TY  - JOUR
AU  - Prošić, Isidora
AU  - Milčić Matić, Natalija
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Aksentijević, Ksenija
AU  - Ilić, Milica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Gajdov, Vladimir
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3763
AB  - The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study
VL  - 74
IS  - 1
SP  - 117
EP  - 132
DO  - 10.2478/acve-2024-0009
DO  - 10.2478/acve-2024-0009
ER  - 

@article{
author = "Prošić, Isidora and Milčić Matić, Natalija and Milić, Nenad and Radalj, Andrea and Aksentijević, Ksenija and Ilić, Milica and Nišavić, Jakov and Radojičić, Marina and Gajdov, Vladimir and Krnjaić, Dejan",
year = "2024",
abstract = "The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study",
volume = "74",
number = "1",
pages = "117-132",
doi = "10.2478/acve-2024-0009, 10.2478/acve-2024-0009"
}

Prošić, I., Milčić Matić, N., Milić, N., Radalj, A., Aksentijević, K., Ilić, M., Nišavić, J., Radojičić, M., Gajdov, V.,& Krnjaić, D.. (2024). Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 117-132.
https://doi.org/10.2478/acve-2024-0009

Prošić I, Milčić Matić N, Milić N, Radalj A, Aksentijević K, Ilić M, Nišavić J, Radojičić M, Gajdov V, Krnjaić D. Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria. 2024;74(1):117-132.
doi:10.2478/acve-2024-0009 .

Prošić, Isidora, Milčić Matić, Natalija, Milić, Nenad, Radalj, Andrea, Aksentijević, Ksenija, Ilić, Milica, Nišavić, Jakov, Radojičić, Marina, Gajdov, Vladimir, Krnjaić, Dejan, "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study" in Acta Veterinaria, 74, no. 1 (2024):117-132,
https://doi.org/10.2478/acve-2024-0009 . .

TY  - CONF
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Radalj, Andrea
AU  - Prošić, Isidora
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3097
AB  - Rezistencija bakterija na antibiotike je rastući problem koji je
Svetska zdravstvena organizacija proglasila jednom od najvećih globalnih
pretnji po zdravlje ljudi, pri čemu rezistencija na meticilin kod
Staphylococcus vrsta (MRS) predstavlja jedan od najopasnijih oblika
rezistencije bakterija na antibiotike koja se ispoljava prema svim beta-
laktamima izuzev pete generacije cefalosporina. Meticilin rezistentne
S. aureus (MRSA) su zoonotski agensi koji izazivaju lokalne i sistemske
infekcije i koji usled nedostatka efikasne antibiotske terapije
mogu dovesti do fatalnog ishoda infekcija ljudi i životinja. Monitoring
MRSA je od izuzetnog značaja za razumevanje i predviđanje nastanka
i širenja ovih sojeva i sprovodi se u cilju preduzimanja proaktivnih
mera za sprečavanje daljeg širenja rezistencije. MRSA sojevi u genomu
sadrže mobilne genetičke elemente, tzv. stafilokokni kasetni hromozom
(staphylococcal cassette chromosome – SCC, eng.) sa prisutnim
mecA genom koji kodira sintezu penicillin-vezujućeg proteina
2a (penicillin-binding protein 2a – PBP 2a, eng.). PBP 2a je protein
koji se odlikuje transpeptidazom aktivnošću i predstavlja alternativu
penicillin-vezujućem proteinu 2 (penicillin-binding protein 2 – PBP 2,
eng.) za kojeg se vezuju beta-laktamski antibiotici. Posedovanje pored
PBP 2 i PBP 2a, prema kome beta-laktamskih antibiotika imaju
nizak afinitet vezivanja, čini osnovni mehanizam rezistencije kod MRSA.
Dokazivanje prisustva MRSA vršiće se u kliničkim izolatima poreklom
od obolelih pasa i mačaka i to primenom disk-difuzione metode
kao najčešće korišćene kvalitativne metode za određivanje rezistencije
kod kliničkih izolata bakterija, a zatim i primenom kvantitativne metode
upotrebom E-test traka. Dodatno, MRSA sojevi biće ispitani na
prisustvo PBP 2a brzim kitom koji se zasniva na principu lateks aglutinacije.
Na kraju će MRSA sojevi biti potvrđeni molekularnom detekcijom
gena rezistencije primenom lančane reakcije polimeraze (PCR).
U današnje vreme PCR predstavlja nezaobilaznu proceduru u većini
mikrobioloških laboratorija, a našao je primenu u ispitivanju osetljivosti bakterija na antibiotike dopunjujući klasične metode i pružajući
mogućnost istovremenog ispitivanja prisustva više genetskih determinanti
rezistencije. Metoda PCR u cilju detekcije gena rezistencije će
se vršiti ispitivanjem ekstrahovane DNK S. aureus po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi
vizuelizacije i analize dobijenih PCR produkata.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
T1  - Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)
SP  - 111
EP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3097
ER  - 

@conference{
author = "Krnjaić, Dejan and Radojičić, Marina and Radalj, Andrea and Prošić, Isidora",
year = "2023",
abstract = "Rezistencija bakterija na antibiotike je rastući problem koji je
Svetska zdravstvena organizacija proglasila jednom od najvećih globalnih
pretnji po zdravlje ljudi, pri čemu rezistencija na meticilin kod
Staphylococcus vrsta (MRS) predstavlja jedan od najopasnijih oblika
rezistencije bakterija na antibiotike koja se ispoljava prema svim beta-
laktamima izuzev pete generacije cefalosporina. Meticilin rezistentne
S. aureus (MRSA) su zoonotski agensi koji izazivaju lokalne i sistemske
infekcije i koji usled nedostatka efikasne antibiotske terapije
mogu dovesti do fatalnog ishoda infekcija ljudi i životinja. Monitoring
MRSA je od izuzetnog značaja za razumevanje i predviđanje nastanka
i širenja ovih sojeva i sprovodi se u cilju preduzimanja proaktivnih
mera za sprečavanje daljeg širenja rezistencije. MRSA sojevi u genomu
sadrže mobilne genetičke elemente, tzv. stafilokokni kasetni hromozom
(staphylococcal cassette chromosome – SCC, eng.) sa prisutnim
mecA genom koji kodira sintezu penicillin-vezujućeg proteina
2a (penicillin-binding protein 2a – PBP 2a, eng.). PBP 2a je protein
koji se odlikuje transpeptidazom aktivnošću i predstavlja alternativu
penicillin-vezujućem proteinu 2 (penicillin-binding protein 2 – PBP 2,
eng.) za kojeg se vezuju beta-laktamski antibiotici. Posedovanje pored
PBP 2 i PBP 2a, prema kome beta-laktamskih antibiotika imaju
nizak afinitet vezivanja, čini osnovni mehanizam rezistencije kod MRSA.
Dokazivanje prisustva MRSA vršiće se u kliničkim izolatima poreklom
od obolelih pasa i mačaka i to primenom disk-difuzione metode
kao najčešće korišćene kvalitativne metode za određivanje rezistencije
kod kliničkih izolata bakterija, a zatim i primenom kvantitativne metode
upotrebom E-test traka. Dodatno, MRSA sojevi biće ispitani na
prisustvo PBP 2a brzim kitom koji se zasniva na principu lateks aglutinacije.
Na kraju će MRSA sojevi biti potvrđeni molekularnom detekcijom
gena rezistencije primenom lančane reakcije polimeraze (PCR).
U današnje vreme PCR predstavlja nezaobilaznu proceduru u većini
mikrobioloških laboratorija, a našao je primenu u ispitivanju osetljivosti bakterija na antibiotike dopunjujući klasične metode i pružajući
mogućnost istovremenog ispitivanja prisustva više genetskih determinanti
rezistencije. Metoda PCR u cilju detekcije gena rezistencije će
se vršiti ispitivanjem ekstrahovane DNK S. aureus po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi
vizuelizacije i analize dobijenih PCR produkata.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023",
title = "Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)",
pages = "111-124",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3097"
}

Krnjaić, D., Radojičić, M., Radalj, A.,& Prošić, I.. (2023). Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA). in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 111-124.
https://hdl.handle.net/21.15107/rcub_veterinar_3097

Krnjaić D, Radojičić M, Radalj A, Prošić I. Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA). in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023. 2023;:111-124.
https://hdl.handle.net/21.15107/rcub_veterinar_3097 .

Krnjaić, Dejan, Radojičić, Marina, Radalj, Andrea, Prošić, Isidora, "Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)" in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023 (2023):111-124,
https://hdl.handle.net/21.15107/rcub_veterinar_3097 .

TY  - CONF
AU  - Radojičić, Marina
AU  - Prošić, Isidora
AU  - Ezveđ, Jožef
AU  - Krnjaić, Dejan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3144
AB  - Prisustvo infektivnih bolesti u zoološkim vrtovima ugrožava dobrobit životinja i 
ima dalekosežne posledice po životni vek, reprodukciju i ponašanje životinja. Najčešće 
izolovana vrsta koja izaziva mikozu ptica je Aspergillus fumigatus koji je izolovan u 
95% slučajeva aspergiloze ptica. Nakon prelaska u zatvoren prostor, kod nekoliko pin gvina u zoološkom vrtu pojavili su se respiratorni simptomi. Nakon uzorkovanja bri seva površina i vazduha za mikrobiološki pregled, izvršena je kompletna dezinfekcija 
prostora i opreme, a nakon završne dezinfekcije sprovedena je njena kontrola. Identi fikacija mikroorganizama izvršena je na osnovu morfologije izraslih kolonija, kao i na 
osnovu mikroskopskog pregleda preparata, a broj kolonija određen je prema standar dnim procedurama. Dezinfekcija je izvršena organskim proizvodom koji sadrži organ sku melasu šećerne trske i kulture probiotskih mikroorganizama. Pored dezinfekcije, 
izvršena je i kompletna zamena ventilacionih cevi i filtera za vazduh. Procesi uzorko vanja i identifikacija izraslih kolonija ponovlјeni su nakon dva meseca, odnosno 30 
dana nakon izvršene dezinfekcije, u sklopu kontrole dezinfekcije. Rezultati dobijeni 
pre izvršene dezinfekcije pokazali su povećan broj kolonija A. fumigatus u većini uzo raka. Takođe, primećeno je da je najveći broj kolonija detektovan u podlogama u ko jima su zasejani brisevi uzeti sa ventilacionih otvora kao i iz uzoraka vazduha koji su 
prikuplјeni u blizini ventilacionih cevi. Iz tog razloga, preporučena je detalјna zamena 
kompletnog sistema za ventilaciju. Nakon 30 dana od izvršene dezinfekcije urađena 
je kontorola dezinfekcije gde je u svim uzorcima detektovan očekivan broj kolonija A. 
fumigatus, koji ne predstavlјa pretnju po okolni živi svet, čime je potvrđena efikasnost 
izvršene dezinfekcije i primenjenog dezificijensa. Potrebno je naglasiti da, iako su svi 
uslovi u smeštaju za držanje pingvina adekvatni, ipak može doći do infekcije pingvina 
sa A. fumigatus jer su ptice, a posebno one u zatočeništvu, predisponirane ka infekciji 
ovim uzročnikom o čemu svedoči više slučajeva izbijanja zaraze sa A. fumigatus kod 
pingvina u zoološkim vrtovima.
AB  - The spread of infectious diseases in zoo gardens endangers animals’ welfare and 
has significant consequences on their lifespan, reproduction and behavior. Bird mycosis is frequently associated with Aspergillus fumigatus, which is the etiologic agent 
in 95% of all aspergilosis cases in birds. Respiratory signs had been observed in penguins in the zoo, after the transfer to their enclosure. Air and surface swabs were taken for microbiological examination, after which complete desinfection of the enclosure and equipment was performed. Following desinfection, control swabs were taken in order to check the desinfection efficasy. Identification of the microorganisms was based on colony morpholgy and examination of microscopic slide preparations, and colony number was determined by using standard procedures. The desinfectant is an organic compound which contains organic sugar cane molasses and probiotic microorganism cultures. Beside disinfection, complete replacement of ventilation pipes and air filters was performed. Each procedure was repeated after two months, ie 30 days after disinfection, as part of disinfection control. The results showed an increased number of A. fumigatus colonies in most samples. Moreover, it was noticed that the largest number of colonies was detected in swab samples from ventilation and air samples collected near ventilation pipes. For this reason, replacement of the complete ventilation system was recommended. Thirty days after disinfection, another control was performed, where an expected number of A. fumigatus colonies was detected in all samples, which does not pose a threat for animals or visitors, thus the effectiveness of the disinfection and the disinfectant was confirmed. It should be emphasized that, 
although all the conditions in the penguin enclosure are adequate, an infection with A. 
fumigatus can occur in penguins because birds, especially those in captivity, are predisposed to infection with this pathogen, as evidenced by several outbreaks of A. fumigatus in penguins in zoo gardens.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022
T1  - Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja
SP  - 119
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3144
ER  - 

@conference{
author = "Radojičić, Marina and Prošić, Isidora and Ezveđ, Jožef and Krnjaić, Dejan",
year = "2022",
abstract = "Prisustvo infektivnih bolesti u zoološkim vrtovima ugrožava dobrobit životinja i 
ima dalekosežne posledice po životni vek, reprodukciju i ponašanje životinja. Najčešće 
izolovana vrsta koja izaziva mikozu ptica je Aspergillus fumigatus koji je izolovan u 
95% slučajeva aspergiloze ptica. Nakon prelaska u zatvoren prostor, kod nekoliko pin gvina u zoološkom vrtu pojavili su se respiratorni simptomi. Nakon uzorkovanja bri seva površina i vazduha za mikrobiološki pregled, izvršena je kompletna dezinfekcija 
prostora i opreme, a nakon završne dezinfekcije sprovedena je njena kontrola. Identi fikacija mikroorganizama izvršena je na osnovu morfologije izraslih kolonija, kao i na 
osnovu mikroskopskog pregleda preparata, a broj kolonija određen je prema standar dnim procedurama. Dezinfekcija je izvršena organskim proizvodom koji sadrži organ sku melasu šećerne trske i kulture probiotskih mikroorganizama. Pored dezinfekcije, 
izvršena je i kompletna zamena ventilacionih cevi i filtera za vazduh. Procesi uzorko vanja i identifikacija izraslih kolonija ponovlјeni su nakon dva meseca, odnosno 30 
dana nakon izvršene dezinfekcije, u sklopu kontrole dezinfekcije. Rezultati dobijeni 
pre izvršene dezinfekcije pokazali su povećan broj kolonija A. fumigatus u većini uzo raka. Takođe, primećeno je da je najveći broj kolonija detektovan u podlogama u ko jima su zasejani brisevi uzeti sa ventilacionih otvora kao i iz uzoraka vazduha koji su 
prikuplјeni u blizini ventilacionih cevi. Iz tog razloga, preporučena je detalјna zamena 
kompletnog sistema za ventilaciju. Nakon 30 dana od izvršene dezinfekcije urađena 
je kontorola dezinfekcije gde je u svim uzorcima detektovan očekivan broj kolonija A. 
fumigatus, koji ne predstavlјa pretnju po okolni živi svet, čime je potvrđena efikasnost 
izvršene dezinfekcije i primenjenog dezificijensa. Potrebno je naglasiti da, iako su svi 
uslovi u smeštaju za držanje pingvina adekvatni, ipak može doći do infekcije pingvina 
sa A. fumigatus jer su ptice, a posebno one u zatočeništvu, predisponirane ka infekciji 
ovim uzročnikom o čemu svedoči više slučajeva izbijanja zaraze sa A. fumigatus kod 
pingvina u zoološkim vrtovima., The spread of infectious diseases in zoo gardens endangers animals’ welfare and 
has significant consequences on their lifespan, reproduction and behavior. Bird mycosis is frequently associated with Aspergillus fumigatus, which is the etiologic agent 
in 95% of all aspergilosis cases in birds. Respiratory signs had been observed in penguins in the zoo, after the transfer to their enclosure. Air and surface swabs were taken for microbiological examination, after which complete desinfection of the enclosure and equipment was performed. Following desinfection, control swabs were taken in order to check the desinfection efficasy. Identification of the microorganisms was based on colony morpholgy and examination of microscopic slide preparations, and colony number was determined by using standard procedures. The desinfectant is an organic compound which contains organic sugar cane molasses and probiotic microorganism cultures. Beside disinfection, complete replacement of ventilation pipes and air filters was performed. Each procedure was repeated after two months, ie 30 days after disinfection, as part of disinfection control. The results showed an increased number of A. fumigatus colonies in most samples. Moreover, it was noticed that the largest number of colonies was detected in swab samples from ventilation and air samples collected near ventilation pipes. For this reason, replacement of the complete ventilation system was recommended. Thirty days after disinfection, another control was performed, where an expected number of A. fumigatus colonies was detected in all samples, which does not pose a threat for animals or visitors, thus the effectiveness of the disinfection and the disinfectant was confirmed. It should be emphasized that, 
although all the conditions in the penguin enclosure are adequate, an infection with A. 
fumigatus can occur in penguins because birds, especially those in captivity, are predisposed to infection with this pathogen, as evidenced by several outbreaks of A. fumigatus in penguins in zoo gardens.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022",
title = "Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja",
pages = "119-125",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3144"
}

Radojičić, M., Prošić, I., Ezveđ, J.,& Krnjaić, D.. (2022). Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja. in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022
Beograd : Srpsko veterinarsko društvo., 119-125.
https://hdl.handle.net/21.15107/rcub_veterinar_3144

Radojičić M, Prošić I, Ezveđ J, Krnjaić D. Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja. in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022. 2022;:119-125.
https://hdl.handle.net/21.15107/rcub_veterinar_3144 .

Radojičić, Marina, Prošić, Isidora, Ezveđ, Jožef, Krnjaić, Dejan, "Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja" in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022 (2022):119-125,
https://hdl.handle.net/21.15107/rcub_veterinar_3144 .

TY  - JOUR
AU  - Sapundžić Zurovac, Zorana
AU  - Žutić, Jadranka
AU  - Stević, Nataša
AU  - Milićević, Vesna
AU  - Radojičić, Marina
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2510
AB  - Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.
PB  - MDPI
T2  - Veterinary Sciences
T1  - First Report of Brucella Seroprevalence in Wild Boar Population in Serbia
VL  - 9
IS  - 10
SP  - 575
DO  - 10.3390/vetsci9100575
ER  - 

@article{
author = "Sapundžić Zurovac, Zorana and Žutić, Jadranka and Stević, Nataša and Milićević, Vesna and Radojičić, Marina and Stanojević, Slavoljub and Radojičić, Sonja",
year = "2022",
abstract = "Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.",
publisher = "MDPI",
journal = "Veterinary Sciences",
title = "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia",
volume = "9",
number = "10",
pages = "575",
doi = "10.3390/vetsci9100575"
}

Sapundžić Zurovac, Z., Žutić, J., Stević, N., Milićević, V., Radojičić, M., Stanojević, S.,& Radojičić, S.. (2022). First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences
MDPI., 9(10), 575.
https://doi.org/10.3390/vetsci9100575

Sapundžić Zurovac Z, Žutić J, Stević N, Milićević V, Radojičić M, Stanojević S, Radojičić S. First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences. 2022;9(10):575.
doi:10.3390/vetsci9100575 .

Sapundžić Zurovac, Zorana, Žutić, Jadranka, Stević, Nataša, Milićević, Vesna, Radojičić, Marina, Stanojević, Slavoljub, Radojičić, Sonja, "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia" in Veterinary Sciences, 9, no. 10 (2022):575,
https://doi.org/10.3390/vetsci9100575 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Krnjaić, Dejan
AU  - Milićević, Dragan
AU  - Knežević, Aleksandra
AU  - Radojičić, Marina
AU  - Obrenović, Sonja
AU  - Ćosić, Milivoje
AU  - Tešović, Bojana
AU  - Benković, Damir
AU  - Živulj Aleksandar
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2044
AB  - Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.
AB  - Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia
T1  - Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji
VL  - 71
IS  - 1
SP  - 32
EP  - 46
DO  - 10.2478/acve-2021-0003
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Krnjaić, Dejan and Milićević, Dragan and Knežević, Aleksandra and Radojičić, Marina and Obrenović, Sonja and Ćosić, Milivoje and Tešović, Bojana and Benković, Damir and Živulj Aleksandar",
year = "2021",
abstract = "Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars., Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia, Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji",
volume = "71",
number = "1",
pages = "32-46",
doi = "10.2478/acve-2021-0003"
}

Nišavić, J., Milić, N., Radalj, A., Krnjaić, D., Milićević, D., Knežević, A., Radojičić, M., Obrenović, S., Ćosić, M., Tešović, B., Benković, D.,& Živulj Aleksandar. (2021). Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 32-46.
https://doi.org/10.2478/acve-2021-0003

Nišavić J, Milić N, Radalj A, Krnjaić D, Milićević D, Knežević A, Radojičić M, Obrenović S, Ćosić M, Tešović B, Benković D, Živulj Aleksandar. Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd. 2021;71(1):32-46.
doi:10.2478/acve-2021-0003 .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Krnjaić, Dejan, Milićević, Dragan, Knežević, Aleksandra, Radojičić, Marina, Obrenović, Sonja, Ćosić, Milivoje, Tešović, Bojana, Benković, Damir, Živulj Aleksandar, "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia" in Acta Veterinaria-Beograd, 71, no. 1 (2021):32-46,
https://doi.org/10.2478/acve-2021-0003 . .

TY  - CONF
AU  - Radojičić, Marina
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Nišavić, Jakov
AU  - Radalj, Andrea
AU  - Prošić, Isidora
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2263
AB  - Today, pets are the source of numerous infectious diseases that can transmissible
to humans resulting from their increasingly frequent contact. The most notable
viruses with zoonotic potential include rabies and influenza viruses as well as
rotaviruses and noroviruses. However, the importance of individual viruses varies
depending on the climate and infectious disease control systems in certain
countries. Dogs, cats, and other increasingly popular pet species can transmit
bacterial zoonotic agents to humans in various ways. Aside from well-known
pathogens such as the bacteria causing leptospirosis, salmonellosis,
campylobacteriosis, or brucellosis, the bacteria Pasteurella multocida and
Bartonella henselae transmitted by bites or scratches are also significant in
human pathology. There has been a notable increase in the prevalence of
methicillin-resistant strains of Staphylococcus aureus (MRSA) in isolates
originating from pets and the transmission of these strains between humans and
animals requires special attention. Furthermore, fungi causing diseases such as
sporotrichosis or dermatophytosis are linked to long-term and persistent
infections in humans. The epidemiological situation caused by SARS-CoV-2, and
the assumption of an interspecies jump of this virus from animals to humans,
including its documented presence in domestic cats, dogs, tigers, and martens,
have raised the question of the possibility of virus transmission from pets to
humans. However, the current pandemic is caused solely by SARS-CoV-2
transmission in the human population, and these animals are not a source of infection for humans. A significant number of zoonoses originating from pets
pose a threat to public health, thus requiring the "One Health" approach through
close cooperation between human and veterinary medicine to develop and
implement effective health measures for both humans and animals. Veterinarians
must inform pet owners about infectious disease prevention measures and risks
posed by keeping certain species of animals as a part of responsible ownership.
AB  - Кућни љубимци у данашње време представљају извор великог броја
инфективних обољења преносивих на људе као последица њиховог све
учесталијег контакта. У најважније вирусе са зоонотским потенцијалом
спадају вируси беснила и инфлуенце као и ротавируси и норовируси,
међутим, значај појединих вируса варира зависно од поднебља као и система
сузбијања заразних болести у појединим државама. Пси и мачке, као и све
популарније остале врсте љубимаца, на различите начине могу пренети
узрочнике бактеријских зооноза на човека. Тако на пример, бактерије које се
преносе уједом или путем огреботина као што су Pasteurella multocida или
Bartonella henselae, поред познатих узрочника обољења попут лептоспирозе,
салмонелозе, кампилобактериозе или бруцелозе, немају занемарљив значај у
патологији људи. Последњих година је доказан пораст преваленције
метицилин резистентних сојева Staphylococcus aureus (MRSA) у изолатима
пореклом од кућних љубимаца, а великa пажња се придаје управо трансмисији
ових сојева између људи и животиња. Поред бактерија и вируса, гљивице, као
што су узрочници споротрихозе или дерматофитозе, често представљају узрок
дуготрајних и упорних инфекција људи. Епидемиолошка ситуација у свету
изазвана SARS-CoV-2 и претпоставка о интерспецијском скоку овог вируса са
животиња на човека, као и његово присуство детектовно код домаћих мачака,
паса, тигрова и куна, довеле су у питање могућност преношења вируса са
кућних љубимаца на људе. Неопходно је напоменути да је тренутна пандемија изазвана искључиво трансмисијом SARS-CoV-2 у хуманој популацији и да
наведене животиње нису извор инфекције за човека. Значајан број зооноза
пореклом од кућних љубимаца представља претњу по јавно здравље при чему
је неопходна примена концепта јединственог здравља која подразумева
блиску сарадњу хумане и ветеринарске медицине са циљем развоја и примене
ефикасних мера здравствене заштите људи и животиња. У склопу одговорног
власништва, власници кућних љубимаца од стране ветеринара морају бити
обавештени о мерама превенције инфективних болести и појединим
ризицима које носи држање одређених врста животиња.
C3  - 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske
T1  - Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca
T1  - Bacterial, fungal, and viral zoonoses of pets
SP  - 49
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2263
ER  - 

@conference{
author = "Radojičić, Marina and Milić, Nenad and Krnjaić, Dejan and Nišavić, Jakov and Radalj, Andrea and Prošić, Isidora",
year = "2021",
abstract = "Today, pets are the source of numerous infectious diseases that can transmissible
to humans resulting from their increasingly frequent contact. The most notable
viruses with zoonotic potential include rabies and influenza viruses as well as
rotaviruses and noroviruses. However, the importance of individual viruses varies
depending on the climate and infectious disease control systems in certain
countries. Dogs, cats, and other increasingly popular pet species can transmit
bacterial zoonotic agents to humans in various ways. Aside from well-known
pathogens such as the bacteria causing leptospirosis, salmonellosis,
campylobacteriosis, or brucellosis, the bacteria Pasteurella multocida and
Bartonella henselae transmitted by bites or scratches are also significant in
human pathology. There has been a notable increase in the prevalence of
methicillin-resistant strains of Staphylococcus aureus (MRSA) in isolates
originating from pets and the transmission of these strains between humans and
animals requires special attention. Furthermore, fungi causing diseases such as
sporotrichosis or dermatophytosis are linked to long-term and persistent
infections in humans. The epidemiological situation caused by SARS-CoV-2, and
the assumption of an interspecies jump of this virus from animals to humans,
including its documented presence in domestic cats, dogs, tigers, and martens,
have raised the question of the possibility of virus transmission from pets to
humans. However, the current pandemic is caused solely by SARS-CoV-2
transmission in the human population, and these animals are not a source of infection for humans. A significant number of zoonoses originating from pets
pose a threat to public health, thus requiring the "One Health" approach through
close cooperation between human and veterinary medicine to develop and
implement effective health measures for both humans and animals. Veterinarians
must inform pet owners about infectious disease prevention measures and risks
posed by keeping certain species of animals as a part of responsible ownership., Кућни љубимци у данашње време представљају извор великог броја
инфективних обољења преносивих на људе као последица њиховог све
учесталијег контакта. У најважније вирусе са зоонотским потенцијалом
спадају вируси беснила и инфлуенце као и ротавируси и норовируси,
међутим, значај појединих вируса варира зависно од поднебља као и система
сузбијања заразних болести у појединим државама. Пси и мачке, као и све
популарније остале врсте љубимаца, на различите начине могу пренети
узрочнике бактеријских зооноза на човека. Тако на пример, бактерије које се
преносе уједом или путем огреботина као што су Pasteurella multocida или
Bartonella henselae, поред познатих узрочника обољења попут лептоспирозе,
салмонелозе, кампилобактериозе или бруцелозе, немају занемарљив значај у
патологији људи. Последњих година је доказан пораст преваленције
метицилин резистентних сојева Staphylococcus aureus (MRSA) у изолатима
пореклом од кућних љубимаца, а великa пажња се придаје управо трансмисији
ових сојева између људи и животиња. Поред бактерија и вируса, гљивице, као
што су узрочници споротрихозе или дерматофитозе, често представљају узрок
дуготрајних и упорних инфекција људи. Епидемиолошка ситуација у свету
изазвана SARS-CoV-2 и претпоставка о интерспецијском скоку овог вируса са
животиња на човека, као и његово присуство детектовно код домаћих мачака,
паса, тигрова и куна, довеле су у питање могућност преношења вируса са
кућних љубимаца на људе. Неопходно је напоменути да је тренутна пандемија изазвана искључиво трансмисијом SARS-CoV-2 у хуманој популацији и да
наведене животиње нису извор инфекције за човека. Значајан број зооноза
пореклом од кућних љубимаца представља претњу по јавно здравље при чему
је неопходна примена концепта јединственог здравља која подразумева
блиску сарадњу хумане и ветеринарске медицине са циљем развоја и примене
ефикасних мера здравствене заштите људи и животиња. У склопу одговорног
власништва, власници кућних љубимаца од стране ветеринара морају бити
обавештени о мерама превенције инфективних болести и појединим
ризицима које носи држање одређених врста животиња.",
journal = "26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske",
title = "Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca, Bacterial, fungal, and viral zoonoses of pets",
pages = "49-52",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2263"
}

Radojičić, M., Milić, N., Krnjaić, D., Nišavić, J., Radalj, A.,& Prošić, I.. (2021). Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca. in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske, 49-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2263

Radojičić M, Milić N, Krnjaić D, Nišavić J, Radalj A, Prošić I. Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca. in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske. 2021;:49-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2263 .

Radojičić, Marina, Milić, Nenad, Krnjaić, Dejan, Nišavić, Jakov, Radalj, Andrea, Prošić, Isidora, "Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca" in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske (2021):49-52,
https://hdl.handle.net/21.15107/rcub_veterinar_2263 .

TY  - CONF
AU  - Čobanović, Nikola
AU  - Radojičić, Marina
AU  - Suvajdžić, Branko
AU  - Vasilev, Dragan
AU  - Karabasil, Nedjeljko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2282
AB  - This study aimed to examine the effects of handling procedure during unloading on blood glucose level, carcass lesions and meat quality of market-weight pigs. Rough handling during unloading was related to higher blood glucose level and frequency of slipping and falling. In contrast, gentle handling during unloading was related to the lower blood glucose level and frequency of slipping and falling, but the higher frequency of reluctance to move and turning back. Rough handling during unloading resulted in a higher carcass lesion score, and the higher tendency towards lesions on the middle part of the carcass and handling-type carcass lesions. Pigs subjected to rough handling during unloading had a higher meat temperature 45 minutes after slaughter, lower meat pH value 45 minutes and 24 hours postmortem, higher drip and cooking loss, higher L∗ and b∗ values and lower sensory colour score, and consequently, produced a higher prevalence of pale, soft and exudative meat. In contrast, pigs exposed to gentle handling during unloading produced a lower percentage of pale, soft and exudative meat, but a higher percentage of pale, firm, and nonexudative. In conclusion, gentle handling during unloading resulted in improved animal welfare, decreased stress intensity, and increased pork quality.
PB  - IOP Publishing Ltd
C3  - 61st International Meat Industry Conference (MEATCON2021)
T1  - Effects of handling procedure during unloading on welfare and meat quality of market-weight pigs
VL  - 854
SP  - 012017
DO  - 10.1088/1755-1315/854/1/012017
ER  - 

@conference{
author = "Čobanović, Nikola and Radojičić, Marina and Suvajdžić, Branko and Vasilev, Dragan and Karabasil, Nedjeljko",
year = "2021",
abstract = "This study aimed to examine the effects of handling procedure during unloading on blood glucose level, carcass lesions and meat quality of market-weight pigs. Rough handling during unloading was related to higher blood glucose level and frequency of slipping and falling. In contrast, gentle handling during unloading was related to the lower blood glucose level and frequency of slipping and falling, but the higher frequency of reluctance to move and turning back. Rough handling during unloading resulted in a higher carcass lesion score, and the higher tendency towards lesions on the middle part of the carcass and handling-type carcass lesions. Pigs subjected to rough handling during unloading had a higher meat temperature 45 minutes after slaughter, lower meat pH value 45 minutes and 24 hours postmortem, higher drip and cooking loss, higher L∗ and b∗ values and lower sensory colour score, and consequently, produced a higher prevalence of pale, soft and exudative meat. In contrast, pigs exposed to gentle handling during unloading produced a lower percentage of pale, soft and exudative meat, but a higher percentage of pale, firm, and nonexudative. In conclusion, gentle handling during unloading resulted in improved animal welfare, decreased stress intensity, and increased pork quality.",
publisher = "IOP Publishing Ltd",
journal = "61st International Meat Industry Conference (MEATCON2021)",
title = "Effects of handling procedure during unloading on welfare and meat quality of market-weight pigs",
volume = "854",
pages = "012017",
doi = "10.1088/1755-1315/854/1/012017"
}

Čobanović, N., Radojičić, M., Suvajdžić, B., Vasilev, D.,& Karabasil, N.. (2021). Effects of handling procedure during unloading on welfare and meat quality of market-weight pigs. in 61st International Meat Industry Conference (MEATCON2021)
IOP Publishing Ltd., 854, 012017.
https://doi.org/10.1088/1755-1315/854/1/012017

Čobanović N, Radojičić M, Suvajdžić B, Vasilev D, Karabasil N. Effects of handling procedure during unloading on welfare and meat quality of market-weight pigs. in 61st International Meat Industry Conference (MEATCON2021). 2021;854:012017.
doi:10.1088/1755-1315/854/1/012017 .

Čobanović, Nikola, Radojičić, Marina, Suvajdžić, Branko, Vasilev, Dragan, Karabasil, Nedjeljko, "Effects of handling procedure during unloading on welfare and meat quality of market-weight pigs" in 61st International Meat Industry Conference (MEATCON2021), 854 (2021):012017,
https://doi.org/10.1088/1755-1315/854/1/012017 . .

TY  - CONF
AU  - Radojičić, Marina
AU  - Marković, Maja
AU  - Milić, Nenad
AU  - Kulišić, Zoran
AU  - Radalj, Andrea
AU  - Krnjaić, Dejan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2265
AB  - Mnogi zdravstveni problemi ljudi predstavljaju direktnu posledicu intezivnog kontakta sa
životinjama, pri čemu najmanje 75% novih infektivnih i pretećih infektivnih bolesti pripada
zoonozama ili vektorski prenosivim bolestima. Kućni ljubimci na različite načine ljudima mogu
preneti veliki broj infektivnih oboljenja. U najznačajnije zoonoze se svrstavaju infekcije izazvane
virusom besnila, norovirusima, rotavirusima ili virusima influence, kao i veliki broj bakterijskih
infekcija kao što su salmoneloza, kampilobakterioza, bruceloza i leptospiroza. Bakterijske
zoonoze ukjlučuju i bakterije koje se prenose ujedom ili putem ogrebotina kao što su Pasteurella
multocida ili Bartonella henselae. Sve veći značaj pridaje se transmisiji meticilin rezistentnih
sojeva Staphylococcus aureus (MRSA) između ljudi i životinja, a dokazan je i porast prevalencije
ovih bakterija u izolatima poreklom iz kućnih ljubimaca. Ne treba zanemariti ni činjenicu da psi i
mačke takođe mogu biti izvor gljivičnih infekcija kao što su sporotrihoza ili dermatofitoza. U
najznačajnije zoonotske patogene parazitske etiologije spadaju uzročnici toksoplazmoze,
đardioze, toksokarioze, onhocerkoze i ehinokokoze. Pored toga, sa globalnim otopljavanjem sve
su rasprostranjenije i vektorski prenosive zoonoze izazvane sa: Anaplasma phagocytophilum,
Rickettsia felis, Borrelia burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis
i Dirofilaria repens koje se putem krpelja, buva, peščanih mušica i komaraca prenose sa kućnih
ljubimaca na ljude. S obzirom da veliki broj pomenutih zoonoza predstavlja pretnju po javno
zdravlje, neophodno je pristupiti ovom problemu sa dve strane. Veterinari moraju upoznati
vlasnike sa metodama prevencije infektivnih bolesti kućnih ljubimaca, a koje podrazumevaju
odgovorno vlasništvo. S druge strane, lekari moraju imati u vidu mogućnost pojave zoonoza i
savetovati pacijente u cilju sprečavanja infekcije ljudi. Primenom koncepta jedinstvenog zdravlja
neophodno je uspostavljanje bliže saradnje lekara i veterinara, a jačanjem epidemioloških i
laboratorijskih ispitivanja moguće je utvrđivanje prisustva, raširenosti i rizika od pojave zoonoza
ljudi poreklom od kućnih ljubimaca, a zajedničkim radom mogu se razviti i primeniti održive i
efektivne mere zaštite zdravlja ljudi i životinja.
AB  - Many emerging health issues are linked to increasing contact between humans and animals, and
it is estimated that at least 75% of emerging and re-emerging diseases are either zoonotic or
vector-borne. Pets may transmit a variety of infectious diseases to humans. Viral diseases caused
by rabies virus, noroviruses, rotaviruses or influenza viruses, as well as numerous bacterial
infections such as salmonellosis, campylobacteriosis, brucellosis and leptospirosis present some
of the most significant zoonoses linked to household pets. Bacterial zoonoses also include bacteria
transmitted by bites or scratches like Pasteurella multocida or Bartonella henselae. More attention
is paid to the transmission of methicilin resistant strains of Staphylococcus aureus (MRSA)
between humans and animals, since the prevalence of these isolates from samples of animal origin
is on the rise. Moreover, the fact that dogs and cats can be sources of serious fungal infections such
as sporotrichosis or dermatophytosis shouldn’t be overseen. Most important zoonotic parasites
are the causative agents of toxoplasmosis, giardiasis, toxocariasis, onchocercosis and
echinococcosis. One of the apparent consequences of global warming is the increased prevalence
of vector borne zoonotic diseases caused by: Anaplasma phagocytophilum, Rickettsia felis, Borrelia
burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis and Dirofilaria repens
transmitted from animals to humans by ticks, fleas, phlebotomine sand flies and mosquitoes.
Considering that many of the mentioned zoonoses pose a serious risk to public health, it is
essential to address this problem from two sides. Veterinarians must instruct the owners on
necessary preventive measures implying responsible pet ownership. On the other hand,
physicians must maintain awareness of pet infectious diseases and counsel patients to prevent
human infection. By strengthening epidemiologic and laboratory investigations that assess the
role of environmental influences, this partnership can help develop and apply sustainable and
effective community health interventions.
PB  - Udruženje mikrobiologa Srbije, Beograd
C3  - XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
T1  - Kućni ljubimci i zoonoze
T1  - Pets and zoonoses
SP  - 153
EP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2265
ER  - 

@conference{
author = "Radojičić, Marina and Marković, Maja and Milić, Nenad and Kulišić, Zoran and Radalj, Andrea and Krnjaić, Dejan",
year = "2018",
abstract = "Mnogi zdravstveni problemi ljudi predstavljaju direktnu posledicu intezivnog kontakta sa
životinjama, pri čemu najmanje 75% novih infektivnih i pretećih infektivnih bolesti pripada
zoonozama ili vektorski prenosivim bolestima. Kućni ljubimci na različite načine ljudima mogu
preneti veliki broj infektivnih oboljenja. U najznačajnije zoonoze se svrstavaju infekcije izazvane
virusom besnila, norovirusima, rotavirusima ili virusima influence, kao i veliki broj bakterijskih
infekcija kao što su salmoneloza, kampilobakterioza, bruceloza i leptospiroza. Bakterijske
zoonoze ukjlučuju i bakterije koje se prenose ujedom ili putem ogrebotina kao što su Pasteurella
multocida ili Bartonella henselae. Sve veći značaj pridaje se transmisiji meticilin rezistentnih
sojeva Staphylococcus aureus (MRSA) između ljudi i životinja, a dokazan je i porast prevalencije
ovih bakterija u izolatima poreklom iz kućnih ljubimaca. Ne treba zanemariti ni činjenicu da psi i
mačke takođe mogu biti izvor gljivičnih infekcija kao što su sporotrihoza ili dermatofitoza. U
najznačajnije zoonotske patogene parazitske etiologije spadaju uzročnici toksoplazmoze,
đardioze, toksokarioze, onhocerkoze i ehinokokoze. Pored toga, sa globalnim otopljavanjem sve
su rasprostranjenije i vektorski prenosive zoonoze izazvane sa: Anaplasma phagocytophilum,
Rickettsia felis, Borrelia burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis
i Dirofilaria repens koje se putem krpelja, buva, peščanih mušica i komaraca prenose sa kućnih
ljubimaca na ljude. S obzirom da veliki broj pomenutih zoonoza predstavlja pretnju po javno
zdravlje, neophodno je pristupiti ovom problemu sa dve strane. Veterinari moraju upoznati
vlasnike sa metodama prevencije infektivnih bolesti kućnih ljubimaca, a koje podrazumevaju
odgovorno vlasništvo. S druge strane, lekari moraju imati u vidu mogućnost pojave zoonoza i
savetovati pacijente u cilju sprečavanja infekcije ljudi. Primenom koncepta jedinstvenog zdravlja
neophodno je uspostavljanje bliže saradnje lekara i veterinara, a jačanjem epidemioloških i
laboratorijskih ispitivanja moguće je utvrđivanje prisustva, raširenosti i rizika od pojave zoonoza
ljudi poreklom od kućnih ljubimaca, a zajedničkim radom mogu se razviti i primeniti održive i
efektivne mere zaštite zdravlja ljudi i životinja., Many emerging health issues are linked to increasing contact between humans and animals, and
it is estimated that at least 75% of emerging and re-emerging diseases are either zoonotic or
vector-borne. Pets may transmit a variety of infectious diseases to humans. Viral diseases caused
by rabies virus, noroviruses, rotaviruses or influenza viruses, as well as numerous bacterial
infections such as salmonellosis, campylobacteriosis, brucellosis and leptospirosis present some
of the most significant zoonoses linked to household pets. Bacterial zoonoses also include bacteria
transmitted by bites or scratches like Pasteurella multocida or Bartonella henselae. More attention
is paid to the transmission of methicilin resistant strains of Staphylococcus aureus (MRSA)
between humans and animals, since the prevalence of these isolates from samples of animal origin
is on the rise. Moreover, the fact that dogs and cats can be sources of serious fungal infections such
as sporotrichosis or dermatophytosis shouldn’t be overseen. Most important zoonotic parasites
are the causative agents of toxoplasmosis, giardiasis, toxocariasis, onchocercosis and
echinococcosis. One of the apparent consequences of global warming is the increased prevalence
of vector borne zoonotic diseases caused by: Anaplasma phagocytophilum, Rickettsia felis, Borrelia
burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis and Dirofilaria repens
transmitted from animals to humans by ticks, fleas, phlebotomine sand flies and mosquitoes.
Considering that many of the mentioned zoonoses pose a serious risk to public health, it is
essential to address this problem from two sides. Veterinarians must instruct the owners on
necessary preventive measures implying responsible pet ownership. On the other hand,
physicians must maintain awareness of pet infectious diseases and counsel patients to prevent
human infection. By strengthening epidemiologic and laboratory investigations that assess the
role of environmental influences, this partnership can help develop and apply sustainable and
effective community health interventions.",
publisher = "Udruženje mikrobiologa Srbije, Beograd",
journal = "XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO",
title = "Kućni ljubimci i zoonoze, Pets and zoonoses",
pages = "153-154",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2265"
}

Radojičić, M., Marković, M., Milić, N., Kulišić, Z., Radalj, A.,& Krnjaić, D.. (2018). Kućni ljubimci i zoonoze. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
Udruženje mikrobiologa Srbije, Beograd., 153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2265

Radojičić M, Marković M, Milić N, Kulišić Z, Radalj A, Krnjaić D. Kućni ljubimci i zoonoze. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO. 2018;:153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2265 .

Radojičić, Marina, Marković, Maja, Milić, Nenad, Kulišić, Zoran, Radalj, Andrea, Krnjaić, Dejan, "Kućni ljubimci i zoonoze" in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO (2018):153-154,
https://hdl.handle.net/21.15107/rcub_veterinar_2265 .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1453
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .

TY  - JOUR
AU  - Bošnjak, Ivan
AU  - Zdravković, Nemanja
AU  - Čolović, Svetlana
AU  - Ranđelović, Slađana
AU  - Galić, Nataša
AU  - Radojičić, Marina
AU  - Šekler, Milanko
AU  - Aleksić-Kovačević, Sanja
AU  - Krnjaić, Dejan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1330
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia
T1  - Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji
VL  - 73
IS  - 10
SP  - 980
EP  - 982
DO  - 10.2298/VSP160809222B
ER  - 

@article{
author = "Bošnjak, Ivan and Zdravković, Nemanja and Čolović, Svetlana and Ranđelović, Slađana and Galić, Nataša and Radojičić, Marina and Šekler, Milanko and Aleksić-Kovačević, Sanja and Krnjaić, Dejan",
year = "2016",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia, Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji",
volume = "73",
number = "10",
pages = "980-982",
doi = "10.2298/VSP160809222B"
}

Bošnjak, I., Zdravković, N., Čolović, S., Ranđelović, S., Galić, N., Radojičić, M., Šekler, M., Aleksić-Kovačević, S.,& Krnjaić, D.. (2016). Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(10), 980-982.
https://doi.org/10.2298/VSP160809222B

Bošnjak I, Zdravković N, Čolović S, Ranđelović S, Galić N, Radojičić M, Šekler M, Aleksić-Kovačević S, Krnjaić D. Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled. 2016;73(10):980-982.
doi:10.2298/VSP160809222B .

Bošnjak, Ivan, Zdravković, Nemanja, Čolović, Svetlana, Ranđelović, Slađana, Galić, Nataša, Radojičić, Marina, Šekler, Milanko, Aleksić-Kovačević, Sanja, Krnjaić, Dejan, "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia" in Vojnosanitetski pregled, 73, no. 10 (2016):980-982,
https://doi.org/10.2298/VSP160809222B . .

TY  - JOUR
AU  - Radojičić, Marina
AU  - Marković, Maja
AU  - Nišavić, Jakov
AU  - Krnjaić, Dejan
AU  - Zdravković, Nemanja
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1334
AB  - Introduction. The objective of our research was to point to the significance of serological diagnostic methods, that is, competitive immunoenzyme test - cELISA as well as the method of classical agglutination of O and H salmonella antigen, for specific antibodies against Salmonella enterica subspecies enterica serovar Enteritidis presence and titre detection in blood serum samples of bothvaccinated and unvaccinated poultry. Material and methods. In our work, we have used commercial competitive immunoenzyme test - cELISA and classical agglutination method with O and H antigens of Salmonella enterica subspecies enterica serovar Enteritidis. Comparative testing included 177 blood serum samples of poultry, out of which 137 was from unvaccinated and 40 from vaccinated individuals originating from majority of poutry farms. Results. In 74 blood serum samples, that is 54,01% out of the total of tested samples originating from unvaccinated flocks, by the use of cELISA test, there were found specific antibodies against S. Enteritidis, while by the method of classical agglutination specific antibodies against O antigen 1,9 and 12 were found in 58 samples, that is in 42,34%, and specific antibodies against H antigen g and m were found in 61 samples, what was 44,53% . In all the tested blood serum samples of vaccinated poultry, there was determined the presence of specific antibodies against S. Enteritidis, both by the use of competitive cELISA method and classical agglutination with somatic O and flagellar H antigens. By the statistical analysis of the results obtained by the method of classical agglutination and cELISA use of kappa test, there was found out a very good compliance (kappa=0,813). Conclusion. Based on the compared results of blood serum testing on the presence of specific antibodies against S. Enteritidis, it can be concluded that cELISA and classic agglutination with O and H antigen methods have a significant place in serological diagnostics of poultry samonelosis, because their application enables detection of the titre of specific antibodies against S. Enteritidis presence in the population of unvaccinated poultry sensitized with antigens of the mentioned causative agent, as well as in unvaccinated animals.
AB  - Uvod. Naša ispitivanja su imala za cilj da ukažu na opravdanost primene seroloških dijagnostičkih metoda, odnosno kompetitivnog imunoenzimskog testa - cELISA i metode klasične aglutinacije O i H antigena salmonela za utvrđivanje prisustva i titra specifičnih antitela protiv Salmonella enterica subspecies enterica serovar Enteritidis u uzorcima krvnog seruma vakcinisane i nevakcinisane živine. Materijal i metode. U radu su korišćeni komercjalni kompetitivni imunoenzimski test - cELISA i metoda klasične aglutinacije korišćenjem O i H antigena Salmonella enterica subspecies enterica serovar Enteritidis. Uporednim ispitivanjem obuhvaćeno je ukupno 177 uzoraka krvnog seruma živine poreklom od 137 nevakcinisanih i 40 vakcinisanih jedinki sa većeg broja živinarskih farmi. Rezultati. Kod 74 uzorka krvnog seruma, odnosno 54,01% od ukupnog broja ispitanih uzoraka seruma živine iz nevakcinisanih jata, primenom cELISA ustanovljena su specifična antitela protiv S. Enteritidis, dok su metodom klasične aglutinacje specifična antitela protiv O antigena 1,9 i 12 utvrđena kod 58 uzoraka, tj. kod 42,34% ispitanih uzoraka, a specifična antitela protiv H antigena g i m ustanovljena kod 61 uzorka, što iznosi 44,53% od ukupnog broja ispitanih uzoraka poreklom od nevakcinisanih jedinki. U svim ispitivanim uzorcima krvnih seruma vakcinisane živine utvrđeno je prisustvo specifičnih antitela protiv S. Enteritidis i to kako primenom kompetitivne cELISA metode tako i klasičnom aglutinacjom sa somatskim O i flagelarnim H antigenima. Statističkom analizom rezultata dobijenih metodom klasične aglutinacije i cELISA primenom kappa testa ustanovljeno je vrlo dobro slaganje (kappa=0,813). Zaključak. Na osnovu poređenja rezultata ispitivanja uzoraka krvnog seruma na prisustvo specifičnih antitela protiv S. Enteritidis, može se zaključiti da metode cELISA i klasična aglutinacija sa O i H antigenima zauzimaju značajno mesto u serološkoj dijagnostici salmoneloze živine jer se njihovom primenom omogućava otkrivanje prisustva i titra specifičnih antitela protiv S. Enteritidis u populaciji nevakcinisane živine senzibilisane antigenima navedenog uzročnika kao i kod vakcinisanih životinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Examining the presence of specific antibodies against Salmonella Enteritidis in vaccinated and unvaccinated poultry
T1  - Issledovanie na naličie specifičeskih antitel k Salmonella Enteritidis u vakcinirovannoj i nevakcinirovannoj domašnej pticy
T1  - Ispitivanje prisustva specifičnih antitela prema Salmonella Enteritidis kod vakcinisane i nevakcinisane živine
VL  - 70
IS  - 1-2
SP  - 3
EP  - 12
DO  - 10.2298/VETGL1602003R
ER  - 

@article{
author = "Radojičić, Marina and Marković, Maja and Nišavić, Jakov and Krnjaić, Dejan and Zdravković, Nemanja",
year = "2016",
abstract = "Introduction. The objective of our research was to point to the significance of serological diagnostic methods, that is, competitive immunoenzyme test - cELISA as well as the method of classical agglutination of O and H salmonella antigen, for specific antibodies against Salmonella enterica subspecies enterica serovar Enteritidis presence and titre detection in blood serum samples of bothvaccinated and unvaccinated poultry. Material and methods. In our work, we have used commercial competitive immunoenzyme test - cELISA and classical agglutination method with O and H antigens of Salmonella enterica subspecies enterica serovar Enteritidis. Comparative testing included 177 blood serum samples of poultry, out of which 137 was from unvaccinated and 40 from vaccinated individuals originating from majority of poutry farms. Results. In 74 blood serum samples, that is 54,01% out of the total of tested samples originating from unvaccinated flocks, by the use of cELISA test, there were found specific antibodies against S. Enteritidis, while by the method of classical agglutination specific antibodies against O antigen 1,9 and 12 were found in 58 samples, that is in 42,34%, and specific antibodies against H antigen g and m were found in 61 samples, what was 44,53% . In all the tested blood serum samples of vaccinated poultry, there was determined the presence of specific antibodies against S. Enteritidis, both by the use of competitive cELISA method and classical agglutination with somatic O and flagellar H antigens. By the statistical analysis of the results obtained by the method of classical agglutination and cELISA use of kappa test, there was found out a very good compliance (kappa=0,813). Conclusion. Based on the compared results of blood serum testing on the presence of specific antibodies against S. Enteritidis, it can be concluded that cELISA and classic agglutination with O and H antigen methods have a significant place in serological diagnostics of poultry samonelosis, because their application enables detection of the titre of specific antibodies against S. Enteritidis presence in the population of unvaccinated poultry sensitized with antigens of the mentioned causative agent, as well as in unvaccinated animals., Uvod. Naša ispitivanja su imala za cilj da ukažu na opravdanost primene seroloških dijagnostičkih metoda, odnosno kompetitivnog imunoenzimskog testa - cELISA i metode klasične aglutinacije O i H antigena salmonela za utvrđivanje prisustva i titra specifičnih antitela protiv Salmonella enterica subspecies enterica serovar Enteritidis u uzorcima krvnog seruma vakcinisane i nevakcinisane živine. Materijal i metode. U radu su korišćeni komercjalni kompetitivni imunoenzimski test - cELISA i metoda klasične aglutinacije korišćenjem O i H antigena Salmonella enterica subspecies enterica serovar Enteritidis. Uporednim ispitivanjem obuhvaćeno je ukupno 177 uzoraka krvnog seruma živine poreklom od 137 nevakcinisanih i 40 vakcinisanih jedinki sa većeg broja živinarskih farmi. Rezultati. Kod 74 uzorka krvnog seruma, odnosno 54,01% od ukupnog broja ispitanih uzoraka seruma živine iz nevakcinisanih jata, primenom cELISA ustanovljena su specifična antitela protiv S. Enteritidis, dok su metodom klasične aglutinacje specifična antitela protiv O antigena 1,9 i 12 utvrđena kod 58 uzoraka, tj. kod 42,34% ispitanih uzoraka, a specifična antitela protiv H antigena g i m ustanovljena kod 61 uzorka, što iznosi 44,53% od ukupnog broja ispitanih uzoraka poreklom od nevakcinisanih jedinki. U svim ispitivanim uzorcima krvnih seruma vakcinisane živine utvrđeno je prisustvo specifičnih antitela protiv S. Enteritidis i to kako primenom kompetitivne cELISA metode tako i klasičnom aglutinacjom sa somatskim O i flagelarnim H antigenima. Statističkom analizom rezultata dobijenih metodom klasične aglutinacije i cELISA primenom kappa testa ustanovljeno je vrlo dobro slaganje (kappa=0,813). Zaključak. Na osnovu poređenja rezultata ispitivanja uzoraka krvnog seruma na prisustvo specifičnih antitela protiv S. Enteritidis, može se zaključiti da metode cELISA i klasična aglutinacija sa O i H antigenima zauzimaju značajno mesto u serološkoj dijagnostici salmoneloze živine jer se njihovom primenom omogućava otkrivanje prisustva i titra specifičnih antitela protiv S. Enteritidis u populaciji nevakcinisane živine senzibilisane antigenima navedenog uzročnika kao i kod vakcinisanih životinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Examining the presence of specific antibodies against Salmonella Enteritidis in vaccinated and unvaccinated poultry, Issledovanie na naličie specifičeskih antitel k Salmonella Enteritidis u vakcinirovannoj i nevakcinirovannoj domašnej pticy, Ispitivanje prisustva specifičnih antitela prema Salmonella Enteritidis kod vakcinisane i nevakcinisane živine",
volume = "70",
number = "1-2",
pages = "3-12",
doi = "10.2298/VETGL1602003R"
}

Radojičić, M., Marković, M., Nišavić, J., Krnjaić, D.,& Zdravković, N.. (2016). Examining the presence of specific antibodies against Salmonella Enteritidis in vaccinated and unvaccinated poultry. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1-2), 3-12.
https://doi.org/10.2298/VETGL1602003R

Radojičić M, Marković M, Nišavić J, Krnjaić D, Zdravković N. Examining the presence of specific antibodies against Salmonella Enteritidis in vaccinated and unvaccinated poultry. in Veterinarski Glasnik. 2016;70(1-2):3-12.
doi:10.2298/VETGL1602003R .

Radojičić, Marina, Marković, Maja, Nišavić, Jakov, Krnjaić, Dejan, Zdravković, Nemanja, "Examining the presence of specific antibodies against Salmonella Enteritidis in vaccinated and unvaccinated poultry" in Veterinarski Glasnik, 70, no. 1-2 (2016):3-12,
https://doi.org/10.2298/VETGL1602003R . .

TY  - JOUR
AU  - Marković, Maja
AU  - Radojičić, Marina
AU  - Zdravković, Nemanja
AU  - Lazić, Marko
AU  - Aksentijević, Ksenija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1341
AB  - Although commensals in digestive tract of a large number of fish species, diplomonads represent very significant opportunistic pathogens. For so far unknown reasons, they can proliferate uncontrollably and thus cause changes in the skin and internal organs in aquarium fish. The problem is confusion over nomenclature of the two most important genera: Spironucleus i Hexamita. Aquarium fish species in which there were diagnosed changes in the skin caused by diplomonads were: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. The fish were treated with 250 mg tablets of metronizadole dissolved in water, or metronizadole in a concentration of 6.6 mg per liter of water. The treatment was successful in only 9 out of 45 treated fish. In the others the symptoms reappeared after certain time. It is necessary to determine the prevalence of the infection in aquarium fish in Serbia, and also examine the success of the treatment with metronizadole applied in food or even other possibilities of the treatment.
AB  - Iako komensali u digestivnom sistemu velikog broja vrsta riba, diplomonade predstavljaju veoma značajne oportune uzročnike bolesti. Iz do sada nedovoljno poznatih razloga, mogu da se nekontrolisano razmnožavaju i izazovu promene na koži i unutrašnjim organima kod akvarijumskih riba. Problem predstavlja konfuzija oko nomenklature dvaju najznačajnijih rodova: Spironucleus i Hexamita. Vrste akvarijumskih riba kod kojih su ustanovljene promene na koži prouzrokovane diplomonadama jesu: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. Ribe su tretirane tabletama od 250 mg metronidazola rastvorenim u vodi, odnosno metronidazolom u koncentraciji 6,6 mg na litar vode. Terapija je bila uspešna samo kod 9 od 45 tretiranih riba. Kod ostalih simptomi su se posle izvesnog vremena ponovo pojavljivali. Neophodno je ustanoviti raširenost infekcije kod akvarijumskih riba u Srbiji, a takođe ispitati uspeh lečenja metronidazolom primenjenim u hrani, ili pak druge mogućnosti tretmana.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Diplomonad caused infection in aquarium fish in Serbia
T1  - Infekcija diplomonadama kod akvarijumskih riba u Srbiji
VL  - 70
IS  - 3-4
SP  - 79
EP  - 87
DO  - 10.2298/VETGL1604079M
ER  - 

@article{
author = "Marković, Maja and Radojičić, Marina and Zdravković, Nemanja and Lazić, Marko and Aksentijević, Ksenija",
year = "2016",
abstract = "Although commensals in digestive tract of a large number of fish species, diplomonads represent very significant opportunistic pathogens. For so far unknown reasons, they can proliferate uncontrollably and thus cause changes in the skin and internal organs in aquarium fish. The problem is confusion over nomenclature of the two most important genera: Spironucleus i Hexamita. Aquarium fish species in which there were diagnosed changes in the skin caused by diplomonads were: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. The fish were treated with 250 mg tablets of metronizadole dissolved in water, or metronizadole in a concentration of 6.6 mg per liter of water. The treatment was successful in only 9 out of 45 treated fish. In the others the symptoms reappeared after certain time. It is necessary to determine the prevalence of the infection in aquarium fish in Serbia, and also examine the success of the treatment with metronizadole applied in food or even other possibilities of the treatment., Iako komensali u digestivnom sistemu velikog broja vrsta riba, diplomonade predstavljaju veoma značajne oportune uzročnike bolesti. Iz do sada nedovoljno poznatih razloga, mogu da se nekontrolisano razmnožavaju i izazovu promene na koži i unutrašnjim organima kod akvarijumskih riba. Problem predstavlja konfuzija oko nomenklature dvaju najznačajnijih rodova: Spironucleus i Hexamita. Vrste akvarijumskih riba kod kojih su ustanovljene promene na koži prouzrokovane diplomonadama jesu: Microgeophagus ramirezi, Apistogramma cacatuoides, Apistogramma nijsseni, Symphysodon aequifasciatus, Pterophyllum altum, Archocentrus nigrofasciatus, Pelvicachromis pulcher i Labidochromis caruleus. Ribe su tretirane tabletama od 250 mg metronidazola rastvorenim u vodi, odnosno metronidazolom u koncentraciji 6,6 mg na litar vode. Terapija je bila uspešna samo kod 9 od 45 tretiranih riba. Kod ostalih simptomi su se posle izvesnog vremena ponovo pojavljivali. Neophodno je ustanoviti raširenost infekcije kod akvarijumskih riba u Srbiji, a takođe ispitati uspeh lečenja metronidazolom primenjenim u hrani, ili pak druge mogućnosti tretmana.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Diplomonad caused infection in aquarium fish in Serbia, Infekcija diplomonadama kod akvarijumskih riba u Srbiji",
volume = "70",
number = "3-4",
pages = "79-87",
doi = "10.2298/VETGL1604079M"
}

Marković, M., Radojičić, M., Zdravković, N., Lazić, M.,& Aksentijević, K.. (2016). Diplomonad caused infection in aquarium fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3-4), 79-87.
https://doi.org/10.2298/VETGL1604079M

Marković M, Radojičić M, Zdravković N, Lazić M, Aksentijević K. Diplomonad caused infection in aquarium fish in Serbia. in Veterinarski Glasnik. 2016;70(3-4):79-87.
doi:10.2298/VETGL1604079M .

Marković, Maja, Radojičić, Marina, Zdravković, Nemanja, Lazić, Marko, Aksentijević, Ksenija, "Diplomonad caused infection in aquarium fish in Serbia" in Veterinarski Glasnik, 70, no. 3-4 (2016):79-87,
https://doi.org/10.2298/VETGL1604079M . .

TY  - JOUR
AU  - Marković, Maja
AU  - Ćirković, Miroslav
AU  - Aleksić, Nevenka
AU  - Milošević, Nikolina
AU  - Bjelić-Čabrilo, Olivera
AU  - Ljubojević, Dragana
AU  - Aksentijević, Ksenija
AU  - Radojičić, Marina
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/847
AB  - Posthodiplostomatosis (black spot disease) is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella) and the bighead carp (Aristichthys nobilis) was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned.
AB  - Postodiplostomatoza je oboljenje mlađih kategorija slatkovodnih riba izazvano infekcijom metecerkarijama trematode Posthodiplostomum cuticola. Promene se zapažaju u vidu crnih cista lokalizovanih prvenstveno u koži, subepidermalnom tkivu i na perajima. Najčešće se javlja kod vrsta riba familija Cyprinidae i Cobitidae. Iako je infekcija duži niz godina prisutna u otvorenim vodama, u radu je opisan prvi nalaz infekcije kod riba iz ribnjaka u Srbiji. Postodiplostomatoza je dijagnostikovana kod belog amura (Ctenopharyngodon idella) i sivog tolstolobika (Aristichthys nobilis). Promene su bile karakteristične, makroskopski vidljive kao tamnosiva do crna kružna polja diskoloracije najčešće na perajima. Njihova prosečna veličina iznosila je 946.5±25.19 μm kod belog amura i 944.5±23.50 μm kod tolstolobika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Posthodiplostomatosis in a fishpond in Serbia
T1  - Postodiplostomatoza na ribnjaku u Srbiji
VL  - 62
IS  - 1
SP  - 101
EP  - 109
DO  - 10.2298/AVB1201101M
ER  - 

@article{
author = "Marković, Maja and Ćirković, Miroslav and Aleksić, Nevenka and Milošević, Nikolina and Bjelić-Čabrilo, Olivera and Ljubojević, Dragana and Aksentijević, Ksenija and Radojičić, Marina",
year = "2012",
abstract = "Posthodiplostomatosis (black spot disease) is a disease of young freshwater fish species of families Cyprinidae and Cobitidae, resulting from infection with metacercaria, which is the penultimate developmental stage of Posthodiplostomum cuticola. Metacercaria give rise to black cysts located primarily in the skin, muscles and on the fins. The disease caused by P. cuticola in certain fish species is first detected in a fishpond in Serbia, as presented in the current work, whilst in natural waters it has been existing for many years. Diagnosis of the infection in the grass carp (Ctenopharyngodon idella) and the bighead carp (Aristichthys nobilis) was based on the occurrence of dark discolorations and cysts of the parasite on the fins. Cysts measured approximately 1 mm in diameter, were confirmed by histopathological means. Posthodiplostomatosis occurred in fishponds in which preventive measures were not implemented, i.e. where the nurseries were not dried up and mechanically cleaned., Postodiplostomatoza je oboljenje mlađih kategorija slatkovodnih riba izazvano infekcijom metecerkarijama trematode Posthodiplostomum cuticola. Promene se zapažaju u vidu crnih cista lokalizovanih prvenstveno u koži, subepidermalnom tkivu i na perajima. Najčešće se javlja kod vrsta riba familija Cyprinidae i Cobitidae. Iako je infekcija duži niz godina prisutna u otvorenim vodama, u radu je opisan prvi nalaz infekcije kod riba iz ribnjaka u Srbiji. Postodiplostomatoza je dijagnostikovana kod belog amura (Ctenopharyngodon idella) i sivog tolstolobika (Aristichthys nobilis). Promene su bile karakteristične, makroskopski vidljive kao tamnosiva do crna kružna polja diskoloracije najčešće na perajima. Njihova prosečna veličina iznosila je 946.5±25.19 μm kod belog amura i 944.5±23.50 μm kod tolstolobika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Posthodiplostomatosis in a fishpond in Serbia, Postodiplostomatoza na ribnjaku u Srbiji",
volume = "62",
number = "1",
pages = "101-109",
doi = "10.2298/AVB1201101M"
}

Marković, M., Ćirković, M., Aleksić, N., Milošević, N., Bjelić-Čabrilo, O., Ljubojević, D., Aksentijević, K.,& Radojičić, M.. (2012). Posthodiplostomatosis in a fishpond in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(1), 101-109.
https://doi.org/10.2298/AVB1201101M

Marković M, Ćirković M, Aleksić N, Milošević N, Bjelić-Čabrilo O, Ljubojević D, Aksentijević K, Radojičić M. Posthodiplostomatosis in a fishpond in Serbia. in Acta Veterinaria-Beograd. 2012;62(1):101-109.
doi:10.2298/AVB1201101M .

Marković, Maja, Ćirković, Miroslav, Aleksić, Nevenka, Milošević, Nikolina, Bjelić-Čabrilo, Olivera, Ljubojević, Dragana, Aksentijević, Ksenija, Radojičić, Marina, "Posthodiplostomatosis in a fishpond in Serbia" in Acta Veterinaria-Beograd, 62, no. 1 (2012):101-109,
https://doi.org/10.2298/AVB1201101M . .

TY  - JOUR
AU  - Radojičić, Marina
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Marković, Maja
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/804
AB  - One of the most common causes of salmonellosis of man and poultry is Salmonella Enteritidis which is often found in the digestive system of adult birds. The infected birds do not display any evident clinical symptoms and, at the same time, they excrete the bacteria into the surrounding environment. Studies are carried out by standard microbiological procedures which include the isolation of Salmonella spp. in egg yolks and their serologic typization by agglutination on microplates. Along these methods, studies on the possibility to use an enzyme immunoassay, such as cELISA, in order to detect the presence of specific antibodies on Salmonella Enteritidis in egg yolks are carried out intensively. The presence of specific antibodies for Salmonella Enteritidis is detected in egg yolk samples from vaccinated flocks resulted in specific positive for a total of 72.22%. Egg yolk samples originating from hens of an unknown immunologic status were cELISA positive in a total of 1.66%. However, egg yolk samples from non-vaccinated hens were positive on the presence of specific antibodies for Salmonella Enteritidis in 23.07% cases. Bearing in mind that standard bacteriological methods did not confirm the presence of Salmonella Enteritidis in egg yolk samples and that cELISA did establish the presence of specific antibodies in the tested samples it can be concluded that cELISA is a more sensitive test.
AB  - Jedan od najčešćih uzročnika salmoneloze ljudi i živine je Salmonella Enteritidis. Pomenuti uzročnik se često nalazi u digestivnom sistemu odrasle živine koja ne pokazuje klinički manifestne simptome oboljenja odakle se izlučuje u spoljašnu sredinu fecesom. Ispitivanja se sprovode kako primenom standardnih metoda bakteriološke dijagnostike koje obuhvataju izolaciju Salmonella spp. iz žumanceta kokošijeg jajeta i njihovu serološku tipizaciju metodom aglutinacije na pločici. Takođe se pored klasične aglutinacije u mikrotitracionim pločama vrše ispitivanja imunoenzimskom probom - ELISA, radi otkrivanja prisustva specifičnih antitela na Salmonella Enteritidis u uzorcima žumanceta jaja kokoši nosilja. Naša ispitivanja su imala za cilj da provere valjanost kompetitivne imunoenzimske probe - cELISA za utvrđivanje prisustva specifičnih antitela protiv navedenog uzročnika u uzorcima žumanaca jaja poreklom od kokoši nosilja. Prisustvo specifičnih antitela za Salmonella Enteritidis ustanovljeno je primenom cELISA kod 72,22% ispitanih uzoraka žumanaca jaja poreklom od vakcinisanih jata i kod 1,66% uzoraka poreklom iz jata nepoznatog imunološkog statusa. Kod 23,07% ispitivanih uzoraka žumanaca jaja poreklom od nevakcinisanih jata ustanovljeno je prisustvo specifičnih antitela za Salmonella Enteritidis primenom metode cELISA. S obzirom da klasičnim bakteriološkim metodama izolacije nije ustanovljeno prisustvo Salmonella Enteritidis u ispitivanim uzorcima, a da je primenom metode cELISA utvrđeno prisustvo specifičnih antitela, može se zaključiti da je cELISA osetljivija dijagnostička metoda.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method
T1  - Ispitivanje prisustva specifičnih antitela na Salmonella Enteritidis u žumancetu jajeta kokoši nosilja kompetitivnom cELISA metodom
VL  - 61
IS  - 2-3
SP  - 205
EP  - 214
DO  - 10.2298/AVB1103205R
ER  - 

@article{
author = "Radojičić, Marina and Milić, Nenad and Nišavić, Jakov and Marković, Maja",
year = "2011",
abstract = "One of the most common causes of salmonellosis of man and poultry is Salmonella Enteritidis which is often found in the digestive system of adult birds. The infected birds do not display any evident clinical symptoms and, at the same time, they excrete the bacteria into the surrounding environment. Studies are carried out by standard microbiological procedures which include the isolation of Salmonella spp. in egg yolks and their serologic typization by agglutination on microplates. Along these methods, studies on the possibility to use an enzyme immunoassay, such as cELISA, in order to detect the presence of specific antibodies on Salmonella Enteritidis in egg yolks are carried out intensively. The presence of specific antibodies for Salmonella Enteritidis is detected in egg yolk samples from vaccinated flocks resulted in specific positive for a total of 72.22%. Egg yolk samples originating from hens of an unknown immunologic status were cELISA positive in a total of 1.66%. However, egg yolk samples from non-vaccinated hens were positive on the presence of specific antibodies for Salmonella Enteritidis in 23.07% cases. Bearing in mind that standard bacteriological methods did not confirm the presence of Salmonella Enteritidis in egg yolk samples and that cELISA did establish the presence of specific antibodies in the tested samples it can be concluded that cELISA is a more sensitive test., Jedan od najčešćih uzročnika salmoneloze ljudi i živine je Salmonella Enteritidis. Pomenuti uzročnik se često nalazi u digestivnom sistemu odrasle živine koja ne pokazuje klinički manifestne simptome oboljenja odakle se izlučuje u spoljašnu sredinu fecesom. Ispitivanja se sprovode kako primenom standardnih metoda bakteriološke dijagnostike koje obuhvataju izolaciju Salmonella spp. iz žumanceta kokošijeg jajeta i njihovu serološku tipizaciju metodom aglutinacije na pločici. Takođe se pored klasične aglutinacije u mikrotitracionim pločama vrše ispitivanja imunoenzimskom probom - ELISA, radi otkrivanja prisustva specifičnih antitela na Salmonella Enteritidis u uzorcima žumanceta jaja kokoši nosilja. Naša ispitivanja su imala za cilj da provere valjanost kompetitivne imunoenzimske probe - cELISA za utvrđivanje prisustva specifičnih antitela protiv navedenog uzročnika u uzorcima žumanaca jaja poreklom od kokoši nosilja. Prisustvo specifičnih antitela za Salmonella Enteritidis ustanovljeno je primenom cELISA kod 72,22% ispitanih uzoraka žumanaca jaja poreklom od vakcinisanih jata i kod 1,66% uzoraka poreklom iz jata nepoznatog imunološkog statusa. Kod 23,07% ispitivanih uzoraka žumanaca jaja poreklom od nevakcinisanih jata ustanovljeno je prisustvo specifičnih antitela za Salmonella Enteritidis primenom metode cELISA. S obzirom da klasičnim bakteriološkim metodama izolacije nije ustanovljeno prisustvo Salmonella Enteritidis u ispitivanim uzorcima, a da je primenom metode cELISA utvrđeno prisustvo specifičnih antitela, može se zaključiti da je cELISA osetljivija dijagnostička metoda.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method, Ispitivanje prisustva specifičnih antitela na Salmonella Enteritidis u žumancetu jajeta kokoši nosilja kompetitivnom cELISA metodom",
volume = "61",
number = "2-3",
pages = "205-214",
doi = "10.2298/AVB1103205R"
}

Radojičić, M., Milić, N., Nišavić, J.,& Marković, M.. (2011). Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 205-214.
https://doi.org/10.2298/AVB1103205R

Radojičić M, Milić N, Nišavić J, Marković M. Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method. in Acta Veterinaria-Beograd. 2011;61(2-3):205-214.
doi:10.2298/AVB1103205R .

Radojičić, Marina, Milić, Nenad, Nišavić, Jakov, Marković, Maja, "Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method" in Acta Veterinaria-Beograd, 61, no. 2-3 (2011):205-214,
https://doi.org/10.2298/AVB1103205R . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Šekler, Marina
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/580
AB  - Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.
AB  - Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry
T1  - Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine
VL  - 63
IS  - 1-2
SP  - 37
EP  - 44
DO  - 10.2298/VETGL0902037M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Radojičić, Marina and Šekler, Marina and Matović, Kazimir and Vidanović, Dejan",
year = "2009",
abstract = "Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32., Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry, Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine",
volume = "63",
number = "1-2",
pages = "37-44",
doi = "10.2298/VETGL0902037M"
}

Milić, N., Nišavić, J., Radojičić, M., Šekler, M., Matović, K.,& Vidanović, D.. (2009). Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 37-44.
https://doi.org/10.2298/VETGL0902037M

Milić N, Nišavić J, Radojičić M, Šekler M, Matović K, Vidanović D. Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik. 2009;63(1-2):37-44.
doi:10.2298/VETGL0902037M .

Milić, Nenad, Nišavić, Jakov, Radojičić, Marina, Šekler, Marina, Matović, Kazimir, Vidanović, Dejan, "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry" in Veterinarski Glasnik, 63, no. 1-2 (2009):37-44,
https://doi.org/10.2298/VETGL0902037M . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Milić, Nenad
AU  - Stojić, Velibor
AU  - Knežević, Dejan
AU  - Vujanac, Ivan
AU  - Dimitrijević, Blagoje
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/648
AB  - A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples.
AB  - Ukupno je ispitano 92 uzorka krvnog seruma junadi na prisustvo antitela protiv goveđeg respiratornog sincicijalnog virusa primenom indirektne imunoenzimske probe - iELISA. Kod 46, odnosno 50% uzoraka krvnog seruma ustanovljena su specifična antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV). Ispitivanjem 92 uzorka krvnog seruma goveda na prisustvo antitela protiv virusa parainfluence 3 (PI 3) ustanovljeno je njihovo prisustvo kod 77, odnosno 83,69% uzoraka, a prisustvo antitela protiv goveđeg herpesvirusa 1 (BHV 1) ustanovljeno je kod 19, odnosno 20,65% uzoraka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe
T1  - Utvrđivanje prisustva antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV), virusa parainfluence 3 (PI3) i goveđeg herpesvirusa 1 (BHV 1) u krvnom serumu junadi primenom indirektne imunoenzimske probe
VL  - 63
IS  - 3-4
SP  - 145
EP  - 152
DO  - 10.2298/VETGL0904145S
ER  - 

@article{
author = "Šamanc, Horea and Milić, Nenad and Stojić, Velibor and Knežević, Dejan and Vujanac, Ivan and Dimitrijević, Blagoje and Nišavić, Jakov and Radojičić, Marina",
year = "2009",
abstract = "A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples., Ukupno je ispitano 92 uzorka krvnog seruma junadi na prisustvo antitela protiv goveđeg respiratornog sincicijalnog virusa primenom indirektne imunoenzimske probe - iELISA. Kod 46, odnosno 50% uzoraka krvnog seruma ustanovljena su specifična antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV). Ispitivanjem 92 uzorka krvnog seruma goveda na prisustvo antitela protiv virusa parainfluence 3 (PI 3) ustanovljeno je njihovo prisustvo kod 77, odnosno 83,69% uzoraka, a prisustvo antitela protiv goveđeg herpesvirusa 1 (BHV 1) ustanovljeno je kod 19, odnosno 20,65% uzoraka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe, Utvrđivanje prisustva antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV), virusa parainfluence 3 (PI3) i goveđeg herpesvirusa 1 (BHV 1) u krvnom serumu junadi primenom indirektne imunoenzimske probe",
volume = "63",
number = "3-4",
pages = "145-152",
doi = "10.2298/VETGL0904145S"
}

Šamanc, H., Milić, N., Stojić, V., Knežević, D., Vujanac, I., Dimitrijević, B., Nišavić, J.,& Radojičić, M.. (2009). Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(3-4), 145-152.
https://doi.org/10.2298/VETGL0904145S

Šamanc H, Milić N, Stojić V, Knežević D, Vujanac I, Dimitrijević B, Nišavić J, Radojičić M. Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe. in Veterinarski Glasnik. 2009;63(3-4):145-152.
doi:10.2298/VETGL0904145S .

Šamanc, Horea, Milić, Nenad, Stojić, Velibor, Knežević, Dejan, Vujanac, Ivan, Dimitrijević, Blagoje, Nišavić, Jakov, Radojičić, Marina, "Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe" in Veterinarski Glasnik, 63, no. 3-4 (2009):145-152,
https://doi.org/10.2298/VETGL0904145S . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Gađanski-Omerović, Gordana
AU  - Ašanin, Ružica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/256
AB  - The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera.
AB  - Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro
T1  - Ispitivanje antigenske strukture i nekih bioloških aktivnosti HN i F antigena virusa Parainfluence 3, in vitro
VL  - 53
IS  - 5-6
SP  - 321
EP  - 332
DO  - 10.2298/AVB0306321M
ER  - 

@article{
author = "Milić, Nenad and Gađanski-Omerović, Gordana and Ašanin, Ružica and Nišavić, Jakov and Radojičić, Marina",
year = "2003",
abstract = "The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera., Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro, Ispitivanje antigenske strukture i nekih bioloških aktivnosti HN i F antigena virusa Parainfluence 3, in vitro",
volume = "53",
number = "5-6",
pages = "321-332",
doi = "10.2298/AVB0306321M"
}

Milić, N., Gađanski-Omerović, G., Ašanin, R., Nišavić, J.,& Radojičić, M.. (2003). Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 321-332.
https://doi.org/10.2298/AVB0306321M

Milić N, Gađanski-Omerović G, Ašanin R, Nišavić J, Radojičić M. Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro. in Acta Veterinaria-Beograd. 2003;53(5-6):321-332.
doi:10.2298/AVB0306321M .

Milić, Nenad, Gađanski-Omerović, Gordana, Ašanin, Ružica, Nišavić, Jakov, Radojičić, Marina, "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):321-332,
https://doi.org/10.2298/AVB0306321M . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Krnjaić, Dejan
AU  - Ašanin, Ružica
AU  - Plavšić, Budimir
AU  - Radojičić, Marina
PY  - 2001
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/160
AB  - Salmonellosis is an important zoonosis manifested in very different clinical picture including enteritis, septicemia, abortion, and combination of disease syndromes. Only 10 to 12 serovars of the Salmonella spp. are capable to causing disease in cattle including Salmonella dublin, S.typhimurium and S. bovismorbificans. In contrast to most other Salmonella serovars, S.dublin shows a high degree of host specificity for cattle. Infection of cattle with S.dublin may be inapparent with carrier state and disease tends to become endemic on certain farms. .
AB  - Salmoneloza je važna zoonoza sa veoma različitom kliničkom slikom koja manifestuje enteritisom, septikemijom, abortusima i kombinovanim simptomima bolesti. Samo 10 do 12 serovarijeteta Salmonella spp. dovodi do infekcije goveda uključujući Salmonella dublin, S.typhimurium and S. bovismorbificans. .
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Infections of cattle caused by Salmonella spp.
T1  - Infekcije goveda izazvane Salmonella vrstama
VL  - 7
IS  - 1
SP  - 329
EP  - 332
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_160
ER  - 

@article{
author = "Mišić, Dušan and Krnjaić, Dejan and Ašanin, Ružica and Plavšić, Budimir and Radojičić, Marina",
year = "2001",
abstract = "Salmonellosis is an important zoonosis manifested in very different clinical picture including enteritis, septicemia, abortion, and combination of disease syndromes. Only 10 to 12 serovars of the Salmonella spp. are capable to causing disease in cattle including Salmonella dublin, S.typhimurium and S. bovismorbificans. In contrast to most other Salmonella serovars, S.dublin shows a high degree of host specificity for cattle. Infection of cattle with S.dublin may be inapparent with carrier state and disease tends to become endemic on certain farms. ., Salmoneloza je važna zoonoza sa veoma različitom kliničkom slikom koja manifestuje enteritisom, septikemijom, abortusima i kombinovanim simptomima bolesti. Samo 10 do 12 serovarijeteta Salmonella spp. dovodi do infekcije goveda uključujući Salmonella dublin, S.typhimurium and S. bovismorbificans. .",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Infections of cattle caused by Salmonella spp., Infekcije goveda izazvane Salmonella vrstama",
volume = "7",
number = "1",
pages = "329-332",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_160"
}

Mišić, D., Krnjaić, D., Ašanin, R., Plavšić, B.,& Radojičić, M.. (2001). Infections of cattle caused by Salmonella spp.. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 7(1), 329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_160

Mišić D, Krnjaić D, Ašanin R, Plavšić B, Radojičić M. Infections of cattle caused by Salmonella spp.. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2001;7(1):329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_160 .

Mišić, Dušan, Krnjaić, Dejan, Ašanin, Ružica, Plavšić, Budimir, Radojičić, Marina, "Infections of cattle caused by Salmonella spp." in Zbornik naučnih radova Instituta PKB Agroekonomik, 7, no. 1 (2001):329-332,
https://hdl.handle.net/21.15107/rcub_veterinar_160 .

TY  - JOUR
AU  - Milić, Nenad
AU  - Gađanski-Omerović, Gordana
AU  - Nišavić, Jakov
AU  - Ašanin, Ružica
AU  - Radojičić, Marina
PY  - 2001
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/187
AB  - Objective of our study was examination of the antigenic structure, hemagglutinating and hemolytic activities of the surface glycoprotein HN and F antigens of purified ND viruses ( molecular weights of 63-65kD and 38-40kD) and glycoprotein subunits isolated from their outer envelopes (molecular weights of 62-64kD and 37-39kD) , activated with 0.025g/dl trypsin-versen, in vitro. The samples of activated purified virions and isolated glycoprotein subunits of ND virus with total protein concentrations of 0.23 and 0.16mg/ml and hemagglutinating titre of 256HAU/0.1ml, induced a hemolysis of chickens erythrocytes at the antigen dilutions of 1:64 and 1:128 per ml of the sample and expressed intensive hemagglutinating activities of 512 and 1024HAU/0.1ml, after their activation with trypsin-versen. After treatment of the aforementioned samples with specific hyperimmune sera against ND virus in which the hemagglutination-inhibition (HI) titre was 1:64, hemolytic activities were not registered. The results show that there are possibilities to use hemolytic test for the fast detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific hyperimmune sera.
AB  - Cilj naših istraživanja je bilo ispitivanje antigenske strukture, hemaglutinacionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa NDV (molekulske težine od 63-65kDa i 38-40kDa) i glikoproteinskih subjedinica izolovanih iz njihovih spoljašnjih omotača (molekulske težine od 62-64kDa i 37-39kDa), aktivisanih sa 0,025g/dl tripsin-versenom, in vitro. Uzorci prečišćenih viriona i izolovanih glikoproteinskih subjedinica virusa NDV sa ukupnim koncentracijama proteina od 0,23 i 0,16mg/ml i hemaglutinacionog titra od 256 HJ/0,1ml, indukovali su posle aktivisanja tripsin-versenom hemolizu pilećih eritrocita u razređenjima antigena 1:64 i 1:128 po ml uzorka i ispoljili intenzivnu hemaglutinacionu aktivnost od 512 i 1024HJ/0,1ml . Posle tretiranja navedenih uzoraka specifičnim hiperimunim serumom protiv virusa NDV čiji je titar bio 1:64, nije ustanovljena njihova hemolitička aktivnost. Rezultati pokazuju da postoji mogućnost korišćenja hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa NDV i njihovu identifikaciju sa specifičnim hiperimunim serumom.
PB  - Unija bioloških naučnih društava Jugoslavije, Beograd
T2  - Acta biologica iugoslavica - serija B: Mikrobiologija
T1  - Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (HN) and fusion (F) glycoprotein antigens of Newcastle disease virus, in vitro
T1  - Ispitivanje antigenske strukture i nekih bioloških aktivnosti hemaglutininsko-neuraminidaznih (HN) i fuzionih (F) glikoproteinskih antigena virusa Newcastle bolesti, in vitro
VL  - 38
IS  - 2
SP  - 45
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_187
ER  - 

@article{
author = "Milić, Nenad and Gađanski-Omerović, Gordana and Nišavić, Jakov and Ašanin, Ružica and Radojičić, Marina",
year = "2001",
abstract = "Objective of our study was examination of the antigenic structure, hemagglutinating and hemolytic activities of the surface glycoprotein HN and F antigens of purified ND viruses ( molecular weights of 63-65kD and 38-40kD) and glycoprotein subunits isolated from their outer envelopes (molecular weights of 62-64kD and 37-39kD) , activated with 0.025g/dl trypsin-versen, in vitro. The samples of activated purified virions and isolated glycoprotein subunits of ND virus with total protein concentrations of 0.23 and 0.16mg/ml and hemagglutinating titre of 256HAU/0.1ml, induced a hemolysis of chickens erythrocytes at the antigen dilutions of 1:64 and 1:128 per ml of the sample and expressed intensive hemagglutinating activities of 512 and 1024HAU/0.1ml, after their activation with trypsin-versen. After treatment of the aforementioned samples with specific hyperimmune sera against ND virus in which the hemagglutination-inhibition (HI) titre was 1:64, hemolytic activities were not registered. The results show that there are possibilities to use hemolytic test for the fast detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific hyperimmune sera., Cilj naših istraživanja je bilo ispitivanje antigenske strukture, hemaglutinacionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa NDV (molekulske težine od 63-65kDa i 38-40kDa) i glikoproteinskih subjedinica izolovanih iz njihovih spoljašnjih omotača (molekulske težine od 62-64kDa i 37-39kDa), aktivisanih sa 0,025g/dl tripsin-versenom, in vitro. Uzorci prečišćenih viriona i izolovanih glikoproteinskih subjedinica virusa NDV sa ukupnim koncentracijama proteina od 0,23 i 0,16mg/ml i hemaglutinacionog titra od 256 HJ/0,1ml, indukovali su posle aktivisanja tripsin-versenom hemolizu pilećih eritrocita u razređenjima antigena 1:64 i 1:128 po ml uzorka i ispoljili intenzivnu hemaglutinacionu aktivnost od 512 i 1024HJ/0,1ml . Posle tretiranja navedenih uzoraka specifičnim hiperimunim serumom protiv virusa NDV čiji je titar bio 1:64, nije ustanovljena njihova hemolitička aktivnost. Rezultati pokazuju da postoji mogućnost korišćenja hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa NDV i njihovu identifikaciju sa specifičnim hiperimunim serumom.",
publisher = "Unija bioloških naučnih društava Jugoslavije, Beograd",
journal = "Acta biologica iugoslavica - serija B: Mikrobiologija",
title = "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (HN) and fusion (F) glycoprotein antigens of Newcastle disease virus, in vitro, Ispitivanje antigenske strukture i nekih bioloških aktivnosti hemaglutininsko-neuraminidaznih (HN) i fuzionih (F) glikoproteinskih antigena virusa Newcastle bolesti, in vitro",
volume = "38",
number = "2",
pages = "45-54",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_187"
}

Milić, N., Gađanski-Omerović, G., Nišavić, J., Ašanin, R.,& Radojičić, M.. (2001). Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (HN) and fusion (F) glycoprotein antigens of Newcastle disease virus, in vitro. in Acta biologica iugoslavica - serija B: Mikrobiologija
Unija bioloških naučnih društava Jugoslavije, Beograd., 38(2), 45-54.
https://hdl.handle.net/21.15107/rcub_veterinar_187

Milić N, Gađanski-Omerović G, Nišavić J, Ašanin R, Radojičić M. Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (HN) and fusion (F) glycoprotein antigens of Newcastle disease virus, in vitro. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2001;38(2):45-54.
https://hdl.handle.net/21.15107/rcub_veterinar_187 .

Milić, Nenad, Gađanski-Omerović, Gordana, Nišavić, Jakov, Ašanin, Ružica, Radojičić, Marina, "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (HN) and fusion (F) glycoprotein antigens of Newcastle disease virus, in vitro" in Acta biologica iugoslavica - serija B: Mikrobiologija, 38, no. 2 (2001):45-54,
https://hdl.handle.net/21.15107/rcub_veterinar_187 .