodnji potpunih krmnih smeša za svinje i živinu koje predstavljaju tradicionalne izvore aflatoksina (kikirikijeva uljana sačma). Dobijeni rezultati predstavljaju ohrabrujuću činjenicu s obzirom na relativno mali broj neispravnih smeša i hraniva. Međutim, činjenica da se radi o ograničenom broju uzoraka hrane koje smo dobijali na analizu upućuje na oprez i dalji konstantan monitoring prisustva mikotoksina u hrani za životinje.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Degree of mycotoxicological contamination of feed and complete feed mixtures for pigs and poultry during the period 2007-2012. on the territory of the Republic of Serbia, Stepen mikotoksikološke kontaminacije hraniva i potpunih krmnih smeša za ishranu svinja i živine tokom perioda 2007-2012. godine na teritoriji Republike Srbije",
number = "124",
pages = "153-169",
doi = "10.2298/ZMSPN1324153R"
}

Radulović, S., Marković, R., Milić, D. D., Jakić-Dimić, D.,& Šefer, D.. (2013). Degree of mycotoxicological contamination of feed and complete feed mixtures for pigs and poultry during the period 2007-2012. on the territory of the Republic of Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 153-169.
https://doi.org/10.2298/ZMSPN1324153R

Radulović S, Marković R, Milić DD, Jakić-Dimić D, Šefer D. Degree of mycotoxicological contamination of feed and complete feed mixtures for pigs and poultry during the period 2007-2012. on the territory of the Republic of Serbia. in Zbornik Matice srpske za prirodne nauke. 2013;(124):153-169.
doi:10.2298/ZMSPN1324153R .

Radulović, Stamen, Marković, Radmila, Milić, Dragan D., Jakić-Dimić, Dobrila, Šefer, Dragan, "Degree of mycotoxicological contamination of feed and complete feed mixtures for pigs and poultry during the period 2007-2012. on the territory of the Republic of Serbia" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):153-169,
https://doi.org/10.2298/ZMSPN1324153R . .

TY  - CONF
AU  - Radulović, Stamen
AU  - Šefer, Dragan
AU  - Jakić-Dimić, Dobrila
AU  - Marković, Radmila
AU  - Baltić, Milan Ž.
AU  - Petrujkić, Branko
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/928
AB  - Creatine monohydrate (CMH) has become a popular dietary supplement used by athletes to increase muscle performance. Creatine acts in muscles in the form of creatine phosphate, which provides muscle with ATP. The increased concentration of intramuscular kreatinfosfat draws water into muscle cells, thus increasing cell volume. It has been reported a significant increase in total body mass and lean body mass in CMH intake in humans, suggesting that pigs with the addition of CMH could showe a linear increase in growth during the period of complementary feeding. Creatine is not present in the feed of plant origin that requires the use of feed of animal origin as its source in diets for animals or the use of synthetic products. Among the feed of animal origin that is commonly used in formulating meal for pigs, the greatest amount of creatine is found in meat and bone meal (207.7 mg/kg), fish meal (1110.5 mg/kg) and poultry by product meal (201 mg/kg), with variations depending on the quality of raw materials and production process for obtaining. Currently there are three different synthetic forms of creatine at the market: monohydrate, phosphate and citrate. The amount and length of adding CMH may be the reason for the variation in the quality of meat in the different groups of pigs. It was found that water loss, and firmness of M. longissimus are improved due to CMHC addition into the meal for fattening pigs in the amount of 3 g daily for 30 days, or 25 grams daily for five days before slaughter. Adding creatine in small amounts over a longer period of time can be just effective as addition of large quantities of creatine in a shorter period of time.
AB  - Kreatin je amino kiselinski derivat koji se normalno proizvodi u jetri, bubrezima i pankreasu od glicina, arginina i metionina. Kreatin monohidrat (CMH) je postao popularan dodatak u ishrani sportista koji ga koriste radi povećanja mišićnih performansi (Greenhaff i sar., 1993), kao i za smanjenje zamora usled vežbanja visokog intenziteta. Navedeno povećanje performansi uključuje i povećanje mišićne mase (Balsom i sar, 1995.), tako da je dokazano da sportisti konzumiranjem 20 g CMH dnevno tokom šest dana povećavaju telesnu masu za 1,1 kg. Dodavanje kreatina utiče na porast ćelijske hidratacije (Juhn, 1999), što je anabolički proliferativni signal za sintezu proteina (Haussinger i sar., 1996). Istraživanja na ljudima (Greenhaff, 1996) su pokazala da dodavanje kreatin monohidrata u količini od 20 g dnevno tokom pet dana povećava količinu intramuskularnog kreatina 20%. Harris i sar. (1992) su prvi izvestili da je oralno unošenje CMH rezultovalo povećanjem koncentracije intramuskularnog kreatina i kreatin fosfata. Autori su otkrili da je svakodnevno konzumiranje 5 g CMH četiri do šest puta na dan tokom dva ili više dana dovelo do značajnog povećanja ukupne koncentracije kreatina u skeletnim mišićima ljudi. Istraživanja su pokazala dva moguća mehanizma kojim kreatin povećava rast mišića i sintezu proteina. Mišić punjen kreatinom ima kapacitet za povećanje rada ili snage usled povećanja količine energije skladištene kao fosfokreatin (odgovoran za refosforilaciju adenozin-difosfata) i odlaganje početka mišićnog zamaranja (Casey i sar., 1996). Povećana koncentracija kreatin-fosfata u mišićima privlači vodu u mišićne ćelije i na taj način povećava zapreminu ćelije (Hultman i sar., 1996). Prevost i sar. (1997) su izvestili da intramuskularno punjenje kreatinom može da puferuje akumuliranje mlečne kiseline i ubrza vreme oporavka od kratkotrajnog vežbanja maksimalinog intenziteta. Kreatin nije prisutan u hranivima biljnog porekla što nalaže upotrebu hraniva animalnog porekla kao njegovog izvora u obrocima za životinje ili upotrebu sintetskih preparata. Među hranivima animalnog porekla koja se uobičajeno koriste prilikom formulisanja obroka za svinje najveća količina kreatina se nalazi u mesno-koštanom brašnu (207,7 mg/kg), ribljem brašnu (1110,5 mg/kg) i brašnu od otpadaka pri klanju živine (201 mg/kg) uz variranja u zavisnosti od kvaliteta sirovine i tehnološkog postupka dobijanja. Na tržištu su trenutno prisutne tri različite forme kreatina: monohidrat, fosfat i citrat. Kreatin monohidrat sadrži 88% kreatina i 12% vode, odnosno 1 g kreatin monohidrata sadrži 880 mg kreatina. Kreatin fosfat sadrži 62,3% kreatina i 37,7% fosfata, i trenutno je dosta skuplji od kreatin monohidrata. Kreatin citrat sadrži tek 400 mg kreatina po gramu preparata i bolje je rastvorljiv u vodi. Do sada je najviše istraživanja obavljeno sa kreatin monohidratom koji je u odnosu na ostale forme kreatina najviše u upotrebi kao dodatak u ishrani sportista. Iako je upotreba kreatina najviše proučavana kod ljudi, postoji mogućnost njegove upotrebe i u ishrani svinja. Opisanim mehanizmima povećanja ćelijske hidratacije i sinteze proteina mogli bi se poboljšati prirast i/ili mesnatost trupa svinja hranjenih sa dodatkom kreatin monohidrata (CMH).
PB  - Institut PKB Agroekonomik, Padinska skela
C3  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - The use of creatine in the pigs nutrition
T1  - Upotreba kreatina u ishrani svinja
VL  - 18
IS  - 3-4
SP  - 101
EP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_928
ER  - 

@conference{
author = "Radulović, Stamen and Šefer, Dragan and Jakić-Dimić, Dobrila and Marković, Radmila and Baltić, Milan Ž. and Petrujkić, Branko",
year = "2012",
abstract = "Creatine monohydrate (CMH) has become a popular dietary supplement used by athletes to increase muscle performance. Creatine acts in muscles in the form of creatine phosphate, which provides muscle with ATP. The increased concentration of intramuscular kreatinfosfat draws water into muscle cells, thus increasing cell volume. It has been reported a significant increase in total body mass and lean body mass in CMH intake in humans, suggesting that pigs with the addition of CMH could showe a linear increase in growth during the period of complementary feeding. Creatine is not present in the feed of plant origin that requires the use of feed of animal origin as its source in diets for animals or the use of synthetic products. Among the feed of animal origin that is commonly used in formulating meal for pigs, the greatest amount of creatine is found in meat and bone meal (207.7 mg/kg), fish meal (1110.5 mg/kg) and poultry by product meal (201 mg/kg), with variations depending on the quality of raw materials and production process for obtaining. Currently there are three different synthetic forms of creatine at the market: monohydrate, phosphate and citrate. The amount and length of adding CMH may be the reason for the variation in the quality of meat in the different groups of pigs. It was found that water loss, and firmness of M. longissimus are improved due to CMHC addition into the meal for fattening pigs in the amount of 3 g daily for 30 days, or 25 grams daily for five days before slaughter. Adding creatine in small amounts over a longer period of time can be just effective as addition of large quantities of creatine in a shorter period of time., Kreatin je amino kiselinski derivat koji se normalno proizvodi u jetri, bubrezima i pankreasu od glicina, arginina i metionina. Kreatin monohidrat (CMH) je postao popularan dodatak u ishrani sportista koji ga koriste radi povećanja mišićnih performansi (Greenhaff i sar., 1993), kao i za smanjenje zamora usled vežbanja visokog intenziteta. Navedeno povećanje performansi uključuje i povećanje mišićne mase (Balsom i sar, 1995.), tako da je dokazano da sportisti konzumiranjem 20 g CMH dnevno tokom šest dana povećavaju telesnu masu za 1,1 kg. Dodavanje kreatina utiče na porast ćelijske hidratacije (Juhn, 1999), što je anabolički proliferativni signal za sintezu proteina (Haussinger i sar., 1996). Istraživanja na ljudima (Greenhaff, 1996) su pokazala da dodavanje kreatin monohidrata u količini od 20 g dnevno tokom pet dana povećava količinu intramuskularnog kreatina 20%. Harris i sar. (1992) su prvi izvestili da je oralno unošenje CMH rezultovalo povećanjem koncentracije intramuskularnog kreatina i kreatin fosfata. Autori su otkrili da je svakodnevno konzumiranje 5 g CMH četiri do šest puta na dan tokom dva ili više dana dovelo do značajnog povećanja ukupne koncentracije kreatina u skeletnim mišićima ljudi. Istraživanja su pokazala dva moguća mehanizma kojim kreatin povećava rast mišića i sintezu proteina. Mišić punjen kreatinom ima kapacitet za povećanje rada ili snage usled povećanja količine energije skladištene kao fosfokreatin (odgovoran za refosforilaciju adenozin-difosfata) i odlaganje početka mišićnog zamaranja (Casey i sar., 1996). Povećana koncentracija kreatin-fosfata u mišićima privlači vodu u mišićne ćelije i na taj način povećava zapreminu ćelije (Hultman i sar., 1996). Prevost i sar. (1997) su izvestili da intramuskularno punjenje kreatinom može da puferuje akumuliranje mlečne kiseline i ubrza vreme oporavka od kratkotrajnog vežbanja maksimalinog intenziteta. Kreatin nije prisutan u hranivima biljnog porekla što nalaže upotrebu hraniva animalnog porekla kao njegovog izvora u obrocima za životinje ili upotrebu sintetskih preparata. Među hranivima animalnog porekla koja se uobičajeno koriste prilikom formulisanja obroka za svinje najveća količina kreatina se nalazi u mesno-koštanom brašnu (207,7 mg/kg), ribljem brašnu (1110,5 mg/kg) i brašnu od otpadaka pri klanju živine (201 mg/kg) uz variranja u zavisnosti od kvaliteta sirovine i tehnološkog postupka dobijanja. Na tržištu su trenutno prisutne tri različite forme kreatina: monohidrat, fosfat i citrat. Kreatin monohidrat sadrži 88% kreatina i 12% vode, odnosno 1 g kreatin monohidrata sadrži 880 mg kreatina. Kreatin fosfat sadrži 62,3% kreatina i 37,7% fosfata, i trenutno je dosta skuplji od kreatin monohidrata. Kreatin citrat sadrži tek 400 mg kreatina po gramu preparata i bolje je rastvorljiv u vodi. Do sada je najviše istraživanja obavljeno sa kreatin monohidratom koji je u odnosu na ostale forme kreatina najviše u upotrebi kao dodatak u ishrani sportista. Iako je upotreba kreatina najviše proučavana kod ljudi, postoji mogućnost njegove upotrebe i u ishrani svinja. Opisanim mehanizmima povećanja ćelijske hidratacije i sinteze proteina mogli bi se poboljšati prirast i/ili mesnatost trupa svinja hranjenih sa dodatkom kreatin monohidrata (CMH).",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "The use of creatine in the pigs nutrition, Upotreba kreatina u ishrani svinja",
volume = "18",
number = "3-4",
pages = "101-109",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_928"
}

Radulović, S., Šefer, D., Jakić-Dimić, D., Marković, R., Baltić, M. Ž.,& Petrujkić, B.. (2012). The use of creatine in the pigs nutrition. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 18(3-4), 101-109.
https://hdl.handle.net/21.15107/rcub_veterinar_928

Radulović S, Šefer D, Jakić-Dimić D, Marković R, Baltić MŽ, Petrujkić B. The use of creatine in the pigs nutrition. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2012;18(3-4):101-109.
https://hdl.handle.net/21.15107/rcub_veterinar_928 .

Radulović, Stamen, Šefer, Dragan, Jakić-Dimić, Dobrila, Marković, Radmila, Baltić, Milan Ž., Petrujkić, Branko, "The use of creatine in the pigs nutrition" in Zbornik naučnih radova Instituta PKB Agroekonomik, 18, no. 3-4 (2012):101-109,
https://hdl.handle.net/21.15107/rcub_veterinar_928 .

TY  - CONF
AU  - Marković, Radmila
AU  - Todorović, M.D.
AU  - Baltić, Milan Ž.
AU  - Radulović, Stamen
AU  - Dokmanović, Marija
AU  - Drljačić, Aleksandar
AU  - Šefer, Dragan
AU  - Jakić-Dimić, Dobrila
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/869
AB  - The aim of this study was to investigate the possibility of using different feed compound for pigs in order to increase content of n-3 fatty acids in adipose tissue, as well as improving relation n-6/n-3 fatty acid that is known to be disordered in the modern way peoples diet. In this trial to view this crossbred pigs were used (Yorkshire × Landrace), with an initial body weight of 60 kg. The pigs were divided into two groups of 10 pigs and fed standard diets for fattening pigs of 60-100 kg (finisher), provided differences between groups only in the S experimental group which had a full-fat soybean in feed mixtures (S), and a second group enriched linseed (L) in the recommended amount of 2.5% in the feed mixture (Vitalan, Vitalac, France). Vitalan contained 85% of extruded linseed and the rest were wheat bran and antioxidants. The mixtures were balanced and fully meet the requirements of the animals at this stage of fattening. Samples were collected for testing of complete mixtures of chemical composition and fatty acid content. Pigs were kept until weight of 105 kg. At the end of the experiment, after slaughtering, processing and cooling carcass samples of adipose tissue (back) were taken of each pig in both groups. The adipose tissue was examined of fatty acid composition. Chemical analysis of fatty acid composition (a gas chromatograph GC/MSD GC 6890, MS 5972/73) complete feed mixtures showed that the feed with linseed (L) had significantly lower (P  lt 0.001) content of SFA, and significantly higher content (p lt 0.001) PUFA from feed mixtures with full-fat soybean (S). Also the ratio of n-6/n-3 in feed mixture in L group (5.567) was lower compared to feed mixture in S group (11.00) which is statistically highly significant (P lt 0.001). By analyzing the fatty acid composition of pigs fat statistically significant differences were found between experimental groups. In adipose tissue of pigs in the groups with linseed (L) in mixtures was achieved significantly (p lt 0.001) higher content of n-3 fatty acids, or 1.97% compared to 1.42% in groups receiving full-fat soybean (S) in feed mixtures, and significantly (p lt 0.001) lower content of n-6 fatty acids, or 20.09% compared to 24.91%. Ratio n-6/n-3 in adipose tissue for the L group was significantly lower (p lt 0.001) or 10.23 relative to the group S (17.74).
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - The influence of feeds on fatty acid composition of meat fattening pigs
SP  - 1576
EP  - 1581
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_869
ER  - 

@conference{
author = "Marković, Radmila and Todorović, M.D. and Baltić, Milan Ž. and Radulović, Stamen and Dokmanović, Marija and Drljačić, Aleksandar and Šefer, Dragan and Jakić-Dimić, Dobrila",
year = "2012",
abstract = "The aim of this study was to investigate the possibility of using different feed compound for pigs in order to increase content of n-3 fatty acids in adipose tissue, as well as improving relation n-6/n-3 fatty acid that is known to be disordered in the modern way peoples diet. In this trial to view this crossbred pigs were used (Yorkshire × Landrace), with an initial body weight of 60 kg. The pigs were divided into two groups of 10 pigs and fed standard diets for fattening pigs of 60-100 kg (finisher), provided differences between groups only in the S experimental group which had a full-fat soybean in feed mixtures (S), and a second group enriched linseed (L) in the recommended amount of 2.5% in the feed mixture (Vitalan, Vitalac, France). Vitalan contained 85% of extruded linseed and the rest were wheat bran and antioxidants. The mixtures were balanced and fully meet the requirements of the animals at this stage of fattening. Samples were collected for testing of complete mixtures of chemical composition and fatty acid content. Pigs were kept until weight of 105 kg. At the end of the experiment, after slaughtering, processing and cooling carcass samples of adipose tissue (back) were taken of each pig in both groups. The adipose tissue was examined of fatty acid composition. Chemical analysis of fatty acid composition (a gas chromatograph GC/MSD GC 6890, MS 5972/73) complete feed mixtures showed that the feed with linseed (L) had significantly lower (P  lt 0.001) content of SFA, and significantly higher content (p lt 0.001) PUFA from feed mixtures with full-fat soybean (S). Also the ratio of n-6/n-3 in feed mixture in L group (5.567) was lower compared to feed mixture in S group (11.00) which is statistically highly significant (P lt 0.001). By analyzing the fatty acid composition of pigs fat statistically significant differences were found between experimental groups. In adipose tissue of pigs in the groups with linseed (L) in mixtures was achieved significantly (p lt 0.001) higher content of n-3 fatty acids, or 1.97% compared to 1.42% in groups receiving full-fat soybean (S) in feed mixtures, and significantly (p lt 0.001) lower content of n-6 fatty acids, or 20.09% compared to 24.91%. Ratio n-6/n-3 in adipose tissue for the L group was significantly lower (p lt 0.001) or 10.23 relative to the group S (17.74).",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "The influence of feeds on fatty acid composition of meat fattening pigs",
pages = "1576-1581",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_869"
}

Marković, R., Todorović, M.D., Baltić, M. Ž., Radulović, S., Dokmanović, M., Drljačić, A., Šefer, D.,& Jakić-Dimić, D.. (2012). The influence of feeds on fatty acid composition of meat fattening pigs. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 1576-1581.
https://hdl.handle.net/21.15107/rcub_veterinar_869

Marković R, Todorović M, Baltić MŽ, Radulović S, Dokmanović M, Drljačić A, Šefer D, Jakić-Dimić D. The influence of feeds on fatty acid composition of meat fattening pigs. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:1576-1581.
https://hdl.handle.net/21.15107/rcub_veterinar_869 .

Marković, Radmila, Todorović, M.D., Baltić, Milan Ž., Radulović, Stamen, Dokmanović, Marija, Drljačić, Aleksandar, Šefer, Dragan, Jakić-Dimić, Dobrila, "The influence of feeds on fatty acid composition of meat fattening pigs" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):1576-1581,
https://hdl.handle.net/21.15107/rcub_veterinar_869 .

TY  - JOUR
AU  - Radulović, Stamen
AU  - Šefer, Dragan
AU  - Marković, Radmila
AU  - Jakić-Dimić, Dobrila
AU  - Grdović, Svetlana
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2087
AB  - Kreatin  je  ergogeno  pomoćno  sredstvo  koje  odnedavno  uživa  veliku  pažnju  domaće  i  strane  naučne 
javnosti kao potencijalni stimulator rasta u ishrani životinja. Utvrđeno je značajno povećanje ukupne telesne 
mase i čiste mišićne mase prilikom unosa kreatina kod ljudi, što je navelo na zaključak da bi se dodavanjem 
kreatina  u  hrani  za  živinu  uticalo  na  poboljšanje  proizvodnih  rezultata.  Utvrđena  je    bolja  konverzija  kod 
piladi hranjenih obrokom sa dodatkom 0,63% kreatina. Upotrebom obroka sa dodatkom 5% ili 10% kreatina 
smanjen  je  nivo masti  u  abdomenu  kod  brojlera.  Histopatološke  promene  nisu  bile  uočene  u  ogledima  na 
živini, što dokazuje da kreatin nema patološki uticaj na jetru, bubrege i mišiće.
AB  - Creatine  is  an  ergogenic  supplement  that  from  recently  enjoys  great  attention  of    national  and 
international scientific community as a potential growth promoter in animal nutrition. A significant increase 
in total body mass and lean body mass during creatine ingestion in humans was observed, which led to the 
conclusion  that  the  poultry  with  added  creatine  could  showe  an  increase  in  growth  during  the  period  of 
complementary  feeding. There  was  a  better  conversion  observed  in  chickens  fed  diets  supplemented  with 
0.63% of creatine. Using a meal with the addition of 5 or 10% creatine has reduced the level of abdominal fat 
in broilers. Histopathological changes were not significant in studies on poultry proving that creatine has no 
pathological effect on the liver, kidneys and muscles.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Efekti upotrebe kreatina kao stimulatora rasta u ishrani živine
T1  - The effects of using creatine as a growth promoter in poultry nutrition
VL  - 12
IS  - 1
SP  - 66
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2087
ER  - 

@article{
author = "Radulović, Stamen and Šefer, Dragan and Marković, Radmila and Jakić-Dimić, Dobrila and Grdović, Svetlana",
year = "2012",
abstract = "Kreatin  je  ergogeno  pomoćno  sredstvo  koje  odnedavno  uživa  veliku  pažnju  domaće  i  strane  naučne 
javnosti kao potencijalni stimulator rasta u ishrani životinja. Utvrđeno je značajno povećanje ukupne telesne 
mase i čiste mišićne mase prilikom unosa kreatina kod ljudi, što je navelo na zaključak da bi se dodavanjem 
kreatina  u  hrani  za  živinu  uticalo  na  poboljšanje  proizvodnih  rezultata.  Utvrđena  je    bolja  konverzija  kod 
piladi hranjenih obrokom sa dodatkom 0,63% kreatina. Upotrebom obroka sa dodatkom 5% ili 10% kreatina 
smanjen  je  nivo masti  u  abdomenu  kod  brojlera.  Histopatološke  promene  nisu  bile  uočene  u  ogledima  na 
živini, što dokazuje da kreatin nema patološki uticaj na jetru, bubrege i mišiće., Creatine  is  an  ergogenic  supplement  that  from  recently  enjoys  great  attention  of    national  and 
international scientific community as a potential growth promoter in animal nutrition. A significant increase 
in total body mass and lean body mass during creatine ingestion in humans was observed, which led to the 
conclusion  that  the  poultry  with  added  creatine  could  showe  an  increase  in  growth  during  the  period  of 
complementary  feeding. There  was  a  better  conversion  observed  in  chickens  fed  diets  supplemented  with 
0.63% of creatine. Using a meal with the addition of 5 or 10% creatine has reduced the level of abdominal fat 
in broilers. Histopathological changes were not significant in studies on poultry proving that creatine has no 
pathological effect on the liver, kidneys and muscles.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Efekti upotrebe kreatina kao stimulatora rasta u ishrani živine, The effects of using creatine as a growth promoter in poultry nutrition",
volume = "12",
number = "1",
pages = "66-73",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2087"
}

Radulović, S., Šefer, D., Marković, R., Jakić-Dimić, D.,& Grdović, S.. (2012). Efekti upotrebe kreatina kao stimulatora rasta u ishrani živine. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 12(1), 66-73.
https://hdl.handle.net/21.15107/rcub_veterinar_2087

Radulović S, Šefer D, Marković R, Jakić-Dimić D, Grdović S. Efekti upotrebe kreatina kao stimulatora rasta u ishrani živine. in Veterinarski žurnal Republike Srpske. 2012;12(1):66-73.
https://hdl.handle.net/21.15107/rcub_veterinar_2087 .

Radulović, Stamen, Šefer, Dragan, Marković, Radmila, Jakić-Dimić, Dobrila, Grdović, Svetlana, "Efekti upotrebe kreatina kao stimulatora rasta u ishrani živine" in Veterinarski žurnal Republike Srpske, 12, no. 1 (2012):66-73,
https://hdl.handle.net/21.15107/rcub_veterinar_2087 .

TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Jakić-Dimić, Dobrila
AU  - Bojkovski, Jovan
AU  - Prodanović, Radiša
AU  - Kureljušić, Branislav
AU  - Potkonjak, Aleksandar
AU  - Savić, Borivoje
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/889
AB  - Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.
PB  - Springer Wien, Wien
T2  - Archives of Virology
T1  - Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia
VL  - 157
IS  - 1
SP  - 21
EP  - 28
DO  - 10.1007/s00705-011-1130-9
ER  - 

@article{
author = "Savić, Božidar and Milićević, Vesna and Jakić-Dimić, Dobrila and Bojkovski, Jovan and Prodanović, Radiša and Kureljušić, Branislav and Potkonjak, Aleksandar and Savić, Borivoje",
year = "2012",
abstract = "Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.",
publisher = "Springer Wien, Wien",
journal = "Archives of Virology",
title = "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia",
volume = "157",
number = "1",
pages = "21-28",
doi = "10.1007/s00705-011-1130-9"
}

Savić, B., Milićević, V., Jakić-Dimić, D., Bojkovski, J., Prodanović, R., Kureljušić, B., Potkonjak, A.,& Savić, B.. (2012). Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology
Springer Wien, Wien., 157(1), 21-28.
https://doi.org/10.1007/s00705-011-1130-9

Savić B, Milićević V, Jakić-Dimić D, Bojkovski J, Prodanović R, Kureljušić B, Potkonjak A, Savić B. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology. 2012;157(1):21-28.
doi:10.1007/s00705-011-1130-9 .

Savić, Božidar, Milićević, Vesna, Jakić-Dimić, Dobrila, Bojkovski, Jovan, Prodanović, Radiša, Kureljušić, Branislav, Potkonjak, Aleksandar, Savić, Borivoje, "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia" in Archives of Virology, 157, no. 1 (2012):21-28,
https://doi.org/10.1007/s00705-011-1130-9 . .

TY  - CONF
AU  - Kureljušić, Jasna
AU  - Jakić-Dimić, Dobrila
AU  - Kureljušić, Branislav
AU  - Nešić, Ksenija
AU  - Jezdimirović, Nemanja
AU  - Šefer, Dragan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/895
AB  - Aspergillosis is frequent fungal disease of different avian and mammal species, caused by fungi of genus Aspergillus. The disease is characterized by inflammatory changes in the respiratory system and sometimes has generalized onset when more organ systems are affected. These fungi are well known aflatoxin producers which, besides other microorganisms, can also be found in feed raw materials. However, the presence of fungi is not a proof of mycotoxins contamination, because they are produced in specific conditions. In this paper are presented results of examination of a flock of turkey poults, 21 days old, at one farm in Serbia. Clinical signs of central nervous system disorder were observed. After necropsy, in ten out of twelve necropsied turkey poults multiple yellowish-white granulomas on lungs were found. In nine out of twelve necropsied turkey poults solitary yellowishwhite granuloma on sagital section of the cerebrum or cerebellum were found. Mycological finding revealed fungi Aspergillus fumigatus. Two stain methods were used: haematoxylin-eosin (HE) and Grocott methenamine silver (GMS). Histopathological analysys of lung and brain has revealed the presence of granulomatous foci and caseous necrosis with surrounding region of proliferation including giant cells, macrophages, heterophils and lymphocytes and outer capsule of connective tissue. The fungal hyphae were hardly visible or not in HE stained sections, while septed and arborized hyphae were easily demonstrated by GMS method predominantly in central parts of granuloma. Mycological examination of feed showed the presence of Aspergillus fumigatus and Aspergillus flavus, as well as ELISA detectable level of aflatoxins (3.00 μg/kg).
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Feed as cause of aspergillosis in flock of Turkey poults
SP  - 1547
EP  - 1552
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_895
ER  - 

@conference{
author = "Kureljušić, Jasna and Jakić-Dimić, Dobrila and Kureljušić, Branislav and Nešić, Ksenija and Jezdimirović, Nemanja and Šefer, Dragan",
year = "2012",
abstract = "Aspergillosis is frequent fungal disease of different avian and mammal species, caused by fungi of genus Aspergillus. The disease is characterized by inflammatory changes in the respiratory system and sometimes has generalized onset when more organ systems are affected. These fungi are well known aflatoxin producers which, besides other microorganisms, can also be found in feed raw materials. However, the presence of fungi is not a proof of mycotoxins contamination, because they are produced in specific conditions. In this paper are presented results of examination of a flock of turkey poults, 21 days old, at one farm in Serbia. Clinical signs of central nervous system disorder were observed. After necropsy, in ten out of twelve necropsied turkey poults multiple yellowish-white granulomas on lungs were found. In nine out of twelve necropsied turkey poults solitary yellowishwhite granuloma on sagital section of the cerebrum or cerebellum were found. Mycological finding revealed fungi Aspergillus fumigatus. Two stain methods were used: haematoxylin-eosin (HE) and Grocott methenamine silver (GMS). Histopathological analysys of lung and brain has revealed the presence of granulomatous foci and caseous necrosis with surrounding region of proliferation including giant cells, macrophages, heterophils and lymphocytes and outer capsule of connective tissue. The fungal hyphae were hardly visible or not in HE stained sections, while septed and arborized hyphae were easily demonstrated by GMS method predominantly in central parts of granuloma. Mycological examination of feed showed the presence of Aspergillus fumigatus and Aspergillus flavus, as well as ELISA detectable level of aflatoxins (3.00 μg/kg).",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Feed as cause of aspergillosis in flock of Turkey poults",
pages = "1547-1552",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_895"
}

Kureljušić, J., Jakić-Dimić, D., Kureljušić, B., Nešić, K., Jezdimirović, N.,& Šefer, D.. (2012). Feed as cause of aspergillosis in flock of Turkey poults. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 1547-1552.
https://hdl.handle.net/21.15107/rcub_veterinar_895

Kureljušić J, Jakić-Dimić D, Kureljušić B, Nešić K, Jezdimirović N, Šefer D. Feed as cause of aspergillosis in flock of Turkey poults. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:1547-1552.
https://hdl.handle.net/21.15107/rcub_veterinar_895 .

Kureljušić, Jasna, Jakić-Dimić, Dobrila, Kureljušić, Branislav, Nešić, Ksenija, Jezdimirović, Nemanja, Šefer, Dragan, "Feed as cause of aspergillosis in flock of Turkey poults" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):1547-1552,
https://hdl.handle.net/21.15107/rcub_veterinar_895 .

TY  - JOUR
AU  - Nešić, Ksenija
AU  - Resanović, Radmila
AU  - Jakić-Dimić, Dobrila
AU  - Nešić, Vladimir
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/792
AB  - Experiment was conducted on 160 one-day-old broiler chicks 'Ross' proveniention during 21 day. This research was done with the aim to investigate performances of broilers exposed to the relatively small amount of T-2 toxin (2 mg/kg) and the possibility of prevention and/or alleviating its adverse effects by using various feed additives. Results showed negative consequences of T-2 toxin on body weight, weight gain, feed: gain ratio. All used feed additives in the concentration of 0.2% in feed: inorganic (Minazel-plus, Mz) and organic (Mycosorb, Ms) adsorbents, as well as mixed adsorbent (Mycofix-plus, Mf) mostly alleviated negative effects of T-2 toxin.
AB  - Rezultati istraživanja, koje je urađeno sa ciljem da se ispitaju proizvodni rezultati brojlera izloženih relativno malim količinama T-2 toksina (2 mg/kg), kao i i mogućnost sprečavanja i/ili ublažavanje njegovih štetnih posledica posledice upotrebom različitih dodataka hrani za životinje, pokazali su negativne posledice T-2 toksina na telesnu masu, prirast i konverziju hrane. Svi primenjeni dodaci u koncentraciji od 0,2% u hrani: neorganski (Minazel plus, MZ), organski (Mycosorb, MS), kao i mešoviti adsorbens (Mycofix plus, MF) najvećim delom su ublažili negativne efekte T-2 toksina. Pa ipak, kao najefikasniji pokazao se mešoviti adsorbent. Naime, očigledno je da tokom svog metabolizma BBSH 797, kao sastavni deo Mycofix-a, proizvodi enzime de-epoksidaze koji degradiraju trihotecene selektivnim uništavanjem njihove toksične 12,13-epoksi grupe. Kako je poznato da je 12,13-epoksi prsten trihotecena odgovoran za njegovu toksičnost, dodavanje specifičnih enzima (de-epoksidaza) posledično dovodi i do značajnog smanjenja toksičnosti. Na ovaj način je mešoviti adsorbent najviše ublažio štetno dejstvo T-2 toksina na proizvodne rezultate brojlera.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Efficiency of various feed additives on the performance of broilers treated with T-2 toxin
T1  - Ispitivanje efikasnosti različitih dodataka hrani za životinje na proizvodne rezultate brojlera tretiranih T-2 toksinom
VL  - 27
IS  - 3
SP  - 705
EP  - 711
DO  - 10.2298/BAH1103705N
ER  - 

@article{
author = "Nešić, Ksenija and Resanović, Radmila and Jakić-Dimić, Dobrila and Nešić, Vladimir",
year = "2011",
abstract = "Experiment was conducted on 160 one-day-old broiler chicks 'Ross' proveniention during 21 day. This research was done with the aim to investigate performances of broilers exposed to the relatively small amount of T-2 toxin (2 mg/kg) and the possibility of prevention and/or alleviating its adverse effects by using various feed additives. Results showed negative consequences of T-2 toxin on body weight, weight gain, feed: gain ratio. All used feed additives in the concentration of 0.2% in feed: inorganic (Minazel-plus, Mz) and organic (Mycosorb, Ms) adsorbents, as well as mixed adsorbent (Mycofix-plus, Mf) mostly alleviated negative effects of T-2 toxin., Rezultati istraživanja, koje je urađeno sa ciljem da se ispitaju proizvodni rezultati brojlera izloženih relativno malim količinama T-2 toksina (2 mg/kg), kao i i mogućnost sprečavanja i/ili ublažavanje njegovih štetnih posledica posledice upotrebom različitih dodataka hrani za životinje, pokazali su negativne posledice T-2 toksina na telesnu masu, prirast i konverziju hrane. Svi primenjeni dodaci u koncentraciji od 0,2% u hrani: neorganski (Minazel plus, MZ), organski (Mycosorb, MS), kao i mešoviti adsorbens (Mycofix plus, MF) najvećim delom su ublažili negativne efekte T-2 toksina. Pa ipak, kao najefikasniji pokazao se mešoviti adsorbent. Naime, očigledno je da tokom svog metabolizma BBSH 797, kao sastavni deo Mycofix-a, proizvodi enzime de-epoksidaze koji degradiraju trihotecene selektivnim uništavanjem njihove toksične 12,13-epoksi grupe. Kako je poznato da je 12,13-epoksi prsten trihotecena odgovoran za njegovu toksičnost, dodavanje specifičnih enzima (de-epoksidaza) posledično dovodi i do značajnog smanjenja toksičnosti. Na ovaj način je mešoviti adsorbent najviše ublažio štetno dejstvo T-2 toksina na proizvodne rezultate brojlera.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Efficiency of various feed additives on the performance of broilers treated with T-2 toxin, Ispitivanje efikasnosti različitih dodataka hrani za životinje na proizvodne rezultate brojlera tretiranih T-2 toksinom",
volume = "27",
number = "3",
pages = "705-711",
doi = "10.2298/BAH1103705N"
}

Nešić, K., Resanović, R., Jakić-Dimić, D.,& Nešić, V.. (2011). Efficiency of various feed additives on the performance of broilers treated with T-2 toxin. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 27(3), 705-711.
https://doi.org/10.2298/BAH1103705N

Nešić K, Resanović R, Jakić-Dimić D, Nešić V. Efficiency of various feed additives on the performance of broilers treated with T-2 toxin. in Biotechnology in Animal Husbandry. 2011;27(3):705-711.
doi:10.2298/BAH1103705N .

Nešić, Ksenija, Resanović, Radmila, Jakić-Dimić, Dobrila, Nešić, Vladimir, "Efficiency of various feed additives on the performance of broilers treated with T-2 toxin" in Biotechnology in Animal Husbandry, 27, no. 3 (2011):705-711,
https://doi.org/10.2298/BAH1103705N . .

TY  - CONF
AU  - Šefer, Dragan
AU  - Stanivuk, Predrag
AU  - Marković, Radmila
AU  - Jakić-Dimić, Dobrila
AU  - Grdović, Svetlana
AU  - Radulović, Stamen
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3393
C3  - 4th International Congress on Food and Nutrition, Istanbul, October 12 -14, 2011, 3rd SAFE Consortium International Congress on Food Safety
T1  - Presence of aflatoxins in the feed for lactating cows in Macva district
SP  - 237
EP  - 237
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3393
ER  - 

@conference{
author = "Šefer, Dragan and Stanivuk, Predrag and Marković, Radmila and Jakić-Dimić, Dobrila and Grdović, Svetlana and Radulović, Stamen",
year = "2011",
journal = "4th International Congress on Food and Nutrition, Istanbul, October 12 -14, 2011, 3rd SAFE Consortium International Congress on Food Safety",
title = "Presence of aflatoxins in the feed for lactating cows in Macva district",
pages = "237-237",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3393"
}

Šefer, D., Stanivuk, P., Marković, R., Jakić-Dimić, D., Grdović, S.,& Radulović, S.. (2011). Presence of aflatoxins in the feed for lactating cows in Macva district. in 4th International Congress on Food and Nutrition, Istanbul, October 12 -14, 2011, 3rd SAFE Consortium International Congress on Food Safety, 237-237.
https://hdl.handle.net/21.15107/rcub_veterinar_3393

Šefer D, Stanivuk P, Marković R, Jakić-Dimić D, Grdović S, Radulović S. Presence of aflatoxins in the feed for lactating cows in Macva district. in 4th International Congress on Food and Nutrition, Istanbul, October 12 -14, 2011, 3rd SAFE Consortium International Congress on Food Safety. 2011;:237-237.
https://hdl.handle.net/21.15107/rcub_veterinar_3393 .

Šefer, Dragan, Stanivuk, Predrag, Marković, Radmila, Jakić-Dimić, Dobrila, Grdović, Svetlana, Radulović, Stamen, "Presence of aflatoxins in the feed for lactating cows in Macva district" in 4th International Congress on Food and Nutrition, Istanbul, October 12 -14, 2011, 3rd SAFE Consortium International Congress on Food Safety (2011):237-237,
https://hdl.handle.net/21.15107/rcub_veterinar_3393 .

TY  - JOUR
AU  - Jakić-Dimić, Dobrila
AU  - Nešić, Ksenija
AU  - Šefer, Dragan
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/777
AB  - Recent mycological and mycotoxicological analysis of many feed samples have shown that this problem is still present. It is approved that mouldy feed can contain various mycotoxins, but in our conditions among the most investigated and usually present are some trichotecenes. Based on laboratory feed inspection of samples sent to be analysed in the Institute of Veterinary Medicine of Serbia in Belgrade it was concluded that fungi and mycotoxins contamination are common. Although detected levels usually do not exceed limits layed by actual legislation, having on mind cumulative effects and possible chronical exposure of animals to their harmful influence, appropriate and competent approach is necessary. In fact, even when direct loses, as consequence of high mortality are not present, indirect loses due to drop of performances and production and higher incidence of other deseases must not be neglected.
AB  - Fusarium plesni preovlađuju u našem regionu. Među mikotoksinima koje proizvode Fusarium plesni, živina je naročito osetljiva na T-2 toksin i DAS koji pripadaju grupi trihotecena. Mlada živina, uzgojna živina, patke i golubovi često su osetljiviji na efekte mikotoksina. Kombinacija više različitih vrsta mikotoksina može da dovede do težih efekata čineći problematičnijim definisanje sigurnih nivoa u hrani. U cilju prevencije problema u vezi sa prisustvom mikotoksina u hrani neophodno je pravilno skladištenje i rukovanje žitaricama i gotovom hranom kako bi se smanjio razvoj plesni i produkcija mikotoksina. Takođe, neophodno je vršiti i redovne analize za detekciju mikotoksina. Dodaci hrani za životinje mogu da se koriste za ublažavanje štetnih efekata nekih mikotoksina, ali treba da budu istraženi kako bi njihova aktivnost bila usmerena na mikotoksin od interesa.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycotoxicoses of poultry caused by trichothecenes
T1  - Mikotoksikoze živine prouzrokovane trihotecenima
VL  - 27
IS  - 3
SP  - 713
EP  - 719
DO  - 10.2298/BAH1103713J
ER  - 

@article{
author = "Jakić-Dimić, Dobrila and Nešić, Ksenija and Šefer, Dragan",
year = "2011",
abstract = "Recent mycological and mycotoxicological analysis of many feed samples have shown that this problem is still present. It is approved that mouldy feed can contain various mycotoxins, but in our conditions among the most investigated and usually present are some trichotecenes. Based on laboratory feed inspection of samples sent to be analysed in the Institute of Veterinary Medicine of Serbia in Belgrade it was concluded that fungi and mycotoxins contamination are common. Although detected levels usually do not exceed limits layed by actual legislation, having on mind cumulative effects and possible chronical exposure of animals to their harmful influence, appropriate and competent approach is necessary. In fact, even when direct loses, as consequence of high mortality are not present, indirect loses due to drop of performances and production and higher incidence of other deseases must not be neglected., Fusarium plesni preovlađuju u našem regionu. Među mikotoksinima koje proizvode Fusarium plesni, živina je naročito osetljiva na T-2 toksin i DAS koji pripadaju grupi trihotecena. Mlada živina, uzgojna živina, patke i golubovi često su osetljiviji na efekte mikotoksina. Kombinacija više različitih vrsta mikotoksina može da dovede do težih efekata čineći problematičnijim definisanje sigurnih nivoa u hrani. U cilju prevencije problema u vezi sa prisustvom mikotoksina u hrani neophodno je pravilno skladištenje i rukovanje žitaricama i gotovom hranom kako bi se smanjio razvoj plesni i produkcija mikotoksina. Takođe, neophodno je vršiti i redovne analize za detekciju mikotoksina. Dodaci hrani za životinje mogu da se koriste za ublažavanje štetnih efekata nekih mikotoksina, ali treba da budu istraženi kako bi njihova aktivnost bila usmerena na mikotoksin od interesa.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycotoxicoses of poultry caused by trichothecenes, Mikotoksikoze živine prouzrokovane trihotecenima",
volume = "27",
number = "3",
pages = "713-719",
doi = "10.2298/BAH1103713J"
}

Jakić-Dimić, D., Nešić, K.,& Šefer, D.. (2011). Mycotoxicoses of poultry caused by trichothecenes. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 27(3), 713-719.
https://doi.org/10.2298/BAH1103713J

Jakić-Dimić D, Nešić K, Šefer D. Mycotoxicoses of poultry caused by trichothecenes. in Biotechnology in Animal Husbandry. 2011;27(3):713-719.
doi:10.2298/BAH1103713J .

Jakić-Dimić, Dobrila, Nešić, Ksenija, Šefer, Dragan, "Mycotoxicoses of poultry caused by trichothecenes" in Biotechnology in Animal Husbandry, 27, no. 3 (2011):713-719,
https://doi.org/10.2298/BAH1103713J . .

TY  - JOUR
AU  - Šefer, Dragan
AU  - Andonov, A.
AU  - Šobajić, S.
AU  - Marković, Radmila
AU  - Radulović, Stamen
AU  - Jakić-Dimić, Dobrila
AU  - Petrujkić, Branko
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/785
AB  - In order to investigate the effects of omega (n) 3 fatty acids on egg quality a group-control trial was organized. Trial lasted 40 days and was performed in production conditions. Total number of 1264 laying hens of Lohman Brown classic provenience were used and randomly allotted into one of four groups by 316 hens each (C-control, I experimental, II experimental and III experimental). All groups of hens were fed diets of standard ingredients and chemical composition, while feed of I, II and III experimental group of hens was supplemented with micro algae Schizochytrium spp. (DHA Gold®, Martek, USA) as a source of n- 3 fatty acids in amount of 0.5, 0.7 and 1.0 % respectively. In order to investigate the influence of micro algae Schizochytrium spp. feed supplementation on egg quality, from the aspect of content and ratio of fatty acids, random egg samples (n=10) were taken at the 10th, 20th and 40th day of the trial from each group. Egg yolk fatty acid content was determined by gas chromatography on Varian 1400 with flame ionization detector (FID detector) (on a packed column 20% LAC-3R-728 Chromosorb WAW 80-100 mesh). Significant differences in egg yolk fatty acid content as well as their ratio were determined between treatment groups. Positive influence of supplemented micro algae preparation was observed. Highest content of n-3 poly unsaturated fatty acids (PUFA n-3) was determined in egg yolks of III experimental group while lowest content was determined in egg yolks of control group of hens. Micro algae feed supplementation, at the end of the trial period, positively affected egg yolk PUFA n-6/PUFA n-3 ratio, since determined ratio of 4.24 in III experimental group was more desirable than 12.27 determined in a control group. Marine algae (Schizochytrium spp.) feed supplementation in laying hens in amounts above 0.5% significantly affected egg yolk fatty acid composition as well as ratio of n-6/n-3 PUFA. These findings support its use from nutritive, medical and economic point of view.
AB  - Da bi se ispitao uticaj omega -3 masnih kiselina na kvalitet jaja postavljen je ogled sa tri eksperimentalne i jednom kontrolnom grupom. Ogled je trajao 40 dana i sproveden je u proizvodnim uslovima. Ukupan broj od 1264 kokoši nosilja Lohmann Brown classic provenijence je slučajno raspoređen u četiri grupe po 316 nosilja u svakoj (C-kontrola, I eksperimentalna, II eksperimentalna i III eksperimentalna). Sve grupe su hranjene smešama standardnog sastava, dok su eksperimentalne grupe I, II, III hranjene smešom obogaćenom mikroalgama Schizochytrium spp. (DHA Gold®, Martek, USA) kao izvorom n-3 masnih kiselina u količini od 0,5; 0,7; 1,0. U cilju ispitivanja hrane obogaćene mikroalgom Schizochytrium spp. na kvalitet jaja sa aspekta sadržaja i odnosa masnih kiselina, uzet je slučajan uzorak jaja (n=10) 10., 20., i 40. dana iz svake grupe. Sadržaj masnih kiselina u žumancetu je određen gasnim hromatografom Varian 1400 sa plamen jonizujućim detektorom (FID detector) (na koloni 20% LAC-3R-728 Chromosorb WAW 80-100 mreža). Značajne razlike u sadržaju masnih kiselina u žumancetu kao i njihov odnos su određeni između tretmana. Najveći sadržaj n-3 polinezasićenih masnih kiselina (PUFA n-3) određen je u žumancetu jaja treće eksperimentalne grupe, dok je najmanji sadržaj određen u kontrolnoj grupi. Dodavanje mikro algi smešama na kraju oglednog perioda, pozitivno je uticao na odnos PUFA n-6/PUFA n-3 u žumancetu, pošto je određen odnos od 4,24 u III eksperimentalnoj grupi što je poželjnije od 12,27 koliko je određeno u kontrolnoj grupi. Dodavanje morskih algi (Schizochytrium spp.) u smeše za kokoši nosilje u koncentraciji oko 0,5%, značajno je uticalo na sastav masnih kiselina u žumancetu jajeta kao i na odnos n-6/n-3 PUFA. Ovi rezultati opravdavaju upotrebu algi u ishrani kokoši nosilja sa nutritivne, medicinske i ekonomske tačke gledišta.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile
T1  - Efekat ishrane kokoši nosilja smešama obogaćenim omega-3 masnim kiselinama na sastav masnih kiselina u jajetu
VL  - 27
IS  - 3
SP  - 679
EP  - 686
DO  - 10.2298/BAH1103679S
ER  - 

@article{
author = "Šefer, Dragan and Andonov, A. and Šobajić, S. and Marković, Radmila and Radulović, Stamen and Jakić-Dimić, Dobrila and Petrujkić, Branko",
year = "2011",
abstract = "In order to investigate the effects of omega (n) 3 fatty acids on egg quality a group-control trial was organized. Trial lasted 40 days and was performed in production conditions. Total number of 1264 laying hens of Lohman Brown classic provenience were used and randomly allotted into one of four groups by 316 hens each (C-control, I experimental, II experimental and III experimental). All groups of hens were fed diets of standard ingredients and chemical composition, while feed of I, II and III experimental group of hens was supplemented with micro algae Schizochytrium spp. (DHA Gold®, Martek, USA) as a source of n- 3 fatty acids in amount of 0.5, 0.7 and 1.0 % respectively. In order to investigate the influence of micro algae Schizochytrium spp. feed supplementation on egg quality, from the aspect of content and ratio of fatty acids, random egg samples (n=10) were taken at the 10th, 20th and 40th day of the trial from each group. Egg yolk fatty acid content was determined by gas chromatography on Varian 1400 with flame ionization detector (FID detector) (on a packed column 20% LAC-3R-728 Chromosorb WAW 80-100 mesh). Significant differences in egg yolk fatty acid content as well as their ratio were determined between treatment groups. Positive influence of supplemented micro algae preparation was observed. Highest content of n-3 poly unsaturated fatty acids (PUFA n-3) was determined in egg yolks of III experimental group while lowest content was determined in egg yolks of control group of hens. Micro algae feed supplementation, at the end of the trial period, positively affected egg yolk PUFA n-6/PUFA n-3 ratio, since determined ratio of 4.24 in III experimental group was more desirable than 12.27 determined in a control group. Marine algae (Schizochytrium spp.) feed supplementation in laying hens in amounts above 0.5% significantly affected egg yolk fatty acid composition as well as ratio of n-6/n-3 PUFA. These findings support its use from nutritive, medical and economic point of view., Da bi se ispitao uticaj omega -3 masnih kiselina na kvalitet jaja postavljen je ogled sa tri eksperimentalne i jednom kontrolnom grupom. Ogled je trajao 40 dana i sproveden je u proizvodnim uslovima. Ukupan broj od 1264 kokoši nosilja Lohmann Brown classic provenijence je slučajno raspoređen u četiri grupe po 316 nosilja u svakoj (C-kontrola, I eksperimentalna, II eksperimentalna i III eksperimentalna). Sve grupe su hranjene smešama standardnog sastava, dok su eksperimentalne grupe I, II, III hranjene smešom obogaćenom mikroalgama Schizochytrium spp. (DHA Gold®, Martek, USA) kao izvorom n-3 masnih kiselina u količini od 0,5; 0,7; 1,0. U cilju ispitivanja hrane obogaćene mikroalgom Schizochytrium spp. na kvalitet jaja sa aspekta sadržaja i odnosa masnih kiselina, uzet je slučajan uzorak jaja (n=10) 10., 20., i 40. dana iz svake grupe. Sadržaj masnih kiselina u žumancetu je određen gasnim hromatografom Varian 1400 sa plamen jonizujućim detektorom (FID detector) (na koloni 20% LAC-3R-728 Chromosorb WAW 80-100 mreža). Značajne razlike u sadržaju masnih kiselina u žumancetu kao i njihov odnos su određeni između tretmana. Najveći sadržaj n-3 polinezasićenih masnih kiselina (PUFA n-3) određen je u žumancetu jaja treće eksperimentalne grupe, dok je najmanji sadržaj određen u kontrolnoj grupi. Dodavanje mikro algi smešama na kraju oglednog perioda, pozitivno je uticao na odnos PUFA n-6/PUFA n-3 u žumancetu, pošto je određen odnos od 4,24 u III eksperimentalnoj grupi što je poželjnije od 12,27 koliko je određeno u kontrolnoj grupi. Dodavanje morskih algi (Schizochytrium spp.) u smeše za kokoši nosilje u koncentraciji oko 0,5%, značajno je uticalo na sastav masnih kiselina u žumancetu jajeta kao i na odnos n-6/n-3 PUFA. Ovi rezultati opravdavaju upotrebu algi u ishrani kokoši nosilja sa nutritivne, medicinske i ekonomske tačke gledišta.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile, Efekat ishrane kokoši nosilja smešama obogaćenim omega-3 masnim kiselinama na sastav masnih kiselina u jajetu",
volume = "27",
number = "3",
pages = "679-686",
doi = "10.2298/BAH1103679S"
}

Šefer, D., Andonov, A., Šobajić, S., Marković, R., Radulović, S., Jakić-Dimić, D.,& Petrujkić, B.. (2011). Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 27(3), 679-686.
https://doi.org/10.2298/BAH1103679S

Šefer D, Andonov A, Šobajić S, Marković R, Radulović S, Jakić-Dimić D, Petrujkić B. Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile. in Biotechnology in Animal Husbandry. 2011;27(3):679-686.
doi:10.2298/BAH1103679S .

Šefer, Dragan, Andonov, A., Šobajić, S., Marković, Radmila, Radulović, Stamen, Jakić-Dimić, Dobrila, Petrujkić, Branko, "Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile" in Biotechnology in Animal Husbandry, 27, no. 3 (2011):679-686,
https://doi.org/10.2298/BAH1103679S . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Aleksić, Jelena
AU  - Fratrić, Natalija
AU  - Jakić-Dimić, Dobrila
AU  - Stojić, Velibor
AU  - Pavlović, V.
AU  - Pavlović, Miloš
AU  - Vakanjac, Slobodanka
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/675
AB  - Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu).
AB  - U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite
T1  - Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane
VL  - 60
IS  - 4
SP  - 411
EP  - 423
DO  - 10.2298/AVB1004411G
ER  - 

@article{
author = "Gvozdić, Dragan and Aleksić, Jelena and Fratrić, Natalija and Jakić-Dimić, Dobrila and Stojić, Velibor and Pavlović, V. and Pavlović, Miloš and Vakanjac, Slobodanka",
year = "2010",
abstract = "Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu)., U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite, Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane",
volume = "60",
number = "4",
pages = "411-423",
doi = "10.2298/AVB1004411G"
}

Gvozdić, D., Aleksić, J., Fratrić, N., Jakić-Dimić, D., Stojić, V., Pavlović, V., Pavlović, M.,& Vakanjac, S.. (2010). Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 411-423.
https://doi.org/10.2298/AVB1004411G

Gvozdić D, Aleksić J, Fratrić N, Jakić-Dimić D, Stojić V, Pavlović V, Pavlović M, Vakanjac S. Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd. 2010;60(4):411-423.
doi:10.2298/AVB1004411G .

Gvozdić, Dragan, Aleksić, Jelena, Fratrić, Natalija, Jakić-Dimić, Dobrila, Stojić, Velibor, Pavlović, V., Pavlović, Miloš, Vakanjac, Slobodanka, "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite" in Acta Veterinaria-Beograd, 60, no. 4 (2010):411-423,
https://doi.org/10.2298/AVB1004411G . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Kirovski, Danijela
AU  - Jovanović, M.
AU  - Vujanac, Ivan
AU  - Bojković-Kovačević, Slavica
AU  - Jakić-Dimić, Dobrila
AU  - Prodanović, Radiša
AU  - Stajković, Silvana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/671
AB  - The aim of this study was to estimate the association between body condition and fatty liver in high-yielding dairy cows. One hundred dry Holstein cows were selected. Cows were scored once for body condition during the dry period, puerperium and month 2 of lactation, according to the system provided by Elanco Animal Health Buletin Al 8478. Body condition loss was determined as marked if loss was over 0.7 points between two consecutive phases of cycles and over 1.5 points between puerperium and month 2 of lactation. Liver tissue samples were taken 12 d after calving and tested for lipid content. 50% of cows had mild (3.92 ± 3.33% fat), 33% moderate (19.28 ± 5.18% fat), and 17% severe fatty liver (36.21 ± 4.55% fat). The mean body condition scores were 3.79 ± 0.55 (dry period), 3.18 ± 0.34 (puerperium) and 2.90 ± 0.29 (month 2 of lactation). Marked body condition loss from the dry period to puerperium had 38% of cows, 20% from dry period to month 2 of lactation and 8% from puerperium to month 2 of lactation. Body condition scores out of the physiological range had 39% dry cows, 49 % cows in puerperium and 9% cows at month 2 of lactation. After dividing cows into groups according to fatty liver degree, it was shown that only cows with severe fatty liver were obese during the dry period and that 76.47% of cows from this group had marked condition loss from the dry period to puerperium, 47.06% from dry period to month 2 of lactation and 23.53% from puerperium to month 2 of lactation. These indicate that in cows with severe fatty liver lipomobilisation is intensive and starts around calving, before milk production enhacement. Our results indicate a strong association between body condition and fatty liver in cows, which can be estimated only when body condition is analyzed in details. Besides, our results clearly indicate that body condition is not the only etiological factor that leads to fatty liver in dairy cows.
AB  - Cilj istraživanja u ovom radu je bio da se ispita povezanost telesne kondicije i zamašćenja jetre kod visoko-mlečnih krava. Za ogled je izabrano 100 krava holštajn rase u zasušenju. Telesna kondicija je ocenjena jedanput u zasušenju, puerperijumu i 2. mesecu laktacije prema sistemu Elanco Animal Health Buletin Al 8478. Gubitak u telesnoj kondiciji je označen prekomernim ako je iznosio preko 0,7 poena između dve uzastopne faze ciklusa, odnosno preko 1,5 poena između zasušenja i 2. meseca laktacije. Uzorci jetre su uzeti 12 dana nakon teljenja i ispitani na sadržaj masti. Polovina (50%) krava je imalo blag (3,92±3,33% masti), 33% srednji (19,28±5,18% masti), a 17% krava visok stepen zamašćenja jetre (36,21±4,55% masti). Prosečne OTK krava su iznosile 3,79±0,55 (zasušenje), 3,18±0,34 (puerperijum) i 2,90±0,29 (2. mesec laktacije). Prekomeran gubitak u telesnoj kondiciji između zasušenja i puerperijuma je imalo 38% krava, 20% između zasušenja i 2. meseca laktacije i 8% između puerperijuma i 2. meseca laktacije. Telesnu kondiciju izvan fizioloških granica je imalo 39% krava u zasušenju, 49% krava u puerperijumu i 9% krava u 2. mesecu laktacije. Kada su krave podeljene na osnovu stepena zamašćenja jetre, utvrđeno je da su samo krave sa visokim stepenom zamašćenja jetre bile pregojene u zasušenju, kao i da je kod ove grupe krava u 76,47% slučajeva nastao prekomeran gubitak u telesnoj kondiciji od zasušenja do puerperijuma, u 47,06% od zasušenja do 2. od meseca laktacije i u 23,53% od puerperijuma do 2. meseca laktacije što ukazuje da pojačana lipomobilizacija kod ove grupe krava počinje u periodu oko teljenja vrlo rano, pre nego što nastane značajan porast u proizvodnji mleka. Naši rezultati ukazuju na postojanje značajne povezanosti telesne kodnicije i zamašćenja jetre koja se može utvrditi jedino detaljnom analizom telesne kondicije krava u zapatu. Pored toga, rezultati jasno ukazuju da telesna kondicija nije jedini etiološki faktor u nastanku masne jetre kod krava.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - New insights into body condition score and its association with fatty liver in Holstein dairy cows
T1  - Novi pristup u oceni telesne kondicije i njenom uticaju na zamašćenje jetre kod krava holštajn rase
VL  - 60
IS  - 5-6
SP  - 525
EP  - 540
DO  - 10.2298/AVB1006525S
ER  - 

@article{
author = "Šamanc, Horea and Kirovski, Danijela and Jovanović, M. and Vujanac, Ivan and Bojković-Kovačević, Slavica and Jakić-Dimić, Dobrila and Prodanović, Radiša and Stajković, Silvana",
year = "2010",
abstract = "The aim of this study was to estimate the association between body condition and fatty liver in high-yielding dairy cows. One hundred dry Holstein cows were selected. Cows were scored once for body condition during the dry period, puerperium and month 2 of lactation, according to the system provided by Elanco Animal Health Buletin Al 8478. Body condition loss was determined as marked if loss was over 0.7 points between two consecutive phases of cycles and over 1.5 points between puerperium and month 2 of lactation. Liver tissue samples were taken 12 d after calving and tested for lipid content. 50% of cows had mild (3.92 ± 3.33% fat), 33% moderate (19.28 ± 5.18% fat), and 17% severe fatty liver (36.21 ± 4.55% fat). The mean body condition scores were 3.79 ± 0.55 (dry period), 3.18 ± 0.34 (puerperium) and 2.90 ± 0.29 (month 2 of lactation). Marked body condition loss from the dry period to puerperium had 38% of cows, 20% from dry period to month 2 of lactation and 8% from puerperium to month 2 of lactation. Body condition scores out of the physiological range had 39% dry cows, 49 % cows in puerperium and 9% cows at month 2 of lactation. After dividing cows into groups according to fatty liver degree, it was shown that only cows with severe fatty liver were obese during the dry period and that 76.47% of cows from this group had marked condition loss from the dry period to puerperium, 47.06% from dry period to month 2 of lactation and 23.53% from puerperium to month 2 of lactation. These indicate that in cows with severe fatty liver lipomobilisation is intensive and starts around calving, before milk production enhacement. Our results indicate a strong association between body condition and fatty liver in cows, which can be estimated only when body condition is analyzed in details. Besides, our results clearly indicate that body condition is not the only etiological factor that leads to fatty liver in dairy cows., Cilj istraživanja u ovom radu je bio da se ispita povezanost telesne kondicije i zamašćenja jetre kod visoko-mlečnih krava. Za ogled je izabrano 100 krava holštajn rase u zasušenju. Telesna kondicija je ocenjena jedanput u zasušenju, puerperijumu i 2. mesecu laktacije prema sistemu Elanco Animal Health Buletin Al 8478. Gubitak u telesnoj kondiciji je označen prekomernim ako je iznosio preko 0,7 poena između dve uzastopne faze ciklusa, odnosno preko 1,5 poena između zasušenja i 2. meseca laktacije. Uzorci jetre su uzeti 12 dana nakon teljenja i ispitani na sadržaj masti. Polovina (50%) krava je imalo blag (3,92±3,33% masti), 33% srednji (19,28±5,18% masti), a 17% krava visok stepen zamašćenja jetre (36,21±4,55% masti). Prosečne OTK krava su iznosile 3,79±0,55 (zasušenje), 3,18±0,34 (puerperijum) i 2,90±0,29 (2. mesec laktacije). Prekomeran gubitak u telesnoj kondiciji između zasušenja i puerperijuma je imalo 38% krava, 20% između zasušenja i 2. meseca laktacije i 8% između puerperijuma i 2. meseca laktacije. Telesnu kondiciju izvan fizioloških granica je imalo 39% krava u zasušenju, 49% krava u puerperijumu i 9% krava u 2. mesecu laktacije. Kada su krave podeljene na osnovu stepena zamašćenja jetre, utvrđeno je da su samo krave sa visokim stepenom zamašćenja jetre bile pregojene u zasušenju, kao i da je kod ove grupe krava u 76,47% slučajeva nastao prekomeran gubitak u telesnoj kondiciji od zasušenja do puerperijuma, u 47,06% od zasušenja do 2. od meseca laktacije i u 23,53% od puerperijuma do 2. meseca laktacije što ukazuje da pojačana lipomobilizacija kod ove grupe krava počinje u periodu oko teljenja vrlo rano, pre nego što nastane značajan porast u proizvodnji mleka. Naši rezultati ukazuju na postojanje značajne povezanosti telesne kodnicije i zamašćenja jetre koja se može utvrditi jedino detaljnom analizom telesne kondicije krava u zapatu. Pored toga, rezultati jasno ukazuju da telesna kondicija nije jedini etiološki faktor u nastanku masne jetre kod krava.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "New insights into body condition score and its association with fatty liver in Holstein dairy cows, Novi pristup u oceni telesne kondicije i njenom uticaju na zamašćenje jetre kod krava holštajn rase",
volume = "60",
number = "5-6",
pages = "525-540",
doi = "10.2298/AVB1006525S"
}

Šamanc, H., Kirovski, D., Jovanović, M., Vujanac, I., Bojković-Kovačević, S., Jakić-Dimić, D., Prodanović, R.,& Stajković, S.. (2010). New insights into body condition score and its association with fatty liver in Holstein dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 525-540.
https://doi.org/10.2298/AVB1006525S

Šamanc H, Kirovski D, Jovanović M, Vujanac I, Bojković-Kovačević S, Jakić-Dimić D, Prodanović R, Stajković S. New insights into body condition score and its association with fatty liver in Holstein dairy cows. in Acta Veterinaria-Beograd. 2010;60(5-6):525-540.
doi:10.2298/AVB1006525S .

Šamanc, Horea, Kirovski, Danijela, Jovanović, M., Vujanac, Ivan, Bojković-Kovačević, Slavica, Jakić-Dimić, Dobrila, Prodanović, Radiša, Stajković, Silvana, "New insights into body condition score and its association with fatty liver in Holstein dairy cows" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):525-540,
https://doi.org/10.2298/AVB1006525S . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Stojanović, Dragica
AU  - Žutić, Milenko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/672
AB  - Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype.
AB  - Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens
T1  - Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena
VL  - 60
IS  - 5-6
SP  - 497
EP  - 506
DO  - 10.2298/AVB1006497Z
ER  - 

@article{
author = "Žutić, Jadranka and Ašanin, Jelena and Mišić, Dušan and Jakić-Dimić, Dobrila and Milić, Nenad and Ašanin, Ružica and Stojanović, Dragica and Žutić, Milenko",
year = "2010",
abstract = "Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype., Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens, Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena",
volume = "60",
number = "5-6",
pages = "497-506",
doi = "10.2298/AVB1006497Z"
}

Žutić, J., Ašanin, J., Mišić, D., Jakić-Dimić, D., Milić, N., Ašanin, R., Stojanović, D.,& Žutić, M.. (2010). Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 497-506.
https://doi.org/10.2298/AVB1006497Z

Žutić J, Ašanin J, Mišić D, Jakić-Dimić D, Milić N, Ašanin R, Stojanović D, Žutić M. Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd. 2010;60(5-6):497-506.
doi:10.2298/AVB1006497Z .

Žutić, Jadranka, Ašanin, Jelena, Mišić, Dušan, Jakić-Dimić, Dobrila, Milić, Nenad, Ašanin, Ružica, Stojanović, Dragica, Žutić, Milenko, "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):497-506,
https://doi.org/10.2298/AVB1006497Z . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Jakić-Dimić, Dobrila
AU  - Vujanac, Ivan
AU  - Kureljušić, Branislav
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/726
AB  - The objective of the investigations was to establish the validity of certain metabolic and endocrine indicators of energy metabolism in comparison with the body condition of cows during the final stage of pregnancy and the early stage of lactation. For the investigations, three groups of eight cows each were chosen during the dry period (15 days before the expected date of calving), in early puerperium (15 days after calving) and on the 100th day of lactation. All the animals included in the experiment were primipara or at their second delivery. The body condition of the cows in advanced pregnancy and lactation was evaluated according to the Elanco Animal Health Bulletin AI 8478 system. Concentrations of glucose and beta-hydroxybutyric acid (BHB) were determined in samples of fresh blood, and concentrations of total bilirubin, urea, triiodothyronine and thyroxine in samples of blood serum. The blood samples were taken during the dry period, in early puerperium, and on the 100th day of lactation. On the grounds of the obtained results, a high degree of correlation was determined between the body condition of cows in early puerperium and BHB concentrations in blood established 15 days prior to calving (r = 0.898; p  lt  0.01). Contrary to this, a significant correlation was established between the body condition of cows in the dry period and the concentration of total bilirubin in cow blood 15 days after calving (r = 0.603; p  lt  0.05). Furthermore, a high degree of correlation was established between the difference in the body condition of cows in the dry period and in early puerperium (X = 0.49 ± 0.21 pints) and BHB concentration in the blood of cows prior to calving (r = 0.800; p  lt  0.02). It can be concluded on the grounds of the obtained results that changes in the body condition of cows that occur in the peripartal period are in significant correlation with BHB concentration in the blood of cows in the dry period and the concentration of total bilirubin in early puerperium. .
AB  - Cilj istraživanja bio je da se utvrdi validnost nekih metaboličkih i endokrinih pokazatelja energetskog metabolizma u poređenju sa telesnom kondicijom krava tokom poslednje faze graviditeta i rane faze laktacije. Za ispitivanje su odabrane tri grupe od po osam krava u periodu zasušenja (15 dana pre očekivanog termina teljenja), ranom puerperijumu (15 dana nakon teljenja) i 100. dana laktacije. Sve životinje uključene u ogled bile su prvotelkinje i drugotelkinje. Telesna kondicija krava u visokom graviditetu i laktaciji je ocenjivana prema sistemu Elanco Animal Health Buletin AI 8478. U uzorcima sveže krvi određivana je koncentracija glukoze i beta-hidroksi buterne kiseline (BHB), a u uzorcima krvnog seruma koncentracija ukupnog bilirubina, uree, trijodtironina i tiroksina. Uzorci krvi uzimani su u zasušenju, ranom puerperijumu i 100. dana laktacije. Na osnovu dobijenih rezultata visok stepen korelacije utvrđen je između telesne kondicije krava u ranom puerperijumu i koncentracije BHB u krvi 15 dana pre teljenja (r = 0,898; p  lt  0,01). Nasuprot tome, značajna korelacija je utvrđena između telesne kondicije u zasušenju i koncentracije ukupnog bilirubina u krvi krava 15 dana posle teljenja (r = 0,603; p  lt  0,05). Dodatno je ustanovljen visok stepen korelacije između razlike u telesnoj kondiciji krava u zasušenju i ranom puerperijumu (X = 0,49 ± 0,21 poena) i koncentracije BHB u krvi krava pre teljenja (r = 0,800; p  lt  0,02). Na osnovu dobijenih rezultata može se zaključiti da su promene telesne kondicije krava koje nastaju u peripartalnom periodu u značajnoj korelaciji sa koncentracijom BHB u krvi krava u zasušenju i koncentracijom ukupnog bilirubina u ranom puerperijumu. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Body condition and indicators of energy status of cows in advanced gravidity and early stage of lactation
T1  - Telesna kondicija i pokazatelji energetskog statusa krava u visokom graviditetu i ranoj fazi laktacije
VL  - 64
IS  - 1-2
SP  - 43
EP  - 52
DO  - 10.2298/VETGL1002043P
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Jakić-Dimić, Dobrila and Vujanac, Ivan and Kureljušić, Branislav",
year = "2010",
abstract = "The objective of the investigations was to establish the validity of certain metabolic and endocrine indicators of energy metabolism in comparison with the body condition of cows during the final stage of pregnancy and the early stage of lactation. For the investigations, three groups of eight cows each were chosen during the dry period (15 days before the expected date of calving), in early puerperium (15 days after calving) and on the 100th day of lactation. All the animals included in the experiment were primipara or at their second delivery. The body condition of the cows in advanced pregnancy and lactation was evaluated according to the Elanco Animal Health Bulletin AI 8478 system. Concentrations of glucose and beta-hydroxybutyric acid (BHB) were determined in samples of fresh blood, and concentrations of total bilirubin, urea, triiodothyronine and thyroxine in samples of blood serum. The blood samples were taken during the dry period, in early puerperium, and on the 100th day of lactation. On the grounds of the obtained results, a high degree of correlation was determined between the body condition of cows in early puerperium and BHB concentrations in blood established 15 days prior to calving (r = 0.898; p  lt  0.01). Contrary to this, a significant correlation was established between the body condition of cows in the dry period and the concentration of total bilirubin in cow blood 15 days after calving (r = 0.603; p  lt  0.05). Furthermore, a high degree of correlation was established between the difference in the body condition of cows in the dry period and in early puerperium (X = 0.49 ± 0.21 pints) and BHB concentration in the blood of cows prior to calving (r = 0.800; p  lt  0.02). It can be concluded on the grounds of the obtained results that changes in the body condition of cows that occur in the peripartal period are in significant correlation with BHB concentration in the blood of cows in the dry period and the concentration of total bilirubin in early puerperium. ., Cilj istraživanja bio je da se utvrdi validnost nekih metaboličkih i endokrinih pokazatelja energetskog metabolizma u poređenju sa telesnom kondicijom krava tokom poslednje faze graviditeta i rane faze laktacije. Za ispitivanje su odabrane tri grupe od po osam krava u periodu zasušenja (15 dana pre očekivanog termina teljenja), ranom puerperijumu (15 dana nakon teljenja) i 100. dana laktacije. Sve životinje uključene u ogled bile su prvotelkinje i drugotelkinje. Telesna kondicija krava u visokom graviditetu i laktaciji je ocenjivana prema sistemu Elanco Animal Health Buletin AI 8478. U uzorcima sveže krvi određivana je koncentracija glukoze i beta-hidroksi buterne kiseline (BHB), a u uzorcima krvnog seruma koncentracija ukupnog bilirubina, uree, trijodtironina i tiroksina. Uzorci krvi uzimani su u zasušenju, ranom puerperijumu i 100. dana laktacije. Na osnovu dobijenih rezultata visok stepen korelacije utvrđen je između telesne kondicije krava u ranom puerperijumu i koncentracije BHB u krvi 15 dana pre teljenja (r = 0,898; p  lt  0,01). Nasuprot tome, značajna korelacija je utvrđena između telesne kondicije u zasušenju i koncentracije ukupnog bilirubina u krvi krava 15 dana posle teljenja (r = 0,603; p  lt  0,05). Dodatno je ustanovljen visok stepen korelacije između razlike u telesnoj kondiciji krava u zasušenju i ranom puerperijumu (X = 0,49 ± 0,21 poena) i koncentracije BHB u krvi krava pre teljenja (r = 0,800; p  lt  0,02). Na osnovu dobijenih rezultata može se zaključiti da su promene telesne kondicije krava koje nastaju u peripartalnom periodu u značajnoj korelaciji sa koncentracijom BHB u krvi krava u zasušenju i koncentracijom ukupnog bilirubina u ranom puerperijumu. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Body condition and indicators of energy status of cows in advanced gravidity and early stage of lactation, Telesna kondicija i pokazatelji energetskog statusa krava u visokom graviditetu i ranoj fazi laktacije",
volume = "64",
number = "1-2",
pages = "43-52",
doi = "10.2298/VETGL1002043P"
}

Prodanović, R., Kirovski, D., Jakić-Dimić, D., Vujanac, I.,& Kureljušić, B.. (2010). Body condition and indicators of energy status of cows in advanced gravidity and early stage of lactation. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1-2), 43-52.
https://doi.org/10.2298/VETGL1002043P

Prodanović R, Kirovski D, Jakić-Dimić D, Vujanac I, Kureljušić B. Body condition and indicators of energy status of cows in advanced gravidity and early stage of lactation. in Veterinarski Glasnik. 2010;64(1-2):43-52.
doi:10.2298/VETGL1002043P .

Prodanović, Radiša, Kirovski, Danijela, Jakić-Dimić, Dobrila, Vujanac, Ivan, Kureljušić, Branislav, "Body condition and indicators of energy status of cows in advanced gravidity and early stage of lactation" in Veterinarski Glasnik, 64, no. 1-2 (2010):43-52,
https://doi.org/10.2298/VETGL1002043P . .

TY  - JOUR
AU  - Ašanin, Ružica
AU  - Žutić, Milenko
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Žutić, Jadranka
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/592
AB  - The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. .
AB  - Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets
T1  - Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi
VL  - 63
IS  - 5-6
SP  - 311
EP  - 320
DO  - 10.2298/VETGL0906311A
ER  - 

@article{
author = "Ašanin, Ružica and Žutić, Milenko and Ašanin, Jelena and Mišić, Dušan and Žutić, Jadranka and Jakić-Dimić, Dobrila and Milić, Nenad and Nišavić, Jakov",
year = "2009",
abstract = "The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. ., Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets, Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi",
volume = "63",
number = "5-6",
pages = "311-320",
doi = "10.2298/VETGL0906311A"
}

Ašanin, R., Žutić, M., Ašanin, J., Mišić, D., Žutić, J., Jakić-Dimić, D., Milić, N.,& Nišavić, J.. (2009). The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 311-320.
https://doi.org/10.2298/VETGL0906311A

Ašanin R, Žutić M, Ašanin J, Mišić D, Žutić J, Jakić-Dimić D, Milić N, Nišavić J. The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik. 2009;63(5-6):311-320.
doi:10.2298/VETGL0906311A .

Ašanin, Ružica, Žutić, Milenko, Ašanin, Jelena, Mišić, Dušan, Žutić, Jadranka, Jakić-Dimić, Dobrila, Milić, Nenad, Nišavić, Jakov, "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets" in Veterinarski Glasnik, 63, no. 5-6 (2009):311-320,
https://doi.org/10.2298/VETGL0906311A . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Pušić, M.
AU  - Jakić-Dimić, Dobrila
AU  - Vujanac, Ivan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/655
AB  - The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05).
AB  - Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving
T1  - Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja
VL  - 59
IS  - 1
SP  - 17
EP  - 23
DO  - 10.2298/AVB0901017S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Pušić, M. and Jakić-Dimić, Dobrila and Vujanac, Ivan",
year = "2009",
abstract = "The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05)., Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving, Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja",
volume = "59",
number = "1",
pages = "17-23",
doi = "10.2298/AVB0901017S"
}

Šamanc, H., Stojić, V., Kirovski, D., Pušić, M., Jakić-Dimić, D.,& Vujanac, I.. (2009). Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 17-23.
https://doi.org/10.2298/AVB0901017S

Šamanc H, Stojić V, Kirovski D, Pušić M, Jakić-Dimić D, Vujanac I. Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd. 2009;59(1):17-23.
doi:10.2298/AVB0901017S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Pušić, M., Jakić-Dimić, Dobrila, Vujanac, Ivan, "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving" in Acta Veterinaria-Beograd, 59, no. 1 (2009):17-23,
https://doi.org/10.2298/AVB0901017S . .