TY  - JOUR
AU  - Vidaković Knežević, Suzana
AU  - Knežević, Slobodan
AU  - Vranešević, Jelena
AU  - Milanov, Dubravka
AU  - Ružić, Zoran
AU  - Karabasil, Nedjeljko
AU  - Kocić-Tanackov, Sunčica
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3875
AB  - Yersiniosis, one of the leading foodborne infections in the European Union, is caused by
Yersinia enterocolitica. In this study, the antibacterial and antibiofilm effects of cinnamon (Cinnamomum
zeylanicum Nees), clove (Syzygium aromaticum L.), oregano (Origanum vulgare L.), rosemary (Rosmarinus
officinalis L.), thyme (Thymus vulgaris L.), and winter savory (Satureja montana L.) essential oils were
investigated against Y. enterocolitica strains belonging to the bioserotype 4/O:3. Cinnamon essential
oil showed the highest antibacterial activity, with an MIC value 0.09 µL/mL, followed by oregano
and thyme essential oils, with MIC values from 0.09 to 0.18 µL/mL, and from 0.18 to 0.23 µL/mL,
respectively. Thyme essential oil at 0.23 µL/g (MIC) and at 0.46 µL/g (2MIC) significantly (p < 0.05)
reduced the number of Y. enterocolitica by 0.38 log CFU/g and 0.64 log CFU/g, respectively, in minced
pork meat during storage at 4 ◦C for 4 days. The Y. enterocolitica strains formed biofilms at 15 ◦C and
37 ◦C in tryptic soy broth and Luria–Bertani broth, while no biofilms were obtained at 5 ◦C, and in
meat broth nutrient media. Applying the minimum bactericidal concentrations of cinnamon, clove,
oregano, rosemary, thyme, and winter savory essential oils on preformed biofilms led to significant
reductions being observed in the range from 45.34% to 78.89%. A scanning electron microscopy
assay showed the devastating impact of oregano and thyme essential oils on the morphology of Y.
enterocolitica bacterial cells. In conclusion, the results of this study show that essential oils possess
high anti-Yersinia and antibiofilm effects.
PB  - MDPI
T2  - Foods
T1  - Using essential oils to reduce Yersinia enterocolitica in minced meat and in biofilms
VL  - 13
IS  - 5
SP  - 806
DO  - 10.3390/foods13050806
ER  - 

@article{
author = "Vidaković Knežević, Suzana and Knežević, Slobodan and Vranešević, Jelena and Milanov, Dubravka and Ružić, Zoran and Karabasil, Nedjeljko and Kocić-Tanackov, Sunčica",
year = "2024",
abstract = "Yersiniosis, one of the leading foodborne infections in the European Union, is caused by
Yersinia enterocolitica. In this study, the antibacterial and antibiofilm effects of cinnamon (Cinnamomum
zeylanicum Nees), clove (Syzygium aromaticum L.), oregano (Origanum vulgare L.), rosemary (Rosmarinus
officinalis L.), thyme (Thymus vulgaris L.), and winter savory (Satureja montana L.) essential oils were
investigated against Y. enterocolitica strains belonging to the bioserotype 4/O:3. Cinnamon essential
oil showed the highest antibacterial activity, with an MIC value 0.09 µL/mL, followed by oregano
and thyme essential oils, with MIC values from 0.09 to 0.18 µL/mL, and from 0.18 to 0.23 µL/mL,
respectively. Thyme essential oil at 0.23 µL/g (MIC) and at 0.46 µL/g (2MIC) significantly (p < 0.05)
reduced the number of Y. enterocolitica by 0.38 log CFU/g and 0.64 log CFU/g, respectively, in minced
pork meat during storage at 4 ◦C for 4 days. The Y. enterocolitica strains formed biofilms at 15 ◦C and
37 ◦C in tryptic soy broth and Luria–Bertani broth, while no biofilms were obtained at 5 ◦C, and in
meat broth nutrient media. Applying the minimum bactericidal concentrations of cinnamon, clove,
oregano, rosemary, thyme, and winter savory essential oils on preformed biofilms led to significant
reductions being observed in the range from 45.34% to 78.89%. A scanning electron microscopy
assay showed the devastating impact of oregano and thyme essential oils on the morphology of Y.
enterocolitica bacterial cells. In conclusion, the results of this study show that essential oils possess
high anti-Yersinia and antibiofilm effects.",
publisher = "MDPI",
journal = "Foods",
title = "Using essential oils to reduce Yersinia enterocolitica in minced meat and in biofilms",
volume = "13",
number = "5",
pages = "806",
doi = "10.3390/foods13050806"
}

Vidaković Knežević, S., Knežević, S., Vranešević, J., Milanov, D., Ružić, Z., Karabasil, N.,& Kocić-Tanackov, S.. (2024). Using essential oils to reduce Yersinia enterocolitica in minced meat and in biofilms. in Foods
MDPI., 13(5), 806.
https://doi.org/10.3390/foods13050806

Vidaković Knežević S, Knežević S, Vranešević J, Milanov D, Ružić Z, Karabasil N, Kocić-Tanackov S. Using essential oils to reduce Yersinia enterocolitica in minced meat and in biofilms. in Foods. 2024;13(5):806.
doi:10.3390/foods13050806 .

Vidaković Knežević, Suzana, Knežević, Slobodan, Vranešević, Jelena, Milanov, Dubravka, Ružić, Zoran, Karabasil, Nedjeljko, Kocić-Tanackov, Sunčica, "Using essential oils to reduce Yersinia enterocolitica in minced meat and in biofilms" in Foods, 13, no. 5 (2024):806,
https://doi.org/10.3390/foods13050806 . .

TY  - CONF
AU  - Radalj, Andrea
AU  - Stevanović, Oliver
AU  - Milanov, Dubravka
AU  - Prošić, Isidora
AU  - Gajdov, Vladimir
AU  - Milić, Nenad
AU  - Sladojević, Željko
AU  - Nedić, Drago
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3880
AB  - Nocardia cyriacigeorgica је узрочник који се све чешће изолује у случајевима нокардиозе људи, међутим његова идентификација до нивоа врсте представља изазов за већину микробиолошких лабораторија. Инфекције изазване овом актиномицетом се релативно ретко сусрећу у ветеринарској клиничкој пракси при чему доступни литературни подаци обухватају мали број описаних случајева пиогрануломатозних лезија код животиња. Приказана студија описује случај тешког маститиса говеда на малом газдинству у Босни и Херцеговини изазваног узрочником N. cyriacigeorgica који је безуспешно третиран применом стандардне антибиотске терапије. С обзиром да изолат није идентификован применом конвенционалних микробиолошких техника, извршена је MALDI-ToF MS анализа чији су резултати били недовољно поуздани. У циљу утврђивања врсте анализирана је секвенца гена 16S субјединице рибозомалне рибонуклеинске киселине (16S rRNK). Наведени налаз је додатно потврђен применом технике секвенцирања више генских локуса (MLST) при чему су коришћене секвенце gyrB, 16S rRNK, secA1 и hsp65. Метода диск дифузије за испитивање осетљивости изолата N. cyriacigeorgica на антибиотике је примењена у складу са препорученим публикованим упутствима. Колико је ауторима познато, ово представља први случај маститиса говеда изазван узрочником N. cyriacigeorgica у Европи, али и први изолат Nocardia spp. пореклом од животиња за чију је идентификацију примењен MLST.
AB  - Although Nocardia cyriacigeorgica is recognized as a cause of human nocardiosis and is regarded as an emerging pathogen, many clinical laboratories find it difficult to identify this organism at the species level. The infections caused by this actinomycete are relatively uncommon in veterinary clinical practice, and the limited information currently available mostly relates to several cases of pyogranulomatous lesions in animals. We describe a case of severe bovine mastitis in a small holding in Bosnia and Herzegovina caused by N. cyriacigeorgica that did not improve after standard antibiotic therapy. The causative agent could not be identified using standard microbiological techniques and MALDI-ToF MS analysis was performed, yielding unclear results. Subsequently, the species N. cyriacigeorgica was confirmed by 16S rRNA sequence analysis, which was further validated by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences. The disc diffusion method was used to test for antibiotic susceptibility in accordance with published guidelines. To the best of our knowledge, this is the first report of N. cyriacigeorgica isolation from a clinical case of bovine mastitis in Europe, as well as the first time a strain of Nocardia spp. isolated from animals has been distinguished using the MLSA method.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Nocardia cyriacigeorgica kao uzročnik teškog slučaja mastitisa goveda
T1  - A Nocardia cyriacigeorgica strain causing severe bovine mastitis
SP  - 190
EP  - 191
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3880
ER  - 

@conference{
author = "Radalj, Andrea and Stevanović, Oliver and Milanov, Dubravka and Prošić, Isidora and Gajdov, Vladimir and Milić, Nenad and Sladojević, Željko and Nedić, Drago",
year = "2024",
abstract = "Nocardia cyriacigeorgica је узрочник који се све чешће изолује у случајевима нокардиозе људи, међутим његова идентификација до нивоа врсте представља изазов за већину микробиолошких лабораторија. Инфекције изазване овом актиномицетом се релативно ретко сусрећу у ветеринарској клиничкој пракси при чему доступни литературни подаци обухватају мали број описаних случајева пиогрануломатозних лезија код животиња. Приказана студија описује случај тешког маститиса говеда на малом газдинству у Босни и Херцеговини изазваног узрочником N. cyriacigeorgica који је безуспешно третиран применом стандардне антибиотске терапије. С обзиром да изолат није идентификован применом конвенционалних микробиолошких техника, извршена је MALDI-ToF MS анализа чији су резултати били недовољно поуздани. У циљу утврђивања врсте анализирана је секвенца гена 16S субјединице рибозомалне рибонуклеинске киселине (16S rRNK). Наведени налаз је додатно потврђен применом технике секвенцирања више генских локуса (MLST) при чему су коришћене секвенце gyrB, 16S rRNK, secA1 и hsp65. Метода диск дифузије за испитивање осетљивости изолата N. cyriacigeorgica на антибиотике је примењена у складу са препорученим публикованим упутствима. Колико је ауторима познато, ово представља први случај маститиса говеда изазван узрочником N. cyriacigeorgica у Европи, али и први изолат Nocardia spp. пореклом од животиња за чију је идентификацију примењен MLST., Although Nocardia cyriacigeorgica is recognized as a cause of human nocardiosis and is regarded as an emerging pathogen, many clinical laboratories find it difficult to identify this organism at the species level. The infections caused by this actinomycete are relatively uncommon in veterinary clinical practice, and the limited information currently available mostly relates to several cases of pyogranulomatous lesions in animals. We describe a case of severe bovine mastitis in a small holding in Bosnia and Herzegovina caused by N. cyriacigeorgica that did not improve after standard antibiotic therapy. The causative agent could not be identified using standard microbiological techniques and MALDI-ToF MS analysis was performed, yielding unclear results. Subsequently, the species N. cyriacigeorgica was confirmed by 16S rRNA sequence analysis, which was further validated by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences. The disc diffusion method was used to test for antibiotic susceptibility in accordance with published guidelines. To the best of our knowledge, this is the first report of N. cyriacigeorgica isolation from a clinical case of bovine mastitis in Europe, as well as the first time a strain of Nocardia spp. isolated from animals has been distinguished using the MLSA method.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Nocardia cyriacigeorgica kao uzročnik teškog slučaja mastitisa goveda, A Nocardia cyriacigeorgica strain causing severe bovine mastitis",
pages = "190-191",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3880"
}

Radalj, A., Stevanović, O., Milanov, D., Prošić, I., Gajdov, V., Milić, N., Sladojević, Ž.,& Nedić, D.. (2024). Nocardia cyriacigeorgica kao uzročnik teškog slučaja mastitisa goveda. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 190-191.
https://hdl.handle.net/21.15107/rcub_veterinar_3880

Radalj A, Stevanović O, Milanov D, Prošić I, Gajdov V, Milić N, Sladojević Ž, Nedić D. Nocardia cyriacigeorgica kao uzročnik teškog slučaja mastitisa goveda. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:190-191.
https://hdl.handle.net/21.15107/rcub_veterinar_3880 .

Radalj, Andrea, Stevanović, Oliver, Milanov, Dubravka, Prošić, Isidora, Gajdov, Vladimir, Milić, Nenad, Sladojević, Željko, Nedić, Drago, "Nocardia cyriacigeorgica kao uzročnik teškog slučaja mastitisa goveda" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):190-191,
https://hdl.handle.net/21.15107/rcub_veterinar_3880 .

TY  - CONF
AU  - Vidaković Knežević, Suzana
AU  - Vranešević, Jelena
AU  - Knežević, Slobodan
AU  - Ružić, Zoran
AU  - Milanov, Dubravka
AU  - Kocić-Tanackov, Sunčica
AU  - Karabasil, Nedjeljko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3082
AB  - Meso je nutritivno vredna namirnica, zbog čega je sklona mikrobiološkoj
kontaminaciji i kvaru. Glavne hranom prenosive patogene bakterije, kao što su
Salmonella spp., Listeria monocytogenes i Yersinia enterocolitica, često povezuju sa
mesom. Ovi uzročnici se nalaze na vrhu liste prijavljenih zoonoza kod ljudi. Kako
bi se povećao mikrobiološki kvalitet mesa poslednjih decenija su u upotrebi
alternativne supstance, između ostalog i etarska ulja, jedinjenja dobijena
ekstrakcijom iz biljaka, koje čine “bezbednu” alternativu hemijskim i sintetskim
aditivima, u cilju postizanja antimikrobnih i antioksidativnih efekata. Mnoga
istraživanja prikazuju antibakterijsko dejstvo etarskih ulja. Efekat delovanja
etarskih ulja na bakterije ogleda se u inhibiciji njihovog rasta (bakteriostatski
efekat) ili njihovoj destrukciji (baktericidni efekat). U ovom radu prikazana su
komercijalno dostupna etarska ulja prepoznata kao bezbedna za prehrambenu
upotrebu, njihova antibakterijska dejstva protiv hranom prenosivih bakterija u in
vitro uslovima, mehanizmi delovanja ovih etarskih ulja na Gram pozitivne i Gram
negativne bakterije, kao i njihova primena na modelu mesa.
AB  - Meat is rich in nutrients and thus highly prone to microbial contamination. Main
foodborne pathogens, including Salmonella spp., Listeria monocytogenes, and
Yersinia enterocolitica, are associated with meat. Those agents are on top of the list
as the most reported zoonoses in humans. In order to increase the microbial
quality of meat, alternative substances are applied. This includes essential oils,
compounds extracted from plants, as a “safe” alternative to chemical or synthetic
antimicrobials and antioxidants. Many studies have demonstrated the
antibacterial activity of essential oils. The effect of essential oils on bacteria is
reflected in the inhibition of their growth (bacteriostatic effect) or the destruction
of the bacterial cell (bactericidal effect). This paper reviews commercially
available essential oils recognized as safe, in vitro antibacterial activity of essential
oils against foodborne pathogens, mechanisms of action of essential oils on Gram
positive and Gram negative bacteria, and application of essential oils in model
meat system.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Efekat etarskih ulja na patogene bakterije poreklom iz mesa
T1  - The effect of essential oils against foodborne pathogens associated with meat
SP  - 144
EP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3082
ER  - 

@conference{
author = "Vidaković Knežević, Suzana and Vranešević, Jelena and Knežević, Slobodan and Ružić, Zoran and Milanov, Dubravka and Kocić-Tanackov, Sunčica and Karabasil, Nedjeljko",
year = "2023",
abstract = "Meso je nutritivno vredna namirnica, zbog čega je sklona mikrobiološkoj
kontaminaciji i kvaru. Glavne hranom prenosive patogene bakterije, kao što su
Salmonella spp., Listeria monocytogenes i Yersinia enterocolitica, često povezuju sa
mesom. Ovi uzročnici se nalaze na vrhu liste prijavljenih zoonoza kod ljudi. Kako
bi se povećao mikrobiološki kvalitet mesa poslednjih decenija su u upotrebi
alternativne supstance, između ostalog i etarska ulja, jedinjenja dobijena
ekstrakcijom iz biljaka, koje čine “bezbednu” alternativu hemijskim i sintetskim
aditivima, u cilju postizanja antimikrobnih i antioksidativnih efekata. Mnoga
istraživanja prikazuju antibakterijsko dejstvo etarskih ulja. Efekat delovanja
etarskih ulja na bakterije ogleda se u inhibiciji njihovog rasta (bakteriostatski
efekat) ili njihovoj destrukciji (baktericidni efekat). U ovom radu prikazana su
komercijalno dostupna etarska ulja prepoznata kao bezbedna za prehrambenu
upotrebu, njihova antibakterijska dejstva protiv hranom prenosivih bakterija u in
vitro uslovima, mehanizmi delovanja ovih etarskih ulja na Gram pozitivne i Gram
negativne bakterije, kao i njihova primena na modelu mesa., Meat is rich in nutrients and thus highly prone to microbial contamination. Main
foodborne pathogens, including Salmonella spp., Listeria monocytogenes, and
Yersinia enterocolitica, are associated with meat. Those agents are on top of the list
as the most reported zoonoses in humans. In order to increase the microbial
quality of meat, alternative substances are applied. This includes essential oils,
compounds extracted from plants, as a “safe” alternative to chemical or synthetic
antimicrobials and antioxidants. Many studies have demonstrated the
antibacterial activity of essential oils. The effect of essential oils on bacteria is
reflected in the inhibition of their growth (bacteriostatic effect) or the destruction
of the bacterial cell (bactericidal effect). This paper reviews commercially
available essential oils recognized as safe, in vitro antibacterial activity of essential
oils against foodborne pathogens, mechanisms of action of essential oils on Gram
positive and Gram negative bacteria, and application of essential oils in model
meat system.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Efekat etarskih ulja na patogene bakterije poreklom iz mesa, The effect of essential oils against foodborne pathogens associated with meat",
pages = "144-145",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3082"
}

Vidaković Knežević, S., Vranešević, J., Knežević, S., Ružić, Z., Milanov, D., Kocić-Tanackov, S.,& Karabasil, N.. (2023). Efekat etarskih ulja na patogene bakterije poreklom iz mesa. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 144-145.
https://hdl.handle.net/21.15107/rcub_veterinar_3082

Vidaković Knežević S, Vranešević J, Knežević S, Ružić Z, Milanov D, Kocić-Tanackov S, Karabasil N. Efekat etarskih ulja na patogene bakterije poreklom iz mesa. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:144-145.
https://hdl.handle.net/21.15107/rcub_veterinar_3082 .

Vidaković Knežević, Suzana, Vranešević, Jelena, Knežević, Slobodan, Ružić, Zoran, Milanov, Dubravka, Kocić-Tanackov, Sunčica, Karabasil, Nedjeljko, "Efekat etarskih ulja na patogene bakterije poreklom iz mesa" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):144-145,
https://hdl.handle.net/21.15107/rcub_veterinar_3082 .

TY  - JOUR
AU  - Stevanović, Oliver
AU  - Milanov, Dubravka
AU  - Đilas, Milan
AU  - Aleksić, Nevenka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2463
AB  - Highly mucoid Streptococcus uberis were isolated on blood agar from five milk samples originating from two dairy cow farms. All the isolates were CAMP test and esculin hydrolysis negative. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) following prior extraction with 70% formic acid was used for identification to the species level. Given that it is plausible that such strains could be isolated in the future from dairy cows by laboratories in Serbia and the Balkans, we consider this case report to be a useful instruction for veterinary bacteriology laboratories.
PB  - Faculty of Veterinary Medicine, University of Belgrade
T2  - Veterinarski Glasnik
T1  - Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification
T1  - Mukoidni izolati  Streptococcus uberis  iz mleka  krava sa mastitisom– poteškoće u identifikaciji
VL  - 76
IS  - 1
DO  - 10.2298/VETGL210929003S
ER  - 

@article{
author = "Stevanović, Oliver and Milanov, Dubravka and Đilas, Milan and Aleksić, Nevenka",
year = "2022",
abstract = "Highly mucoid Streptococcus uberis were isolated on blood agar from five milk samples originating from two dairy cow farms. All the isolates were CAMP test and esculin hydrolysis negative. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) following prior extraction with 70% formic acid was used for identification to the species level. Given that it is plausible that such strains could be isolated in the future from dairy cows by laboratories in Serbia and the Balkans, we consider this case report to be a useful instruction for veterinary bacteriology laboratories.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Veterinarski Glasnik",
title = "Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification, Mukoidni izolati  Streptococcus uberis  iz mleka  krava sa mastitisom– poteškoće u identifikaciji",
volume = "76",
number = "1",
doi = "10.2298/VETGL210929003S"
}

Stevanović, O., Milanov, D., Đilas, M.,& Aleksić, N.. (2022). Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification. in Veterinarski Glasnik
Faculty of Veterinary Medicine, University of Belgrade., 76(1).
https://doi.org/10.2298/VETGL210929003S

Stevanović O, Milanov D, Đilas M, Aleksić N. Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification. in Veterinarski Glasnik. 2022;76(1).
doi:10.2298/VETGL210929003S .

Stevanović, Oliver, Milanov, Dubravka, Đilas, Milan, Aleksić, Nevenka, "Mucoid bovine mastitis isolates of Streptococcus uberis – difficulties in identification" in Veterinarski Glasnik, 76, no. 1 (2022),
https://doi.org/10.2298/VETGL210929003S . .

TY  - JOUR
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2831
AB  - It has been established that some clones of pathogenic bacteria such as Salmonella spp., Escherichia coli ST131, and methicillin-resistant Staphylococcus aureus (MRSA) tend to spread worldwide. Th erefore, epidemiological surveys have been conducted to identify the source of infection and to break the chain of infection. In this article, it was pointed out that common international clones of Salmonella are represented with the serotypes Typhimurium, Kentucky, Infantis and Enteritidis. Serovars Typhimurium and Kentucky display multidrug-resistant phenotypes more frequently. Several sequence types of E. coli and the international clone ST131 are described, including clades C1 and C2 with the extended-spectrum cephalosporinresistance genes (blaCTX-M-15 or blaCTX-M-27). Th ese pathogens are oft en found in both humans and animals. It is noted that Staphylococcus aureus became resistant to methicillin almost instantly aft er its introduction into clinical practice. Soon aft erwards, MRSA found its way to farm animals and wildlife. Th e cycles of infection are bidirectional: humans can disseminate MRSA in the environment but animals may also be sources of infection for humans. Comprehensive work has been done by epidemiologists to introduce all necessary measures to eliminate MRSA from hospitals. Also, much eff ort has been made in MRSA control to prevent infections on animal farms and contamination in the primary food production chain. As the struggle with pathogenic bacteria continues, we face the incessant threat of new resistance and virulence mechanisms, which bacteria use to resist the hostile environment and enhance their survival in their natural habitats including humans and animals. Th erefore, the capacity of certain bacteria to spread due to their virulence mechanisms and resistance phenotypes is presented, and a brief description of the research conducted in Serbia is included.
PB  - Novi sad : Scientific Veterinary Institute
T2  - Archives of Veterinary Medicine
T1  - The global spread and epidemiological characteristics of Salmonella spp., Escherichia coli and Methicillin - resistant Staphylococcus aureus (MRSA): related researach studies in Serbia
VL  - 15
IS  - 1
SP  - 55
EP  - 67
DO  - 10.46784/eavm.v15i1.294
ER  - 

@article{
author = "Velhner, Maja and Milanov, Dubravka and Aleksić, Nevenka",
year = "2022",
abstract = "It has been established that some clones of pathogenic bacteria such as Salmonella spp., Escherichia coli ST131, and methicillin-resistant Staphylococcus aureus (MRSA) tend to spread worldwide. Th erefore, epidemiological surveys have been conducted to identify the source of infection and to break the chain of infection. In this article, it was pointed out that common international clones of Salmonella are represented with the serotypes Typhimurium, Kentucky, Infantis and Enteritidis. Serovars Typhimurium and Kentucky display multidrug-resistant phenotypes more frequently. Several sequence types of E. coli and the international clone ST131 are described, including clades C1 and C2 with the extended-spectrum cephalosporinresistance genes (blaCTX-M-15 or blaCTX-M-27). Th ese pathogens are oft en found in both humans and animals. It is noted that Staphylococcus aureus became resistant to methicillin almost instantly aft er its introduction into clinical practice. Soon aft erwards, MRSA found its way to farm animals and wildlife. Th e cycles of infection are bidirectional: humans can disseminate MRSA in the environment but animals may also be sources of infection for humans. Comprehensive work has been done by epidemiologists to introduce all necessary measures to eliminate MRSA from hospitals. Also, much eff ort has been made in MRSA control to prevent infections on animal farms and contamination in the primary food production chain. As the struggle with pathogenic bacteria continues, we face the incessant threat of new resistance and virulence mechanisms, which bacteria use to resist the hostile environment and enhance their survival in their natural habitats including humans and animals. Th erefore, the capacity of certain bacteria to spread due to their virulence mechanisms and resistance phenotypes is presented, and a brief description of the research conducted in Serbia is included.",
publisher = "Novi sad : Scientific Veterinary Institute",
journal = "Archives of Veterinary Medicine",
title = "The global spread and epidemiological characteristics of Salmonella spp., Escherichia coli and Methicillin - resistant Staphylococcus aureus (MRSA): related researach studies in Serbia",
volume = "15",
number = "1",
pages = "55-67",
doi = "10.46784/eavm.v15i1.294"
}

Velhner, M., Milanov, D.,& Aleksić, N.. (2022). The global spread and epidemiological characteristics of Salmonella spp., Escherichia coli and Methicillin - resistant Staphylococcus aureus (MRSA): related researach studies in Serbia. in Archives of Veterinary Medicine
Novi sad : Scientific Veterinary Institute., 15(1), 55-67.
https://doi.org/10.46784/eavm.v15i1.294

Velhner M, Milanov D, Aleksić N. The global spread and epidemiological characteristics of Salmonella spp., Escherichia coli and Methicillin - resistant Staphylococcus aureus (MRSA): related researach studies in Serbia. in Archives of Veterinary Medicine. 2022;15(1):55-67.
doi:10.46784/eavm.v15i1.294 .

Velhner, Maja, Milanov, Dubravka, Aleksić, Nevenka, "The global spread and epidemiological characteristics of Salmonella spp., Escherichia coli and Methicillin - resistant Staphylococcus aureus (MRSA): related researach studies in Serbia" in Archives of Veterinary Medicine, 15, no. 1 (2022):55-67,
https://doi.org/10.46784/eavm.v15i1.294 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Knežević, Slobodan
AU  - Vidaković, Suzana
AU  - Pajić, Marko
AU  - Živkov-Baloš, Milica
AU  - Aleksić, Nevenka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1706
AB  - The results of the research into the microbiological contamination of litter used by broiler chickens are presented. Litter samples were taken prior to the introduction of chicks (day 0) and in 7-day intervals until the end of the fattening period. The total numbers of aerobic mesophilic microorganisms, yeasts, moulds and Clostridium perfringens spores, and the presence of bacteria of the Salmonella genus were determined. The total microbial count in newly laid litter was 7 log10 CFU/g, which increased to 9 log10 CFU/g by the 4th week. However, at the end of the 5th week, it was at the same level as in newly laid litter. C. perfringens spores, presumably originating from chicks' faeces, were first detected on day 7. In the next 7 days their number increased, reaching 3-4 log10 CFU/g, and remained at approximately same levels until the end of the research. The initial mould contamination was 5-6 log10 CFU/g. However, from day 21 moulds were not isolated, but only yeasts of the Saccharomyces genus. It is supposed that these were deposited with chicks' faeces, due to their presence in complete broiler feed. No bacteria of the Salmonella genus were ever isolated from the litter. In conclusion, the total numbers of microorganisms in deep litter reach their peak in approximately a month, which is followed by their decrease. Deep litter is a favourable environment for probiotic yeast cultures. Added to feed intended for broilers, they can positively influence the microbial composition of litter, providing healthier environment to fattening broilers.
AB  - U radu su prikazani rezultati ispitivanja mikrobiološke kontaminacije prostirke korišćene u tovu pilića. Prostirka je uzorkovana pre naseljavanja objekata i u nedeljnim intervalima tokom 35 dana tova pilića. Uzorci su ispitani na ukupan broj aerobnih mezofilnih mikroorganizama, kvasaca, plesni i spora Clostridium perfringens, kao i prisustvo bakterija roda Salmonella. Ukupan broj mikroorganizama u svežoj prostirci iznosio je 7 log10 CFU/g, a do 4 nedelje tova pilića povećao se do 9 log10 CFU/g. Međutim, na kraju pete nedelje tova, ukupan broj mikroorganizama u prostirci bio je na nivou vrednosti ustanovljenih u svežoj prostirci. Spore C. perfringens su ustanovljene tek sedmog dana od naseljavanja objekta, što ukazuje da u prostirku dospevaju fecesom pilića. Za 14 dana broj spora C. perfringens se povećao do 3-4 log10 CFU/g i na približno istim vrednostima zadržao do kraja ispitivanja. Sveža prostirka bila je kontaminirana plesnima u nivou od 5-6 log10 CFU/g, ali od 21. dana iz uzoraka prostirke nisu izolovane plesni, već samo kvasci roda Saccharomyces. Pretpostavka je da su kvasci u prostirku dospeli fecesom pilića, jer se koriste kao probiotske kulture u smešama za njihov tov. Bakterije roda Salmonella nisu izolovane iz prostirke. Rezultati ispitivanja pokazuju da ukupan broj mikroorganizama u prostirci dostiže svoj pik za oko mesec dana, nakon čega se smanjuje. Prostirka je pogodna sredina za život probiotskih kultura kvasaca i njihovo dodavanje u smeše za tov brojlera može imati povoljan uticaj na sastav mikroorganizama u prostirci, a time i obezbeđenje zdravije životne sredine u podnom sistemu uzgoja brojlera.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Microbial contamination of poultry litter during fattening period
T1  - Mikrobiološka kontaminacija prostirke tokom tova brojlera
VL  - 35
IS  - 3
SP  - 253
EP  - 265
DO  - 10.2298/BAH1903253M
ER  - 

@article{
author = "Milanov, Dubravka and Knežević, Slobodan and Vidaković, Suzana and Pajić, Marko and Živkov-Baloš, Milica and Aleksić, Nevenka",
year = "2019",
abstract = "The results of the research into the microbiological contamination of litter used by broiler chickens are presented. Litter samples were taken prior to the introduction of chicks (day 0) and in 7-day intervals until the end of the fattening period. The total numbers of aerobic mesophilic microorganisms, yeasts, moulds and Clostridium perfringens spores, and the presence of bacteria of the Salmonella genus were determined. The total microbial count in newly laid litter was 7 log10 CFU/g, which increased to 9 log10 CFU/g by the 4th week. However, at the end of the 5th week, it was at the same level as in newly laid litter. C. perfringens spores, presumably originating from chicks' faeces, were first detected on day 7. In the next 7 days their number increased, reaching 3-4 log10 CFU/g, and remained at approximately same levels until the end of the research. The initial mould contamination was 5-6 log10 CFU/g. However, from day 21 moulds were not isolated, but only yeasts of the Saccharomyces genus. It is supposed that these were deposited with chicks' faeces, due to their presence in complete broiler feed. No bacteria of the Salmonella genus were ever isolated from the litter. In conclusion, the total numbers of microorganisms in deep litter reach their peak in approximately a month, which is followed by their decrease. Deep litter is a favourable environment for probiotic yeast cultures. Added to feed intended for broilers, they can positively influence the microbial composition of litter, providing healthier environment to fattening broilers., U radu su prikazani rezultati ispitivanja mikrobiološke kontaminacije prostirke korišćene u tovu pilića. Prostirka je uzorkovana pre naseljavanja objekata i u nedeljnim intervalima tokom 35 dana tova pilića. Uzorci su ispitani na ukupan broj aerobnih mezofilnih mikroorganizama, kvasaca, plesni i spora Clostridium perfringens, kao i prisustvo bakterija roda Salmonella. Ukupan broj mikroorganizama u svežoj prostirci iznosio je 7 log10 CFU/g, a do 4 nedelje tova pilića povećao se do 9 log10 CFU/g. Međutim, na kraju pete nedelje tova, ukupan broj mikroorganizama u prostirci bio je na nivou vrednosti ustanovljenih u svežoj prostirci. Spore C. perfringens su ustanovljene tek sedmog dana od naseljavanja objekta, što ukazuje da u prostirku dospevaju fecesom pilića. Za 14 dana broj spora C. perfringens se povećao do 3-4 log10 CFU/g i na približno istim vrednostima zadržao do kraja ispitivanja. Sveža prostirka bila je kontaminirana plesnima u nivou od 5-6 log10 CFU/g, ali od 21. dana iz uzoraka prostirke nisu izolovane plesni, već samo kvasci roda Saccharomyces. Pretpostavka je da su kvasci u prostirku dospeli fecesom pilića, jer se koriste kao probiotske kulture u smešama za njihov tov. Bakterije roda Salmonella nisu izolovane iz prostirke. Rezultati ispitivanja pokazuju da ukupan broj mikroorganizama u prostirci dostiže svoj pik za oko mesec dana, nakon čega se smanjuje. Prostirka je pogodna sredina za život probiotskih kultura kvasaca i njihovo dodavanje u smeše za tov brojlera može imati povoljan uticaj na sastav mikroorganizama u prostirci, a time i obezbeđenje zdravije životne sredine u podnom sistemu uzgoja brojlera.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Microbial contamination of poultry litter during fattening period, Mikrobiološka kontaminacija prostirke tokom tova brojlera",
volume = "35",
number = "3",
pages = "253-265",
doi = "10.2298/BAH1903253M"
}

Milanov, D., Knežević, S., Vidaković, S., Pajić, M., Živkov-Baloš, M.,& Aleksić, N.. (2019). Microbial contamination of poultry litter during fattening period. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(3), 253-265.
https://doi.org/10.2298/BAH1903253M

Milanov D, Knežević S, Vidaković S, Pajić M, Živkov-Baloš M, Aleksić N. Microbial contamination of poultry litter during fattening period. in Biotechnology in Animal Husbandry. 2019;35(3):253-265.
doi:10.2298/BAH1903253M .

Milanov, Dubravka, Knežević, Slobodan, Vidaković, Suzana, Pajić, Marko, Živkov-Baloš, Milica, Aleksić, Nevenka, "Microbial contamination of poultry litter during fattening period" in Biotechnology in Animal Husbandry, 35, no. 3 (2019):253-265,
https://doi.org/10.2298/BAH1903253M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
AU  - Vidaković, Suzana
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1713
AB  - Commercial feed intended for dogs and cats is an almost unrecognised source of human infection with various serovars of Salmonella enterica. However, people may catch the infection both via direct contact with contaminated pet feed and by contact with pets, which usually shed Salmonella without signs of infection. A relatively new trend of feeding dogs and cats with raw feed is considered to be a special risk owing to the fact that it usually contains foodborne pathogens, such as Salmonella spp., Escherichia coli and Campylobacter spp. Nonetheless, the epidemiological data do not support this idea. In the current review relevant data on the significance of pet feed in the outbreak of human salmonellosis are discussed and the recommendations for the prevention of the infection originating from these sources are suggested.
AB  - Komercijalna hrana za pse i mačke je skoro nepoznat izvor infekcije ljudi bakterijama iz roda Salmonella. Ljudi se mogu inficirati direktnim kontaktom sa kontaminiranom hranom za kućne ljubimce ili kontaktom sa kućnim ljubimcima koji uobičajeno izlučuju salmonele bez kliničkih znakova infekcije. Iako se novi trend ishrane pasa i mačaka sirovom hranom sa tog aspekta razmatra kao poseban rizik (jer ova hrana uobičajeno sadrži hranom prenosive patogene kao što su Salmonella spp., Escherichia coli i Campylobacter spp.), epidemiološki podaci ne podržavaju ovakav stav. U ovom radu sumiramo relevatne podatke o značaju hrane za kućne ljubimce u pojavi salmoneloza ljudi, kao i preporuke za prevenciju pojave infekcija izazvanih salmonelama iz ovog izvora.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Salmonella spp. in pet feed and risk it poses to humans
T1  - Salmonella spp. u hrani za kućne ljubimce i rizik koji predstavlja za zdravlje ljudi
VL  - 46
IS  - 1
SP  - 137
EP  - 145
DO  - 10.5937/FFR1901137M
ER  - 

@article{
author = "Milanov, Dubravka and Aleksić, Nevenka and Vidaković, Suzana and Ljubojević, Dragana and Čabarkapa, Ivana",
year = "2019",
abstract = "Commercial feed intended for dogs and cats is an almost unrecognised source of human infection with various serovars of Salmonella enterica. However, people may catch the infection both via direct contact with contaminated pet feed and by contact with pets, which usually shed Salmonella without signs of infection. A relatively new trend of feeding dogs and cats with raw feed is considered to be a special risk owing to the fact that it usually contains foodborne pathogens, such as Salmonella spp., Escherichia coli and Campylobacter spp. Nonetheless, the epidemiological data do not support this idea. In the current review relevant data on the significance of pet feed in the outbreak of human salmonellosis are discussed and the recommendations for the prevention of the infection originating from these sources are suggested., Komercijalna hrana za pse i mačke je skoro nepoznat izvor infekcije ljudi bakterijama iz roda Salmonella. Ljudi se mogu inficirati direktnim kontaktom sa kontaminiranom hranom za kućne ljubimce ili kontaktom sa kućnim ljubimcima koji uobičajeno izlučuju salmonele bez kliničkih znakova infekcije. Iako se novi trend ishrane pasa i mačaka sirovom hranom sa tog aspekta razmatra kao poseban rizik (jer ova hrana uobičajeno sadrži hranom prenosive patogene kao što su Salmonella spp., Escherichia coli i Campylobacter spp.), epidemiološki podaci ne podržavaju ovakav stav. U ovom radu sumiramo relevatne podatke o značaju hrane za kućne ljubimce u pojavi salmoneloza ljudi, kao i preporuke za prevenciju pojave infekcija izazvanih salmonelama iz ovog izvora.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Salmonella spp. in pet feed and risk it poses to humans, Salmonella spp. u hrani za kućne ljubimce i rizik koji predstavlja za zdravlje ljudi",
volume = "46",
number = "1",
pages = "137-145",
doi = "10.5937/FFR1901137M"
}

Milanov, D., Aleksić, N., Vidaković, S., Ljubojević, D.,& Čabarkapa, I.. (2019). Salmonella spp. in pet feed and risk it poses to humans. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 46(1), 137-145.
https://doi.org/10.5937/FFR1901137M

Milanov D, Aleksić N, Vidaković S, Ljubojević D, Čabarkapa I. Salmonella spp. in pet feed and risk it poses to humans. in Food and Feed Research. 2019;46(1):137-145.
doi:10.5937/FFR1901137M .

Milanov, Dubravka, Aleksić, Nevenka, Vidaković, Suzana, Ljubojević, Dragana, Čabarkapa, Ivana, "Salmonella spp. in pet feed and risk it poses to humans" in Food and Feed Research, 46, no. 1 (2019):137-145,
https://doi.org/10.5937/FFR1901137M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Đilas, Milan
AU  - Velhner, Maja
AU  - Aleksić, Nevenka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2066
AB  - Although Clostridium tertium is supposed to be a foodborne pathogen, the data on its detection in foodstuff s is scarce, and there are no reports on its isolation from feed. In this communication paper, the isolation of C. tertium from a sample of soya semolina is described. C. tertium may be importantin diff erential diagnosis, when it is to be distinguished from Clostridium perfringens. It is a unique species due to the lack of key characteristics of the genus it belongs to because it grows in the presence of oxygen and does not produce toxins. It has been well-documented as a human pathogen, although its mechanisms of pathogenicity are still unknown. According to sporadic reports in veterinary medicine, it has been identifi ed as a rare causative agent of infections in cattle, pigs, birds and marine mammals.
AB  - Pretpostavka je da Clostridium tertium treba svrstati u patogene koji se
mogu preneti hranom, ali je malo podataka o njegovom nalazu u namirnicama, dok o izolaciji iz hrane za životinje nema dostupnih izveštaja. U
ovom saopštenju prikazujemo izolaciju C. tertium iz uzorka sojinog griza.
C. tertium može biti od značaja u diferencijalnoj dijagnostici kod izolacije
Clostridium perfringens. Specifi čna je vrsta jer ne poseduje ključne karakteristike roda kojem pripada: raste u prisustvu kiseonika i ne produkuje
toksine. Dobro je dokumentovan kao humani patogen, iako su mehanizmi
njegove virulencije i danas nepoznati. Na osnovu sporadičnih izveštaja u
veterinarskoj medicini, identifi kovan je retko kao uzročnik infekcija goveda, svinja, ptica i morskih sisara.
PB  - Scientific Veterinary Institute “Novi Sad”
T2  - Archives of Veterinary Medicine
T1  - Clostridium tertium isolated from feed
T1  - Izolacija clostridium tertium iz hrane za životinje
VL  - 12
IS  - 2
SP  - 5
EP  - 12
DO  - 10.46784/e-avm.v12i2.58
ER  - 

@article{
author = "Milanov, Dubravka and Đilas, Milan and Velhner, Maja and Aleksić, Nevenka",
year = "2019",
abstract = "Although Clostridium tertium is supposed to be a foodborne pathogen, the data on its detection in foodstuff s is scarce, and there are no reports on its isolation from feed. In this communication paper, the isolation of C. tertium from a sample of soya semolina is described. C. tertium may be importantin diff erential diagnosis, when it is to be distinguished from Clostridium perfringens. It is a unique species due to the lack of key characteristics of the genus it belongs to because it grows in the presence of oxygen and does not produce toxins. It has been well-documented as a human pathogen, although its mechanisms of pathogenicity are still unknown. According to sporadic reports in veterinary medicine, it has been identifi ed as a rare causative agent of infections in cattle, pigs, birds and marine mammals., Pretpostavka je da Clostridium tertium treba svrstati u patogene koji se
mogu preneti hranom, ali je malo podataka o njegovom nalazu u namirnicama, dok o izolaciji iz hrane za životinje nema dostupnih izveštaja. U
ovom saopštenju prikazujemo izolaciju C. tertium iz uzorka sojinog griza.
C. tertium može biti od značaja u diferencijalnoj dijagnostici kod izolacije
Clostridium perfringens. Specifi čna je vrsta jer ne poseduje ključne karakteristike roda kojem pripada: raste u prisustvu kiseonika i ne produkuje
toksine. Dobro je dokumentovan kao humani patogen, iako su mehanizmi
njegove virulencije i danas nepoznati. Na osnovu sporadičnih izveštaja u
veterinarskoj medicini, identifi kovan je retko kao uzročnik infekcija goveda, svinja, ptica i morskih sisara.",
publisher = "Scientific Veterinary Institute “Novi Sad”",
journal = "Archives of Veterinary Medicine",
title = "Clostridium tertium isolated from feed, Izolacija clostridium tertium iz hrane za životinje",
volume = "12",
number = "2",
pages = "5-12",
doi = "10.46784/e-avm.v12i2.58"
}

Milanov, D., Đilas, M., Velhner, M.,& Aleksić, N.. (2019). Clostridium tertium isolated from feed. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”., 12(2), 5-12.
https://doi.org/10.46784/e-avm.v12i2.58

Milanov D, Đilas M, Velhner M, Aleksić N. Clostridium tertium isolated from feed. in Archives of Veterinary Medicine. 2019;12(2):5-12.
doi:10.46784/e-avm.v12i2.58 .

Milanov, Dubravka, Đilas, Milan, Velhner, Maja, Aleksić, Nevenka, "Clostridium tertium isolated from feed" in Archives of Veterinary Medicine, 12, no. 2 (2019):5-12,
https://doi.org/10.46784/e-avm.v12i2.58 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
AU  - Baloš, Milica Živkov
AU  - Pajić, Marko
AU  - Knežević, Slobodan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2069
AB  - Necrotic enteritis (NE) or poultry clostridiosis is a disease which poses enormous health problems and makes tremendous economic losses to intensive poultry production worldwide. Despite having been targeted in extensive research for decades, a number of aspects of its pathogenesis remain unknown. For more than 30 years alfa-toxin has been considered to be the main virulence factor of the causative agent, but experimental research using a mutant Clostridium perfringens strain lacking the gene coding for this confirmed that alpha-toxin is not necessary for pathogenesis. Since the 1980s, NetB toxin has been the main suspected virulence factor. However, recently it has been discovered that the large clostridial cytotoxin named TpeL also contributes to the pathogenesis of NE. In spite of that, the prevalence of the genes which code for these toxins vary between the isolates of C. perfringens from the intestines of diseased poultry, which made clear that further investigation into their roles is necessary. It has been agreed that specific intestinal environmental conditions, which favour the growth and multiplication of C. perfringens, are key factors to the emergence of disease. Given that a battery of non-specific factors contributes to pathogenesis, as well as that it is impossible to eliminate them in intensive poultry production, not much hope remains that NE can be controlled. In this short review, the current knowledge on the pathogenesis of NE has been summarized.
AB  - Nekrotični  enteritis  (NE)  ili  klostridioza  živine,  predstavlja  veliki  zdravstveni  problem  i  nanosi  ogromne  ekonomske  gubitke  intenzivnoj  živinarskoj proizvodnji širom sveta. Brojni aspekti patogeneze NE su i danas nepoznati, uprkos tome što su decenijama predmet intenzivnih izučavanja. Više  od  30  godina  je  alfa-toksin  razmatran  kao  glavni  faktor  virulencije  uzročnika,  ali  su  eksperimentalna  istraživanja  primenom  mutantnog  soja  Clostridium perfringens, koji nema gen koji kodira sintezu alfa-toksina, pot-vrdila da ovaj toksin nije neophodan za nastanak bolesti. Od osamdesetih godina prošlog veka, NetB toksin je “glavni osumnjičeni” faktor virulencije, a od nedavno se smatra da i veliki klostridijalni ekstracelularni citotoksin, koji je nazvan TpeL, doprinosi patogenezi NE. Međutim, prevalencija gena koji kodiraju ove toksine, veoma varira kod izolata C. perfringens iz creva obolele  živine  i  jasno  je  da  su  neophodna  dodatna  ispitivanja  njihove  ul-oge.  Saglasnost  postoji  da  su  za  pojavu  bolesti  ključni  specifi  čni  uslovi  u  crevima koji pogoduju rastu i umnožavanju C. perfringens. Ako uzmemo u obzir da niz nespecifi čnih faktora tome doprinosi, kao i da ih je praktično nemoguće u potpunosti otkloniti u intenzivnoj živinarskoj proizvodnji, ne ostaje mnogo nade da je nekrotični enteritis moguće staviti pod kontrolu. U radu ukratko sumiramo trenutna stanovišta o patogenezi NE živine.
PB  - Scientific Veterinary Institute “Novi Sad”
T2  - Archives of Veterinary Medicine
T2  - Archives of Veterinary MedicineAVM
T1  - Currently unknown aspects of poultry necrotic enteritis pathogenesis
T1  - I danas nepoznati aspekti patogeneze nekrotičnog enteritisa živine
VL  - 12
IS  - 1
SP  - 55
EP  - 69
DO  - 10.46784/e-avm.v12i1.38
ER  - 

@article{
author = "Milanov, Dubravka and Aleksić, Nevenka and Baloš, Milica Živkov and Pajić, Marko and Knežević, Slobodan",
year = "2019",
abstract = "Necrotic enteritis (NE) or poultry clostridiosis is a disease which poses enormous health problems and makes tremendous economic losses to intensive poultry production worldwide. Despite having been targeted in extensive research for decades, a number of aspects of its pathogenesis remain unknown. For more than 30 years alfa-toxin has been considered to be the main virulence factor of the causative agent, but experimental research using a mutant Clostridium perfringens strain lacking the gene coding for this confirmed that alpha-toxin is not necessary for pathogenesis. Since the 1980s, NetB toxin has been the main suspected virulence factor. However, recently it has been discovered that the large clostridial cytotoxin named TpeL also contributes to the pathogenesis of NE. In spite of that, the prevalence of the genes which code for these toxins vary between the isolates of C. perfringens from the intestines of diseased poultry, which made clear that further investigation into their roles is necessary. It has been agreed that specific intestinal environmental conditions, which favour the growth and multiplication of C. perfringens, are key factors to the emergence of disease. Given that a battery of non-specific factors contributes to pathogenesis, as well as that it is impossible to eliminate them in intensive poultry production, not much hope remains that NE can be controlled. In this short review, the current knowledge on the pathogenesis of NE has been summarized., Nekrotični  enteritis  (NE)  ili  klostridioza  živine,  predstavlja  veliki  zdravstveni  problem  i  nanosi  ogromne  ekonomske  gubitke  intenzivnoj  živinarskoj proizvodnji širom sveta. Brojni aspekti patogeneze NE su i danas nepoznati, uprkos tome što su decenijama predmet intenzivnih izučavanja. Više  od  30  godina  je  alfa-toksin  razmatran  kao  glavni  faktor  virulencije  uzročnika,  ali  su  eksperimentalna  istraživanja  primenom  mutantnog  soja  Clostridium perfringens, koji nema gen koji kodira sintezu alfa-toksina, pot-vrdila da ovaj toksin nije neophodan za nastanak bolesti. Od osamdesetih godina prošlog veka, NetB toksin je “glavni osumnjičeni” faktor virulencije, a od nedavno se smatra da i veliki klostridijalni ekstracelularni citotoksin, koji je nazvan TpeL, doprinosi patogenezi NE. Međutim, prevalencija gena koji kodiraju ove toksine, veoma varira kod izolata C. perfringens iz creva obolele  živine  i  jasno  je  da  su  neophodna  dodatna  ispitivanja  njihove  ul-oge.  Saglasnost  postoji  da  su  za  pojavu  bolesti  ključni  specifi  čni  uslovi  u  crevima koji pogoduju rastu i umnožavanju C. perfringens. Ako uzmemo u obzir da niz nespecifi čnih faktora tome doprinosi, kao i da ih je praktično nemoguće u potpunosti otkloniti u intenzivnoj živinarskoj proizvodnji, ne ostaje mnogo nade da je nekrotični enteritis moguće staviti pod kontrolu. U radu ukratko sumiramo trenutna stanovišta o patogenezi NE živine.",
publisher = "Scientific Veterinary Institute “Novi Sad”",
journal = "Archives of Veterinary Medicine, Archives of Veterinary MedicineAVM",
title = "Currently unknown aspects of poultry necrotic enteritis pathogenesis, I danas nepoznati aspekti patogeneze nekrotičnog enteritisa živine",
volume = "12",
number = "1",
pages = "55-69",
doi = "10.46784/e-avm.v12i1.38"
}

Milanov, D., Aleksić, N., Baloš, M. Ž., Pajić, M.,& Knežević, S.. (2019). Currently unknown aspects of poultry necrotic enteritis pathogenesis. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”., 12(1), 55-69.
https://doi.org/10.46784/e-avm.v12i1.38

Milanov D, Aleksić N, Baloš MŽ, Pajić M, Knežević S. Currently unknown aspects of poultry necrotic enteritis pathogenesis. in Archives of Veterinary Medicine. 2019;12(1):55-69.
doi:10.46784/e-avm.v12i1.38 .

Milanov, Dubravka, Aleksić, Nevenka, Baloš, Milica Živkov, Pajić, Marko, Knežević, Slobodan, "Currently unknown aspects of poultry necrotic enteritis pathogenesis" in Archives of Veterinary Medicine, 12, no. 1 (2019):55-69,
https://doi.org/10.46784/e-avm.v12i1.38 . .

TY  - CONF
AU  - Lakićević, Brankica
AU  - Petrović, Zoran
AU  - Milanov, Dubravka
AU  - Zuber, Ivana
AU  - Janković, V.
AU  - Grković, Nevena
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1688
AB  - In this study, 20 Listeria monocytogenes isolates detected in a food processing environment and food products were tested for biofilm-forming ability in two different culture media: Tryptone Soya Broth and Luria Bertani Broth. Statistical analysis of the data obtained was performed with the MINITAB software package, version 16.0. The two-sample t-test and confidence interval were used for data analysis. Significant differences between the isolates were observed in the ability to form biofilms.
PB  - Iop Publishing Ltd, Bristol
C3  - 60th International Meat Industry Conference (MEATCON2019)
T1  - Influence of two different culture media on biofilm formation by Listeria monocytogenes isolated from a small-scale meat processing facility
VL  - 333
SP  - UNSP 012073
DO  - 10.1088/1755-1315/333/1/012073
ER  - 

@conference{
author = "Lakićević, Brankica and Petrović, Zoran and Milanov, Dubravka and Zuber, Ivana and Janković, V. and Grković, Nevena and Dimitrijević, Mirjana",
year = "2019",
abstract = "In this study, 20 Listeria monocytogenes isolates detected in a food processing environment and food products were tested for biofilm-forming ability in two different culture media: Tryptone Soya Broth and Luria Bertani Broth. Statistical analysis of the data obtained was performed with the MINITAB software package, version 16.0. The two-sample t-test and confidence interval were used for data analysis. Significant differences between the isolates were observed in the ability to form biofilms.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "60th International Meat Industry Conference (MEATCON2019)",
title = "Influence of two different culture media on biofilm formation by Listeria monocytogenes isolated from a small-scale meat processing facility",
volume = "333",
pages = "UNSP 012073",
doi = "10.1088/1755-1315/333/1/012073"
}

Lakićević, B., Petrović, Z., Milanov, D., Zuber, I., Janković, V., Grković, N.,& Dimitrijević, M.. (2019). Influence of two different culture media on biofilm formation by Listeria monocytogenes isolated from a small-scale meat processing facility. in 60th International Meat Industry Conference (MEATCON2019)
Iop Publishing Ltd, Bristol., 333, UNSP 012073.
https://doi.org/10.1088/1755-1315/333/1/012073

Lakićević B, Petrović Z, Milanov D, Zuber I, Janković V, Grković N, Dimitrijević M. Influence of two different culture media on biofilm formation by Listeria monocytogenes isolated from a small-scale meat processing facility. in 60th International Meat Industry Conference (MEATCON2019). 2019;333:UNSP 012073.
doi:10.1088/1755-1315/333/1/012073 .

Lakićević, Brankica, Petrović, Zoran, Milanov, Dubravka, Zuber, Ivana, Janković, V., Grković, Nevena, Dimitrijević, Mirjana, "Influence of two different culture media on biofilm formation by Listeria monocytogenes isolated from a small-scale meat processing facility" in 60th International Meat Industry Conference (MEATCON2019), 333 (2019):UNSP 012073,
https://doi.org/10.1088/1755-1315/333/1/012073 . .

TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1844
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .

TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1728
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Grego, Edita
AU  - Vidanović, Dejan
AU  - Milanov, Dubravka
AU  - Krnjaić, Dejan
AU  - Kehrenberg, Corinna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1648
AB  - The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
VL  - 24
IS  - 1
SP  - 95
EP  - 103
DO  - 10.1089/mdr.2017.0016
ER  - 

@article{
author = "Todorović, Dalibor and Velhner, Maja and Grego, Edita and Vidanović, Dejan and Milanov, Dubravka and Krnjaić, Dejan and Kehrenberg, Corinna",
year = "2018",
abstract = "The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia",
volume = "24",
number = "1",
pages = "95-103",
doi = "10.1089/mdr.2017.0016"
}

Todorović, D., Velhner, M., Grego, E., Vidanović, D., Milanov, D., Krnjaić, D.,& Kehrenberg, C.. (2018). Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 24(1), 95-103.
https://doi.org/10.1089/mdr.2017.0016

Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance. 2018;24(1):95-103.
doi:10.1089/mdr.2017.0016 .

Todorović, Dalibor, Velhner, Maja, Grego, Edita, Vidanović, Dejan, Milanov, Dubravka, Krnjaić, Dejan, Kehrenberg, Corinna, "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia" in Microbial Drug Resistance, 24, no. 1 (2018):95-103,
https://doi.org/10.1089/mdr.2017.0016 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Đilas, Milan
AU  - Velhner, Maja
AU  - Aleksić, Nevenka
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1563
AB  - In the present paper the laboratory isolation and identification of Bordatella bronchiseptica, the causative agent of canine tracheobronhitis, is described. A dog which suffered persistent cough, loss of appetite and fever was previously unsucceffully treated with antibiotics, which is why nasal swabs were taken and sent for microbiological assessment. The isolation of the causative agent was performed on routinely used standard solid growth mediums. The final identification of the isolate was done with MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and real-time PCR (polymerase chain reaction) assays. Therapy based on the results of the antibiogram lead to successful recovery. The necessity of cooperation of veterinary clinicians and veterinary microbiologists for timely and reliable identification of the microbe(s) and selection of antimicrobials based on the results of the susceptibility testing is emphasized. The significance of the collaboration between microbiological veterinary laboratories and those dealing with human material is underlined. These can provide precise identification of zoonotic agents.
AB  - U ovom radu prikazujemo laboratorijsku izolaciju i identifikaciju Bordatella bronchiseptica, uzročnika traheobronhitisa psa. Pas koji je imao uporan kašalj, ispoljavao gubitak apetita i imao hipertermiju, prethodno je bez uspeha bio lečen antibioticima, zbog čega su brisevi nosa poslati na mikrobiološki pregled. Izolacija uzročnika izvedena je na podlogama koje se uobičajeno koriste u mikrobiološkim laboratorijama. Identifikacija izolata do vrste izvedena je primenom MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) i real-time PCR (polymerase chain reaction) metoda. Posle primene terapije na osnovu antibiograma, pas je uspešno izlečen. Istaknuta je neophodnost saradnje veterinara kliničara i veterinarskih mikrobioloških laboratorija u cilju blagovremene i pouzdane identifikacije uzročnika i odabira terapije na osnovu rezultata ispitivanja osetljivosti izolata na antibiotike. Dodatno ukazujemo na značaj povezanosti mikrobioloških laboratorija humane i veterinarske medicine u preciznoj identifikaciji zoonotskih agenasa.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Laboratory diagnosis of Bordatella bronchiseptica tracheobronchitis in dog
T1  - Laboratorijska dijagnoza Tracheobronhitisa psa čiji je uzročnik Bordatella bronchiseptica
VL  - 11
IS  - 2
SP  - 33
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1563
ER  - 

@article{
author = "Milanov, Dubravka and Đilas, Milan and Velhner, Maja and Aleksić, Nevenka",
year = "2018",
abstract = "In the present paper the laboratory isolation and identification of Bordatella bronchiseptica, the causative agent of canine tracheobronhitis, is described. A dog which suffered persistent cough, loss of appetite and fever was previously unsucceffully treated with antibiotics, which is why nasal swabs were taken and sent for microbiological assessment. The isolation of the causative agent was performed on routinely used standard solid growth mediums. The final identification of the isolate was done with MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and real-time PCR (polymerase chain reaction) assays. Therapy based on the results of the antibiogram lead to successful recovery. The necessity of cooperation of veterinary clinicians and veterinary microbiologists for timely and reliable identification of the microbe(s) and selection of antimicrobials based on the results of the susceptibility testing is emphasized. The significance of the collaboration between microbiological veterinary laboratories and those dealing with human material is underlined. These can provide precise identification of zoonotic agents., U ovom radu prikazujemo laboratorijsku izolaciju i identifikaciju Bordatella bronchiseptica, uzročnika traheobronhitisa psa. Pas koji je imao uporan kašalj, ispoljavao gubitak apetita i imao hipertermiju, prethodno je bez uspeha bio lečen antibioticima, zbog čega su brisevi nosa poslati na mikrobiološki pregled. Izolacija uzročnika izvedena je na podlogama koje se uobičajeno koriste u mikrobiološkim laboratorijama. Identifikacija izolata do vrste izvedena je primenom MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) i real-time PCR (polymerase chain reaction) metoda. Posle primene terapije na osnovu antibiograma, pas je uspešno izlečen. Istaknuta je neophodnost saradnje veterinara kliničara i veterinarskih mikrobioloških laboratorija u cilju blagovremene i pouzdane identifikacije uzročnika i odabira terapije na osnovu rezultata ispitivanja osetljivosti izolata na antibiotike. Dodatno ukazujemo na značaj povezanosti mikrobioloških laboratorija humane i veterinarske medicine u preciznoj identifikaciji zoonotskih agenasa.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Laboratory diagnosis of Bordatella bronchiseptica tracheobronchitis in dog, Laboratorijska dijagnoza Tracheobronhitisa psa čiji je uzročnik Bordatella bronchiseptica",
volume = "11",
number = "2",
pages = "33-41",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1563"
}

Milanov, D., Đilas, M., Velhner, M.,& Aleksić, N.. (2018). Laboratory diagnosis of Bordatella bronchiseptica tracheobronchitis in dog. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 11(2), 33-41.
https://hdl.handle.net/21.15107/rcub_veterinar_1563

Milanov D, Đilas M, Velhner M, Aleksić N. Laboratory diagnosis of Bordatella bronchiseptica tracheobronchitis in dog. in Arhiv veterinarske medicine. 2018;11(2):33-41.
https://hdl.handle.net/21.15107/rcub_veterinar_1563 .

Milanov, Dubravka, Đilas, Milan, Velhner, Maja, Aleksić, Nevenka, "Laboratory diagnosis of Bordatella bronchiseptica tracheobronchitis in dog" in Arhiv veterinarske medicine, 11, no. 2 (2018):33-41,
https://hdl.handle.net/21.15107/rcub_veterinar_1563 .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Todorović, Dalibor
AU  - Aleksić, Nevenka
AU  - Čabarkapa, Ivana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1642
AB  - Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed.
AB  - Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals
T1  - Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja
VL  - 45
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/FFR1801067M
ER  - 

@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Todorović, Dalibor and Aleksić, Nevenka and Čabarkapa, Ivana",
year = "2018",
abstract = "Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed., Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals, Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja",
volume = "45",
number = "1",
pages = "67-76",
doi = "10.5937/FFR1801067M"
}

Milanov, D., Petrović, T., Todorović, D., Aleksić, N.,& Čabarkapa, I.. (2018). Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(1), 67-76.
https://doi.org/10.5937/FFR1801067M

Milanov D, Petrović T, Todorović D, Aleksić N, Čabarkapa I. Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research. 2018;45(1):67-76.
doi:10.5937/FFR1801067M .

Milanov, Dubravka, Petrović, Tamaš, Todorović, Dalibor, Aleksić, Nevenka, Čabarkapa, Ivana, "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals" in Food and Feed Research, 45, no. 1 (2018):67-76,
https://doi.org/10.5937/FFR1801067M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
AU  - Todorović, Dalibor
AU  - Bugarski, Dejan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1448
AB  - Pasteurella (P.) multocida is a heterogeneous species of Gram-negative bacteria which are common commensals of the upper respiratory system of various mammal and bird species, but are also opportunistic contagious zoonotic pathogens which cause a wide spectre of infections in domestic animals and humans. P. multocida is a rare cause of mastitis in dairy cows. The source of infection mainly remains unknown, mastitis usually is acute, and the therapy by intramammary administration of antibiotics does not lead to satisfactory results. Lethality is possible due to presence of endotoxins in blood. Literature data on P. multocida mastitis in dairy cows is particularly scarce, which is why such a case is described in the current work, with past medical history, clinical findings, laboratory diagnostics and therapeutic approach.
AB  - Pasteurella multocida je heterogena vrsta gram negativnih bakterija koje su uobičajeni komensali prednjih partija respiratornog sistema različitih vrsta sisara i ptica, ali i oportunistički, kontagiozni i zoonotski patogeni, koji izazivaju širok spektar infekcija kod domaćih životinja i ljudi. P. multocida spada u retke uzročnike mastitisa mlečnih krava. Izvor infekcije za mlečnu žlezdu uglavnom ostaje nepoznat, mastitis obično ima akutni tok, a terapija intramamarnom aplikacijom antibiotika ne daje zadovoljavajuće rezultate. Letalni ishod je moguć usled razvoja endotoksemije. U literaturi ima veoma malo podataka o mastitisima mlečnih krava čiji je uzročnik P. multocida, zbog čega u ovom radu opisujemo jedan takav slučaj, uz prikaz osnovnih anamnestičkih podataka, kliničke slike, laboratorijske dijagnostike i terapijskog pristupa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Pasteurella multocida mastitis in cow: Case report
T1  - Mastitis kod krave izazvan bakterijom Pasteurella multocida
VL  - 71
IS  - 2
SP  - 117
EP  - 122
DO  - 10.2298/VETGL170619011M
ER  - 

@article{
author = "Milanov, Dubravka and Aleksić, Nevenka and Todorović, Dalibor and Bugarski, Dejan",
year = "2017",
abstract = "Pasteurella (P.) multocida is a heterogeneous species of Gram-negative bacteria which are common commensals of the upper respiratory system of various mammal and bird species, but are also opportunistic contagious zoonotic pathogens which cause a wide spectre of infections in domestic animals and humans. P. multocida is a rare cause of mastitis in dairy cows. The source of infection mainly remains unknown, mastitis usually is acute, and the therapy by intramammary administration of antibiotics does not lead to satisfactory results. Lethality is possible due to presence of endotoxins in blood. Literature data on P. multocida mastitis in dairy cows is particularly scarce, which is why such a case is described in the current work, with past medical history, clinical findings, laboratory diagnostics and therapeutic approach., Pasteurella multocida je heterogena vrsta gram negativnih bakterija koje su uobičajeni komensali prednjih partija respiratornog sistema različitih vrsta sisara i ptica, ali i oportunistički, kontagiozni i zoonotski patogeni, koji izazivaju širok spektar infekcija kod domaćih životinja i ljudi. P. multocida spada u retke uzročnike mastitisa mlečnih krava. Izvor infekcije za mlečnu žlezdu uglavnom ostaje nepoznat, mastitis obično ima akutni tok, a terapija intramamarnom aplikacijom antibiotika ne daje zadovoljavajuće rezultate. Letalni ishod je moguć usled razvoja endotoksemije. U literaturi ima veoma malo podataka o mastitisima mlečnih krava čiji je uzročnik P. multocida, zbog čega u ovom radu opisujemo jedan takav slučaj, uz prikaz osnovnih anamnestičkih podataka, kliničke slike, laboratorijske dijagnostike i terapijskog pristupa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Pasteurella multocida mastitis in cow: Case report, Mastitis kod krave izazvan bakterijom Pasteurella multocida",
volume = "71",
number = "2",
pages = "117-122",
doi = "10.2298/VETGL170619011M"
}

Milanov, D., Aleksić, N., Todorović, D.,& Bugarski, D.. (2017). Pasteurella multocida mastitis in cow: Case report. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 117-122.
https://doi.org/10.2298/VETGL170619011M

Milanov D, Aleksić N, Todorović D, Bugarski D. Pasteurella multocida mastitis in cow: Case report. in Veterinarski Glasnik. 2017;71(2):117-122.
doi:10.2298/VETGL170619011M .

Milanov, Dubravka, Aleksić, Nevenka, Todorović, Dalibor, Bugarski, Dejan, "Pasteurella multocida mastitis in cow: Case report" in Veterinarski Glasnik, 71, no. 2 (2017):117-122,
https://doi.org/10.2298/VETGL170619011M . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Nišavić, Jakov
AU  - Marković, Maja
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1441
AB  - Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL.
AB  - Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibiotic resistance in bacteria isolated from fish in Serbia
T1  - Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije
VL  - 71
IS  - 1
SP  - 24
EP  - 34
DO  - 10.2298/VETGL170329005A
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan",
year = "2017",
abstract = "Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL., Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibiotic resistance in bacteria isolated from fish in Serbia, Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije",
volume = "71",
number = "1",
pages = "24-34",
doi = "10.2298/VETGL170329005A"
}

Aksentijević, K., Ašanin, J., Nišavić, J., Marković, M., Milanov, D.,& Mišić, D.. (2017). Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 24-34.
https://doi.org/10.2298/VETGL170329005A

Aksentijević K, Ašanin J, Nišavić J, Marković M, Milanov D, Mišić D. Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik. 2017;71(1):24-34.
doi:10.2298/VETGL170329005A .

Aksentijević, Ksenija, Ašanin, Jelena, Nišavić, Jakov, Marković, Maja, Milanov, Dubravka, Mišić, Dušan, "Antibiotic resistance in bacteria isolated from fish in Serbia" in Veterinarski Glasnik, 71, no. 1 (2017):24-34,
https://doi.org/10.2298/VETGL170329005A . .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Ilić, Vesna
AU  - Vasić, A.
AU  - Maletić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Savić, S.
AU  - Štukelj, Marina
AU  - Zdravković, Nemanja
AU  - Tzika, E. D.
AU  - Niculae, Mihaela
AU  - Milanov, Dubravka
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1525
AB  - The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms
VL  - 68
IS  - 3
SP  - 347
EP  - 354
DO  - 10.12681/jhvms.15488
ER  - 

@article{
author = "Bojkovski, Jovan and Ilić, Vesna and Vasić, A. and Maletić, Jelena and Vakanjac, Slobodanka and Savić, S. and Štukelj, Marina and Zdravković, Nemanja and Tzika, E. D. and Niculae, Mihaela and Milanov, Dubravka",
year = "2017",
abstract = "The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms",
volume = "68",
number = "3",
pages = "347-354",
doi = "10.12681/jhvms.15488"
}

Bojkovski, J., Ilić, V., Vasić, A., Maletić, J., Vakanjac, S., Savić, S., Štukelj, M., Zdravković, N., Tzika, E. D., Niculae, M.,& Milanov, D.. (2017). Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 68(3), 347-354.
https://doi.org/10.12681/jhvms.15488

Bojkovski J, Ilić V, Vasić A, Maletić J, Vakanjac S, Savić S, Štukelj M, Zdravković N, Tzika ED, Niculae M, Milanov D. Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society. 2017;68(3):347-354.
doi:10.12681/jhvms.15488 .

Bojkovski, Jovan, Ilić, Vesna, Vasić, A., Maletić, Jelena, Vakanjac, Slobodanka, Savić, S., Štukelj, Marina, Zdravković, Nemanja, Tzika, E. D., Niculae, Mihaela, Milanov, Dubravka, "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms" in Journal of the Hellenic Veterinary Medical Society, 68, no. 3 (2017):347-354,
https://doi.org/10.12681/jhvms.15488 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
AU  - Karabasil, Nedjeljko
AU  - Velhner, Maja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1470
AB  - Bacteria belonging to the genus Salmonella are one of the major foodborne pathogens, primarily due to their health and economic impact. The main sources of human infection are poultry products. Salmonella may enter various stages of the poultry production process in many different ways. Biofilm is just one of the recently recognised risk factors, which significantly contributes to the persistence of these bacteria in poultry premises, hatchery cabinets, transportation vehicles, and food and feed factory environment. The control of Salmonella in poultry production is a highly complex and challenging process, additionally complicated by the fact that to date no efficient methods have been developed to eliminate biofilms or prevent their formation. In vitro investigations revealed that the ability to produce biofilm varies between Salmonella serovars, strains of the same serovar and under various environmental conditions. This paper briefly summarises literature data concerning biofilms produced by Salmonella serovars and their influence on the efficacy of control and eradication strategies in the poultry production chain.
PB  - Eugen Ulmer Gmbh Co, Stuttgart
T2  - European Poultry Science
T1  - Biofilm as risk factor for Salmonella contamination in various stages of poultry production
VL  - 81
DO  - 10.1399/eps.2017.190
ER  - 

@article{
author = "Milanov, Dubravka and Ljubojević, Dragana and Čabarkapa, Ivana and Karabasil, Nedjeljko and Velhner, Maja",
year = "2017",
abstract = "Bacteria belonging to the genus Salmonella are one of the major foodborne pathogens, primarily due to their health and economic impact. The main sources of human infection are poultry products. Salmonella may enter various stages of the poultry production process in many different ways. Biofilm is just one of the recently recognised risk factors, which significantly contributes to the persistence of these bacteria in poultry premises, hatchery cabinets, transportation vehicles, and food and feed factory environment. The control of Salmonella in poultry production is a highly complex and challenging process, additionally complicated by the fact that to date no efficient methods have been developed to eliminate biofilms or prevent their formation. In vitro investigations revealed that the ability to produce biofilm varies between Salmonella serovars, strains of the same serovar and under various environmental conditions. This paper briefly summarises literature data concerning biofilms produced by Salmonella serovars and their influence on the efficacy of control and eradication strategies in the poultry production chain.",
publisher = "Eugen Ulmer Gmbh Co, Stuttgart",
journal = "European Poultry Science",
title = "Biofilm as risk factor for Salmonella contamination in various stages of poultry production",
volume = "81",
doi = "10.1399/eps.2017.190"
}

Milanov, D., Ljubojević, D., Čabarkapa, I., Karabasil, N.,& Velhner, M.. (2017). Biofilm as risk factor for Salmonella contamination in various stages of poultry production. in European Poultry Science
Eugen Ulmer Gmbh Co, Stuttgart., 81.
https://doi.org/10.1399/eps.2017.190

Milanov D, Ljubojević D, Čabarkapa I, Karabasil N, Velhner M. Biofilm as risk factor for Salmonella contamination in various stages of poultry production. in European Poultry Science. 2017;81.
doi:10.1399/eps.2017.190 .

Milanov, Dubravka, Ljubojević, Dragana, Čabarkapa, Ivana, Karabasil, Nedjeljko, Velhner, Maja, "Biofilm as risk factor for Salmonella contamination in various stages of poultry production" in European Poultry Science, 81 (2017),
https://doi.org/10.1399/eps.2017.190 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
AU  - Aleksić, Nevenka
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1317
AB  - For decades intensive husbandry has more or less been based on the use of antibiotics in sub-minimum inhibitory concentrations (sub-MIC) aimed at growth promotion. Continuous exposure of animal intestinal microbiota, including opportunistic zoonotic pathogens, to sub-MIC poses a pressure to selection and spread of bacteria strains with developed mechanism of antibiotic resistance. These bacteria may be transferred to people either by direct contact with farm animals or indirectly, via the food chain. Although in the EU a ban on the use of antibiotics as growth promoters was imposed in 2006, in many countries, including the largest producers and consumers of antibiotics in the world, it has yet to be done. Given that we are faced with a global problem of the loss of the efficacy of several antibiotic classes which are available for the treatment of human bacterial infections, it is unacceptable that antibiotic use in husbandry is not under global control. Reduction in antibiotic use in clinical practice in human medicine remains in dispute, but non-therapeutic use in husbandry remains a field in which much can be done to contribute substantially to the extension of antibiotic effectiveness and health care of future generations.
AB  - Intenzivna stočarska proizvodnja se decenijama u većoj ili manjoj meri bazira na upotrebi antibiotika u subinhibitornim koncentracijama za promociju rasta. Kontinuirano izlaganje mikrobiota digestivnog trakta životinja (uključujući i oportunističke zoonotske patogene) sub-inhibitornim koncentracijama antibiotika, predstavlja pritisak na selekciju i širenje sojeva bakterija sa mehanizmima rezistencije na antibiotike. Na ljude se ove bakterije mogu preneti direktnim kontaktom sa farmskim životinjama ili na posredan način, preko lanca ishrane. Iako je u zemljama Evropske unije upotreba antibiotika za promociju rasta životinja zabranjena 2006. godine, u mnogim državama, uključujući najveće proizvođače i potrošače antibiotika u svetu, ova praksa se zadržala do danas. Gubitak efikasnosti antibiotika koji su trenutno na raspolaganju za lečenje bakterijskih infekcija kod ljudi je rastući problem, zbog čega je neprihvatljivo da upotreba antibiotika u stočarskoj proizvodnji nije pod globalnom kontrolom. Klinička praksa u humanoj medicini ostavlja diskutabilan prostor za redukciju primene antibiotika, ali je neterapeutska upotreba u stočarstvu oblast u okviru koje se može značajno doprineti produžavanju veka upotrebljivosti pojedinih klasa antibiotika i očuvanju zdravlja budućih generacija.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Impact of antibiotics used as growth promoters on bacterial resistance
T1  - Uticaj antibiotika koji se koriste kao stimulatori rasta kod životinja na rezistenciju bakterija
VL  - 43
IS  - 2
SP  - 83
EP  - 92
DO  - 10.5937/FFR1602083M
ER  - 

@article{
author = "Milanov, Dubravka and Ljubojević, Dragana and Čabarkapa, Ivana and Aleksić, Nevenka",
year = "2016",
abstract = "For decades intensive husbandry has more or less been based on the use of antibiotics in sub-minimum inhibitory concentrations (sub-MIC) aimed at growth promotion. Continuous exposure of animal intestinal microbiota, including opportunistic zoonotic pathogens, to sub-MIC poses a pressure to selection and spread of bacteria strains with developed mechanism of antibiotic resistance. These bacteria may be transferred to people either by direct contact with farm animals or indirectly, via the food chain. Although in the EU a ban on the use of antibiotics as growth promoters was imposed in 2006, in many countries, including the largest producers and consumers of antibiotics in the world, it has yet to be done. Given that we are faced with a global problem of the loss of the efficacy of several antibiotic classes which are available for the treatment of human bacterial infections, it is unacceptable that antibiotic use in husbandry is not under global control. Reduction in antibiotic use in clinical practice in human medicine remains in dispute, but non-therapeutic use in husbandry remains a field in which much can be done to contribute substantially to the extension of antibiotic effectiveness and health care of future generations., Intenzivna stočarska proizvodnja se decenijama u većoj ili manjoj meri bazira na upotrebi antibiotika u subinhibitornim koncentracijama za promociju rasta. Kontinuirano izlaganje mikrobiota digestivnog trakta životinja (uključujući i oportunističke zoonotske patogene) sub-inhibitornim koncentracijama antibiotika, predstavlja pritisak na selekciju i širenje sojeva bakterija sa mehanizmima rezistencije na antibiotike. Na ljude se ove bakterije mogu preneti direktnim kontaktom sa farmskim životinjama ili na posredan način, preko lanca ishrane. Iako je u zemljama Evropske unije upotreba antibiotika za promociju rasta životinja zabranjena 2006. godine, u mnogim državama, uključujući najveće proizvođače i potrošače antibiotika u svetu, ova praksa se zadržala do danas. Gubitak efikasnosti antibiotika koji su trenutno na raspolaganju za lečenje bakterijskih infekcija kod ljudi je rastući problem, zbog čega je neprihvatljivo da upotreba antibiotika u stočarskoj proizvodnji nije pod globalnom kontrolom. Klinička praksa u humanoj medicini ostavlja diskutabilan prostor za redukciju primene antibiotika, ali je neterapeutska upotreba u stočarstvu oblast u okviru koje se može značajno doprineti produžavanju veka upotrebljivosti pojedinih klasa antibiotika i očuvanju zdravlja budućih generacija.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Impact of antibiotics used as growth promoters on bacterial resistance, Uticaj antibiotika koji se koriste kao stimulatori rasta kod životinja na rezistenciju bakterija",
volume = "43",
number = "2",
pages = "83-92",
doi = "10.5937/FFR1602083M"
}

Milanov, D., Ljubojević, D., Čabarkapa, I.,& Aleksić, N.. (2016). Impact of antibiotics used as growth promoters on bacterial resistance. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 43(2), 83-92.
https://doi.org/10.5937/FFR1602083M

Milanov D, Ljubojević D, Čabarkapa I, Aleksić N. Impact of antibiotics used as growth promoters on bacterial resistance. in Food and Feed Research. 2016;43(2):83-92.
doi:10.5937/FFR1602083M .

Milanov, Dubravka, Ljubojević, Dragana, Čabarkapa, Ivana, Aleksić, Nevenka, "Impact of antibiotics used as growth promoters on bacterial resistance" in Food and Feed Research, 43, no. 2 (2016):83-92,
https://doi.org/10.5937/FFR1602083M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
AU  - Čabarkapa, Ivana
AU  - Ljubojević, Dragana
AU  - Živkov-Baloš, Milica
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1326
AB  - All natural antibiotics available to modern medicine are products of soil-dwelling bacteria and fungi. In addition, all resistance genes which are being detected in human pathogens existed in soil bacteria even before antibiotics were discovered and brought into use. However, the concentrations of natural antibiotics in soil are usually subinhibitory - insufficient for the selection of resistant subpopulations of microorganisms. The consumption of organic fertilisers for agricultural soil amendment increases proportionally to the consumers' growing demand for organically produced food. Manure originating from industrial pig, cattle and poultry farms is not only the source of nutrients which stimulate the vital functions of soil microorganisms, but also of antibiotics and bacteria harbouring various resistance mechanisms. The application of organic fertilizer leads to disruption of the natural balance between bacterial communities in the soil through several mechanisms, and influences the increase in the prevalence of resistance genes and promotes their horizontal transfer. Whether as-yet-unknown resistance genes in soil bacteria may pose threat to human health if transferred from commensal bacteria in the environment to pathogen species, or migrate to clinical settings via food chain or in some other possible route - remains an open question.
AB  - Svi prirodni antibiotici koji su na raspolaganju savremenoj medicini su produkti bakterija i glivica kojima je zemljište prirodno stanište, a svi geni rezistencije koji se danas ustanovljavaju kod humanih patogena postojali su kod bakterija u zemljištu i pre otkrića i upotrebe antibiotika. Međutim, koncentracije prirodnih antibiotika u zemljištu uobičajeno su na nivou subihnibitornih, a time i nedovoljne za selekciju rezistentnih subpopulacija mikroorganizama. Upotreba organskog đubriva za fertilizaciju poljoprivrednog zemljišta, povećava se srazmerno porastu zahteva potrošača za organski proizvednom hranom. Organsko đubrivo poreklom sa industrijalizovanih farmi svinja, goveda i živine, nije samo izvor nutrijenata koji podstiču životne funkcije mikroorganizama u zemljištu, već i antibiotika i bakterija sa raznovrsnim mehanizmima rezistencije. Dodavanje organskog đubriva preko više mehanizama remeti prirodnu ravnotežu zajednice bakterija u zemljištu, utiče na porast prevalencije gena rezistencije i podstiče njihov horizontalni transfer. Otvoreno je pitanje da li do sada neotkriveni geni rezistencije zemljišnih bakterija mogu biti nova pretnja ljudskom zdravlju ukoliko se prenesu sa komensalnih bakterija iz okruženja na patogene vrste, ili lancem ishrane i drugim mogućim putevima, migriraju u kliničke uslove.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer
T1  - Geni rezistencije na antibiotike kod bakterija u zemljištu i uticaj primene organskog đubriva na njihovu prevalenciju i horzontalni transfer
VL  - 9
IS  - 2
SP  - 3
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1326
ER  - 

@article{
author = "Milanov, Dubravka and Mišić, Dušan and Čabarkapa, Ivana and Ljubojević, Dragana and Živkov-Baloš, Milica",
year = "2016",
abstract = "All natural antibiotics available to modern medicine are products of soil-dwelling bacteria and fungi. In addition, all resistance genes which are being detected in human pathogens existed in soil bacteria even before antibiotics were discovered and brought into use. However, the concentrations of natural antibiotics in soil are usually subinhibitory - insufficient for the selection of resistant subpopulations of microorganisms. The consumption of organic fertilisers for agricultural soil amendment increases proportionally to the consumers' growing demand for organically produced food. Manure originating from industrial pig, cattle and poultry farms is not only the source of nutrients which stimulate the vital functions of soil microorganisms, but also of antibiotics and bacteria harbouring various resistance mechanisms. The application of organic fertilizer leads to disruption of the natural balance between bacterial communities in the soil through several mechanisms, and influences the increase in the prevalence of resistance genes and promotes their horizontal transfer. Whether as-yet-unknown resistance genes in soil bacteria may pose threat to human health if transferred from commensal bacteria in the environment to pathogen species, or migrate to clinical settings via food chain or in some other possible route - remains an open question., Svi prirodni antibiotici koji su na raspolaganju savremenoj medicini su produkti bakterija i glivica kojima je zemljište prirodno stanište, a svi geni rezistencije koji se danas ustanovljavaju kod humanih patogena postojali su kod bakterija u zemljištu i pre otkrića i upotrebe antibiotika. Međutim, koncentracije prirodnih antibiotika u zemljištu uobičajeno su na nivou subihnibitornih, a time i nedovoljne za selekciju rezistentnih subpopulacija mikroorganizama. Upotreba organskog đubriva za fertilizaciju poljoprivrednog zemljišta, povećava se srazmerno porastu zahteva potrošača za organski proizvednom hranom. Organsko đubrivo poreklom sa industrijalizovanih farmi svinja, goveda i živine, nije samo izvor nutrijenata koji podstiču životne funkcije mikroorganizama u zemljištu, već i antibiotika i bakterija sa raznovrsnim mehanizmima rezistencije. Dodavanje organskog đubriva preko više mehanizama remeti prirodnu ravnotežu zajednice bakterija u zemljištu, utiče na porast prevalencije gena rezistencije i podstiče njihov horizontalni transfer. Otvoreno je pitanje da li do sada neotkriveni geni rezistencije zemljišnih bakterija mogu biti nova pretnja ljudskom zdravlju ukoliko se prenesu sa komensalnih bakterija iz okruženja na patogene vrste, ili lancem ishrane i drugim mogućim putevima, migriraju u kliničke uslove.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer, Geni rezistencije na antibiotike kod bakterija u zemljištu i uticaj primene organskog đubriva na njihovu prevalenciju i horzontalni transfer",
volume = "9",
number = "2",
pages = "3-16",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1326"
}

Milanov, D., Mišić, D., Čabarkapa, I., Ljubojević, D.,& Živkov-Baloš, M.. (2016). Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(2), 3-16.
https://hdl.handle.net/21.15107/rcub_veterinar_1326

Milanov D, Mišić D, Čabarkapa I, Ljubojević D, Živkov-Baloš M. Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer. in Arhiv veterinarske medicine. 2016;9(2):3-16.
https://hdl.handle.net/21.15107/rcub_veterinar_1326 .

Milanov, Dubravka, Mišić, Dušan, Čabarkapa, Ivana, Ljubojević, Dragana, Živkov-Baloš, Milica, "Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer" in Arhiv veterinarske medicine, 9, no. 2 (2016):3-16,
https://hdl.handle.net/21.15107/rcub_veterinar_1326 .

TY  - JOUR
AU  - Prunić, Bojana
AU  - Milanov, Dubravka
AU  - Velhner, Maja
AU  - Pajić, Marko
AU  - Pavlović, Ljiljana
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1399
AB  - Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period. Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test. Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose. Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories
VL  - 10
IS  - 6
SP  - 662
EP  - 666
DO  - 10.3855/jidc.7313
ER  - 

@article{
author = "Prunić, Bojana and Milanov, Dubravka and Velhner, Maja and Pajić, Marko and Pavlović, Ljiljana and Mišić, Dušan",
year = "2016",
abstract = "Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period. Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test. Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose. Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories",
volume = "10",
number = "6",
pages = "662-666",
doi = "10.3855/jidc.7313"
}

Prunić, B., Milanov, D., Velhner, M., Pajić, M., Pavlović, L.,& Mišić, D.. (2016). Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 10(6), 662-666.
https://doi.org/10.3855/jidc.7313

Prunić B, Milanov D, Velhner M, Pajić M, Pavlović L, Mišić D. Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories. in Journal of Infection in Developing Countries. 2016;10(6):662-666.
doi:10.3855/jidc.7313 .

Prunić, Bojana, Milanov, Dubravka, Velhner, Maja, Pajić, Marko, Pavlović, Ljiljana, Mišić, Dušan, "Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories" in Journal of Infection in Developing Countries, 10, no. 6 (2016):662-666,
https://doi.org/10.3855/jidc.7313 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Polaček, Vladimir
AU  - Suvajdzić, Ljiljana
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1414
AB  - Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Journal of Veterinary Research
T1  - Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms
VL  - 60
IS  - 4
SP  - 373
EP  - 378
DO  - 10.1515/jvetres-2016-0054
ER  - 

@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Polaček, Vladimir and Suvajdzić, Ljiljana and Bojkovski, Jovan",
year = "2016",
abstract = "Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Journal of Veterinary Research",
title = "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms",
volume = "60",
number = "4",
pages = "373-378",
doi = "10.1515/jvetres-2016-0054"
}

Milanov, D., Petrović, T., Polaček, V., Suvajdzić, L.,& Bojkovski, J.. (2016). Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research
Walter De Gruyter Gmbh, Berlin., 60(4), 373-378.
https://doi.org/10.1515/jvetres-2016-0054

Milanov D, Petrović T, Polaček V, Suvajdzić L, Bojkovski J. Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research. 2016;60(4):373-378.
doi:10.1515/jvetres-2016-0054 .

Milanov, Dubravka, Petrović, Tamaš, Polaček, Vladimir, Suvajdzić, Ljiljana, Bojkovski, Jovan, "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms" in Journal of Veterinary Research, 60, no. 4 (2016):373-378,
https://doi.org/10.1515/jvetres-2016-0054 . .

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Vidanović, Dejan
AU  - Suvajdzić, Ljiljana
AU  - Stojanov, Igor
AU  - Krnjaić, Dejan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1191
AB  - Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia
VL  - 65
IS  - 4
SP  - 548
EP  - 556
DO  - 10.1515/acve-2015-0046
ER  - 

@article{
author = "Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Vidanović, Dejan and Suvajdzić, Ljiljana and Stojanov, Igor and Krnjaić, Dejan",
year = "2015",
abstract = "Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia",
volume = "65",
number = "4",
pages = "548-556",
doi = "10.1515/acve-2015-0046"
}

Todorović, D., Velhner, M., Milanov, D., Vidanović, D., Suvajdzić, L., Stojanov, I.,& Krnjaić, D.. (2015). Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 548-556.
https://doi.org/10.1515/acve-2015-0046

Todorović D, Velhner M, Milanov D, Vidanović D, Suvajdzić L, Stojanov I, Krnjaić D. Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):548-556.
doi:10.1515/acve-2015-0046 .

Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Vidanović, Dejan, Suvajdzić, Ljiljana, Stojanov, Igor, Krnjaić, Dejan, "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):548-556,
https://doi.org/10.1515/acve-2015-0046 . .

TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Polaček, Vladimir
AU  - Đurić, Spomenka
AU  - Stojanov, Igor
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1238
AB  - Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. .
AB  - Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region
T1  - Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu
VL  - 8
IS  - 1
SP  - 67
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1238
ER  - 

@article{
author = "Pajić, Marko and Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Polaček, Vladimir and Đurić, Spomenka and Stojanov, Igor",
year = "2015",
abstract = "Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. ., Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region, Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu",
volume = "8",
number = "1",
pages = "67-76",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1238"
}

Pajić, M., Todorović, D., Velhner, M., Milanov, D., Polaček, V., Đurić, S.,& Stojanov, I.. (2015). The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 8(1), 67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238

Pajić M, Todorović D, Velhner M, Milanov D, Polaček V, Đurić S, Stojanov I. The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine. 2015;8(1):67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .

Pajić, Marko, Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Polaček, Vladimir, Đurić, Spomenka, Stojanov, Igor, "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region" in Arhiv veterinarske medicine, 8, no. 1 (2015):67-76,
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .