TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Mirčeta, Jovan
AU  - Djurdjević, Biljana
AU  - Lazić, Sava
AU  - Aleksić-Kovačević, Sanja
AU  - Petrović, Jelena
AU  - Polaček, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2941
AB  - African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.
PB  - MDPI
T2  - Pathogens
T1  - African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia
VL  - 12
IS  - 5
SP  - 691
DO  - 10.3390/pathogens12050691
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Mirčeta, Jovan and Djurdjević, Biljana and Lazić, Sava and Aleksić-Kovačević, Sanja and Petrović, Jelena and Polaček, Vladimir",
year = "2023",
abstract = "African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.",
publisher = "MDPI",
journal = "Pathogens",
title = "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia",
volume = "12",
number = "5",
pages = "691",
doi = "10.3390/pathogens12050691"
}

Prodanov-Radulović, J., Mirčeta, J., Djurdjević, B., Lazić, S., Aleksić-Kovačević, S., Petrović, J.,& Polaček, V.. (2023). African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens
MDPI., 12(5), 691.
https://doi.org/10.3390/pathogens12050691

Prodanov-Radulović J, Mirčeta J, Djurdjević B, Lazić S, Aleksić-Kovačević S, Petrović J, Polaček V. African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens. 2023;12(5):691.
doi:10.3390/pathogens12050691 .

Prodanov-Radulović, Jasna, Mirčeta, Jovan, Djurdjević, Biljana, Lazić, Sava, Aleksić-Kovačević, Sanja, Petrović, Jelena, Polaček, Vladimir, "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia" in Pathogens, 12, no. 5 (2023):691,
https://doi.org/10.3390/pathogens12050691 . .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Petrović, Tamaš
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2706
AB  - In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.
PB  - MDPI
T2  - Animals
T1  - Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
VL  - 13
IS  - 4
SP  - 700
DO  - 10.3390/ani13040700
ER  - 

@article{
author = "Đurđević, Biljana and Polaček, Vladimir and Pajić, Marko and Petrović, Tamaš and Vučićević, Ivana and Vidanović, Dejan and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.",
publisher = "MDPI",
journal = "Animals",
title = "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia",
volume = "13",
number = "4",
pages = "700",
doi = "10.3390/ani13040700"
}

Đurđević, B., Polaček, V., Pajić, M., Petrović, T., Vučićević, I., Vidanović, D.,& Aleksić-Kovačević, S.. (2023). Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals
MDPI., 13(4), 700.
https://doi.org/10.3390/ani13040700

Đurđević B, Polaček V, Pajić M, Petrović T, Vučićević I, Vidanović D, Aleksić-Kovačević S. Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals. 2023;13(4):700.
doi:10.3390/ani13040700 .

Đurđević, Biljana, Polaček, Vladimir, Pajić, Marko, Petrović, Tamaš, Vučićević, Ivana, Vidanović, Dejan, Aleksić-Kovačević, Sanja, "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia" in Animals, 13, no. 4 (2023):700,
https://doi.org/10.3390/ani13040700 . .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Vučićević, Ivana
AU  - Bogunović, Danica
AU  - Pajić, Marko
AU  - Vejnović, Branislav
AU  - Prodanov-Radulović, Jasna
AU  - Polaček, Vladimir
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2786
AB  - Background The Sarconema eurycerca heart infection may be one of the main causes of death in swans and geese due to
severe heart lesions. To date, there have been no reports of Sarconema eurycerca in mute swans (Cygnus olor) in Serbia and
this parasite has never been morphologically characterized in wild birds from this region.
Methods In spring 2021, eight dead mute swans (Cygnus olor) were collected during avian influenza surveillance on the territory
of the Province of Vojvodina and submitted to Scientific Veterinary Institute “Novi Sad” to determine the cause of death.
The presence of highly pathogenic avian influenza subtype H5N1 was determined in all swans using molecular methods.
Results Necropsy of the swans revealed changes characteristic for highly pathogenic avian influenza infection, such as severe
haemorrhages and necrosis in different organs. Three of the eight swans showed massive subepicardial haemorrhages and
myocarditis with intralesional 2–4 cm long whitish roundworms. Histopathologically, haemorrhages and many adult heart
worms were observed in the myocardium. Based on morphological features of parasites, infection by Sarconema eurycerca
was concluded.
Conclusion According to the available scientific literature, the presence of heart roundworms in swans has not yet been
confirmed in our country. This report is the first published case of cardiac filariasis associated with S. eurycerca infection
in mute swans in the Republic of Serbia.
PB  - Springer
T2  - Acta Parasitologica
T1  - First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia
VL  - 67
IS  - 4
SP  - 1773
EP  - 1777
DO  - 10.1007/s11686-022-00616-3
ER  - 

@article{
author = "Đurđević, Biljana and Vučićević, Ivana and Bogunović, Danica and Pajić, Marko and Vejnović, Branislav and Prodanov-Radulović, Jasna and Polaček, Vladimir",
year = "2022",
abstract = "Background The Sarconema eurycerca heart infection may be one of the main causes of death in swans and geese due to
severe heart lesions. To date, there have been no reports of Sarconema eurycerca in mute swans (Cygnus olor) in Serbia and
this parasite has never been morphologically characterized in wild birds from this region.
Methods In spring 2021, eight dead mute swans (Cygnus olor) were collected during avian influenza surveillance on the territory
of the Province of Vojvodina and submitted to Scientific Veterinary Institute “Novi Sad” to determine the cause of death.
The presence of highly pathogenic avian influenza subtype H5N1 was determined in all swans using molecular methods.
Results Necropsy of the swans revealed changes characteristic for highly pathogenic avian influenza infection, such as severe
haemorrhages and necrosis in different organs. Three of the eight swans showed massive subepicardial haemorrhages and
myocarditis with intralesional 2–4 cm long whitish roundworms. Histopathologically, haemorrhages and many adult heart
worms were observed in the myocardium. Based on morphological features of parasites, infection by Sarconema eurycerca
was concluded.
Conclusion According to the available scientific literature, the presence of heart roundworms in swans has not yet been
confirmed in our country. This report is the first published case of cardiac filariasis associated with S. eurycerca infection
in mute swans in the Republic of Serbia.",
publisher = "Springer",
journal = "Acta Parasitologica",
title = "First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia",
volume = "67",
number = "4",
pages = "1773-1777",
doi = "10.1007/s11686-022-00616-3"
}

Đurđević, B., Vučićević, I., Bogunović, D., Pajić, M., Vejnović, B., Prodanov-Radulović, J.,& Polaček, V.. (2022). First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia. in Acta Parasitologica
Springer., 67(4), 1773-1777.
https://doi.org/10.1007/s11686-022-00616-3

Đurđević B, Vučićević I, Bogunović D, Pajić M, Vejnović B, Prodanov-Radulović J, Polaček V. First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia. in Acta Parasitologica. 2022;67(4):1773-1777.
doi:10.1007/s11686-022-00616-3 .

Đurđević, Biljana, Vučićević, Ivana, Bogunović, Danica, Pajić, Marko, Vejnović, Branislav, Prodanov-Radulović, Jasna, Polaček, Vladimir, "First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia" in Acta Parasitologica, 67, no. 4 (2022):1773-1777,
https://doi.org/10.1007/s11686-022-00616-3 . .

TY  - JOUR
AU  - Nešković, Milijana
AU  - Ristić, Bojan
AU  - Došenović, Rade
AU  - Grubač, Siniša
AU  - Petrović, Tamaš
AU  - Prodanov-Radulović, Jasna
AU  - Polaček, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2210
AB  - The fi rst confi rmed case of African Swine Fever (ASF) in Serbia occurred in 2019. Since then, numerous outbreaks in domestic pigs and wild boars have been reported. Until April 2021, all the detected ASF cases were in backyard pigs. Beside backyard and smallholders farming systems as a dominant pig production system in Serbia, large commercial pig farms can also be found, located mostly in villages. In the beginning of April 2021, a large commercial farrow-to-fi nish pig farm in Serbia with almost 19,000 animals was affected with the ASF virus. The pig farm analysed in this paper is located in an area where ASF was confi rmed earlier in both backyard and wild boars. In this study, we describe the overall epidemiological course of the ASF outbreak. Epidemiological investigation using a hypothesis-based approach was conducted in order to reconstruct the disease course until the offi cial notifi cation. All the available data from the farm were analysed with the aim to determine the high-risk period (HRP). Some of the key points to consider when it comes to the sources of infection and entry route of ASF are the following: contamination of the area in the immediate vicinity of the farm, risky human activities, irregularities and some omissions in the external farm biosecurity and immediate proximity of the city waste-yard where the communal waste is disposed of. It was concluded that when commercial pig farms are surrounded by villages with a large number of backyards, hence the anthropogenic factor is the key risk factor for ASF spreading
AB  - Prvi slučaj afričke kuge svinja (AKS) u Srbiji je dijagnostikovan 2019. godine. Od tada, beleži se pojava bolesti u populaciji domać ih i divljih svinja. Sve do aprila 2021. godine žarišta AKS su bila lokalizovana u populaciji domaćih svinja u seoskim dvorištima. Pored seoskih gazdinstava i porodičnih farmi kao dominantnog načina proizvodnje, u Srbiji se nalaze i velike komercijalne farme svinja, uglavnom oko seoskih naselja. Početkom aprila 2021. godine, u komercijalnoj farma svinja, proizvodnog kapaciteta oko 19000 jedinki, potvrdjena je infekcija virusom AKS. U radu je sa epizootiološkog aspekta, hronološki opisan tok infekcije AKS. Epizootiološko istraživanje je zasnovano na hipotezama u cilju utvrdjivanja potencijalnih puteva unošenja AKS. Analizirani su dostupni farmski podaci kako bi se utvrdio vremenski period visokog rizika (HRP). Farma svinja se nalazi u području gde je u prethodnom periodu potvrđena AKS u seoskim gazdinstvima, kao i u populaciji divljih svinja. Kontaminacija ambijenta u neposrednoj blizini farme, visoko rizične aktivnosti humane populacije u okruženju (brojna seoska gazdinstva), postojanje pojedinih aktivnosti visokog rizika u realizaciji eksterne biosigurnosti i neposredna blizina gradske deponije gde se odlaže komunalni otpad, su svakako ključni momenti kada se razmatraju izvori infekcije i put unošenja AKS. Zaključeno je da u uslovima kada su komercijalne farme okružene selima, u kojima su dominira uzgoj domaćih svinja u seoskim dvorištima, ljudske aktivnosti predstavljaju ključni faktor rizika za širenje AKS.
T2  - Acta Veterinaria-Beograd
T2  - Acta Veterinaria-Beograd
T1  - African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia
T1  - Istraživanje pojave afričke kuge svinja na velikoj komercijalnoj farmi svinja u Srbiji
VL  - 71
IS  - 2
SP  - 219
EP  - 229
DO  - 10.2478/acve-2021-0019
ER  - 

@article{
author = "Nešković, Milijana and Ristić, Bojan and Došenović, Rade and Grubač, Siniša and Petrović, Tamaš and Prodanov-Radulović, Jasna and Polaček, Vladimir",
year = "2021",
abstract = "The fi rst confi rmed case of African Swine Fever (ASF) in Serbia occurred in 2019. Since then, numerous outbreaks in domestic pigs and wild boars have been reported. Until April 2021, all the detected ASF cases were in backyard pigs. Beside backyard and smallholders farming systems as a dominant pig production system in Serbia, large commercial pig farms can also be found, located mostly in villages. In the beginning of April 2021, a large commercial farrow-to-fi nish pig farm in Serbia with almost 19,000 animals was affected with the ASF virus. The pig farm analysed in this paper is located in an area where ASF was confi rmed earlier in both backyard and wild boars. In this study, we describe the overall epidemiological course of the ASF outbreak. Epidemiological investigation using a hypothesis-based approach was conducted in order to reconstruct the disease course until the offi cial notifi cation. All the available data from the farm were analysed with the aim to determine the high-risk period (HRP). Some of the key points to consider when it comes to the sources of infection and entry route of ASF are the following: contamination of the area in the immediate vicinity of the farm, risky human activities, irregularities and some omissions in the external farm biosecurity and immediate proximity of the city waste-yard where the communal waste is disposed of. It was concluded that when commercial pig farms are surrounded by villages with a large number of backyards, hence the anthropogenic factor is the key risk factor for ASF spreading, Prvi slučaj afričke kuge svinja (AKS) u Srbiji je dijagnostikovan 2019. godine. Od tada, beleži se pojava bolesti u populaciji domać ih i divljih svinja. Sve do aprila 2021. godine žarišta AKS su bila lokalizovana u populaciji domaćih svinja u seoskim dvorištima. Pored seoskih gazdinstava i porodičnih farmi kao dominantnog načina proizvodnje, u Srbiji se nalaze i velike komercijalne farme svinja, uglavnom oko seoskih naselja. Početkom aprila 2021. godine, u komercijalnoj farma svinja, proizvodnog kapaciteta oko 19000 jedinki, potvrdjena je infekcija virusom AKS. U radu je sa epizootiološkog aspekta, hronološki opisan tok infekcije AKS. Epizootiološko istraživanje je zasnovano na hipotezama u cilju utvrdjivanja potencijalnih puteva unošenja AKS. Analizirani su dostupni farmski podaci kako bi se utvrdio vremenski period visokog rizika (HRP). Farma svinja se nalazi u području gde je u prethodnom periodu potvrđena AKS u seoskim gazdinstvima, kao i u populaciji divljih svinja. Kontaminacija ambijenta u neposrednoj blizini farme, visoko rizične aktivnosti humane populacije u okruženju (brojna seoska gazdinstva), postojanje pojedinih aktivnosti visokog rizika u realizaciji eksterne biosigurnosti i neposredna blizina gradske deponije gde se odlaže komunalni otpad, su svakako ključni momenti kada se razmatraju izvori infekcije i put unošenja AKS. Zaključeno je da u uslovima kada su komercijalne farme okružene selima, u kojima su dominira uzgoj domaćih svinja u seoskim dvorištima, ljudske aktivnosti predstavljaju ključni faktor rizika za širenje AKS.",
journal = "Acta Veterinaria-Beograd, Acta Veterinaria-Beograd",
title = "African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia, Istraživanje pojave afričke kuge svinja na velikoj komercijalnoj farmi svinja u Srbiji",
volume = "71",
number = "2",
pages = "219-229",
doi = "10.2478/acve-2021-0019"
}

Nešković, M., Ristić, B., Došenović, R., Grubač, S., Petrović, T., Prodanov-Radulović, J.,& Polaček, V.. (2021). African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia. in Acta Veterinaria-Beograd, 71(2), 219-229.
https://doi.org/10.2478/acve-2021-0019

Nešković M, Ristić B, Došenović R, Grubač S, Petrović T, Prodanov-Radulović J, Polaček V. African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia. in Acta Veterinaria-Beograd. 2021;71(2):219-229.
doi:10.2478/acve-2021-0019 .

Nešković, Milijana, Ristić, Bojan, Došenović, Rade, Grubač, Siniša, Petrović, Tamaš, Prodanov-Radulović, Jasna, Polaček, Vladimir, "African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia" in Acta Veterinaria-Beograd, 71, no. 2 (2021):219-229,
https://doi.org/10.2478/acve-2021-0019 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Đurđević, Biljana
AU  - Petrović, Tamaš
AU  - Prodanov-Radulović, Jasna
AU  - Samojlović, Milena
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1892
AB  - The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis.
AB  - Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.
PB  - Scientific Veterinary Institute “Novi Sad”
T2  - Archives of Veterinary Medicine
T1  - Classical swine fever virus detection in fetal swine tissues by immunohistochemistry
T1  - Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode
VL  - 13
IS  - 1
DO  - 10.46784/e-avm.v13i1.235
ER  - 

@article{
author = "Polaček, Vladimir and Đurđević, Biljana and Petrović, Tamaš and Prodanov-Radulović, Jasna and Samojlović, Milena and Vučićević, Ivana and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis., Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.",
publisher = "Scientific Veterinary Institute “Novi Sad”",
journal = "Archives of Veterinary Medicine",
title = "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry, Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode",
volume = "13",
number = "1",
doi = "10.46784/e-avm.v13i1.235"
}

Polaček, V., Đurđević, B., Petrović, T., Prodanov-Radulović, J., Samojlović, M., Vučićević, I.,& Aleksić-Kovačević, S.. (2020). Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”., 13(1).
https://doi.org/10.46784/e-avm.v13i1.235

Polaček V, Đurđević B, Petrović T, Prodanov-Radulović J, Samojlović M, Vučićević I, Aleksić-Kovačević S. Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine. 2020;13(1).
doi:10.46784/e-avm.v13i1.235 .

Polaček, Vladimir, Đurđević, Biljana, Petrović, Tamaš, Prodanov-Radulović, Jasna, Samojlović, Milena, Vučićević, Ivana, Aleksić-Kovačević, Sanja, "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry" in Archives of Veterinary Medicine, 13, no. 1 (2020),
https://doi.org/10.46784/e-avm.v13i1.235 . .

TY  - JOUR
AU  - Pajić, Marko
AU  - Aleksić, Nevenka
AU  - Vejnović, Branislav
AU  - Polaček, Vladimir
AU  - Novakov, Nikolina
AU  - Ostojić-Andrić, Dušica
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1733
AB  - The influence of certain anticoccidial drugs on oxidative stress in broiler chickens infected with Eimeria species was assessed. There were two untreated (uninfected and infected), and three groups infected and treated with anticoccidials. The first treated group (Ro) was given robenidine, the 2nd a herbal anticoccidial (Herb) and the 3rd the combination of robenidine and the herbal anticoccidial (Ro+Herb). All infected groups were on day 14 challenged with oral inoculation of oocysts. The activities of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST), and the concentration of malondialdehyde (MDA) were estimated in blood taken on days 21 and 40. The oocyst numbers were calculated per gram, and chicken body weight and feed conversion ratio (FCR) measured. The activities of CAT, GST and the level of MDA were significantly lower (P<0.05), whilst the activity of SOD was higher in infected chickens treated with anticoccidials (P<0.05) in comparison to those untreated. The most prominent change in the parameters of oxidative stress was recorded in the Ro+Herb group. In chickens treated with anticoccidials body weight was significantly higher (P<0.05), and the FCR and the oocyst counts significantly lower (P<0.05) than in untreated chickens. Oocyst counts were lower in the Ro and Ro+Herb groups than in the Herb group. Our study demonstrated that both anticoccidial substances exerted antioxidant and anticoccidial effects.
PB  - Kafkas Univ, Veteriner Fakultesi Dergisi, Kars
T2  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
T1  - Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species
VL  - 25
IS  - 3
SP  - 379
EP  - 385
DO  - 10.9775/kvfd.2018.21021
ER  - 

@article{
author = "Pajić, Marko and Aleksić, Nevenka and Vejnović, Branislav and Polaček, Vladimir and Novakov, Nikolina and Ostojić-Andrić, Dušica and Stanimirović, Zoran",
year = "2019",
abstract = "The influence of certain anticoccidial drugs on oxidative stress in broiler chickens infected with Eimeria species was assessed. There were two untreated (uninfected and infected), and three groups infected and treated with anticoccidials. The first treated group (Ro) was given robenidine, the 2nd a herbal anticoccidial (Herb) and the 3rd the combination of robenidine and the herbal anticoccidial (Ro+Herb). All infected groups were on day 14 challenged with oral inoculation of oocysts. The activities of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST), and the concentration of malondialdehyde (MDA) were estimated in blood taken on days 21 and 40. The oocyst numbers were calculated per gram, and chicken body weight and feed conversion ratio (FCR) measured. The activities of CAT, GST and the level of MDA were significantly lower (P<0.05), whilst the activity of SOD was higher in infected chickens treated with anticoccidials (P<0.05) in comparison to those untreated. The most prominent change in the parameters of oxidative stress was recorded in the Ro+Herb group. In chickens treated with anticoccidials body weight was significantly higher (P<0.05), and the FCR and the oocyst counts significantly lower (P<0.05) than in untreated chickens. Oocyst counts were lower in the Ro and Ro+Herb groups than in the Herb group. Our study demonstrated that both anticoccidial substances exerted antioxidant and anticoccidial effects.",
publisher = "Kafkas Univ, Veteriner Fakultesi Dergisi, Kars",
journal = "Kafkas Üniversitesi Veteriner Fakültesi Dergisi",
title = "Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species",
volume = "25",
number = "3",
pages = "379-385",
doi = "10.9775/kvfd.2018.21021"
}

Pajić, M., Aleksić, N., Vejnović, B., Polaček, V., Novakov, N., Ostojić-Andrić, D.,& Stanimirović, Z.. (2019). Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi
Kafkas Univ, Veteriner Fakultesi Dergisi, Kars., 25(3), 379-385.
https://doi.org/10.9775/kvfd.2018.21021

Pajić M, Aleksić N, Vejnović B, Polaček V, Novakov N, Ostojić-Andrić D, Stanimirović Z. Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019;25(3):379-385.
doi:10.9775/kvfd.2018.21021 .

Pajić, Marko, Aleksić, Nevenka, Vejnović, Branislav, Polaček, Vladimir, Novakov, Nikolina, Ostojić-Andrić, Dušica, Stanimirović, Zoran, "Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species" in Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25, no. 3 (2019):379-385,
https://doi.org/10.9775/kvfd.2018.21021 . .

TY  - JOUR
AU  - Bozic (Durdevic), Biljana
AU  - Vučićević, Ivana
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Petrović, Tamaš
AU  - Pajić, Marko
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1770
AB  - The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.
PB  - Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo
T2  - Veterinaria Italiana
T1  - Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia
VL  - 55
IS  - 1
SP  - 95
EP  - 101
DO  - 10.12834/VetIt.1463.7919.2
ER  - 

@article{
author = "Bozic (Durdevic), Biljana and Vučićević, Ivana and Polaček, Vladimir and Vasković, Nikola and Petrović, Tamaš and Pajić, Marko and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.",
publisher = "Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo",
journal = "Veterinaria Italiana",
title = "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia",
volume = "55",
number = "1",
pages = "95-101",
doi = "10.12834/VetIt.1463.7919.2"
}

Bozic (Durdevic), B., Vučićević, I., Polaček, V., Vasković, N., Petrović, T., Pajić, M.,& Aleksić-Kovačević, S.. (2019). Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana
Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo., 55(1), 95-101.
https://doi.org/10.12834/VetIt.1463.7919.2

Bozic (Durdevic) B, Vučićević I, Polaček V, Vasković N, Petrović T, Pajić M, Aleksić-Kovačević S. Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana. 2019;55(1):95-101.
doi:10.12834/VetIt.1463.7919.2 .

Bozic (Durdevic), Biljana, Vučićević, Ivana, Polaček, Vladimir, Vasković, Nikola, Petrović, Tamaš, Pajić, Marko, Aleksić-Kovačević, Sanja, "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia" in Veterinaria Italiana, 55, no. 1 (2019):95-101,
https://doi.org/10.12834/VetIt.1463.7919.2 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Vidanović, Dejan
AU  - Božić, B.
AU  - Becskei, Zsolt
AU  - Vučićević, Ivana
AU  - Prodanov-Radulović, Jasna
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1598
AB  - The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
PB  - Faculty of Veterinary Medicine - Skopje
T2  - Macedonian Veterinary Review
T1  - The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis
VL  - 41
IS  - 1
SP  - 47
EP  - 53
DO  - 10.1515/macvetrev-2017-0030
ER  - 

@article{
author = "Polaček, Vladimir and Vidanović, Dejan and Božić, B. and Becskei, Zsolt and Vučićević, Ivana and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.",
publisher = "Faculty of Veterinary Medicine - Skopje",
journal = "Macedonian Veterinary Review",
title = "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis",
volume = "41",
number = "1",
pages = "47-53",
doi = "10.1515/macvetrev-2017-0030"
}

Polaček, V., Vidanović, D., Božić, B., Becskei, Z., Vučićević, I., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review
Faculty of Veterinary Medicine - Skopje., 41(1), 47-53.
https://doi.org/10.1515/macvetrev-2017-0030

Polaček V, Vidanović D, Božić B, Becskei Z, Vučićević I, Prodanov-Radulović J, Aleksić-Kovačević S. The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review. 2018;41(1):47-53.
doi:10.1515/macvetrev-2017-0030 .

Polaček, Vladimir, Vidanović, Dejan, Božić, B., Becskei, Zsolt, Vučićević, Ivana, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis" in Macedonian Veterinary Review, 41, no. 1 (2018):47-53,
https://doi.org/10.1515/macvetrev-2017-0030 . .

TY  - JOUR
AU  - Bozić, Biljana
AU  - Polaček, Vladimir
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Vasković, Nikola
AU  - Prodanov-Radulović, Jasna
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1651
AB  - During the epizootic of highly pathogenic avian influenza subtype H5N8 in Serbia in the winter of 2016-2017, the highest percent of mortality due to this infection was recorded in mute swans (Cygnus olor). Besides mute swans, avian influenza virus subtype H5N8 was also diagnosed in a small number of hens in rural households. Pancreatic tissues from avian influenza H5N8 positive mute swans and hens that died during this outbreak were collected to determine the character of morphological lesions and the distribution of the viral antigen in this organ. Macroscopic examination of the pancreas of mute swans revealed hemorrhages as well as necrosis, while there were no macroscopic visible lesions in the pancreas of infected hens. Despite the different macroscopic finding, microscopic examination of the pancreas of both infected bird species revealed lesions in the form of acute pancreatitis and multifocal acinar necrosis. The viral antigen was abundantly expressed in the cytoplasm and nucleus of necrotic cells, as well as in macrophages in both examined bird species. Immunohistochemical expression of the viral antigen in the pancreas was strongly consistent with histological lesions. According to the above descnbed findings, it could be concluded that highly pathogenic avian influenza virus H5N8 has a high affinity to pancreatic tissue in both mute swans and hens and the distribution and the character of the lesions in the pancreas are similar in both bird species.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8
VL  - 68
IS  - 2
SP  - 217
EP  - 223
DO  - 10.2478/acve-2018-0018
ER  - 

@article{
author = "Bozić, Biljana and Polaček, Vladimir and Vučićević, Ivana and Vidanović, Dejan and Vasković, Nikola and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "During the epizootic of highly pathogenic avian influenza subtype H5N8 in Serbia in the winter of 2016-2017, the highest percent of mortality due to this infection was recorded in mute swans (Cygnus olor). Besides mute swans, avian influenza virus subtype H5N8 was also diagnosed in a small number of hens in rural households. Pancreatic tissues from avian influenza H5N8 positive mute swans and hens that died during this outbreak were collected to determine the character of morphological lesions and the distribution of the viral antigen in this organ. Macroscopic examination of the pancreas of mute swans revealed hemorrhages as well as necrosis, while there were no macroscopic visible lesions in the pancreas of infected hens. Despite the different macroscopic finding, microscopic examination of the pancreas of both infected bird species revealed lesions in the form of acute pancreatitis and multifocal acinar necrosis. The viral antigen was abundantly expressed in the cytoplasm and nucleus of necrotic cells, as well as in macrophages in both examined bird species. Immunohistochemical expression of the viral antigen in the pancreas was strongly consistent with histological lesions. According to the above descnbed findings, it could be concluded that highly pathogenic avian influenza virus H5N8 has a high affinity to pancreatic tissue in both mute swans and hens and the distribution and the character of the lesions in the pancreas are similar in both bird species.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8",
volume = "68",
number = "2",
pages = "217-223",
doi = "10.2478/acve-2018-0018"
}

Bozić, B., Polaček, V., Vučićević, I., Vidanović, D., Vasković, N., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(2), 217-223.
https://doi.org/10.2478/acve-2018-0018

Bozić B, Polaček V, Vučićević I, Vidanović D, Vasković N, Prodanov-Radulović J, Aleksić-Kovačević S. Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8. in Acta Veterinaria-Beograd. 2018;68(2):217-223.
doi:10.2478/acve-2018-0018 .

Bozić, Biljana, Polaček, Vladimir, Vučićević, Ivana, Vidanović, Dejan, Vasković, Nikola, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8" in Acta Veterinaria-Beograd, 68, no. 2 (2018):217-223,
https://doi.org/10.2478/acve-2018-0018 . .

TY  - JOUR
AU  - Rašeta, Mladen
AU  - Mrdović, Boris
AU  - Đorđević, Vesna
AU  - Polaček, Vladimir
AU  - Becskei, Zsolt
AU  - Branković-Lazić, Ivana
AU  - Vasilev, Dragan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1609
AB  - Introduction. Heat treatment of food provides appropriate shelf life and safety, as well as adequate sensory properties and better digestibility, but excessive thermal processing leads to the loss of nutritive value. In practice, sterilised meat products are usually overheated, which leads to a loss of nutritive value. Therefore, it is necessary to find the optimum between the preservative effect of heat treatment and maintenance of the nutritive value of food. Control of the sterilisation efficiency is based on the Fo value, an indicator of the lethal effect of heat treatment, while the cooking (Co) value indicates a reduction of the nutritive value of heat treated products. Materials and Methods. During pâté sterilisation processes, two working heat treatment regimes were used: the regime used in regular, commercial production and an optimised regime. Heat treatment measurements were carried out using six thermocouple probes placed in the geothermal centres of six chosen cans. For each heat treatment, schematic diagrams were produced. Fo values were determined using Ellab software, and Co values by the graphic method in a semi-logarithmic Thermal Death Time diagram. Results and Conclusions. During pâté sterilisation, where the effective time was 55 minutes (regular regime) at 114 °C and 3.2 bar pressure, an average Fo value of 7.90±0.43 was achieved. By reducing the effective sterilisation time to 45 minutes (optimised regime), an average Fo value of 3.81±0.5 was achieved. Both regimes were sufficient to ensure product safety (Fo >3). The average Co value for the regular sterilisation regime was 109.83±1.33 minutes, while for the optimised regime, it was 88.67±4.27 minutes, i.e., an average reduction of 19.26%. This corresponds to a proportionally higher nutritional value of the product treated using the optimised regime. Data on the Fo and Co values achieved during the sterilisation process can make a significant contribution to obtaining products with a better nutritional value through an optimised sterilisation process, without jeopardising product safety.
AB  - Uvod. Toplotna obrda obzbeđuje održivost i bezbednost kao i adekvatna senzorna svojstva i bolju svarljivost hrane, ali suviše intenzivna topltona obrada dovodi do gubitka hranljive vrednosti. U redovnoj proizvodnji, sterilisani proizvodi od mesa se uobičajeno obrađuju suviše intenzivnim postupkom toplotne obrade, što dovodi do većeg dubitka hranljive vrednosti. Iz tog razloga, neophodno je da se pronađe optimum između konzervišućeg efekta toplotne obrade i očuvanja hranljive vrednosti hrane. Kontrola efkasnosti sterilizacije se bazira na određivanju Fo vrednsoti kao pokazatelja letalnog efekta toplotne obrade, dok Co vrednost (Cooking value) pokazuje smanjenje hranljive vrednosti toplotom obrađenih proizvoda. Materijal i metode. Za vreme sterilizacije paštete, primenjena su dva radna modela: postupak 'A' koji se koristi u redovnoj proizvodnji (uobičajeni režim) i postupak 'B' (optimizovani režim). Merenja su sprovedena pomoću 6 termopar sondi, koje su smeštene u geotermalnom centru 6 odabranih konzervi. Za svaki od toplotnih tretmana, iscrtani su dijagrami. Fo vrednost je određena Ellab softverom, a Co vrednost grafčkom metodom na polulogaritamskom TDT dijagramu. Rezultati i zaključak. Rezultati su pokazali da za vreme sterilizacije paštete, gde je efektivno vreme sterilizacije trajalo 55 min (uobičajeni režim), pri 114oC i pritisku od 3,2 bara, prosečna Fo vrednost iznosila 7,90 ± 0,43. Redukovanjem efektinog vremena sterilizacije na 45 minuta (optimizovani režim), postignuta je Fo vrednsot od 3,81 ± 0,5, pri čemu su oba režima bila dovoljna da osiguraju bezbednost proizvoda (Fo> 3). Co vrednost za uobičajeni režim sterilzacije je iznosila 109,83 ± 1,33 min, a za optimizovani režim 88.67 ± 4.27 min, pri čemu je ostvaren stepen redukcije od 19,26%, što odgovara proporcijonalno većoj hranljivoj vrednosti proizvoda tretiranog redukovanim režimom. Podaci o ostvarnim Fo i Co vrednostima za vreme postupka sterilizacije mogu da daju značajan doprinos u dobijanju proizvoda sa boljom hranljivom vrednosti kroz blaže postupke sterilizacije, a da se pri tome ne ugrozi bezbednost proizvoda.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Determination of Co-value as an indicator of nutritive value of pate sterilised by regular and optimized regime
T1  - Određivanje Co-vrednosti kao pokazatelja hranljive vrednosti paštete sterilisane uobičajenim i optimizovanim postupkom
VL  - 72
IS  - 2
SP  - 101
EP  - 111
DO  - 10.2298/VETGL180711011R
ER  - 

@article{
author = "Rašeta, Mladen and Mrdović, Boris and Đorđević, Vesna and Polaček, Vladimir and Becskei, Zsolt and Branković-Lazić, Ivana and Vasilev, Dragan",
year = "2018",
abstract = "Introduction. Heat treatment of food provides appropriate shelf life and safety, as well as adequate sensory properties and better digestibility, but excessive thermal processing leads to the loss of nutritive value. In practice, sterilised meat products are usually overheated, which leads to a loss of nutritive value. Therefore, it is necessary to find the optimum between the preservative effect of heat treatment and maintenance of the nutritive value of food. Control of the sterilisation efficiency is based on the Fo value, an indicator of the lethal effect of heat treatment, while the cooking (Co) value indicates a reduction of the nutritive value of heat treated products. Materials and Methods. During pâté sterilisation processes, two working heat treatment regimes were used: the regime used in regular, commercial production and an optimised regime. Heat treatment measurements were carried out using six thermocouple probes placed in the geothermal centres of six chosen cans. For each heat treatment, schematic diagrams were produced. Fo values were determined using Ellab software, and Co values by the graphic method in a semi-logarithmic Thermal Death Time diagram. Results and Conclusions. During pâté sterilisation, where the effective time was 55 minutes (regular regime) at 114 °C and 3.2 bar pressure, an average Fo value of 7.90±0.43 was achieved. By reducing the effective sterilisation time to 45 minutes (optimised regime), an average Fo value of 3.81±0.5 was achieved. Both regimes were sufficient to ensure product safety (Fo >3). The average Co value for the regular sterilisation regime was 109.83±1.33 minutes, while for the optimised regime, it was 88.67±4.27 minutes, i.e., an average reduction of 19.26%. This corresponds to a proportionally higher nutritional value of the product treated using the optimised regime. Data on the Fo and Co values achieved during the sterilisation process can make a significant contribution to obtaining products with a better nutritional value through an optimised sterilisation process, without jeopardising product safety., Uvod. Toplotna obrda obzbeđuje održivost i bezbednost kao i adekvatna senzorna svojstva i bolju svarljivost hrane, ali suviše intenzivna topltona obrada dovodi do gubitka hranljive vrednosti. U redovnoj proizvodnji, sterilisani proizvodi od mesa se uobičajeno obrađuju suviše intenzivnim postupkom toplotne obrade, što dovodi do većeg dubitka hranljive vrednosti. Iz tog razloga, neophodno je da se pronađe optimum između konzervišućeg efekta toplotne obrade i očuvanja hranljive vrednosti hrane. Kontrola efkasnosti sterilizacije se bazira na određivanju Fo vrednsoti kao pokazatelja letalnog efekta toplotne obrade, dok Co vrednost (Cooking value) pokazuje smanjenje hranljive vrednosti toplotom obrađenih proizvoda. Materijal i metode. Za vreme sterilizacije paštete, primenjena su dva radna modela: postupak 'A' koji se koristi u redovnoj proizvodnji (uobičajeni režim) i postupak 'B' (optimizovani režim). Merenja su sprovedena pomoću 6 termopar sondi, koje su smeštene u geotermalnom centru 6 odabranih konzervi. Za svaki od toplotnih tretmana, iscrtani su dijagrami. Fo vrednost je određena Ellab softverom, a Co vrednost grafčkom metodom na polulogaritamskom TDT dijagramu. Rezultati i zaključak. Rezultati su pokazali da za vreme sterilizacije paštete, gde je efektivno vreme sterilizacije trajalo 55 min (uobičajeni režim), pri 114oC i pritisku od 3,2 bara, prosečna Fo vrednost iznosila 7,90 ± 0,43. Redukovanjem efektinog vremena sterilizacije na 45 minuta (optimizovani režim), postignuta je Fo vrednsot od 3,81 ± 0,5, pri čemu su oba režima bila dovoljna da osiguraju bezbednost proizvoda (Fo> 3). Co vrednost za uobičajeni režim sterilzacije je iznosila 109,83 ± 1,33 min, a za optimizovani režim 88.67 ± 4.27 min, pri čemu je ostvaren stepen redukcije od 19,26%, što odgovara proporcijonalno većoj hranljivoj vrednosti proizvoda tretiranog redukovanim režimom. Podaci o ostvarnim Fo i Co vrednostima za vreme postupka sterilizacije mogu da daju značajan doprinos u dobijanju proizvoda sa boljom hranljivom vrednosti kroz blaže postupke sterilizacije, a da se pri tome ne ugrozi bezbednost proizvoda.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Determination of Co-value as an indicator of nutritive value of pate sterilised by regular and optimized regime, Određivanje Co-vrednosti kao pokazatelja hranljive vrednosti paštete sterilisane uobičajenim i optimizovanim postupkom",
volume = "72",
number = "2",
pages = "101-111",
doi = "10.2298/VETGL180711011R"
}

Rašeta, M., Mrdović, B., Đorđević, V., Polaček, V., Becskei, Z., Branković-Lazić, I.,& Vasilev, D.. (2018). Determination of Co-value as an indicator of nutritive value of pate sterilised by regular and optimized regime. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 101-111.
https://doi.org/10.2298/VETGL180711011R

Rašeta M, Mrdović B, Đorđević V, Polaček V, Becskei Z, Branković-Lazić I, Vasilev D. Determination of Co-value as an indicator of nutritive value of pate sterilised by regular and optimized regime. in Veterinarski Glasnik. 2018;72(2):101-111.
doi:10.2298/VETGL180711011R .

Rašeta, Mladen, Mrdović, Boris, Đorđević, Vesna, Polaček, Vladimir, Becskei, Zsolt, Branković-Lazić, Ivana, Vasilev, Dragan, "Determination of Co-value as an indicator of nutritive value of pate sterilised by regular and optimized regime" in Veterinarski Glasnik, 72, no. 2 (2018):101-111,
https://doi.org/10.2298/VETGL180711011R . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Božić, Biljana
AU  - Prodanov-Radulović, Jasna
AU  - Tamaš, Petrović
AU  - Vučićević, Ivana
AU  - Becskei, Zsolt
AU  - Aleksić-Kovačević, Sanja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2823
PB  - Elsevier
T2  - Journal of Comparative Pathology
T1  - Immunohistochemical detection of classical swine fewer virus in fetuses from naturally infected sows
VL  - 156
IS  - 1
SP  - 102
EP  - 102
DO  - 10.1016/j.jcpa.2016.11.154
ER  - 

@article{
author = "Polaček, Vladimir and Božić, Biljana and Prodanov-Radulović, Jasna and Tamaš, Petrović and Vučićević, Ivana and Becskei, Zsolt and Aleksić-Kovačević, Sanja",
year = "2017",
publisher = "Elsevier",
journal = "Journal of Comparative Pathology",
title = "Immunohistochemical detection of classical swine fewer virus in fetuses from naturally infected sows",
volume = "156",
number = "1",
pages = "102-102",
doi = "10.1016/j.jcpa.2016.11.154"
}

Polaček, V., Božić, B., Prodanov-Radulović, J., Tamaš, P., Vučićević, I., Becskei, Z.,& Aleksić-Kovačević, S.. (2017). Immunohistochemical detection of classical swine fewer virus in fetuses from naturally infected sows. in Journal of Comparative Pathology
Elsevier., 156(1), 102-102.
https://doi.org/10.1016/j.jcpa.2016.11.154

Polaček V, Božić B, Prodanov-Radulović J, Tamaš P, Vučićević I, Becskei Z, Aleksić-Kovačević S. Immunohistochemical detection of classical swine fewer virus in fetuses from naturally infected sows. in Journal of Comparative Pathology. 2017;156(1):102-102.
doi:10.1016/j.jcpa.2016.11.154 .

Polaček, Vladimir, Božić, Biljana, Prodanov-Radulović, Jasna, Tamaš, Petrović, Vučićević, Ivana, Becskei, Zsolt, Aleksić-Kovačević, Sanja, "Immunohistochemical detection of classical swine fewer virus in fetuses from naturally infected sows" in Journal of Comparative Pathology, 156, no. 1 (2017):102-102,
https://doi.org/10.1016/j.jcpa.2016.11.154 . .

TY  - CONF
AU  - Rašeta, Mladen
AU  - Mrdović, Boris
AU  - Janković, V.
AU  - Becskei, Zsolt
AU  - Lakićević, Brankica
AU  - Vidanović, Dejan
AU  - Polaček, Vladimir
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1551
AB  - This study aimed to determine Salmonella spp. prevalence in meat products, meat preparations and minced meat. Over a period of three years, a total of 300 samples were taken (100 RTE meat products, 100 meat preparations and 100 minced meat) and examined for the presence of Salmonella spp. Sampling was carried out at the warehouses of the food manufacturers. Salmonella spp. were not detected in RTE meat products, while 7% of semi-finished meat products (fresh sausages, grill meat formed and unformed) contained Salmonella, as did 18% of minced meats (minced pork II category, minced beef II category, mixed minced meat). The 25 Salmonella isolates obtained were examined for antibiotic resistance by the disk diffusion test, according to the NCCLS and CLSI guidelines. Isolates showed resistance to ampicillin and nalidixic acid (80%), tetracycline (72%), cefotaxime/clavulanic acid (48%), but not to gentamicin (8%) or trimethoprim/sulfamethoxazole (0%).
PB  - Iop Publishing Ltd, Bristol
C3  - 59th International Meat Industry Conference (MEATCON2017)
T1  - Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat
VL  - 85
SP  - UNSP 012028
DO  - 10.1088/1755-1315/85/1/012028
ER  - 

@conference{
author = "Rašeta, Mladen and Mrdović, Boris and Janković, V. and Becskei, Zsolt and Lakićević, Brankica and Vidanović, Dejan and Polaček, Vladimir",
year = "2017",
abstract = "This study aimed to determine Salmonella spp. prevalence in meat products, meat preparations and minced meat. Over a period of three years, a total of 300 samples were taken (100 RTE meat products, 100 meat preparations and 100 minced meat) and examined for the presence of Salmonella spp. Sampling was carried out at the warehouses of the food manufacturers. Salmonella spp. were not detected in RTE meat products, while 7% of semi-finished meat products (fresh sausages, grill meat formed and unformed) contained Salmonella, as did 18% of minced meats (minced pork II category, minced beef II category, mixed minced meat). The 25 Salmonella isolates obtained were examined for antibiotic resistance by the disk diffusion test, according to the NCCLS and CLSI guidelines. Isolates showed resistance to ampicillin and nalidixic acid (80%), tetracycline (72%), cefotaxime/clavulanic acid (48%), but not to gentamicin (8%) or trimethoprim/sulfamethoxazole (0%).",
publisher = "Iop Publishing Ltd, Bristol",
journal = "59th International Meat Industry Conference (MEATCON2017)",
title = "Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat",
volume = "85",
pages = "UNSP 012028",
doi = "10.1088/1755-1315/85/1/012028"
}

Rašeta, M., Mrdović, B., Janković, V., Becskei, Z., Lakićević, B., Vidanović, D.,& Polaček, V.. (2017). Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat. in 59th International Meat Industry Conference (MEATCON2017)
Iop Publishing Ltd, Bristol., 85, UNSP 012028.
https://doi.org/10.1088/1755-1315/85/1/012028

Rašeta M, Mrdović B, Janković V, Becskei Z, Lakićević B, Vidanović D, Polaček V. Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat. in 59th International Meat Industry Conference (MEATCON2017). 2017;85:UNSP 012028.
doi:10.1088/1755-1315/85/1/012028 .

Rašeta, Mladen, Mrdović, Boris, Janković, V., Becskei, Zsolt, Lakićević, Brankica, Vidanović, Dejan, Polaček, Vladimir, "Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat" in 59th International Meat Industry Conference (MEATCON2017), 85 (2017):UNSP 012028,
https://doi.org/10.1088/1755-1315/85/1/012028 . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Becskei, Zsolt
AU  - Petrović, Tamaš
AU  - Polaček, Vladimir
AU  - Ristić, Bojan
AU  - Milić, Sinisa
AU  - Stepanović, Predrag
AU  - Radisavljević, Katarina
AU  - Dimitrijević, Sanda
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1395
AB  - Wild canides have a high epizootiological - epidemiological significance, considering that they are hosts for some parasites which spread vector born diseases. Increased frequency of certain interactions between domestic and wild canides increases the risk of occurrence, spreading and maintaining the infection of parasitic etiology in domestic canides. The research was conducted in 232 wild canides (172 red foxes and 60 golden jackals). The examined material was sampled from foxes and jackals, which were hunted down between 2010 and 2014, from 8 epizootiological areas of Serbia (North-Backa, West-Backa, Southern-Banat, Moravicki, Zlatiborski, Raski, Rasinski and Zajecarski district). On completing the parasitological dissection and the coprological diagnostics, in wild canides protozoa from the genus Isospora were identified, 3 species of trematoda (Alaria alata, Pseudamphistomum truncatum and Metagonimus yokogawai), cestods from the genus Taenia and 5 species of nematodes (Toxocara canis, Ancylostomatidae, Trichuris vulpis and Capillaria aerophila). The finding of M. yokogawai in golden jackals were, to the best of our knowledge, one of the first diagnosed cases of metagonimosis in golden jackals in Serbia. The continued monitoring of the parasitic fauna of wild canides is needed to establish the widespread of the zoonoses in different regions of Serbia, because they present the reservoirs and/or sources of these infections.
PB  - Springer International Publishing Ag, Cham
T2  - Acta Parasitologica
T1  - Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia
VL  - 61
IS  - 2
SP  - 389
EP  - 396
DO  - 10.1515/ap-2016-0051
ER  - 

@article{
author = "Ilić, Tamara and Becskei, Zsolt and Petrović, Tamaš and Polaček, Vladimir and Ristić, Bojan and Milić, Sinisa and Stepanović, Predrag and Radisavljević, Katarina and Dimitrijević, Sanda",
year = "2016",
abstract = "Wild canides have a high epizootiological - epidemiological significance, considering that they are hosts for some parasites which spread vector born diseases. Increased frequency of certain interactions between domestic and wild canides increases the risk of occurrence, spreading and maintaining the infection of parasitic etiology in domestic canides. The research was conducted in 232 wild canides (172 red foxes and 60 golden jackals). The examined material was sampled from foxes and jackals, which were hunted down between 2010 and 2014, from 8 epizootiological areas of Serbia (North-Backa, West-Backa, Southern-Banat, Moravicki, Zlatiborski, Raski, Rasinski and Zajecarski district). On completing the parasitological dissection and the coprological diagnostics, in wild canides protozoa from the genus Isospora were identified, 3 species of trematoda (Alaria alata, Pseudamphistomum truncatum and Metagonimus yokogawai), cestods from the genus Taenia and 5 species of nematodes (Toxocara canis, Ancylostomatidae, Trichuris vulpis and Capillaria aerophila). The finding of M. yokogawai in golden jackals were, to the best of our knowledge, one of the first diagnosed cases of metagonimosis in golden jackals in Serbia. The continued monitoring of the parasitic fauna of wild canides is needed to establish the widespread of the zoonoses in different regions of Serbia, because they present the reservoirs and/or sources of these infections.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Acta Parasitologica",
title = "Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia",
volume = "61",
number = "2",
pages = "389-396",
doi = "10.1515/ap-2016-0051"
}

Ilić, T., Becskei, Z., Petrović, T., Polaček, V., Ristić, B., Milić, S., Stepanović, P., Radisavljević, K.,& Dimitrijević, S.. (2016). Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia. in Acta Parasitologica
Springer International Publishing Ag, Cham., 61(2), 389-396.
https://doi.org/10.1515/ap-2016-0051

Ilić T, Becskei Z, Petrović T, Polaček V, Ristić B, Milić S, Stepanović P, Radisavljević K, Dimitrijević S. Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia. in Acta Parasitologica. 2016;61(2):389-396.
doi:10.1515/ap-2016-0051 .

Ilić, Tamara, Becskei, Zsolt, Petrović, Tamaš, Polaček, Vladimir, Ristić, Bojan, Milić, Sinisa, Stepanović, Predrag, Radisavljević, Katarina, Dimitrijević, Sanda, "Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia" in Acta Parasitologica, 61, no. 2 (2016):389-396,
https://doi.org/10.1515/ap-2016-0051 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1356
AB  - Although Mycobacterium avium subspecies are generally not considered food pathogens, the infections caused by these particular nontuberculous mycobacteria (NTM) can represent a serious threat to immunocompromised population. Additionally, infections with a member of Mycobacterium Avium Compex (MAC) can affect the efficiency of BCG vaccines used for the humans. In infected animals, M. avium may be present in different tissues without apparent clinical symptoms and macroscopic lesions. Veterinary meat inspection would then fail to recognize infected animals and such meat and meat products thereof could enter the human diet. The aim of this paper is also to analyze the current control policy in Europe according to infections of pigs with the members of MAC, and point out the risks for public health. By analyzing a large number of meat samples and other dietary nutrients, different groups of authors have provided evidence to support the hypothesis that M. avium is present in the everyday environment. Therefore, food as a source of infection with mycobacteria should not be ignored. The control of mycobacteria requires a better diagnostic approach, having in mind recent positive cases of M. avium subspecies hominissuis (MAH) in an increasing number of exported pigs from EU countries to Serbia. The introduction of reliable diagnostic methods for MAH could result in decreasing the occurrence of infection in pigs, as well as in humans, having in mind that WHO reported 10 million new cases of tuberculosis-mycobacteriosis in the human population in 2015 with 21% of these cases occurring in immunocompromised individuals and children.
AB  - Mada se pripadnici podvrste Mycobacterium avium ne smatraju uzročnicima koji se primarno prenose putem hrane, infekcija ovim netuberkuloznim mikobakterijama (NTM) može predstavljati značajnu pretnju za zdravlje imunokompromitovanih jedinki. Infekcija pripadnicima Mycobacterium avium kompleksa (MAC) može da utiče na BCG imunitet u humanoj populaciji. U inficiranim životinjama M. avium može biti prisutan u različitim organima bez izraženih kliničkih simptoma i morfoloških manifestacija. Iz tog razloga veterinarska inspekcija na liniji klanja nema mogućnost da detektuje inficirane životinje, pa meso i mesni proizvodi od ovih životinja mogu ući u lanac ljudske ishrane. Cilj ovog rada je da iznese i trenutnu politiku kontrole infekcije svinja pripadnicima MAC-a u Evropi, kao i da ukaže na glavne potencijalne rizike. Analizom velikog broja uzoraka mesa i drugih namernica za ljudsku ishranu, različite grupe autora u svojim istraživanjima dokazale su prisustvo M. avium, pa se hrana kao izvor infekcije mikobakterijama ipak ne sme zanemariti. Kontrola mikobakterija zahteva bolji dijagnostički pristup, uzimajući u obzir i povećan broj slučajeva infekcija M. avium subsp hominissuis kod svinja koje su se uvozile u Srbiju iz zemalja EU proteklih godina. Uvođenje pouzdanih dijagnostičkih metoda za utvrđivanje MAH uzročnika trebalo bi da smanjeni rizik od infekcije kod svinja, pa i kod ljudi, imajući u vidu da je na osnovu podataka Svetske zdravstvene organizacije registrovano 10 miliona novih slučajeva tuberkuloze odn. mikobakterioze ljudi u toku 2015. godine, a 21% ovih slučajeva zabeleženo je upravo u populaciji imunokopromitovanih jedinki.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Mycobacteriosis in pigs: An underrated threat
T1  - Mikobakterioza svinja - potcenjena pretnja
VL  - 66
IS  - 4
SP  - 429
EP  - 443
DO  - 10.1515/acve-2016-0037
ER  - 

@article{
author = "Polaček, Vladimir and Aleksić-Kovačević, Sanja",
year = "2016",
abstract = "Although Mycobacterium avium subspecies are generally not considered food pathogens, the infections caused by these particular nontuberculous mycobacteria (NTM) can represent a serious threat to immunocompromised population. Additionally, infections with a member of Mycobacterium Avium Compex (MAC) can affect the efficiency of BCG vaccines used for the humans. In infected animals, M. avium may be present in different tissues without apparent clinical symptoms and macroscopic lesions. Veterinary meat inspection would then fail to recognize infected animals and such meat and meat products thereof could enter the human diet. The aim of this paper is also to analyze the current control policy in Europe according to infections of pigs with the members of MAC, and point out the risks for public health. By analyzing a large number of meat samples and other dietary nutrients, different groups of authors have provided evidence to support the hypothesis that M. avium is present in the everyday environment. Therefore, food as a source of infection with mycobacteria should not be ignored. The control of mycobacteria requires a better diagnostic approach, having in mind recent positive cases of M. avium subspecies hominissuis (MAH) in an increasing number of exported pigs from EU countries to Serbia. The introduction of reliable diagnostic methods for MAH could result in decreasing the occurrence of infection in pigs, as well as in humans, having in mind that WHO reported 10 million new cases of tuberculosis-mycobacteriosis in the human population in 2015 with 21% of these cases occurring in immunocompromised individuals and children., Mada se pripadnici podvrste Mycobacterium avium ne smatraju uzročnicima koji se primarno prenose putem hrane, infekcija ovim netuberkuloznim mikobakterijama (NTM) može predstavljati značajnu pretnju za zdravlje imunokompromitovanih jedinki. Infekcija pripadnicima Mycobacterium avium kompleksa (MAC) može da utiče na BCG imunitet u humanoj populaciji. U inficiranim životinjama M. avium može biti prisutan u različitim organima bez izraženih kliničkih simptoma i morfoloških manifestacija. Iz tog razloga veterinarska inspekcija na liniji klanja nema mogućnost da detektuje inficirane životinje, pa meso i mesni proizvodi od ovih životinja mogu ući u lanac ljudske ishrane. Cilj ovog rada je da iznese i trenutnu politiku kontrole infekcije svinja pripadnicima MAC-a u Evropi, kao i da ukaže na glavne potencijalne rizike. Analizom velikog broja uzoraka mesa i drugih namernica za ljudsku ishranu, različite grupe autora u svojim istraživanjima dokazale su prisustvo M. avium, pa se hrana kao izvor infekcije mikobakterijama ipak ne sme zanemariti. Kontrola mikobakterija zahteva bolji dijagnostički pristup, uzimajući u obzir i povećan broj slučajeva infekcija M. avium subsp hominissuis kod svinja koje su se uvozile u Srbiju iz zemalja EU proteklih godina. Uvođenje pouzdanih dijagnostičkih metoda za utvrđivanje MAH uzročnika trebalo bi da smanjeni rizik od infekcije kod svinja, pa i kod ljudi, imajući u vidu da je na osnovu podataka Svetske zdravstvene organizacije registrovano 10 miliona novih slučajeva tuberkuloze odn. mikobakterioze ljudi u toku 2015. godine, a 21% ovih slučajeva zabeleženo je upravo u populaciji imunokopromitovanih jedinki.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Mycobacteriosis in pigs: An underrated threat, Mikobakterioza svinja - potcenjena pretnja",
volume = "66",
number = "4",
pages = "429-443",
doi = "10.1515/acve-2016-0037"
}

Polaček, V.,& Aleksić-Kovačević, S.. (2016). Mycobacteriosis in pigs: An underrated threat. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 429-443.
https://doi.org/10.1515/acve-2016-0037

Polaček V, Aleksić-Kovačević S. Mycobacteriosis in pigs: An underrated threat. in Acta Veterinaria-Beograd. 2016;66(4):429-443.
doi:10.1515/acve-2016-0037 .

Polaček, Vladimir, Aleksić-Kovačević, Sanja, "Mycobacteriosis in pigs: An underrated threat" in Acta Veterinaria-Beograd, 66, no. 4 (2016):429-443,
https://doi.org/10.1515/acve-2016-0037 . .

TY  - JOUR
AU  - Pelić, Miloš
AU  - Božić, Biljana
AU  - Pajić, Marko
AU  - Vučićević, Ivana
AU  - Samojlović, Milena
AU  - Polaček, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1327
AB  - The two corpses of police dogs, of Labrador retriever breed used for fi nding narcotics, were sent to the Scientific Veterinary Institute 'Novi Sad', and examined post-mortem, in order to determine the cause of death. Post-mortem examination revealed the presence of neoplastic changes in the mandibular region, pulmonary parenchyma and axillary region. The samples of neoplastic changes were sent to the Laboratory of Patohistology within the Faculty of Veterinary Medicine, University of Belgrade, in order to specify the diagnosis. Histopathology examination revealed an adenocarcinoma in lungs and haemangioendothelioma in axillary region. Is there any role of drug sniffing on the occurrence of neoplasia in Labrador retriever breed is still unknown, but it could be an important factor in the development of neoplasia in these dogs.
AB  - Dva leša policijskih službenih pasa, rase Labrador retriver koji se koriste za pronalaženje narkotika, dostavljeni su u Naučni institut za veterinarstvo 'Novi Sad', kako bi se na osnovu patomorfološkog pregleda utvrdio uzrok smrti. Tokom obdukcije utvrđeno je prisustvo neoplastičnih promena u mandibularnoj regiji, plućnom parenhimu i aksilarnoj regiji. Uzorci promenjenog tkiva dostavljeni su na Fakultet veterinarske medicine, Univerziteta u Beogradu, u laboratoriju za patohistologiju kako bi se odredila tačna dijagnoza. Patohistološkim nalazom utvrđeno je prisustvo adenokarcinoma u plućima i hemangioendotelioma u predelu aksilarne regije. Kakvu ulogu može imati udisanje narkotika na pojavu neoplazmi kod Labrador retrivera još uvek nije poznato, ali se pretpostavlja da to može biti jedan od faktora u nastanku neoplastičnih promena kod ovih službenih pasa.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Malignant tumours in Labrador retrievers used for narcotic detection
T1  - Maligni tumori kod Labrador retrivera koji se koriste za pronalaženje narkotika
VL  - 9
IS  - 1
SP  - 93
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1327
ER  - 

@article{
author = "Pelić, Miloš and Božić, Biljana and Pajić, Marko and Vučićević, Ivana and Samojlović, Milena and Polaček, Vladimir",
year = "2016",
abstract = "The two corpses of police dogs, of Labrador retriever breed used for fi nding narcotics, were sent to the Scientific Veterinary Institute 'Novi Sad', and examined post-mortem, in order to determine the cause of death. Post-mortem examination revealed the presence of neoplastic changes in the mandibular region, pulmonary parenchyma and axillary region. The samples of neoplastic changes were sent to the Laboratory of Patohistology within the Faculty of Veterinary Medicine, University of Belgrade, in order to specify the diagnosis. Histopathology examination revealed an adenocarcinoma in lungs and haemangioendothelioma in axillary region. Is there any role of drug sniffing on the occurrence of neoplasia in Labrador retriever breed is still unknown, but it could be an important factor in the development of neoplasia in these dogs., Dva leša policijskih službenih pasa, rase Labrador retriver koji se koriste za pronalaženje narkotika, dostavljeni su u Naučni institut za veterinarstvo 'Novi Sad', kako bi se na osnovu patomorfološkog pregleda utvrdio uzrok smrti. Tokom obdukcije utvrđeno je prisustvo neoplastičnih promena u mandibularnoj regiji, plućnom parenhimu i aksilarnoj regiji. Uzorci promenjenog tkiva dostavljeni su na Fakultet veterinarske medicine, Univerziteta u Beogradu, u laboratoriju za patohistologiju kako bi se odredila tačna dijagnoza. Patohistološkim nalazom utvrđeno je prisustvo adenokarcinoma u plućima i hemangioendotelioma u predelu aksilarne regije. Kakvu ulogu može imati udisanje narkotika na pojavu neoplazmi kod Labrador retrivera još uvek nije poznato, ali se pretpostavlja da to može biti jedan od faktora u nastanku neoplastičnih promena kod ovih službenih pasa.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Malignant tumours in Labrador retrievers used for narcotic detection, Maligni tumori kod Labrador retrivera koji se koriste za pronalaženje narkotika",
volume = "9",
number = "1",
pages = "93-101",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1327"
}

Pelić, M., Božić, B., Pajić, M., Vučićević, I., Samojlović, M.,& Polaček, V.. (2016). Malignant tumours in Labrador retrievers used for narcotic detection. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(1), 93-101.
https://hdl.handle.net/21.15107/rcub_veterinar_1327

Pelić M, Božić B, Pajić M, Vučićević I, Samojlović M, Polaček V. Malignant tumours in Labrador retrievers used for narcotic detection. in Arhiv veterinarske medicine. 2016;9(1):93-101.
https://hdl.handle.net/21.15107/rcub_veterinar_1327 .

Pelić, Miloš, Božić, Biljana, Pajić, Marko, Vučićević, Ivana, Samojlović, Milena, Polaček, Vladimir, "Malignant tumours in Labrador retrievers used for narcotic detection" in Arhiv veterinarske medicine, 9, no. 1 (2016):93-101,
https://hdl.handle.net/21.15107/rcub_veterinar_1327 .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Polaček, Vladimir
AU  - Suvajdzić, Ljiljana
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1414
AB  - Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Journal of Veterinary Research
T1  - Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms
VL  - 60
IS  - 4
SP  - 373
EP  - 378
DO  - 10.1515/jvetres-2016-0054
ER  - 

@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Polaček, Vladimir and Suvajdzić, Ljiljana and Bojkovski, Jovan",
year = "2016",
abstract = "Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Journal of Veterinary Research",
title = "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms",
volume = "60",
number = "4",
pages = "373-378",
doi = "10.1515/jvetres-2016-0054"
}

Milanov, D., Petrović, T., Polaček, V., Suvajdzić, L.,& Bojkovski, J.. (2016). Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research
Walter De Gruyter Gmbh, Berlin., 60(4), 373-378.
https://doi.org/10.1515/jvetres-2016-0054

Milanov D, Petrović T, Polaček V, Suvajdzić L, Bojkovski J. Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research. 2016;60(4):373-378.
doi:10.1515/jvetres-2016-0054 .

Milanov, Dubravka, Petrović, Tamaš, Polaček, Vladimir, Suvajdzić, Ljiljana, Bojkovski, Jovan, "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms" in Journal of Veterinary Research, 60, no. 4 (2016):373-378,
https://doi.org/10.1515/jvetres-2016-0054 . .

TY  - JOUR
AU  - Becskei, Zsolt
AU  - Novosel, Dinko
AU  - Polaček, Vladimir
AU  - Cadar, Daniel
AU  - Gavrilović, Pavle
AU  - Savić, Mila
AU  - Aleksić-Kovačević, Sanja
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2824
PB  - Elsevier
T2  - Journal of Comparative Pathology
T1  - Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus associated disease (PCVAD) of wild boars in  Serbia
VL  - 152
IS  - 1
SP  - 86
EP  - 86
DO  - 10.1016/j.jcpa.2014.10.181
ER  - 

@article{
author = "Becskei, Zsolt and Novosel, Dinko and Polaček, Vladimir and Cadar, Daniel and Gavrilović, Pavle and Savić, Mila and Aleksić-Kovačević, Sanja",
year = "2015",
publisher = "Elsevier",
journal = "Journal of Comparative Pathology",
title = "Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus associated disease (PCVAD) of wild boars in  Serbia",
volume = "152",
number = "1",
pages = "86-86",
doi = "10.1016/j.jcpa.2014.10.181"
}

Becskei, Z., Novosel, D., Polaček, V., Cadar, D., Gavrilović, P., Savić, M.,& Aleksić-Kovačević, S.. (2015). Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus associated disease (PCVAD) of wild boars in  Serbia. in Journal of Comparative Pathology
Elsevier., 152(1), 86-86.
https://doi.org/10.1016/j.jcpa.2014.10.181

Becskei Z, Novosel D, Polaček V, Cadar D, Gavrilović P, Savić M, Aleksić-Kovačević S. Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus associated disease (PCVAD) of wild boars in  Serbia. in Journal of Comparative Pathology. 2015;152(1):86-86.
doi:10.1016/j.jcpa.2014.10.181 .

Becskei, Zsolt, Novosel, Dinko, Polaček, Vladimir, Cadar, Daniel, Gavrilović, Pavle, Savić, Mila, Aleksić-Kovačević, Sanja, "Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus associated disease (PCVAD) of wild boars in  Serbia" in Journal of Comparative Pathology, 152, no. 1 (2015):86-86,
https://doi.org/10.1016/j.jcpa.2014.10.181 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Becskei, Zsolt
AU  - Prodanov-Radulović, Jasna
AU  - Aleksić-Kovačević, Sanja
AU  - Vidanović, D.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2825
PB  - Elsevier
T2  - Journal of Comparative Pathology
T1  - Detection of Mycobacterium avium subsp. hominissuis in lymph nodes with and without gross lesions from tuberculin skin test positive pigs
VL  - 152
IS  - 1
SP  - 83
EP  - 83
DO  - 10.1016/j.jcpa.2014.10.170
ER  - 

@article{
author = "Polaček, Vladimir and Becskei, Zsolt and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja and Vidanović, D.",
year = "2015",
publisher = "Elsevier",
journal = "Journal of Comparative Pathology",
title = "Detection of Mycobacterium avium subsp. hominissuis in lymph nodes with and without gross lesions from tuberculin skin test positive pigs",
volume = "152",
number = "1",
pages = "83-83",
doi = "10.1016/j.jcpa.2014.10.170"
}

Polaček, V., Becskei, Z., Prodanov-Radulović, J., Aleksić-Kovačević, S.,& Vidanović, D.. (2015). Detection of Mycobacterium avium subsp. hominissuis in lymph nodes with and without gross lesions from tuberculin skin test positive pigs. in Journal of Comparative Pathology
Elsevier., 152(1), 83-83.
https://doi.org/10.1016/j.jcpa.2014.10.170

Polaček V, Becskei Z, Prodanov-Radulović J, Aleksić-Kovačević S, Vidanović D. Detection of Mycobacterium avium subsp. hominissuis in lymph nodes with and without gross lesions from tuberculin skin test positive pigs. in Journal of Comparative Pathology. 2015;152(1):83-83.
doi:10.1016/j.jcpa.2014.10.170 .

Polaček, Vladimir, Becskei, Zsolt, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, Vidanović, D., "Detection of Mycobacterium avium subsp. hominissuis in lymph nodes with and without gross lesions from tuberculin skin test positive pigs" in Journal of Comparative Pathology, 152, no. 1 (2015):83-83,
https://doi.org/10.1016/j.jcpa.2014.10.170 . .

TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Polaček, Vladimir
AU  - Đurić, Spomenka
AU  - Stojanov, Igor
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1238
AB  - Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. .
AB  - Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region
T1  - Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu
VL  - 8
IS  - 1
SP  - 67
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1238
ER  - 

@article{
author = "Pajić, Marko and Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Polaček, Vladimir and Đurić, Spomenka and Stojanov, Igor",
year = "2015",
abstract = "Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. ., Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region, Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu",
volume = "8",
number = "1",
pages = "67-76",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1238"
}

Pajić, M., Todorović, D., Velhner, M., Milanov, D., Polaček, V., Đurić, S.,& Stojanov, I.. (2015). The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 8(1), 67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238

Pajić M, Todorović D, Velhner M, Milanov D, Polaček V, Đurić S, Stojanov I. The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine. 2015;8(1):67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .

Pajić, Marko, Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Polaček, Vladimir, Đurić, Spomenka, Stojanov, Igor, "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region" in Arhiv veterinarske medicine, 8, no. 1 (2015):67-76,
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .

TY  - JOUR
AU  - Rašeta, Mladen
AU  - Branković-Lazić, Ivana
AU  - Lukić, Mirjana
AU  - Teodorović, Vlado
AU  - Lakićević, Brankica
AU  - Polaček, Vladimir
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1246
AB  - The impact of disease on poultry production is one of the major limiting factors of successful performance in the poultry industry. Biosecurity can be defined as a way to assure safety from the transmission of infectious diseases, parasites and verbs. At the primary production level (broiler breeding) on a farm, the main problem is inconsistent implementation of biosecurity measures, as defined in the biosecurity protocol. Adequate implementation of biosecurity measures on farm depends from the least paid and educated workers and is subject to errors during everyday working practice. Therefore daily monitoring of employees' performance is necessary, with continual education, whereby each detected non-compliance serves to improve the existing biosecurity protocol. Since the prevalence of Salmonella spp. on broiler meat at the slaughterhouse, among other factors, depends on Salmonella spp. prevalence on farm, in cases where technological operations are standardized at the slaughterhouse and when the hygiene level is equable, the data on Salmonella spp. prevalence can help us indirectly to assess adequate implementation of biosecurity measures on farm. In our research, after strict and consistent implementation of biosecurity measures on farm, Salmonella spp. prevalence at the slaughterhouse was reduced from 24% to 10%.
AB  - Glavni ograničavajući faktor uspešnosti proizvodnje mesa brojlera je negativni uticaj potencijalnih bolesti. Biosigurnosne mere predstavljaju način osiguranja bezbednosti od prenosa infektivnih bolesti, parazita i ostalih uzročnika oboljenja. Na nivou primarne proizvodnje (tov brojlera) na farmi, problem predstavlja nedosledna primena biosigurnosnih mera koje su definisane u biosigurnosnom protokolu. Adekvatna primena biosigurnosnih mera na farmi zavisi od najmanje plaćenih i edukovanih radnika i podložna je greškama tokom obavljanja svakodnevne radne prakse. Stoga je potrebno svakodnevno praćenje izvršenja radnih obaveza zaposlenih, uz kontinuiranu edukaciju, pri čemu svaka otkrivena neusaglašenost služi za unapređenje postojećeg biosigurnosnog protokola. Prevalencija Salmonella spp., na mesu brojlera u klanici, između ostalih faktora zavisi i od nalaza Salmonella spp. na farmi. U slučaju kada su tehnološke operacije na liniji klanja standardizovane i postupak sprovođenja higijene ujednačen, podatak o prisustvu Salmonella spp., može poslužiti za procenu da li su primenjene adekvatne biosigurnosne mere na farmi. U našem radu, nakon striktnog i doslednog preduzimanja biosigurnosnih mera na farmi, prevalencija Salmonella spp. na koži vrata brojlera na klanici, smanjena je sa 24% na 10%.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Significance of biosecurity measures in primary broiler production
T1  - Značaj biosigurnosnih mera u primarnoj proizvodnji mesa brojlera
VL  - 56
IS  - 2
SP  - 91
EP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1246
ER  - 

@article{
author = "Rašeta, Mladen and Branković-Lazić, Ivana and Lukić, Mirjana and Teodorović, Vlado and Lakićević, Brankica and Polaček, Vladimir",
year = "2015",
abstract = "The impact of disease on poultry production is one of the major limiting factors of successful performance in the poultry industry. Biosecurity can be defined as a way to assure safety from the transmission of infectious diseases, parasites and verbs. At the primary production level (broiler breeding) on a farm, the main problem is inconsistent implementation of biosecurity measures, as defined in the biosecurity protocol. Adequate implementation of biosecurity measures on farm depends from the least paid and educated workers and is subject to errors during everyday working practice. Therefore daily monitoring of employees' performance is necessary, with continual education, whereby each detected non-compliance serves to improve the existing biosecurity protocol. Since the prevalence of Salmonella spp. on broiler meat at the slaughterhouse, among other factors, depends on Salmonella spp. prevalence on farm, in cases where technological operations are standardized at the slaughterhouse and when the hygiene level is equable, the data on Salmonella spp. prevalence can help us indirectly to assess adequate implementation of biosecurity measures on farm. In our research, after strict and consistent implementation of biosecurity measures on farm, Salmonella spp. prevalence at the slaughterhouse was reduced from 24% to 10%., Glavni ograničavajući faktor uspešnosti proizvodnje mesa brojlera je negativni uticaj potencijalnih bolesti. Biosigurnosne mere predstavljaju način osiguranja bezbednosti od prenosa infektivnih bolesti, parazita i ostalih uzročnika oboljenja. Na nivou primarne proizvodnje (tov brojlera) na farmi, problem predstavlja nedosledna primena biosigurnosnih mera koje su definisane u biosigurnosnom protokolu. Adekvatna primena biosigurnosnih mera na farmi zavisi od najmanje plaćenih i edukovanih radnika i podložna je greškama tokom obavljanja svakodnevne radne prakse. Stoga je potrebno svakodnevno praćenje izvršenja radnih obaveza zaposlenih, uz kontinuiranu edukaciju, pri čemu svaka otkrivena neusaglašenost služi za unapređenje postojećeg biosigurnosnog protokola. Prevalencija Salmonella spp., na mesu brojlera u klanici, između ostalih faktora zavisi i od nalaza Salmonella spp. na farmi. U slučaju kada su tehnološke operacije na liniji klanja standardizovane i postupak sprovođenja higijene ujednačen, podatak o prisustvu Salmonella spp., može poslužiti za procenu da li su primenjene adekvatne biosigurnosne mere na farmi. U našem radu, nakon striktnog i doslednog preduzimanja biosigurnosnih mera na farmi, prevalencija Salmonella spp. na koži vrata brojlera na klanici, smanjena je sa 24% na 10%.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Significance of biosecurity measures in primary broiler production, Značaj biosigurnosnih mera u primarnoj proizvodnji mesa brojlera",
volume = "56",
number = "2",
pages = "91-95",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1246"
}

Rašeta, M., Branković-Lazić, I., Lukić, M., Teodorović, V., Lakićević, B.,& Polaček, V.. (2015). Significance of biosecurity measures in primary broiler production. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 56(2), 91-95.
https://hdl.handle.net/21.15107/rcub_veterinar_1246

Rašeta M, Branković-Lazić I, Lukić M, Teodorović V, Lakićević B, Polaček V. Significance of biosecurity measures in primary broiler production. in Tehnologija mesa. 2015;56(2):91-95.
https://hdl.handle.net/21.15107/rcub_veterinar_1246 .

Rašeta, Mladen, Branković-Lazić, Ivana, Lukić, Mirjana, Teodorović, Vlado, Lakićević, Brankica, Polaček, Vladimir, "Significance of biosecurity measures in primary broiler production" in Tehnologija mesa, 56, no. 2 (2015):91-95,
https://hdl.handle.net/21.15107/rcub_veterinar_1246 .

TY  - JOUR
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Polaček, Vladimir
AU  - Valčić, Miroslav
AU  - Lazić, Gospava
AU  - Pasagić, Enisa
AU  - Petrović, Tamaš
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1180
AB  - The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of equine arteritis virus in the semen of stallions in the republic of Serbia
VL  - 65
IS  - 4
SP  - 557
EP  - 567
DO  - 10.1515/acve-2015-0047
ER  - 

@article{
author = "Lazić, Sava and Lupulović, Diana and Polaček, Vladimir and Valčić, Miroslav and Lazić, Gospava and Pasagić, Enisa and Petrović, Tamaš",
year = "2015",
abstract = "The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia",
volume = "65",
number = "4",
pages = "557-567",
doi = "10.1515/acve-2015-0047"
}

Lazić, S., Lupulović, D., Polaček, V., Valčić, M., Lazić, G., Pasagić, E.,& Petrović, T.. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 557-567.
https://doi.org/10.1515/acve-2015-0047

Lazić S, Lupulović D, Polaček V, Valčić M, Lazić G, Pasagić E, Petrović T. Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):557-567.
doi:10.1515/acve-2015-0047 .

Lazić, Sava, Lupulović, Diana, Polaček, Vladimir, Valčić, Miroslav, Lazić, Gospava, Pasagić, Enisa, Petrović, Tamaš, "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):557-567,
https://doi.org/10.1515/acve-2015-0047 . .

TY  - JOUR
AU  - Rašeta, Mladen
AU  - Teodorović, Vlado
AU  - Bunčić, Olivera
AU  - Katić, Vera
AU  - Branković-Lazić, Ivana
AU  - Polaček, Vladimir
AU  - Vidanović, Dejan
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1140
AB  - During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofl oxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health.
AB  - Tokom 2012. godine sprovedena su istraživanja higijene procesa proizvodnje trupova brojlera, na tri klanice u Republici Srbiji. Ispitano je 150 uzoraka kožica vrata brojlera i utvrđeno je 17 izolata salmonela. Izolati su, korišćenjem odgovarajućih monovalentnih i polivalentnih seruma, tipizovani kao Salmonella enterica subspecies enterica serovar infantis (S. Infantis 6, 7, r, 1, 5). U slučajevima oboljenja ljudi, izolovano je 5 uzoraka salmonele identičnog serovarijeteta. Nakon toga, na 22 uzorka, rađeno je ispitivanje antibiotske rezistencije izolata disk difuzionim testom. Izolati su pokazali rezistentnost prema: ampicilinu i nalidiksičnoj kiselini (95,5%), tetraciklinu (91%), cefotaksim/klavulanskoj kiselini (68,2%), ali ne i prema ciprofl oksacinu, gentamicinu i trimetoprim/sulfametoksazolu (0%). Stepen genetske sličnosti izolata poreklom od obolelih ljudi i sa trupova brojlera je određen molekularnim metodama. Analiza klastera je pokazala prisustvo 7 profi la, dok svi izolati imaju 92% genetske sličnosti. Iako postoje razlike u antimikrobnoj rezistenciji izolata poreklom od obolelih ljudi i sa kožica vrata brojlera, ne može se isključiti epidemiološka povezanost, jer kod dominantnog genotipa SINFXB0001, utvrđenog kod 8 izolata poreklom od obolelih ljudi (3 izolata) i sa kožica vrata brojlera (5 izolata), postoji genetska sličnost od 100%. Na osnovu dobijenih rezultata, istraživanje je pokazalo da prisustvo S. Infantis na trupovima brojlera predstavlja hazard po zdravlje ljudi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses
T1  - Antibiotska rezistencija i molekularna ispitivanja Salmonella enterica subspecies enterica serovar Infantis izolovanih u slučajevima oboljenja ljudi i sa trupova brojlera
VL  - 64
IS  - 2
SP  - 257
EP  - 268
DO  - 10.2478/acve-2014-0024
ER  - 

@article{
author = "Rašeta, Mladen and Teodorović, Vlado and Bunčić, Olivera and Katić, Vera and Branković-Lazić, Ivana and Polaček, Vladimir and Vidanović, Dejan",
year = "2014",
abstract = "During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofl oxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health., Tokom 2012. godine sprovedena su istraživanja higijene procesa proizvodnje trupova brojlera, na tri klanice u Republici Srbiji. Ispitano je 150 uzoraka kožica vrata brojlera i utvrđeno je 17 izolata salmonela. Izolati su, korišćenjem odgovarajućih monovalentnih i polivalentnih seruma, tipizovani kao Salmonella enterica subspecies enterica serovar infantis (S. Infantis 6, 7, r, 1, 5). U slučajevima oboljenja ljudi, izolovano je 5 uzoraka salmonele identičnog serovarijeteta. Nakon toga, na 22 uzorka, rađeno je ispitivanje antibiotske rezistencije izolata disk difuzionim testom. Izolati su pokazali rezistentnost prema: ampicilinu i nalidiksičnoj kiselini (95,5%), tetraciklinu (91%), cefotaksim/klavulanskoj kiselini (68,2%), ali ne i prema ciprofl oksacinu, gentamicinu i trimetoprim/sulfametoksazolu (0%). Stepen genetske sličnosti izolata poreklom od obolelih ljudi i sa trupova brojlera je određen molekularnim metodama. Analiza klastera je pokazala prisustvo 7 profi la, dok svi izolati imaju 92% genetske sličnosti. Iako postoje razlike u antimikrobnoj rezistenciji izolata poreklom od obolelih ljudi i sa kožica vrata brojlera, ne može se isključiti epidemiološka povezanost, jer kod dominantnog genotipa SINFXB0001, utvrđenog kod 8 izolata poreklom od obolelih ljudi (3 izolata) i sa kožica vrata brojlera (5 izolata), postoji genetska sličnost od 100%. Na osnovu dobijenih rezultata, istraživanje je pokazalo da prisustvo S. Infantis na trupovima brojlera predstavlja hazard po zdravlje ljudi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses, Antibiotska rezistencija i molekularna ispitivanja Salmonella enterica subspecies enterica serovar Infantis izolovanih u slučajevima oboljenja ljudi i sa trupova brojlera",
volume = "64",
number = "2",
pages = "257-268",
doi = "10.2478/acve-2014-0024"
}

Rašeta, M., Teodorović, V., Bunčić, O., Katić, V., Branković-Lazić, I., Polaček, V.,& Vidanović, D.. (2014). Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(2), 257-268.
https://doi.org/10.2478/acve-2014-0024

Rašeta M, Teodorović V, Bunčić O, Katić V, Branković-Lazić I, Polaček V, Vidanović D. Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses. in Acta Veterinaria-Beograd. 2014;64(2):257-268.
doi:10.2478/acve-2014-0024 .

Rašeta, Mladen, Teodorović, Vlado, Bunčić, Olivera, Katić, Vera, Branković-Lazić, Ivana, Polaček, Vladimir, Vidanović, Dejan, "Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses" in Acta Veterinaria-Beograd, 64, no. 2 (2014):257-268,
https://doi.org/10.2478/acve-2014-0024 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Prodanov-Radulović, Jasna
AU  - Došen, Radoslav
AU  - Petrović, Tamaš
AU  - Becskei, Zsolt
AU  - Aleksić-Kovačević, Sanja
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1141
AB  - Classical swine fever (CSF) is a highly contagious viral disease of domestic swine and wild boars. The aim of the study was to examine samples of lymphoid tissues and brain for the presence of Classical Swine Fever Virus (CSFV) antigen in piglets that originate from vaccinated and unvaccinated sows, in order to compare the intensity of pathological lesions in conditions of different immunological status. A total of 20 crossbreed piglets of both sexes, aged 45 days were divided in three groups (G1, G2 and G3). Piglets of G1 originated from sows vaccinated with a live vaccine containing attenuated C strain CSFV. Piglets of G2 originated from unvaccinated sows. Two piglets of G3 originated from vaccinated and two piglets from unvaccinated sows and they served as the control group. All the piglets from G1 and G2 were intramuscularly inoculated with 2×105 TCDI/50 of CSFV, Baker strain and they died by the 22nd day post inoculation. Immunohistochemistry was applied for immunolabeling E2 (gp55) glycoprotein of CSFV in the tonsils, mandibular lymph nodes, ileocecal valve and brain of all tested piglets. Differences were observed in the severity of lesions in the lymphoid tissue and the brain between G1 and G2 piglets. The present level of colostral antibodies was not able to protect piglets in G1 from the fatal outcome of the disease. Among others, this finding may also have an impact on vaccination policy in the future. Having in mind that vaccination of all domestic pigs with attenuated C-strain vaccine is still a mandatory control measure for CSF in Serbia, soon a non-vaccination policy should be harmonized with EU directives.
AB  - Klasična kuga svinja (KKS) predstavlja zarazno oboljenje domaćih i divljih svinja. Cilj ovih ispitivanja bio je utvrđivanje prisustva virusa KKS u uzorcima limfatičnog tkiva i mozga prasadi, poreklom od vakcinisanih i nevakcinisanih krmača, i praćenje intenziteta patoloških promena pri različitom imunološkom statusu. Ispitivanja su izvršena na 20 prasadi oba pola, starosti 45 dana, koja su podeljena u tri grupe (G1, G2 i G3). Prasad G1 grupe poticala su od krmača koje su vakcinisane atenuiranom vakcinom C-soja virusa KKS. Prasad G2 i G3 grupe poticala su od nevakcinisanih krmača. Prasad G3 grupe predstavljala su kontrolnu grupu. Virus KKS inokulisan je intramuskularno prasadima G1 i G2 grupe ('Baker' soj, 2×105 TCDI/50). Sva prasad su uginula najdalje do 22. dana od momenta inokulacije. Imunohistohemijska metoda je korišćena za dokazivanje E2 (gp55) antigena u uzorcima tonzila, mandibularnih limfnih čvorova, ileocekalnih tonzila i mozga, kod svih prasadi u ogledu. Utvrđena je razlika u intenzitetu patoloških promena u limfatičnom tkivu i mozgu prasadi G1 i G2 grupe. Kolostralna antitela protiv virusa KKS nisu uspela da zaštite prasad od fatalnog ishoda bolesti. Između ostalog, ovi rezultati mogu imati značaj za program vakcinacije u budućnosti. Naime, vakcinacija svinja protiv KKS, atenuiranim K sojem virusa i dalje je obavezna zakonska mera u Srbiji. Uskoro će se program prestanka vakcinacije uskladiti sa EU direktivama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Expression of E2 (gp 55) glycoprotein of classical swine fever virus in lymphoid tissue and brain of experimentally infected piglets with different immunological status
T1  - Ekspresija E2 (gp 55) antigena virusa klasične kuge svinja u limfatičnom tkivu i mozgu eksperimentalno inficirane prasadi sa različitim imunološkim statusom
VL  - 64
IS  - 2
SP  - 213
EP  - 225
DO  - 10.2478/acve-2014-0020
ER  - 

@article{
author = "Polaček, Vladimir and Prodanov-Radulović, Jasna and Došen, Radoslav and Petrović, Tamaš and Becskei, Zsolt and Aleksić-Kovačević, Sanja",
year = "2014",
abstract = "Classical swine fever (CSF) is a highly contagious viral disease of domestic swine and wild boars. The aim of the study was to examine samples of lymphoid tissues and brain for the presence of Classical Swine Fever Virus (CSFV) antigen in piglets that originate from vaccinated and unvaccinated sows, in order to compare the intensity of pathological lesions in conditions of different immunological status. A total of 20 crossbreed piglets of both sexes, aged 45 days were divided in three groups (G1, G2 and G3). Piglets of G1 originated from sows vaccinated with a live vaccine containing attenuated C strain CSFV. Piglets of G2 originated from unvaccinated sows. Two piglets of G3 originated from vaccinated and two piglets from unvaccinated sows and they served as the control group. All the piglets from G1 and G2 were intramuscularly inoculated with 2×105 TCDI/50 of CSFV, Baker strain and they died by the 22nd day post inoculation. Immunohistochemistry was applied for immunolabeling E2 (gp55) glycoprotein of CSFV in the tonsils, mandibular lymph nodes, ileocecal valve and brain of all tested piglets. Differences were observed in the severity of lesions in the lymphoid tissue and the brain between G1 and G2 piglets. The present level of colostral antibodies was not able to protect piglets in G1 from the fatal outcome of the disease. Among others, this finding may also have an impact on vaccination policy in the future. Having in mind that vaccination of all domestic pigs with attenuated C-strain vaccine is still a mandatory control measure for CSF in Serbia, soon a non-vaccination policy should be harmonized with EU directives., Klasična kuga svinja (KKS) predstavlja zarazno oboljenje domaćih i divljih svinja. Cilj ovih ispitivanja bio je utvrđivanje prisustva virusa KKS u uzorcima limfatičnog tkiva i mozga prasadi, poreklom od vakcinisanih i nevakcinisanih krmača, i praćenje intenziteta patoloških promena pri različitom imunološkom statusu. Ispitivanja su izvršena na 20 prasadi oba pola, starosti 45 dana, koja su podeljena u tri grupe (G1, G2 i G3). Prasad G1 grupe poticala su od krmača koje su vakcinisane atenuiranom vakcinom C-soja virusa KKS. Prasad G2 i G3 grupe poticala su od nevakcinisanih krmača. Prasad G3 grupe predstavljala su kontrolnu grupu. Virus KKS inokulisan je intramuskularno prasadima G1 i G2 grupe ('Baker' soj, 2×105 TCDI/50). Sva prasad su uginula najdalje do 22. dana od momenta inokulacije. Imunohistohemijska metoda je korišćena za dokazivanje E2 (gp55) antigena u uzorcima tonzila, mandibularnih limfnih čvorova, ileocekalnih tonzila i mozga, kod svih prasadi u ogledu. Utvrđena je razlika u intenzitetu patoloških promena u limfatičnom tkivu i mozgu prasadi G1 i G2 grupe. Kolostralna antitela protiv virusa KKS nisu uspela da zaštite prasad od fatalnog ishoda bolesti. Između ostalog, ovi rezultati mogu imati značaj za program vakcinacije u budućnosti. Naime, vakcinacija svinja protiv KKS, atenuiranim K sojem virusa i dalje je obavezna zakonska mera u Srbiji. Uskoro će se program prestanka vakcinacije uskladiti sa EU direktivama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Expression of E2 (gp 55) glycoprotein of classical swine fever virus in lymphoid tissue and brain of experimentally infected piglets with different immunological status, Ekspresija E2 (gp 55) antigena virusa klasične kuge svinja u limfatičnom tkivu i mozgu eksperimentalno inficirane prasadi sa različitim imunološkim statusom",
volume = "64",
number = "2",
pages = "213-225",
doi = "10.2478/acve-2014-0020"
}

Polaček, V., Prodanov-Radulović, J., Došen, R., Petrović, T., Becskei, Z.,& Aleksić-Kovačević, S.. (2014). Expression of E2 (gp 55) glycoprotein of classical swine fever virus in lymphoid tissue and brain of experimentally infected piglets with different immunological status. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(2), 213-225.
https://doi.org/10.2478/acve-2014-0020

Polaček V, Prodanov-Radulović J, Došen R, Petrović T, Becskei Z, Aleksić-Kovačević S. Expression of E2 (gp 55) glycoprotein of classical swine fever virus in lymphoid tissue and brain of experimentally infected piglets with different immunological status. in Acta Veterinaria-Beograd. 2014;64(2):213-225.
doi:10.2478/acve-2014-0020 .

Polaček, Vladimir, Prodanov-Radulović, Jasna, Došen, Radoslav, Petrović, Tamaš, Becskei, Zsolt, Aleksić-Kovačević, Sanja, "Expression of E2 (gp 55) glycoprotein of classical swine fever virus in lymphoid tissue and brain of experimentally infected piglets with different immunological status" in Acta Veterinaria-Beograd, 64, no. 2 (2014):213-225,
https://doi.org/10.2478/acve-2014-0020 . .

TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Došen, Radoslav
AU  - Polaček, Vladimir
AU  - Petrović, Tamaš
AU  - Stojanov, Igor
AU  - Ratajac, Radomir
AU  - Valčić, Miroslav
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1137
AB  - The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions.
AB  - Cilj istraživanja je bio da se utvrdi efikasnost vakcinacije subjedičnom vakcinom protiv infekcije virulentnim virusom KKS kod prasadi poreklom od krmača vakcinisanih Kina sojem. Eksperimenatalna ispitivanja su obavljena na 34 praseta uzrasta 45 dana (13 neimunih i 21 prase poreklom od krmača vakcinisanih Kina sojem). Na osnovu rezultata seroloških ispitivanja prasadi uzrasta 40 dana, formirane su tri eksperimentalne grupe od po sedam prasadi u svakoj. Prasad su vakcinisana subjediničnom vakcinom u uzrastu od 45 dana. Nakon revakcinacije, jedinke su veštački inficirane virulentnim sojem virusa KKS. U cilju kontrole izlučivanja virusa, u svaku eksperimentalnu grupu su dodana po dva neimuna praseta. Nakon veštačke infekcije, vršen je klinički pregled prasadi i uzorkovanje krvi od svake životinje u cilju utvrđivanja prisustva antitela protiv virusa KKS i viremije. Kontrola izlučivanja virusa KKS iz organizma vakcinisanih, veštački inficiranih prasadi je vršena tehnikom RT-PCR na uzorcima orofaringealnih i rektalnih briseva. Nakon uginuća ili žrtvovanja, vršen je patomorfološki pregled i uzorkovanje tkiva u cilju utvrđivanje prisustva i distribucije antigena virusa KKS u uzorcima tkiva (ELISA test i RT-PCR). Vakcinacijom prasadi poreklom od krmača vakcinisanih Kina-sojem utvrđen je solidan aktivni imunski odgovor. Nakon veštačke infekcije utvrđeno je izlučivanje virusa ali bez kontaktne infekcije prijemčivih jedinki u kohabitaciji. Rezultati ukazuju da subjedinična vakcina može imati potencijalnu primenu u kontroli KKS u endemski inficiranim područjima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Classical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strain
T1  - Klasična kuga svinja - aktivna imunizacija prasadi subjediničnom (E2) vakcinom u prisustvu različitog nivoa kolostralnog imuniteta
VL  - 64
IS  - 4
SP  - 493
EP  - 509
DO  - 10.2478/acve-2014-0046
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Došen, Radoslav and Polaček, Vladimir and Petrović, Tamaš and Stojanov, Igor and Ratajac, Radomir and Valčić, Miroslav",
year = "2014",
abstract = "The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions., Cilj istraživanja je bio da se utvrdi efikasnost vakcinacije subjedičnom vakcinom protiv infekcije virulentnim virusom KKS kod prasadi poreklom od krmača vakcinisanih Kina sojem. Eksperimenatalna ispitivanja su obavljena na 34 praseta uzrasta 45 dana (13 neimunih i 21 prase poreklom od krmača vakcinisanih Kina sojem). Na osnovu rezultata seroloških ispitivanja prasadi uzrasta 40 dana, formirane su tri eksperimentalne grupe od po sedam prasadi u svakoj. Prasad su vakcinisana subjediničnom vakcinom u uzrastu od 45 dana. Nakon revakcinacije, jedinke su veštački inficirane virulentnim sojem virusa KKS. U cilju kontrole izlučivanja virusa, u svaku eksperimentalnu grupu su dodana po dva neimuna praseta. Nakon veštačke infekcije, vršen je klinički pregled prasadi i uzorkovanje krvi od svake životinje u cilju utvrđivanja prisustva antitela protiv virusa KKS i viremije. Kontrola izlučivanja virusa KKS iz organizma vakcinisanih, veštački inficiranih prasadi je vršena tehnikom RT-PCR na uzorcima orofaringealnih i rektalnih briseva. Nakon uginuća ili žrtvovanja, vršen je patomorfološki pregled i uzorkovanje tkiva u cilju utvrđivanje prisustva i distribucije antigena virusa KKS u uzorcima tkiva (ELISA test i RT-PCR). Vakcinacijom prasadi poreklom od krmača vakcinisanih Kina-sojem utvrđen je solidan aktivni imunski odgovor. Nakon veštačke infekcije utvrđeno je izlučivanje virusa ali bez kontaktne infekcije prijemčivih jedinki u kohabitaciji. Rezultati ukazuju da subjedinična vakcina može imati potencijalnu primenu u kontroli KKS u endemski inficiranim područjima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Classical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strain, Klasična kuga svinja - aktivna imunizacija prasadi subjediničnom (E2) vakcinom u prisustvu različitog nivoa kolostralnog imuniteta",
volume = "64",
number = "4",
pages = "493-509",
doi = "10.2478/acve-2014-0046"
}

Prodanov-Radulović, J., Došen, R., Polaček, V., Petrović, T., Stojanov, I., Ratajac, R.,& Valčić, M.. (2014). Classical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strain. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(4), 493-509.
https://doi.org/10.2478/acve-2014-0046

Prodanov-Radulović J, Došen R, Polaček V, Petrović T, Stojanov I, Ratajac R, Valčić M. Classical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strain. in Acta Veterinaria-Beograd. 2014;64(4):493-509.
doi:10.2478/acve-2014-0046 .

Prodanov-Radulović, Jasna, Došen, Radoslav, Polaček, Vladimir, Petrović, Tamaš, Stojanov, Igor, Ratajac, Radomir, Valčić, Miroslav, "Classical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strain" in Acta Veterinaria-Beograd, 64, no. 4 (2014):493-509,
https://doi.org/10.2478/acve-2014-0046 . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/945
AB  - Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Application of standard and molecular methods for the diagnosis of Newcastle disease
VL  - 64
IS  - 4
SP  - 1433
EP  - 1437
DO  - 10.2298/ABS1204433V
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Polaček, Vladimir and Vasković, Nikola and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov",
year = "2012",
abstract = "Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Application of standard and molecular methods for the diagnosis of Newcastle disease",
volume = "64",
number = "4",
pages = "1433-1437",
doi = "10.2298/ABS1204433V"
}

Vidanović, D., Šekler, M., Polaček, V., Vasković, N., Ašanin, R., Milić, N.,& Nišavić, J.. (2012). Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1433-1437.
https://doi.org/10.2298/ABS1204433V

Vidanović D, Šekler M, Polaček V, Vasković N, Ašanin R, Milić N, Nišavić J. Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences. 2012;64(4):1433-1437.
doi:10.2298/ABS1204433V .

Vidanović, Dejan, Šekler, Milanko, Polaček, Vladimir, Vasković, Nikola, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, "Application of standard and molecular methods for the diagnosis of Newcastle disease" in Archives of Biological Sciences, 64, no. 4 (2012):1433-1437,
https://doi.org/10.2298/ABS1204433V . .