TY  - JOUR
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Maletić, Milan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2815
AB  - Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: Adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity. In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (≥ 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera. The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control
PB  - Sciendo
T2  - Acta Veterinaria
T1  - Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination
VL  - 73
IS  - 1
SP  - 133
EP  - 142
DO  - 10.2478/acve-2023-0010
ER  - 

@article{
author = "Maletić, Jelena and Spalević, Ljiljana and Kureljušić, Branislav and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Maletić, Milan and Milićević, Vesna",
year = "2023",
abstract = "Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: Adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity. In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (≥ 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera. The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control",
publisher = "Sciendo",
journal = "Acta Veterinaria",
title = "Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination",
volume = "73",
number = "1",
pages = "133-142",
doi = "10.2478/acve-2023-0010"
}

Maletić, J., Spalević, L., Kureljušić, B., Veljović, L., Maksimović-Zorić, J., Maletić, M.,& Milićević, V.. (2023). Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination. in Acta Veterinaria
Sciendo., 73(1), 133-142.
https://doi.org/10.2478/acve-2023-0010

Maletić J, Spalević L, Kureljušić B, Veljović L, Maksimović-Zorić J, Maletić M, Milićević V. Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination. in Acta Veterinaria. 2023;73(1):133-142.
doi:10.2478/acve-2023-0010 .

Maletić, Jelena, Spalević, Ljiljana, Kureljušić, Branislav, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Maletić, Milan, Milićević, Vesna, "Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination" in Acta Veterinaria, 73, no. 1 (2023):133-142,
https://doi.org/10.2478/acve-2023-0010 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Toplak, Ivan
AU  - Prodanović, Radiša
AU  - Gallardo, Carmina
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2947
AB  - This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution
PB  - Springer
T2  - Veterinary Research Communications
T1  - Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia
VL  - 47
IS  - 4
SP  - 1925
EP  - 1936
DO  - 10.1007/s11259-023-10145-7
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Toplak, Ivan and Prodanović, Radiša and Gallardo, Carmina and Radojičić, Sonja",
year = "2023",
abstract = "This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia",
volume = "47",
number = "4",
pages = "1925-1936",
doi = "10.1007/s11259-023-10145-7"
}

Glišić, D., Milićević, V., Krnjaić, D., Toplak, I., Prodanović, R., Gallardo, C.,& Radojičić, S.. (2023). Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications
Springer., 47(4), 1925-1936.
https://doi.org/10.1007/s11259-023-10145-7

Glišić D, Milićević V, Krnjaić D, Toplak I, Prodanović R, Gallardo C, Radojičić S. Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications. 2023;47(4):1925-1936.
doi:10.1007/s11259-023-10145-7 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Toplak, Ivan, Prodanović, Radiša, Gallardo, Carmina, Radojičić, Sonja, "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia" in Veterinary Research Communications, 47, no. 4 (2023):1925-1936,
https://doi.org/10.1007/s11259-023-10145-7 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Ðordević, Igor
AU  - Anđelković, Katarina
AU  - Petković, Jelena
AU  - Dačić, Miroljub
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2540
AB  - The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022
PB  - MDPI
T2  - Pathogens
T1  - Patterns of ASFV Transmission in Domestic Pigs in Serbia
VL  - 12
IS  - 149
DO  - 10.3390/pathogens12010149
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Veljović, Ljubiša and Milovanović, Bojan and Kureljušić, Branislav and Ðordević, Igor and Anđelković, Katarina and Petković, Jelena and Dačić, Miroljub",
year = "2023",
abstract = "The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022",
publisher = "MDPI",
journal = "Pathogens",
title = "Patterns of ASFV Transmission in Domestic Pigs in Serbia",
volume = "12",
number = "149",
doi = "10.3390/pathogens12010149"
}

Glišić, D., Milićević, V., Veljović, L., Milovanović, B., Kureljušić, B., Ðordević, I., Anđelković, K., Petković, J.,& Dačić, M.. (2023). Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens
MDPI., 12(149).
https://doi.org/10.3390/pathogens12010149

Glišić D, Milićević V, Veljović L, Milovanović B, Kureljušić B, Ðordević I, Anđelković K, Petković J, Dačić M. Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens. 2023;12(149).
doi:10.3390/pathogens12010149 .

Glišić, Dimitrije, Milićević, Vesna, Veljović, Ljubiša, Milovanović, Bojan, Kureljušić, Branislav, Ðordević, Igor, Anđelković, Katarina, Petković, Jelena, Dačić, Miroljub, "Patterns of ASFV Transmission in Domestic Pigs in Serbia" in Pathogens, 12, no. 149 (2023),
https://doi.org/10.3390/pathogens12010149 . .

TY  - JOUR
AU  - Sapundžić Zurovac, Zorana
AU  - Žutić, Jadranka
AU  - Stević, Nataša
AU  - Milićević, Vesna
AU  - Radojičić, Marina
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2510
AB  - Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.
PB  - MDPI
T2  - Veterinary Sciences
T1  - First Report of Brucella Seroprevalence in Wild Boar Population in Serbia
VL  - 9
IS  - 10
SP  - 575
DO  - 10.3390/vetsci9100575
ER  - 

@article{
author = "Sapundžić Zurovac, Zorana and Žutić, Jadranka and Stević, Nataša and Milićević, Vesna and Radojičić, Marina and Stanojević, Slavoljub and Radojičić, Sonja",
year = "2022",
abstract = "Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.",
publisher = "MDPI",
journal = "Veterinary Sciences",
title = "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia",
volume = "9",
number = "10",
pages = "575",
doi = "10.3390/vetsci9100575"
}

Sapundžić Zurovac, Z., Žutić, J., Stević, N., Milićević, V., Radojičić, M., Stanojević, S.,& Radojičić, S.. (2022). First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences
MDPI., 9(10), 575.
https://doi.org/10.3390/vetsci9100575

Sapundžić Zurovac Z, Žutić J, Stević N, Milićević V, Radojičić M, Stanojević S, Radojičić S. First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences. 2022;9(10):575.
doi:10.3390/vetsci9100575 .

Sapundžić Zurovac, Zorana, Žutić, Jadranka, Stević, Nataša, Milićević, Vesna, Radojičić, Marina, Stanojević, Slavoljub, Radojičić, Sonja, "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia" in Veterinary Sciences, 9, no. 10 (2022):575,
https://doi.org/10.3390/vetsci9100575 . .

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Spalević, Ljiljana
AU  - Vučićević, Ivana
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2866
AB  - Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many
reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian
leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders
in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia,
apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching
20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis,
splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart.
Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while
granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and
arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces
revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of
oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis
virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as
molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus.
AB  - Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća
opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i
virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski
gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane
simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće
promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju,
višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene,
proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin
srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim
granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog
crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove
bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i
molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.
C3  - XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022
T1  - Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens
T1  - Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan
SP  - 210
EP  - 217
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2866
ER  - 

@conference{
author = "Maletić, Jelena and Kureljušić, Branislav and Spalević, Ljiljana and Vučićević, Ivana and Veljović, Ljubiša and Milovanović, Bojan and Milićević, Vesna",
year = "2022",
abstract = "Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many
reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian
leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders
in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia,
apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching
20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis,
splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart.
Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while
granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and
arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces
revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of
oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis
virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as
molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus., Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća
opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i
virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski
gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane
simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće
promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju,
višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene,
proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin
srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim
granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog
crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove
bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i
molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.",
journal = "XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022",
title = "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens, Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan",
pages = "210-217",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2866"
}

Maletić, J., Kureljušić, B., Spalević, L., Vučićević, I., Veljović, L., Milovanović, B.,& Milićević, V.. (2022). Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022, 210-217.
https://hdl.handle.net/21.15107/rcub_veterinar_2866

Maletić J, Kureljušić B, Spalević L, Vučićević I, Veljović L, Milovanović B, Milićević V. Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022. 2022;:210-217.
https://hdl.handle.net/21.15107/rcub_veterinar_2866 .

Maletić, Jelena, Kureljušić, Branislav, Spalević, Ljiljana, Vučićević, Ivana, Veljović, Ljubiša, Milovanović, Bojan, Milićević, Vesna, "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens" in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022 (2022):210-217,
https://hdl.handle.net/21.15107/rcub_veterinar_2866 .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Ćirović, Duško
AU  - Kuručki, Milica
AU  - Glišić, Dimitrije
AU  - Sapundžić Zurovac, Zorana
AU  - Milovanović, Bojan
AU  - Weissenbacher-Lang, Christiane
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2545
AB  - Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.
PB  - Wildlife Disease Association
T2  - Journal of wildlife diseases
T1  - First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)
VL  - 58
IS  - 4
SP  - 897
EP  - 901
DO  - 10.7589/JWD-D-22-00007
ER  - 

@article{
author = "Kureljušić, Branislav and Milićević, Vesna and Ćirović, Duško and Kuručki, Milica and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Milovanović, Bojan and Weissenbacher-Lang, Christiane",
year = "2022",
abstract = "Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.",
publisher = "Wildlife Disease Association",
journal = "Journal of wildlife diseases",
title = "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)",
volume = "58",
number = "4",
pages = "897-901",
doi = "10.7589/JWD-D-22-00007"
}

Kureljušić, B., Milićević, V., Ćirović, D., Kuručki, M., Glišić, D., Sapundžić Zurovac, Z., Milovanović, B.,& Weissenbacher-Lang, C.. (2022). First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases
Wildlife Disease Association., 58(4), 897-901.
https://doi.org/10.7589/JWD-D-22-00007

Kureljušić B, Milićević V, Ćirović D, Kuručki M, Glišić D, Sapundžić Zurovac Z, Milovanović B, Weissenbacher-Lang C. First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases. 2022;58(4):897-901.
doi:10.7589/JWD-D-22-00007 .

Kureljušić, Branislav, Milićević, Vesna, Ćirović, Duško, Kuručki, Milica, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Milovanović, Bojan, Weissenbacher-Lang, Christiane, "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)" in Journal of wildlife diseases, 58, no. 4 (2022):897-901,
https://doi.org/10.7589/JWD-D-22-00007 . .

TY  - JOUR
AU  - Radojičić, Sonja
AU  - Živulj, Aleksandar
AU  - Petrovic, Tamas
AU  - Nišavić, Jakov
AU  - Milićević, Vesna
AU  - Sipetic-Grujicic, Sandra
AU  - Mišić, Dusan
AU  - Korzeniowska, Malgorzata
AU  - Stanojević, Slavoljub
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2298
AB  - West Nile virus (WNV) is an arthropod-born pathogen, which is transmitted from wild birds through mosquitoes to humans and animals. At the end of the 20th century, the first West Nile fever (WNF) outbreaks among humans in urban environments in Eastern Europe and the United States were reported. The disease continued to spread to other parts of the continents. In Serbia, the largest number of WNV-infected people was recorded in 2018. This research used spatial statistics to identify clusters of WNV infection in humans and animals in South Banat County, Serbia. The occurrence of WNV infection and risk factors were analyzed using a negative binomial regression model. Our research indicated that climatic factors were the main determinant of WNV distribution and were predictors of endemicity. Precipitation and water levels of rivers had an important influence on mosquito abundance and affected the habitats of wild birds, which are important for maintaining the virus in nature. We found that the maximum temperature of the warmest part of the year and the annual temperature range; and hydrographic variables, e.g., the presence of rivers and water streams were the best environmental predictors of WNF outbreaks in South Banat County.
PB  - MDPI
T2  - Animals
T1  - Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019
VL  - 11
IS  - 10
SP  - 2951
DO  - 10.3390/ani11102951
ER  - 

@article{
author = "Radojičić, Sonja and Živulj, Aleksandar and Petrovic, Tamas and Nišavić, Jakov and Milićević, Vesna and Sipetic-Grujicic, Sandra and Mišić, Dusan and Korzeniowska, Malgorzata and Stanojević, Slavoljub",
year = "2021",
abstract = "West Nile virus (WNV) is an arthropod-born pathogen, which is transmitted from wild birds through mosquitoes to humans and animals. At the end of the 20th century, the first West Nile fever (WNF) outbreaks among humans in urban environments in Eastern Europe and the United States were reported. The disease continued to spread to other parts of the continents. In Serbia, the largest number of WNV-infected people was recorded in 2018. This research used spatial statistics to identify clusters of WNV infection in humans and animals in South Banat County, Serbia. The occurrence of WNV infection and risk factors were analyzed using a negative binomial regression model. Our research indicated that climatic factors were the main determinant of WNV distribution and were predictors of endemicity. Precipitation and water levels of rivers had an important influence on mosquito abundance and affected the habitats of wild birds, which are important for maintaining the virus in nature. We found that the maximum temperature of the warmest part of the year and the annual temperature range; and hydrographic variables, e.g., the presence of rivers and water streams were the best environmental predictors of WNF outbreaks in South Banat County.",
publisher = "MDPI",
journal = "Animals",
title = "Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019",
volume = "11",
number = "10",
pages = "2951",
doi = "10.3390/ani11102951"
}

Radojičić, S., Živulj, A., Petrovic, T., Nišavić, J., Milićević, V., Sipetic-Grujicic, S., Mišić, D., Korzeniowska, M.,& Stanojević, S.. (2021). Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019. in Animals
MDPI., 11(10), 2951.
https://doi.org/10.3390/ani11102951

Radojičić S, Živulj A, Petrovic T, Nišavić J, Milićević V, Sipetic-Grujicic S, Mišić D, Korzeniowska M, Stanojević S. Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019. in Animals. 2021;11(10):2951.
doi:10.3390/ani11102951 .

Radojičić, Sonja, Živulj, Aleksandar, Petrovic, Tamas, Nišavić, Jakov, Milićević, Vesna, Sipetic-Grujicic, Sandra, Mišić, Dusan, Korzeniowska, Malgorzata, Stanojević, Slavoljub, "Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019" in Animals, 11, no. 10 (2021):2951,
https://doi.org/10.3390/ani11102951 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Aleksić-Kovačević, Sanja
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
AU  - Maksimović Zorić, Jelena
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Knežević, Đorđe
AU  - Spalević, Ljiljana
AU  - Kukolj, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2784
AB  - Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.
PB  - Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)
T2  - Veterinaria Italiana
T1  - Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
VL  - 54
IS  - 7
SP  - 287
EP  - 295
DO  - 10.12834/VetIt.1813.9553.3
ER  - 

@article{
author = "Kureljušić, Branislav and Aleksić-Kovačević, Sanja and Savić, Božidar and Prodanović, Radiša and Jezdimirović, Nemanja and Milićević, Vesna and Maksimović Zorić, Jelena and Kureljušić, Jasna and Žutić, Jadranka and Knežević, Đorđe and Spalević, Ljiljana and Kukolj, Vladimir",
year = "2021",
abstract = "Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.",
publisher = "Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)",
journal = "Veterinaria Italiana",
title = "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus",
volume = "54",
number = "7",
pages = "287-295",
doi = "10.12834/VetIt.1813.9553.3"
}

Kureljušić, B., Aleksić-Kovačević, S., Savić, B., Prodanović, R., Jezdimirović, N., Milićević, V., Maksimović Zorić, J., Kureljušić, J., Žutić, J., Knežević, Đ., Spalević, L.,& Kukolj, V.. (2021). Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)., 54(7), 287-295.
https://doi.org/10.12834/VetIt.1813.9553.3

Kureljušić B, Aleksić-Kovačević S, Savić B, Prodanović R, Jezdimirović N, Milićević V, Maksimović Zorić J, Kureljušić J, Žutić J, Knežević Đ, Spalević L, Kukolj V. Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana. 2021;54(7):287-295.
doi:10.12834/VetIt.1813.9553.3 .

Kureljušić, Branislav, Aleksić-Kovačević, Sanja, Savić, Božidar, Prodanović, Radiša, Jezdimirović, Nemanja, Milićević, Vesna, Maksimović Zorić, Jelena, Kureljušić, Jasna, Žutić, Jadranka, Knežević, Đorđe, Spalević, Ljiljana, Kukolj, Vladimir, "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus" in Veterinaria Italiana, 54, no. 7 (2021):287-295,
https://doi.org/10.12834/VetIt.1813.9553.3 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Savić, Božidar
AU  - Radojičić, Sonja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1793
AB  - Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The performance of seven molecular methods for the detection of prrsv
VL  - 70
IS  - 1
SP  - 51
EP  - 57
DO  - 10.2478/acve-2020-0003
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Veljović, Ljubiša and Valčić, Miroslav and Stević, Nataša and Savić, Božidar and Radojičić, Sonja",
year = "2020",
abstract = "Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The performance of seven molecular methods for the detection of prrsv",
volume = "70",
number = "1",
pages = "51-57",
doi = "10.2478/acve-2020-0003"
}

Milićević, V., Kureljušić, B., Veljović, L., Valčić, M., Stević, N., Savić, B.,& Radojičić, S.. (2020). The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 51-57.
https://doi.org/10.2478/acve-2020-0003

Milićević V, Kureljušić B, Veljović L, Valčić M, Stević N, Savić B, Radojičić S. The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd. 2020;70(1):51-57.
doi:10.2478/acve-2020-0003 .

Milićević, Vesna, Kureljušić, Branislav, Veljović, Ljubiša, Valčić, Miroslav, Stević, Nataša, Savić, Božidar, Radojičić, Sonja, "The performance of seven molecular methods for the detection of prrsv" in Acta Veterinaria-Beograd, 70, no. 1 (2020):51-57,
https://doi.org/10.2478/acve-2020-0003 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Hoffmann, Bernd
AU  - Dietze, Klaas
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1795
AB  - Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.
PB  - BMC, London
T2  - Virology Journal
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA
VL  - 17
IS  - 1
SP  - 28
DO  - 10.1186/s12985-020-01298-x
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Hoffmann, Bernd and Dietze, Klaas",
year = "2020",
abstract = "Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.",
publisher = "BMC, London",
journal = "Virology Journal",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA",
volume = "17",
number = "1",
pages = "28",
doi = "10.1186/s12985-020-01298-x"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Hoffmann, B.,& Dietze, K.. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal
BMC, London., 17(1), 28.
https://doi.org/10.1186/s12985-020-01298-x

Milovanović M, Milićević V, Radojičić S, Valčić M, Hoffmann B, Dietze K. Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal. 2020;17(1):28.
doi:10.1186/s12985-020-01298-x .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Hoffmann, Bernd, Dietze, Klaas, "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA" in Virology Journal, 17, no. 1 (2020):28,
https://doi.org/10.1186/s12985-020-01298-x . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1796
AB  - A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.
PB  - Polish Soc Veterinary Sciences Editorial Office, Lublin
T2  - Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.",
publisher = "Polish Soc Veterinary Sciences Editorial Office, Lublin",
journal = "Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
Polish Soc Veterinary Sciences Editorial Office, Lublin., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2017
AB  - A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.
PB  - Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)
T2  - Medycyna Weterynaryjna
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.",
publisher = "Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)",
journal = "Medycyna Weterynaryjna",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna
Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Stevančević, Ognjen
AU  - Kureljušić, Branislav
AU  - Nešić, Ksenija
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1990
AB  - Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.
PB  - Springer
T2  - Archives of Virology
T1  - Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia
VL  - 165
IS  - 1
SP  - 193
EP  - 199
DO  - 10.1007/s00705-019-04455-y
ER  - 

@article{
author = "Savić, Božidar and Milićević, Vesna and Radanović, Oliver and Zdravković, Nemanja and Stevančević, Ognjen and Kureljušić, Branislav and Nešić, Ksenija",
year = "2020",
abstract = "Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.",
publisher = "Springer",
journal = "Archives of Virology",
title = "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia",
volume = "165",
number = "1",
pages = "193-199",
doi = "10.1007/s00705-019-04455-y"
}

Savić, B., Milićević, V., Radanović, O., Zdravković, N., Stevančević, O., Kureljušić, B.,& Nešić, K.. (2020). Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology
Springer., 165(1), 193-199.
https://doi.org/10.1007/s00705-019-04455-y

Savić B, Milićević V, Radanović O, Zdravković N, Stevančević O, Kureljušić B, Nešić K. Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology. 2020;165(1):193-199.
doi:10.1007/s00705-019-04455-y .

Savić, Božidar, Milićević, Vesna, Radanović, Oliver, Zdravković, Nemanja, Stevančević, Ognjen, Kureljušić, Branislav, Nešić, Ksenija, "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia" in Archives of Virology, 165, no. 1 (2020):193-199,
https://doi.org/10.1007/s00705-019-04455-y . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Jasna
AU  - Milićević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Vesković Moračanin, Slavica
AU  - Žutić, Jadranka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1860
AB  - INTRODUCTION: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. METHODOLOGY: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. RESULTS: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). CONCLUSIONS: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.
PB  - NLM (Medline)
T2  - Journal of infection in developing countries
T1  - Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
VL  - 14
IS  - 6
SP  - 669
EP  - 673
DO  - 10.3855/jidc.12552
ER  - 

@article{
author = "Kureljušić, Branislav and Savić, Božidar and Jezdimirović, Nemanja and Kureljušić, Jasna and Milićević, Vesna and Karabasil, Nedjeljko and Vesković Moračanin, Slavica and Žutić, Jadranka",
year = "2020",
abstract = "INTRODUCTION: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. METHODOLOGY: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. RESULTS: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). CONCLUSIONS: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.",
publisher = "NLM (Medline)",
journal = "Journal of infection in developing countries",
title = "Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade",
volume = "14",
number = "6",
pages = "669-673",
doi = "10.3855/jidc.12552"
}

Kureljušić, B., Savić, B., Jezdimirović, N., Kureljušić, J., Milićević, V., Karabasil, N., Vesković Moračanin, S.,& Žutić, J.. (2020). Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade. in Journal of infection in developing countries
NLM (Medline)., 14(6), 669-673.
https://doi.org/10.3855/jidc.12552

Kureljušić B, Savić B, Jezdimirović N, Kureljušić J, Milićević V, Karabasil N, Vesković Moračanin S, Žutić J. Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade. in Journal of infection in developing countries. 2020;14(6):669-673.
doi:10.3855/jidc.12552 .

Kureljušić, Branislav, Savić, Božidar, Jezdimirović, Nemanja, Kureljušić, Jasna, Milićević, Vesna, Karabasil, Nedjeljko, Vesković Moračanin, Slavica, Žutić, Jadranka, "Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade" in Journal of infection in developing countries, 14, no. 6 (2020):669-673,
https://doi.org/10.3855/jidc.12552 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Maksimović-Zorić, Jelena
AU  - Savić, Božidar
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1985
AB  - Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia
VL  - 132
SP  - 404
EP  - 406
DO  - 10.1016/j.rvsc.2020.07.028
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Maksimović-Zorić, Jelena and Savić, Božidar and Spalević, Ljiljana and Žutić, Jadranka",
year = "2020",
abstract = "Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia",
volume = "132",
pages = "404-406",
doi = "10.1016/j.rvsc.2020.07.028"
}

Milićević, V., Kureljušić, B., Maksimović-Zorić, J., Savić, B., Spalević, L.,& Žutić, J.. (2020). Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science
Elsevier., 132, 404-406.
https://doi.org/10.1016/j.rvsc.2020.07.028

Milićević V, Kureljušić B, Maksimović-Zorić J, Savić B, Spalević L, Žutić J. Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science. 2020;132:404-406.
doi:10.1016/j.rvsc.2020.07.028 .

Milićević, Vesna, Kureljušić, Branislav, Maksimović-Zorić, Jelena, Savić, Božidar, Spalević, Ljiljana, Žutić, Jadranka, "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia" in Research in Veterinary Science, 132 (2020):404-406,
https://doi.org/10.1016/j.rvsc.2020.07.028 . .

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
AU  - Potkonjak, Aleksandar
AU  - Stojanac, Nenad
AU  - Kureljušić, Branislav
AU  - Veljović, Lubiša
AU  - Radosavljević, Vladimir
AU  - Savić, Božidar
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1987
AB  - Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage.
AB  - Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018
T1  - Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine
VL  - 70
IS  - 1
SP  - 110
EP  - 125
DO  - 10.2478/acve-2020-0008
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Stevančević, Ognjen and Chiapponi, Chiara and Potkonjak, Aleksandar and Stojanac, Nenad and Kureljušić, Branislav and Veljović, Lubiša and Radosavljević, Vladimir and Savić, Božidar",
year = "2020",
abstract = "Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage., Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.",
publisher = "Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018, Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine",
volume = "70",
number = "1",
pages = "110-125",
doi = "10.2478/acve-2020-0008"
}

Maksimović Zorić, J., Milićević, V., Stevančević, O., Chiapponi, C., Potkonjak, A., Stojanac, N., Kureljušić, B., Veljović, L., Radosavljević, V.,& Savić, B.. (2020). Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd
Sciendo., 70(1), 110-125.
https://doi.org/10.2478/acve-2020-0008

Maksimović Zorić J, Milićević V, Stevančević O, Chiapponi C, Potkonjak A, Stojanac N, Kureljušić B, Veljović L, Radosavljević V, Savić B. Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd. 2020;70(1):110-125.
doi:10.2478/acve-2020-0008 .

Maksimović Zorić, Jelena, Milićević, Vesna, Stevančević, Ognjen, Chiapponi, Chiara, Potkonjak, Aleksandar, Stojanac, Nenad, Kureljušić, Branislav, Veljović, Lubiša, Radosavljević, Vladimir, Savić, Božidar, "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018" in Acta Veterinaria-Beograd, 70, no. 1 (2020):110-125,
https://doi.org/10.2478/acve-2020-0008 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Nišavić, Jakov
AU  - Radojičić, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1781
AB  - Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia
VL  - 39
IS  - 3
SP  - 433
EP  - 437
DO  - 10.29261/pakvetj/2019.034
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Valčić, Miroslav and Stević, Nataša and Nišavić, Jakov and Radojičić, Sonja",
year = "2019",
abstract = "Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia",
volume = "39",
number = "3",
pages = "433-437",
doi = "10.29261/pakvetj/2019.034"
}

Milovanović, M., Milićević, V., Valčić, M., Stević, N., Nišavić, J.,& Radojičić, S.. (2019). Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 39(3), 433-437.
https://doi.org/10.29261/pakvetj/2019.034

Milovanović M, Milićević V, Valčić M, Stević N, Nišavić J, Radojičić S. Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal. 2019;39(3):433-437.
doi:10.29261/pakvetj/2019.034 .

Milovanović, Milovan, Milićević, Vesna, Valčić, Miroslav, Stević, Nataša, Nišavić, Jakov, Radojičić, Sonja, "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia" in Pakistan Veterinary Journal, 39, no. 3 (2019):433-437,
https://doi.org/10.29261/pakvetj/2019.034 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Dietze, Klaas
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Moritz, Tom
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1732
AB  - BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
VL  - 15
SP  - 80
DO  - 10.1186/s12917-019-1831-y
ER  - 

@article{
author = "Milovanović, Milovan and Dietze, Klaas and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Moritz, Tom and Hoffmann, Bernd",
year = "2019",
abstract = "BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests",
volume = "15",
pages = "80",
doi = "10.1186/s12917-019-1831-y"
}

Milovanović, M., Dietze, K., Milićević, V., Radojičić, S., Valčić, M., Moritz, T.,& Hoffmann, B.. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research
BMC, London., 15, 80.
https://doi.org/10.1186/s12917-019-1831-y

Milovanović M, Dietze K, Milićević V, Radojičić S, Valčić M, Moritz T, Hoffmann B. Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research. 2019;15:80.
doi:10.1186/s12917-019-1831-y .

Milovanović, Milovan, Dietze, Klaas, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Moritz, Tom, Hoffmann, Bernd, "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests" in BMC Veterinary Research, 15 (2019):80,
https://doi.org/10.1186/s12917-019-1831-y . .

TY  - CONF
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Dietze, Klaas
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2753
AB  - The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and
authorized for serum and plasma, recently improved the options for assessing the humoral immune
response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk)
and colostrum as samples for this ELISA was investigated.
Samples (serum, milk and colostrum) were collected three times (before, one and five months post
re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by
analyzing of 352 individual milk samples from Germany.
From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox
ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased
incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for
serum, whereas with modified protocol the number of positive sample increased up to 48. Only
two milk samples from Germany had border line results with the modified protocol. Sensitivity and
specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant
statistical difference (p<0.05) between both protocols. In addition, antibody detection was
possible in bulk milk and colostrum samples.
In principle, the commercially available Capripox ELISA, developed for antibody detection in serum
and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can
be a helpful tool in LSDV monitoring programs.
C3  - 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination
SP  - 204
EP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2753
ER  - 

@conference{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Dietze, Klaas and Hoffmann, Bernd",
year = "2019",
abstract = "The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and
authorized for serum and plasma, recently improved the options for assessing the humoral immune
response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk)
and colostrum as samples for this ELISA was investigated.
Samples (serum, milk and colostrum) were collected three times (before, one and five months post
re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by
analyzing of 352 individual milk samples from Germany.
From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox
ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased
incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for
serum, whereas with modified protocol the number of positive sample increased up to 48. Only
two milk samples from Germany had border line results with the modified protocol. Sensitivity and
specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant
statistical difference (p<0.05) between both protocols. In addition, antibody detection was
possible in bulk milk and colostrum samples.
In principle, the commercially available Capripox ELISA, developed for antibody detection in serum
and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can
be a helpful tool in LSDV monitoring programs.",
journal = "13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination",
pages = "204-204",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2753"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Dietze, K.,& Hoffmann, B.. (2019). Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019, 204-204.
https://hdl.handle.net/21.15107/rcub_veterinar_2753

Milovanović M, Milićević V, Radojičić S, Valčić M, Dietze K, Hoffmann B. Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019. 2019;:204-204.
https://hdl.handle.net/21.15107/rcub_veterinar_2753 .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Dietze, Klaas, Hoffmann, Bernd, "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination" in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019 (2019):204-204,
https://hdl.handle.net/21.15107/rcub_veterinar_2753 .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Radanović, Oliver
AU  - Milićević, Vesna
AU  - Vujanac, Ivan
AU  - Bojkovski, Jovan
AU  - Kureljušić, Branislav
AU  - Arsić, Sveta
AU  - Jovanović, Ljubomir
AU  - Kirovski, Danijela
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1705
AB  - Introduction. Neonatal calves are often deficient in iron. Accumulating evidence indicates that iron status is associated with disease pathologies including diarrhea. Our objective was to examine the association between iron status and gut function in neonatal calves with and without a history of calf diarrhea. Materials and Methods. Calves were divided into two groups based on their history of diarrhea; the first group were diarrheic calves (n=6) and the second group were non-diarrheic healthy calves (n=6). Blood samples (n=12) were collected at day 12 of age and erythrogram determination and measurements of serum iron and total iron binding capacity were performed. Hematological values were measured using an automatic analyzer, and biochemical properties were determined spectrophotometrically. Fecal samples were obtained from all calves and pH measured using semi quantitative test strips as well as being examined by bacterial cultivation for enterotoxigenic Escherichia coli, Salmonella spp. and Clostridium perfringens, by RT-PCR for the presence of bovine rotavirus, bovine coronavirus and bovine viral diarrhea virus, and by microscopy for the presence of Cryptosporidium parvum. Results and Conclusions. There were significant iron-related changes for most hematological indices in diarrheic calves; and iron (Fe) deficiency and microcytic, hypochromic anemia were diagnosed. The pH of the feces was significantly higher in diarrheic calves than in the non-diarrheic healthy group (P lt 0.01). All fecal samples were negative for the analyzed enteric pathogens. According to the results obtained, calves experiencing iron deficiency anemia exhibit changes in gut function leading to diarrhea as compared with a matched group of healthy calves.
AB  - Uvod. Neonatalna telad su često deficitarna u gvožđu. Brojni podaci ukazuju na povezanost nedostatka gvožđa sa različitim patološkim stanjima, uključujući i pojavu dijareje. Cilj rada bio je da se ispita povezanost statusa gvožđa i promena u funkciji digestivnog trakta novorođene teladi sa i bez znakova dijareje. Materijal i Metode. Telad su podeljena u dve grupe na osnovu pojave dijareje; prva grupa (n=6) telad sa dijarejom i druga grupa (n=6) klinički zdrava telad bez znakova dijareje. Uzorci krvi su uzeti od sve teladi 12. dana života i određeni su parametri crvene krvne slike, sadržaj serumskog gvožđa i ukupni kapacitet vezivanja gvožđa. Parametri crvene krvne slike određeni su automatskim hematološkim analajzerom, dok su biohemijski parametri određeni spektrofotometrijski. Uzorci fecesa uzeti su od sve teladi i izmerena je pH vrednost semikvantitativno pomoću test tračica. Bakteriološkim pregledom uzorci fecesa su ispitani na prisustvo enterotoksogenih Escherichia coli, Salmonella spp. i Clostridium perfringens vrsta; RT-PCR metodom ispitani su na prisustvo goveđeg rotavirusa, coronavirusa i virusa goveđe virusne dijareje, dok su mikroskopski pregledani na prisustvo Cryptosporidium parvum. Rezultati i zaključak. Promene u koncentraciji gvožđa dovele su do pojave značajnih promena u hematološkim indeksima teladi sa dijarejom i pojavi mikrocitne hipohromne anemije. Vrednost pH fecesa je bila značajno veća kod teladi sa dijarejom u odnosu na zdravu telad. Svi uzorci fecesa bili su negativni na ispitivane enteropatogene uzročnike. U poređenju sa zdravom teladi, pretpostavka je da kod teladi sa anemijom koja je uzrokovana deficitom gvožđa nastaju promene u funkciji digestivnog trakta koje dovode do pojave dijareje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Occurrence of neonatal diarrhea in calves with iron-deficiency anemia
T1  - Neonatalna dijareja kod teladi sa anemijom usled nedostatka gvožđa
VL  - 73
IS  - 1
SP  - 1
EP  - 9
DO  - 10.2298/VETGL181210011P
ER  - 

@article{
author = "Prodanović, Radiša and Nedić, Sreten and Radanović, Oliver and Milićević, Vesna and Vujanac, Ivan and Bojkovski, Jovan and Kureljušić, Branislav and Arsić, Sveta and Jovanović, Ljubomir and Kirovski, Danijela",
year = "2019",
abstract = "Introduction. Neonatal calves are often deficient in iron. Accumulating evidence indicates that iron status is associated with disease pathologies including diarrhea. Our objective was to examine the association between iron status and gut function in neonatal calves with and without a history of calf diarrhea. Materials and Methods. Calves were divided into two groups based on their history of diarrhea; the first group were diarrheic calves (n=6) and the second group were non-diarrheic healthy calves (n=6). Blood samples (n=12) were collected at day 12 of age and erythrogram determination and measurements of serum iron and total iron binding capacity were performed. Hematological values were measured using an automatic analyzer, and biochemical properties were determined spectrophotometrically. Fecal samples were obtained from all calves and pH measured using semi quantitative test strips as well as being examined by bacterial cultivation for enterotoxigenic Escherichia coli, Salmonella spp. and Clostridium perfringens, by RT-PCR for the presence of bovine rotavirus, bovine coronavirus and bovine viral diarrhea virus, and by microscopy for the presence of Cryptosporidium parvum. Results and Conclusions. There were significant iron-related changes for most hematological indices in diarrheic calves; and iron (Fe) deficiency and microcytic, hypochromic anemia were diagnosed. The pH of the feces was significantly higher in diarrheic calves than in the non-diarrheic healthy group (P lt 0.01). All fecal samples were negative for the analyzed enteric pathogens. According to the results obtained, calves experiencing iron deficiency anemia exhibit changes in gut function leading to diarrhea as compared with a matched group of healthy calves., Uvod. Neonatalna telad su često deficitarna u gvožđu. Brojni podaci ukazuju na povezanost nedostatka gvožđa sa različitim patološkim stanjima, uključujući i pojavu dijareje. Cilj rada bio je da se ispita povezanost statusa gvožđa i promena u funkciji digestivnog trakta novorođene teladi sa i bez znakova dijareje. Materijal i Metode. Telad su podeljena u dve grupe na osnovu pojave dijareje; prva grupa (n=6) telad sa dijarejom i druga grupa (n=6) klinički zdrava telad bez znakova dijareje. Uzorci krvi su uzeti od sve teladi 12. dana života i određeni su parametri crvene krvne slike, sadržaj serumskog gvožđa i ukupni kapacitet vezivanja gvožđa. Parametri crvene krvne slike određeni su automatskim hematološkim analajzerom, dok su biohemijski parametri određeni spektrofotometrijski. Uzorci fecesa uzeti su od sve teladi i izmerena je pH vrednost semikvantitativno pomoću test tračica. Bakteriološkim pregledom uzorci fecesa su ispitani na prisustvo enterotoksogenih Escherichia coli, Salmonella spp. i Clostridium perfringens vrsta; RT-PCR metodom ispitani su na prisustvo goveđeg rotavirusa, coronavirusa i virusa goveđe virusne dijareje, dok su mikroskopski pregledani na prisustvo Cryptosporidium parvum. Rezultati i zaključak. Promene u koncentraciji gvožđa dovele su do pojave značajnih promena u hematološkim indeksima teladi sa dijarejom i pojavi mikrocitne hipohromne anemije. Vrednost pH fecesa je bila značajno veća kod teladi sa dijarejom u odnosu na zdravu telad. Svi uzorci fecesa bili su negativni na ispitivane enteropatogene uzročnike. U poređenju sa zdravom teladi, pretpostavka je da kod teladi sa anemijom koja je uzrokovana deficitom gvožđa nastaju promene u funkciji digestivnog trakta koje dovode do pojave dijareje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Occurrence of neonatal diarrhea in calves with iron-deficiency anemia, Neonatalna dijareja kod teladi sa anemijom usled nedostatka gvožđa",
volume = "73",
number = "1",
pages = "1-9",
doi = "10.2298/VETGL181210011P"
}

Prodanović, R., Nedić, S., Radanović, O., Milićević, V., Vujanac, I., Bojkovski, J., Kureljušić, B., Arsić, S., Jovanović, L.,& Kirovski, D.. (2019). Occurrence of neonatal diarrhea in calves with iron-deficiency anemia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 73(1), 1-9.
https://doi.org/10.2298/VETGL181210011P

Prodanović R, Nedić S, Radanović O, Milićević V, Vujanac I, Bojkovski J, Kureljušić B, Arsić S, Jovanović L, Kirovski D. Occurrence of neonatal diarrhea in calves with iron-deficiency anemia. in Veterinarski Glasnik. 2019;73(1):1-9.
doi:10.2298/VETGL181210011P .

Prodanović, Radiša, Nedić, Sreten, Radanović, Oliver, Milićević, Vesna, Vujanac, Ivan, Bojkovski, Jovan, Kureljušić, Branislav, Arsić, Sveta, Jovanović, Ljubomir, Kirovski, Danijela, "Occurrence of neonatal diarrhea in calves with iron-deficiency anemia" in Veterinarski Glasnik, 73, no. 1 (2019):1-9,
https://doi.org/10.2298/VETGL181210011P . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Maksimović-Zorić, Jelena
AU  - Stanojević, Slobodan
AU  - Radanović, Oliver
AU  - Đurić, Boban
AU  - Plavšić, Budimir
AU  - Vasić, Ana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1958
AB  - Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females.
AB  - Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.
PB  - Scientific Veterinary Institute “Novi Sad”, Novi Sad
T2  - Archives of Veterinary Medicine
T1  - Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016
T1  - Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine
VL  - 10
IS  - 1
DO  - 10.46784/e-avm.v10i1.76
ER  - 

@article{
author = "Pavlović, Ivan and Veljović, Ljubiša and Milićević, Vesna and Maksimović-Zorić, Jelena and Stanojević, Slobodan and Radanović, Oliver and Đurić, Boban and Plavšić, Budimir and Vasić, Ana",
year = "2019",
abstract = "Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females., Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.",
publisher = "Scientific Veterinary Institute “Novi Sad”, Novi Sad",
journal = "Archives of Veterinary Medicine",
title = "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016, Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine",
volume = "10",
number = "1",
doi = "10.46784/e-avm.v10i1.76"
}

Pavlović, I., Veljović, L., Milićević, V., Maksimović-Zorić, J., Stanojević, S., Radanović, O., Đurić, B., Plavšić, B.,& Vasić, A.. (2019). Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”, Novi Sad., 10(1).
https://doi.org/10.46784/e-avm.v10i1.76

Pavlović I, Veljović L, Milićević V, Maksimović-Zorić J, Stanojević S, Radanović O, Đurić B, Plavšić B, Vasić A. Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine. 2019;10(1).
doi:10.46784/e-avm.v10i1.76 .

Pavlović, Ivan, Veljović, Ljubiša, Milićević, Vesna, Maksimović-Zorić, Jelena, Stanojević, Slobodan, Radanović, Oliver, Đurić, Boban, Plavšić, Budimir, Vasić, Ana, "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016" in Archives of Veterinary Medicine, 10, no. 1 (2019),
https://doi.org/10.46784/e-avm.v10i1.76 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Maksimović, Slobodan
AU  - Vujinović, Slobodan
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Jezdimirović, Nemanja
AU  - Žutić, Jadranka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1901
AB  - U ovom radu prikazan je slučaj krave simentalske rase, starosti dve godine i šest meseci kod koje je postojala sumnja na nodularni dermatitis, sredinom 2017 godine. Kliničkim pregledom ustanovljeni su brojni noduli veličine od nekoliko milimetara do približno 10 centimetara diseminovani dominantno po koži vimena i perineuma, od kojih su neki konfluirali i egzulcerisali. Opšte stanje krave je bilo nepromenjeno. Krava je prema rezultatima laboratorijskih analiza bila negativna na prisustvo antitela protiv virusa leukoze goveda, pokazala je negativnu reakciju pri tuberkulinizaciji i bila je vakcinisana protiv nodularnog dermatitisa. Nakon hirurške ekscizije kožnog čvora, uzorak je bio negativan na prisustvo genoma virusa nodularnog dermatitisa. Kako bismo ustanovili morfologiju kožne lezije, urađena je histopatološka analiza. Histopatološka analiza je pokazala infiltraciju dermisa i subkutisa brojnim mononukleranim ćelijama koje pokazuju ćelijsku atipiju. Tada je postavljena sumnja da se radi o kutanom limfomu. Nadalje, imunohistohemijsko ispitivanje je potvrdilo da se u infiltratu nalaze isključivo CD3 imunopozitivne ćelije što sugeriše da se radi o T-ćelijskom neepiteliotropnom limfomu
AB  - In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.
PB  - FVM
T2  - Veterinarski Glasnik
T1  - Cutaneous lymphoma in a cow - a case report
T1  - Kutani limfom kod krave – prikaz slučaja
VL  - 73
IS  - 1
SP  - 50
EP  - 56
DO  - 10.2298/VETGL180904004K
ER  - 

@article{
author = "Kureljušić, Branislav and Maksimović, Slobodan and Vujinović, Slobodan and Savić, Božidar and Milićević, Vesna and Veljović, Ljubiša and Jezdimirović, Nemanja and Žutić, Jadranka",
year = "2019",
abstract = "U ovom radu prikazan je slučaj krave simentalske rase, starosti dve godine i šest meseci kod koje je postojala sumnja na nodularni dermatitis, sredinom 2017 godine. Kliničkim pregledom ustanovljeni su brojni noduli veličine od nekoliko milimetara do približno 10 centimetara diseminovani dominantno po koži vimena i perineuma, od kojih su neki konfluirali i egzulcerisali. Opšte stanje krave je bilo nepromenjeno. Krava je prema rezultatima laboratorijskih analiza bila negativna na prisustvo antitela protiv virusa leukoze goveda, pokazala je negativnu reakciju pri tuberkulinizaciji i bila je vakcinisana protiv nodularnog dermatitisa. Nakon hirurške ekscizije kožnog čvora, uzorak je bio negativan na prisustvo genoma virusa nodularnog dermatitisa. Kako bismo ustanovili morfologiju kožne lezije, urađena je histopatološka analiza. Histopatološka analiza je pokazala infiltraciju dermisa i subkutisa brojnim mononukleranim ćelijama koje pokazuju ćelijsku atipiju. Tada je postavljena sumnja da se radi o kutanom limfomu. Nadalje, imunohistohemijsko ispitivanje je potvrdilo da se u infiltratu nalaze isključivo CD3 imunopozitivne ćelije što sugeriše da se radi o T-ćelijskom neepiteliotropnom limfomu, In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.",
publisher = "FVM",
journal = "Veterinarski Glasnik",
title = "Cutaneous lymphoma in a cow - a case report, Kutani limfom kod krave – prikaz slučaja",
volume = "73",
number = "1",
pages = "50-56",
doi = "10.2298/VETGL180904004K"
}

Kureljušić, B., Maksimović, S., Vujinović, S., Savić, B., Milićević, V., Veljović, L., Jezdimirović, N.,& Žutić, J.. (2019). Cutaneous lymphoma in a cow - a case report. in Veterinarski Glasnik
FVM., 73(1), 50-56.
https://doi.org/10.2298/VETGL180904004K

Kureljušić B, Maksimović S, Vujinović S, Savić B, Milićević V, Veljović L, Jezdimirović N, Žutić J. Cutaneous lymphoma in a cow - a case report. in Veterinarski Glasnik. 2019;73(1):50-56.
doi:10.2298/VETGL180904004K .

Kureljušić, Branislav, Maksimović, Slobodan, Vujinović, Slobodan, Savić, Božidar, Milićević, Vesna, Veljović, Ljubiša, Jezdimirović, Nemanja, Žutić, Jadranka, "Cutaneous lymphoma in a cow - a case report" in Veterinarski Glasnik, 73, no. 1 (2019):50-56,
https://doi.org/10.2298/VETGL180904004K . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Kureljušić, Jasna
AU  - Šekler, Milanko
AU  - Nešić, Ksenija
AU  - Veljović, Ljubiša
AU  - Zorić-Maksimović, Jelena
AU  - Radosavljević, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1657
AB  - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.
PB  - Springeropen, London
T2  - AMB EXPRESS
T1  - Molecular detection of black queen cell virus and Kashmir bee virus in honey
VL  - 8
SP  - 128
DO  - 10.1186/s13568-018-0655-7
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Zorić-Maksimović, Jelena and Radosavljević, Vladimir",
year = "2018",
abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.",
publisher = "Springeropen, London",
journal = "AMB EXPRESS",
title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey",
volume = "8",
pages = "128",
doi = "10.1186/s13568-018-0655-7"
}

Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Zorić-Maksimović, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS
Springeropen, London., 8, 128.
https://doi.org/10.1186/s13568-018-0655-7

Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Zorić-Maksimović J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS. 2018;8:128.
doi:10.1186/s13568-018-0655-7 .

Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Zorić-Maksimović, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB EXPRESS, 8 (2018):128,
https://doi.org/10.1186/s13568-018-0655-7 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Radosavljević, Vladimir
AU  - Jezdimirović, Nemanja
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1564
AB  - Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever.
AB  - Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Some adverse events following immunization in veterinary medicine
T1  - Procena neželjenih reakcija nakon vakcinacije u veterini
VL  - 11
IS  - 1
SP  - 67
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1564
ER  - 

@article{
author = "Milićević, Vesna and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Žutić, Jadranka and Kureljušić, Branislav and Radosavljević, Vladimir and Jezdimirović, Nemanja and Radojičić, Sonja",
year = "2018",
abstract = "Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever., Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Some adverse events following immunization in veterinary medicine, Procena neželjenih reakcija nakon vakcinacije u veterini",
volume = "11",
number = "1",
pages = "67-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1564"
}

Milićević, V., Veljović, L., Maksimović-Zorić, J., Žutić, J., Kureljušić, B., Radosavljević, V., Jezdimirović, N.,& Radojičić, S.. (2018). Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 11(1), 67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564

Milićević V, Veljović L, Maksimović-Zorić J, Žutić J, Kureljušić B, Radosavljević V, Jezdimirović N, Radojičić S. Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine. 2018;11(1):67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

Milićević, Vesna, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Žutić, Jadranka, Kureljušić, Branislav, Radosavljević, Vladimir, Jezdimirović, Nemanja, Radojičić, Sonja, "Some adverse events following immunization in veterinary medicine" in Arhiv veterinarske medicine, 11, no. 1 (2018):67-77,
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radosavljević, Vladimir
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1450
AB  - African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia.
AB  - Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Epizootiological situation of African swine fever in Europe
T1  - Epizootiološka situacija afričke kuge svinja u Evropi
VL  - 10
IS  - 2
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1450
ER  - 

@article{
author = "Milićević, Vesna and Radosavljević, Vladimir and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Radojičić, Sonja",
year = "2017",
abstract = "African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia., Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Epizootiological situation of African swine fever in Europe, Epizootiološka situacija afričke kuge svinja u Evropi",
volume = "10",
number = "2",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1450"
}

Milićević, V., Radosavljević, V., Veljović, L., Maksimović-Zorić, J.,& Radojičić, S.. (2017). Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 10(2), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450

Milićević V, Radosavljević V, Veljović L, Maksimović-Zorić J, Radojičić S. Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine. 2017;10(2):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .

Milićević, Vesna, Radosavljević, Vladimir, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Radojičić, Sonja, "Epizootiological situation of African swine fever in Europe" in Arhiv veterinarske medicine, 10, no. 2 (2017):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .