Milčić-Matić, Natalija

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Authority KeyName Variants
orcid::0000-0001-6857-8042
  • Milčić-Matić, Natalija (16)
  • Milčić Matić, Natalija (7)
Projects
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200143 (University of Belgrade, Faculty of Veterinary Medicine) Molecular properties and modifications of some respiratory and nutritional allergens
Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production Bernard Osher Initiative for Research on Severe Asthma at Karolinska InstitutetKarolinska Institutet
Center for Inflammatory Diseases Centre for Allergy Research
EAACI Hesselman Foundation
Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200053 (University of Belgrade, Institute for Multidisciplinary Research) Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima
Development of herbal medicines and biocides on the basis of Carvacrol, Thymol and Cinnamaldehyde for use in veterinary medicine, animal husbandry and food production without harmful residues Karolinska InstitutetKarolinska Institutet
King Gustaf V 80th Birthday Foundation Konsul Th C Bergh Foundation
Stockholm County CouncilStockholm County Council Swedish Asthma and Allergy Association's Research Foundation
Swedish Cancer and Allergy Foundation Swedish Heart-Lung FoundationSwedish Heart-Lung Foundation
Swedish Research CouncilSwedish Research Council

Author's Bibliography

Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa

Prošić, Isidora; Milčić-Matić, Natalija; Radalj, Andrea; Nikšić, Aleksandar; Ilić, Milica; Krnjaić, Dejan

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2024)

TY  - CONF
AU  - Prošić, Isidora
AU  - Milčić-Matić, Natalija
AU  - Radalj, Andrea
AU  - Nikšić, Aleksandar
AU  - Ilić, Milica
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3879
AB  - Mетицилин-резистентнe стафилококe (МРС) представљају значајан изазов у лечењу бактеријских инфекција кућних љубимаца и у великој мери ограничавају терапијске могућности. Осим тога, ови сојеви су значајна претња по јавно здравље на глобалном нивоу. Спроведена студија имала је за циљ да истражи молекуларну преваленцију коагулазa-позитивних стафилокока (КоПС) које изазивају инфекције коже и ушију код паса и да идентификује МРС из групе КоПС. Из узорака пореклом од паса са инфекцијом ушију и/или коже пристиглих са Клинике за Дерматологију Факултета ветеринарске медицине изоловано је укупно 79 сојева из рода Staphylococcus. На основу разноликости секвенце nuc гена, применом Multiplex PCR методе детектоване су врсте из групе КоПС. Фенотипска резистенција МРС доказана је методом диск-дифузије и одређивањем вредности минималне инхибиторне концентрације, присуство PBP2a (penicillin-binding protein 2a, енгл.) доказано је применом латекс аглутинације, док је молекуларном детекцијом испитано присуство mecA и mecC гена. Међу 76 КоПС изолата, 50/76 (65,8%) је идентификовано као S. pseudintermedius, 17/76 (22,4%) као S. aureus, 6/76 (7,9%) као S. coagulans и 3/76 (3,9%) %) као S. intermedius. Присуство mecA гена је детектовано у 30% (15/50) изолата S. pseudintermedius и 29,4% (5/17) изолата S. aureus који су класификовани као метицилин-резистентне S. pseudintermedius (МРSP) и метицилин-резистентне S. аureus (MRSA). MecC ген није детектован код ни једног изолата. Представљени налази наглашавају високу преваленцију МРС-а изолованих из ушију и коже паса на територији Београда, истичући важност континуираног надзора као првог корака у борби против МРС-а и контролних мера за смањење ширења антимикробне резистенције (АМР). Овакве и сличне студије су важне у решавању проблема АМР и обезбеђивању ефикасних стратегија лечења како животиња тако и људи, имајући у виду све већи број студија које поред MRSA, детектују сојеве S. pseudintermedius и МRSP као опортунистичке патогене за људе и узрочнике емергентних зооноза.
AB  - Methicillin-resistant staphylococci (MRS) limit therapeutic options and pose a significant challenge in the treatment of bacterial infections in companion animals. Moreover, these strains pose a significant threat to global public health. This study aimed to investigate the molecular prevalence of coagulase-positive staphylococci (CoPS) causing skin and ear infections in dogs and to identify methicillin-resistant CoPS. A total of 79 staphylococcal strains were isolated from clinical samples collected from dogs with suspected skin and ear infections from the Dermatology clinic at the Faculty of Veterinary Medicine. Multiplex PCR was employed for species-specific identification of CoPS based on the sequence diversity of the nuc gene. Phenotypic characterization of methicilin resistance was performed using disc-diffusion and determination of minimally inhibitory concentrations values, while PBP2a (penicillin-binding protein 2a) was detected using latex agglutination and finally molecular detection of mecA and mecC genes was performed. Among the 76 CoPS isolates, 50/76 (65.8%) were identified as S. pseudintermedius, 17/76 (22.4%) as S. aureus, 6/76 (7.9%) as S. coagulans, and 3/76 (3.9%) as S. intermedius. The mecA gene was detected in 30% (15/50) of S. pseudintermedius isolates and 29.4% (5/17) of S. aureus isolates, classified as methicillin-resistant S. pseudintermedius (MRSP) and methicillin-resistant S. aureus (MRSA), respectively. No isolates tested positive for the mecC gene. These findings underscore the high prevalence of MRS in canine dermatological infections, highlighting the importance of continuous monitoring as the first step in combating the high prevalence of MRS and robust infection control measures to mitigate the spread of antimicrobial resistance (AMR). Such efforts are crucial in addressing the public health implications associated with AMR and ensuring effective treatment strategies for both humans and animals considering that besides MRSA, newer studies recognize S. pseudintermedius and MRSP strains as opportunistic human pathogens and emerging zoonotic agents.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa
T1  - Molecular prevalence of methicillin-resistant coagulase-positive staphylococci isolated from  canine skin and ears
SP  - 182
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3879
ER  - 
@conference{
author = "Prošić, Isidora and Milčić-Matić, Natalija and Radalj, Andrea and Nikšić, Aleksandar and Ilić, Milica and Krnjaić, Dejan",
year = "2024",
abstract = "Mетицилин-резистентнe стафилококe (МРС) представљају значајан изазов у лечењу бактеријских инфекција кућних љубимаца и у великој мери ограничавају терапијске могућности. Осим тога, ови сојеви су значајна претња по јавно здравље на глобалном нивоу. Спроведена студија имала је за циљ да истражи молекуларну преваленцију коагулазa-позитивних стафилокока (КоПС) које изазивају инфекције коже и ушију код паса и да идентификује МРС из групе КоПС. Из узорака пореклом од паса са инфекцијом ушију и/или коже пристиглих са Клинике за Дерматологију Факултета ветеринарске медицине изоловано је укупно 79 сојева из рода Staphylococcus. На основу разноликости секвенце nuc гена, применом Multiplex PCR методе детектоване су врсте из групе КоПС. Фенотипска резистенција МРС доказана је методом диск-дифузије и одређивањем вредности минималне инхибиторне концентрације, присуство PBP2a (penicillin-binding protein 2a, енгл.) доказано је применом латекс аглутинације, док је молекуларном детекцијом испитано присуство mecA и mecC гена. Међу 76 КоПС изолата, 50/76 (65,8%) је идентификовано као S. pseudintermedius, 17/76 (22,4%) као S. aureus, 6/76 (7,9%) као S. coagulans и 3/76 (3,9%) %) као S. intermedius. Присуство mecA гена је детектовано у 30% (15/50) изолата S. pseudintermedius и 29,4% (5/17) изолата S. aureus који су класификовани као метицилин-резистентне S. pseudintermedius (МРSP) и метицилин-резистентне S. аureus (MRSA). MecC ген није детектован код ни једног изолата. Представљени налази наглашавају високу преваленцију МРС-а изолованих из ушију и коже паса на територији Београда, истичући важност континуираног надзора као првог корака у борби против МРС-а и контролних мера за смањење ширења антимикробне резистенције (АМР). Овакве и сличне студије су важне у решавању проблема АМР и обезбеђивању ефикасних стратегија лечења како животиња тако и људи, имајући у виду све већи број студија које поред MRSA, детектују сојеве S. pseudintermedius и МRSP као опортунистичке патогене за људе и узрочнике емергентних зооноза., Methicillin-resistant staphylococci (MRS) limit therapeutic options and pose a significant challenge in the treatment of bacterial infections in companion animals. Moreover, these strains pose a significant threat to global public health. This study aimed to investigate the molecular prevalence of coagulase-positive staphylococci (CoPS) causing skin and ear infections in dogs and to identify methicillin-resistant CoPS. A total of 79 staphylococcal strains were isolated from clinical samples collected from dogs with suspected skin and ear infections from the Dermatology clinic at the Faculty of Veterinary Medicine. Multiplex PCR was employed for species-specific identification of CoPS based on the sequence diversity of the nuc gene. Phenotypic characterization of methicilin resistance was performed using disc-diffusion and determination of minimally inhibitory concentrations values, while PBP2a (penicillin-binding protein 2a) was detected using latex agglutination and finally molecular detection of mecA and mecC genes was performed. Among the 76 CoPS isolates, 50/76 (65.8%) were identified as S. pseudintermedius, 17/76 (22.4%) as S. aureus, 6/76 (7.9%) as S. coagulans, and 3/76 (3.9%) as S. intermedius. The mecA gene was detected in 30% (15/50) of S. pseudintermedius isolates and 29.4% (5/17) of S. aureus isolates, classified as methicillin-resistant S. pseudintermedius (MRSP) and methicillin-resistant S. aureus (MRSA), respectively. No isolates tested positive for the mecC gene. These findings underscore the high prevalence of MRS in canine dermatological infections, highlighting the importance of continuous monitoring as the first step in combating the high prevalence of MRS and robust infection control measures to mitigate the spread of antimicrobial resistance (AMR). Such efforts are crucial in addressing the public health implications associated with AMR and ensuring effective treatment strategies for both humans and animals considering that besides MRSA, newer studies recognize S. pseudintermedius and MRSP strains as opportunistic human pathogens and emerging zoonotic agents.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa, Molecular prevalence of methicillin-resistant coagulase-positive staphylococci isolated from  canine skin and ears",
pages = "182-183",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3879"
}
Prošić, I., Milčić-Matić, N., Radalj, A., Nikšić, A., Ilić, M.,& Krnjaić, D.. (2024). Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3879
Prošić I, Milčić-Matić N, Radalj A, Nikšić A, Ilić M, Krnjaić D. Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3879 .
Prošić, Isidora, Milčić-Matić, Natalija, Radalj, Andrea, Nikšić, Aleksandar, Ilić, Milica, Krnjaić, Dejan, "Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):182-183,
https://hdl.handle.net/21.15107/rcub_veterinar_3879 .

Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study

Prošić, Isidora; Milčić Matić, Natalija; Milić, Nenad; Radalj, Andrea; Aksentijević, Ksenija; Ilić, Milica; Nišavić, Jakov; Radojičić, Marina; Gajdov, Vladimir; Krnjaić, Dejan

(Beograd : Fakultet veterinarske medicine, 2024)

TY  - JOUR
AU  - Prošić, Isidora
AU  - Milčić Matić, Natalija
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Aksentijević, Ksenija
AU  - Ilić, Milica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Gajdov, Vladimir
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3763
AB  - The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study
VL  - 74
IS  - 1
SP  - 117
EP  - 132
DO  - 10.2478/acve-2024-0009
DO  - 10.2478/acve-2024-0009
ER  - 
@article{
author = "Prošić, Isidora and Milčić Matić, Natalija and Milić, Nenad and Radalj, Andrea and Aksentijević, Ksenija and Ilić, Milica and Nišavić, Jakov and Radojičić, Marina and Gajdov, Vladimir and Krnjaić, Dejan",
year = "2024",
abstract = "The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study",
volume = "74",
number = "1",
pages = "117-132",
doi = "10.2478/acve-2024-0009, 10.2478/acve-2024-0009"
}
Prošić, I., Milčić Matić, N., Milić, N., Radalj, A., Aksentijević, K., Ilić, M., Nišavić, J., Radojičić, M., Gajdov, V.,& Krnjaić, D.. (2024). Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 117-132.
https://doi.org/10.2478/acve-2024-0009
Prošić I, Milčić Matić N, Milić N, Radalj A, Aksentijević K, Ilić M, Nišavić J, Radojičić M, Gajdov V, Krnjaić D. Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria. 2024;74(1):117-132.
doi:10.2478/acve-2024-0009 .
Prošić, Isidora, Milčić Matić, Natalija, Milić, Nenad, Radalj, Andrea, Aksentijević, Ksenija, Ilić, Milica, Nišavić, Jakov, Radojičić, Marina, Gajdov, Vladimir, Krnjaić, Dejan, "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study" in Acta Veterinaria, 74, no. 1 (2024):117-132,
https://doi.org/10.2478/acve-2024-0009 . .

Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study

Spasojević, Dragica; Zmejkoski, Danica; Milčić Matić, Natalija; Krnjaić, Dejan; Radotić, Ksenija

(Springer, 2024)

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3702
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
VL  - 48
IS  - 1
SP  - 591
EP  - 596
DO  - 10.1007/s11259-023-10224-9
ER  - 
@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2024",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
volume = "48",
number = "1",
pages = "591-596",
doi = "10.1007/s11259-023-10224-9"
}
Spasojević, D., Zmejkoski, D., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2024). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications
Springer., 48(1), 591-596.
https://doi.org/10.1007/s11259-023-10224-9
Spasojević D, Zmejkoski D, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2024;48(1):591-596.
doi:10.1007/s11259-023-10224-9 .
Spasojević, Dragica, Zmejkoski, Danica, Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications, 48, no. 1 (2024):591-596,
https://doi.org/10.1007/s11259-023-10224-9 . .

Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase)

Kovačević Filipović, Milica; Francuski Andrić, Jelena; Ilić Božović, Anja; Milčić Matić, Natalija

(Beograd : Fakultet veterinarske medicine, 2023)

TY  - CONF
AU  - Kovačević Filipović, Milica
AU  - Francuski Andrić, Jelena
AU  - Ilić Božović, Anja
AU  - Milčić Matić, Natalija
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3310
AB  - Tankoigledna biopsija je sigurna i brza metoda koja je laka za izođenje.
Preporučuje se kao dijagnostička metoda za sve uvećane limfne čvorove, bilo da
se radi o uni- ili multicentričnim promenama kao i za sve kutane i subkutane
mase. Osim tankoiglene biopsije, po ekstirpaciji limfnog čvora, sa njegovog
preseka se može uraditi preparat – otisak.
Citološkim pregledom limfnog čvora se može utvrditi da li postoji
reaktivna hiperplazija, inflamatorne promena u tipu limfadenitisa, primarna
neoplazija limfnog čvora, infiltracija metastatskim tumorskim ćelijama ili
ekstramedularna hematopoeza.
Svaka od navedenih patoloških promena ima određene citološke
karakteristike. Preparat se pregleda na malom uveličanju da bi se uočila
celularnost, raspored ćelija, eventualne metastatske ćelije i optimalno mesto za
pregled. Potrebno je prepoznati tip pozadinskog (engl. background) materijala
primenjujući prvo malo, a potom i veliko uveličanje. To je materijal koji ne
predstavlja patološke ćelije ispitivanog tkiva/mase, već eritrocite koji dospevaju
na preparat oštećenjem krvnih sudova prilikom pravljenja preparata i/ili
ekstracelularni matriks, i/ili specifičan ekstraćelijski materijal koji može biti
tipičan za određeno tkivo i patološku promenu. Na primer, limfoglandularna
telašca predstavljaju delimično razložene delove apoptotičnih limfocita,
karakterističnog su izgleda i javljaju se u većem broju kod limfoma. Potom,
citološki nalaz uključuje prepoznavanje i opis tipova ćelija koje se vide, njihovu
morfologiju i međusobni odnos.
Osnovna pravila prilikom primene tankoiglene biopsije u pravljenju i
tumačenju citoloških preparata limfnih čvorova su sledeća: treba izbegavati da se
analiziraju submandibularni limfni čvorovi koji vrlo često imaju reaktivne promene. Ukoliko postoji generalizovana limfadenopatija treba uzeti uzorke iz
najmanje dva ili svih limfnih čvorova. Igla promera 22G treba da bude plasirana u
nekoliko pravaca i tangencionalno u odnosu na centar limfnog čvora. Kao
problem se može javiti aspiracija tkiva mimo limfnog čvora, a u
submandibularnom regionu su to često pljuvačne žlezde. Takođe, limitacija
citološke dijagnostike postoji kod sitnoćelijskih limfoma mačaka kada su limfociti
dobro diferencirani ili u slučaju fokalne distribucije metastaza. Stepen slaganja u
dijagnostici i prognozi citoloških interpretacija u odnosu na patohistološke zavisi
od tipa limfoma ili metastatske promene.
Kožni ili potkožni noduli su relativno česti problemi kod pasa i mačaka i
mogu imati različitu etiologiju. Dijagnostički pristup se sastoji od uzimanja
detaljne istorije bolesti, pre svega da li je promena nastala naglo ili je prisutna
duži vremenski period. Brzina rasta je takodje od velike važnosti, kao i sam
klinički izgled promene, kao i da li su dobro ograničene. Dubina lezije takođe
može biti od pomoći za izradu diferencijalne liste i dijagnostičkog plana. Ponovno
pojavljanje lezije u oblastima gde je izvršena operacija najčešće ukazuje na
neoplastičnu tvorevinu. Kod starijih pasa česte su potpuno benigne izrasline sa
karakterističnim izgledom. Pozitivan odgovor na antibiotike često ukazuje na
infektivnu etiologiju, ali ne isključuje mogućnost istovremenih bolesti kao što su
dermatomikoze ili tumori.
Prva diferencijacija u proceni nodula je da se utvrdi ili isključi postojanje
neoplastičnih promena za razliku od inflamatornih uzroka ili hiperplazije tkiva. U
te svrhe se koristi tankoiglena biopsija, kako bi se identifikovale prisutne ćelije. U
velikom broju slučajeva kliničar na osnovu mikroskopskog pregleda pripremljenih
pločica, može da proceni da li je u pitanju neoplazija, hiperplazija ili inflamatorni
proces. Na osnovu toga ili preuzima adekvatnu terapiju ili sprovodi dodatne
dijagnostičke postupke.
Sveobuhvatno gledano, sa pojedinim izuzecima, citologija predstavlja
brzu, jednostavnu i pouzdanu metodu za dijagnostiku inflamatornih i
neoplastičnih promena limfnih čvorova, kao i nodula na koži.
PB  - Beograd : Fakultet veterinarske medicine
C3  - 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
T1  - Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase)
VL  - 34
IS  - 33
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3310
ER  - 
@conference{
author = "Kovačević Filipović, Milica and Francuski Andrić, Jelena and Ilić Božović, Anja and Milčić Matić, Natalija",
year = "2023",
abstract = "Tankoigledna biopsija je sigurna i brza metoda koja je laka za izođenje.
Preporučuje se kao dijagnostička metoda za sve uvećane limfne čvorove, bilo da
se radi o uni- ili multicentričnim promenama kao i za sve kutane i subkutane
mase. Osim tankoiglene biopsije, po ekstirpaciji limfnog čvora, sa njegovog
preseka se može uraditi preparat – otisak.
Citološkim pregledom limfnog čvora se može utvrditi da li postoji
reaktivna hiperplazija, inflamatorne promena u tipu limfadenitisa, primarna
neoplazija limfnog čvora, infiltracija metastatskim tumorskim ćelijama ili
ekstramedularna hematopoeza.
Svaka od navedenih patoloških promena ima određene citološke
karakteristike. Preparat se pregleda na malom uveličanju da bi se uočila
celularnost, raspored ćelija, eventualne metastatske ćelije i optimalno mesto za
pregled. Potrebno je prepoznati tip pozadinskog (engl. background) materijala
primenjujući prvo malo, a potom i veliko uveličanje. To je materijal koji ne
predstavlja patološke ćelije ispitivanog tkiva/mase, već eritrocite koji dospevaju
na preparat oštećenjem krvnih sudova prilikom pravljenja preparata i/ili
ekstracelularni matriks, i/ili specifičan ekstraćelijski materijal koji može biti
tipičan za određeno tkivo i patološku promenu. Na primer, limfoglandularna
telašca predstavljaju delimično razložene delove apoptotičnih limfocita,
karakterističnog su izgleda i javljaju se u većem broju kod limfoma. Potom,
citološki nalaz uključuje prepoznavanje i opis tipova ćelija koje se vide, njihovu
morfologiju i međusobni odnos.
Osnovna pravila prilikom primene tankoiglene biopsije u pravljenju i
tumačenju citoloških preparata limfnih čvorova su sledeća: treba izbegavati da se
analiziraju submandibularni limfni čvorovi koji vrlo često imaju reaktivne promene. Ukoliko postoji generalizovana limfadenopatija treba uzeti uzorke iz
najmanje dva ili svih limfnih čvorova. Igla promera 22G treba da bude plasirana u
nekoliko pravaca i tangencionalno u odnosu na centar limfnog čvora. Kao
problem se može javiti aspiracija tkiva mimo limfnog čvora, a u
submandibularnom regionu su to često pljuvačne žlezde. Takođe, limitacija
citološke dijagnostike postoji kod sitnoćelijskih limfoma mačaka kada su limfociti
dobro diferencirani ili u slučaju fokalne distribucije metastaza. Stepen slaganja u
dijagnostici i prognozi citoloških interpretacija u odnosu na patohistološke zavisi
od tipa limfoma ili metastatske promene.
Kožni ili potkožni noduli su relativno česti problemi kod pasa i mačaka i
mogu imati različitu etiologiju. Dijagnostički pristup se sastoji od uzimanja
detaljne istorije bolesti, pre svega da li je promena nastala naglo ili je prisutna
duži vremenski period. Brzina rasta je takodje od velike važnosti, kao i sam
klinički izgled promene, kao i da li su dobro ograničene. Dubina lezije takođe
može biti od pomoći za izradu diferencijalne liste i dijagnostičkog plana. Ponovno
pojavljanje lezije u oblastima gde je izvršena operacija najčešće ukazuje na
neoplastičnu tvorevinu. Kod starijih pasa česte su potpuno benigne izrasline sa
karakterističnim izgledom. Pozitivan odgovor na antibiotike često ukazuje na
infektivnu etiologiju, ali ne isključuje mogućnost istovremenih bolesti kao što su
dermatomikoze ili tumori.
Prva diferencijacija u proceni nodula je da se utvrdi ili isključi postojanje
neoplastičnih promena za razliku od inflamatornih uzroka ili hiperplazije tkiva. U
te svrhe se koristi tankoiglena biopsija, kako bi se identifikovale prisutne ćelije. U
velikom broju slučajeva kliničar na osnovu mikroskopskog pregleda pripremljenih
pločica, može da proceni da li je u pitanju neoplazija, hiperplazija ili inflamatorni
proces. Na osnovu toga ili preuzima adekvatnu terapiju ili sprovodi dodatne
dijagnostičke postupke.
Sveobuhvatno gledano, sa pojedinim izuzecima, citologija predstavlja
brzu, jednostavnu i pouzdanu metodu za dijagnostiku inflamatornih i
neoplastičnih promena limfnih čvorova, kao i nodula na koži.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023",
title = "Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase)",
volume = "34",
number = "33",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3310"
}
Kovačević Filipović, M., Francuski Andrić, J., Ilić Božović, A.,& Milčić Matić, N.. (2023). Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase). in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
Beograd : Fakultet veterinarske medicine., 34(33).
https://hdl.handle.net/21.15107/rcub_veterinar_3310
Kovačević Filipović M, Francuski Andrić J, Ilić Božović A, Milčić Matić N. Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase). in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023. 2023;34(33).
https://hdl.handle.net/21.15107/rcub_veterinar_3310 .
Kovačević Filipović, Milica, Francuski Andrić, Jelena, Ilić Božović, Anja, Milčić Matić, Natalija, "Citologija u svakodnevnoj kliničkoj praksi (limfni čvorovi, kutane i subkutane mase)" in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023, 34, no. 33 (2023),
https://hdl.handle.net/21.15107/rcub_veterinar_3310 .

Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici

Marinković, Darko; Milčić Matić, Natalija; Jelisić, Stefan; Aničić, Milan

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2023)

TY  - CONF
AU  - Marinković, Darko
AU  - Milčić Matić, Natalija
AU  - Jelisić, Stefan
AU  - Aničić, Milan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3061
AB  - Царство гљива (Funghi) обухвата 6 милиона врста у оквиру којих се срећу облици
којима је развијен талус – гљиве/печурке и микроскопске облике – гљивице и
кваснице од којих 625 врста може да изазове инфекцију кичмењака и поред
тренутно актуелних вирусних и бактеријских обољења представљају све значајнија
обољења глобалног карактера која често имају зоонотски карактер.
Микозе (гљивичне инфекције) представљају обољења изазвана гљивицама.
Гљивице могу да инфицирају различитa ткива изазивајући површинске или дубоке
патолошке промене као и системско обољење. Микозе се деле на сапронозе
(сапронозне гљивичне инфекције) код којих су изазивачи опортунистичке гљивице
које се налазе у спољашњој средини у воденим екосистемима и/или земљишту
(хидрофилне и геофилне гљивице) и микотичне зоонозе (зоонозне гљивичне
инфекције) за које су одговорне зоофилне и антропофилне патогене гљивице и
преносе се са инфицираног домаћина.
У најчешће и најзначајније сапронозе су аспергилоза, мукоромикоза, кандидијаза и
инфекције меланизованим гљивицама, а нешто ређе се јављају кокцидиоидомикоза,
хистоплазмоза, паракокцидиоидомикоза и бластомикоза.
Најзначајније микотичне зоонозе, нарочито са аспекта дерматопатологије су
дерматофиције - инфекције изазване са Microsporum canis, Trichophyton mentagrophytes,
Trichophyton verrucosum, Epidermophyton, Sporothrix sp. и Mallasezia sp.
Група микоза као што су хитридиомикоза водоземаца, узрочник „синдрома белог
носа слепих мишева“ и узрочник „обољења жуте гљивице“ код рептила су од
значаја у домену очувања биодиверзитета јер могу да оболе одређене угрожене
животињске врсте.
У ветеринарској медицини гљивице представљају и значајне контаминенте хране за
животиње које путем својих метаболита (микотоксини) нарушавају здравље
животиња. Поред детекције макроскопских промена карактеристичних за микозе,
за процес дијагностике микоза значајно дијагностичко средство представља
хистопатологија. Специјализоване метода бојења које се поред рутинског бојења
хематоксилином и еозином (HE) користе у дијагностици микоза су Грокот (GMS)
које боји гљивице црном бојом и PAS бојење које боји гљивице ружичасто-црвено
и уоквирује гљивице тако да је омогућена визуелизација њихове морфологије.
Поред хистопатологије, за тачну диференцијацију неких врста гљивица може да се
користи имунохистохемијско испитивање. Гљивице представљају узрочнике који
се све чешће наводе као узрочници обољења која често имају зоонотски карактер.
Ветеринарска патологија има значајну улогу у дијагностици ових обољења на више
нивоа - у домену дерматопатологије, неких генерализованих стања - системских
микоза, као и у оквиру очувања угрожених животињских врста.
AB  - Kingdom Funghi includes as many as 6 million of species of which shapes with
differentiated talus and microscopic forms can be found. 625 species of these have been
reported to cause infection in vertebrates, and beside actual viral and bacterial diseases
mycotic diseases represent the important global diseases which often have zoonotic
character. Mycosis represent diseases caused with fungi. Fungi can infect different tissues
causing superficial and profound pathologic changes, as well as systemic disease. Mycosis
are divided on sapronoses/sapronotic fungal infections caused by opportunistic
environmental fungi that inhabit aquatic ecosystems and/or soil (hydrophilic and geophilic
fungi) rather than a living host, and mycotic zoonoses/zoonotic mycotic infections –
caused by transmissible zoophilic and anthropohilic pathogen fungi which are transmitted
by contagious host.
Most frequent and most importabt sapronoses are aspergillosis, mucoromycosis,
candidiasis, cryptococcosis and infections due to melanised fungi, and less frequent
sapronoses are coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, and
blastomycosis.
Most important fungal zoonoses, especially important in field of dermatopathology are
dermatophytic infections - infections caused by Microsporum canis, Trichophyton
mentagrophytes, Trichophyton verrucosum, Epidermophyton, Sporothrix sp. and Mallasezia sp.
Group of mycosis such as chytridiomycosis of amphibians, then causative agent of “bat
white-nose syndrome” and causative agent of “yellow fungus disease” in reptiles, which
are important in the preservation of biodiversity, because they can jeopardize some
endangered animal species.
In veterinary medicine fungi also represent important food contaminents which have great
impact on animal health causeb with their metabolites (mycotoxin). Besides recognition
of macroscopic changes characteristic for mycoses, histopathology represents an
important diagnostic tool in the diagnostics of these infections. Special stains are used in
diagnosis of fungal infections besides routine haematoxylin and eosin staining (HE).
These staining are Grocott-Gomori's methenamine silver stain (GMS) which stains fungi
black and Periodic acid–Schiff (PAS) which stains fungi pink-red, delineate the fungi so
that their morphology can be visualised. Also, in accurate differentiating of some fungal
species immunohistochemistry can be used. Fungi represent noxes which are more
common stated as cause of diseases which frequent have zoonotic character. Veterinary
pathology plays important role in fungal infection diagnostics weather in domain of
dermatopathology (diagnosis of superficial and subcutaneous mycoses), in recognition of
some general disorders - systemic mycoses, as well as in conservation of endangered
animal species.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici
T1  - Fungal infections – new/old threat? The importance of veterinary pathology in the diagnostic
SP  - 130
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3061
ER  - 
@conference{
author = "Marinković, Darko and Milčić Matić, Natalija and Jelisić, Stefan and Aničić, Milan",
year = "2023",
abstract = "Царство гљива (Funghi) обухвата 6 милиона врста у оквиру којих се срећу облици
којима је развијен талус – гљиве/печурке и микроскопске облике – гљивице и
кваснице од којих 625 врста може да изазове инфекцију кичмењака и поред
тренутно актуелних вирусних и бактеријских обољења представљају све значајнија
обољења глобалног карактера која често имају зоонотски карактер.
Микозе (гљивичне инфекције) представљају обољења изазвана гљивицама.
Гљивице могу да инфицирају различитa ткива изазивајући површинске или дубоке
патолошке промене као и системско обољење. Микозе се деле на сапронозе
(сапронозне гљивичне инфекције) код којих су изазивачи опортунистичке гљивице
које се налазе у спољашњој средини у воденим екосистемима и/или земљишту
(хидрофилне и геофилне гљивице) и микотичне зоонозе (зоонозне гљивичне
инфекције) за које су одговорне зоофилне и антропофилне патогене гљивице и
преносе се са инфицираног домаћина.
У најчешће и најзначајније сапронозе су аспергилоза, мукоромикоза, кандидијаза и
инфекције меланизованим гљивицама, а нешто ређе се јављају кокцидиоидомикоза,
хистоплазмоза, паракокцидиоидомикоза и бластомикоза.
Најзначајније микотичне зоонозе, нарочито са аспекта дерматопатологије су
дерматофиције - инфекције изазване са Microsporum canis, Trichophyton mentagrophytes,
Trichophyton verrucosum, Epidermophyton, Sporothrix sp. и Mallasezia sp.
Група микоза као што су хитридиомикоза водоземаца, узрочник „синдрома белог
носа слепих мишева“ и узрочник „обољења жуте гљивице“ код рептила су од
значаја у домену очувања биодиверзитета јер могу да оболе одређене угрожене
животињске врсте.
У ветеринарској медицини гљивице представљају и значајне контаминенте хране за
животиње које путем својих метаболита (микотоксини) нарушавају здравље
животиња. Поред детекције макроскопских промена карактеристичних за микозе,
за процес дијагностике микоза значајно дијагностичко средство представља
хистопатологија. Специјализоване метода бојења које се поред рутинског бојења
хематоксилином и еозином (HE) користе у дијагностици микоза су Грокот (GMS)
које боји гљивице црном бојом и PAS бојење које боји гљивице ружичасто-црвено
и уоквирује гљивице тако да је омогућена визуелизација њихове морфологије.
Поред хистопатологије, за тачну диференцијацију неких врста гљивица може да се
користи имунохистохемијско испитивање. Гљивице представљају узрочнике који
се све чешће наводе као узрочници обољења која често имају зоонотски карактер.
Ветеринарска патологија има значајну улогу у дијагностици ових обољења на више
нивоа - у домену дерматопатологије, неких генерализованих стања - системских
микоза, као и у оквиру очувања угрожених животињских врста., Kingdom Funghi includes as many as 6 million of species of which shapes with
differentiated talus and microscopic forms can be found. 625 species of these have been
reported to cause infection in vertebrates, and beside actual viral and bacterial diseases
mycotic diseases represent the important global diseases which often have zoonotic
character. Mycosis represent diseases caused with fungi. Fungi can infect different tissues
causing superficial and profound pathologic changes, as well as systemic disease. Mycosis
are divided on sapronoses/sapronotic fungal infections caused by opportunistic
environmental fungi that inhabit aquatic ecosystems and/or soil (hydrophilic and geophilic
fungi) rather than a living host, and mycotic zoonoses/zoonotic mycotic infections –
caused by transmissible zoophilic and anthropohilic pathogen fungi which are transmitted
by contagious host.
Most frequent and most importabt sapronoses are aspergillosis, mucoromycosis,
candidiasis, cryptococcosis and infections due to melanised fungi, and less frequent
sapronoses are coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, and
blastomycosis.
Most important fungal zoonoses, especially important in field of dermatopathology are
dermatophytic infections - infections caused by Microsporum canis, Trichophyton
mentagrophytes, Trichophyton verrucosum, Epidermophyton, Sporothrix sp. and Mallasezia sp.
Group of mycosis such as chytridiomycosis of amphibians, then causative agent of “bat
white-nose syndrome” and causative agent of “yellow fungus disease” in reptiles, which
are important in the preservation of biodiversity, because they can jeopardize some
endangered animal species.
In veterinary medicine fungi also represent important food contaminents which have great
impact on animal health causeb with their metabolites (mycotoxin). Besides recognition
of macroscopic changes characteristic for mycoses, histopathology represents an
important diagnostic tool in the diagnostics of these infections. Special stains are used in
diagnosis of fungal infections besides routine haematoxylin and eosin staining (HE).
These staining are Grocott-Gomori's methenamine silver stain (GMS) which stains fungi
black and Periodic acid–Schiff (PAS) which stains fungi pink-red, delineate the fungi so
that their morphology can be visualised. Also, in accurate differentiating of some fungal
species immunohistochemistry can be used. Fungi represent noxes which are more
common stated as cause of diseases which frequent have zoonotic character. Veterinary
pathology plays important role in fungal infection diagnostics weather in domain of
dermatopathology (diagnosis of superficial and subcutaneous mycoses), in recognition of
some general disorders - systemic mycoses, as well as in conservation of endangered
animal species.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici, Fungal infections – new/old threat? The importance of veterinary pathology in the diagnostic",
pages = "130-131",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3061"
}
Marinković, D., Milčić Matić, N., Jelisić, S.,& Aničić, M.. (2023). Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 130-131.
https://hdl.handle.net/21.15107/rcub_veterinar_3061
Marinković D, Milčić Matić N, Jelisić S, Aničić M. Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:130-131.
https://hdl.handle.net/21.15107/rcub_veterinar_3061 .
Marinković, Darko, Milčić Matić, Natalija, Jelisić, Stefan, Aničić, Milan, "Gljivične infekcije – nova/stara pretnja? – Značaj veterinarske patologije u dijagnostici" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):130-131,
https://hdl.handle.net/21.15107/rcub_veterinar_3061 .

Veterinarska patologija u dijagnostici gljivičnih oboljenja

Marinković, Darko; Milčić-Matić, Natalija; Aničić, Milan

(Banja Luka : Veterinarska komora Republike Srpske, 2022)

TY  - CONF
AU  - Marinković, Darko
AU  - Milčić-Matić, Natalija
AU  - Aničić, Milan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2910
AB  - Гљивичне инфекције/микозе су поремећаји изазвани гљивицама које
могу да инфицирају различитa ткива изазивајући површинске или дубоке
патолошке промене као и системско обољење. Ово су релативно ретка
обољења, али су значајна и за људе и за животиње, јер у појединим
случајевима могу да проузоркују проблеме који су од инетереса по јавно
здравље. Ове инфекције се деле на сапронозне гљивичне инфекције/
сапронозе а изазивачи су опортунистичке гљивице које се налазе у
спољашњој средини у воденим екосистемима и/или земљишту и зоонозне
гљивичне инфекције/гљивичне зоонозе за које су одговорне зоофилне
патогене гљивице и преносе се са инфицираног домаћина.
Најчешће и најзначајније сапронозе су аспергилоза, мукоромикоза,
кандидијаза и инфекције меланизованим гљивицама. Нешто су ређе
кокцидиоидомикоза, хистоплазмоза, пара кокцидиоидомикоза и
бластомикоза.
Најзначајније гљивичне микозе, нарочито са аспекта дерматологије
и дерматопатологије су инфекције изазване са Microsporum canis,
Trichophyton mentagrophytes, Trichophyton verrucosum, Epidermophyton,
Sporothrix sp. и Mallasezia sp.
Група гљивичних инфекција као што су хитридиомикоза водоземаца,
узрочник „синдрома белог носа слепих мишева“ и узрочник „обољења жуте гљивице“ код рептила су од значаја у домену очувања
биодиверзитета јер могу да оболе одређене угрожене животињске
врсте.
Поред препознавања макроскопских промена карактеристичних за
микозе, у процесу дијагностике ових инфекција хистопатологија
представља значајно дијагностичко средство. Неколико
специјализованих метода бојења се користе поред рутинског бојења
хематоксилином и еозином (HE). То су Грокот (GMS) које боји
гљивице црном бојом и PAS бојење које боји гљивице ружичастоцрвено и уоквирује гљивице тако да може да се визуелизује њихова
морфологија. Такође, за тачну диференцијацију неких врста гљивица
може да се користи имунохистохемијско испитивање.
Ветеринарска патологија има значајну улогу у дијагностици гљивичних
обољења било у домену дерматопатологије (дијагностика површинских
и дубоких микоза), било у препознавању неких генерализованих
стања, као што су системске микозе.
AB  - Fungal infections/mycoses are disorders caused by fungus that can invade different tissues, causing superficial and deep pathological changes,
or systemic disease. These are relatively uncommon diseases important
both to animals and human, and in some cases can cause significant
public health problems. These infections are divided on sapronotic fungal
infections/sapronoses – caused by opportunistic environmental fungi
that inhabit aquatic ecosystems and/or soil rather than a living host, and
zoonotic fungal infections/fungal zoonoses – caused by transmissible
zoophilic pathogen fungi which are transmitted by contagious host.
Most frequent and most importabt sapronoses are aspergillosis, mucoromycosis, candidiasis, cryptococcosis and infections due to melanised
fungi. Sapronoses which are less frequent are coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, and blastomycosis.
Most important fungal zoonoses, especially important in field of dermatology and dermatopathology are infections caused by Microsporum
canis, Trichophyton mentagrophytes, Trichophyton verrucosum, Epidermophyton, Sporothrix sp. and Mallasezia sp.
There is group of fungal infections such as chytridiomycosis of amphibians, then causative agent of “bat white-nose syndrome” and causative
agent of “yellow fungus disease” in reptiles, which are important in the preservation of biodiversity, because they can jeopardize some
endangered animal species.
Besides recognition of macroscopic changes characteristic for mycoses, in the diagnostics of these infections histopathology represents
an important diagnostic tool. Few special stains are used in diagnosis
of fungal infections besides routine haematoxylin and eosin staining
(HE). These staining are Grocott-Gomori’s methenamine silver stain
(GMS) which stains fungi black and Periodic acid–Schiff (PAS) which
stains fungi pink-red, delineate the fungi so that their morphology can
be visualised. Also, in accurate differentiating of some fungal species
immunohistochemistry can be used.
Veterinary pathology plays important role in fungal infection diagnostics weather in domain of dermatopathology (diagnosis of superficial
and subcutaneous mycoses), weather in recognition of some general
disorders as systemic mycoses.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 27. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
T1  - Veterinarska patologija u dijagnostici gljivičnih oboljenja
SP  - 193
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2910
ER  - 
@conference{
author = "Marinković, Darko and Milčić-Matić, Natalija and Aničić, Milan",
year = "2022",
abstract = "Гљивичне инфекције/микозе су поремећаји изазвани гљивицама које
могу да инфицирају различитa ткива изазивајући површинске или дубоке
патолошке промене као и системско обољење. Ово су релативно ретка
обољења, али су значајна и за људе и за животиње, јер у појединим
случајевима могу да проузоркују проблеме који су од инетереса по јавно
здравље. Ове инфекције се деле на сапронозне гљивичне инфекције/
сапронозе а изазивачи су опортунистичке гљивице које се налазе у
спољашњој средини у воденим екосистемима и/или земљишту и зоонозне
гљивичне инфекције/гљивичне зоонозе за које су одговорне зоофилне
патогене гљивице и преносе се са инфицираног домаћина.
Најчешће и најзначајније сапронозе су аспергилоза, мукоромикоза,
кандидијаза и инфекције меланизованим гљивицама. Нешто су ређе
кокцидиоидомикоза, хистоплазмоза, пара кокцидиоидомикоза и
бластомикоза.
Најзначајније гљивичне микозе, нарочито са аспекта дерматологије
и дерматопатологије су инфекције изазване са Microsporum canis,
Trichophyton mentagrophytes, Trichophyton verrucosum, Epidermophyton,
Sporothrix sp. и Mallasezia sp.
Група гљивичних инфекција као што су хитридиомикоза водоземаца,
узрочник „синдрома белог носа слепих мишева“ и узрочник „обољења жуте гљивице“ код рептила су од значаја у домену очувања
биодиверзитета јер могу да оболе одређене угрожене животињске
врсте.
Поред препознавања макроскопских промена карактеристичних за
микозе, у процесу дијагностике ових инфекција хистопатологија
представља значајно дијагностичко средство. Неколико
специјализованих метода бојења се користе поред рутинског бојења
хематоксилином и еозином (HE). То су Грокот (GMS) које боји
гљивице црном бојом и PAS бојење које боји гљивице ружичастоцрвено и уоквирује гљивице тако да може да се визуелизује њихова
морфологија. Такође, за тачну диференцијацију неких врста гљивица
може да се користи имунохистохемијско испитивање.
Ветеринарска патологија има значајну улогу у дијагностици гљивичних
обољења било у домену дерматопатологије (дијагностика површинских
и дубоких микоза), било у препознавању неких генерализованих
стања, као што су системске микозе., Fungal infections/mycoses are disorders caused by fungus that can invade different tissues, causing superficial and deep pathological changes,
or systemic disease. These are relatively uncommon diseases important
both to animals and human, and in some cases can cause significant
public health problems. These infections are divided on sapronotic fungal
infections/sapronoses – caused by opportunistic environmental fungi
that inhabit aquatic ecosystems and/or soil rather than a living host, and
zoonotic fungal infections/fungal zoonoses – caused by transmissible
zoophilic pathogen fungi which are transmitted by contagious host.
Most frequent and most importabt sapronoses are aspergillosis, mucoromycosis, candidiasis, cryptococcosis and infections due to melanised
fungi. Sapronoses which are less frequent are coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, and blastomycosis.
Most important fungal zoonoses, especially important in field of dermatology and dermatopathology are infections caused by Microsporum
canis, Trichophyton mentagrophytes, Trichophyton verrucosum, Epidermophyton, Sporothrix sp. and Mallasezia sp.
There is group of fungal infections such as chytridiomycosis of amphibians, then causative agent of “bat white-nose syndrome” and causative
agent of “yellow fungus disease” in reptiles, which are important in the preservation of biodiversity, because they can jeopardize some
endangered animal species.
Besides recognition of macroscopic changes characteristic for mycoses, in the diagnostics of these infections histopathology represents
an important diagnostic tool. Few special stains are used in diagnosis
of fungal infections besides routine haematoxylin and eosin staining
(HE). These staining are Grocott-Gomori’s methenamine silver stain
(GMS) which stains fungi black and Periodic acid–Schiff (PAS) which
stains fungi pink-red, delineate the fungi so that their morphology can
be visualised. Also, in accurate differentiating of some fungal species
immunohistochemistry can be used.
Veterinary pathology plays important role in fungal infection diagnostics weather in domain of dermatopathology (diagnosis of superficial
and subcutaneous mycoses), weather in recognition of some general
disorders as systemic mycoses.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "27. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022",
title = "Veterinarska patologija u dijagnostici gljivičnih oboljenja",
pages = "193-196",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2910"
}
Marinković, D., Milčić-Matić, N.,& Aničić, M.. (2022). Veterinarska patologija u dijagnostici gljivičnih oboljenja. in 27. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
Banja Luka : Veterinarska komora Republike Srpske., 193-196.
https://hdl.handle.net/21.15107/rcub_veterinar_2910
Marinković D, Milčić-Matić N, Aničić M. Veterinarska patologija u dijagnostici gljivičnih oboljenja. in 27. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022. 2022;:193-196.
https://hdl.handle.net/21.15107/rcub_veterinar_2910 .
Marinković, Darko, Milčić-Matić, Natalija, Aničić, Milan, "Veterinarska patologija u dijagnostici gljivičnih oboljenja" in 27. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022 (2022):193-196,
https://hdl.handle.net/21.15107/rcub_veterinar_2910 .

Mastocitomi – da li je hirurgija jedino rešenje?

Milčić Matić, Natalija; Vučićević, Ivana

(Beograd : Fakultet veterinarske medicine, 2022)

TY  - CONF
AU  - Milčić Matić, Natalija
AU  - Vučićević, Ivana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2894
PB  - Beograd : Fakultet veterinarske medicine
C3  - Clinica veterinaria zbornik predavanja Dvadeset trećeg regionalnog savetovanja iz kliničke patologije i terapije malih životinja, Fruška gora, 09 - 11. jun, 2022
T1  - Mastocitomi – da li je hirurgija jedino rešenje?
SP  - 12
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2894
ER  - 
@conference{
author = "Milčić Matić, Natalija and Vučićević, Ivana",
year = "2022",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Clinica veterinaria zbornik predavanja Dvadeset trećeg regionalnog savetovanja iz kliničke patologije i terapije malih životinja, Fruška gora, 09 - 11. jun, 2022",
title = "Mastocitomi – da li je hirurgija jedino rešenje?",
pages = "12-14",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2894"
}
Milčić Matić, N.,& Vučićević, I.. (2022). Mastocitomi – da li je hirurgija jedino rešenje?. in Clinica veterinaria zbornik predavanja Dvadeset trećeg regionalnog savetovanja iz kliničke patologije i terapije malih životinja, Fruška gora, 09 - 11. jun, 2022
Beograd : Fakultet veterinarske medicine., 12-14.
https://hdl.handle.net/21.15107/rcub_veterinar_2894
Milčić Matić N, Vučićević I. Mastocitomi – da li je hirurgija jedino rešenje?. in Clinica veterinaria zbornik predavanja Dvadeset trećeg regionalnog savetovanja iz kliničke patologije i terapije malih životinja, Fruška gora, 09 - 11. jun, 2022. 2022;:12-14.
https://hdl.handle.net/21.15107/rcub_veterinar_2894 .
Milčić Matić, Natalija, Vučićević, Ivana, "Mastocitomi – da li je hirurgija jedino rešenje?" in Clinica veterinaria zbornik predavanja Dvadeset trećeg regionalnog savetovanja iz kliničke patologije i terapije malih životinja, Fruška gora, 09 - 11. jun, 2022 (2022):12-14,
https://hdl.handle.net/21.15107/rcub_veterinar_2894 .

Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease

Kecova, Helga; Milčić-Matić, Natalija; Lazić, Tatjana; Grozdanić, Sinisa

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2021)

TY  - JOUR
AU  - Kecova, Helga
AU  - Milčić-Matić, Natalija
AU  - Lazić, Tatjana
AU  - Grozdanić, Sinisa
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2063
AB  - A four-year-old intact male Labrador retriever was presented with acute onset of ulcerative lesions around both eyes, on the mucocutaneous junctions around the muzzle and nares, and on the lateral aspects of paw pads. All lesions were symmetrical and well demarcated. The rest of ocular and systemic examination was unremarkable. The onset of lesions was acute and coincided with an episode of intense hunting in switchgrass. Similar lesions were present in another dog used in the same hunting trip. The clinical presentation was suggestive of a possible immune-mediated skin disease. However, the patient responded to systemic antibiotic treatment with full recovery without recurrence upon cessation of therapy. Traumatic origin should be included into differential diagnosis of mucocutaneous ulcerative lesions presenting as a possible immune-mediated skin disease.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease
VL  - 71
IS  - 1
SP  - 120
EP  - 130
DO  - 10.2478/acve-2021-0010
ER  - 
@article{
author = "Kecova, Helga and Milčić-Matić, Natalija and Lazić, Tatjana and Grozdanić, Sinisa",
year = "2021",
abstract = "A four-year-old intact male Labrador retriever was presented with acute onset of ulcerative lesions around both eyes, on the mucocutaneous junctions around the muzzle and nares, and on the lateral aspects of paw pads. All lesions were symmetrical and well demarcated. The rest of ocular and systemic examination was unremarkable. The onset of lesions was acute and coincided with an episode of intense hunting in switchgrass. Similar lesions were present in another dog used in the same hunting trip. The clinical presentation was suggestive of a possible immune-mediated skin disease. However, the patient responded to systemic antibiotic treatment with full recovery without recurrence upon cessation of therapy. Traumatic origin should be included into differential diagnosis of mucocutaneous ulcerative lesions presenting as a possible immune-mediated skin disease.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease",
volume = "71",
number = "1",
pages = "120-130",
doi = "10.2478/acve-2021-0010"
}
Kecova, H., Milčić-Matić, N., Lazić, T.,& Grozdanić, S.. (2021). Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 120-130.
https://doi.org/10.2478/acve-2021-0010
Kecova H, Milčić-Matić N, Lazić T, Grozdanić S. Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease. in Acta Veterinaria-Beograd. 2021;71(1):120-130.
doi:10.2478/acve-2021-0010 .
Kecova, Helga, Milčić-Matić, Natalija, Lazić, Tatjana, Grozdanić, Sinisa, "Traumatic blepharitis including cutanous changes with a clinical appearance of immune-mediated disease" in Acta Veterinaria-Beograd, 71, no. 1 (2021):120-130,
https://doi.org/10.2478/acve-2021-0010 . .
1
1

Focal pruritus and alopecia in lumbar area in guinea pigs

Vučićević, Miloš; Dosenović, Milan; Mitrović, Marko Jumake; Simović, Nikola; Milčić-Matić, Natalija; Aničić, Milan

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2021)

TY  - JOUR
AU  - Vučićević, Miloš
AU  - Dosenović, Milan
AU  - Mitrović, Marko Jumake
AU  - Simović, Nikola
AU  - Milčić-Matić, Natalija
AU  - Aničić, Milan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2074
AB  - From June to December 2017, four male guinea pigs (3-4 years old) with different owners were brought to the Clinic for Small Animals at the Faculty of Veterinary Medicine, University of Belgrade. All of them presented with an identical symptom – severe pruritus on the dorsal rump. The animals were scratching so intensely that they appeared to be having a seizure. Also, all owners noticed that their animals had become aggressive since the onset of symptoms. One of the animals had selfmutilation trauma. One of the guinea pigs also had a hairless area behind its ears. Also, one animal lived in a pair with another guinea pig that did not have any skin changes.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Focal pruritus and alopecia in lumbar area in guinea pigs
VL  - 75
IS  - 1
SP  - 92
EP  - 98
DO  - 10.2298/VETGL180503002V
ER  - 
@article{
author = "Vučićević, Miloš and Dosenović, Milan and Mitrović, Marko Jumake and Simović, Nikola and Milčić-Matić, Natalija and Aničić, Milan",
year = "2021",
abstract = "From June to December 2017, four male guinea pigs (3-4 years old) with different owners were brought to the Clinic for Small Animals at the Faculty of Veterinary Medicine, University of Belgrade. All of them presented with an identical symptom – severe pruritus on the dorsal rump. The animals were scratching so intensely that they appeared to be having a seizure. Also, all owners noticed that their animals had become aggressive since the onset of symptoms. One of the animals had selfmutilation trauma. One of the guinea pigs also had a hairless area behind its ears. Also, one animal lived in a pair with another guinea pig that did not have any skin changes.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Focal pruritus and alopecia in lumbar area in guinea pigs",
volume = "75",
number = "1",
pages = "92-98",
doi = "10.2298/VETGL180503002V"
}
Vučićević, M., Dosenović, M., Mitrović, M. J., Simović, N., Milčić-Matić, N.,& Aničić, M.. (2021). Focal pruritus and alopecia in lumbar area in guinea pigs. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 92-98.
https://doi.org/10.2298/VETGL180503002V
Vučićević M, Dosenović M, Mitrović MJ, Simović N, Milčić-Matić N, Aničić M. Focal pruritus and alopecia in lumbar area in guinea pigs. in Veterinarski Glasnik. 2021;75(1):92-98.
doi:10.2298/VETGL180503002V .
Vučićević, Miloš, Dosenović, Milan, Mitrović, Marko Jumake, Simović, Nikola, Milčić-Matić, Natalija, Aničić, Milan, "Focal pruritus and alopecia in lumbar area in guinea pigs" in Veterinarski Glasnik, 75, no. 1 (2021):92-98,
https://doi.org/10.2298/VETGL180503002V . .

Kušingov sindrom: onkološko ili endokrino oboljenje?

Milčić-Matić, Natalija

(Beograd : Srpsko veterinarsko društvo, 2021)

TY  - CONF
AU  - Milčić-Matić, Natalija
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3169
AB  - Kušingov sindrom (hiperadrenokorticizam, hiperkortizolizam) je relativno često
oboljenje pasa sa prevalencom od oko 0,28 procenata. Javlja se kao jatrogen (dugotrajna
upotreba kortikosteroida), a spontano nastali može biti pituitarno zavisan, kada su
na hipofizi prisutni mikro ili makroadenomi ili adrenalno zavisan koji nastaje kao posledica
tumora na nadbubrežnoj žlezdi. Do bolesti dolazi ili usled hipersekrecije adrenokortikalnog
hormona (ACTH) ili pojačanog lučenja kortikosteroida, a klinički simptomi,
kao i promene biohemijskih parametara nastaju kao posledica dugotrajne visoke
koncentracije kortizola u plazmi. Dijagnoza ovog oboljenja se postavlja na osnovu kliničkih
simptoma, istorije bolesti, hematoloških i biohemijskih parametara i hormonskih
testova, a tumor se može vizualizovati ultrazvučnim pregledom ili snimanjem na
skeneru ili magnetnoj rezonanci. Terapija zavisi od lokalizacije tumora, tako da je kod
adrenalno zavisnog hiperadrenokorticizma najbolje hiruški ukloniti tumor, ukoliko je
to moguće. Pituitarno zavisni hiperadrenokorticizm, kao i kada nije moguće hiruško
uklanjanje tumora na nadbubrežnoj žlezdi, tretira se lekovima kao što su trilostan i mitotan.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
T1  - Kušingov sindrom: onkološko ili endokrino oboljenje?
SP  - 303
EP  - 308
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3169
ER  - 
@conference{
author = "Milčić-Matić, Natalija",
year = "2021",
abstract = "Kušingov sindrom (hiperadrenokorticizam, hiperkortizolizam) je relativno često
oboljenje pasa sa prevalencom od oko 0,28 procenata. Javlja se kao jatrogen (dugotrajna
upotreba kortikosteroida), a spontano nastali može biti pituitarno zavisan, kada su
na hipofizi prisutni mikro ili makroadenomi ili adrenalno zavisan koji nastaje kao posledica
tumora na nadbubrežnoj žlezdi. Do bolesti dolazi ili usled hipersekrecije adrenokortikalnog
hormona (ACTH) ili pojačanog lučenja kortikosteroida, a klinički simptomi,
kao i promene biohemijskih parametara nastaju kao posledica dugotrajne visoke
koncentracije kortizola u plazmi. Dijagnoza ovog oboljenja se postavlja na osnovu kliničkih
simptoma, istorije bolesti, hematoloških i biohemijskih parametara i hormonskih
testova, a tumor se može vizualizovati ultrazvučnim pregledom ili snimanjem na
skeneru ili magnetnoj rezonanci. Terapija zavisi od lokalizacije tumora, tako da je kod
adrenalno zavisnog hiperadrenokorticizma najbolje hiruški ukloniti tumor, ukoliko je
to moguće. Pituitarno zavisni hiperadrenokorticizm, kao i kada nije moguće hiruško
uklanjanje tumora na nadbubrežnoj žlezdi, tretira se lekovima kao što su trilostan i mitotan.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021",
title = "Kušingov sindrom: onkološko ili endokrino oboljenje?",
pages = "303-308",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3169"
}
Milčić-Matić, N.. (2021). Kušingov sindrom: onkološko ili endokrino oboljenje?. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
Beograd : Srpsko veterinarsko društvo., 303-308.
https://hdl.handle.net/21.15107/rcub_veterinar_3169
Milčić-Matić N. Kušingov sindrom: onkološko ili endokrino oboljenje?. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021. 2021;:303-308.
https://hdl.handle.net/21.15107/rcub_veterinar_3169 .
Milčić-Matić, Natalija, "Kušingov sindrom: onkološko ili endokrino oboljenje?" in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021 (2021):303-308,
https://hdl.handle.net/21.15107/rcub_veterinar_3169 .

Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation

Đurić, Miloš; Milčić-Matić, Natalija; Davitkov, Darko; Glavinić, Uroš; Davitkov, Dajana; Vejnović, Branislav; Stanimirović, Zoran

(BMC, London, 2019)

TY  - JOUR
AU  - Đurić, Miloš
AU  - Milčić-Matić, Natalija
AU  - Davitkov, Darko
AU  - Glavinić, Uroš
AU  - Davitkov, Dajana
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1742
AB  - BackgroundCanine generalized demodicosis is a common parasitic disease caused by the proliferation of Demodex mites. The introduction of isoxazoline class treatments in veterinary dermatology has resulted in apparently effective treatment of generalized demodicosis. The objective of this study was to evaluate the effectiveness of fluralaner for the treatment of canine generalized demodicosis using real-time PCR for the detection and quantification of Demodex DNA.MethodsTwenty privately owned dogs with clinical symptoms of generalized demodicosis and deep skin scrapings positive for Demodex canis mites were enrolled in the study. Following diagnosis (day 0) each dog was treated with fluralaner at the recommended commercial dose for tick and flea treatment (25-56 mg/kg) based on body weight. Clinical and mite count assessments, and hair sampling for molecular analyses were performed on days 0, 28, 56, 84 and 112. Demodex DNA was detected and quantified using real-time PCR.ResultsA single oral dose of fluralaner reduced Demodex mite counts in skin scrapings by an average of 98.9% in all dogs by day 28. No mites were recovered from skin scrapings from any treated dog by day 56, at which time the dog was considered to be clinically cured, with total hair regrowth. There were significant differences among examined dogs in qPCR cycle threshold (Ct) values on days 0, 28, 56, 84 and 112. Demodex DNA levels decreased (increasing Ct values) throughout the study. Mite DNA was present on day 112, possibly from dead mites, at values significantly lower than in samples taken on days 0, 28 and 56. Based on qPCR testing of diluted samples, the Demodex mite population was reduced by approximately 1000-fold on day 112.ConclusionsOral administration of fluralaner at the recommended dose to dogs with generalized demodicosis is highly effective for reducing Demodex mite populations and resolving clinical signs of generalized demodicosis. The presence of mite DNA may indicate that treatment did not kill all Demodex mites.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation
VL  - 12
SP  - 270
DO  - 10.1186/s13071-019-3521-9
ER  - 
@article{
author = "Đurić, Miloš and Milčić-Matić, Natalija and Davitkov, Darko and Glavinić, Uroš and Davitkov, Dajana and Vejnović, Branislav and Stanimirović, Zoran",
year = "2019",
abstract = "BackgroundCanine generalized demodicosis is a common parasitic disease caused by the proliferation of Demodex mites. The introduction of isoxazoline class treatments in veterinary dermatology has resulted in apparently effective treatment of generalized demodicosis. The objective of this study was to evaluate the effectiveness of fluralaner for the treatment of canine generalized demodicosis using real-time PCR for the detection and quantification of Demodex DNA.MethodsTwenty privately owned dogs with clinical symptoms of generalized demodicosis and deep skin scrapings positive for Demodex canis mites were enrolled in the study. Following diagnosis (day 0) each dog was treated with fluralaner at the recommended commercial dose for tick and flea treatment (25-56 mg/kg) based on body weight. Clinical and mite count assessments, and hair sampling for molecular analyses were performed on days 0, 28, 56, 84 and 112. Demodex DNA was detected and quantified using real-time PCR.ResultsA single oral dose of fluralaner reduced Demodex mite counts in skin scrapings by an average of 98.9% in all dogs by day 28. No mites were recovered from skin scrapings from any treated dog by day 56, at which time the dog was considered to be clinically cured, with total hair regrowth. There were significant differences among examined dogs in qPCR cycle threshold (Ct) values on days 0, 28, 56, 84 and 112. Demodex DNA levels decreased (increasing Ct values) throughout the study. Mite DNA was present on day 112, possibly from dead mites, at values significantly lower than in samples taken on days 0, 28 and 56. Based on qPCR testing of diluted samples, the Demodex mite population was reduced by approximately 1000-fold on day 112.ConclusionsOral administration of fluralaner at the recommended dose to dogs with generalized demodicosis is highly effective for reducing Demodex mite populations and resolving clinical signs of generalized demodicosis. The presence of mite DNA may indicate that treatment did not kill all Demodex mites.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation",
volume = "12",
pages = "270",
doi = "10.1186/s13071-019-3521-9"
}
Đurić, M., Milčić-Matić, N., Davitkov, D., Glavinić, U., Davitkov, D., Vejnović, B.,& Stanimirović, Z.. (2019). Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation. in Parasites & Vectors
BMC, London., 12, 270.
https://doi.org/10.1186/s13071-019-3521-9
Đurić M, Milčić-Matić N, Davitkov D, Glavinić U, Davitkov D, Vejnović B, Stanimirović Z. Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation. in Parasites & Vectors. 2019;12:270.
doi:10.1186/s13071-019-3521-9 .
Đurić, Miloš, Milčić-Matić, Natalija, Davitkov, Darko, Glavinić, Uroš, Davitkov, Dajana, Vejnović, Branislav, Stanimirović, Zoran, "Efficacy of oral fluralaner for the treatment of canine generalized demodicosis: a molecular-level confirmation" in Parasites & Vectors, 12 (2019):270,
https://doi.org/10.1186/s13071-019-3521-9 . .
3
8
3
6

Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis

Marinković, Darko; Milčić-Matić, Natalija; Jovanović, Milan; Vučićević, Ivana; Nešić, Slađan; Aničić, Milan; Aleksić-Kovačević, Sanja

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2015)

TY  - JOUR
AU  - Marinković, Darko
AU  - Milčić-Matić, Natalija
AU  - Jovanović, Milan
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1199
AB  - Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis
VL  - 65
IS  - 2
SP  - 226
EP  - 237
DO  - 10.1515/acve-2015-0019
ER  - 
@article{
author = "Marinković, Darko and Milčić-Matić, Natalija and Jovanović, Milan and Vučićević, Ivana and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2015",
abstract = "Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis",
volume = "65",
number = "2",
pages = "226-237",
doi = "10.1515/acve-2015-0019"
}
Marinković, D., Milčić-Matić, N., Jovanović, M., Vučićević, I., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2015). Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(2), 226-237.
https://doi.org/10.1515/acve-2015-0019
Marinković D, Milčić-Matić N, Jovanović M, Vučićević I, Nešić S, Aničić M, Aleksić-Kovačević S. Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd. 2015;65(2):226-237.
doi:10.1515/acve-2015-0019 .
Marinković, Darko, Milčić-Matić, Natalija, Jovanović, Milan, Vučićević, Ivana, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis" in Acta Veterinaria-Beograd, 65, no. 2 (2015):226-237,
https://doi.org/10.1515/acve-2015-0019 . .
2
2
2

Diversity of allergens contained in dog saliva REPLY

van Hage, M.; Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, Natalija; Bergman, T.; Gronlund, H.

(Wiley, Hoboken, 2013)

TY  - JOUR
AU  - van Hage, M.
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/986
PB  - Wiley, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Diversity of allergens contained in dog saliva REPLY
VL  - 68
IS  - 11
SP  - 1485
EP  - 1486
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_986
ER  - 
@article{
author = "van Hage, M. and Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H.",
year = "2013",
publisher = "Wiley, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Diversity of allergens contained in dog saliva REPLY",
volume = "68",
number = "11",
pages = "1485-1486",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_986"
}
van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Gronlund, H.. (2013). Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley, Hoboken., 68(11), 1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986
van Hage M, Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H. Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(11):1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986 .
van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., "Diversity of allergens contained in dog saliva REPLY" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 11 (2013):1485-1486,
https://hdl.handle.net/21.15107/rcub_veterinar_986 .

Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis

Ognjenović, Jana; Milčić-Matić, Natalija; Smiljanić, Katarina; Vucković, Olga; Burazer, Lidija; Popović, Nikola; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Elsevier Science Bv, Amsterdam, 2013)

TY  - JOUR
AU  - Ognjenović, Jana
AU  - Milčić-Matić, Natalija
AU  - Smiljanić, Katarina
AU  - Vucković, Olga
AU  - Burazer, Lidija
AU  - Popović, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/998
AB  - Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dogs sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Immunology and Immunopathology
T1  - Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis
VL  - 155
IS  - 1-2
SP  - 38
EP  - 47
DO  - 10.1016/j.vetimm.2013.06.005
ER  - 
@article{
author = "Ognjenović, Jana and Milčić-Matić, Natalija and Smiljanić, Katarina and Vucković, Olga and Burazer, Lidija and Popović, Nikola and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2013",
abstract = "Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dogs sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Immunology and Immunopathology",
title = "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis",
volume = "155",
number = "1-2",
pages = "38-47",
doi = "10.1016/j.vetimm.2013.06.005"
}
Ognjenović, J., Milčić-Matić, N., Smiljanić, K., Vucković, O., Burazer, L., Popović, N., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2013). Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology
Elsevier Science Bv, Amsterdam., 155(1-2), 38-47.
https://doi.org/10.1016/j.vetimm.2013.06.005
Ognjenović J, Milčić-Matić N, Smiljanić K, Vucković O, Burazer L, Popović N, Stanić-Vučinić D, Ćirković-Veličković T. Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology. 2013;155(1-2):38-47.
doi:10.1016/j.vetimm.2013.06.005 .
Ognjenović, Jana, Milčić-Matić, Natalija, Smiljanić, Katarina, Vucković, Olga, Burazer, Lidija, Popović, Nikola, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis" in Veterinary Immunology and Immunopathology, 155, no. 1-2 (2013):38-47,
https://doi.org/10.1016/j.vetimm.2013.06.005 . .
14
9
11

Dog saliva - an important source of dog allergens

Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, Natalija; Bergman, T.; Gronlund, H.; van Hage, M.

(Wiley-Blackwell, Hoboken, 2013)

TY  - JOUR
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1026
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - an important source of dog allergens
VL  - 68
IS  - 5
SP  - 585
EP  - 592
DO  - 10.1111/all.12130
ER  - 
@article{
author = "Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H. and van Hage, M.",
year = "2013",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
number = "5",
pages = "585-592",
doi = "10.1111/all.12130"
}
Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 68(5), 585-592.
https://doi.org/10.1111/all.12130
Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(5):585-592.
doi:10.1111/all.12130 .
Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., van Hage, M., "Dog saliva - an important source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 5 (2013):585-592,
https://doi.org/10.1111/all.12130 . .
209
38
25
31

Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen

Milčić-Matić, Natalija; Ognjenović, Jana; Burazer, Lidija; Blagojević, G.; Popović, N.; Lazarević, Miodrag; Stanić-Vučinić, Dragana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2013)

TY  - JOUR
AU  - Milčić-Matić, Natalija
AU  - Ognjenović, Jana
AU  - Burazer, Lidija
AU  - Blagojević, G.
AU  - Popović, N.
AU  - Lazarević, Miodrag
AU  - Stanić-Vučinić, Dragana
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1062
AB  - Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses.
AB  - Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen
T1  - Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia
VL  - 63
IS  - 4
SP  - 437
EP  - 451
DO  - 10.2298/AVB1304437M
ER  - 
@article{
author = "Milčić-Matić, Natalija and Ognjenović, Jana and Burazer, Lidija and Blagojević, G. and Popović, N. and Lazarević, Miodrag and Stanić-Vučinić, Dragana",
year = "2013",
abstract = "Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses., Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen, Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia",
volume = "63",
number = "4",
pages = "437-451",
doi = "10.2298/AVB1304437M"
}
Milčić-Matić, N., Ognjenović, J., Burazer, L., Blagojević, G., Popović, N., Lazarević, M.,& Stanić-Vučinić, D.. (2013). Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 437-451.
https://doi.org/10.2298/AVB1304437M
Milčić-Matić N, Ognjenović J, Burazer L, Blagojević G, Popović N, Lazarević M, Stanić-Vučinić D. Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen. in Acta Veterinaria-Beograd. 2013;63(4):437-451.
doi:10.2298/AVB1304437M .
Milčić-Matić, Natalija, Ognjenović, Jana, Burazer, Lidija, Blagojević, G., Popović, N., Lazarević, Miodrag, Stanić-Vučinić, Dragana, "Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen" in Acta Veterinaria-Beograd, 63, no. 4 (2013):437-451,
https://doi.org/10.2298/AVB1304437M . .
1
1

Diversity of allergens contained in dog saliva

Codina, R.; Van Hage, M.; Polović, N.; Wadén, K.; Binnmyr, J.; Hamsten, C.; Grönneberg, R.; Palmberg, C.; Milčić-Matić, Natalija; Bergman, T.; Grönlund, H.

(Blackwell Publishing Ltd, 2013)

TY  - JOUR
AU  - Codina, R.
AU  - Van Hage, M.
AU  - Polović, N.
AU  - Wadén, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Grönneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Grönlund, H.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1070
PB  - Blackwell Publishing Ltd
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Diversity of allergens contained in dog saliva
VL  - 68
IS  - 11
SP  - 1484
EP  - 1486
DO  - 10.1111/all.12264
ER  - 
@article{
author = "Codina, R. and Van Hage, M. and Polović, N. and Wadén, K. and Binnmyr, J. and Hamsten, C. and Grönneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Grönlund, H.",
year = "2013",
publisher = "Blackwell Publishing Ltd",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Diversity of allergens contained in dog saliva",
volume = "68",
number = "11",
pages = "1484-1486",
doi = "10.1111/all.12264"
}
Codina, R., Van Hage, M., Polović, N., Wadén, K., Binnmyr, J., Hamsten, C., Grönneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Grönlund, H.. (2013). Diversity of allergens contained in dog saliva. in Allergy: European Journal of Allergy and Clinical Immunology
Blackwell Publishing Ltd., 68(11), 1484-1486.
https://doi.org/10.1111/all.12264
Codina R, Van Hage M, Polović N, Wadén K, Binnmyr J, Hamsten C, Grönneberg R, Palmberg C, Milčić-Matić N, Bergman T, Grönlund H. Diversity of allergens contained in dog saliva. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(11):1484-1486.
doi:10.1111/all.12264 .
Codina, R., Van Hage, M., Polović, N., Wadén, K., Binnmyr, J., Hamsten, C., Grönneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Grönlund, H., "Diversity of allergens contained in dog saliva" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 11 (2013):1484-1486,
https://doi.org/10.1111/all.12264 . .

The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis

Milčić-Matić, Natalija; Popović, N.; Lazarević, Miodrag; Medenica, Ljiljana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2010)

TY  - JOUR
AU  - Milčić-Matić, Natalija
AU  - Popović, N.
AU  - Lazarević, Miodrag
AU  - Medenica, Ljiljana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/669
AB  - Pollens from Ambrosia species have a strong allergenic potential and they represent a complex mixture of at least 52 different antigens. This weed is widespread in Serbia and has a long pollination period (appr. 100 days). Pollens are present in very high concentrations during the pollination season (up to 300 pg/m3 of air). Fifty dogs with a clinical diagnosis of canine atopic dermatitis (CAD) and positive intradermal skin test (IDST) reactions to other environmental allergens were included in this study along with 30 dogs without clinical signs of CAD. Both groups were tested intradermally with Ambrosia artemisiifolia extract in concentration of 1000 PNU/mL (provided by ''Institute of virology, vaccines and sera - Torlak''). We have found that Ambrosia extract was second most common allergen to yield positive intradermal test reactions just after house dust mites. The positive reaction of IDST was observed in 66% of the atopic dogs and in 10% of clinically healthy dogs. Dogs that had a positive reaction on intradermal skin test to Ambrosia pollens, mostly showed secondary skin lesions on the pads and perioral skin. In 88% of cases, Malassezia pachydermatis have been isolated from the skin in an increased number and 64% of dogs had otitis externa. These results indicate, that allergens from Ambrosia artemisifolia are of great importance in the etiopathogenesis of atopic dermatitis in dogs originating from the Belgrade region.
AB  - Polen ambrozije ima jaka alergijska svojstva i sastoji se od kompleksne mešavine od do sada poznatih 52 antigena. Ova korovska biljka je znatno rasprostranjena u Srbiji i ima dugačak period polinacije od prosečno 100 dana. Polen je prisutan u izrazito visokim koncentracijama tokom avgusta i septembra ide i do 300 polenovih zrna u m3 vazduha. Ovim ispitivanjem obuhvaćeno je 50 pasa kod kojih je dijagnostikovan atopijski dermatitis na osnovu kliničkih simptoma i pozitivne reakcije na intradermalnom testu, kao i 30 pasa bez klinički manifestnih simptoma atopijskog dermatitisa. Kod obe grupe pasa uradjeno je intradermalno testiranje standardnim rastvorima proizvođača Instituta za imunologiju i virusologiju Torlak, kao i vodenim rastvorom alergena ambrozije u koncentraciji 1000 PNU/ml. U našem ispitivanju polen ambrozije predstavlja drugi alergen po učestalosti pozitivne reakcije na intradermalnom testu kod ispitivanih pasa, odmah posle kućnih grinja. Pozitivna reakcija zabeležena je kod 66% pasa iz grupe atopičara i kod 10% klinički zdravih pasa. Kod pasa koji su pokazali pozitivnu reakciju na intradermalnom testu na polen ambrozije, sekundarne lezije kože su se najčešće javljale na šapama i oko usana. Kod 88% ovih pasa na koži je u povećanom broju dijagnostikovana Malassezia pachydermatis, a kod 64% jedinki je registrovan otitis externa različite etiologije. Na osnovu dobijenih rezultata se može zaključiti da je alergen biljke Ambrosia artemisiifolia veoma značajan u indukciji atopijskog dermatitisa kod populacije pasa sa šireg područja Beograda.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis
T1  - Značaj alergena biljke Ambrosia artemisiifolia u etiopatogenezi atopijskog dermatitisa pasa
VL  - 60
IS  - 2-3
SP  - 183
EP  - 196
DO  - 10.2298/AVB1003183M
ER  - 
@article{
author = "Milčić-Matić, Natalija and Popović, N. and Lazarević, Miodrag and Medenica, Ljiljana",
year = "2010",
abstract = "Pollens from Ambrosia species have a strong allergenic potential and they represent a complex mixture of at least 52 different antigens. This weed is widespread in Serbia and has a long pollination period (appr. 100 days). Pollens are present in very high concentrations during the pollination season (up to 300 pg/m3 of air). Fifty dogs with a clinical diagnosis of canine atopic dermatitis (CAD) and positive intradermal skin test (IDST) reactions to other environmental allergens were included in this study along with 30 dogs without clinical signs of CAD. Both groups were tested intradermally with Ambrosia artemisiifolia extract in concentration of 1000 PNU/mL (provided by ''Institute of virology, vaccines and sera - Torlak''). We have found that Ambrosia extract was second most common allergen to yield positive intradermal test reactions just after house dust mites. The positive reaction of IDST was observed in 66% of the atopic dogs and in 10% of clinically healthy dogs. Dogs that had a positive reaction on intradermal skin test to Ambrosia pollens, mostly showed secondary skin lesions on the pads and perioral skin. In 88% of cases, Malassezia pachydermatis have been isolated from the skin in an increased number and 64% of dogs had otitis externa. These results indicate, that allergens from Ambrosia artemisifolia are of great importance in the etiopathogenesis of atopic dermatitis in dogs originating from the Belgrade region., Polen ambrozije ima jaka alergijska svojstva i sastoji se od kompleksne mešavine od do sada poznatih 52 antigena. Ova korovska biljka je znatno rasprostranjena u Srbiji i ima dugačak period polinacije od prosečno 100 dana. Polen je prisutan u izrazito visokim koncentracijama tokom avgusta i septembra ide i do 300 polenovih zrna u m3 vazduha. Ovim ispitivanjem obuhvaćeno je 50 pasa kod kojih je dijagnostikovan atopijski dermatitis na osnovu kliničkih simptoma i pozitivne reakcije na intradermalnom testu, kao i 30 pasa bez klinički manifestnih simptoma atopijskog dermatitisa. Kod obe grupe pasa uradjeno je intradermalno testiranje standardnim rastvorima proizvođača Instituta za imunologiju i virusologiju Torlak, kao i vodenim rastvorom alergena ambrozije u koncentraciji 1000 PNU/ml. U našem ispitivanju polen ambrozije predstavlja drugi alergen po učestalosti pozitivne reakcije na intradermalnom testu kod ispitivanih pasa, odmah posle kućnih grinja. Pozitivna reakcija zabeležena je kod 66% pasa iz grupe atopičara i kod 10% klinički zdravih pasa. Kod pasa koji su pokazali pozitivnu reakciju na intradermalnom testu na polen ambrozije, sekundarne lezije kože su se najčešće javljale na šapama i oko usana. Kod 88% ovih pasa na koži je u povećanom broju dijagnostikovana Malassezia pachydermatis, a kod 64% jedinki je registrovan otitis externa različite etiologije. Na osnovu dobijenih rezultata se može zaključiti da je alergen biljke Ambrosia artemisiifolia veoma značajan u indukciji atopijskog dermatitisa kod populacije pasa sa šireg područja Beograda.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis, Značaj alergena biljke Ambrosia artemisiifolia u etiopatogenezi atopijskog dermatitisa pasa",
volume = "60",
number = "2-3",
pages = "183-196",
doi = "10.2298/AVB1003183M"
}
Milčić-Matić, N., Popović, N., Lazarević, M.,& Medenica, L.. (2010). The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 183-196.
https://doi.org/10.2298/AVB1003183M
Milčić-Matić N, Popović N, Lazarević M, Medenica L. The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis. in Acta Veterinaria-Beograd. 2010;60(2-3):183-196.
doi:10.2298/AVB1003183M .
Milčić-Matić, Natalija, Popović, N., Lazarević, Miodrag, Medenica, Ljiljana, "The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):183-196,
https://doi.org/10.2298/AVB1003183M . .
2
3
3

The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens

Mihajlović, L.; Cirkovic-Velicković, T.; Jadranin, Milka; Burazer, Lidija; Milčić-Matić, Natalija

(Wiley-Blackwell, Hoboken, 2010)

TY  - CONF
AU  - Mihajlović, L.
AU  - Cirkovic-Velicković, T.
AU  - Jadranin, Milka
AU  - Burazer, Lidija
AU  - Milčić-Matić, Natalija
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/729
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens
VL  - 65
SP  - 331
EP  - 331
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1469
ER  - 
@conference{
author = "Mihajlović, L. and Cirkovic-Velicković, T. and Jadranin, Milka and Burazer, Lidija and Milčić-Matić, Natalija",
year = "2010",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens",
volume = "65",
pages = "331-331",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1469"
}
Mihajlović, L., Cirkovic-Velicković, T., Jadranin, M., Burazer, L.,& Milčić-Matić, N.. (2010). The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 65, 331-331.
https://hdl.handle.net/21.15107/rcub_cherry_1469
Mihajlović L, Cirkovic-Velicković T, Jadranin M, Burazer L, Milčić-Matić N. The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2010;65:331-331.
https://hdl.handle.net/21.15107/rcub_cherry_1469 .
Mihajlović, L., Cirkovic-Velicković, T., Jadranin, Milka, Burazer, Lidija, Milčić-Matić, Natalija, "The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 65 (2010):331-331,
https://hdl.handle.net/21.15107/rcub_cherry_1469 .

Dog saliva - a source of dog allergens

Polović, N.; Bergman, T.; Milčić-Matić, Natalija; Gronlund, H.; van Hage, M.

(Wiley-Blackwell, Hoboken, 2010)

TY  - CONF
AU  - Polović, N.
AU  - Bergman, T.
AU  - Milčić-Matić, Natalija
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/737
AB  - Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - a source of dog allergens
VL  - 65
IS  - 92
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1464
ER  - 
@conference{
author = "Polović, N. and Bergman, T. and Milčić-Matić, Natalija and Gronlund, H. and van Hage, M.",
year = "2010",
abstract = "Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - a source of dog allergens",
volume = "65",
number = "92",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1464"
}
Polović, N., Bergman, T., Milčić-Matić, N., Gronlund, H.,& van Hage, M.. (2010). Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 65(92), 105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464
Polović N, Bergman T, Milčić-Matić N, Gronlund H, van Hage M. Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2010;65(92):105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464 .
Polović, N., Bergman, T., Milčić-Matić, Natalija, Gronlund, H., van Hage, M., "Dog saliva - a source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 65, no. 92 (2010):105-105,
https://hdl.handle.net/21.15107/rcub_cherry_1464 .

Značaj kućnih grinja u kliničkoj praksi malih životinja

Francuski, Jelena; Milčić Matić, Natalija; Popović, N.

(Beograd : Fakultet veterinarske medicine, 2010)

TY  - CONF
AU  - Francuski, Jelena
AU  - Milčić Matić, Natalija
AU  - Popović, N.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3682
PB  - Beograd : Fakultet veterinarske medicine
C3  - Dvanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2010, Subotica, Jun 18 - 20, 2010
T1  - Značaj kućnih grinja u kliničkoj praksi malih životinja
SP  - 57
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3682
ER  - 
@conference{
author = "Francuski, Jelena and Milčić Matić, Natalija and Popović, N.",
year = "2010",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Dvanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2010, Subotica, Jun 18 - 20, 2010",
title = "Značaj kućnih grinja u kliničkoj praksi malih životinja",
pages = "57-58",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3682"
}
Francuski, J., Milčić Matić, N.,& Popović, N.. (2010). Značaj kućnih grinja u kliničkoj praksi malih životinja. in Dvanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2010, Subotica, Jun 18 - 20, 2010
Beograd : Fakultet veterinarske medicine., 57-58.
https://hdl.handle.net/21.15107/rcub_veterinar_3682
Francuski J, Milčić Matić N, Popović N. Značaj kućnih grinja u kliničkoj praksi malih životinja. in Dvanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2010, Subotica, Jun 18 - 20, 2010. 2010;:57-58.
https://hdl.handle.net/21.15107/rcub_veterinar_3682 .
Francuski, Jelena, Milčić Matić, Natalija, Popović, N., "Značaj kućnih grinja u kliničkoj praksi malih životinja" in Dvanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2010, Subotica, Jun 18 - 20, 2010 (2010):57-58,
https://hdl.handle.net/21.15107/rcub_veterinar_3682 .

Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation

Perović, I.; Milovanović, M.; Stanić, Dragana; Burazer, Lidija; Petrović, D.; Milčić-Matić, Natalija; Gafvelink, G.; van Hage, M.; Jankov, R.; Ćirković-Veličković, Tanja

(Wiley, Hoboken, 2009)

TY  - JOUR
AU  - Perović, I.
AU  - Milovanović, M.
AU  - Stanić, Dragana
AU  - Burazer, Lidija
AU  - Petrović, D.
AU  - Milčić-Matić, Natalija
AU  - Gafvelink, G.
AU  - van Hage, M.
AU  - Jankov, R.
AU  - Ćirković-Veličković, Tanja
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/621
AB  - Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.
PB  - Wiley, Hoboken
T2  - Clinical and Experimental Allergy
T1  - Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation
VL  - 39
IS  - 3
SP  - 435
EP  - 446
DO  - 10.1111/j.1365-2222.2008.03158.x
ER  - 
@article{
author = "Perović, I. and Milovanović, M. and Stanić, Dragana and Burazer, Lidija and Petrović, D. and Milčić-Matić, Natalija and Gafvelink, G. and van Hage, M. and Jankov, R. and Ćirković-Veličković, Tanja",
year = "2009",
abstract = "Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.",
publisher = "Wiley, Hoboken",
journal = "Clinical and Experimental Allergy",
title = "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation",
volume = "39",
number = "3",
pages = "435-446",
doi = "10.1111/j.1365-2222.2008.03158.x"
}
Perović, I., Milovanović, M., Stanić, D., Burazer, L., Petrović, D., Milčić-Matić, N., Gafvelink, G., van Hage, M., Jankov, R.,& Ćirković-Veličković, T.. (2009). Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy
Wiley, Hoboken., 39(3), 435-446.
https://doi.org/10.1111/j.1365-2222.2008.03158.x
Perović I, Milovanović M, Stanić D, Burazer L, Petrović D, Milčić-Matić N, Gafvelink G, van Hage M, Jankov R, Ćirković-Veličković T. Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy. 2009;39(3):435-446.
doi:10.1111/j.1365-2222.2008.03158.x .
Perović, I., Milovanović, M., Stanić, Dragana, Burazer, Lidija, Petrović, D., Milčić-Matić, Natalija, Gafvelink, G., van Hage, M., Jankov, R., Ćirković-Veličković, Tanja, "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation" in Clinical and Experimental Allergy, 39, no. 3 (2009):435-446,
https://doi.org/10.1111/j.1365-2222.2008.03158.x . .
9
9
9

Doping kontrola konja u 2008. godini

Milčić Matić, Natalija; Andrić, Nenad; Jovanović, Milan; Trivić, Mirna; Popović, N.

(Beograd : Fakultet veterinarske medicine, 2009)

TY  - CONF
AU  - Milčić Matić, Natalija
AU  - Andrić, Nenad
AU  - Jovanović, Milan
AU  - Trivić, Mirna
AU  - Popović, N.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3668
PB  - Beograd : Fakultet veterinarske medicine
C3  - Jedanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2009, Subotica, Jun 19 - 21, 2009
T1  - Doping kontrola konja u 2008. godini
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3668
ER  - 
@conference{
author = "Milčić Matić, Natalija and Andrić, Nenad and Jovanović, Milan and Trivić, Mirna and Popović, N.",
year = "2009",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Jedanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2009, Subotica, Jun 19 - 21, 2009",
title = "Doping kontrola konja u 2008. godini",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3668"
}
Milčić Matić, N., Andrić, N., Jovanović, M., Trivić, M.,& Popović, N.. (2009). Doping kontrola konja u 2008. godini. in Jedanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2009, Subotica, Jun 19 - 21, 2009
Beograd : Fakultet veterinarske medicine., 90-91.
https://hdl.handle.net/21.15107/rcub_veterinar_3668
Milčić Matić N, Andrić N, Jovanović M, Trivić M, Popović N. Doping kontrola konja u 2008. godini. in Jedanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2009, Subotica, Jun 19 - 21, 2009. 2009;:90-91.
https://hdl.handle.net/21.15107/rcub_veterinar_3668 .
Milčić Matić, Natalija, Andrić, Nenad, Jovanović, Milan, Trivić, Mirna, Popović, N., "Doping kontrola konja u 2008. godini" in Jedanaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2009, Subotica, Jun 19 - 21, 2009 (2009):90-91,
https://hdl.handle.net/21.15107/rcub_veterinar_3668 .