TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Radalj, Andrea
AU  - Marković, Maja
AU  - Lazarević, Miodrag
AU  - Palić, Dušan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2955
AB  - Stress inevitably occurs during any fish handling and manipulation in culturing, research,
or clinical examination situations that require capture and removal of fish from water.
Different stress factors can affect the changes in the relative numbers and function of
cells of the fish immune system. Catching, transportation and over-crowding caused
stress-induced changes in the total number of leukocytes and thrombocytes, as well
as changes in the leukocyte formula in Prussian carp (Carassius gibelio, Bloch, 1782)
presented here. Cytochemical characterization of leukocyte cell types was performed by
applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) and Sudan Black B (SBB)
staining of Prussian carp blood smears. Cytochemical characterization is a rapid and
efficient method for white leukocyte differentiation and insight in their functional status.
Comparison and analysis in Prussian carp hematological parameters from fish with
and without exposure to stressful conditions such as capture, manipulation, transport
and holding, revealed significant differences between stressed and non-stressed fish.
Significant reduction in the total number of thrombocytes and lymphocytes and
the increase in total neutrophil count were observed in stressed animals. However,
differences in total leukocyte number and the number of monocytes were not observed.
Deviations from the estimated reference intervals for Prussian carp hematological
parameters clearly indicated the presence/absence of a stress reaction and to some
extent its intensity. Estimated reference intervals and characterization of morphological
and cytochemical appearance of blood cells form a solid basis for further research of
the cellular immune function and hematology changes in Prussian carp.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Acta Veterinaria
T1  - Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern
VL  - 73
IS  - 2
SP  - 179
EP  - 194
DO  - 10.2478/acve-2023-0014
ER  - 

@article{
author = "Aksentijević, Ksenija and Radalj, Andrea and Marković, Maja and Lazarević, Miodrag and Palić, Dušan",
year = "2023",
abstract = "Stress inevitably occurs during any fish handling and manipulation in culturing, research,
or clinical examination situations that require capture and removal of fish from water.
Different stress factors can affect the changes in the relative numbers and function of
cells of the fish immune system. Catching, transportation and over-crowding caused
stress-induced changes in the total number of leukocytes and thrombocytes, as well
as changes in the leukocyte formula in Prussian carp (Carassius gibelio, Bloch, 1782)
presented here. Cytochemical characterization of leukocyte cell types was performed by
applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) and Sudan Black B (SBB)
staining of Prussian carp blood smears. Cytochemical characterization is a rapid and
efficient method for white leukocyte differentiation and insight in their functional status.
Comparison and analysis in Prussian carp hematological parameters from fish with
and without exposure to stressful conditions such as capture, manipulation, transport
and holding, revealed significant differences between stressed and non-stressed fish.
Significant reduction in the total number of thrombocytes and lymphocytes and
the increase in total neutrophil count were observed in stressed animals. However,
differences in total leukocyte number and the number of monocytes were not observed.
Deviations from the estimated reference intervals for Prussian carp hematological
parameters clearly indicated the presence/absence of a stress reaction and to some
extent its intensity. Estimated reference intervals and characterization of morphological
and cytochemical appearance of blood cells form a solid basis for further research of
the cellular immune function and hematology changes in Prussian carp.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Acta Veterinaria",
title = "Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern",
volume = "73",
number = "2",
pages = "179-194",
doi = "10.2478/acve-2023-0014"
}

Aksentijević, K., Radalj, A., Marković, M., Lazarević, M.,& Palić, D.. (2023). Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern. in Acta Veterinaria
University of Belgrade, Faculty of Veterinary Medicine., 73(2), 179-194.
https://doi.org/10.2478/acve-2023-0014

Aksentijević K, Radalj A, Marković M, Lazarević M, Palić D. Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern. in Acta Veterinaria. 2023;73(2):179-194.
doi:10.2478/acve-2023-0014 .

Aksentijević, Ksenija, Radalj, Andrea, Marković, Maja, Lazarević, Miodrag, Palić, Dušan, "Effects of transport and holding stress on Prussian carp (Carassius gibelio, Bloch, 1782.) leukogram pattern" in Acta Veterinaria, 73, no. 2 (2023):179-194,
https://doi.org/10.2478/acve-2023-0014 . .

TY  - JOUR
AU  - Jakovljević, Goran
AU  - Lazarević, Miodrag
AU  - Mirilović, Milorad
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Šefer, Dragan
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2267
AB  - This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.
AB  - Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.
PB  - Fakultet veterinarske medicine
T2  - Acta veterinaria-Beograd
T1  - The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen
T1  - Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova
VL  - 71
IS  - 3
SP  - 326
EP  - 343
DO  - 10.2478/acve-2021-0028
ER  - 

@article{
author = "Jakovljević, Goran and Lazarević, Miodrag and Mirilović, Milorad and Milovanović, Aleksandar and Apić, Jelena and Šefer, Dragan and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2021",
abstract = "This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage., Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.",
publisher = "Fakultet veterinarske medicine",
journal = "Acta veterinaria-Beograd",
title = "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen, Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova",
volume = "71",
number = "3",
pages = "326-343",
doi = "10.2478/acve-2021-0028"
}

Jakovljević, G., Lazarević, M., Mirilović, M., Milovanović, A., Apić, J., Šefer, D., Nedić, S.,& Vakanjac, S.. (2021). The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd
Fakultet veterinarske medicine., 71(3), 326-343.
https://doi.org/10.2478/acve-2021-0028

Jakovljević G, Lazarević M, Mirilović M, Milovanović A, Apić J, Šefer D, Nedić S, Vakanjac S. The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd. 2021;71(3):326-343.
doi:10.2478/acve-2021-0028 .

Jakovljević, Goran, Lazarević, Miodrag, Mirilović, Milorad, Milovanović, Aleksandar, Apić, Jelena, Šefer, Dragan, Nedić, Svetlana, Vakanjac, Slobodanka, "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen" in Acta veterinaria-Beograd, 71, no. 3 (2021):326-343,
https://doi.org/10.2478/acve-2021-0028 . .

TY  - CONF
AU  - Milovanoviić, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Maksimović, Nevena
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3426
AB  - U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost.
AB  - In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.
PB  - Požarevac : Sitograf RM : Veterinarski specijalistički institut
C3  - 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
T1  - Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača
T1  - Influence of red light spectrum irradiation of boar semen on litter size in sows
SP  - 71
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3426
ER  - 

@conference{
author = "Milovanoviić, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Maksimović, Nevena",
year = "2021",
abstract = "U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost., In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.",
publisher = "Požarevac : Sitograf RM : Veterinarski specijalistički institut",
journal = "18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021",
title = "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača, Influence of red light spectrum irradiation of boar semen on litter size in sows",
pages = "71-72",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3426"
}

Milovanoviić, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M.,& Maksimović, N.. (2021). Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
Požarevac : Sitograf RM : Veterinarski specijalistički institut., 71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426

Milovanoviić A, Barna T, Apić J, Stojanov I, Lazarević M, Maksimović N. Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021. 2021;:71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

Milovanoviić, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Maksimović, Nevena, "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača" in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021 (2021):71-72,
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

TY  - JOUR
AU  - Jaćević, Vesna
AU  - Dumanović, Jelena
AU  - Lazarević, Miodrag
AU  - Nepovimova, Eugenie
AU  - Resanović, Radmila
AU  - Milovanović, Zoran
AU  - Wu, Qinghua
AU  - Kuča, Kamil
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1872
AB  - In this paper, the potential antidote efficacy of commercially available formulations of various feed additives such as Minazel-Plus®, Mycosorb®, and Mycofix® was considered by recording their incidence on general health, body weight, and food and water intake, as well as through histopathology and semiquantitative analysis of gastric alterations in Wistar rats treated with the T-2 toxin in a single-dose regimen of 1.67 mg/kg p.o. (1 LD50) for 4 weeks. As an organic adsorbent, Mycosorb® successfully antagonized acute lethal incidence of the T-2 toxin (protective index (PI) = 2.25; p < 0.05 vs. T-2 toxin), and had adverse effects on body weight gain as well as food and water intake during the research (p < 0.001). However, the protective efficacy of the other two food additives was significantly lower (p < 0.05). Treatment with Mycosorb® significantly reduced the severity of gastric damage, which was not the case when the other two adsorbents were used. Our results suggest that Mycosorb® is a much better adsorbent for preventing the adverse impact of the T-2 toxin as well as its toxic metabolites compared with Minazel-plus® or Mycofix-plus®, and it almost completely suppresses its acute toxic effects and cytotoxic potential on the gastric epithelial, glandular, and vascular endothelial cells. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PB  - MDPI
T2  - Toxins
T1  - Antidotal potency of the novel, structurally different adsorbents in rats acutely intoxicated with the T-2 toxin
VL  - 12
IS  - 10
DO  - 10.3390/toxins12100643
ER  - 

@article{
author = "Jaćević, Vesna and Dumanović, Jelena and Lazarević, Miodrag and Nepovimova, Eugenie and Resanović, Radmila and Milovanović, Zoran and Wu, Qinghua and Kuča, Kamil",
year = "2020",
abstract = "In this paper, the potential antidote efficacy of commercially available formulations of various feed additives such as Minazel-Plus®, Mycosorb®, and Mycofix® was considered by recording their incidence on general health, body weight, and food and water intake, as well as through histopathology and semiquantitative analysis of gastric alterations in Wistar rats treated with the T-2 toxin in a single-dose regimen of 1.67 mg/kg p.o. (1 LD50) for 4 weeks. As an organic adsorbent, Mycosorb® successfully antagonized acute lethal incidence of the T-2 toxin (protective index (PI) = 2.25; p < 0.05 vs. T-2 toxin), and had adverse effects on body weight gain as well as food and water intake during the research (p < 0.001). However, the protective efficacy of the other two food additives was significantly lower (p < 0.05). Treatment with Mycosorb® significantly reduced the severity of gastric damage, which was not the case when the other two adsorbents were used. Our results suggest that Mycosorb® is a much better adsorbent for preventing the adverse impact of the T-2 toxin as well as its toxic metabolites compared with Minazel-plus® or Mycofix-plus®, and it almost completely suppresses its acute toxic effects and cytotoxic potential on the gastric epithelial, glandular, and vascular endothelial cells. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
publisher = "MDPI",
journal = "Toxins",
title = "Antidotal potency of the novel, structurally different adsorbents in rats acutely intoxicated with the T-2 toxin",
volume = "12",
number = "10",
doi = "10.3390/toxins12100643"
}

Jaćević, V., Dumanović, J., Lazarević, M., Nepovimova, E., Resanović, R., Milovanović, Z., Wu, Q.,& Kuča, K.. (2020). Antidotal potency of the novel, structurally different adsorbents in rats acutely intoxicated with the T-2 toxin. in Toxins
MDPI., 12(10).
https://doi.org/10.3390/toxins12100643

Jaćević V, Dumanović J, Lazarević M, Nepovimova E, Resanović R, Milovanović Z, Wu Q, Kuča K. Antidotal potency of the novel, structurally different adsorbents in rats acutely intoxicated with the T-2 toxin. in Toxins. 2020;12(10).
doi:10.3390/toxins12100643 .

Jaćević, Vesna, Dumanović, Jelena, Lazarević, Miodrag, Nepovimova, Eugenie, Resanović, Radmila, Milovanović, Zoran, Wu, Qinghua, Kuča, Kamil, "Antidotal potency of the novel, structurally different adsorbents in rats acutely intoxicated with the T-2 toxin" in Toxins, 12, no. 10 (2020),
https://doi.org/10.3390/toxins12100643 . .

TY  - JOUR
AU  - Samardžija, Marko
AU  - Lojkić, Martina
AU  - Maćešić, Nino
AU  - Valpotić, Hrvoje
AU  - Butković, Ivan
AU  - Šavorić, Juraj
AU  - Žura Žaja, Ivona
AU  - Leiner, Denis
AU  - Đuričić, Dražen
AU  - Marković, Franjo
AU  - Kočila, Predrag
AU  - Vidas, Željko
AU  - Gerenčer, Marijan
AU  - Kaštelan, Andrija
AU  - Milovanović, Aleksandar
AU  - Lazarević, Miodrag
AU  - Rukavina, Daniel
AU  - Valpotić, Ivica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1967
AB  - Literally, reproductive immunology was born in bovine on-farm reproduction where seminal experiments intended for developing methods for embryo transfer in cattle were performed. Actually, these experiments led to two of major concepts and fundamental principles of reproductive immunology using the bovine species as a model for biomedical research, namely the concept of acquired immunological tolerance and the paradox of the semiallogeneic bovine foetus whereby such organism can develop within an immunologically competent host. Peter Medawar, a scientist who together with Frank Macfarlande Burnet shared the 1960 Nobel Prize in physiology or medicine for discovery of acquired immunological tolerance, while studying dizygotic cattle twins, thereby giving birth to reproductive immunology. Also, these findings significantly influenced development of organ transplants and showed that using farm animals as models for studying transplantation immunology had general relevance for mammalian biology and health including those of humans. However, the interest for further research of the fascinating maternal immune influences on pregnancy and perinatal outcomes and of the prevention and treatment of immunologically mediated reproductive disorders in viviparous mammals of veterinary relevance by veterinary immunologists and reproductive clinicians have been very scarce regarding the application of nonspecific immunomodulatory agents for prevention and treatment of subfertility and infertility in pigs and cattle, but still broadening knowledge in this area and hold great potential for improving such therapy in the future. The aim of the current overview is to provide up-to-date information and explaining/translating relevant immunology phenomena into veterinary practice for specialists and scientists/clinicians in reproduction of animals.
PB  - Taylor & Francis
T2  - Veterinary Quarterly
T1  - Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses
VL  - 40
IS  - 1
SP  - 353
EP  - 383
DO  - 10.1080/01652176.2020.1852336
ER  - 

@article{
author = "Samardžija, Marko and Lojkić, Martina and Maćešić, Nino and Valpotić, Hrvoje and Butković, Ivan and Šavorić, Juraj and Žura Žaja, Ivona and Leiner, Denis and Đuričić, Dražen and Marković, Franjo and Kočila, Predrag and Vidas, Željko and Gerenčer, Marijan and Kaštelan, Andrija and Milovanović, Aleksandar and Lazarević, Miodrag and Rukavina, Daniel and Valpotić, Ivica",
year = "2020",
abstract = "Literally, reproductive immunology was born in bovine on-farm reproduction where seminal experiments intended for developing methods for embryo transfer in cattle were performed. Actually, these experiments led to two of major concepts and fundamental principles of reproductive immunology using the bovine species as a model for biomedical research, namely the concept of acquired immunological tolerance and the paradox of the semiallogeneic bovine foetus whereby such organism can develop within an immunologically competent host. Peter Medawar, a scientist who together with Frank Macfarlande Burnet shared the 1960 Nobel Prize in physiology or medicine for discovery of acquired immunological tolerance, while studying dizygotic cattle twins, thereby giving birth to reproductive immunology. Also, these findings significantly influenced development of organ transplants and showed that using farm animals as models for studying transplantation immunology had general relevance for mammalian biology and health including those of humans. However, the interest for further research of the fascinating maternal immune influences on pregnancy and perinatal outcomes and of the prevention and treatment of immunologically mediated reproductive disorders in viviparous mammals of veterinary relevance by veterinary immunologists and reproductive clinicians have been very scarce regarding the application of nonspecific immunomodulatory agents for prevention and treatment of subfertility and infertility in pigs and cattle, but still broadening knowledge in this area and hold great potential for improving such therapy in the future. The aim of the current overview is to provide up-to-date information and explaining/translating relevant immunology phenomena into veterinary practice for specialists and scientists/clinicians in reproduction of animals.",
publisher = "Taylor & Francis",
journal = "Veterinary Quarterly",
title = "Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses",
volume = "40",
number = "1",
pages = "353-383",
doi = "10.1080/01652176.2020.1852336"
}

Samardžija, M., Lojkić, M., Maćešić, N., Valpotić, H., Butković, I., Šavorić, J., Žura Žaja, I., Leiner, D., Đuričić, D., Marković, F., Kočila, P., Vidas, Ž., Gerenčer, M., Kaštelan, A., Milovanović, A., Lazarević, M., Rukavina, D.,& Valpotić, I.. (2020). Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses. in Veterinary Quarterly
Taylor & Francis., 40(1), 353-383.
https://doi.org/10.1080/01652176.2020.1852336

Samardžija M, Lojkić M, Maćešić N, Valpotić H, Butković I, Šavorić J, Žura Žaja I, Leiner D, Đuričić D, Marković F, Kočila P, Vidas Ž, Gerenčer M, Kaštelan A, Milovanović A, Lazarević M, Rukavina D, Valpotić I. Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses. in Veterinary Quarterly. 2020;40(1):353-383.
doi:10.1080/01652176.2020.1852336 .

Samardžija, Marko, Lojkić, Martina, Maćešić, Nino, Valpotić, Hrvoje, Butković, Ivan, Šavorić, Juraj, Žura Žaja, Ivona, Leiner, Denis, Đuričić, Dražen, Marković, Franjo, Kočila, Predrag, Vidas, Željko, Gerenčer, Marijan, Kaštelan, Andrija, Milovanović, Aleksandar, Lazarević, Miodrag, Rukavina, Daniel, Valpotić, Ivica, "Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses" in Veterinary Quarterly, 40, no. 1 (2020):353-383,
https://doi.org/10.1080/01652176.2020.1852336 . .

TY  - JOUR
AU  - Samardžija, Marko
AU  - Lojkić, Martina
AU  - Maćešić, Nino
AU  - Valpotić, Hrvoje
AU  - Butković, Ivan
AU  - Šavorić, Juraj
AU  - Žura Žaja, Ivona
AU  - Leiner, Denis
AU  - Đuričić, Dražen
AU  - Marković, Franjo
AU  - Kočila, Predrag
AU  - Vidas, Željko
AU  - Gerenčer, Marijan
AU  - Kaštelan, Andrija
AU  - Milovanović, Aleksandar
AU  - Lazarević, Miodrag
AU  - Rukavina, Daniel
AU  - Valpotić, Ivica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2052
AB  - In the original manuscript the affiliations for the authors M. Gerencer, A. Kastelan, A. Milovanovic, M. Lazarevic, D. Rukavina, I. Valpotic are set incorrectly. This has now been corrected as shown below. M. Samardzijaa , M. Lojkica , N. Macesica , H. Valpoticb , I. Butkovica , J. Savorica , I. Zura  Zaja  c , D. Leinerd , D. Duri - cice , F. Markovicf , P. Kocilag , Z. Vidas  h , M. Gerencerl , A. Kastelani , A. Milovanovicj , M. Lazarevick , D. Rukavinai and I. Valpotic m a Clinic for Obstetrics and Reproduction of Animals, Veterinary Faculty University of Zagreb, Zagreb, Croatia; b Department for Animal Nutrition and Dietetics, Veterinary Faculty University of Zagreb, Zagreb, Croatia; c Department for Physiology and Radiobiology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; d Department of Anatomy, Histology and Embriology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; e Veterinary Practice, Durd - - evac, Croatia; f Belupo d.d. Danica, Koprivnica, Croatia; g Animal Feed Factory, Cakovec, Croatia; h Faculty of Medicine, Department of Urology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; i Croatian Academy of Sciences and Arts, Croatian Academy of Sciences and Arts, Zagreb, Croatia; j Department of Reproduction, Veterinary Scientific Institute, Novi Sad, Serbia; k Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia; l Department of Cellular Immunology, Baxter Hyland Immuno, Vienna, Austria; mDepartment of Biology, Veterinary Faculty University of Zagreb, Zagreb, Croatia
PB  - Taylor & Francis
T2  - Veterinary Quarterly
T1  - Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.
VL  - 41
IS  - 1
SP  - 152
EP  - 152
DO  - 10.1080/01652176.2020.1852336
ER  - 

@article{
author = "Samardžija, Marko and Lojkić, Martina and Maćešić, Nino and Valpotić, Hrvoje and Butković, Ivan and Šavorić, Juraj and Žura Žaja, Ivona and Leiner, Denis and Đuričić, Dražen and Marković, Franjo and Kočila, Predrag and Vidas, Željko and Gerenčer, Marijan and Kaštelan, Andrija and Milovanović, Aleksandar and Lazarević, Miodrag and Rukavina, Daniel and Valpotić, Ivica",
year = "2020",
abstract = "In the original manuscript the affiliations for the authors M. Gerencer, A. Kastelan, A. Milovanovic, M. Lazarevic, D. Rukavina, I. Valpotic are set incorrectly. This has now been corrected as shown below. M. Samardzijaa , M. Lojkica , N. Macesica , H. Valpoticb , I. Butkovica , J. Savorica , I. Zura  Zaja  c , D. Leinerd , D. Duri - cice , F. Markovicf , P. Kocilag , Z. Vidas  h , M. Gerencerl , A. Kastelani , A. Milovanovicj , M. Lazarevick , D. Rukavinai and I. Valpotic m a Clinic for Obstetrics and Reproduction of Animals, Veterinary Faculty University of Zagreb, Zagreb, Croatia; b Department for Animal Nutrition and Dietetics, Veterinary Faculty University of Zagreb, Zagreb, Croatia; c Department for Physiology and Radiobiology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; d Department of Anatomy, Histology and Embriology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; e Veterinary Practice, Durd - - evac, Croatia; f Belupo d.d. Danica, Koprivnica, Croatia; g Animal Feed Factory, Cakovec, Croatia; h Faculty of Medicine, Department of Urology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; i Croatian Academy of Sciences and Arts, Croatian Academy of Sciences and Arts, Zagreb, Croatia; j Department of Reproduction, Veterinary Scientific Institute, Novi Sad, Serbia; k Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia; l Department of Cellular Immunology, Baxter Hyland Immuno, Vienna, Austria; mDepartment of Biology, Veterinary Faculty University of Zagreb, Zagreb, Croatia",
publisher = "Taylor & Francis",
journal = "Veterinary Quarterly",
title = "Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.",
volume = "41",
number = "1",
pages = "152-152",
doi = "10.1080/01652176.2020.1852336"
}

Samardžija, M., Lojkić, M., Maćešić, N., Valpotić, H., Butković, I., Šavorić, J., Žura Žaja, I., Leiner, D., Đuričić, D., Marković, F., Kočila, P., Vidas, Ž., Gerenčer, M., Kaštelan, A., Milovanović, A., Lazarević, M., Rukavina, D.,& Valpotić, I.. (2020). Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.. in Veterinary Quarterly
Taylor & Francis., 41(1), 152-152.
https://doi.org/10.1080/01652176.2020.1852336

Samardžija M, Lojkić M, Maćešić N, Valpotić H, Butković I, Šavorić J, Žura Žaja I, Leiner D, Đuričić D, Marković F, Kočila P, Vidas Ž, Gerenčer M, Kaštelan A, Milovanović A, Lazarević M, Rukavina D, Valpotić I. Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.. in Veterinary Quarterly. 2020;41(1):152-152.
doi:10.1080/01652176.2020.1852336 .

Samardžija, Marko, Lojkić, Martina, Maćešić, Nino, Valpotić, Hrvoje, Butković, Ivan, Šavorić, Juraj, Žura Žaja, Ivona, Leiner, Denis, Đuričić, Dražen, Marković, Franjo, Kočila, Predrag, Vidas, Željko, Gerenčer, Marijan, Kaštelan, Andrija, Milovanović, Aleksandar, Lazarević, Miodrag, Rukavina, Daniel, Valpotić, Ivica, "Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336." in Veterinary Quarterly, 41, no. 1 (2020):152-152,
https://doi.org/10.1080/01652176.2020.1852336 . .

TY  - CONF
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Vasiljević, Teodora
AU  - Antić, Danko
AU  - Mašić, Aleksandar
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3435
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati
SP  - 142
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3435
ER  - 

@conference{
author = "Milovanović, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Vasiljević, Teodora and Antić, Danko and Mašić, Aleksandar",
year = "2019",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati",
pages = "142-143",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3435"
}

Milovanović, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M., Vasiljević, T., Antić, D.,& Mašić, A.. (2019). Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435

Milovanović A, Barna T, Apić J, Stojanov I, Lazarević M, Vasiljević T, Antić D, Mašić A. Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

Milovanović, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Vasiljević, Teodora, Antić, Danko, Mašić, Aleksandar, "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati" in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):142-143,
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

TY  - JOUR
AU  - Maletić, Milan
AU  - Paprikić, Nevres
AU  - Lazarević, Miodrag
AU  - Hodzić, Aida
AU  - Davidović, Vesna
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1747
AB  - Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle
VL  - 69
IS  - 2
SP  - 153
EP  - 163
DO  - 10.2478/acve-2019-0012
ER  - 

@article{
author = "Maletić, Milan and Paprikić, Nevres and Lazarević, Miodrag and Hodzić, Aida and Davidović, Vesna and Stanišić, Ljubodrag and Stanimirović, Zoran",
year = "2019",
abstract = "Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle",
volume = "69",
number = "2",
pages = "153-163",
doi = "10.2478/acve-2019-0012"
}

Maletić, M., Paprikić, N., Lazarević, M., Hodzić, A., Davidović, V., Stanišić, L.,& Stanimirović, Z.. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 153-163.
https://doi.org/10.2478/acve-2019-0012

Maletić M, Paprikić N, Lazarević M, Hodzić A, Davidović V, Stanišić L, Stanimirović Z. Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd. 2019;69(2):153-163.
doi:10.2478/acve-2019-0012 .

Maletić, Milan, Paprikić, Nevres, Lazarević, Miodrag, Hodzić, Aida, Davidović, Vesna, Stanišić, Ljubodrag, Stanimirović, Zoran, "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle" in Acta Veterinaria-Beograd, 69, no. 2 (2019):153-163,
https://doi.org/10.2478/acve-2019-0012 . .

TY  - JOUR
AU  - Davidović, Vesna
AU  - Jovetić, Branko
AU  - Joksimović-Todorović, Mirjana
AU  - Stojanović, Bojan
AU  - Lazarević, Miodrag
AU  - Perisić, Predrag
AU  - Radivojević, Mihailo
AU  - Maletić, Milan
AU  - Miletić, Aleksandar
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1699
AB  - The aim of this study was to examine the effect of using tannin supplement in ration for mid-lactation dairy cows (90 +/- 17 days in milk) and the number of lactation on metabolic profile parameters values. Additionally, the effect of tannin supplementation on cows production characteristics in the 2nd lactation was evaluated. Research was conducted on 80 Holstein cows divided into two groups of 40 cows (control and experimental group) that included 16 cows in 2nd and 12 in 3rd and 4th lactation. The tannin supplement Tanimil SCC (40 g/cow/day; 40% of tannins) was added in the total mixed ration (TMR) of experimental group, while the control group of cows was fed ration without the tannin supplement. The trial lasted 60 days. Dairy cows, fed diet supplemented with tannin, had markedly lower values (P<0.05) of B-hydroxybutyrate concentration (0.79 vs. 0.96 mmol/L) and lower blood urea concentration (5.89 vs. 6.93 mmol/L). Difference in the blood glucose concentrations (3.92 vs. 3.89 mmol/L) was not significant. The lactation number had no significant effect on values of metabolic profile parameters (concentration of glucose, B-hydroxybutyrate and urea). The effect of interaction of lactation number and tannin supplement on the values of examined parameters of metabolic profile was not significant. Supplementation of tannin in the diet for cows in the second lactation, had a positive effect (P<0.05) on the milk yield (4.42%) and yield of 4% fat corrected milk (FCM, 5.24%). The use of tannin, improved concentration (3.20 vs. 3.12%) and yield (from 1.16 to 1.24 kg/day) of milk protein (P<0.05). Addition of tannin also improved concentration and yield of milk fat, as well as the concentration of lactose. The supplementation of Tanimil SCC to mid-lactation dairy cows had a positive impacts on reduction of blood content of B-hydroxybutyrate and urea, and improved productive performances of cows in the second lactation.
PB  - Univ Ljubljana, Veterinary Faculty, Ljubljana
T2  - Slovenian Veterinary Research
T1  - The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics
VL  - 56
IS  - 4
SP  - 143
EP  - 151
DO  - 10.26873/SVR-552-2019
ER  - 

@article{
author = "Davidović, Vesna and Jovetić, Branko and Joksimović-Todorović, Mirjana and Stojanović, Bojan and Lazarević, Miodrag and Perisić, Predrag and Radivojević, Mihailo and Maletić, Milan and Miletić, Aleksandar",
year = "2019",
abstract = "The aim of this study was to examine the effect of using tannin supplement in ration for mid-lactation dairy cows (90 +/- 17 days in milk) and the number of lactation on metabolic profile parameters values. Additionally, the effect of tannin supplementation on cows production characteristics in the 2nd lactation was evaluated. Research was conducted on 80 Holstein cows divided into two groups of 40 cows (control and experimental group) that included 16 cows in 2nd and 12 in 3rd and 4th lactation. The tannin supplement Tanimil SCC (40 g/cow/day; 40% of tannins) was added in the total mixed ration (TMR) of experimental group, while the control group of cows was fed ration without the tannin supplement. The trial lasted 60 days. Dairy cows, fed diet supplemented with tannin, had markedly lower values (P<0.05) of B-hydroxybutyrate concentration (0.79 vs. 0.96 mmol/L) and lower blood urea concentration (5.89 vs. 6.93 mmol/L). Difference in the blood glucose concentrations (3.92 vs. 3.89 mmol/L) was not significant. The lactation number had no significant effect on values of metabolic profile parameters (concentration of glucose, B-hydroxybutyrate and urea). The effect of interaction of lactation number and tannin supplement on the values of examined parameters of metabolic profile was not significant. Supplementation of tannin in the diet for cows in the second lactation, had a positive effect (P<0.05) on the milk yield (4.42%) and yield of 4% fat corrected milk (FCM, 5.24%). The use of tannin, improved concentration (3.20 vs. 3.12%) and yield (from 1.16 to 1.24 kg/day) of milk protein (P<0.05). Addition of tannin also improved concentration and yield of milk fat, as well as the concentration of lactose. The supplementation of Tanimil SCC to mid-lactation dairy cows had a positive impacts on reduction of blood content of B-hydroxybutyrate and urea, and improved productive performances of cows in the second lactation.",
publisher = "Univ Ljubljana, Veterinary Faculty, Ljubljana",
journal = "Slovenian Veterinary Research",
title = "The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics",
volume = "56",
number = "4",
pages = "143-151",
doi = "10.26873/SVR-552-2019"
}

Davidović, V., Jovetić, B., Joksimović-Todorović, M., Stojanović, B., Lazarević, M., Perisić, P., Radivojević, M., Maletić, M.,& Miletić, A.. (2019). The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics. in Slovenian Veterinary Research
Univ Ljubljana, Veterinary Faculty, Ljubljana., 56(4), 143-151.
https://doi.org/10.26873/SVR-552-2019

Davidović V, Jovetić B, Joksimović-Todorović M, Stojanović B, Lazarević M, Perisić P, Radivojević M, Maletić M, Miletić A. The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics. in Slovenian Veterinary Research. 2019;56(4):143-151.
doi:10.26873/SVR-552-2019 .

Davidović, Vesna, Jovetić, Branko, Joksimović-Todorović, Mirjana, Stojanović, Bojan, Lazarević, Miodrag, Perisić, Predrag, Radivojević, Mihailo, Maletić, Milan, Miletić, Aleksandar, "The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics" in Slovenian Veterinary Research, 56, no. 4 (2019):143-151,
https://doi.org/10.26873/SVR-552-2019 . .

TY  - JOUR
AU  - Trifković, Julijana
AU  - Jovanović, Ljubomir
AU  - Đurić, Miloje
AU  - Stevanovic-Đorđević, Snežana
AU  - Milanović, Svetlana
AU  - Lazarević, Miodrag
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1669
AB  - Season may affect calves thermal comfort and behavior, but the data related to the overall influence of seasonal variations on dams colostrum and postnatal adaptive capability of calves are limited. The aim of this study was to measure the effects of a 49-day-long low air temperature (LAT) season (5.20 +/- 0.46 A degrees C mean air temperature) and a 53-day-long high air temperature (HAT) season (27.40 +/- 0.39 A degrees C mean air temperature) on dams colostrum quality and physiological, biochemical, hormonal, and oxidative stress parameters of their calves during the first 7 days of life. The dams colostrum was sampled at 2, 14, and 26 h after calving, before feeding of their calves. Calves blood samples were taken before the first colostrum intake and on days 1, 2, 3, and 7 of life. Calves physiological parameters were measured on days 0 and 7. HAT season significantly reduced the quality of dams colostrum. The ingestion of the low-quality colostrum, combined with the thermal discomfort during HAT season, probably provoked impaired physiological, biochemical, hormonal, and oxidative stress parameters in samples taken from the post-colostral calves. Additionally, intravenous glucose tolerance test was performed on day 7, which suggested an enhanced insulin response in HAT season calves. This study highlights the importance of adequate supporting strategies for the care of the late gestation cows and postnatal calves during the HAT season.
PB  - Springer, New York
T2  - International Journal of Biometeorology
T1  - Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves
VL  - 62
IS  - 6
SP  - 1097
EP  - 1108
DO  - 10.1007/s00484-018-1514-6
ER  - 

@article{
author = "Trifković, Julijana and Jovanović, Ljubomir and Đurić, Miloje and Stevanovic-Đorđević, Snežana and Milanović, Svetlana and Lazarević, Miodrag and Sladojević, Željko and Kirovski, Danijela",
year = "2018",
abstract = "Season may affect calves thermal comfort and behavior, but the data related to the overall influence of seasonal variations on dams colostrum and postnatal adaptive capability of calves are limited. The aim of this study was to measure the effects of a 49-day-long low air temperature (LAT) season (5.20 +/- 0.46 A degrees C mean air temperature) and a 53-day-long high air temperature (HAT) season (27.40 +/- 0.39 A degrees C mean air temperature) on dams colostrum quality and physiological, biochemical, hormonal, and oxidative stress parameters of their calves during the first 7 days of life. The dams colostrum was sampled at 2, 14, and 26 h after calving, before feeding of their calves. Calves blood samples were taken before the first colostrum intake and on days 1, 2, 3, and 7 of life. Calves physiological parameters were measured on days 0 and 7. HAT season significantly reduced the quality of dams colostrum. The ingestion of the low-quality colostrum, combined with the thermal discomfort during HAT season, probably provoked impaired physiological, biochemical, hormonal, and oxidative stress parameters in samples taken from the post-colostral calves. Additionally, intravenous glucose tolerance test was performed on day 7, which suggested an enhanced insulin response in HAT season calves. This study highlights the importance of adequate supporting strategies for the care of the late gestation cows and postnatal calves during the HAT season.",
publisher = "Springer, New York",
journal = "International Journal of Biometeorology",
title = "Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves",
volume = "62",
number = "6",
pages = "1097-1108",
doi = "10.1007/s00484-018-1514-6"
}

Trifković, J., Jovanović, L., Đurić, M., Stevanovic-Đorđević, S., Milanović, S., Lazarević, M., Sladojević, Ž.,& Kirovski, D.. (2018). Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves. in International Journal of Biometeorology
Springer, New York., 62(6), 1097-1108.
https://doi.org/10.1007/s00484-018-1514-6

Trifković J, Jovanović L, Đurić M, Stevanovic-Đorđević S, Milanović S, Lazarević M, Sladojević Ž, Kirovski D. Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves. in International Journal of Biometeorology. 2018;62(6):1097-1108.
doi:10.1007/s00484-018-1514-6 .

Trifković, Julijana, Jovanović, Ljubomir, Đurić, Miloje, Stevanovic-Đorđević, Snežana, Milanović, Svetlana, Lazarević, Miodrag, Sladojević, Željko, Kirovski, Danijela, "Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves" in International Journal of Biometeorology, 62, no. 6 (2018):1097-1108,
https://doi.org/10.1007/s00484-018-1514-6 . .

TY  - JOUR
AU  - Zekic-Stosić, Marina
AU  - Kanacki, Zdenko
AU  - Stojanović, Dragica
AU  - Bugarski, Dejan
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Masić, Aleksandar
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1593
AB  - Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 +/- 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 +/- 5.22% at the beginning of the study to 89.54 +/- 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with beta-hydroxybutyrat and non-esteri tied fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation
VL  - 12
IS  - 10
SP  - 894
DO  - 10.3855/jidc.10767
ER  - 

@article{
author = "Zekic-Stosić, Marina and Kanacki, Zdenko and Stojanović, Dragica and Bugarski, Dejan and Lazarević, Miodrag and Milovanović, Aleksandar and Masić, Aleksandar",
year = "2018",
abstract = "Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 +/- 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 +/- 5.22% at the beginning of the study to 89.54 +/- 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with beta-hydroxybutyrat and non-esteri tied fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation",
volume = "12",
number = "10",
pages = "894",
doi = "10.3855/jidc.10767"
}

Zekic-Stosić, M., Kanacki, Z., Stojanović, D., Bugarski, D., Lazarević, M., Milovanović, A.,& Masić, A.. (2018). Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(10), 894.
https://doi.org/10.3855/jidc.10767

Zekic-Stosić M, Kanacki Z, Stojanović D, Bugarski D, Lazarević M, Milovanović A, Masić A. Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation. in Journal of Infection in Developing Countries. 2018;12(10):894.
doi:10.3855/jidc.10767 .

Zekic-Stosić, Marina, Kanacki, Zdenko, Stojanović, Dragica, Bugarski, Dejan, Lazarević, Miodrag, Milovanović, Aleksandar, Masić, Aleksandar, "Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation" in Journal of Infection in Developing Countries, 12, no. 10 (2018):894,
https://doi.org/10.3855/jidc.10767 . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2152
AB  - U ovom revijalnom radu su izneta savremena shvatanja mehanizama delovanja,
uloga i mogućnosti primene imunostimulatornih supstanci u veterinarskoj medicini.
Imunofarmakologija je ne tako nova grana farmakologije, nastala iz sve očiglednije
potrebe da se lekovima i drugim prepratima utiče na funkcije imunskog sistema, kako
ljudi tako i životinja. U veterinarskoj medicini inunostimulatori imaju poseban značaj
zbog toga što postoji stalna potreba za zaštitom zdravlja životinja u uslovima intenzivne
proizvodnje. Jedan od najvećih problema u primeni imunomodulatornih supstanci
leži u činjenici da jedna te ista supstancija, u zavisnosti od načina i režima doziranja,
može delovati imunosupresivno ili imunostimulatorno. Ovi efekti zavise i od trenutnog
funkcionalnog stanja imunskog sistema samog pacijenta. Osim prikaza literaturnih
podataka, izneta su i sopstvena iskustva autora proizašla iz brojnih laboratorijskih
ispitivanja i ogleda na farmama. Ona se pre svega odnose na primenu manan-oligosaharida
u povećanju stepena resorpcije kolostralnih imunoglobulina kod teladi i prasadi,
primenu manan-oligosaharida u terapiji puerperalne disgalakcije krmača, primenu
ekstrakta goveđeg timusa kod pacova sa imunosupresijom izazvanom jonizujućim
zracima, primenu ekstrakta zida Mycobacterium phlei (Mcb. phlei) u poboljšanju
reproduktivnih rezultata krmača, primenu ekstrakta ostriga u terapiji endometritisa
krava i primenu visokih doza vitamina C u terapiji subkliničkih oblika mastitisa mlečnih
krava. Takođe je opisana i imunomodulatorna uloga antihelmintika levamizola ispitana
na modelu eksperimentalnog autoimunog encefalomijelitisa pacova kao i uticaj
ekstrakta kukureka (Helleborus odorus Waldst et Kit) na nespecifične odbrambene
mehanizme pacova.
AB  - This review article deals with the contemporary understanding of the action mechanisms,
roles and possibilities for immunostimulatory substances usage in the veterinary
medicine. Immunopharmacology is not that new branch of pharamacology,
raised from the obvious need to influence functions of the immune system both in
humans and animals by medicaments or other substances. In veterinary medicine
inunostimulants are of special significance because there is a permanent need for animal’s
health protection under conditions of intensive production. Immunostimulants
achieved a special significance since the total ban of antibiotics usage as growth stimulators
resulting in reduced health protection, especially in the intensive production.
One of the major problems regarding immunomodulators lays in the fact that the
same drug, depending on the regime and dose, may exert both immunosuppressive
and immunostimulatory effects. These effects are also dependent on the current status
of the immune system functions of the particular patient. In adition to the literature
data, we present our own experiences raised from the numerous laboratory
investigations and field trials. This accomplish usage of mannan-oligosacharides in
enhancing degree of absorption of colostral immunoglobulins in calves and piglets,
application of the bovine thymic extract in irradiated immunosuppressed rats, usage
of the mannan-oligosacharides in the therapy of puerperal dysgalactia in sows, usage
of Mcb. phlei call wall extract in improving reproductive results in sows, usage of the
oyster extract in the therapy of cows endometritis and application of the high vitamin
C doses in the therapy of subclinical mastites in dairy cows. Immunomodulatory effects
of anthelmintic drug, Levamisole, in the model of Experimental Autoimmune Encephalomyelitis
of rats are described as well. In addition, we have investigated effects
of Helleborus odorus (Waldst et Kit) extracts on innate defense mechanisms in rats.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Primena pojedinih imunostimulatora u veterinarskoj medicini
T1  - Application of some immunostimulators in veterinary medicine
VL  - 17
IS  - 1
SP  - 16
EP  - 36
DO  - 10.7251/VETJ1701016L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar",
year = "2017",
abstract = "U ovom revijalnom radu su izneta savremena shvatanja mehanizama delovanja,
uloga i mogućnosti primene imunostimulatornih supstanci u veterinarskoj medicini.
Imunofarmakologija je ne tako nova grana farmakologije, nastala iz sve očiglednije
potrebe da se lekovima i drugim prepratima utiče na funkcije imunskog sistema, kako
ljudi tako i životinja. U veterinarskoj medicini inunostimulatori imaju poseban značaj
zbog toga što postoji stalna potreba za zaštitom zdravlja životinja u uslovima intenzivne
proizvodnje. Jedan od najvećih problema u primeni imunomodulatornih supstanci
leži u činjenici da jedna te ista supstancija, u zavisnosti od načina i režima doziranja,
može delovati imunosupresivno ili imunostimulatorno. Ovi efekti zavise i od trenutnog
funkcionalnog stanja imunskog sistema samog pacijenta. Osim prikaza literaturnih
podataka, izneta su i sopstvena iskustva autora proizašla iz brojnih laboratorijskih
ispitivanja i ogleda na farmama. Ona se pre svega odnose na primenu manan-oligosaharida
u povećanju stepena resorpcije kolostralnih imunoglobulina kod teladi i prasadi,
primenu manan-oligosaharida u terapiji puerperalne disgalakcije krmača, primenu
ekstrakta goveđeg timusa kod pacova sa imunosupresijom izazvanom jonizujućim
zracima, primenu ekstrakta zida Mycobacterium phlei (Mcb. phlei) u poboljšanju
reproduktivnih rezultata krmača, primenu ekstrakta ostriga u terapiji endometritisa
krava i primenu visokih doza vitamina C u terapiji subkliničkih oblika mastitisa mlečnih
krava. Takođe je opisana i imunomodulatorna uloga antihelmintika levamizola ispitana
na modelu eksperimentalnog autoimunog encefalomijelitisa pacova kao i uticaj
ekstrakta kukureka (Helleborus odorus Waldst et Kit) na nespecifične odbrambene
mehanizme pacova., This review article deals with the contemporary understanding of the action mechanisms,
roles and possibilities for immunostimulatory substances usage in the veterinary
medicine. Immunopharmacology is not that new branch of pharamacology,
raised from the obvious need to influence functions of the immune system both in
humans and animals by medicaments or other substances. In veterinary medicine
inunostimulants are of special significance because there is a permanent need for animal’s
health protection under conditions of intensive production. Immunostimulants
achieved a special significance since the total ban of antibiotics usage as growth stimulators
resulting in reduced health protection, especially in the intensive production.
One of the major problems regarding immunomodulators lays in the fact that the
same drug, depending on the regime and dose, may exert both immunosuppressive
and immunostimulatory effects. These effects are also dependent on the current status
of the immune system functions of the particular patient. In adition to the literature
data, we present our own experiences raised from the numerous laboratory
investigations and field trials. This accomplish usage of mannan-oligosacharides in
enhancing degree of absorption of colostral immunoglobulins in calves and piglets,
application of the bovine thymic extract in irradiated immunosuppressed rats, usage
of the mannan-oligosacharides in the therapy of puerperal dysgalactia in sows, usage
of Mcb. phlei call wall extract in improving reproductive results in sows, usage of the
oyster extract in the therapy of cows endometritis and application of the high vitamin
C doses in the therapy of subclinical mastites in dairy cows. Immunomodulatory effects
of anthelmintic drug, Levamisole, in the model of Experimental Autoimmune Encephalomyelitis
of rats are described as well. In addition, we have investigated effects
of Helleborus odorus (Waldst et Kit) extracts on innate defense mechanisms in rats.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Primena pojedinih imunostimulatora u veterinarskoj medicini, Application of some immunostimulators in veterinary medicine",
volume = "17",
number = "1",
pages = "16-36",
doi = "10.7251/VETJ1701016L"
}

Lazarević, M.,& Milovanović, A.. (2017). Primena pojedinih imunostimulatora u veterinarskoj medicini. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(1), 16-36.
https://doi.org/10.7251/VETJ1701016L

Lazarević M, Milovanović A. Primena pojedinih imunostimulatora u veterinarskoj medicini. in Veterinarski žurnal Republike Srpske. 2017;17(1):16-36.
doi:10.7251/VETJ1701016L .

Lazarević, Miodrag, Milovanović, Aleksandar, "Primena pojedinih imunostimulatora u veterinarskoj medicini" in Veterinarski žurnal Republike Srpske, 17, no. 1 (2017):16-36,
https://doi.org/10.7251/VETJ1701016L . .

TY  - CONF
AU  - Milovanović, A.
AU  - Bana, T.
AU  - Lazarević, Miodrag
AU  - Apić, Jelena
AU  - Milanov, Dubravka
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1303
AB  - Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.
PB  - Wiley-Blackwell, Hoboken
C3  - Reproduction in Domestic Animals
T1  - Pararectal treatment of boars with primary semen contamination and astenospermia
VL  - 50
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1303
ER  - 

@conference{
author = "Milovanović, A. and Bana, T. and Lazarević, Miodrag and Apić, Jelena and Milanov, Dubravka",
year = "2015",
abstract = "Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Reproduction in Domestic Animals",
title = "Pararectal treatment of boars with primary semen contamination and astenospermia",
volume = "50",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1303"
}

Milovanović, A., Bana, T., Lazarević, M., Apić, J.,& Milanov, D.. (2015). Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals
Wiley-Blackwell, Hoboken., 50, 67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303

Milovanović A, Bana T, Lazarević M, Apić J, Milanov D. Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals. 2015;50:67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

Milovanović, A., Bana, T., Lazarević, Miodrag, Apić, Jelena, Milanov, Dubravka, "Pararectal treatment of boars with primary semen contamination and astenospermia" in Reproduction in Domestic Animals, 50 (2015):67-67,
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

TY  - JOUR
AU  - Milčić-Matić, Natalija
AU  - Ognjenović, Jana
AU  - Burazer, Lidija
AU  - Blagojević, G.
AU  - Popović, N.
AU  - Lazarević, Miodrag
AU  - Stanić-Vučinić, Dragana
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1062
AB  - Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses.
AB  - Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen
T1  - Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia
VL  - 63
IS  - 4
SP  - 437
EP  - 451
DO  - 10.2298/AVB1304437M
ER  - 

@article{
author = "Milčić-Matić, Natalija and Ognjenović, Jana and Burazer, Lidija and Blagojević, G. and Popović, N. and Lazarević, Miodrag and Stanić-Vučinić, Dragana",
year = "2013",
abstract = "Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causes of pollen-induced allergic reactions both in humans and dogs. It has not been defined yet, what is the major allergen(s) to which most dogs allergic to ragweed show a positive result on intradermal skin test (IDST). In the present study sensitization to Ambrosia artemisiifolia pollen allergens in dogs with atopic dermatitis was examined with both in vivo and in vitro tests, including IDST and serum allergen specific IgE test. Detection of specific-IgE antibodies against ragweed allergens by immunoblotting in the sera of allergic dogs was optimized, as well. Dogs that were positive, as judged by IDST reactions to ragweed pollen allergens, also had alergen specific IgE antibodies in their sera. Results indicate that major allergens of A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Further characterization of ragweed allergens is needed before they could potentially be used in intradermal testing or allergen immunotherapy in affected dogs. Also, we evaluated new Favrots diagnostic criteria for canine atopic dermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might be concluded that proposed criteria are of great assistance for seting up suspected diagnosis of canine atopic dermatitis, after ruling out other pruritic dermatoses., Kratka ambrozija (Ambrosia artemisiifolia) je jedan od najčešćih uzročnika alergijskih reakcija izazvanih polenom kod ljudi i pasa. Još uvek nije definisano koji je glavni alergen (i), na koji, većina pasa alergičnih na polen ambrozije, ispoljava pozitivnu reakciju na intradermalnom testu. U ovoj studiji je ispitana senzibilizacija na polen ove biljke kod pasa sa simptomima atopijskog dermatitisa in vivo i in vitro testovima, uključujući intradermalni test i dokazivanje prisustva alergen specifičnih IgE antitela u serumu. Optimizovani su uslovi za detekciju IgE specifičnih antitela iz seruma pasa alergičnih na polen ambrozije imunoblot tehnikom. Psi koji su imali pozitivnu reakciju na polen ove biljke na intradermalnom testu, takođe su imali specifična IgE antitela u serumu. Dobijeni rezultati ukazuju da su glavni alergeni Ambrosia artemisiifolia kod pasa Amb a 1 i Amb a 2. Neophodna je dalja karakterizacija alergena ambrozije kako bi se oni mogli primeniti pri rutinskom intradermalnom testiranju ili u alergen specifičnoj imunoterapiji obolelih pasa. Takođe je razmatrana i validnost Favrotovih dijagnostičkih kriterijuma kod pasa alergičnih na polen ambrozije. Može se zaključiti da su predloženi kriterijumi od velike pomoći u postavljanju suspektne dijagnoze atopijskog dermatitisa pasa, nakon isključenja drugih pruritičnih dermatoza.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen, Evaluacija kriterijuma za dijagnozu atopijskog dermatitisa i detekcija alergen specifičnih IgE antitela kod pasa alergičnih na polen biljke Ambrosia artemisiifolia",
volume = "63",
number = "4",
pages = "437-451",
doi = "10.2298/AVB1304437M"
}

Milčić-Matić, N., Ognjenović, J., Burazer, L., Blagojević, G., Popović, N., Lazarević, M.,& Stanić-Vučinić, D.. (2013). Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 437-451.
https://doi.org/10.2298/AVB1304437M

Milčić-Matić N, Ognjenović J, Burazer L, Blagojević G, Popović N, Lazarević M, Stanić-Vučinić D. Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen. in Acta Veterinaria-Beograd. 2013;63(4):437-451.
doi:10.2298/AVB1304437M .

Milčić-Matić, Natalija, Ognjenović, Jana, Burazer, Lidija, Blagojević, G., Popović, N., Lazarević, Miodrag, Stanić-Vučinić, Dragana, "Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen" in Acta Veterinaria-Beograd, 63, no. 4 (2013):437-451,
https://doi.org/10.2298/AVB1304437M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Maksimović, N.
AU  - Barna, Tomislav
AU  - Lazarević, Miodrag
AU  - Delić, N.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1025
AB  - This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.
AB  - U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Laparoscopic insemination of sheep in Republic of Serbia
T1  - Laparoskopsko osemenjavanje ovaca u Republici Srbiji
VL  - 29
IS  - 3
SP  - 449
EP  - 456
DO  - 10.2298/BAH1303449M
ER  - 

@article{
author = "Milovanović, Aleksandar and Maksimović, N. and Barna, Tomislav and Lazarević, Miodrag and Delić, N.",
year = "2013",
abstract = "This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks., U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Laparoscopic insemination of sheep in Republic of Serbia, Laparoskopsko osemenjavanje ovaca u Republici Srbiji",
volume = "29",
number = "3",
pages = "449-456",
doi = "10.2298/BAH1303449M"
}

Milovanović, A., Maksimović, N., Barna, T., Lazarević, M.,& Delić, N.. (2013). Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 449-456.
https://doi.org/10.2298/BAH1303449M

Milovanović A, Maksimović N, Barna T, Lazarević M, Delić N. Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry. 2013;29(3):449-456.
doi:10.2298/BAH1303449M .

Milovanović, Aleksandar, Maksimović, N., Barna, Tomislav, Lazarević, Miodrag, Delić, N., "Laparoscopic insemination of sheep in Republic of Serbia" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):449-456,
https://doi.org/10.2298/BAH1303449M . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Suljkanović, A.
AU  - Mickov, Lj.
AU  - Šabanović, Mustafa
AU  - Paprikić, N.
AU  - Mlinar, S.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1059
AB  - The aim of this study was to investigate the influence of antisperm Ig G and Ig A antibodies (ASA) from the sera and cervical mucus of cows on bulls sperm motility. A total of 64 cows was included in the study and samples of sera and cervical mucus were collected on the day of artificial insemination. Cows were of Busha breed or mix breed with Simmental. The presence of antisperm Ig G and Ig A antibodies was determined by indirect immunofluorescence method and according to these results, cows were divided in groups as follows: cows with high or low ASA titer in their sera and cows with high or low ASA titer in the cervical mucus. Influence of antisperm antibodies on sperm motility was further estimated by Computer Assisted Semen Analysis (CASA). Results demonstrated a significant difference in the influence of antisperm antibodies depending on their origin and titer.
AB  - Osnovni cilj ovih istraživanja je bio da se ispita uticaj antitela Ig G i Ig A klase protiv antigena spermatozoida (ASA) iz krvnog seruma i cervikalne sluzi krava na pokretljivost spermatozoida bika. Studijom je bilo obuhvaćeno 64 plotkinje a uzorci seruma i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja. Krave su bile rase buša ili melezi buše sa simentalcem. Titar antitela Ig G i Ig A klase je određivan metodom indirektne imunofluorescence i na osnovu tih rezultata krave su bile podeljene u sledeće grupe: plotkinje sa visokim i niskim titrom ASA u krvnom serumu i plotkinje sa visokim i niskim titrom ASA u cervikalnoj sluzi. Uticaj ASA na pokretljivost spermatozoida je zatim određivan kompjuterskom analizom (Computer Assisted Semen Analisis - CASA). Dobijeni rezultati ukazuju značajne razlike u uticaju ASA na pokretljivost spermatozoida u zavisnosti od njihovog porekla i titra.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility
T1  - Uticaj antispermalnih antitela Ig G i Ig A klase poreklom iz seruma i cervikalne sluzi krava na pokretljivost spermatozoida
VL  - 63
IS  - 5-6
SP  - 499
EP  - 511
DO  - 10.2298/AVB1306499L
ER  - 

@article{
author = "Lazarević, Miodrag and Suljkanović, A. and Mickov, Lj. and Šabanović, Mustafa and Paprikić, N. and Mlinar, S.",
year = "2013",
abstract = "The aim of this study was to investigate the influence of antisperm Ig G and Ig A antibodies (ASA) from the sera and cervical mucus of cows on bulls sperm motility. A total of 64 cows was included in the study and samples of sera and cervical mucus were collected on the day of artificial insemination. Cows were of Busha breed or mix breed with Simmental. The presence of antisperm Ig G and Ig A antibodies was determined by indirect immunofluorescence method and according to these results, cows were divided in groups as follows: cows with high or low ASA titer in their sera and cows with high or low ASA titer in the cervical mucus. Influence of antisperm antibodies on sperm motility was further estimated by Computer Assisted Semen Analysis (CASA). Results demonstrated a significant difference in the influence of antisperm antibodies depending on their origin and titer., Osnovni cilj ovih istraživanja je bio da se ispita uticaj antitela Ig G i Ig A klase protiv antigena spermatozoida (ASA) iz krvnog seruma i cervikalne sluzi krava na pokretljivost spermatozoida bika. Studijom je bilo obuhvaćeno 64 plotkinje a uzorci seruma i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja. Krave su bile rase buša ili melezi buše sa simentalcem. Titar antitela Ig G i Ig A klase je određivan metodom indirektne imunofluorescence i na osnovu tih rezultata krave su bile podeljene u sledeće grupe: plotkinje sa visokim i niskim titrom ASA u krvnom serumu i plotkinje sa visokim i niskim titrom ASA u cervikalnoj sluzi. Uticaj ASA na pokretljivost spermatozoida je zatim određivan kompjuterskom analizom (Computer Assisted Semen Analisis - CASA). Dobijeni rezultati ukazuju značajne razlike u uticaju ASA na pokretljivost spermatozoida u zavisnosti od njihovog porekla i titra.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility, Uticaj antispermalnih antitela Ig G i Ig A klase poreklom iz seruma i cervikalne sluzi krava na pokretljivost spermatozoida",
volume = "63",
number = "5-6",
pages = "499-511",
doi = "10.2298/AVB1306499L"
}

Lazarević, M., Suljkanović, A., Mickov, Lj., Šabanović, M., Paprikić, N.,& Mlinar, S.. (2013). The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 499-511.
https://doi.org/10.2298/AVB1306499L

Lazarević M, Suljkanović A, Mickov L, Šabanović M, Paprikić N, Mlinar S. The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility. in Acta Veterinaria-Beograd. 2013;63(5-6):499-511.
doi:10.2298/AVB1306499L .

Lazarević, Miodrag, Suljkanović, A., Mickov, Lj., Šabanović, Mustafa, Paprikić, N., Mlinar, S., "The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility" in Acta Veterinaria-Beograd, 63, no. 5-6 (2013):499-511,
https://doi.org/10.2298/AVB1306499L . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Milanov, Dubravka
AU  - Lazarević, Miodrag
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1072
AB  - In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality.
AB  - U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control
T1  - Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova
VL  - 6
IS  - 1
SP  - 57
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1072
ER  - 

@article{
author = "Milovanović, Aleksandar and Barna, Tomislav and Milanov, Dubravka and Lazarević, Miodrag",
year = "2013",
abstract = "In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality., U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control, Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova",
volume = "6",
number = "1",
pages = "57-70",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1072"
}

Milovanović, A., Barna, T., Milanov, D.,& Lazarević, M.. (2013). Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 6(1), 57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072

Milovanović A, Barna T, Milanov D, Lazarević M. Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine. 2013;6(1):57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

Milovanović, Aleksandar, Barna, Tomislav, Milanov, Dubravka, Lazarević, Miodrag, "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control" in Arhiv veterinarske medicine, 6, no. 1 (2013):57-70,
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

TY  - JOUR
AU  - Nešić, Vladimir
AU  - Resanović, Radmila
AU  - Marinković, Darko
AU  - Nešić, Ksenija
AU  - Lazarević, Miodrag
AU  - Nešić, Slađan
AU  - Aleksić-Kovačević, Sanja
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/867
AB  - This investigation was conducted in order to investigate the efficacy of the detoxifying agent Mycofix® Plus (MP) in the prevention and/or alleviation in vivo adverse effects of T-2 toxin in broilers. In addition, the adsorbing potential of MP was estimated in vitro. Mean degradation levels of T-2 toxin with MP in vitro, as measured by HPTLC, varied from 26.06 to 31.02 % and the adsorption ability was elevated in acidic environment (pH 3). In vivo trial was performed on 160 one day old 'Ross' broiler chicks and lasted for 21 days. Birds were divided into 4 equal groups as follows: Group 1 - negative control; Group 2 - positive control - 2 ppm T-2 toxin; Group 3 - 2 ppm T-2 toxin+2 kg/t MP; Group 4 - 2 kg/t MP. Broilers fed the diet containing 2 mg/kg of T-2 toxin without MP developed typical T-2 toxicosis. Birds that were fed the diet containing both T-2 and MP had better performances and no oral ulcerations as the dominant sign of T-2 toxicosis were observed. Histopathological examination of tissues originating from birds fed the diet containing T-2 toxin revealed degenerative changes in the oral and small intestine mucosa, necroses of enterocytes and hepatocytes, as well as depletion of lymphocytes in the bursa Fabricii. Immunohistochemical examination also revealed negative effects of T-2 toxin on cells proliferation in intestineal and bile duct mucosa, as well as on lymphocytes from bursa Fabricii. The macroscopic and microscopic structure of the liver, intestine and bursa Fabricii of broilers fed a diet containing T-2 toxin and MP was mostly preserved. Cutaneous basophile hypersensitivity reaction was weaker in broilers fed mixtures containing 2 mg/kg T-2 toxin.
AB  - U cilju ispitivanja efikasnosti detoksikujućeg agensa Mycofix® Plus (MP) na preveniranje ili ublažavanje štetnih efekata T-2 toksikoze kod brojlera izvedena su ispitivanja u in vitro uslovima, kao i in vivo ogled. Prosečne vrednosti degradacije T-2 toksina od strane MP u in vitro uslovima, ustanovljene HPTLC metodom, su iznosile 26,06 do 31,02 %, pri čemu je konstatovana veća sposobnost adsorpcije ovog toksina u kiseloj sredini (pH 3). In vivo ogled je bio postavljen na 160 jednodnevnih brojlera provenijencije 'Ross', podeljenih u 4 eksperimentalne grupe sa po 40 jedinki u svakoj i trajao je 21 dan. Praćenjem zdravstvenog stanja, kliničke slike i proizvodnih rezultata, kod brojlera koji su u hrani dobijali umešan T-2 toksin u količini od 2 mg/kg, konstatovani su karakteristični simptomi za T-2 toksikozu. Kod brojlera koji su uz toksin, hranom dobijali iMP, konstatovani su zaštitni efekti u vidu izostanka ulceracija u usnoj duplji i boljih proizvodnih rezultata u odnosu na brojlere koji su dobijali samo T-2 toksin. Patohistološkim ispitivanjima uočeni su negativni efekti T-2 toksina u svim ispitivanim organima u vidu degenerativnih promena na sluzokoži usne duplje i tankog creva, nekroza enterocita i hepatocita kao i deplecija limfocita u Fabricijevoj burzi. Imunohistohemijskim ispitivanjima uočeni su negativni efekti T-2 toksina na proliferativnu aktivnost ćelija mukoze creva, žučnih kanala i limfocita u Fabricijevoj burzi. Kod brojlera koji su putem hrane dobijali T-2 toksin i adsorbens MP, zapaža se uglavnom očuvana makroskopska i mikroskopska struktura jetre, creva i Fabricijeve burze. Reakcija kožne preosetljivosti na PHA je bila slabija kod brojlera koji su hranom dobijali samo T-2 toksin.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Efficacy of T-2 toxin detoxifying agent in broiler chickens
T1  - Efikasnost detoksikujućeg agensa kod T-2 toksikoze brojlera
VL  - 62
IS  - 2-3
SP  - 171
EP  - 182
DO  - 10.2298/AVB1203171N
ER  - 

@article{
author = "Nešić, Vladimir and Resanović, Radmila and Marinković, Darko and Nešić, Ksenija and Lazarević, Miodrag and Nešić, Slađan and Aleksić-Kovačević, Sanja",
year = "2012",
abstract = "This investigation was conducted in order to investigate the efficacy of the detoxifying agent Mycofix® Plus (MP) in the prevention and/or alleviation in vivo adverse effects of T-2 toxin in broilers. In addition, the adsorbing potential of MP was estimated in vitro. Mean degradation levels of T-2 toxin with MP in vitro, as measured by HPTLC, varied from 26.06 to 31.02 % and the adsorption ability was elevated in acidic environment (pH 3). In vivo trial was performed on 160 one day old 'Ross' broiler chicks and lasted for 21 days. Birds were divided into 4 equal groups as follows: Group 1 - negative control; Group 2 - positive control - 2 ppm T-2 toxin; Group 3 - 2 ppm T-2 toxin+2 kg/t MP; Group 4 - 2 kg/t MP. Broilers fed the diet containing 2 mg/kg of T-2 toxin without MP developed typical T-2 toxicosis. Birds that were fed the diet containing both T-2 and MP had better performances and no oral ulcerations as the dominant sign of T-2 toxicosis were observed. Histopathological examination of tissues originating from birds fed the diet containing T-2 toxin revealed degenerative changes in the oral and small intestine mucosa, necroses of enterocytes and hepatocytes, as well as depletion of lymphocytes in the bursa Fabricii. Immunohistochemical examination also revealed negative effects of T-2 toxin on cells proliferation in intestineal and bile duct mucosa, as well as on lymphocytes from bursa Fabricii. The macroscopic and microscopic structure of the liver, intestine and bursa Fabricii of broilers fed a diet containing T-2 toxin and MP was mostly preserved. Cutaneous basophile hypersensitivity reaction was weaker in broilers fed mixtures containing 2 mg/kg T-2 toxin., U cilju ispitivanja efikasnosti detoksikujućeg agensa Mycofix® Plus (MP) na preveniranje ili ublažavanje štetnih efekata T-2 toksikoze kod brojlera izvedena su ispitivanja u in vitro uslovima, kao i in vivo ogled. Prosečne vrednosti degradacije T-2 toksina od strane MP u in vitro uslovima, ustanovljene HPTLC metodom, su iznosile 26,06 do 31,02 %, pri čemu je konstatovana veća sposobnost adsorpcije ovog toksina u kiseloj sredini (pH 3). In vivo ogled je bio postavljen na 160 jednodnevnih brojlera provenijencije 'Ross', podeljenih u 4 eksperimentalne grupe sa po 40 jedinki u svakoj i trajao je 21 dan. Praćenjem zdravstvenog stanja, kliničke slike i proizvodnih rezultata, kod brojlera koji su u hrani dobijali umešan T-2 toksin u količini od 2 mg/kg, konstatovani su karakteristični simptomi za T-2 toksikozu. Kod brojlera koji su uz toksin, hranom dobijali iMP, konstatovani su zaštitni efekti u vidu izostanka ulceracija u usnoj duplji i boljih proizvodnih rezultata u odnosu na brojlere koji su dobijali samo T-2 toksin. Patohistološkim ispitivanjima uočeni su negativni efekti T-2 toksina u svim ispitivanim organima u vidu degenerativnih promena na sluzokoži usne duplje i tankog creva, nekroza enterocita i hepatocita kao i deplecija limfocita u Fabricijevoj burzi. Imunohistohemijskim ispitivanjima uočeni su negativni efekti T-2 toksina na proliferativnu aktivnost ćelija mukoze creva, žučnih kanala i limfocita u Fabricijevoj burzi. Kod brojlera koji su putem hrane dobijali T-2 toksin i adsorbens MP, zapaža se uglavnom očuvana makroskopska i mikroskopska struktura jetre, creva i Fabricijeve burze. Reakcija kožne preosetljivosti na PHA je bila slabija kod brojlera koji su hranom dobijali samo T-2 toksin.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Efficacy of T-2 toxin detoxifying agent in broiler chickens, Efikasnost detoksikujućeg agensa kod T-2 toksikoze brojlera",
volume = "62",
number = "2-3",
pages = "171-182",
doi = "10.2298/AVB1203171N"
}

Nešić, V., Resanović, R., Marinković, D., Nešić, K., Lazarević, M., Nešić, S.,& Aleksić-Kovačević, S.. (2012). Efficacy of T-2 toxin detoxifying agent in broiler chickens. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(2-3), 171-182.
https://doi.org/10.2298/AVB1203171N

Nešić V, Resanović R, Marinković D, Nešić K, Lazarević M, Nešić S, Aleksić-Kovačević S. Efficacy of T-2 toxin detoxifying agent in broiler chickens. in Acta Veterinaria-Beograd. 2012;62(2-3):171-182.
doi:10.2298/AVB1203171N .

Nešić, Vladimir, Resanović, Radmila, Marinković, Darko, Nešić, Ksenija, Lazarević, Miodrag, Nešić, Slađan, Aleksić-Kovačević, Sanja, "Efficacy of T-2 toxin detoxifying agent in broiler chickens" in Acta Veterinaria-Beograd, 62, no. 2-3 (2012):171-182,
https://doi.org/10.2298/AVB1203171N . .

TY  - JOUR
AU  - Lauš, S.
AU  - Trailović, Ružica
AU  - Đoković, Stefan
AU  - Lazarević, Miodrag
AU  - Trailović, Dragiša
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/921
AB  - The comparison of some serum protein concentrations was performed on 12 Yugoslav Trotter mares and their newborn foals. The mares included in the evaluation were divided into two groups of 6 each. The mares in the first group were vaccinated against equine herpes virus 1 and 4, in the 5th, 7th and 9th month of pregnancy, while mares in the second group were not vaccinated at all. Pregnant mares were clinically observed during the last stage of pregnancy and blood for biochemical evaluations was sampled immediately after foaling. Foals were clinically observed for seven days after birth and blood samples were collected immediately after foaling (before nursing), and 24, 48, 72 and 168 hours after birth. Foals included in the evaluation were divided into two groups according to the group allocation of the respective mares. All mares gave birth to normal foals in expected terms. Biochemical examination revealed slightly lower total gammaglobulin and IgG values in tested mares compared to the values obtained in other horse breeds. The antibody titres against equine herpes virus-1 reached the level that provides sufficient protection in vaccinated mares. Gammaglobulin and traces of IgG were present in the blood serum of foals tested immediately after birth and before nursing. A significant increase of IgG and gammaglobulin concentration was revealed in all foals after the first 24 hours of life. The observed first day increase of concentration was followed by stagnation of gammaglobulin and IgG levels in all foals. Total protein values showed a significant increase 24 hours after the first intake of colostrum in all foals. Immunoglobulin G concentration established by semiquantitative test was considered low positive in 16.67% and in 33.34% of foals from vaccinated and unvaccinated mares, respectively. Turbidimetric analyses of the same samples revealed sufficient Ig transfer, i.e. Ig concentration over 8 g/L. Comparison of the results obtained by the two methods indicates that semi-quantitative field test results were clinically valid. There were no antibodies against EHV 1 in foals immediately after birth and before the first colostrum intake, and a highly significant increase of serum antibody level was recordered 24 hours after the onset of nursing in foals born from vaccinated mothers.
AB  - Ispitivanja su sprovedena na 12 kobila rase jugoslovenski kasač i njihovih 12 ždrebadi. Prva grupa od šest kobila je trokratno vakcinisana protiv EHV-1/4 tokom graviditeta, dok je druga grupa bila nevakcinisana. Od svih kobila su neposredno posle ždrebljenja uzeti uzorci krvi za biohemijski pregled. Novorođ ena ždrebad su klinički opservirana tokom prvih 7 dana života, uz uzimanje uzoraka krvi pre uzimanja kolostruma, zatim nakon 24, 48, 72 i 168 časova. Kod svih kobila su utvrđene nešto niže vrednosti gama globulina i IgG od vrednosti koje su zabeležene kod drugih rasa, pri čemu su vrednosti titra antitela na EHV-1 kod vakcinisanih kobila bile na nivou koji pruža zadovoljavajuću zaštitu, za razliku od nevakcinisanih kobila koje su bile ili seronegativne ili imale minimalan titar anti EHV-1 antitela. Kod većine ždrebadi je neposredno posle rođenja a pre prvog napoja u krvnom serumu ustanovljeno prisustvo gama globulina i IgG u tragovima, pri čemu je statistički značajno povećanje i gama globulina i IgG ustanovljeno posle prvih 24 časa. Primenom semikvantitativnog testa za određivanje IgG kod 25% ždrebadi utvrđene su niže vrednosti IgG (slabo pozitivne - što ukazuje na vrednosti od 5-8 g/L), iako je koncentracija IgG utvrđena imunoturbidimetrijom i kod njih bila veća od 8 g/L. Pre prvog unosa kolostruma u serumu ždrebadi nema antitela protiv EHV-1.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Comparative analysis of some serum proteins and immunoglobulin G concentration in the blood of Yugoslav Trotter mares and newborn foals
T1  - Uporedno ispitivanje koncentracije pojedinih frakcija serumskih proteina i imunoglobulina klase G u krvnom serumu kobila i novorođene ždrebadi jugoslovenskog kasača
VL  - 62
IS  - 5-6
SP  - 569
EP  - 578
DO  - 10.2298/AVB1206569L
ER  - 

@article{
author = "Lauš, S. and Trailović, Ružica and Đoković, Stefan and Lazarević, Miodrag and Trailović, Dragiša",
year = "2012",
abstract = "The comparison of some serum protein concentrations was performed on 12 Yugoslav Trotter mares and their newborn foals. The mares included in the evaluation were divided into two groups of 6 each. The mares in the first group were vaccinated against equine herpes virus 1 and 4, in the 5th, 7th and 9th month of pregnancy, while mares in the second group were not vaccinated at all. Pregnant mares were clinically observed during the last stage of pregnancy and blood for biochemical evaluations was sampled immediately after foaling. Foals were clinically observed for seven days after birth and blood samples were collected immediately after foaling (before nursing), and 24, 48, 72 and 168 hours after birth. Foals included in the evaluation were divided into two groups according to the group allocation of the respective mares. All mares gave birth to normal foals in expected terms. Biochemical examination revealed slightly lower total gammaglobulin and IgG values in tested mares compared to the values obtained in other horse breeds. The antibody titres against equine herpes virus-1 reached the level that provides sufficient protection in vaccinated mares. Gammaglobulin and traces of IgG were present in the blood serum of foals tested immediately after birth and before nursing. A significant increase of IgG and gammaglobulin concentration was revealed in all foals after the first 24 hours of life. The observed first day increase of concentration was followed by stagnation of gammaglobulin and IgG levels in all foals. Total protein values showed a significant increase 24 hours after the first intake of colostrum in all foals. Immunoglobulin G concentration established by semiquantitative test was considered low positive in 16.67% and in 33.34% of foals from vaccinated and unvaccinated mares, respectively. Turbidimetric analyses of the same samples revealed sufficient Ig transfer, i.e. Ig concentration over 8 g/L. Comparison of the results obtained by the two methods indicates that semi-quantitative field test results were clinically valid. There were no antibodies against EHV 1 in foals immediately after birth and before the first colostrum intake, and a highly significant increase of serum antibody level was recordered 24 hours after the onset of nursing in foals born from vaccinated mothers., Ispitivanja su sprovedena na 12 kobila rase jugoslovenski kasač i njihovih 12 ždrebadi. Prva grupa od šest kobila je trokratno vakcinisana protiv EHV-1/4 tokom graviditeta, dok je druga grupa bila nevakcinisana. Od svih kobila su neposredno posle ždrebljenja uzeti uzorci krvi za biohemijski pregled. Novorođ ena ždrebad su klinički opservirana tokom prvih 7 dana života, uz uzimanje uzoraka krvi pre uzimanja kolostruma, zatim nakon 24, 48, 72 i 168 časova. Kod svih kobila su utvrđene nešto niže vrednosti gama globulina i IgG od vrednosti koje su zabeležene kod drugih rasa, pri čemu su vrednosti titra antitela na EHV-1 kod vakcinisanih kobila bile na nivou koji pruža zadovoljavajuću zaštitu, za razliku od nevakcinisanih kobila koje su bile ili seronegativne ili imale minimalan titar anti EHV-1 antitela. Kod većine ždrebadi je neposredno posle rođenja a pre prvog napoja u krvnom serumu ustanovljeno prisustvo gama globulina i IgG u tragovima, pri čemu je statistički značajno povećanje i gama globulina i IgG ustanovljeno posle prvih 24 časa. Primenom semikvantitativnog testa za određivanje IgG kod 25% ždrebadi utvrđene su niže vrednosti IgG (slabo pozitivne - što ukazuje na vrednosti od 5-8 g/L), iako je koncentracija IgG utvrđena imunoturbidimetrijom i kod njih bila veća od 8 g/L. Pre prvog unosa kolostruma u serumu ždrebadi nema antitela protiv EHV-1.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Comparative analysis of some serum proteins and immunoglobulin G concentration in the blood of Yugoslav Trotter mares and newborn foals, Uporedno ispitivanje koncentracije pojedinih frakcija serumskih proteina i imunoglobulina klase G u krvnom serumu kobila i novorođene ždrebadi jugoslovenskog kasača",
volume = "62",
number = "5-6",
pages = "569-578",
doi = "10.2298/AVB1206569L"
}

Lauš, S., Trailović, R., Đoković, S., Lazarević, M.,& Trailović, D.. (2012). Comparative analysis of some serum proteins and immunoglobulin G concentration in the blood of Yugoslav Trotter mares and newborn foals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 569-578.
https://doi.org/10.2298/AVB1206569L

Lauš S, Trailović R, Đoković S, Lazarević M, Trailović D. Comparative analysis of some serum proteins and immunoglobulin G concentration in the blood of Yugoslav Trotter mares and newborn foals. in Acta Veterinaria-Beograd. 2012;62(5-6):569-578.
doi:10.2298/AVB1206569L .

Lauš, S., Trailović, Ružica, Đoković, Stefan, Lazarević, Miodrag, Trailović, Dragiša, "Comparative analysis of some serum proteins and immunoglobulin G concentration in the blood of Yugoslav Trotter mares and newborn foals" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):569-578,
https://doi.org/10.2298/AVB1206569L . .

TY  - JOUR
AU  - Kirovski, Danijela
AU  - Sladojević, Željko
AU  - Stojić, Velibor
AU  - Vujanac, Ivan
AU  - Lazarević, Miodrag
AU  - Radovanović, Anita
AU  - Savić, Đ.
AU  - Nedić, Olgica
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/860
AB  - The objective of this study was to examine the effect of dietary energy supplementation on hormones that are considered to be the main signals of a shift in energy balance around parturition. Sixty dry cows, 15 days before calving, were chosen and divided into two eaqual groups: control and experimental (GLY). Both groups were fed a standard ration balanced in accordance to the stage of the productive reproductive cycle. Additionally, each cow in the GLY group was given glycerol based dietary energy supplementation (250 mL daily during the dry and 300 mL daily during the lactation period), which provided additional 9.30 MJ NEL during the dry and 13.95 MJ NEL during the early lactation period. Milk production was measured on days 30 and 60 of lactation and milk production was significantly higher in GLY compared to control group at day 60 of lactation (p lt 0.05). Service period and insemination index were used as reproductive outcome parameters. Average service period in the control group was significantly longer than in the GLY group (p lt 0.05). Average insemination index in the control group was not significantly different than the index obtained for the GLY group. Blood samples were taken before the begining of the experiment (15 days before parturition), and at days 7, 30 and 60 of lactation. Concentrations of thyroid hormones, IGF-I, relative abundance of IGFBP-2, IGFBP-3 and IGFBP-4, concentrations of total protein and albumin in the blood were measured. Results showed that at days 7 and 30 after parturition, T4 concentrations were significantly higher (p lt 0.001, respectively) in GLY than in the control group, while T3 concentrations were significantly higher in GLY group only at day 7 after parturition (p lt 0.001). IGF-I concentrations and IGFBP-3 abundance were significantly higher in the GLY compared to the control group in all three examined postpartum periods. IGFBP-2 and IGFBP-4 concentrations were higher in GLY compared to the control group in all three examined postpartum periods, but the difference was significant only on day 60 after parturition (p lt 0.01, respectively). Concentrations of total protein and albumin were significantly higher in GLY compared to the control group in all three examined periods after parturition. Based on these results it can be concluded that peripartum dietary energy supplementation prevent cows' exposure to severe negative energy balance, preserves synthetic activity of hepatocytes and consequently has a positive impact on milk production and reproductive performances in dairy cows.
AB  - Cilj ovog rada je bio da se ispita uticaj energetskog dodatka u ishrani krava na koncentraciju hormona u krvi koji su u periodu oko teljenja glavni pokazatelji promena u energetskom statusu. U tu svrhu, petnaest dana pre teljenja odabrano je 60 krava koje su podeljene u dve jednake grupe: kontrolnu i oglednu (GLY). Obe grupe krava su dobijale identičan obrok usklađen sa proizvodno reproduktivnim ciklusom. Dodatno, kravama GLY grupe je tokom poslednje dve nedelje zasušenja i do 60. dana laktacije dodavan energetski dodatak na bazi glicerola (250 mL dnevno tokom zasušenja odnosno 300 mL nakon teljenja), obezbeđujući dodatnih 9,30 MJ NEL tokom zasušenja, odnosno 13,95 MJ NEL tokom rane laktacije. Kod svih krava je izmerena prosečna dnevna proizvodnja mleka 30. i 60. dana lakatcije, koja je kod GLY grupe bila značajno viša 60. dana (p lt 0,05). Kao pokazatelji reproduktivnog statusa koriščeni su servis period i indeks osemenjavanja. Servis period je bio značajno duži kod kontrolne u odnosu na GLY grupu (p lt 0,05), a vrednost indeksa osemenjavanja se nije značajno razlikovala između grupa. Uzorci krvi krava uzeti su neposredno pre početka ogleda, odnosno 15 dana pre teljenja, kao i 7, 30. i 60. dana laktacije i u njima je određivana koncentracija tireoidnih hormona, IGF-I, relativna zastupljenost IGFBP-2, IGFBP-3 i IGFBP-4, koncentracija ukupnih proteina i albumina. Rezultati su ukazali da je 7. i 30. dana nakon teljenja GLY grupa imala značajno višu koncentraciju T4 (p lt 0,001, pojedinačno) u odnosu na kontrolu, dok je koncentarcija T3 bila značajno viša kod GLY grupe 7. dana nakon teljenja (p lt 0,001). Koncentracija IGF-I i zastupljenost IGFBP-3 je bila značajno viša u krvi krava GLY grupe u odnosu na kontrolu u sva tri ispitivana perioda posle teljenja. Zastupljenost IGFBP-2 i IGFBP-4 je bila viša u krvi krava GLY u odnosu na kontrolnu grupu u sva tri ispitivana perioda postapartalno ali je ova razlika bila značajna jedino 60. dana lakatcije (p lt 0.01, pojedinačno). Koncentracija ukupnih proteina i albumina je, takođe, bila značajno viša u krvi krava GLY grupe u odnosu na kontrolnu grupu u sva tri ispitivana perioda posle teljenja. Na osnovu dobijenih rezultata može se zaključiti da energetski dodatak u ishrani sprečava izloženost krava izrazitom negativnom energetskom bilansu, održava sintetsku funkciju hepatocita i posledično ima pozitivan uticaj na mlečnost i reproduktivne pokazatelje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows
T1  - Uticaj energetskog dodatka u ishrani na koncentraciju tireoidnih hormona, insulinu sličnog faktora rasta-i i njegovih vezujućih proteina u krvi krava tokom rane laktacije
VL  - 62
IS  - 4
SP  - 403
EP  - 419
DO  - 10.2298/AVB1204403K
ER  - 

@article{
author = "Kirovski, Danijela and Sladojević, Željko and Stojić, Velibor and Vujanac, Ivan and Lazarević, Miodrag and Radovanović, Anita and Savić, Đ. and Nedić, Olgica",
year = "2012",
abstract = "The objective of this study was to examine the effect of dietary energy supplementation on hormones that are considered to be the main signals of a shift in energy balance around parturition. Sixty dry cows, 15 days before calving, were chosen and divided into two eaqual groups: control and experimental (GLY). Both groups were fed a standard ration balanced in accordance to the stage of the productive reproductive cycle. Additionally, each cow in the GLY group was given glycerol based dietary energy supplementation (250 mL daily during the dry and 300 mL daily during the lactation period), which provided additional 9.30 MJ NEL during the dry and 13.95 MJ NEL during the early lactation period. Milk production was measured on days 30 and 60 of lactation and milk production was significantly higher in GLY compared to control group at day 60 of lactation (p lt 0.05). Service period and insemination index were used as reproductive outcome parameters. Average service period in the control group was significantly longer than in the GLY group (p lt 0.05). Average insemination index in the control group was not significantly different than the index obtained for the GLY group. Blood samples were taken before the begining of the experiment (15 days before parturition), and at days 7, 30 and 60 of lactation. Concentrations of thyroid hormones, IGF-I, relative abundance of IGFBP-2, IGFBP-3 and IGFBP-4, concentrations of total protein and albumin in the blood were measured. Results showed that at days 7 and 30 after parturition, T4 concentrations were significantly higher (p lt 0.001, respectively) in GLY than in the control group, while T3 concentrations were significantly higher in GLY group only at day 7 after parturition (p lt 0.001). IGF-I concentrations and IGFBP-3 abundance were significantly higher in the GLY compared to the control group in all three examined postpartum periods. IGFBP-2 and IGFBP-4 concentrations were higher in GLY compared to the control group in all three examined postpartum periods, but the difference was significant only on day 60 after parturition (p lt 0.01, respectively). Concentrations of total protein and albumin were significantly higher in GLY compared to the control group in all three examined periods after parturition. Based on these results it can be concluded that peripartum dietary energy supplementation prevent cows' exposure to severe negative energy balance, preserves synthetic activity of hepatocytes and consequently has a positive impact on milk production and reproductive performances in dairy cows., Cilj ovog rada je bio da se ispita uticaj energetskog dodatka u ishrani krava na koncentraciju hormona u krvi koji su u periodu oko teljenja glavni pokazatelji promena u energetskom statusu. U tu svrhu, petnaest dana pre teljenja odabrano je 60 krava koje su podeljene u dve jednake grupe: kontrolnu i oglednu (GLY). Obe grupe krava su dobijale identičan obrok usklađen sa proizvodno reproduktivnim ciklusom. Dodatno, kravama GLY grupe je tokom poslednje dve nedelje zasušenja i do 60. dana laktacije dodavan energetski dodatak na bazi glicerola (250 mL dnevno tokom zasušenja odnosno 300 mL nakon teljenja), obezbeđujući dodatnih 9,30 MJ NEL tokom zasušenja, odnosno 13,95 MJ NEL tokom rane laktacije. Kod svih krava je izmerena prosečna dnevna proizvodnja mleka 30. i 60. dana lakatcije, koja je kod GLY grupe bila značajno viša 60. dana (p lt 0,05). Kao pokazatelji reproduktivnog statusa koriščeni su servis period i indeks osemenjavanja. Servis period je bio značajno duži kod kontrolne u odnosu na GLY grupu (p lt 0,05), a vrednost indeksa osemenjavanja se nije značajno razlikovala između grupa. Uzorci krvi krava uzeti su neposredno pre početka ogleda, odnosno 15 dana pre teljenja, kao i 7, 30. i 60. dana laktacije i u njima je određivana koncentracija tireoidnih hormona, IGF-I, relativna zastupljenost IGFBP-2, IGFBP-3 i IGFBP-4, koncentracija ukupnih proteina i albumina. Rezultati su ukazali da je 7. i 30. dana nakon teljenja GLY grupa imala značajno višu koncentraciju T4 (p lt 0,001, pojedinačno) u odnosu na kontrolu, dok je koncentarcija T3 bila značajno viša kod GLY grupe 7. dana nakon teljenja (p lt 0,001). Koncentracija IGF-I i zastupljenost IGFBP-3 je bila značajno viša u krvi krava GLY grupe u odnosu na kontrolu u sva tri ispitivana perioda posle teljenja. Zastupljenost IGFBP-2 i IGFBP-4 je bila viša u krvi krava GLY u odnosu na kontrolnu grupu u sva tri ispitivana perioda postapartalno ali je ova razlika bila značajna jedino 60. dana lakatcije (p lt 0.01, pojedinačno). Koncentracija ukupnih proteina i albumina je, takođe, bila značajno viša u krvi krava GLY grupe u odnosu na kontrolnu grupu u sva tri ispitivana perioda posle teljenja. Na osnovu dobijenih rezultata može se zaključiti da energetski dodatak u ishrani sprečava izloženost krava izrazitom negativnom energetskom bilansu, održava sintetsku funkciju hepatocita i posledično ima pozitivan uticaj na mlečnost i reproduktivne pokazatelje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows, Uticaj energetskog dodatka u ishrani na koncentraciju tireoidnih hormona, insulinu sličnog faktora rasta-i i njegovih vezujućih proteina u krvi krava tokom rane laktacije",
volume = "62",
number = "4",
pages = "403-419",
doi = "10.2298/AVB1204403K"
}

Kirovski, D., Sladojević, Ž., Stojić, V., Vujanac, I., Lazarević, M., Radovanović, A., Savić, Đ.,& Nedić, O.. (2012). Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 403-419.
https://doi.org/10.2298/AVB1204403K

Kirovski D, Sladojević Ž, Stojić V, Vujanac I, Lazarević M, Radovanović A, Savić Đ, Nedić O. Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows. in Acta Veterinaria-Beograd. 2012;62(4):403-419.
doi:10.2298/AVB1204403K .

Kirovski, Danijela, Sladojević, Željko, Stojić, Velibor, Vujanac, Ivan, Lazarević, Miodrag, Radovanović, Anita, Savić, Đ., Nedić, Olgica, "Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows" in Acta Veterinaria-Beograd, 62, no. 4 (2012):403-419,
https://doi.org/10.2298/AVB1204403K . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Miljas, N.
AU  - Milanov, Dubravka
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/917
AB  - On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle.
AB  - Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution
T1  - Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca
VL  - 62
IS  - 5-6
SP  - 611
EP  - 626
DO  - 10.2298/AVB1206611L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar and Barna, Tomislav and Miljas, N. and Milanov, Dubravka",
year = "2012",
abstract = "On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle., Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution, Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca",
volume = "62",
number = "5-6",
pages = "611-626",
doi = "10.2298/AVB1206611L"
}

Lazarević, M., Milovanović, A., Barna, T., Miljas, N.,& Milanov, D.. (2012). Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 611-626.
https://doi.org/10.2298/AVB1206611L

Lazarević M, Milovanović A, Barna T, Miljas N, Milanov D. Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd. 2012;62(5-6):611-626.
doi:10.2298/AVB1206611L .

Lazarević, Miodrag, Milovanović, Aleksandar, Barna, Tomislav, Miljas, N., Milanov, Dubravka, "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):611-626,
https://doi.org/10.2298/AVB1206611L . .

TY  - JOUR
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Gledić, Dušan
AU  - Lazarević, Miodrag
AU  - Stojanović, S.
AU  - Kanački, Z.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/784
AB  - The aim of this paper is to examine the effect of different sound stress duration on adrenal gland of broiler chickens of different age. The experiments were conducted on 90 HYBRO broiler chickens, divided into nine groups (each group consisting of 10 birds) by different age and lenght of sound stress duration. The chickens were exposed to sound stress in sound attenuated building using a fire alarm bell (95 dB) for stress sessions lasted 120 min every day. The histological structure of adrenal gland was analyzed by light microscopy. The results indicate that adrenal gland is sensitive on this kind of stress and sound as stressor could introduce organism in stress reaction. Adrenal glands of broilers exposed to sound stress characterized by marked hyperemia, hypertrophy of interrenal cells, smaller affinity for staining of chromaffin cells compared with control groups. According to our results, degree of histological changes of adrenal gland under the influence of sound stress depends on the length of exposure and age of chickens.
AB  - Cilj ovog rada je da ispita uticaj različite dužine trajanja zvučnog stresa na histološku građu nadbubrežnih žlezda brojlerskih pilića u različitim uzrastima. Ogled je izveden na 90 HYBRO brojlerskih pilića, podeljenih u 9 grupa u skladu sa uzrastom i dužinom izlaganja stresu. Pilići su zvučnom stresu bili izlagani u zvučno izolovanim objektima uz korišćenje požarnog alarmnog zvona (95dB) u trajanju od 120 min svakog dana. Histološka građa nadbubrežnih žlezda je analizirana svetlosnim mikroskopom. Rezultati ukazuju da je nadbubrežna žlezda osetljiva na ovu vrstu stresa i da zvuk, kao stresor, može da uvede organizam u stresnu reakciju. Nadbubrežne žlezde pilića izlaganih zvučnom stresu karakteriše značajna hiperemija, hipertrofija interrenalnih ćelija, manji afinitet prema bojenju hromafinih ćelija u odnosu na kontrolnu grupu. U skladu sa ovim rezultatima, stepen histoloških promena nadbubrežne žlezde pod uticajem zvučnog stresa zavisi i od dužine izlaganja i uzrasta pilića.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The influence of long term sound stress on histological structure of broiler's adrenal glands
T1  - Uticaj dugotrajnog zvučnog stresa na histološku građu nadbubrežne žlezde brojlera
VL  - 27
IS  - 4
SP  - 1613
EP  - 1619
DO  - 10.2298/BAH1104613Z
ER  - 

@article{
author = "Žikić, Dragan and Ušćebrka, Gordana and Gledić, Dušan and Lazarević, Miodrag and Stojanović, S. and Kanački, Z.",
year = "2011",
abstract = "The aim of this paper is to examine the effect of different sound stress duration on adrenal gland of broiler chickens of different age. The experiments were conducted on 90 HYBRO broiler chickens, divided into nine groups (each group consisting of 10 birds) by different age and lenght of sound stress duration. The chickens were exposed to sound stress in sound attenuated building using a fire alarm bell (95 dB) for stress sessions lasted 120 min every day. The histological structure of adrenal gland was analyzed by light microscopy. The results indicate that adrenal gland is sensitive on this kind of stress and sound as stressor could introduce organism in stress reaction. Adrenal glands of broilers exposed to sound stress characterized by marked hyperemia, hypertrophy of interrenal cells, smaller affinity for staining of chromaffin cells compared with control groups. According to our results, degree of histological changes of adrenal gland under the influence of sound stress depends on the length of exposure and age of chickens., Cilj ovog rada je da ispita uticaj različite dužine trajanja zvučnog stresa na histološku građu nadbubrežnih žlezda brojlerskih pilića u različitim uzrastima. Ogled je izveden na 90 HYBRO brojlerskih pilića, podeljenih u 9 grupa u skladu sa uzrastom i dužinom izlaganja stresu. Pilići su zvučnom stresu bili izlagani u zvučno izolovanim objektima uz korišćenje požarnog alarmnog zvona (95dB) u trajanju od 120 min svakog dana. Histološka građa nadbubrežnih žlezda je analizirana svetlosnim mikroskopom. Rezultati ukazuju da je nadbubrežna žlezda osetljiva na ovu vrstu stresa i da zvuk, kao stresor, može da uvede organizam u stresnu reakciju. Nadbubrežne žlezde pilića izlaganih zvučnom stresu karakteriše značajna hiperemija, hipertrofija interrenalnih ćelija, manji afinitet prema bojenju hromafinih ćelija u odnosu na kontrolnu grupu. U skladu sa ovim rezultatima, stepen histoloških promena nadbubrežne žlezde pod uticajem zvučnog stresa zavisi i od dužine izlaganja i uzrasta pilića.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The influence of long term sound stress on histological structure of broiler's adrenal glands, Uticaj dugotrajnog zvučnog stresa na histološku građu nadbubrežne žlezde brojlera",
volume = "27",
number = "4",
pages = "1613-1619",
doi = "10.2298/BAH1104613Z"
}

Žikić, D., Ušćebrka, G., Gledić, D., Lazarević, M., Stojanović, S.,& Kanački, Z.. (2011). The influence of long term sound stress on histological structure of broiler's adrenal glands. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 27(4), 1613-1619.
https://doi.org/10.2298/BAH1104613Z

Žikić D, Ušćebrka G, Gledić D, Lazarević M, Stojanović S, Kanački Z. The influence of long term sound stress on histological structure of broiler's adrenal glands. in Biotechnology in Animal Husbandry. 2011;27(4):1613-1619.
doi:10.2298/BAH1104613Z .

Žikić, Dragan, Ušćebrka, Gordana, Gledić, Dušan, Lazarević, Miodrag, Stojanović, S., Kanački, Z., "The influence of long term sound stress on histological structure of broiler's adrenal glands" in Biotechnology in Animal Husbandry, 27, no. 4 (2011):1613-1619,
https://doi.org/10.2298/BAH1104613Z . .

TY  - JOUR
AU  - Kirovski, Danijela
AU  - Lazarević, Miodrag
AU  - Stojić, Velibor
AU  - Šamanc, Horea
AU  - Vujanac, Ivan
AU  - Nedić, Olgica
AU  - Masnikosa, Romana
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/808
AB  - The aim of this study was to examine changes in some hormones concentrations in calves during the first 32 hours of neonatal life and to estimate their association with glycemia. Thyrty two Holstein breed calves were selected for the study. Blood samples were taken at 30, 60 and 90 minutes postnatal. Calves received pooled colostrum: primary colostum (1.5 L, 2 hours after birth), secondary colostrum (2 L, 14 hours after birth) and tertiary colostrum (2 L, 26 hours after birth). Blood samples were taken at hours 5, 20 and 32 of neonatal life. Concentrations of glucose, insulin, cortisol, thyroid hormones and IGF-I and abundance of IGFBP-1, IGFBP-2 and IGFBP-3 were determined in the blood serum. The T3/T4 ratio was also calculated. Calves were born hypoglycemic (glycemia was 2.56±1.05 mmol/L at birth). Thereafter, glycemia significantly increased (p lt 0.001) to 3.05±0.89 mmol/L at min 90. Glucose concentration showed a further increase after colostrum intake and was significantly higher than at the initial value in all examined periods (p lt 0.001). During the first 90 minutes of neonatal life insulinemia decreased significantly (p lt 0.001) compared to initial value (26.33±10.05 μIU/L) and it measured 18.66±5.56 μIU/L at min 90. Cortisolemia was highest at minute 30 (85.08±19.36 nmol/L) and than decreased until the end of the experiment (p lt 0.001) compared to initial values in samples obtained during the period of colostrum intake. A significantly high correlation was determined between glycemia and cortisolemia in all examined periods before the first colostrums intake (r2=0.854; p lt 0.01 at min 30; r2=0.742; p lt 0.01 at min 60 and r2=0.551; p lt 0.01 at min 90). T4 concentrations significantly increased during the first 2 hours, while T3 concentrations decreased, significantly from min 30 to min 90 postnatal (p lt 0.05). T3/T4 ratio significantly increased during the first 2 hours of neonatal life. After first colostrum intake, concentrations of both hormones rose significantly compared to the initial level, but T3/T4 ratio did not change and maintained the value determined at minute 90. IGF- 1 concentrations significantly decreased during the first 2 postnatal hours. A significant positive correlation was observed between IGF-1 concentration and insulinemia (r2=0.463; p lt 0.05 at min 30, r2=0.662; p lt 0.01 at min 60 and r2=0.583; p lt 0.01 at min 90). IGFBP-3 abundance significantly decreased, while IGFBP-1 significantly increased in this period. IGFBP-2 abundance was highest at birth. Results presented in this study indicate that the increase in glucose concentration during the first 2 hours of neonatal life, before the first colostrum intake is mainly the result of increased activity of the adrenal cortex in cortisol secretion and extrathyroidal tissue thus providing sufficient triiodothyronine. Immaturity of mechanisms responsible for insulin secretion provides the dominance of catabolic processes. Changes of the IGF system provide a rise of glucose concentration and establishment of energy balance.
AB  - Cilj ovog rada je bio da se ispitaju promene koncentracije pojedinih hormona kod novorođene teladi u prvim satima neonatalnog života i utvrdi njihov uticaj na glikemiju. Odabrana su 32 novorođena teleta Holštajn rase kojima je 30, 60. i 90. minuta postnatalnog života uzeta krv. Telad su bila napajana pulovima kolostruma. Pul primarnog kolostruma davan je u količinama od po 1,5 litar 2 sata nakon rođenja, dok su pulovi sekundarnog i tercijarnog kolostruma davani 12, odnosno 24 sata kasnije, u količinama od po 2 litra. Tokom perioda kolostralnog napoja, teladi je uzorkovana krv 5, 20 i 32. sata nakon rođenja. U uzorcima krvi određ ivana je koncentracija glukoze, insulina, kortizola, tireoidnih hormona i IGF-I, kao i zastupljenost IGFBP-1, IGFBP-2 i IGFBP-3. Takođe je obračunat indeks konverzije T3 u T4. Telad su bila rođena u stanju hipoglikemije (koncentracija glukoze na rođenju je iznosila 2,56 ± 1,05 mmol/l). Nakon toga, glikemija je značajno porasla (p lt 0,001) do 3,05 ±0,89 mmol/l (90. minut). Porast koncentracije glukoze je nastavljen i nakon unosa kolostruma, tako da je glikemija u svim ispitivanim uzorcima bila značajno veća u odnosu na početnu vrednost (p lt 0,001). Tokom prvih 90 minuta neonatalnog života, koncentracija insulina se značajno smanjivala (p lt  0,001) u odnosu na početnu vrednost (26,33 ± 10,05 μIU/l) tako da je 90. minuta postnatalnog života bila 18,66 ± 5,56 μIU/l. Porast insulinemije nakon unosa kolostruma nije bio značajan u odnosu na vrednost određenu 90. minuta. Koncentracija kortizola je bila najviša 30 minuta nakon teljenja (85,08 ± 19,36 nmol/l) a zatim je opadala do kraja perioda ispitivanja i to značajno u odnosu na početnu vrednost (p lt 0,001) u uzorcima dobijenim nakon unosa kolostruma. Visoka pozitivna korelacija je utvrđena između glikemije i kortizolemije u svim ispitivanim terminima pre kolostralnog napoja (r2 = 0,854 u 30. minutu; r2 = 0,742 u 60. minutu i r2= 0,551 u 90. minutu). Koncentracija T4 je značajno rasla tokom prva dva sata neonatalnog života, dok se koncentracija T3 smanjila, značajno od 30. do 90. minuta neonatalnog života (p lt 0,05). Konverzija T3 u T4 je značajno porasla tokom prva dva sata života. Nakon unosa kolostruma, koncentracija oba tireoidna hormona se povećavala (značajno u odnosu na početnu vrednost) a indeks konverzije se nije menjao, već se zadržao na vrednosti ustanovljenoj 90. minuta života. Koncentracija IGF-1 se značajno smanjivala tokom prva 2 sata neonatalnog života. Koncentracija IGF-1 je bila u visokoj pozitivnoj korelaciji sa insulinemijom (r2= 0,463 za 30. minut, r2=0,662 za 60. minut i r2=0,583 za 90. minut). Zastupljenost IGFBP-3 se značajno smanjivala, dok se zastupljenost IGFBP-1 značajno povećavala u ovom periodu. Zastupljenost IGFBP-2 je bila najveća na rođenju. Rezultati prikazani u ovom radu ukazuju da je porast glikemije u prvim satima života, pre unosa kolostruma, prevashodno posledica pojačane aktivnosti kore nadbubrega u sekreciji kortizola i dejodinaza u ekstratireoidnim tkivima koje obezbeđuju povećanu sintezu T3. Sistemi odgovorni za sintezu insulina nisu potpuno funkcionalni u ovom periodu, omogućavajući prevagu kataboličkih u odnosu na anaboličke procese. Promene unutar IGF sistema omogućavaju porast glikemije i uspostavljanje energetske ravnoteže.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life
T1  - Hormonalni status i regulacija glikemije kod novorođene teladi tokom prvih sati postnatalnog života
VL  - 61
IS  - 4
SP  - 349
EP  - 361
DO  - 10.2298/AVB1104349K
ER  - 

@article{
author = "Kirovski, Danijela and Lazarević, Miodrag and Stojić, Velibor and Šamanc, Horea and Vujanac, Ivan and Nedić, Olgica and Masnikosa, Romana",
year = "2011",
abstract = "The aim of this study was to examine changes in some hormones concentrations in calves during the first 32 hours of neonatal life and to estimate their association with glycemia. Thyrty two Holstein breed calves were selected for the study. Blood samples were taken at 30, 60 and 90 minutes postnatal. Calves received pooled colostrum: primary colostum (1.5 L, 2 hours after birth), secondary colostrum (2 L, 14 hours after birth) and tertiary colostrum (2 L, 26 hours after birth). Blood samples were taken at hours 5, 20 and 32 of neonatal life. Concentrations of glucose, insulin, cortisol, thyroid hormones and IGF-I and abundance of IGFBP-1, IGFBP-2 and IGFBP-3 were determined in the blood serum. The T3/T4 ratio was also calculated. Calves were born hypoglycemic (glycemia was 2.56±1.05 mmol/L at birth). Thereafter, glycemia significantly increased (p lt 0.001) to 3.05±0.89 mmol/L at min 90. Glucose concentration showed a further increase after colostrum intake and was significantly higher than at the initial value in all examined periods (p lt 0.001). During the first 90 minutes of neonatal life insulinemia decreased significantly (p lt 0.001) compared to initial value (26.33±10.05 μIU/L) and it measured 18.66±5.56 μIU/L at min 90. Cortisolemia was highest at minute 30 (85.08±19.36 nmol/L) and than decreased until the end of the experiment (p lt 0.001) compared to initial values in samples obtained during the period of colostrum intake. A significantly high correlation was determined between glycemia and cortisolemia in all examined periods before the first colostrums intake (r2=0.854; p lt 0.01 at min 30; r2=0.742; p lt 0.01 at min 60 and r2=0.551; p lt 0.01 at min 90). T4 concentrations significantly increased during the first 2 hours, while T3 concentrations decreased, significantly from min 30 to min 90 postnatal (p lt 0.05). T3/T4 ratio significantly increased during the first 2 hours of neonatal life. After first colostrum intake, concentrations of both hormones rose significantly compared to the initial level, but T3/T4 ratio did not change and maintained the value determined at minute 90. IGF- 1 concentrations significantly decreased during the first 2 postnatal hours. A significant positive correlation was observed between IGF-1 concentration and insulinemia (r2=0.463; p lt 0.05 at min 30, r2=0.662; p lt 0.01 at min 60 and r2=0.583; p lt 0.01 at min 90). IGFBP-3 abundance significantly decreased, while IGFBP-1 significantly increased in this period. IGFBP-2 abundance was highest at birth. Results presented in this study indicate that the increase in glucose concentration during the first 2 hours of neonatal life, before the first colostrum intake is mainly the result of increased activity of the adrenal cortex in cortisol secretion and extrathyroidal tissue thus providing sufficient triiodothyronine. Immaturity of mechanisms responsible for insulin secretion provides the dominance of catabolic processes. Changes of the IGF system provide a rise of glucose concentration and establishment of energy balance., Cilj ovog rada je bio da se ispitaju promene koncentracije pojedinih hormona kod novorođene teladi u prvim satima neonatalnog života i utvrdi njihov uticaj na glikemiju. Odabrana su 32 novorođena teleta Holštajn rase kojima je 30, 60. i 90. minuta postnatalnog života uzeta krv. Telad su bila napajana pulovima kolostruma. Pul primarnog kolostruma davan je u količinama od po 1,5 litar 2 sata nakon rođenja, dok su pulovi sekundarnog i tercijarnog kolostruma davani 12, odnosno 24 sata kasnije, u količinama od po 2 litra. Tokom perioda kolostralnog napoja, teladi je uzorkovana krv 5, 20 i 32. sata nakon rođenja. U uzorcima krvi određ ivana je koncentracija glukoze, insulina, kortizola, tireoidnih hormona i IGF-I, kao i zastupljenost IGFBP-1, IGFBP-2 i IGFBP-3. Takođe je obračunat indeks konverzije T3 u T4. Telad su bila rođena u stanju hipoglikemije (koncentracija glukoze na rođenju je iznosila 2,56 ± 1,05 mmol/l). Nakon toga, glikemija je značajno porasla (p lt 0,001) do 3,05 ±0,89 mmol/l (90. minut). Porast koncentracije glukoze je nastavljen i nakon unosa kolostruma, tako da je glikemija u svim ispitivanim uzorcima bila značajno veća u odnosu na početnu vrednost (p lt 0,001). Tokom prvih 90 minuta neonatalnog života, koncentracija insulina se značajno smanjivala (p lt  0,001) u odnosu na početnu vrednost (26,33 ± 10,05 μIU/l) tako da je 90. minuta postnatalnog života bila 18,66 ± 5,56 μIU/l. Porast insulinemije nakon unosa kolostruma nije bio značajan u odnosu na vrednost određenu 90. minuta. Koncentracija kortizola je bila najviša 30 minuta nakon teljenja (85,08 ± 19,36 nmol/l) a zatim je opadala do kraja perioda ispitivanja i to značajno u odnosu na početnu vrednost (p lt 0,001) u uzorcima dobijenim nakon unosa kolostruma. Visoka pozitivna korelacija je utvrđena između glikemije i kortizolemije u svim ispitivanim terminima pre kolostralnog napoja (r2 = 0,854 u 30. minutu; r2 = 0,742 u 60. minutu i r2= 0,551 u 90. minutu). Koncentracija T4 je značajno rasla tokom prva dva sata neonatalnog života, dok se koncentracija T3 smanjila, značajno od 30. do 90. minuta neonatalnog života (p lt 0,05). Konverzija T3 u T4 je značajno porasla tokom prva dva sata života. Nakon unosa kolostruma, koncentracija oba tireoidna hormona se povećavala (značajno u odnosu na početnu vrednost) a indeks konverzije se nije menjao, već se zadržao na vrednosti ustanovljenoj 90. minuta života. Koncentracija IGF-1 se značajno smanjivala tokom prva 2 sata neonatalnog života. Koncentracija IGF-1 je bila u visokoj pozitivnoj korelaciji sa insulinemijom (r2= 0,463 za 30. minut, r2=0,662 za 60. minut i r2=0,583 za 90. minut). Zastupljenost IGFBP-3 se značajno smanjivala, dok se zastupljenost IGFBP-1 značajno povećavala u ovom periodu. Zastupljenost IGFBP-2 je bila najveća na rođenju. Rezultati prikazani u ovom radu ukazuju da je porast glikemije u prvim satima života, pre unosa kolostruma, prevashodno posledica pojačane aktivnosti kore nadbubrega u sekreciji kortizola i dejodinaza u ekstratireoidnim tkivima koje obezbeđuju povećanu sintezu T3. Sistemi odgovorni za sintezu insulina nisu potpuno funkcionalni u ovom periodu, omogućavajući prevagu kataboličkih u odnosu na anaboličke procese. Promene unutar IGF sistema omogućavaju porast glikemije i uspostavljanje energetske ravnoteže.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life, Hormonalni status i regulacija glikemije kod novorođene teladi tokom prvih sati postnatalnog života",
volume = "61",
number = "4",
pages = "349-361",
doi = "10.2298/AVB1104349K"
}

Kirovski, D., Lazarević, M., Stojić, V., Šamanc, H., Vujanac, I., Nedić, O.,& Masnikosa, R.. (2011). Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(4), 349-361.
https://doi.org/10.2298/AVB1104349K

Kirovski D, Lazarević M, Stojić V, Šamanc H, Vujanac I, Nedić O, Masnikosa R. Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life. in Acta Veterinaria-Beograd. 2011;61(4):349-361.
doi:10.2298/AVB1104349K .

Kirovski, Danijela, Lazarević, Miodrag, Stojić, Velibor, Šamanc, Horea, Vujanac, Ivan, Nedić, Olgica, Masnikosa, Romana, "Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life" in Acta Veterinaria-Beograd, 61, no. 4 (2011):349-361,
https://doi.org/10.2298/AVB1104349K . .

TY  - JOUR
AU  - Milčić-Matić, Natalija
AU  - Popović, N.
AU  - Lazarević, Miodrag
AU  - Medenica, Ljiljana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/669
AB  - Pollens from Ambrosia species have a strong allergenic potential and they represent a complex mixture of at least 52 different antigens. This weed is widespread in Serbia and has a long pollination period (appr. 100 days). Pollens are present in very high concentrations during the pollination season (up to 300 pg/m3 of air). Fifty dogs with a clinical diagnosis of canine atopic dermatitis (CAD) and positive intradermal skin test (IDST) reactions to other environmental allergens were included in this study along with 30 dogs without clinical signs of CAD. Both groups were tested intradermally with Ambrosia artemisiifolia extract in concentration of 1000 PNU/mL (provided by ''Institute of virology, vaccines and sera - Torlak''). We have found that Ambrosia extract was second most common allergen to yield positive intradermal test reactions just after house dust mites. The positive reaction of IDST was observed in 66% of the atopic dogs and in 10% of clinically healthy dogs. Dogs that had a positive reaction on intradermal skin test to Ambrosia pollens, mostly showed secondary skin lesions on the pads and perioral skin. In 88% of cases, Malassezia pachydermatis have been isolated from the skin in an increased number and 64% of dogs had otitis externa. These results indicate, that allergens from Ambrosia artemisifolia are of great importance in the etiopathogenesis of atopic dermatitis in dogs originating from the Belgrade region.
AB  - Polen ambrozije ima jaka alergijska svojstva i sastoji se od kompleksne mešavine od do sada poznatih 52 antigena. Ova korovska biljka je znatno rasprostranjena u Srbiji i ima dugačak period polinacije od prosečno 100 dana. Polen je prisutan u izrazito visokim koncentracijama tokom avgusta i septembra ide i do 300 polenovih zrna u m3 vazduha. Ovim ispitivanjem obuhvaćeno je 50 pasa kod kojih je dijagnostikovan atopijski dermatitis na osnovu kliničkih simptoma i pozitivne reakcije na intradermalnom testu, kao i 30 pasa bez klinički manifestnih simptoma atopijskog dermatitisa. Kod obe grupe pasa uradjeno je intradermalno testiranje standardnim rastvorima proizvođača Instituta za imunologiju i virusologiju Torlak, kao i vodenim rastvorom alergena ambrozije u koncentraciji 1000 PNU/ml. U našem ispitivanju polen ambrozije predstavlja drugi alergen po učestalosti pozitivne reakcije na intradermalnom testu kod ispitivanih pasa, odmah posle kućnih grinja. Pozitivna reakcija zabeležena je kod 66% pasa iz grupe atopičara i kod 10% klinički zdravih pasa. Kod pasa koji su pokazali pozitivnu reakciju na intradermalnom testu na polen ambrozije, sekundarne lezije kože su se najčešće javljale na šapama i oko usana. Kod 88% ovih pasa na koži je u povećanom broju dijagnostikovana Malassezia pachydermatis, a kod 64% jedinki je registrovan otitis externa različite etiologije. Na osnovu dobijenih rezultata se može zaključiti da je alergen biljke Ambrosia artemisiifolia veoma značajan u indukciji atopijskog dermatitisa kod populacije pasa sa šireg područja Beograda.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis
T1  - Značaj alergena biljke Ambrosia artemisiifolia u etiopatogenezi atopijskog dermatitisa pasa
VL  - 60
IS  - 2-3
SP  - 183
EP  - 196
DO  - 10.2298/AVB1003183M
ER  - 

@article{
author = "Milčić-Matić, Natalija and Popović, N. and Lazarević, Miodrag and Medenica, Ljiljana",
year = "2010",
abstract = "Pollens from Ambrosia species have a strong allergenic potential and they represent a complex mixture of at least 52 different antigens. This weed is widespread in Serbia and has a long pollination period (appr. 100 days). Pollens are present in very high concentrations during the pollination season (up to 300 pg/m3 of air). Fifty dogs with a clinical diagnosis of canine atopic dermatitis (CAD) and positive intradermal skin test (IDST) reactions to other environmental allergens were included in this study along with 30 dogs without clinical signs of CAD. Both groups were tested intradermally with Ambrosia artemisiifolia extract in concentration of 1000 PNU/mL (provided by ''Institute of virology, vaccines and sera - Torlak''). We have found that Ambrosia extract was second most common allergen to yield positive intradermal test reactions just after house dust mites. The positive reaction of IDST was observed in 66% of the atopic dogs and in 10% of clinically healthy dogs. Dogs that had a positive reaction on intradermal skin test to Ambrosia pollens, mostly showed secondary skin lesions on the pads and perioral skin. In 88% of cases, Malassezia pachydermatis have been isolated from the skin in an increased number and 64% of dogs had otitis externa. These results indicate, that allergens from Ambrosia artemisifolia are of great importance in the etiopathogenesis of atopic dermatitis in dogs originating from the Belgrade region., Polen ambrozije ima jaka alergijska svojstva i sastoji se od kompleksne mešavine od do sada poznatih 52 antigena. Ova korovska biljka je znatno rasprostranjena u Srbiji i ima dugačak period polinacije od prosečno 100 dana. Polen je prisutan u izrazito visokim koncentracijama tokom avgusta i septembra ide i do 300 polenovih zrna u m3 vazduha. Ovim ispitivanjem obuhvaćeno je 50 pasa kod kojih je dijagnostikovan atopijski dermatitis na osnovu kliničkih simptoma i pozitivne reakcije na intradermalnom testu, kao i 30 pasa bez klinički manifestnih simptoma atopijskog dermatitisa. Kod obe grupe pasa uradjeno je intradermalno testiranje standardnim rastvorima proizvođača Instituta za imunologiju i virusologiju Torlak, kao i vodenim rastvorom alergena ambrozije u koncentraciji 1000 PNU/ml. U našem ispitivanju polen ambrozije predstavlja drugi alergen po učestalosti pozitivne reakcije na intradermalnom testu kod ispitivanih pasa, odmah posle kućnih grinja. Pozitivna reakcija zabeležena je kod 66% pasa iz grupe atopičara i kod 10% klinički zdravih pasa. Kod pasa koji su pokazali pozitivnu reakciju na intradermalnom testu na polen ambrozije, sekundarne lezije kože su se najčešće javljale na šapama i oko usana. Kod 88% ovih pasa na koži je u povećanom broju dijagnostikovana Malassezia pachydermatis, a kod 64% jedinki je registrovan otitis externa različite etiologije. Na osnovu dobijenih rezultata se može zaključiti da je alergen biljke Ambrosia artemisiifolia veoma značajan u indukciji atopijskog dermatitisa kod populacije pasa sa šireg područja Beograda.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis, Značaj alergena biljke Ambrosia artemisiifolia u etiopatogenezi atopijskog dermatitisa pasa",
volume = "60",
number = "2-3",
pages = "183-196",
doi = "10.2298/AVB1003183M"
}

Milčić-Matić, N., Popović, N., Lazarević, M.,& Medenica, L.. (2010). The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 183-196.
https://doi.org/10.2298/AVB1003183M

Milčić-Matić N, Popović N, Lazarević M, Medenica L. The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis. in Acta Veterinaria-Beograd. 2010;60(2-3):183-196.
doi:10.2298/AVB1003183M .

Milčić-Matić, Natalija, Popović, N., Lazarević, Miodrag, Medenica, Ljiljana, "The role of Ambrosia artemisiifolia allergen in canine atopic dermatitis" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):183-196,
https://doi.org/10.2298/AVB1003183M . .

TY  - JOUR
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Gledić, Dušan
AU  - Lazarević, Miodrag
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/725
AB  - The aim of this paper was to examine the effect of different duration sound stress on immune organs of broiler chickens of different age. Nine groups, with 10 chickens in each group were included in experiment. The histological structure of bursa of Fabricius, thymus, and spleen were analyzed. The results indicated that the bursa of Fabricius, in relation to the other examined organs, was the most sensitive to this kind of stress. Histological changes of spleen and thymus were also observed, but less prominent except in chickens after more than 30 days of exposure to stress. According to our results, degree of histological changes of immune organs under the influence of sound stress depends on the length of exposure and age of chickens.
AB  - Cilj rada je da se ispita uticaj zvučnog stresora na imunološke organe brojlera koji su bili izlagani u različitim uzrastima i različitom trajanju stresora. Formirano je devet grupa, a svaka grupa je imala po 10 pilića. Ispitivana je histološka građa Fabricijeve burze, grudne žlezde i slezine. Rezultati ukazuju da je Fabricijeva burza, u odnosu na ostale ispitivane organe, najosetljivija na delovanje ove vrste stresora. Promene se uočavaju i kod grudne žlezde i slezine, ali su manje i javljaju se nakon izlaganja stresoru više od 30 dana. Stepen promena u histološkoj građi imunoloških organa pod uticajem zvučnog stresora zavisi od dužine izlaganja kao i uzrasta pilića.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - The influence of long term sound stress on histological structure of immune organs in broiler chickens
T1  - Uticaj dugotrajnog zvučnog stresa na histološku građu imunoloških organa kod brojlerskih pilića
IS  - 118
SP  - 151
EP  - 159
DO  - 10.2298/ZMSPN1018151Z
ER  - 

@article{
author = "Žikić, Dragan and Ušćebrka, Gordana and Gledić, Dušan and Lazarević, Miodrag",
year = "2010",
abstract = "The aim of this paper was to examine the effect of different duration sound stress on immune organs of broiler chickens of different age. Nine groups, with 10 chickens in each group were included in experiment. The histological structure of bursa of Fabricius, thymus, and spleen were analyzed. The results indicated that the bursa of Fabricius, in relation to the other examined organs, was the most sensitive to this kind of stress. Histological changes of spleen and thymus were also observed, but less prominent except in chickens after more than 30 days of exposure to stress. According to our results, degree of histological changes of immune organs under the influence of sound stress depends on the length of exposure and age of chickens., Cilj rada je da se ispita uticaj zvučnog stresora na imunološke organe brojlera koji su bili izlagani u različitim uzrastima i različitom trajanju stresora. Formirano je devet grupa, a svaka grupa je imala po 10 pilića. Ispitivana je histološka građa Fabricijeve burze, grudne žlezde i slezine. Rezultati ukazuju da je Fabricijeva burza, u odnosu na ostale ispitivane organe, najosetljivija na delovanje ove vrste stresora. Promene se uočavaju i kod grudne žlezde i slezine, ali su manje i javljaju se nakon izlaganja stresoru više od 30 dana. Stepen promena u histološkoj građi imunoloških organa pod uticajem zvučnog stresora zavisi od dužine izlaganja kao i uzrasta pilića.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "The influence of long term sound stress on histological structure of immune organs in broiler chickens, Uticaj dugotrajnog zvučnog stresa na histološku građu imunoloških organa kod brojlerskih pilića",
number = "118",
pages = "151-159",
doi = "10.2298/ZMSPN1018151Z"
}

Žikić, D., Ušćebrka, G., Gledić, D.,& Lazarević, M.. (2010). The influence of long term sound stress on histological structure of immune organs in broiler chickens. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(118), 151-159.
https://doi.org/10.2298/ZMSPN1018151Z

Žikić D, Ušćebrka G, Gledić D, Lazarević M. The influence of long term sound stress on histological structure of immune organs in broiler chickens. in Zbornik Matice srpske za prirodne nauke. 2010;(118):151-159.
doi:10.2298/ZMSPN1018151Z .

Žikić, Dragan, Ušćebrka, Gordana, Gledić, Dušan, Lazarević, Miodrag, "The influence of long term sound stress on histological structure of immune organs in broiler chickens" in Zbornik Matice srpske za prirodne nauke, no. 118 (2010):151-159,
https://doi.org/10.2298/ZMSPN1018151Z . .