TY  - JOUR
AU  - Tešović, Bojana
AU  - Nišavić, Jakov
AU  - Banović-Đeri, Bojana
AU  - Petrović, Tamaš
AU  - Radalj, Andrea
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Debeljak, Zoran
AU  - Vasković, Nikola
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2520
AB  - West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.
PB  - Elsevier
T2  - Diagnostic Microbiology and Infectious Disease
T1  - Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform
VL  - 105
IS  - 2
SP  - 115852
DO  - 10.1016/j.diagmicrobio.2022.115852
ER  - 

@article{
author = "Tešović, Bojana and Nišavić, Jakov and Banović-Đeri, Bojana and Petrović, Tamaš and Radalj, Andrea and Šekler, Milanko and Matović, Kazimir and Debeljak, Zoran and Vasković, Nikola and Dmitrić, Marko and Vidanović, Dejan",
year = "2023",
abstract = "West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.",
publisher = "Elsevier",
journal = "Diagnostic Microbiology and Infectious Disease",
title = "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform",
volume = "105",
number = "2",
pages = "115852",
doi = "10.1016/j.diagmicrobio.2022.115852"
}

Tešović, B., Nišavić, J., Banović-Đeri, B., Petrović, T., Radalj, A., Šekler, M., Matović, K., Debeljak, Z., Vasković, N., Dmitrić, M.,& Vidanović, D.. (2023). Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease
Elsevier., 105(2), 115852.
https://doi.org/10.1016/j.diagmicrobio.2022.115852

Tešović B, Nišavić J, Banović-Đeri B, Petrović T, Radalj A, Šekler M, Matović K, Debeljak Z, Vasković N, Dmitrić M, Vidanović D. Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease. 2023;105(2):115852.
doi:10.1016/j.diagmicrobio.2022.115852 .

Tešović, Bojana, Nišavić, Jakov, Banović-Đeri, Bojana, Petrović, Tamaš, Radalj, Andrea, Šekler, Milanko, Matović, Kazimir, Debeljak, Zoran, Vasković, Nikola, Dmitrić, Marko, Vidanović, Dejan, "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform" in Diagnostic Microbiology and Infectious Disease, 105, no. 2 (2023):115852,
https://doi.org/10.1016/j.diagmicrobio.2022.115852 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Debeljak, Mihailo
AU  - Stojanović, Milovan
AU  - Glavčić, Marko
AU  - Marinković, Darko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3340
AB  - Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp.
AB  - Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Prikaz slučaja leptospiroze kod dve alpake
T1  - Leptospirosis in two alpacas - case report
SP  - 134
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3340
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Debeljak, Mihailo and Stojanović, Milovan and Glavčić, Marko and Marinković, Darko",
year = "2023",
abstract = "Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp., Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Prikaz slučaja leptospiroze kod dve alpake, Leptospirosis in two alpacas - case report",
pages = "134-135",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3340"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Debeljak, M., Stojanović, M., Glavčić, M.,& Marinković, D.. (2023). Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Debeljak M, Stojanović M, Glavčić M, Marinković D. Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Debeljak, Mihailo, Stojanović, Milovan, Glavčić, Marko, Marinković, Darko, "Prikaz slučaja leptospiroze kod dve alpake" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):134-135,
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Petrović, Tamaš
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2706
AB  - In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.
PB  - MDPI
T2  - Animals
T1  - Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
VL  - 13
IS  - 4
SP  - 700
DO  - 10.3390/ani13040700
ER  - 

@article{
author = "Đurđević, Biljana and Polaček, Vladimir and Pajić, Marko and Petrović, Tamaš and Vučićević, Ivana and Vidanović, Dejan and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.",
publisher = "MDPI",
journal = "Animals",
title = "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia",
volume = "13",
number = "4",
pages = "700",
doi = "10.3390/ani13040700"
}

Đurđević, B., Polaček, V., Pajić, M., Petrović, T., Vučićević, I., Vidanović, D.,& Aleksić-Kovačević, S.. (2023). Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals
MDPI., 13(4), 700.
https://doi.org/10.3390/ani13040700

Đurđević B, Polaček V, Pajić M, Petrović T, Vučićević I, Vidanović D, Aleksić-Kovačević S. Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals. 2023;13(4):700.
doi:10.3390/ani13040700 .

Đurđević, Biljana, Polaček, Vladimir, Pajić, Marko, Petrović, Tamaš, Vučićević, Ivana, Vidanović, Dejan, Aleksić-Kovačević, Sanja, "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia" in Animals, 13, no. 4 (2023):700,
https://doi.org/10.3390/ani13040700 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3175
AB  - Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести.
AB  - Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
T1  - Prikaz slučaja Aujeskijeve bolesti kod lovnog psa
T1  - Aujezsky’s disease in a hunting dog - case report
SP  - 52
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3175
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2022",
abstract = "Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести., Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022",
title = "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa, Aujezsky’s disease in a hunting dog - case report",
pages = "52-53",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3175"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2022). Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022. 2022;:52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa" in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022 (2022):52-53,
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

TY  - JOUR
AU  - Marinković, Darko
AU  - Gavrilović, Pavle
AU  - Vidanović, Dejan
AU  - Ćirović, Duško
AU  - Kuručki, Milica
AU  - Vasković, Nikola
AU  - Aničić, Milan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2841
AB  - Background Alveolar hydatid disease caused by the tapeworm Echinococcus multilocularis is a parasitic disease present
in the northern hemisphere. Echinococcus multilocularis is a parasite of canid and felid carnivores as defnitive hosts, and
small mammals, particularly rodents as intermediate hosts. Other animal species and humans can be aberrant intermediate
hosts for this parasite. It is known that besides acting as defnitive hosts, domestic dogs can rarely become infected with the
larval form of E. multilocularis and develop alveolar echinococcosis; however, a role of wild canids as aberrant intermediate
hosts has not been documented until now. To the best of our knowledge the present paper provides the frst description of
alveolar hydatid disease in a golden jackal (Canis aureus).
Case Presentation Necropsy of the yearling female animal found a large, round, tumor-like mass, 20 cm in diameter, with a
rough, multilobulated surface in the abdominal cavity, connected to the liver and omentum. On the cut surface this tumorlike lesion was multicystic, with a number of locular cavities flled with a clear yellowish to orange watery fuid and a large
area of necrosis in the central part of the mass. Histopathology revealed multiple cystic spaces separated by fbrous sheaths
and infammatory cells—lymphocytes, plasma cells, neutrophil and eosinophil granulocytes. The cysts contained either
pale, hyaline, eosinophilic laminar and occasionally amorphous, acellular, PAS-positive structures, or metacestodes with
invaginated protoscolices. In several cysts round calcifed bodies (calcareous corpuscles) were noted. Microscopic examination showed everted and inverted protoscolices which were attached to fragments of the brood capsule or free in hydatid
fuid. By comparing consensus nucleotide sequence of 457 bp obtained by PCR reaction with sequences deposited in NCBI
GenBank it is determined that it was 100% identical with E. multilocularis sequences under accession numbers MH259778.1,
MH259776.1, AB668376.1, EU704124.1 and AB018440.2.
Conclusions The present paper provides a proof that the golden jackal, besides being a defnitive host, can also serve as the
aberrant intermediate host for E. multilocularis.
PB  - Springer
T2  - Acta Parasitologica
T1  - First Report of Alveolar Hydatid Disease (Echinococcus multilocularis) in a Golden Jackal (Canis aureus)
VL  - 67
IS  - 3
SP  - 1401
EP  - 1406
DO  - 10.1007/s11686-022-00556-y
ER  - 

@article{
author = "Marinković, Darko and Gavrilović, Pavle and Vidanović, Dejan and Ćirović, Duško and Kuručki, Milica and Vasković, Nikola and Aničić, Milan",
year = "2022",
abstract = "Background Alveolar hydatid disease caused by the tapeworm Echinococcus multilocularis is a parasitic disease present
in the northern hemisphere. Echinococcus multilocularis is a parasite of canid and felid carnivores as defnitive hosts, and
small mammals, particularly rodents as intermediate hosts. Other animal species and humans can be aberrant intermediate
hosts for this parasite. It is known that besides acting as defnitive hosts, domestic dogs can rarely become infected with the
larval form of E. multilocularis and develop alveolar echinococcosis; however, a role of wild canids as aberrant intermediate
hosts has not been documented until now. To the best of our knowledge the present paper provides the frst description of
alveolar hydatid disease in a golden jackal (Canis aureus).
Case Presentation Necropsy of the yearling female animal found a large, round, tumor-like mass, 20 cm in diameter, with a
rough, multilobulated surface in the abdominal cavity, connected to the liver and omentum. On the cut surface this tumorlike lesion was multicystic, with a number of locular cavities flled with a clear yellowish to orange watery fuid and a large
area of necrosis in the central part of the mass. Histopathology revealed multiple cystic spaces separated by fbrous sheaths
and infammatory cells—lymphocytes, plasma cells, neutrophil and eosinophil granulocytes. The cysts contained either
pale, hyaline, eosinophilic laminar and occasionally amorphous, acellular, PAS-positive structures, or metacestodes with
invaginated protoscolices. In several cysts round calcifed bodies (calcareous corpuscles) were noted. Microscopic examination showed everted and inverted protoscolices which were attached to fragments of the brood capsule or free in hydatid
fuid. By comparing consensus nucleotide sequence of 457 bp obtained by PCR reaction with sequences deposited in NCBI
GenBank it is determined that it was 100% identical with E. multilocularis sequences under accession numbers MH259778.1,
MH259776.1, AB668376.1, EU704124.1 and AB018440.2.
Conclusions The present paper provides a proof that the golden jackal, besides being a defnitive host, can also serve as the
aberrant intermediate host for E. multilocularis.",
publisher = "Springer",
journal = "Acta Parasitologica",
title = "First Report of Alveolar Hydatid Disease (Echinococcus multilocularis) in a Golden Jackal (Canis aureus)",
volume = "67",
number = "3",
pages = "1401-1406",
doi = "10.1007/s11686-022-00556-y"
}

Marinković, D., Gavrilović, P., Vidanović, D., Ćirović, D., Kuručki, M., Vasković, N.,& Aničić, M.. (2022). First Report of Alveolar Hydatid Disease (Echinococcus multilocularis) in a Golden Jackal (Canis aureus). in Acta Parasitologica
Springer., 67(3), 1401-1406.
https://doi.org/10.1007/s11686-022-00556-y

Marinković D, Gavrilović P, Vidanović D, Ćirović D, Kuručki M, Vasković N, Aničić M. First Report of Alveolar Hydatid Disease (Echinococcus multilocularis) in a Golden Jackal (Canis aureus). in Acta Parasitologica. 2022;67(3):1401-1406.
doi:10.1007/s11686-022-00556-y .

Marinković, Darko, Gavrilović, Pavle, Vidanović, Dejan, Ćirović, Duško, Kuručki, Milica, Vasković, Nikola, Aničić, Milan, "First Report of Alveolar Hydatid Disease (Echinococcus multilocularis) in a Golden Jackal (Canis aureus)" in Acta Parasitologica, 67, no. 3 (2022):1401-1406,
https://doi.org/10.1007/s11686-022-00556-y . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2926
AB  - Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса.
AB  - Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
T1  - Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji
T1  - Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2926
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Aničić, Milan and Marinković, Darko",
year = "2021",
abstract = "Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса., Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021",
title = "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji, Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2926"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Aničić, M.,& Marinković, D.. (2021). Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Aničić M, Marinković D. Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021. 2021;:140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Aničić, Milan, Marinković, Darko, "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji" in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021 (2021):140-141,
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Radojičić, Sonja
AU  - Debeljak, Zoran
AU  - Šekler, Milanko
AU  - Ćirić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797
AB  - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
PB  - Elsevier, Amsterdam
T2  - Saudi Journal of Biological Sciences
T1  - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
VL  - 27
IS  - 1
SP  - 518
EP  - 523
DO  - 10.1016/j.sjbs.2019.11.012
ER  - 

@article{
author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena",
year = "2020",
abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Journal of Biological Sciences",
title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia",
volume = "27",
number = "1",
pages = "518-523",
doi = "10.1016/j.sjbs.2019.11.012"
}

Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences
Elsevier, Amsterdam., 27(1), 518-523.
https://doi.org/10.1016/j.sjbs.2019.11.012

Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523.
doi:10.1016/j.sjbs.2019.11.012 .

Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523,
https://doi.org/10.1016/j.sjbs.2019.11.012 . .

TY  - JOUR
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Petrović, Milos
AU  - Nišavić, Jakov
AU  - Bacić, Dragan
AU  - Petrović, Tamaš
AU  - Vidanović, Dejan
AU  - Obrenović, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1792
AB  - Lumpy skin disease (LSD) is an infectious disease of cattle caused by virus of the Capripoxvirus genus (LSDV), family Poxviridae. Until 2015, it had not appeared in the Balkans. In June 2016, LSD spread throughout Serbia. This study analyses the first LSD occurrence, epizootic features, applied diagnostic procedures and control measures in five districts in south-east Serbia (Pcinja, Jablanica, Pirot, Toplica and Nisava). In total, there were 225 LSD outbreaks reported in Serbia, out of which 189 (84%) were located in the study area. The highest number of outbreaks was registered in Pcinja district (169), where LSD was first registered. The median distance and time between the nearest previous outbreak sites were calculated (4.32 km and 9 days). The median altitude of outbreak locations was 992 m with more than 90% above 500 m (p <= 0.001). The average herd morbidity rate in the study area was 13.6% and the herd mortality rate was recorded only in Pcinja (0.5%) and Jablanica (1.6%) districts. Samples taken from the cattle suspected to LSD were subjected to real time PCR analysis. Out of 233 samples tested for LSDV 132 (56.7%) were positive. The LSDV genome was identified in skin nodules (85.4%), blood (72.7%) and nasal swabs (62.5%). Phylogenetic analysis indicated that the LSDV strain circulating in Serbia fell within the cluster of field LSDV found worldwide. In response to the LSD epizootic, animal trade and movement were prohibited, complete stamping out, disinfection, disinsection and an entire bovine population vaccination using the homologous Neethling live attenuated vaccine (OBP, South Africa) were conducted. A month and a half after the completion of the vaccination campaign, the LSD epizootic was stopped, and no new cases have been reported since.
PB  - Wiley, Hoboken
T2  - Transboundary and Emerging Diseases
T1  - Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016
VL  - 66
IS  - 5
SP  - 2087
EP  - 2099
DO  - 10.1111/tbed.13261
ER  - 

@article{
author = "Manić, Marija and Stojiljković, Marko and Petrović, Milos and Nišavić, Jakov and Bacić, Dragan and Petrović, Tamaš and Vidanović, Dejan and Obrenović, Sonja",
year = "2019",
abstract = "Lumpy skin disease (LSD) is an infectious disease of cattle caused by virus of the Capripoxvirus genus (LSDV), family Poxviridae. Until 2015, it had not appeared in the Balkans. In June 2016, LSD spread throughout Serbia. This study analyses the first LSD occurrence, epizootic features, applied diagnostic procedures and control measures in five districts in south-east Serbia (Pcinja, Jablanica, Pirot, Toplica and Nisava). In total, there were 225 LSD outbreaks reported in Serbia, out of which 189 (84%) were located in the study area. The highest number of outbreaks was registered in Pcinja district (169), where LSD was first registered. The median distance and time between the nearest previous outbreak sites were calculated (4.32 km and 9 days). The median altitude of outbreak locations was 992 m with more than 90% above 500 m (p <= 0.001). The average herd morbidity rate in the study area was 13.6% and the herd mortality rate was recorded only in Pcinja (0.5%) and Jablanica (1.6%) districts. Samples taken from the cattle suspected to LSD were subjected to real time PCR analysis. Out of 233 samples tested for LSDV 132 (56.7%) were positive. The LSDV genome was identified in skin nodules (85.4%), blood (72.7%) and nasal swabs (62.5%). Phylogenetic analysis indicated that the LSDV strain circulating in Serbia fell within the cluster of field LSDV found worldwide. In response to the LSD epizootic, animal trade and movement were prohibited, complete stamping out, disinfection, disinsection and an entire bovine population vaccination using the homologous Neethling live attenuated vaccine (OBP, South Africa) were conducted. A month and a half after the completion of the vaccination campaign, the LSD epizootic was stopped, and no new cases have been reported since.",
publisher = "Wiley, Hoboken",
journal = "Transboundary and Emerging Diseases",
title = "Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016",
volume = "66",
number = "5",
pages = "2087-2099",
doi = "10.1111/tbed.13261"
}

Manić, M., Stojiljković, M., Petrović, M., Nišavić, J., Bacić, D., Petrović, T., Vidanović, D.,& Obrenović, S.. (2019). Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016. in Transboundary and Emerging Diseases
Wiley, Hoboken., 66(5), 2087-2099.
https://doi.org/10.1111/tbed.13261

Manić M, Stojiljković M, Petrović M, Nišavić J, Bacić D, Petrović T, Vidanović D, Obrenović S. Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016. in Transboundary and Emerging Diseases. 2019;66(5):2087-2099.
doi:10.1111/tbed.13261 .

Manić, Marija, Stojiljković, Marko, Petrović, Milos, Nišavić, Jakov, Bacić, Dragan, Petrović, Tamaš, Vidanović, Dejan, Obrenović, Sonja, "Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016" in Transboundary and Emerging Diseases, 66, no. 5 (2019):2087-2099,
https://doi.org/10.1111/tbed.13261 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3337
AB  - У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима.
AB  - In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Prikaz slučaja mononuklearnog dermatitisa fazana
T1  - Case report: Mononuclear dermatitis in pheasant
SP  - 164
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3337
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2019",
abstract = "У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима., In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Prikaz slučaja mononuklearnog dermatitisa fazana, Case report: Mononuclear dermatitis in pheasant",
pages = "164-165",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3337"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2019). Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja mononuklearnog dermatitisa fazana" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):164-165,
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

TY  - JOUR
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1697
AB  - Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia
VL  - 69
IS  - 4
SP  - 369
EP  - 378
DO  - 10.2478/acve-2019-0031
ER  - 

@article{
author = "Vasković, Nikola and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Matović, Kazimir and Aničić, Milan and Marinković, Darko",
year = "2019",
abstract = "Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia",
volume = "69",
number = "4",
pages = "369-378",
doi = "10.2478/acve-2019-0031"
}

Vasković, N., Debeljak, Z., Vidanović, D., Šekler, M., Matović, K., Aničić, M.,& Marinković, D.. (2019). Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 369-378.
https://doi.org/10.2478/acve-2019-0031

Vasković N, Debeljak Z, Vidanović D, Šekler M, Matović K, Aničić M, Marinković D. Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd. 2019;69(4):369-378.
doi:10.2478/acve-2019-0031 .

Vasković, Nikola, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Matović, Kazimir, Aničić, Milan, Marinković, Darko, "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia" in Acta Veterinaria-Beograd, 69, no. 4 (2019):369-378,
https://doi.org/10.2478/acve-2019-0031 . .

TY  - JOUR
AU  - Gavrilović, Pavle
AU  - Marinković, Darko
AU  - Vidanović, Dejan
AU  - Dobrosavljević, Ivan
AU  - Gavrilović, Aleksandra
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1753
AB  - Angiostrongylosis caused by Angiostrongylus vasorum is an emerging disease in Europe. Recent reports have shown that, besides the red fox (Vulpes vulpes) recognized as the main definitive host and reservoir for the parasite, the role of the definitive host can be taken by a range of mammals. We considered that, due to the rapid large-scale expansion of its populations in Europe, the golden jackal (Canis aureus) could assume an important role in the epidemiology of the disease. The aim of the investigations was to explore the role of the golden jackal as the definitive host for A. vasorum. Sixty-three golden jackals, legally hunted in lowlands around the Danube River in Serbia recognized as the core area of the species distribution in Europe, were subjected to patho-morphological and parasitological examination. The adult forms of A. vasorum were detected in the pulmonary arteries in six golden jackals with gross lesions manifested in the lungs. The finding of first stage larvae (L1) of A. vasorum in microscopic smears of the lung tissue altered by infection, which was consistent with the presence of adult parasites and manifestation of gross lesions in the lungs, confirms the successful reproduction of the parasite in the golden jackal. Migration of L1 from the blood vessels to the airways was confirmed by histopathology and, subsequently, their shedding was demonstrated by the Baermann method. The results support the hypothesis that the golden jackal acts as a suitable definitive host for A. vasorum. As a definitive host with a large-scale expansion of its populations in Europe, the golden jackal may be an important part of the parasites host repertoire by spreading the parasite into previously non-endemic areas and by being an additional definitive host in endemic areas of vulpine angiostrongylosis.
PB  - Wiley, Hoboken
T2  - Transboundary and Emerging Diseases
T1  - Are golden jackals (Canis aureus) definitive hosts for Angiostrongylus vasorum?
VL  - 66
IS  - 6
SP  - 2305
EP  - 2310
DO  - 10.1111/tbed.13284
ER  - 

@article{
author = "Gavrilović, Pavle and Marinković, Darko and Vidanović, Dejan and Dobrosavljević, Ivan and Gavrilović, Aleksandra",
year = "2019",
abstract = "Angiostrongylosis caused by Angiostrongylus vasorum is an emerging disease in Europe. Recent reports have shown that, besides the red fox (Vulpes vulpes) recognized as the main definitive host and reservoir for the parasite, the role of the definitive host can be taken by a range of mammals. We considered that, due to the rapid large-scale expansion of its populations in Europe, the golden jackal (Canis aureus) could assume an important role in the epidemiology of the disease. The aim of the investigations was to explore the role of the golden jackal as the definitive host for A. vasorum. Sixty-three golden jackals, legally hunted in lowlands around the Danube River in Serbia recognized as the core area of the species distribution in Europe, were subjected to patho-morphological and parasitological examination. The adult forms of A. vasorum were detected in the pulmonary arteries in six golden jackals with gross lesions manifested in the lungs. The finding of first stage larvae (L1) of A. vasorum in microscopic smears of the lung tissue altered by infection, which was consistent with the presence of adult parasites and manifestation of gross lesions in the lungs, confirms the successful reproduction of the parasite in the golden jackal. Migration of L1 from the blood vessels to the airways was confirmed by histopathology and, subsequently, their shedding was demonstrated by the Baermann method. The results support the hypothesis that the golden jackal acts as a suitable definitive host for A. vasorum. As a definitive host with a large-scale expansion of its populations in Europe, the golden jackal may be an important part of the parasites host repertoire by spreading the parasite into previously non-endemic areas and by being an additional definitive host in endemic areas of vulpine angiostrongylosis.",
publisher = "Wiley, Hoboken",
journal = "Transboundary and Emerging Diseases",
title = "Are golden jackals (Canis aureus) definitive hosts for Angiostrongylus vasorum?",
volume = "66",
number = "6",
pages = "2305-2310",
doi = "10.1111/tbed.13284"
}

Gavrilović, P., Marinković, D., Vidanović, D., Dobrosavljević, I.,& Gavrilović, A.. (2019). Are golden jackals (Canis aureus) definitive hosts for Angiostrongylus vasorum?. in Transboundary and Emerging Diseases
Wiley, Hoboken., 66(6), 2305-2310.
https://doi.org/10.1111/tbed.13284

Gavrilović P, Marinković D, Vidanović D, Dobrosavljević I, Gavrilović A. Are golden jackals (Canis aureus) definitive hosts for Angiostrongylus vasorum?. in Transboundary and Emerging Diseases. 2019;66(6):2305-2310.
doi:10.1111/tbed.13284 .

Gavrilović, Pavle, Marinković, Darko, Vidanović, Dejan, Dobrosavljević, Ivan, Gavrilović, Aleksandra, "Are golden jackals (Canis aureus) definitive hosts for Angiostrongylus vasorum?" in Transboundary and Emerging Diseases, 66, no. 6 (2019):2305-2310,
https://doi.org/10.1111/tbed.13284 . .

TY  - CONF
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Sarić, Ljubiša
AU  - Karabasil, Nedjeljko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1677
AB  - The aim of this paper was to evaluate two real-time PCR (qPCR) protocols for the detection of Salmonella spp. in minced meat and chicken neck skin, after DNA extraction using the InstaTM Gene matrix (BioRad, USA) and DNA extraction based on thermal cell lysis. The applied molecular methods were sensitive and specific for the rapid detection of Salmonella spp. in minced meat and chicken neck skin. The qualitative results were identical regardless of the applied DNA extraction or qPCR protocols. Lower Cq values were achieved after DNA extraction using the InstaTM Gene matrix.
PB  - Iop Publishing Ltd, Bristol
C3  - 60th International Meat Industry Conference (MEATCON2019)
T1  - Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures
VL  - 333
SP  - UNSP 012041
DO  - 10.1088/1755-1315/333/1/012041
ER  - 

@conference{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Sarić, Ljubiša and Karabasil, Nedjeljko",
year = "2019",
abstract = "The aim of this paper was to evaluate two real-time PCR (qPCR) protocols for the detection of Salmonella spp. in minced meat and chicken neck skin, after DNA extraction using the InstaTM Gene matrix (BioRad, USA) and DNA extraction based on thermal cell lysis. The applied molecular methods were sensitive and specific for the rapid detection of Salmonella spp. in minced meat and chicken neck skin. The qualitative results were identical regardless of the applied DNA extraction or qPCR protocols. Lower Cq values were achieved after DNA extraction using the InstaTM Gene matrix.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "60th International Meat Industry Conference (MEATCON2019)",
title = "Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures",
volume = "333",
pages = "UNSP 012041",
doi = "10.1088/1755-1315/333/1/012041"
}

Dmitrić, M., Vidanović, D., Matović, K., Sarić, L.,& Karabasil, N.. (2019). Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures. in 60th International Meat Industry Conference (MEATCON2019)
Iop Publishing Ltd, Bristol., 333, UNSP 012041.
https://doi.org/10.1088/1755-1315/333/1/012041

Dmitrić M, Vidanović D, Matović K, Sarić L, Karabasil N. Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures. in 60th International Meat Industry Conference (MEATCON2019). 2019;333:UNSP 012041.
doi:10.1088/1755-1315/333/1/012041 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Sarić, Ljubiša, Karabasil, Nedjeljko, "Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures" in 60th International Meat Industry Conference (MEATCON2019), 333 (2019):UNSP 012041,
https://doi.org/10.1088/1755-1315/333/1/012041 . .

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Grego, Edita
AU  - Vidanović, Dejan
AU  - Milanov, Dubravka
AU  - Krnjaić, Dejan
AU  - Kehrenberg, Corinna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1648
AB  - The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
VL  - 24
IS  - 1
SP  - 95
EP  - 103
DO  - 10.1089/mdr.2017.0016
ER  - 

@article{
author = "Todorović, Dalibor and Velhner, Maja and Grego, Edita and Vidanović, Dejan and Milanov, Dubravka and Krnjaić, Dejan and Kehrenberg, Corinna",
year = "2018",
abstract = "The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia",
volume = "24",
number = "1",
pages = "95-103",
doi = "10.1089/mdr.2017.0016"
}

Todorović, D., Velhner, M., Grego, E., Vidanović, D., Milanov, D., Krnjaić, D.,& Kehrenberg, C.. (2018). Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 24(1), 95-103.
https://doi.org/10.1089/mdr.2017.0016

Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance. 2018;24(1):95-103.
doi:10.1089/mdr.2017.0016 .

Todorović, Dalibor, Velhner, Maja, Grego, Edita, Vidanović, Dejan, Milanov, Dubravka, Krnjaić, Dejan, Kehrenberg, Corinna, "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia" in Microbial Drug Resistance, 24, no. 1 (2018):95-103,
https://doi.org/10.1089/mdr.2017.0016 . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Vidanović, Dejan
AU  - Božić, B.
AU  - Becskei, Zsolt
AU  - Vučićević, Ivana
AU  - Prodanov-Radulović, Jasna
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1598
AB  - The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
PB  - Faculty of Veterinary Medicine - Skopje
T2  - Macedonian Veterinary Review
T1  - The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis
VL  - 41
IS  - 1
SP  - 47
EP  - 53
DO  - 10.1515/macvetrev-2017-0030
ER  - 

@article{
author = "Polaček, Vladimir and Vidanović, Dejan and Božić, B. and Becskei, Zsolt and Vučićević, Ivana and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.",
publisher = "Faculty of Veterinary Medicine - Skopje",
journal = "Macedonian Veterinary Review",
title = "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis",
volume = "41",
number = "1",
pages = "47-53",
doi = "10.1515/macvetrev-2017-0030"
}

Polaček, V., Vidanović, D., Božić, B., Becskei, Z., Vučićević, I., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review
Faculty of Veterinary Medicine - Skopje., 41(1), 47-53.
https://doi.org/10.1515/macvetrev-2017-0030

Polaček V, Vidanović D, Božić B, Becskei Z, Vučićević I, Prodanov-Radulović J, Aleksić-Kovačević S. The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review. 2018;41(1):47-53.
doi:10.1515/macvetrev-2017-0030 .

Polaček, Vladimir, Vidanović, Dejan, Božić, B., Becskei, Zsolt, Vučićević, Ivana, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis" in Macedonian Veterinary Review, 41, no. 1 (2018):47-53,
https://doi.org/10.1515/macvetrev-2017-0030 . .

TY  - JOUR
AU  - Bozić, Biljana
AU  - Polaček, Vladimir
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Vasković, Nikola
AU  - Prodanov-Radulović, Jasna
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1651
AB  - During the epizootic of highly pathogenic avian influenza subtype H5N8 in Serbia in the winter of 2016-2017, the highest percent of mortality due to this infection was recorded in mute swans (Cygnus olor). Besides mute swans, avian influenza virus subtype H5N8 was also diagnosed in a small number of hens in rural households. Pancreatic tissues from avian influenza H5N8 positive mute swans and hens that died during this outbreak were collected to determine the character of morphological lesions and the distribution of the viral antigen in this organ. Macroscopic examination of the pancreas of mute swans revealed hemorrhages as well as necrosis, while there were no macroscopic visible lesions in the pancreas of infected hens. Despite the different macroscopic finding, microscopic examination of the pancreas of both infected bird species revealed lesions in the form of acute pancreatitis and multifocal acinar necrosis. The viral antigen was abundantly expressed in the cytoplasm and nucleus of necrotic cells, as well as in macrophages in both examined bird species. Immunohistochemical expression of the viral antigen in the pancreas was strongly consistent with histological lesions. According to the above descnbed findings, it could be concluded that highly pathogenic avian influenza virus H5N8 has a high affinity to pancreatic tissue in both mute swans and hens and the distribution and the character of the lesions in the pancreas are similar in both bird species.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8
VL  - 68
IS  - 2
SP  - 217
EP  - 223
DO  - 10.2478/acve-2018-0018
ER  - 

@article{
author = "Bozić, Biljana and Polaček, Vladimir and Vučićević, Ivana and Vidanović, Dejan and Vasković, Nikola and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "During the epizootic of highly pathogenic avian influenza subtype H5N8 in Serbia in the winter of 2016-2017, the highest percent of mortality due to this infection was recorded in mute swans (Cygnus olor). Besides mute swans, avian influenza virus subtype H5N8 was also diagnosed in a small number of hens in rural households. Pancreatic tissues from avian influenza H5N8 positive mute swans and hens that died during this outbreak were collected to determine the character of morphological lesions and the distribution of the viral antigen in this organ. Macroscopic examination of the pancreas of mute swans revealed hemorrhages as well as necrosis, while there were no macroscopic visible lesions in the pancreas of infected hens. Despite the different macroscopic finding, microscopic examination of the pancreas of both infected bird species revealed lesions in the form of acute pancreatitis and multifocal acinar necrosis. The viral antigen was abundantly expressed in the cytoplasm and nucleus of necrotic cells, as well as in macrophages in both examined bird species. Immunohistochemical expression of the viral antigen in the pancreas was strongly consistent with histological lesions. According to the above descnbed findings, it could be concluded that highly pathogenic avian influenza virus H5N8 has a high affinity to pancreatic tissue in both mute swans and hens and the distribution and the character of the lesions in the pancreas are similar in both bird species.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8",
volume = "68",
number = "2",
pages = "217-223",
doi = "10.2478/acve-2018-0018"
}

Bozić, B., Polaček, V., Vučićević, I., Vidanović, D., Vasković, N., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(2), 217-223.
https://doi.org/10.2478/acve-2018-0018

Bozić B, Polaček V, Vučićević I, Vidanović D, Vasković N, Prodanov-Radulović J, Aleksić-Kovačević S. Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8. in Acta Veterinaria-Beograd. 2018;68(2):217-223.
doi:10.2478/acve-2018-0018 .

Bozić, Biljana, Polaček, Vladimir, Vučićević, Ivana, Vidanović, Dejan, Vasković, Nikola, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "Morphological differences of pancreatic lesions in mute swans and hens naturally infected with highly pathogenic avian influenza virus h5n8" in Acta Veterinaria-Beograd, 68, no. 2 (2018):217-223,
https://doi.org/10.2478/acve-2018-0018 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1581
AB  - After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.
PB  - Allen Press Inc, Lawrence
T2  - Journal of Parasitology
T1  - Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia
VL  - 104
IS  - 5
SP  - 557
EP  - 559
DO  - 10.1645/18-42
ER  - 

@article{
author = "Dmitrić, Marko and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Vasković, Nikola and Matović, Kazimir and Karabasil, Nedjeljko",
year = "2018",
abstract = "After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.",
publisher = "Allen Press Inc, Lawrence",
journal = "Journal of Parasitology",
title = "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia",
volume = "104",
number = "5",
pages = "557-559",
doi = "10.1645/18-42"
}

Dmitrić, M., Debeljak, Z., Vidanović, D., Šekler, M., Vasković, N., Matović, K.,& Karabasil, N.. (2018). Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology
Allen Press Inc, Lawrence., 104(5), 557-559.
https://doi.org/10.1645/18-42

Dmitrić M, Debeljak Z, Vidanović D, Šekler M, Vasković N, Matović K, Karabasil N. Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology. 2018;104(5):557-559.
doi:10.1645/18-42 .

Dmitrić, Marko, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Vasković, Nikola, Matović, Kazimir, Karabasil, Nedjeljko, "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia" in Journal of Parasitology, 104, no. 5 (2018):557-559,
https://doi.org/10.1645/18-42 . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 

@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}

Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439

Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .

Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Sarić, Ljubiša
AU  - Arsić, Milos
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1641
AB  - The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.
PB  - Wiley, Hoboken
T2  - Journal of Food Processing and Preservation
T1  - In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food
VL  - 42
IS  - 2
SP  - e13455
DO  - 10.1111/jfpp.13455
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Šekler, Milanko and Sarić, Ljubiša and Arsić, Milos and Karabasil, Nedjeljko",
year = "2018",
abstract = "The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Processing and Preservation",
title = "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food",
volume = "42",
number = "2",
pages = "e13455",
doi = "10.1111/jfpp.13455"
}

Dmitrić, M., Vidanović, D., Matović, K., Šekler, M., Sarić, L., Arsić, M.,& Karabasil, N.. (2018). In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation
Wiley, Hoboken., 42(2), e13455.
https://doi.org/10.1111/jfpp.13455

Dmitrić M, Vidanović D, Matović K, Šekler M, Sarić L, Arsić M, Karabasil N. In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation. 2018;42(2):e13455.
doi:10.1111/jfpp.13455 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Šekler, Milanko, Sarić, Ljubiša, Arsić, Milos, Karabasil, Nedjeljko, "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food" in Journal of Food Processing and Preservation, 42, no. 2 (2018):e13455,
https://doi.org/10.1111/jfpp.13455 . .

TY  - JOUR
AU  - Kureljušić, Jasna
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Teodorović, Vlado
AU  - Kureljušić, Branislav
AU  - Velhner, Maja
AU  - Karabasil, Nedjeljko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1465
AB  - Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance
VL  - 11
IS  - 8
SP  - 640
EP  - 645
DO  - 10.3855/jidc.9311
ER  - 

@article{
author = "Kureljušić, Jasna and Dmitrić, Marko and Vidanović, Dejan and Teodorović, Vlado and Kureljušić, Branislav and Velhner, Maja and Karabasil, Nedjeljko",
year = "2017",
abstract = "Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance",
volume = "11",
number = "8",
pages = "640-645",
doi = "10.3855/jidc.9311"
}

Kureljušić, J., Dmitrić, M., Vidanović, D., Teodorović, V., Kureljušić, B., Velhner, M.,& Karabasil, N.. (2017). Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 11(8), 640-645.
https://doi.org/10.3855/jidc.9311

Kureljušić J, Dmitrić M, Vidanović D, Teodorović V, Kureljušić B, Velhner M, Karabasil N. Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance. in Journal of Infection in Developing Countries. 2017;11(8):640-645.
doi:10.3855/jidc.9311 .

Kureljušić, Jasna, Dmitrić, Marko, Vidanović, Dejan, Teodorović, Vlado, Kureljušić, Branislav, Velhner, Maja, Karabasil, Nedjeljko, "Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance" in Journal of Infection in Developing Countries, 11, no. 8 (2017):640-645,
https://doi.org/10.3855/jidc.9311 . .

TY  - CONF
AU  - Rašeta, Mladen
AU  - Mrdović, Boris
AU  - Janković, V.
AU  - Becskei, Zsolt
AU  - Lakićević, Brankica
AU  - Vidanović, Dejan
AU  - Polaček, Vladimir
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1551
AB  - This study aimed to determine Salmonella spp. prevalence in meat products, meat preparations and minced meat. Over a period of three years, a total of 300 samples were taken (100 RTE meat products, 100 meat preparations and 100 minced meat) and examined for the presence of Salmonella spp. Sampling was carried out at the warehouses of the food manufacturers. Salmonella spp. were not detected in RTE meat products, while 7% of semi-finished meat products (fresh sausages, grill meat formed and unformed) contained Salmonella, as did 18% of minced meats (minced pork II category, minced beef II category, mixed minced meat). The 25 Salmonella isolates obtained were examined for antibiotic resistance by the disk diffusion test, according to the NCCLS and CLSI guidelines. Isolates showed resistance to ampicillin and nalidixic acid (80%), tetracycline (72%), cefotaxime/clavulanic acid (48%), but not to gentamicin (8%) or trimethoprim/sulfamethoxazole (0%).
PB  - Iop Publishing Ltd, Bristol
C3  - 59th International Meat Industry Conference (MEATCON2017)
T1  - Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat
VL  - 85
SP  - UNSP 012028
DO  - 10.1088/1755-1315/85/1/012028
ER  - 

@conference{
author = "Rašeta, Mladen and Mrdović, Boris and Janković, V. and Becskei, Zsolt and Lakićević, Brankica and Vidanović, Dejan and Polaček, Vladimir",
year = "2017",
abstract = "This study aimed to determine Salmonella spp. prevalence in meat products, meat preparations and minced meat. Over a period of three years, a total of 300 samples were taken (100 RTE meat products, 100 meat preparations and 100 minced meat) and examined for the presence of Salmonella spp. Sampling was carried out at the warehouses of the food manufacturers. Salmonella spp. were not detected in RTE meat products, while 7% of semi-finished meat products (fresh sausages, grill meat formed and unformed) contained Salmonella, as did 18% of minced meats (minced pork II category, minced beef II category, mixed minced meat). The 25 Salmonella isolates obtained were examined for antibiotic resistance by the disk diffusion test, according to the NCCLS and CLSI guidelines. Isolates showed resistance to ampicillin and nalidixic acid (80%), tetracycline (72%), cefotaxime/clavulanic acid (48%), but not to gentamicin (8%) or trimethoprim/sulfamethoxazole (0%).",
publisher = "Iop Publishing Ltd, Bristol",
journal = "59th International Meat Industry Conference (MEATCON2017)",
title = "Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat",
volume = "85",
pages = "UNSP 012028",
doi = "10.1088/1755-1315/85/1/012028"
}

Rašeta, M., Mrdović, B., Janković, V., Becskei, Z., Lakićević, B., Vidanović, D.,& Polaček, V.. (2017). Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat. in 59th International Meat Industry Conference (MEATCON2017)
Iop Publishing Ltd, Bristol., 85, UNSP 012028.
https://doi.org/10.1088/1755-1315/85/1/012028

Rašeta M, Mrdović B, Janković V, Becskei Z, Lakićević B, Vidanović D, Polaček V. Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat. in 59th International Meat Industry Conference (MEATCON2017). 2017;85:UNSP 012028.
doi:10.1088/1755-1315/85/1/012028 .

Rašeta, Mladen, Mrdović, Boris, Janković, V., Becskei, Zsolt, Lakićević, Brankica, Vidanović, Dejan, Polaček, Vladimir, "Prevalence and antibiotic resistance of Salmonella spp. in meat products, meat preparations and minced meat" in 59th International Meat Industry Conference (MEATCON2017), 85 (2017):UNSP 012028,
https://doi.org/10.1088/1755-1315/85/1/012028 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Debeljak, Zoran
AU  - Karabasil, Nedjeljko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1483
AB  - Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.
PB  - Amer Assoc Zoo Veterinarians, Yulee
T2  - Journal of Zoo and Wildlife Medicine
T1  - Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia
VL  - 48
IS  - 3
SP  - 703
EP  - 707
DO  - 10.1638/2016-0169.1
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Vasković, Nikola and Matović, Kazimir and Šekler, Milanko and Debeljak, Zoran and Karabasil, Nedjeljko",
year = "2017",
abstract = "Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.",
publisher = "Amer Assoc Zoo Veterinarians, Yulee",
journal = "Journal of Zoo and Wildlife Medicine",
title = "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia",
volume = "48",
number = "3",
pages = "703-707",
doi = "10.1638/2016-0169.1"
}

Dmitrić, M., Vidanović, D., Vasković, N., Matović, K., Šekler, M., Debeljak, Z.,& Karabasil, N.. (2017). Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine
Amer Assoc Zoo Veterinarians, Yulee., 48(3), 703-707.
https://doi.org/10.1638/2016-0169.1

Dmitrić M, Vidanović D, Vasković N, Matović K, Šekler M, Debeljak Z, Karabasil N. Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine. 2017;48(3):703-707.
doi:10.1638/2016-0169.1 .

Dmitrić, Marko, Vidanović, Dejan, Vasković, Nikola, Matović, Kazimir, Šekler, Milanko, Debeljak, Zoran, Karabasil, Nedjeljko, "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia" in Journal of Zoo and Wildlife Medicine, 48, no. 3 (2017):703-707,
https://doi.org/10.1638/2016-0169.1 . .

TY  - JOUR
AU  - Debeljak, Zoran
AU  - Boufana, Belgees
AU  - Interisano, Maria
AU  - Vidanović, Dejan
AU  - Kulišić, Zoran
AU  - Casulli, Adriano
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1404
AB  - Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia
VL  - 223
SP  - 57
EP  - 62
DO  - 10.1016/j.vetpar.2016.04.007
ER  - 

@article{
author = "Debeljak, Zoran and Boufana, Belgees and Interisano, Maria and Vidanović, Dejan and Kulišić, Zoran and Casulli, Adriano",
year = "2016",
abstract = "Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia",
volume = "223",
pages = "57-62",
doi = "10.1016/j.vetpar.2016.04.007"
}

Debeljak, Z., Boufana, B., Interisano, M., Vidanović, D., Kulišić, Z.,& Casulli, A.. (2016). First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 223, 57-62.
https://doi.org/10.1016/j.vetpar.2016.04.007

Debeljak Z, Boufana B, Interisano M, Vidanović D, Kulišić Z, Casulli A. First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia. in Veterinary Parasitology. 2016;223:57-62.
doi:10.1016/j.vetpar.2016.04.007 .

Debeljak, Zoran, Boufana, Belgees, Interisano, Maria, Vidanović, Dejan, Kulišić, Zoran, Casulli, Adriano, "First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia" in Veterinary Parasitology, 223 (2016):57-62,
https://doi.org/10.1016/j.vetpar.2016.04.007 . .

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Vidanović, Dejan
AU  - Suvajdzić, Ljiljana
AU  - Stojanov, Igor
AU  - Krnjaić, Dejan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1191
AB  - Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia
VL  - 65
IS  - 4
SP  - 548
EP  - 556
DO  - 10.1515/acve-2015-0046
ER  - 

@article{
author = "Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Vidanović, Dejan and Suvajdzić, Ljiljana and Stojanov, Igor and Krnjaić, Dejan",
year = "2015",
abstract = "Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia",
volume = "65",
number = "4",
pages = "548-556",
doi = "10.1515/acve-2015-0046"
}

Todorović, D., Velhner, M., Milanov, D., Vidanović, D., Suvajdzić, L., Stojanov, I.,& Krnjaić, D.. (2015). Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 548-556.
https://doi.org/10.1515/acve-2015-0046

Todorović D, Velhner M, Milanov D, Vidanović D, Suvajdzić L, Stojanov I, Krnjaić D. Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):548-556.
doi:10.1515/acve-2015-0046 .

Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Vidanović, Dejan, Suvajdzić, Ljiljana, Stojanov, Igor, Krnjaić, Dejan, "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):548-556,
https://doi.org/10.1515/acve-2015-0046 . .

TY  - JOUR
AU  - Rašeta, Mladen
AU  - Teodorović, Vlado
AU  - Jovanović, Jelena
AU  - Lakićević, Brankica
AU  - Branković-Lazić, Ivana
AU  - Vidanović, Dejan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1260
AB  - During the validation and verification of HACCP system, a food business operator must use the data obtained in the regular control, whose dynamics and extent are required by actual legislation. During one year, at a cattle slaughterhouse in North Banat District (Serbia), swabs were continually taken from the carcasses of pigs, on the slaughter line after the final wash, in order to monitor compliance with the process hygiene criteria (Salmonella spp., Enterobacteriaceae, total viable count of aerobic bacteria). For 30 consecutive weeks of testing, , Salmonella spp. presence was not detected on the pig carcasses after final wash, while the determined presence of Enterobacteriaceae were 1,05 ± 0,78 log CFU/cm2, and the total viable count of aerobic bacteria were 2.87 ± 0.96 log CFU/cm2. The analysis of the obtained results proved a downward trend in Enterobacteriacae and total viable count of aerobic bacteria presence which proves the effectiveness of the new approach in the monitoring process hygiene during the production of pig carcasses, in accordance with the actual legislation.
AB  - Tokom validacije i verifikacije HACCP sistema, subjekt u poslovanju hranom, treba da upotrebi podatke dobijene u redovnoj kontroli čija su dinamika i obim propisani odgovarajućom zakonskom regulativom. U periodu od godinu dana na jednoj klanici u Severnobanatskom okrugu, vršeno je plansko uzimanje briseva sa trupova svinja sa ciljem praćenja higijene procesa klanja. Ispitivanjima su bili obuhvaćeni sledeći mikroorganizmi: Enterobacteriaceae i broj aerobnih bakterija, kao i potencijalno prisustvo patogena (Salmonella spp.). Tokom 30 uzastopnih nedelja ispitivanja, na trupovima svinja nakon završnog pranja, nije utvrđeno prisustvo Salmonella spp., dok je prisustvo Enterobacteriaceae iznosilo 1,05 ± 0,78 log CFU/cm2, a ukupnog broja aerobnih bakterija 2,87 ± 0,96 log CFU/cm2. Analizom dobijenih rezultata primetan je opadajući trend nalaza Enterobacteriacae i broja aerobnih bakterija, što ukazuje na efektivnost sistema HACCP i kontrole higijene procesa klanja koja je u skladu sa aktuelnom zakonskom regulativom.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Process hygiene of pig carcasses during one year at a slaughterhouse in the North Banat District of Serbia
T1  - Higijena procesa klanja i obrade svinja tokom godinu dana na jednoj klanici u Severnobanatskom okrugu u Srbiji
VL  - 56
IS  - 1
SP  - 26
EP  - 33
DO  - 10.5937/tehmesa1501026R
ER  - 

@article{
author = "Rašeta, Mladen and Teodorović, Vlado and Jovanović, Jelena and Lakićević, Brankica and Branković-Lazić, Ivana and Vidanović, Dejan",
year = "2015",
abstract = "During the validation and verification of HACCP system, a food business operator must use the data obtained in the regular control, whose dynamics and extent are required by actual legislation. During one year, at a cattle slaughterhouse in North Banat District (Serbia), swabs were continually taken from the carcasses of pigs, on the slaughter line after the final wash, in order to monitor compliance with the process hygiene criteria (Salmonella spp., Enterobacteriaceae, total viable count of aerobic bacteria). For 30 consecutive weeks of testing, , Salmonella spp. presence was not detected on the pig carcasses after final wash, while the determined presence of Enterobacteriaceae were 1,05 ± 0,78 log CFU/cm2, and the total viable count of aerobic bacteria were 2.87 ± 0.96 log CFU/cm2. The analysis of the obtained results proved a downward trend in Enterobacteriacae and total viable count of aerobic bacteria presence which proves the effectiveness of the new approach in the monitoring process hygiene during the production of pig carcasses, in accordance with the actual legislation., Tokom validacije i verifikacije HACCP sistema, subjekt u poslovanju hranom, treba da upotrebi podatke dobijene u redovnoj kontroli čija su dinamika i obim propisani odgovarajućom zakonskom regulativom. U periodu od godinu dana na jednoj klanici u Severnobanatskom okrugu, vršeno je plansko uzimanje briseva sa trupova svinja sa ciljem praćenja higijene procesa klanja. Ispitivanjima su bili obuhvaćeni sledeći mikroorganizmi: Enterobacteriaceae i broj aerobnih bakterija, kao i potencijalno prisustvo patogena (Salmonella spp.). Tokom 30 uzastopnih nedelja ispitivanja, na trupovima svinja nakon završnog pranja, nije utvrđeno prisustvo Salmonella spp., dok je prisustvo Enterobacteriaceae iznosilo 1,05 ± 0,78 log CFU/cm2, a ukupnog broja aerobnih bakterija 2,87 ± 0,96 log CFU/cm2. Analizom dobijenih rezultata primetan je opadajući trend nalaza Enterobacteriacae i broja aerobnih bakterija, što ukazuje na efektivnost sistema HACCP i kontrole higijene procesa klanja koja je u skladu sa aktuelnom zakonskom regulativom.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Process hygiene of pig carcasses during one year at a slaughterhouse in the North Banat District of Serbia, Higijena procesa klanja i obrade svinja tokom godinu dana na jednoj klanici u Severnobanatskom okrugu u Srbiji",
volume = "56",
number = "1",
pages = "26-33",
doi = "10.5937/tehmesa1501026R"
}

Rašeta, M., Teodorović, V., Jovanović, J., Lakićević, B., Branković-Lazić, I.,& Vidanović, D.. (2015). Process hygiene of pig carcasses during one year at a slaughterhouse in the North Banat District of Serbia. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 56(1), 26-33.
https://doi.org/10.5937/tehmesa1501026R

Rašeta M, Teodorović V, Jovanović J, Lakićević B, Branković-Lazić I, Vidanović D. Process hygiene of pig carcasses during one year at a slaughterhouse in the North Banat District of Serbia. in Tehnologija mesa. 2015;56(1):26-33.
doi:10.5937/tehmesa1501026R .

Rašeta, Mladen, Teodorović, Vlado, Jovanović, Jelena, Lakićević, Brankica, Branković-Lazić, Ivana, Vidanović, Dejan, "Process hygiene of pig carcasses during one year at a slaughterhouse in the North Banat District of Serbia" in Tehnologija mesa, 56, no. 1 (2015):26-33,
https://doi.org/10.5937/tehmesa1501026R . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Petrić, Dušan
AU  - Vasić, Ana
AU  - Hrnjaković-Cvjetković, Ivana
AU  - Milošević, Vesna
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Lazić, Sava
AU  - Đuričić, Bosiljka
AU  - Plavšić, Budimir
AU  - Juan-Carlos, Saiz
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1205
AB  - West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed.
AB  - Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - WNV infection: An emergent vector borne viral infection in Serbia: Current situation
T1  - Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija
VL  - 69
IS  - 1-2
SP  - 111
EP  - 126
DO  - 10.2298/VETGL1502111P
ER  - 

@article{
author = "Petrović, Tamaš and Lupulović, Diana and Petrić, Dušan and Vasić, Ana and Hrnjaković-Cvjetković, Ivana and Milošević, Vesna and Vidanović, Dejan and Šekler, Milanko and Lazić, Sava and Đuričić, Bosiljka and Plavšić, Budimir and Juan-Carlos, Saiz",
year = "2015",
abstract = "West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed., Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "WNV infection: An emergent vector borne viral infection in Serbia: Current situation, Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija",
volume = "69",
number = "1-2",
pages = "111-126",
doi = "10.2298/VETGL1502111P"
}

Petrović, T., Lupulović, D., Petrić, D., Vasić, A., Hrnjaković-Cvjetković, I., Milošević, V., Vidanović, D., Šekler, M., Lazić, S., Đuričić, B., Plavšić, B.,& Juan-Carlos, S.. (2015). WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 111-126.
https://doi.org/10.2298/VETGL1502111P

Petrović T, Lupulović D, Petrić D, Vasić A, Hrnjaković-Cvjetković I, Milošević V, Vidanović D, Šekler M, Lazić S, Đuričić B, Plavšić B, Juan-Carlos S. WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik. 2015;69(1-2):111-126.
doi:10.2298/VETGL1502111P .

Petrović, Tamaš, Lupulović, Diana, Petrić, Dušan, Vasić, Ana, Hrnjaković-Cvjetković, Ivana, Milošević, Vesna, Vidanović, Dejan, Šekler, Milanko, Lazić, Sava, Đuričić, Bosiljka, Plavšić, Budimir, Juan-Carlos, Saiz, "WNV infection: An emergent vector borne viral infection in Serbia: Current situation" in Veterinarski Glasnik, 69, no. 1-2 (2015):111-126,
https://doi.org/10.2298/VETGL1502111P . .