TY  - JOUR
AU  - Blažić, Tanja
AU  - Stojnić, Bojan
AU  - Milanović, Svetlana
AU  - Jokić, Goran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3867
AB  - In addition to having a negative impact on the health of people and domestic animals, rodents
often cause enormous damage to the environment by disrupting natural biodiversity. The negative impacts of rodents in urban and rural areas have required intensive use of rodentcides in spite
of the proven risk of secondary poisoning of non-target predators and scavengers. Continuous and
intensive use of rodenticides has led to environmental pollution through their retention in the
environment. Commensal rodents are predominantly managed with anticoagulant rodenticides,
which are very persistent in the environment and move up the food chain and accumulate in the
bodies of predators and scavengers. Generally, the use of anticoagulant rodenticides continues,
and there is a need to take appropriate measures to reduce their harmful impact. The efficacy of
second generation anticoagulants (bromadiolone, difenacoum and brodifacoum), combined
either mutually or with chlorophacinone at reduced doses (0.001 % and 0.0008 %), in controlling
brown rats (Rattus norvegicus) was tested in a four-day no-choice feeding test. Combinations of
second generation anticoagulants were more effective than the combination of chlorophacinone
and second generation anticoagulants. The results indicate that combinations of different anticoagulants at multifold lower doses than the standard may provide a successful tool for brown rat
control and a more environment-friendly method of rodent control and protection of non-target
animals.
PB  - Elsevier
T2  - Heliyon
T1  - A strategy to improve rodent control while reducing rodenticide release into the environment
VL  - 10
IS  - 8
SP  - e29471
DO  - 10.1016/j.heliyon.2024.e29471
ER  - 

@article{
author = "Blažić, Tanja and Stojnić, Bojan and Milanović, Svetlana and Jokić, Goran",
year = "2024",
abstract = "In addition to having a negative impact on the health of people and domestic animals, rodents
often cause enormous damage to the environment by disrupting natural biodiversity. The negative impacts of rodents in urban and rural areas have required intensive use of rodentcides in spite
of the proven risk of secondary poisoning of non-target predators and scavengers. Continuous and
intensive use of rodenticides has led to environmental pollution through their retention in the
environment. Commensal rodents are predominantly managed with anticoagulant rodenticides,
which are very persistent in the environment and move up the food chain and accumulate in the
bodies of predators and scavengers. Generally, the use of anticoagulant rodenticides continues,
and there is a need to take appropriate measures to reduce their harmful impact. The efficacy of
second generation anticoagulants (bromadiolone, difenacoum and brodifacoum), combined
either mutually or with chlorophacinone at reduced doses (0.001 % and 0.0008 %), in controlling
brown rats (Rattus norvegicus) was tested in a four-day no-choice feeding test. Combinations of
second generation anticoagulants were more effective than the combination of chlorophacinone
and second generation anticoagulants. The results indicate that combinations of different anticoagulants at multifold lower doses than the standard may provide a successful tool for brown rat
control and a more environment-friendly method of rodent control and protection of non-target
animals.",
publisher = "Elsevier",
journal = "Heliyon",
title = "A strategy to improve rodent control while reducing rodenticide release into the environment",
volume = "10",
number = "8",
pages = "e29471",
doi = "10.1016/j.heliyon.2024.e29471"
}

Blažić, T., Stojnić, B., Milanović, S.,& Jokić, G.. (2024). A strategy to improve rodent control while reducing rodenticide release into the environment. in Heliyon
Elsevier., 10(8), e29471.
https://doi.org/10.1016/j.heliyon.2024.e29471

Blažić T, Stojnić B, Milanović S, Jokić G. A strategy to improve rodent control while reducing rodenticide release into the environment. in Heliyon. 2024;10(8):e29471.
doi:10.1016/j.heliyon.2024.e29471 .

Blažić, Tanja, Stojnić, Bojan, Milanović, Svetlana, Jokić, Goran, "A strategy to improve rodent control while reducing rodenticide release into the environment" in Heliyon, 10, no. 8 (2024):e29471,
https://doi.org/10.1016/j.heliyon.2024.e29471 . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3085
AB  - Током раста бројлера долази до адаптивних промена антиоксидативног
статуса. Глутатион пероксидаза (GPx) је један од ензима који штите ћелије од
оксидативног стреса, а малондиалдехид (МДА) настаје током пероксидације
фослипида ћелијске мембране и поуздан је показатељ степена оксидативног
оштећења.
Циљ овог рада је био да се одреде промене активности GPx и концентрације
МДА током прве 4 недеље това (1., 14. и 28. дан), као и ефекат дијетарне
суплементације алфа-токоферолом (100 IU/kg хране).
Оглед је спроведен на товним пилићима старости од 1 дан до 28. дана това.
Пилад су била подељена у 2 огледне групе. Прва група тј. kонтрола (н=10) је
храњена стандардном смешом која задовољава све нутритивне потребе, док је
друга огледна група (н=10) добијала исту храну уз додатак 100 IU/kg хране
алфа-токоферола. У назначеним данима узимани су узорци хепаринисане
крви пункцијом крилне вене из које је у року од 2 часа издвојена крвна плазма,
у којој су одређивани GPx и МДА.
GPx мерена је куплованим тестом (Gunzler и сар. 1974). Принцип мерења је
базиран на спектрофотометријском регистровању утрошка NADPH у
куплованом ензимском систему. Малондиалдехид (МДА) је одређен методом
описаном од стране Андрееве, 1988.
Просечне вредности GPx су се кретале у интервалу од 21,46 до 38,55 µкат/л.
Најниже просечне вредности су забележене 14. дана това, док су највише
просечне вредности забележене 28. дана. Међутим, вредности активности GPx
се нису статистички разликовале (п>0,05), нити између контролне групе и
групе суплементиране алфа-токоферолом, нити приликом поређења група
током посматраног временског периода. Вредности МДА су биле најниже 14.
дана , при чему је контролна група имала сигнификантно (п<0,01) више
вредности МДА у односу на групу суплементирану алфа-токоферолом.
Значајан пораст концентрације МДА у плазми бројлера је забележен 28. дана
това, при чему су вредности концентрације у обе групе скоро утрострућене.
Несуплементирана група је имала сигнификантно веће вредности (п<0,05)
концентрације МДА у односу на суплементирану групу.
Резултати указују да током прве 4 недеље това долази до значајних промена
показатеља антиоксидативне заштите и нивоа пероксидације мембранских фосфолипида. Ефекат суплементираног алфа-токоферола је значајан са
аспекта смањења концентрације МДА у плазми.
AB  - In broilers during the growth stage, adaptive changes in antioxidant status occur.
Glutathione peroxidase (GPx) is one of the enzymes that protect cells from
oxidative stress, and malondialdehyde (MDA) is formed during the peroxidation
of cell membrane phospholipids and is a reliable indicator of the degree of
oxidative damage.
The aim of this work was to determine changes in GPx activity and MDA
concentration during the first 4 weeks of growth (1st, 14th and 28th day), as well
as the effects of dietary supplementation with alpha-tocopherol (100 IU/kg feed)
on the above parameters.
The experiment was conducted on broiler chickens aged from 1 day to 28 days of
fattening. The chicks were divided into 2 experimental groups. The first i.e.
control group (n=10) was fed a standard mixture that meets the needs, while the
second experimental group (n=10) received the same mixture with the addition
of 100 IU/kg of feed alpha-tocopherol. On the indicated days, samples of
heparinized blood were taken by puncture of the wing vein, from which blood
plasma was separated within 2 hours, in which GPx and MDA were determined.
GPx was measured by the coupled test (Gunzler et al. 1974). The test is based on
the spectrophotometric registration of NADPH consumption in the coupled
enzyme system. Malondialdehyde (MDA) was determined based on
spectrophotometric light absorption of the resulting stable pink solution, using
the method described by Andreeva, 1988.
The average values obtained for GPx ranged from 21.46 to 38.55 µkat/l. The lowest
average values were recorded on the 14th day of fattening, while the highest
average values were recorded on the 28th day. However, GPx activity values were
not statistically significantly different (p>0.05), neither between the control
group and the group supplemented with alpha-tocopherol, nor when comparing
the groups during the observed time period. MDA values were the lowest on day
14, where the control group had significantly (p<0.01) higher MDA values
compared to the group supplemented with alpha-tocopherol. A significant
increase in the concentration of MDA in broiler plasma was recorded on the 28th
day of fattening, where the concentration values in both groups were almost
tripled. The non-supplemented group had significantly higher values (p<0.05) of
MDA concentration compared to the supplemented group.
The results indicate that during the first 4 weeks of fattening, there are significant
changes in the indicators of antioxidant protection and the level of peroxidation of membrane phospholipids. The effect of supplemented alpha-tocopherol is
significant from the point of view of reducing the concentration of MDA in
plasma.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera
T1  - Influence of alpha-tocopherol on GPx activity and MDA concentration in the blood plasma of broilers
SP  - 179
EP  - 182
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3085
ER  - 

@conference{
author = "Valčić, Olivera and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2023",
abstract = "Током раста бројлера долази до адаптивних промена антиоксидативног
статуса. Глутатион пероксидаза (GPx) је један од ензима који штите ћелије од
оксидативног стреса, а малондиалдехид (МДА) настаје током пероксидације
фослипида ћелијске мембране и поуздан је показатељ степена оксидативног
оштећења.
Циљ овог рада је био да се одреде промене активности GPx и концентрације
МДА током прве 4 недеље това (1., 14. и 28. дан), као и ефекат дијетарне
суплементације алфа-токоферолом (100 IU/kg хране).
Оглед је спроведен на товним пилићима старости од 1 дан до 28. дана това.
Пилад су била подељена у 2 огледне групе. Прва група тј. kонтрола (н=10) је
храњена стандардном смешом која задовољава све нутритивне потребе, док је
друга огледна група (н=10) добијала исту храну уз додатак 100 IU/kg хране
алфа-токоферола. У назначеним данима узимани су узорци хепаринисане
крви пункцијом крилне вене из које је у року од 2 часа издвојена крвна плазма,
у којој су одређивани GPx и МДА.
GPx мерена је куплованим тестом (Gunzler и сар. 1974). Принцип мерења је
базиран на спектрофотометријском регистровању утрошка NADPH у
куплованом ензимском систему. Малондиалдехид (МДА) је одређен методом
описаном од стране Андрееве, 1988.
Просечне вредности GPx су се кретале у интервалу од 21,46 до 38,55 µкат/л.
Најниже просечне вредности су забележене 14. дана това, док су највише
просечне вредности забележене 28. дана. Међутим, вредности активности GPx
се нису статистички разликовале (п>0,05), нити између контролне групе и
групе суплементиране алфа-токоферолом, нити приликом поређења група
током посматраног временског периода. Вредности МДА су биле најниже 14.
дана , при чему је контролна група имала сигнификантно (п<0,01) више
вредности МДА у односу на групу суплементирану алфа-токоферолом.
Значајан пораст концентрације МДА у плазми бројлера је забележен 28. дана
това, при чему су вредности концентрације у обе групе скоро утрострућене.
Несуплементирана група је имала сигнификантно веће вредности (п<0,05)
концентрације МДА у односу на суплементирану групу.
Резултати указују да током прве 4 недеље това долази до значајних промена
показатеља антиоксидативне заштите и нивоа пероксидације мембранских фосфолипида. Ефекат суплементираног алфа-токоферола је значајан са
аспекта смањења концентрације МДА у плазми., In broilers during the growth stage, adaptive changes in antioxidant status occur.
Glutathione peroxidase (GPx) is one of the enzymes that protect cells from
oxidative stress, and malondialdehyde (MDA) is formed during the peroxidation
of cell membrane phospholipids and is a reliable indicator of the degree of
oxidative damage.
The aim of this work was to determine changes in GPx activity and MDA
concentration during the first 4 weeks of growth (1st, 14th and 28th day), as well
as the effects of dietary supplementation with alpha-tocopherol (100 IU/kg feed)
on the above parameters.
The experiment was conducted on broiler chickens aged from 1 day to 28 days of
fattening. The chicks were divided into 2 experimental groups. The first i.e.
control group (n=10) was fed a standard mixture that meets the needs, while the
second experimental group (n=10) received the same mixture with the addition
of 100 IU/kg of feed alpha-tocopherol. On the indicated days, samples of
heparinized blood were taken by puncture of the wing vein, from which blood
plasma was separated within 2 hours, in which GPx and MDA were determined.
GPx was measured by the coupled test (Gunzler et al. 1974). The test is based on
the spectrophotometric registration of NADPH consumption in the coupled
enzyme system. Malondialdehyde (MDA) was determined based on
spectrophotometric light absorption of the resulting stable pink solution, using
the method described by Andreeva, 1988.
The average values obtained for GPx ranged from 21.46 to 38.55 µkat/l. The lowest
average values were recorded on the 14th day of fattening, while the highest
average values were recorded on the 28th day. However, GPx activity values were
not statistically significantly different (p>0.05), neither between the control
group and the group supplemented with alpha-tocopherol, nor when comparing
the groups during the observed time period. MDA values were the lowest on day
14, where the control group had significantly (p<0.01) higher MDA values
compared to the group supplemented with alpha-tocopherol. A significant
increase in the concentration of MDA in broiler plasma was recorded on the 28th
day of fattening, where the concentration values in both groups were almost
tripled. The non-supplemented group had significantly higher values (p<0.05) of
MDA concentration compared to the supplemented group.
The results indicate that during the first 4 weeks of fattening, there are significant
changes in the indicators of antioxidant protection and the level of peroxidation of membrane phospholipids. The effect of supplemented alpha-tocopherol is
significant from the point of view of reducing the concentration of MDA in
plasma.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera, Influence of alpha-tocopherol on GPx activity and MDA concentration in the blood plasma of broilers",
pages = "179-182",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3085"
}

Valčić, O., Jovanović, I. B.,& Milanović, S.. (2023). Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 179-182.
https://hdl.handle.net/21.15107/rcub_veterinar_3085

Valčić O, Jovanović IB, Milanović S. Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:179-182.
https://hdl.handle.net/21.15107/rcub_veterinar_3085 .

Valčić, Olivera, Jovanović, Ivan B., Milanović, Svetlana, "Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):179-182,
https://hdl.handle.net/21.15107/rcub_veterinar_3085 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Jovanović, Ivan B.
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3107
AB  - Gastrointestinalni trakt predstavlja kompleksan ekosistem koji sadrži veliki broj
metabolički aktivnih mikroorganizama. Među značajnim metabolitima mikrobioma posebno se
ističu masne kiseline kratkih lanaca (SCFA), sekundarne žučne kiseline, poliamini i indol, zajedno
sa neurotransmiterima kao što su gama-aminobutirat, serotonin i kateholamini. Brojni radovi
dokazuju uticaj metabolita mikrobioma na rast, prirast, stres i imunske reakcije organizma.
Međutim, tek u novijim radovima se ukazuje na činjenicu da ne utiče samo mikrobiom svojim
metabolitima na zdrastveni status svinja, već da postoje znatno složenije interakcije između
domaćina i mikrobioma. Ovom prilikom, želimo da istaknemo ne samo već poznat značaj
mikrobioma za zdravlje i performanse svinja, već i najnovija naučna saznanja iz oblasti složenih
interakcija mikrobiom-domaćin (svinja)- mikrobiom- crevno/nervna osovina (gut brain axis).
Cilj ovog rada je da na slikovit i razumljiv način objasni najnovija saznanja iz oblasti
složenih metaboličkih interakcija koje postoje između mikrobioma, hrane, crevno-moždane
osovine i domaćina kako bi se implementirala u intenzivnom farmskom uzgoju svinja u cilju
poboljšanja dobrobiti i zdravlja životinja.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
T1  - Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja
SP  - 158
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3107
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana and Jovanović, Ivan B.",
year = "2023",
abstract = "Gastrointestinalni trakt predstavlja kompleksan ekosistem koji sadrži veliki broj
metabolički aktivnih mikroorganizama. Među značajnim metabolitima mikrobioma posebno se
ističu masne kiseline kratkih lanaca (SCFA), sekundarne žučne kiseline, poliamini i indol, zajedno
sa neurotransmiterima kao što su gama-aminobutirat, serotonin i kateholamini. Brojni radovi
dokazuju uticaj metabolita mikrobioma na rast, prirast, stres i imunske reakcije organizma.
Međutim, tek u novijim radovima se ukazuje na činjenicu da ne utiče samo mikrobiom svojim
metabolitima na zdrastveni status svinja, već da postoje znatno složenije interakcije između
domaćina i mikrobioma. Ovom prilikom, želimo da istaknemo ne samo već poznat značaj
mikrobioma za zdravlje i performanse svinja, već i najnovija naučna saznanja iz oblasti složenih
interakcija mikrobiom-domaćin (svinja)- mikrobiom- crevno/nervna osovina (gut brain axis).
Cilj ovog rada je da na slikovit i razumljiv način objasni najnovija saznanja iz oblasti
složenih metaboličkih interakcija koje postoje između mikrobioma, hrane, crevno-moždane
osovine i domaćina kako bi se implementirala u intenzivnom farmskom uzgoju svinja u cilju
poboljšanja dobrobiti i zdravlja životinja.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023",
title = "Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja",
pages = "158-164",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3107"
}

Valčić, O., Milanović, S.,& Jovanović, I. B.. (2023). Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
Požarevac : Sitograf RM., 158-164.
https://hdl.handle.net/21.15107/rcub_veterinar_3107

Valčić O, Milanović S, Jovanović IB. Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023. 2023;:158-164.
https://hdl.handle.net/21.15107/rcub_veterinar_3107 .

Valčić, Olivera, Milanović, Svetlana, Jovanović, Ivan B., "Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023 (2023):158-164,
https://hdl.handle.net/21.15107/rcub_veterinar_3107 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Gvozdić, Dragan
AU  - Jovanović, Ivan B.
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2418
AB  - U intenzivnoj stočarskoj proizvodnji, od muznih krava se očekuje maksimalno iskorišćavanje genetskog potencijala i što veći prinos
mleka. Najveći izazov u tom smislu predstavlja peripartalni period koji obuhvata tri nedelje pre i tri nedelje posle partusa. Tada se u organizmu krave dešavaju velike hormonske, metaboličke i imunološke
promene. Mnogi metabolički poremećaji koji su karakteristični za ovaj
period, posledica su neizbalansirane ishrane i nepravilne pripreme jedinke za ulazak u intenzivnu laktaciju. Masna jetra, ketoza, dislokacija
sirišta i puerperalna pareza predstavljaju glavne poremećaje karakteristične za ovaj period, a oksidativni stres može imati veliku ulogu u
njihovoj pojavi. Osim navedenih poremećaja, zaostajanje posteljice i
pojava mastitisa, mogu takođe biti u vezi sa oksidativnim stresom. U
ovom radu ćemo analizirati podatke iz naših istraživanja i dostupne literature i pokušati da objasnimo ulogu oksidativnog stresa u peripartalnom periodu, sa posebnim osvrtom na primenu antioksidanasa u cilju preveniranja pojave ili posledica metaboličkih poremećaja.
AB  - In intensive production, dairy cows are expected to make the most of their genetic
potential and maximize milk yield. The biggest challenge in this regard is the peripartal
period, which includes three weeks before and three weeks after parturition. In that interval
major hormonal, metabolic and immune changes occur in the cow’s body. Many metabolic
disorders that are characteristic of this period are the result of an unbalanced diet and
improper preparation of the individual to enter intensive lactation. Fatty liver, ketosis,
dislocation of the abomasum, and puerperal paresis are the main disorders which are
characteristic of this period, and oxidative stress can play a major role in their occurrence.
In addition to these disorders, retained placenta and mastitis may also be related to
oxidative stress. In this paper, we will analyze the data from our research and the available literature, and try to explain the role of oxidative stress in the peripartal period, with special
emphasis on the use of antioxidants in the prevention of the occurrence or consequences
of metabolic disorders.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja
T1  - Oxidative stress in cows in the peripartal period – cause or consequence of metabolic disorders
SP  - 67
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2418
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Gvozdić, Dragan and Jovanović, Ivan B.",
year = "2022",
abstract = "U intenzivnoj stočarskoj proizvodnji, od muznih krava se očekuje maksimalno iskorišćavanje genetskog potencijala i što veći prinos
mleka. Najveći izazov u tom smislu predstavlja peripartalni period koji obuhvata tri nedelje pre i tri nedelje posle partusa. Tada se u organizmu krave dešavaju velike hormonske, metaboličke i imunološke
promene. Mnogi metabolički poremećaji koji su karakteristični za ovaj
period, posledica su neizbalansirane ishrane i nepravilne pripreme jedinke za ulazak u intenzivnu laktaciju. Masna jetra, ketoza, dislokacija
sirišta i puerperalna pareza predstavljaju glavne poremećaje karakteristične za ovaj period, a oksidativni stres može imati veliku ulogu u
njihovoj pojavi. Osim navedenih poremećaja, zaostajanje posteljice i
pojava mastitisa, mogu takođe biti u vezi sa oksidativnim stresom. U
ovom radu ćemo analizirati podatke iz naših istraživanja i dostupne literature i pokušati da objasnimo ulogu oksidativnog stresa u peripartalnom periodu, sa posebnim osvrtom na primenu antioksidanasa u cilju preveniranja pojave ili posledica metaboličkih poremećaja., In intensive production, dairy cows are expected to make the most of their genetic
potential and maximize milk yield. The biggest challenge in this regard is the peripartal
period, which includes three weeks before and three weeks after parturition. In that interval
major hormonal, metabolic and immune changes occur in the cow’s body. Many metabolic
disorders that are characteristic of this period are the result of an unbalanced diet and
improper preparation of the individual to enter intensive lactation. Fatty liver, ketosis,
dislocation of the abomasum, and puerperal paresis are the main disorders which are
characteristic of this period, and oxidative stress can play a major role in their occurrence.
In addition to these disorders, retained placenta and mastitis may also be related to
oxidative stress. In this paper, we will analyze the data from our research and the available literature, and try to explain the role of oxidative stress in the peripartal period, with special
emphasis on the use of antioxidants in the prevention of the occurrence or consequences
of metabolic disorders.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja, Oxidative stress in cows in the peripartal period – cause or consequence of metabolic disorders",
pages = "67-78",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2418"
}

Milanović, S., Valčić, O., Gvozdić, D.,& Jovanović, I. B.. (2022). Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 67-78.
https://hdl.handle.net/21.15107/rcub_veterinar_2418

Milanović S, Valčić O, Gvozdić D, Jovanović IB. Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:67-78.
https://hdl.handle.net/21.15107/rcub_veterinar_2418 .

Milanović, Svetlana, Valčić, Olivera, Gvozdić, Dragan, Jovanović, Ivan B., "Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):67-78,
https://hdl.handle.net/21.15107/rcub_veterinar_2418 .

TY  - CONF
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Janjić, Filip
AU  - Francuski Andrić, Jelena
AU  - Beletić, Anđelo
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2612
C3  - 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022
T1  - Oxidative stress in canine babesiosis
SP  - 80
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2612
ER  - 

@conference{
author = "Radaković, Milena and Spariosu, Kristina and Janjić, Filip and Francuski Andrić, Jelena and Beletić, Anđelo and Milanović, Svetlana and Kovačević Filipović, Milica",
year = "2022",
journal = "24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022",
title = "Oxidative stress in canine babesiosis",
pages = "80-81",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2612"
}

Radaković, M., Spariosu, K., Janjić, F., Francuski Andrić, J., Beletić, A., Milanović, S.,& Kovačević Filipović, M.. (2022). Oxidative stress in canine babesiosis. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022, 80-81.
https://hdl.handle.net/21.15107/rcub_veterinar_2612

Radaković M, Spariosu K, Janjić F, Francuski Andrić J, Beletić A, Milanović S, Kovačević Filipović M. Oxidative stress in canine babesiosis. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022. 2022;:80-81.
https://hdl.handle.net/21.15107/rcub_veterinar_2612 .

Radaković, Milena, Spariosu, Kristina, Janjić, Filip, Francuski Andrić, Jelena, Beletić, Anđelo, Milanović, Svetlana, Kovačević Filipović, Milica, "Oxidative stress in canine babesiosis" in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 2022 (2022):80-81,
https://hdl.handle.net/21.15107/rcub_veterinar_2612 .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milosavljević, Petar
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3373
AB  - Глутатион пероксидаза (GPx) је ензим који постоји у 8 изоензимских
облика, од којих GPx-1 је облигаторни интрацелуларни ензим, док је GPx-3
активна у екстрацелуларним течностима, посебно у крвној плазми. Основна
улога GPx се огледа у заштити ћелија од оксидативног стреса којег индукују
слободни кисеонични радикали. Активност GPx се узима као поуздан
индикатор статуса селена у организму људи и животиња. Основни пут уноса
селена у организам је преко ланца исхране. Унети селен се уграђује у облику
селенометионина и селеноцистеина у ткивне протеине, односно ензиме. У
Србији су извршена темељна испитивања садржаја селена у хранивима и
домаћим животињама, посебно код економски значајних врста као што су
живина, свиње, овце и говеда. Међутим, до сада нису детаљно испитани
радни хладнокрвни коњи, посебно они који се хране искључиво локално
узгајаним хранивима, или су на испаши. Услед специфичног начина држања
они су идеални индикатори статуса селена моногастричних биљоједа на
датом локалитету.
Циљ нашег истраживања је био да одредимо статус селена на основу
активности GPx-1 и GPx -3 у узорцима крви несуплементираних радних
коња на територији централне Србије.
У нашем истраживању укупно је испитано 12 узорака крви коња преузетих
са локалитета општина Краљево, Зајечар, Ваљево и Димитровград, и то: 12
узорака крвне плазме и 12 узорака испраних еритроцита. Мерење
активности GPx-3 и GPx-1 је вршено методом по Гунцлеру на таласној
дужини од 366nm. Приликом узорковања за свако грло су евидентирани
подаци о полу, старости, вакцинацији и дехелминтизацији, саставу и
пореклу датих хранива. Просечна активност GPx-1 је 502,02 ± 91,77 μKat/l , а GPx-3 3,46 ± 1,02
μKat/l што указује на постојање маргиналног дефицита селена у популацији
несуплементираних радних коња на територији централне Србије.
AB  - Glutathione peroxidase (GPx) is an enzyme that has 8 isoenzyme forms, of which
GPx-1 is an obligatory intracellular enzyme, while GPx-3 is active in extracellular
fluids, especially in blood plasma. The main role of GPx is the protection of cells
from oxidative stress induced by free oxygen radicals. GPx activity is taken as a
reliable indicator of selenium status in the human and animal organism. Selenium
is introduced in organism mainly through the food chain. Ingested selenium is
incorporated in the form of selenomethionine and selenocysteine into tissue
proteins, i.e. enzymes. Detailed investigation related to the content of selenium in
feed and domestic animals were carried out in Serbia, especially in economically
significant species such as poultry, pigs, sheep and cattle. However, working coldblooded
horses, especially those that are fed exclusively with locally grown feed,
or are on pasture, have not been examined in detail so far. Due to their specific
way of breeding, they are ideal indicators of the selenium status of monogastric
herbivores in a given locality.
The goal of our study was to determine the status of selenium based on the
activity of GPx-1 and GPx-3 in blood samples of non-supplemented working
horses in the territory of central Serbia.
In our study, a total of 12 samples of horse blood taken from the localities of the
municipalities of Kraljevo, Zaječar, Valjevo and Dimitrovgrad were tested as
follows: 12 samples of blood plasma and 12 samples of washed erythrocytes.
Measurement of GPx-3 and GPx-1 activity was carried out using the Guncler
method at a wavelength of 366 nm. For each animal, during sampling, data on
gender, age, vaccination and deworming, composition and origin of the given
nutrients were recorded. The average activity of GPx-1 was 502.02 ± 91.77 μKat/l, and GPx-3 3.46 ± 1.02
μKat/l, which indicates the existence of a marginal selenium deficit in the
population of unsupplemented working horses in the territory of central Serbia.
PB  - Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'
T2  - Veterinary Journal of Republic of Srpska
T1  - Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije
T1  - Activity of selenenzymes GPx-1 and GPx-3 in the blood of working horses in the territory of central Serbia
VL  - 22
IS  - 1-2
SP  - 178
EP  - 193
DO  - 10.7251/VETJSR2201178V
DO  - 10.7251/VETJEN2201186V
ER  - 

@article{
author = "Valčić, Olivera and Milosavljević, Petar and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2022",
abstract = "Глутатион пероксидаза (GPx) је ензим који постоји у 8 изоензимских
облика, од којих GPx-1 је облигаторни интрацелуларни ензим, док је GPx-3
активна у екстрацелуларним течностима, посебно у крвној плазми. Основна
улога GPx се огледа у заштити ћелија од оксидативног стреса којег индукују
слободни кисеонични радикали. Активност GPx се узима као поуздан
индикатор статуса селена у организму људи и животиња. Основни пут уноса
селена у организам је преко ланца исхране. Унети селен се уграђује у облику
селенометионина и селеноцистеина у ткивне протеине, односно ензиме. У
Србији су извршена темељна испитивања садржаја селена у хранивима и
домаћим животињама, посебно код економски значајних врста као што су
живина, свиње, овце и говеда. Међутим, до сада нису детаљно испитани
радни хладнокрвни коњи, посебно они који се хране искључиво локално
узгајаним хранивима, или су на испаши. Услед специфичног начина држања
они су идеални индикатори статуса селена моногастричних биљоједа на
датом локалитету.
Циљ нашег истраживања је био да одредимо статус селена на основу
активности GPx-1 и GPx -3 у узорцима крви несуплементираних радних
коња на територији централне Србије.
У нашем истраживању укупно је испитано 12 узорака крви коња преузетих
са локалитета општина Краљево, Зајечар, Ваљево и Димитровград, и то: 12
узорака крвне плазме и 12 узорака испраних еритроцита. Мерење
активности GPx-3 и GPx-1 је вршено методом по Гунцлеру на таласној
дужини од 366nm. Приликом узорковања за свако грло су евидентирани
подаци о полу, старости, вакцинацији и дехелминтизацији, саставу и
пореклу датих хранива. Просечна активност GPx-1 је 502,02 ± 91,77 μKat/l , а GPx-3 3,46 ± 1,02
μKat/l што указује на постојање маргиналног дефицита селена у популацији
несуплементираних радних коња на територији централне Србије., Glutathione peroxidase (GPx) is an enzyme that has 8 isoenzyme forms, of which
GPx-1 is an obligatory intracellular enzyme, while GPx-3 is active in extracellular
fluids, especially in blood plasma. The main role of GPx is the protection of cells
from oxidative stress induced by free oxygen radicals. GPx activity is taken as a
reliable indicator of selenium status in the human and animal organism. Selenium
is introduced in organism mainly through the food chain. Ingested selenium is
incorporated in the form of selenomethionine and selenocysteine into tissue
proteins, i.e. enzymes. Detailed investigation related to the content of selenium in
feed and domestic animals were carried out in Serbia, especially in economically
significant species such as poultry, pigs, sheep and cattle. However, working coldblooded
horses, especially those that are fed exclusively with locally grown feed,
or are on pasture, have not been examined in detail so far. Due to their specific
way of breeding, they are ideal indicators of the selenium status of monogastric
herbivores in a given locality.
The goal of our study was to determine the status of selenium based on the
activity of GPx-1 and GPx-3 in blood samples of non-supplemented working
horses in the territory of central Serbia.
In our study, a total of 12 samples of horse blood taken from the localities of the
municipalities of Kraljevo, Zaječar, Valjevo and Dimitrovgrad were tested as
follows: 12 samples of blood plasma and 12 samples of washed erythrocytes.
Measurement of GPx-3 and GPx-1 activity was carried out using the Guncler
method at a wavelength of 366 nm. For each animal, during sampling, data on
gender, age, vaccination and deworming, composition and origin of the given
nutrients were recorded. The average activity of GPx-1 was 502.02 ± 91.77 μKat/l, and GPx-3 3.46 ± 1.02
μKat/l, which indicates the existence of a marginal selenium deficit in the
population of unsupplemented working horses in the territory of central Serbia.",
publisher = "Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'",
journal = "Veterinary Journal of Republic of Srpska",
title = "Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije, Activity of selenenzymes GPx-1 and GPx-3 in the blood of working horses in the territory of central Serbia",
volume = "22",
number = "1-2",
pages = "178-193",
doi = "10.7251/VETJSR2201178V, 10.7251/VETJEN2201186V"
}

Valčić, O., Milosavljević, P., Jovanović, I. B.,& Milanović, S.. (2022). Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije. in Veterinary Journal of Republic of Srpska
Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'., 22(1-2), 178-193.
https://doi.org/10.7251/VETJSR2201178V

Valčić O, Milosavljević P, Jovanović IB, Milanović S. Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije. in Veterinary Journal of Republic of Srpska. 2022;22(1-2):178-193.
doi:10.7251/VETJSR2201178V .

Valčić, Olivera, Milosavljević, Petar, Jovanović, Ivan B., Milanović, Svetlana, "Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije" in Veterinary Journal of Republic of Srpska, 22, no. 1-2 (2022):178-193,
https://doi.org/10.7251/VETJSR2201178V . .

TY  - JOUR
AU  - Spariosu, Kristina
AU  - Janjić, Filip
AU  - Francuski Andrić, Jelena
AU  - Radaković, Milena
AU  - Beletić, Anđelo
AU  - Kovačević-Filipović, Milica
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2132
AB  - Hemolysis and systemic acute inflammation characterize canine babesiosis caused by the intraerythrocytic protozoan parasite Babesia canis. Our hypothesis was that blood redox homeostasis of patients that suffered acute B. canis infection might be disturbed even after treatment with imidocarb-dipropionate and successful clinical recovery. Eight owner dogs with acute B. canis infection were used for this study. We analyzed the complete blood count, acute phase proteins (ceruloplasmin, haptoglobin, paraoxonase-1) in the serum, antioxidant enzymes (catalase and glutathione peroxidase) in the erythrocytes, and oxidative stress markers (malondialdehyde in erythrocytes and thiol groups in serum) at presentation and 15 days after treatment. Results were evaluated by corresponding statistical tests. At presentation, anemia, low/normal leukocyte count and severe thrombocytopenia occurred together with increased ceruloplasmin, haptoglobin levels within the reference interval, decreased paraoxonase-1 and compromised antioxidant defense in the red blood cells. After treatment and successful clinical recovery, hematological values generally fitted within the reference intervals, acute phase proteins were within the physiological levels in the majority of cases and the activities of the antioxidant enzymes were increased. However, elevated malondialdehyde levels indicated increased oxidative damage of erythrocytes that remained as a deleterious sequel despite a successful clinical recovery of the dogs.
AB  - Babezioza pasa, koju izaziva protozoa Babesia canis, praćena je hemolizom i sistemskom akutnom infl amacijom. Naša hipoteza je da kod pasa sa akutnom B. canis infekcijom, čak i nakon terapije imidokarb-dipropionatom i uspešnog kliničkog oporavka, postoji poremećaj redoks homeostaze. Studija uključuje osam vlasničkih pasa akutno infi ciranih babezijom. Analizirani su kompletna krvna slika, proteini akutne faze (ceruloplazmin, haptoglobin i paraoksonaza-1) u serumu, antioksidativni enzimi (katalaza i glutation peroksidaza) u eritrocitima i markeri oksidativnog stresa (malondialdehid u eritrocitima, tiolne grupe u serumu) na prezentaciji i 15 dana nakon terapije. Za procenu značajnosti rezultata primenjeni su odgovarajući statistički testovi. Anemija, nizak/normalan broj leukocita i teška trombocitopenija praćeni povišenom koncentracijom ceruloplazmina, haptoglobinom u fi ziološkim intervalima i sniženim nivoom paraoksonaze-1, kao i kompromitovana antioksidativna zaštita, uočeni su na prezentaciji. Nakon terapije i uspešnog kliničkog oporavka, hematološke vrednosti su bile u fi ziološkom opsegu, kao i proteini akutne faze kod većine pacijenata, a aktivnost antioksidativnih enzima je pojačana. Međutim, povišena koncentracija malondialdehida ukazuje na povećano oštećenje eritrocita koje je zaostalo uprkos uspešnom kliničkom oporavku pasa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs
T1  - Povezanost promena hematoloških parametara, nivoa proteina akutne faze i redoks homeostaze tokom akutne infekcije pasa sa babesia canis
VL  - 71
IS  - 2
SP  - 158
EP  - 169
DO  - 10.2478/acve-2021-0014
ER  - 

@article{
author = "Spariosu, Kristina and Janjić, Filip and Francuski Andrić, Jelena and Radaković, Milena and Beletić, Anđelo and Kovačević-Filipović, Milica and Milanović, Svetlana",
year = "2021",
abstract = "Hemolysis and systemic acute inflammation characterize canine babesiosis caused by the intraerythrocytic protozoan parasite Babesia canis. Our hypothesis was that blood redox homeostasis of patients that suffered acute B. canis infection might be disturbed even after treatment with imidocarb-dipropionate and successful clinical recovery. Eight owner dogs with acute B. canis infection were used for this study. We analyzed the complete blood count, acute phase proteins (ceruloplasmin, haptoglobin, paraoxonase-1) in the serum, antioxidant enzymes (catalase and glutathione peroxidase) in the erythrocytes, and oxidative stress markers (malondialdehyde in erythrocytes and thiol groups in serum) at presentation and 15 days after treatment. Results were evaluated by corresponding statistical tests. At presentation, anemia, low/normal leukocyte count and severe thrombocytopenia occurred together with increased ceruloplasmin, haptoglobin levels within the reference interval, decreased paraoxonase-1 and compromised antioxidant defense in the red blood cells. After treatment and successful clinical recovery, hematological values generally fitted within the reference intervals, acute phase proteins were within the physiological levels in the majority of cases and the activities of the antioxidant enzymes were increased. However, elevated malondialdehyde levels indicated increased oxidative damage of erythrocytes that remained as a deleterious sequel despite a successful clinical recovery of the dogs., Babezioza pasa, koju izaziva protozoa Babesia canis, praćena je hemolizom i sistemskom akutnom infl amacijom. Naša hipoteza je da kod pasa sa akutnom B. canis infekcijom, čak i nakon terapije imidokarb-dipropionatom i uspešnog kliničkog oporavka, postoji poremećaj redoks homeostaze. Studija uključuje osam vlasničkih pasa akutno infi ciranih babezijom. Analizirani su kompletna krvna slika, proteini akutne faze (ceruloplazmin, haptoglobin i paraoksonaza-1) u serumu, antioksidativni enzimi (katalaza i glutation peroksidaza) u eritrocitima i markeri oksidativnog stresa (malondialdehid u eritrocitima, tiolne grupe u serumu) na prezentaciji i 15 dana nakon terapije. Za procenu značajnosti rezultata primenjeni su odgovarajući statistički testovi. Anemija, nizak/normalan broj leukocita i teška trombocitopenija praćeni povišenom koncentracijom ceruloplazmina, haptoglobinom u fi ziološkim intervalima i sniženim nivoom paraoksonaze-1, kao i kompromitovana antioksidativna zaštita, uočeni su na prezentaciji. Nakon terapije i uspešnog kliničkog oporavka, hematološke vrednosti su bile u fi ziološkom opsegu, kao i proteini akutne faze kod većine pacijenata, a aktivnost antioksidativnih enzima je pojačana. Međutim, povišena koncentracija malondialdehida ukazuje na povećano oštećenje eritrocita koje je zaostalo uprkos uspešnom kliničkom oporavku pasa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs, Povezanost promena hematoloških parametara, nivoa proteina akutne faze i redoks homeostaze tokom akutne infekcije pasa sa babesia canis",
volume = "71",
number = "2",
pages = "158-169",
doi = "10.2478/acve-2021-0014"
}

Spariosu, K., Janjić, F., Francuski Andrić, J., Radaković, M., Beletić, A., Kovačević-Filipović, M.,& Milanović, S.. (2021). Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 158-169.
https://doi.org/10.2478/acve-2021-0014

Spariosu K, Janjić F, Francuski Andrić J, Radaković M, Beletić A, Kovačević-Filipović M, Milanović S. Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs. in Acta Veterinaria-Beograd. 2021;71(2):158-169.
doi:10.2478/acve-2021-0014 .

Spariosu, Kristina, Janjić, Filip, Francuski Andrić, Jelena, Radaković, Milena, Beletić, Anđelo, Kovačević-Filipović, Milica, Milanović, Svetlana, "Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs" in Acta Veterinaria-Beograd, 71, no. 2 (2021):158-169,
https://doi.org/10.2478/acve-2021-0014 . .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Došenović, Milan
AU  - Özvegy, József
AU  - Radaković, Milena
AU  - Vejnović, Branislav
AU  - Vučićević, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2215
AB  - Introduction. Glutathione peroxidases are selenoenzymes which have a crucial role in the protection of animals against oxidative stress. 
Materials and Methods. From September 2017 to April 2018, a group of eight red-eared sliders were admitted at the Clinic for Small Animals, Faculty of Veterinary Medicine, University of Belgrade for elective diagnostic celioscopy. The turtles were of unknown age, weight from 1.20 kg to 1.86 kg. The anesthesia protocol involved using ketamine and medetomidine, both at a low dosage (10 mg kg-1 and 0.1 mg kg-1, respectively) as induction, after which anesthesia was maintained using isoflurane at 3% (vapor setting) in 100% oxygen (0.5 L min-1). Medetomidine was reversed with atipamezole (0.2 mg kg-1), given intramuscularly. The elective celioscopy was performed according to standard protocols. One day prior to anesthesia, heparinized blood samples were taken using the subcarapacial venous plexus for venipuncture. The second sampling took place three hours after the anesthetics were administered. 
Results and Conclusions. GPx3 activity in the blood plasmas (n=8) was measured by the coupled test as described by Günzler et al. (1974). Data were tested for normality by the Shapiro-Wilk normality test and the groups were compared using a paired t-test. 
Blood plasma GPx3 activity was significantly higher (p=0.009) after a three-hour recovery period from inhalation anesthesia performed for elective diagnostic celioscopy, than before anesthesia. The measured post-anesthesia GPx3 activities were, on average, 
80% higher than the measurements prior to anesthesia. It can be concluded that the statistically significant increase in the activity of plasma GPx3 from 91.02±36.05 mKat L-1 prior to anesthesia to 160.21±58.94 mKat L-1 three hours after anesthesia is due to the change in oxygen saturation. This is increased to 100% during the procedure, thus exposing the turtles to conditions of high oxygen saturation.
AB  - Uvod. Glutation peroksidaze su selenoenzimi koji igraju ključnu ulogu u zaštiti životinja od oksidativnog stresa. 
Materijal i metode. Od septembra 2017. do aprila 2018. godine, grupa od osam crvenouhih kornjača je primljena na Kliniku za male životinje, Fakulteta Veterinarske Medicine, Univerziteta u Beogradu zbog izvođenja elektivne dijagnostičke celioskopije. Kornjače su bile nepoznate starosti, mase između 1,20 kg i 1,86 kg. Anastetički protokol je uključivao primenu ketamina (10 mg kg-1) i medetomidina (0.1 mg kg-1) za indukciju, oba u niskim dozama, nakon čega je anestezija održavana primenom izoflurana u koncentraciji od 3% (podešen na isparavanje) u 100% kiseoniku (0.5 L min-1). Dejstvo medetomidina je neutralizovano atipamezolom (0.2 mg kg-1), aplikovanim intramuskularno. Elektivna celioskopija je izvedena u skladu sa standardnim protokolima. Heparinizovani uzorci krvi su uzeti jedan dan pre uvođenja u anesteziju venepunkcijom iz subkarapacijalnog venskog pleksusa. Drugo uzorkovanje krvi je uzvršeno tri sata nakon administracije anestetika. 
Rezultati i zaključak. GPx3 aktivnost u krvnoj plazmi (n=8) je izmerena primenom kuplovane reakcije, kao što su opisali Günzler i sar. (1974). Normalna distribucija podataka je testirana pomoću Shapiro - Wilk testa normalnosti (p>0,05), a grupe su poređene koristeći t test za zavisne uzorke. 
Aktivnost GPx3 u krvnoj plazmi je bila značajno viša (p=0.009) u uzorcima uzetim nakon trosatnog perioda oporavka od inhalacione anestezije aplikovane radi izvođenja elektivne celioskopije, nego u uzorcima uzetim pre aplikovanja anestezije. Vrednosti GPx3 izmerene nakon anestezije bile su u proseku 80% više od vrednosti pre anestezije. Može se zaključiti da je statistički značajan porast u aktivnosti GPx3 u plazmi od 91.02±36.05 mKat L-1, pre anestezije do 160.21±58.94 mKat L-1, tri sata nakon anestezije uzrokovan promenom u saturaciji kiseonika. Tokom intervencije saturacija kiseonika se povećava i do 100% čime se kornjače uvode u uslove visoke saturacije kiseonikom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia
T1  - Aktivnost glutation peroksidaze (GPx 3) u plazmi semiakvatičnih kornjača trachemys scripta elegans nakon inhalacione anestezije izofluranom
VL  - 75
IS  - 1
SP  - 76
EP  - 82
DO  - 10.2298/VETGL181208005V
ER  - 

@article{
author = "Valčić, Olivera and Milanović, Svetlana and Došenović, Milan and Özvegy, József and Radaković, Milena and Vejnović, Branislav and Vučićević, Miloš",
year = "2021",
abstract = "Introduction. Glutathione peroxidases are selenoenzymes which have a crucial role in the protection of animals against oxidative stress. 
Materials and Methods. From September 2017 to April 2018, a group of eight red-eared sliders were admitted at the Clinic for Small Animals, Faculty of Veterinary Medicine, University of Belgrade for elective diagnostic celioscopy. The turtles were of unknown age, weight from 1.20 kg to 1.86 kg. The anesthesia protocol involved using ketamine and medetomidine, both at a low dosage (10 mg kg-1 and 0.1 mg kg-1, respectively) as induction, after which anesthesia was maintained using isoflurane at 3% (vapor setting) in 100% oxygen (0.5 L min-1). Medetomidine was reversed with atipamezole (0.2 mg kg-1), given intramuscularly. The elective celioscopy was performed according to standard protocols. One day prior to anesthesia, heparinized blood samples were taken using the subcarapacial venous plexus for venipuncture. The second sampling took place three hours after the anesthetics were administered. 
Results and Conclusions. GPx3 activity in the blood plasmas (n=8) was measured by the coupled test as described by Günzler et al. (1974). Data were tested for normality by the Shapiro-Wilk normality test and the groups were compared using a paired t-test. 
Blood plasma GPx3 activity was significantly higher (p=0.009) after a three-hour recovery period from inhalation anesthesia performed for elective diagnostic celioscopy, than before anesthesia. The measured post-anesthesia GPx3 activities were, on average, 
80% higher than the measurements prior to anesthesia. It can be concluded that the statistically significant increase in the activity of plasma GPx3 from 91.02±36.05 mKat L-1 prior to anesthesia to 160.21±58.94 mKat L-1 three hours after anesthesia is due to the change in oxygen saturation. This is increased to 100% during the procedure, thus exposing the turtles to conditions of high oxygen saturation., Uvod. Glutation peroksidaze su selenoenzimi koji igraju ključnu ulogu u zaštiti životinja od oksidativnog stresa. 
Materijal i metode. Od septembra 2017. do aprila 2018. godine, grupa od osam crvenouhih kornjača je primljena na Kliniku za male životinje, Fakulteta Veterinarske Medicine, Univerziteta u Beogradu zbog izvođenja elektivne dijagnostičke celioskopije. Kornjače su bile nepoznate starosti, mase između 1,20 kg i 1,86 kg. Anastetički protokol je uključivao primenu ketamina (10 mg kg-1) i medetomidina (0.1 mg kg-1) za indukciju, oba u niskim dozama, nakon čega je anestezija održavana primenom izoflurana u koncentraciji od 3% (podešen na isparavanje) u 100% kiseoniku (0.5 L min-1). Dejstvo medetomidina je neutralizovano atipamezolom (0.2 mg kg-1), aplikovanim intramuskularno. Elektivna celioskopija je izvedena u skladu sa standardnim protokolima. Heparinizovani uzorci krvi su uzeti jedan dan pre uvođenja u anesteziju venepunkcijom iz subkarapacijalnog venskog pleksusa. Drugo uzorkovanje krvi je uzvršeno tri sata nakon administracije anestetika. 
Rezultati i zaključak. GPx3 aktivnost u krvnoj plazmi (n=8) je izmerena primenom kuplovane reakcije, kao što su opisali Günzler i sar. (1974). Normalna distribucija podataka je testirana pomoću Shapiro - Wilk testa normalnosti (p>0,05), a grupe su poređene koristeći t test za zavisne uzorke. 
Aktivnost GPx3 u krvnoj plazmi je bila značajno viša (p=0.009) u uzorcima uzetim nakon trosatnog perioda oporavka od inhalacione anestezije aplikovane radi izvođenja elektivne celioskopije, nego u uzorcima uzetim pre aplikovanja anestezije. Vrednosti GPx3 izmerene nakon anestezije bile su u proseku 80% više od vrednosti pre anestezije. Može se zaključiti da je statistički značajan porast u aktivnosti GPx3 u plazmi od 91.02±36.05 mKat L-1, pre anestezije do 160.21±58.94 mKat L-1, tri sata nakon anestezije uzrokovan promenom u saturaciji kiseonika. Tokom intervencije saturacija kiseonika se povećava i do 100% čime se kornjače uvode u uslove visoke saturacije kiseonikom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia, Aktivnost glutation peroksidaze (GPx 3) u plazmi semiakvatičnih kornjača trachemys scripta elegans nakon inhalacione anestezije izofluranom",
volume = "75",
number = "1",
pages = "76-82",
doi = "10.2298/VETGL181208005V"
}

Valčić, O., Milanović, S., Došenović, M., Özvegy, J., Radaković, M., Vejnović, B.,& Vučićević, M.. (2021). Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 76-82.
https://doi.org/10.2298/VETGL181208005V

Valčić O, Milanović S, Došenović M, Özvegy J, Radaković M, Vejnović B, Vučićević M. Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia. in Veterinarski Glasnik. 2021;75(1):76-82.
doi:10.2298/VETGL181208005V .

Valčić, Olivera, Milanović, Svetlana, Došenović, Milan, Özvegy, József, Radaković, Milena, Vejnović, Branislav, Vučićević, Miloš, "Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia" in Veterinarski Glasnik, 75, no. 1 (2021):76-82,
https://doi.org/10.2298/VETGL181208005V . .

TY  - JOUR
AU  - Jovanović, Ljubomir
AU  - Nedić, Sreten
AU  - Bošnjaković, Dušan
AU  - Milanović, Svetlana
AU  - Stojić, Milica
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2273
AB  - Previous studies in the field of acid-base and oxidative status in cows have mainly
focused on the transition period (three weeks before and three weeks after calving).
The aim of this study was to determine the differences in the parameters of acid-
base and oxidative status and electrolyte balance in cows not only during the transition
period, but also at other stages of the production cycle. Holstein-Friesian cows were
divided into four numerically equal groups (n = 6): early lactating cows (9±2 days in
milk - DIM), peak lactating cows (50±5 DIM), late lactating cows (170±10 DIM) and
dry cows (10±1 days before calving). Venous blood samples were taken from the cows
to analyze acid-base status, electrolyte concentrations and oxidative stress parameters,
and to compare group means. Significantly higher pH was observed in early lactating
cows than in late lactating cows. Sodium (Na+) concentration was significantly lower
in early lactating and peak lactating cows compared to dry cows, while chloride
(Cl-) concentration was also lower in late lactating cows compared to dry cows. Plasma
glutathione peroxidase (GSH-Px) activity was higher in early lactating cows compared
to peak lactating cows. Thus, the highest blood pH, lowest Na+ and Cl- concentrations
and highest serum GSH-Px activity were observed in early lactating cows compared to
other groups, indicating the highest metabolic and oxidative stress during this period. In
conclusion, it would be useful to consider the inclusion of these parameters in standard
health assessment procedures in intensive dairy production.
AB  - Prethodna istraživanja na polju acidobaznog i oksidativnog statusa krava uglavnom su
bila usredsređena na tranzicioni period (tri nedelje pre i tri nedelje nakon teljenja). Cilj
ovog istraživanja bio je da se utvrde razlike u parametrima acidobaznog i oksidativnog
statusa, kao i u balansu elektrolita, ne samo u tranzicionom periodu, nego i u ostalim
fazama proizvodnog ciklusa krava. Krave holštajn-frizijske rase podeljene su u četiri
jednake grupe (n=6): krave u ranoj laktaciji (9±2 dana laktacije – DL), krave u piku
laktacije (50±5 DL), krave u kasnoj laktaciji (170±10 DL) i krave u zasušenju (10±1
dana pre očekivanog teljenja). Od ispitivanih krava prikupljeni su uzorci venske krvi
za analizu acidobaznog i oksidativnog statusa i koncentraciju elektrolita. U ovom
istraživanju zabeležena je značajno viša pH vrednost krvi kod krava u ranoj laktaciji
nego kod krava u zasušenju. Koncentracija natrijuma (Na+) bila je značajno niža kod
krava u ranoj laktaciji i piku laktacije u poređenju sa kravama u zasušenju, dok je
koncentracija hlora (Cl-
) bila značajno niža i kod krava u kasnoj laktaciji u poređenju
sa kravama u zasušenju. Aktivnost glutation peroksidaze (GSH-Px) bila je značajno
viša kod krava u ranoj laktaciji u poređenju sa kravama u piku laktacije. Najviše pH
vrednosti krvi, najniža koncentracija Na+ i Cl-
 i najviša aktivnost GSH-Px zabeleženi su
kod krava u ranoj laktaciji, što ukazuje na najveći metabolički i oksidativni stres tokom
ovog perioda. Imajući u vidu dobijene razlike bilo bi značajno razmotriti uvođenje parametara ispitivanih u ovom radu u standardne procedure za procenu zdravlja krava
u intenzivnoj proizvodnji mleka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle
T1  - Acidobazni status, balans elektrolita i oksidativni status kod visokomlečnih krava u različitim fazama proizvodnog ciklusa
VL  - 00
SP  - 1
EP  - 12
DO  - 10.2298/VETGL210130004J
ER  - 

@article{
author = "Jovanović, Ljubomir and Nedić, Sreten and Bošnjaković, Dušan and Milanović, Svetlana and Stojić, Milica and Vujanac, Ivan and Prodanović, Radiša and Kirovski, Danijela",
year = "2021",
abstract = "Previous studies in the field of acid-base and oxidative status in cows have mainly
focused on the transition period (three weeks before and three weeks after calving).
The aim of this study was to determine the differences in the parameters of acid-
base and oxidative status and electrolyte balance in cows not only during the transition
period, but also at other stages of the production cycle. Holstein-Friesian cows were
divided into four numerically equal groups (n = 6): early lactating cows (9±2 days in
milk - DIM), peak lactating cows (50±5 DIM), late lactating cows (170±10 DIM) and
dry cows (10±1 days before calving). Venous blood samples were taken from the cows
to analyze acid-base status, electrolyte concentrations and oxidative stress parameters,
and to compare group means. Significantly higher pH was observed in early lactating
cows than in late lactating cows. Sodium (Na+) concentration was significantly lower
in early lactating and peak lactating cows compared to dry cows, while chloride
(Cl-) concentration was also lower in late lactating cows compared to dry cows. Plasma
glutathione peroxidase (GSH-Px) activity was higher in early lactating cows compared
to peak lactating cows. Thus, the highest blood pH, lowest Na+ and Cl- concentrations
and highest serum GSH-Px activity were observed in early lactating cows compared to
other groups, indicating the highest metabolic and oxidative stress during this period. In
conclusion, it would be useful to consider the inclusion of these parameters in standard
health assessment procedures in intensive dairy production., Prethodna istraživanja na polju acidobaznog i oksidativnog statusa krava uglavnom su
bila usredsređena na tranzicioni period (tri nedelje pre i tri nedelje nakon teljenja). Cilj
ovog istraživanja bio je da se utvrde razlike u parametrima acidobaznog i oksidativnog
statusa, kao i u balansu elektrolita, ne samo u tranzicionom periodu, nego i u ostalim
fazama proizvodnog ciklusa krava. Krave holštajn-frizijske rase podeljene su u četiri
jednake grupe (n=6): krave u ranoj laktaciji (9±2 dana laktacije – DL), krave u piku
laktacije (50±5 DL), krave u kasnoj laktaciji (170±10 DL) i krave u zasušenju (10±1
dana pre očekivanog teljenja). Od ispitivanih krava prikupljeni su uzorci venske krvi
za analizu acidobaznog i oksidativnog statusa i koncentraciju elektrolita. U ovom
istraživanju zabeležena je značajno viša pH vrednost krvi kod krava u ranoj laktaciji
nego kod krava u zasušenju. Koncentracija natrijuma (Na+) bila je značajno niža kod
krava u ranoj laktaciji i piku laktacije u poređenju sa kravama u zasušenju, dok je
koncentracija hlora (Cl-
) bila značajno niža i kod krava u kasnoj laktaciji u poređenju
sa kravama u zasušenju. Aktivnost glutation peroksidaze (GSH-Px) bila je značajno
viša kod krava u ranoj laktaciji u poređenju sa kravama u piku laktacije. Najviše pH
vrednosti krvi, najniža koncentracija Na+ i Cl-
 i najviša aktivnost GSH-Px zabeleženi su
kod krava u ranoj laktaciji, što ukazuje na najveći metabolički i oksidativni stres tokom
ovog perioda. Imajući u vidu dobijene razlike bilo bi značajno razmotriti uvođenje parametara ispitivanih u ovom radu u standardne procedure za procenu zdravlja krava
u intenzivnoj proizvodnji mleka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle, Acidobazni status, balans elektrolita i oksidativni status kod visokomlečnih krava u različitim fazama proizvodnog ciklusa",
volume = "00",
pages = "1-12",
doi = "10.2298/VETGL210130004J"
}

Jovanović, L., Nedić, S., Bošnjaković, D., Milanović, S., Stojić, M., Vujanac, I., Prodanović, R.,& Kirovski, D.. (2021). Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 00, 1-12.
https://doi.org/10.2298/VETGL210130004J

Jovanović L, Nedić S, Bošnjaković D, Milanović S, Stojić M, Vujanac I, Prodanović R, Kirovski D. Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle. in Veterinarski Glasnik. 2021;00:1-12.
doi:10.2298/VETGL210130004J .

Jovanović, Ljubomir, Nedić, Sreten, Bošnjaković, Dušan, Milanović, Svetlana, Stojić, Milica, Vujanac, Ivan, Prodanović, Radiša, Kirovski, Danijela, "Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle" in Veterinarski Glasnik, 00 (2021):1-12,
https://doi.org/10.2298/VETGL210130004J . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3202
AB  - Mleko magarice se od davnina koristi u medicinske i kozmetičke svrhe, međutim
malo je bilo poznato koji molekuli su odgovorni za njegove protivupalne i
antimikrobne efekte. Poslednjih decenija, interesovanje naučnika za magareće
mleko je poraslo što je rezultiralo boljim poznavanjam njegovog sastava i
karakteristika. Po svom sastavu, magareće mleko je blisko mleku žene, prvenstveno
zbog niskog sadržaja masti i kazeina. Istovremeno, zahvaljujući karakteristikama
proteinskih frakcija, ono je pogodno za primenu kod osoba koje su
alergične na kravlje mleko. Ustanovljeno je prisustvo niza molekula, posebno
proteina i peptida, koji imaju anti-inflamatorno i antimikrobno dejstvo. Među
njima se posebno ističu lizozim, laktoferin, i laktoperoksidaza. U odnosu na humano
i kravlje mleko, mleko magarice je posebno bogato lizozimom. U ovom
radu su detaljnije opisane karakteristike i mehanizam delovanja navedenih
proteina.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
T1  - Antiinflamatorni i antimikrobni efekti magarećeg mleka
SP  - 431
EP  - 439
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3202
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2021",
abstract = "Mleko magarice se od davnina koristi u medicinske i kozmetičke svrhe, međutim
malo je bilo poznato koji molekuli su odgovorni za njegove protivupalne i
antimikrobne efekte. Poslednjih decenija, interesovanje naučnika za magareće
mleko je poraslo što je rezultiralo boljim poznavanjam njegovog sastava i
karakteristika. Po svom sastavu, magareće mleko je blisko mleku žene, prvenstveno
zbog niskog sadržaja masti i kazeina. Istovremeno, zahvaljujući karakteristikama
proteinskih frakcija, ono je pogodno za primenu kod osoba koje su
alergične na kravlje mleko. Ustanovljeno je prisustvo niza molekula, posebno
proteina i peptida, koji imaju anti-inflamatorno i antimikrobno dejstvo. Među
njima se posebno ističu lizozim, laktoferin, i laktoperoksidaza. U odnosu na humano
i kravlje mleko, mleko magarice je posebno bogato lizozimom. U ovom
radu su detaljnije opisane karakteristike i mehanizam delovanja navedenih
proteina.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021",
title = "Antiinflamatorni i antimikrobni efekti magarećeg mleka",
pages = "431-439",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3202"
}

Valčić, O.,& Milanović, S.. (2021). Antiinflamatorni i antimikrobni efekti magarećeg mleka. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
Beograd : Srpsko veterinarsko društvo., 431-439.
https://hdl.handle.net/21.15107/rcub_veterinar_3202

Valčić O, Milanović S. Antiinflamatorni i antimikrobni efekti magarećeg mleka. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021. 2021;:431-439.
https://hdl.handle.net/21.15107/rcub_veterinar_3202 .

Valčić, Olivera, Milanović, Svetlana, "Antiinflamatorni i antimikrobni efekti magarećeg mleka" in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021 (2021):431-439,
https://hdl.handle.net/21.15107/rcub_veterinar_3202 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3410
AB  - Заједно са селеном, цинком, бакром, јодом и др. молибден спада у есенцијалне
микроелементе који су неопходни за нормално одвијање метаболичких
процеса. У природи се молибден не налази у елементарном стању већ је
првенствено присутан у облику молибдената (MoS2) и феримолибдата
(Fe2(MoO4)2), док је у мешаоницама сточне хране чест суплемент у облику
натријум молибдата. У природи добар извор молибдена за домаће животиње
представљају биљна хранива (цереалије) и лиснато поврће у којима је
просечан садржај молибдена од 0,5 до 100 мг/кг СМ. Забележена су тровања
животиња која су на испаши где су присутне биљке са високим садржајем овог
микроелемента. Добро се ресорбује у танким цревима, док је у ткивима
присутан првенствено у јетри, бубрезима и панкреасу. Полуживот у
организму је кратак, износи свега неколико часова и излучује се преко урина.
Код животиња, молибден улази у састав четири различита ензима
(металофлавопротеина): ксантин оксидазе, алдехид оксидазе, сулфит
оксидазе и митохондријална амидоксин редуктаза (мАМЦ). У наведеним
ензимима молибден је присутан у облику комплекса молибдоптерина преко
којег учествује у реакцијама хидроксилације.
Клиничка слика дефицита молибдена забележена је код коза, јагњади, и
живине. Појава патологије везане за дефицит није увек последица недовољног
уноса, већ може бити и последица генетски контролисане неадекватне синтезе
и уградње молибдоптерина. Значајно већи и чешћи проблем представља
питање токсичности молибдена. Краве и овце су осетљиве на дозе до 30 мг/кг,
док коњи, свиње и козе толеришу дозе до 1000 мг/кг. Молибденоза код говеда
настаје након уноса високих доза молибдена, уз настанак тритиомолибдата, и
уз истовремено ниске дозе бакра. Симптоми укључују анемију,
гастроинтестиналне сметње, смањен раст и отежану репродукцију. Код телади
забележена је перидонтална инфламација и губитак зуба.
Забележене су сложене интеракције између молибдена, бакра и сумпора.
Ефекти молибдена се могу објаснити променама афинитета лиганда за бакар,
услед чега долази до поремећаја дистрибуције бакра и његовог метаболичког
дефицита. Молибден је антидот у случају тровања бакром, међутим
механизам деловања још увек није у потпуности расветљен.
AB  - Together with selenium, zinc, copper, iodine, etc. molybdenum is an essential
microelement required for metabolic processes in the cell. In nature molybdenum
is not present in its pure elementary form, but as molybdenate (MoS2) and
ferrimoyibdate (Fe2(MoO4)2), while in the feedstuff industry is a common
supplement in the form of sodium molybdate. A good source of molybdenum for
animals are plant feedstuffs (cereals) and leafy vegetables. In these plants the
average molybdenum concentration ranges from 0.5 to 100 mg/kg DM. Cases of
poisoning have been reported in situations where the animals were on pasture
grazing on plants with a high content of this microelement. It is well absorbed in
the small intestine, and is mainly present in the organism in the liver, kidneys and
pancreas. Its half-life in the organism is short, only a few hours, and is excreted by
means of urine.
In the animal kingdom molybdenum is a component of four different enzymes
(metaloflavoproteins): xanthine oxidase, aldehyde oxidase, sulphite oxidase, and
mitochondrial amidoxin reductase (mAMC). In these enzymes molybdenum is
present as a molybdopterine complex by means of which takes part in
hydroxylation reactions.
Clinically manifested molybdenum deficiency has been recorded in goats, lambs,
and poultry. The existence of pathological signs is not always the result of an
inadequate intake, as it can be the consequence of genetically controlled
inadequate synthesis and incorporation of molybdopteridine. A significantly
more evident problem is presented as the result of molybdenum toxicity. Cattle
and sheep are sensitive to doses of about 30 mg/kg, but horses, pigs and goats can
tolerate up to 1000 mg/kg. Molybdenosis in cattle due to high doses of
molybdenum develops as tritiomolibdate forms concurrent to low copper doses.
The symptoms include anemia, gastrointestinal disorders, stunt growth and poor
reproduction. Calves may develop periodontal inflammation and teeth loss.
Complex interactions between molybdenum, copper and sulphur are
documented. The effects of molybdenum can be explained by changes of the
affinity of copper ligands which results in changes of copper distribution and
metabolic deficiency. Molibdenum is an effective antidote for copper poisoning,
however the mechanism of action is still not elucidated.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021
T1  - Zaboravljeni esencijalni mikroelement- molibden
T1  - The forgotten essential microelement- molybdenum
SP  - 175
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3410
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2021",
abstract = "Заједно са селеном, цинком, бакром, јодом и др. молибден спада у есенцијалне
микроелементе који су неопходни за нормално одвијање метаболичких
процеса. У природи се молибден не налази у елементарном стању већ је
првенствено присутан у облику молибдената (MoS2) и феримолибдата
(Fe2(MoO4)2), док је у мешаоницама сточне хране чест суплемент у облику
натријум молибдата. У природи добар извор молибдена за домаће животиње
представљају биљна хранива (цереалије) и лиснато поврће у којима је
просечан садржај молибдена од 0,5 до 100 мг/кг СМ. Забележена су тровања
животиња која су на испаши где су присутне биљке са високим садржајем овог
микроелемента. Добро се ресорбује у танким цревима, док је у ткивима
присутан првенствено у јетри, бубрезима и панкреасу. Полуживот у
организму је кратак, износи свега неколико часова и излучује се преко урина.
Код животиња, молибден улази у састав четири различита ензима
(металофлавопротеина): ксантин оксидазе, алдехид оксидазе, сулфит
оксидазе и митохондријална амидоксин редуктаза (мАМЦ). У наведеним
ензимима молибден је присутан у облику комплекса молибдоптерина преко
којег учествује у реакцијама хидроксилације.
Клиничка слика дефицита молибдена забележена је код коза, јагњади, и
живине. Појава патологије везане за дефицит није увек последица недовољног
уноса, већ може бити и последица генетски контролисане неадекватне синтезе
и уградње молибдоптерина. Значајно већи и чешћи проблем представља
питање токсичности молибдена. Краве и овце су осетљиве на дозе до 30 мг/кг,
док коњи, свиње и козе толеришу дозе до 1000 мг/кг. Молибденоза код говеда
настаје након уноса високих доза молибдена, уз настанак тритиомолибдата, и
уз истовремено ниске дозе бакра. Симптоми укључују анемију,
гастроинтестиналне сметње, смањен раст и отежану репродукцију. Код телади
забележена је перидонтална инфламација и губитак зуба.
Забележене су сложене интеракције између молибдена, бакра и сумпора.
Ефекти молибдена се могу објаснити променама афинитета лиганда за бакар,
услед чега долази до поремећаја дистрибуције бакра и његовог метаболичког
дефицита. Молибден је антидот у случају тровања бакром, међутим
механизам деловања још увек није у потпуности расветљен., Together with selenium, zinc, copper, iodine, etc. molybdenum is an essential
microelement required for metabolic processes in the cell. In nature molybdenum
is not present in its pure elementary form, but as molybdenate (MoS2) and
ferrimoyibdate (Fe2(MoO4)2), while in the feedstuff industry is a common
supplement in the form of sodium molybdate. A good source of molybdenum for
animals are plant feedstuffs (cereals) and leafy vegetables. In these plants the
average molybdenum concentration ranges from 0.5 to 100 mg/kg DM. Cases of
poisoning have been reported in situations where the animals were on pasture
grazing on plants with a high content of this microelement. It is well absorbed in
the small intestine, and is mainly present in the organism in the liver, kidneys and
pancreas. Its half-life in the organism is short, only a few hours, and is excreted by
means of urine.
In the animal kingdom molybdenum is a component of four different enzymes
(metaloflavoproteins): xanthine oxidase, aldehyde oxidase, sulphite oxidase, and
mitochondrial amidoxin reductase (mAMC). In these enzymes molybdenum is
present as a molybdopterine complex by means of which takes part in
hydroxylation reactions.
Clinically manifested molybdenum deficiency has been recorded in goats, lambs,
and poultry. The existence of pathological signs is not always the result of an
inadequate intake, as it can be the consequence of genetically controlled
inadequate synthesis and incorporation of molybdopteridine. A significantly
more evident problem is presented as the result of molybdenum toxicity. Cattle
and sheep are sensitive to doses of about 30 mg/kg, but horses, pigs and goats can
tolerate up to 1000 mg/kg. Molybdenosis in cattle due to high doses of
molybdenum develops as tritiomolibdate forms concurrent to low copper doses.
The symptoms include anemia, gastrointestinal disorders, stunt growth and poor
reproduction. Calves may develop periodontal inflammation and teeth loss.
Complex interactions between molybdenum, copper and sulphur are
documented. The effects of molybdenum can be explained by changes of the
affinity of copper ligands which results in changes of copper distribution and
metabolic deficiency. Molibdenum is an effective antidote for copper poisoning,
however the mechanism of action is still not elucidated.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021",
title = "Zaboravljeni esencijalni mikroelement- molibden, The forgotten essential microelement- molybdenum",
pages = "175-176",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3410"
}

Valčić, O.,& Milanović, S.. (2021). Zaboravljeni esencijalni mikroelement- molibden. in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021
Banja Luka : Veterinarska komora Republike Srpske., 175-176.
https://hdl.handle.net/21.15107/rcub_veterinar_3410

Valčić O, Milanović S. Zaboravljeni esencijalni mikroelement- molibden. in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021. 2021;:175-176.
https://hdl.handle.net/21.15107/rcub_veterinar_3410 .

Valčić, Olivera, Milanović, Svetlana, "Zaboravljeni esencijalni mikroelement- molibden" in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021 (2021):175-176,
https://hdl.handle.net/21.15107/rcub_veterinar_3410 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3577
AB  - Intenzivna živinarska proizvodnja nosi sa sobom puno izazova, jer je potrebno
za što kraće vreme proizvesti kvalitetan i zdravstveno ispravan proizvod. Kako
bi se maksimalno iskoristio genetski potencijal jedinki, nameće se upotreba
kvalitetnih hraniva koja će u potpunosti zadovoljiti njihove osnovne i proizvodne potrebe. Poseban izazov predstavlja način držanja živine u intenzivnoj proizvodnji koji pogoduje brzom širenju zaraznih bolesti. Zbog svega navedenog,
hrana za živinu sadrži mnoge dodatke koji će poboljšati proizvodne karakteristike, a takođe, uticati na uspešniji razvoj imuniteta nakon vakcinacije i odbranu organizma u slučaju pojave infekcije.
U intenzivnoj proizvodnji dodavanje mikroelemenata predstavlja jedan od
ključnih faktora potrebnih za funkcionisanje organizma. U ovom radu će fokus
biti na njihovom uticaju na imunitet.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXIX Savetovanje živinara, online, 5. 11. 2020
T1  - Uloga i značaj mikroelemenata za imunitet živine
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3577
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2021",
abstract = "Intenzivna živinarska proizvodnja nosi sa sobom puno izazova, jer je potrebno
za što kraće vreme proizvesti kvalitetan i zdravstveno ispravan proizvod. Kako
bi se maksimalno iskoristio genetski potencijal jedinki, nameće se upotreba
kvalitetnih hraniva koja će u potpunosti zadovoljiti njihove osnovne i proizvodne potrebe. Poseban izazov predstavlja način držanja živine u intenzivnoj proizvodnji koji pogoduje brzom širenju zaraznih bolesti. Zbog svega navedenog,
hrana za živinu sadrži mnoge dodatke koji će poboljšati proizvodne karakteristike, a takođe, uticati na uspešniji razvoj imuniteta nakon vakcinacije i odbranu organizma u slučaju pojave infekcije.
U intenzivnoj proizvodnji dodavanje mikroelemenata predstavlja jedan od
ključnih faktora potrebnih za funkcionisanje organizma. U ovom radu će fokus
biti na njihovom uticaju na imunitet.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXIX Savetovanje živinara, online, 5. 11. 2020",
title = "Uloga i značaj mikroelemenata za imunitet živine",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3577"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2021). Uloga i značaj mikroelemenata za imunitet živine. in XXIX Savetovanje živinara, online, 5. 11. 2020
Beograd : Ciiip Živinarstvo..
https://hdl.handle.net/21.15107/rcub_veterinar_3577

Milanović S, Valčić O, Jovanović I. Uloga i značaj mikroelemenata za imunitet živine. in XXIX Savetovanje živinara, online, 5. 11. 2020. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3577 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Uloga i značaj mikroelemenata za imunitet živine" in XXIX Savetovanje živinara, online, 5. 11. 2020 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3577 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3578
AB  - Selen je mikroelement koji ulazi u sastav mnogih proteina i enzima neophodnih
u odbrani od oksidativnog stresa, aktivaciji i deaktivaciji tireoidnih
hormona i adekvatnom imunskom odgovoru. Poseban izazov u intenzivnoj
živinarskoj proizvodnji predstavlja način držanja ptica, koji pogoduje širenju
zaraznih bolesti, te je pravilna i balansirana ishrana, osim za zadovoljenje
osnovnih i proizvodnih potreba jedinki, neophodna i za pravilan rad imunskog
sistema. Selen je, pored ostalih mikroelemenata, izuzetno važan u tom smislu.
On kao gradivni element proteina i enzima utiče na gotovo sve segmente imunskog
sistema (urođeni i stečeni, celularni i humoralni).
Ova činjenica je važna jer dodavanje u hranu selena u obliku selenocisteina i
selenometionina može imati različit efekat zbog toga što se metabolizam ovih
aminokiselina i neorganskog selena vrlo razlikuju.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021
T1  - Uticaj selena na imunski sistem živine
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3578
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2021",
abstract = "Selen je mikroelement koji ulazi u sastav mnogih proteina i enzima neophodnih
u odbrani od oksidativnog stresa, aktivaciji i deaktivaciji tireoidnih
hormona i adekvatnom imunskom odgovoru. Poseban izazov u intenzivnoj
živinarskoj proizvodnji predstavlja način držanja ptica, koji pogoduje širenju
zaraznih bolesti, te je pravilna i balansirana ishrana, osim za zadovoljenje
osnovnih i proizvodnih potreba jedinki, neophodna i za pravilan rad imunskog
sistema. Selen je, pored ostalih mikroelemenata, izuzetno važan u tom smislu.
On kao gradivni element proteina i enzima utiče na gotovo sve segmente imunskog
sistema (urođeni i stečeni, celularni i humoralni).
Ova činjenica je važna jer dodavanje u hranu selena u obliku selenocisteina i
selenometionina može imati različit efekat zbog toga što se metabolizam ovih
aminokiselina i neorganskog selena vrlo razlikuju.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021",
title = "Uticaj selena na imunski sistem živine",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3578"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2021). Uticaj selena na imunski sistem živine. in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021
Beograd : Ciiip Živinarstvo..
https://hdl.handle.net/21.15107/rcub_veterinar_3578

Milanović S, Valčić O, Jovanović I. Uticaj selena na imunski sistem živine. in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3578 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Uticaj selena na imunski sistem živine" in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3578 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3371
AB  - Zahtevi za sve većim proizvodnim rezultatima, intenzivna selekcija, kao i globalno
otopljavanje doveli su do pojave toplotnog stresa. Predhodna saznanja su tumačila
nastali pad proizvodnih rezultata isključivo kao posledica inapetence usled
visokih temperatura. Međutim, toplotni stres dovodi do niza poremećaja na nivou
fiziološke homeostaze i metaboličkih procesa. Selekcija u cilju veće proizvodnje
mišićne mase je rezultirala povećanom proizvodnjom metaboličke toplote, usled
čega dolazi do hiperventilacije i posledične metaboličke acidoze što dodatno
opterećuje organizam. Zbog nastalog toplotnog stresa, uprkos smanjenom unosu
hrane, dolazi do smanjenog lipolitičkog kapaciteta masnog tkiva i smanjene
koncentracije ne-esterifikovanih masnih kiselina (NEFA) u krvnoj plazmi goveda i
svinja. Kao odgovor na ćelijski stres dolazi do porasta koncentracije insulina i
povećanih potreba za glukozom radi očuvanja normoglikemije. Istovremeno,
značajno opada aktivnost tireoidnih hormona T3 i T4. Preraspodela cirkulacije
koja nastaje usled toplotnog stresa dovodi do mobilizacije krvi iz digestivnog
trakta što dovodi do hipoksije creva usled koje se javlja poremećaj propustljivosti
sluzokože i prolaska lipopolisaharida bakterija u portalni i sistemski krvotok.
Paralelno sa navedenim procesima dolazi do intenzivne glikogenolize u mišićima
usled aktivacije glikogen fosforilaze. Toplotni stres je jedan od pokretača procesa
nastanka slobodnih kiseoničnih radikala, oksidativnog stresa i sinteze
malonildialdehida koji dodatno oštećuju integritet ćelijskih membrana.
Biohemijski profil krvi goveda, svinja i živine ukazuje na porast koncentracije
proteina toplotnog šoka (HSP), proizvoda peroksidacije masnih kiselina (MDA),
enzima zaštite od oksidativnog stresa (SOD, GPx), kao i hemoglobina.
Navedeni poremećaji dovode do promene kvaliteta goveđeg (DFD- dry firm dark
meat) i svinjskog mesa (PSE- pale soft exudative) što rezultira značajnim
ekonomskim gubitcima u proizvodnji i plasmanu.
Usled navedenog, neophodno je dublje sagledati problematiku toplotnog stresa u
uslovima intenzivne proizvodnje kako bi se negativni efekti maksimalno sprečili
ili ublažili.
AB  - Demands for increasing production results, as well as global warming have led to
the emergence of heat stress. Previous knowledge interpreted the resulting
decline in production solely as a consequence of a decreased appetite. However,
heat stress leads to a number of disorders at the level of physiological homeostasis
and metabolic processes. Selection for higher muscle mass resulted in increased
metabolic heat production. Due to the increase in heat, hyperventilation and
consequent metabolic acidosis occur, which additionally burdens the organism.
Due to the resulting heat stress, despite the reduced food intake, there is a reduced
lipolytic capacity of adipose tissue and a reduced concentration of non-esterified
fatty acids (NEFA) in the blood plasma of cattle and pigs. In response to cellular
stress, insulin concentrations increase and glucose needs increase in order to
preserve normoglycemia. At the same time, the activity of thyroid hormones T3
and T4 decreases significantly. The redistribution of circulation caused by heat
stress leads to the mobilization of blood from the digestive tract, which results in
intestinal hypoxia, which results in increased permeability of the mucous
membrane and the passage of bacterial lipopolysaccharides into the portal and
systemic bloodstream. In parallel with the mentioned processes, intensive
glycogenolysis occurs in the muscles due to the activation of glycogen
phosphorylase. Heat stress is one of the initiators of the process of formation of
free oxygen radicals, oxidative stress and synthesis of malonyldialdehyde, which
additionally damage the integrity of cell membranes. The biochemical profile of
the blood of cattle, pigs and poultry indicates an increase in the concentration of
heat shock protein (HSP), fatty acid peroxidation products (MDA), enzymes
involved in the protection against oxidative stress (SOD, GPx), as well as
hemoglobin.
These disorders lead to changes in the quality of beef (DFD - dry firm dark meat)
and pork (PSE - pale soft exudative), which results in significant economic losses
in production and marketing.
Due to the above, it is necessary to look deeper into the issue of heat stress in
conditions of intensive production in order to maximally prevent or mitigate the
negative effects.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
T1  - Efekti toplotnog stresa na metabolizam farmskih životinja
T1  - Effects of heat stress on metabolism in farm animals
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3371
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2020",
abstract = "Zahtevi za sve većim proizvodnim rezultatima, intenzivna selekcija, kao i globalno
otopljavanje doveli su do pojave toplotnog stresa. Predhodna saznanja su tumačila
nastali pad proizvodnih rezultata isključivo kao posledica inapetence usled
visokih temperatura. Međutim, toplotni stres dovodi do niza poremećaja na nivou
fiziološke homeostaze i metaboličkih procesa. Selekcija u cilju veće proizvodnje
mišićne mase je rezultirala povećanom proizvodnjom metaboličke toplote, usled
čega dolazi do hiperventilacije i posledične metaboličke acidoze što dodatno
opterećuje organizam. Zbog nastalog toplotnog stresa, uprkos smanjenom unosu
hrane, dolazi do smanjenog lipolitičkog kapaciteta masnog tkiva i smanjene
koncentracije ne-esterifikovanih masnih kiselina (NEFA) u krvnoj plazmi goveda i
svinja. Kao odgovor na ćelijski stres dolazi do porasta koncentracije insulina i
povećanih potreba za glukozom radi očuvanja normoglikemije. Istovremeno,
značajno opada aktivnost tireoidnih hormona T3 i T4. Preraspodela cirkulacije
koja nastaje usled toplotnog stresa dovodi do mobilizacije krvi iz digestivnog
trakta što dovodi do hipoksije creva usled koje se javlja poremećaj propustljivosti
sluzokože i prolaska lipopolisaharida bakterija u portalni i sistemski krvotok.
Paralelno sa navedenim procesima dolazi do intenzivne glikogenolize u mišićima
usled aktivacije glikogen fosforilaze. Toplotni stres je jedan od pokretača procesa
nastanka slobodnih kiseoničnih radikala, oksidativnog stresa i sinteze
malonildialdehida koji dodatno oštećuju integritet ćelijskih membrana.
Biohemijski profil krvi goveda, svinja i živine ukazuje na porast koncentracije
proteina toplotnog šoka (HSP), proizvoda peroksidacije masnih kiselina (MDA),
enzima zaštite od oksidativnog stresa (SOD, GPx), kao i hemoglobina.
Navedeni poremećaji dovode do promene kvaliteta goveđeg (DFD- dry firm dark
meat) i svinjskog mesa (PSE- pale soft exudative) što rezultira značajnim
ekonomskim gubitcima u proizvodnji i plasmanu.
Usled navedenog, neophodno je dublje sagledati problematiku toplotnog stresa u
uslovima intenzivne proizvodnje kako bi se negativni efekti maksimalno sprečili
ili ublažili., Demands for increasing production results, as well as global warming have led to
the emergence of heat stress. Previous knowledge interpreted the resulting
decline in production solely as a consequence of a decreased appetite. However,
heat stress leads to a number of disorders at the level of physiological homeostasis
and metabolic processes. Selection for higher muscle mass resulted in increased
metabolic heat production. Due to the increase in heat, hyperventilation and
consequent metabolic acidosis occur, which additionally burdens the organism.
Due to the resulting heat stress, despite the reduced food intake, there is a reduced
lipolytic capacity of adipose tissue and a reduced concentration of non-esterified
fatty acids (NEFA) in the blood plasma of cattle and pigs. In response to cellular
stress, insulin concentrations increase and glucose needs increase in order to
preserve normoglycemia. At the same time, the activity of thyroid hormones T3
and T4 decreases significantly. The redistribution of circulation caused by heat
stress leads to the mobilization of blood from the digestive tract, which results in
intestinal hypoxia, which results in increased permeability of the mucous
membrane and the passage of bacterial lipopolysaccharides into the portal and
systemic bloodstream. In parallel with the mentioned processes, intensive
glycogenolysis occurs in the muscles due to the activation of glycogen
phosphorylase. Heat stress is one of the initiators of the process of formation of
free oxygen radicals, oxidative stress and synthesis of malonyldialdehyde, which
additionally damage the integrity of cell membranes. The biochemical profile of
the blood of cattle, pigs and poultry indicates an increase in the concentration of
heat shock protein (HSP), fatty acid peroxidation products (MDA), enzymes
involved in the protection against oxidative stress (SOD, GPx), as well as
hemoglobin.
These disorders lead to changes in the quality of beef (DFD - dry firm dark meat)
and pork (PSE - pale soft exudative), which results in significant economic losses
in production and marketing.
Due to the above, it is necessary to look deeper into the issue of heat stress in
conditions of intensive production in order to maximally prevent or mitigate the
negative effects.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020",
title = "Efekti toplotnog stresa na metabolizam farmskih životinja, Effects of heat stress on metabolism in farm animals",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3371"
}

Valčić, O.,& Milanović, S.. (2020). Efekti toplotnog stresa na metabolizam farmskih životinja. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
Banja Luka : Veterinarska komora Republike Srpske., 152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_3371

Valčić O, Milanović S. Efekti toplotnog stresa na metabolizam farmskih životinja. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020. 2020;:152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_3371 .

Valčić, Olivera, Milanović, Svetlana, "Efekti toplotnog stresa na metabolizam farmskih životinja" in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020 (2020):152-153,
https://hdl.handle.net/21.15107/rcub_veterinar_3371 .

TY  - JOUR
AU  - Kosanović, Maja
AU  - Cvetković, Jelena
AU  - Gruden-Movsesijan, Alisa
AU  - Vasilev, Saša
AU  - Milanović, Svetlana
AU  - Ilić, Natasa
AU  - Sofronic-Milosavljević, Ljiljana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1785
AB  - Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.
PB  - Wiley, Hoboken
T2  - Parasite Immunology
T1  - Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties
VL  - 41
IS  - 10
SP  - e12665
DO  - 10.1111/pim.12665
ER  - 

@article{
author = "Kosanović, Maja and Cvetković, Jelena and Gruden-Movsesijan, Alisa and Vasilev, Saša and Milanović, Svetlana and Ilić, Natasa and Sofronic-Milosavljević, Ljiljana",
year = "2019",
abstract = "Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.",
publisher = "Wiley, Hoboken",
journal = "Parasite Immunology",
title = "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties",
volume = "41",
number = "10",
pages = "e12665",
doi = "10.1111/pim.12665"
}

Kosanović, M., Cvetković, J., Gruden-Movsesijan, A., Vasilev, S., Milanović, S., Ilić, N.,& Sofronic-Milosavljević, L.. (2019). Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology
Wiley, Hoboken., 41(10), e12665.
https://doi.org/10.1111/pim.12665

Kosanović M, Cvetković J, Gruden-Movsesijan A, Vasilev S, Milanović S, Ilić N, Sofronic-Milosavljević L. Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology. 2019;41(10):e12665.
doi:10.1111/pim.12665 .

Kosanović, Maja, Cvetković, Jelena, Gruden-Movsesijan, Alisa, Vasilev, Saša, Milanović, Svetlana, Ilić, Natasa, Sofronic-Milosavljević, Ljiljana, "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties" in Parasite Immunology, 41, no. 10 (2019):e12665,
https://doi.org/10.1111/pim.12665 . .

TY  - JOUR
AU  - Nikšić, Aleksandar
AU  - Ječmenica, Jovana
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2181
AB  - Cilj ovog rada je bio da se utvrdi da li postoje promene u koncentraciji glukoze, beta-hidroksibutirata (BHB) i aktivnosti glutation-peroksidaze (GPx) u zavisnosti od telesne kondicije kod junica. Junice su na osnovu telesne kondicije (TK) svrstane u dve grupe: 1. TK=3,75 i 2. TK ≥4,0. U ogledu je korišćena puna krv 23 junice uzeta 1-10 dan nakon partusa. Koncentracije glukoze i BHB određivane su odmah po uzorkovanju krvi, a aktivnost GPX određivana je nakon 24 sata. Junice sa većom telesnom kondicijom imale su statistički značajno veću prosečnu koncentraciju BHB i aktivnost GPX. Prosečna koncentracija glukoze u obe grupe se nije razlikovala.
AB  - The aim of this study was to establish if there are differences in glucose and ß-hydroxybutyrate (BHB) concentrations as well as glutathione peroxidase (GPx) activity related to the body condition in heifers. Based on the body condition (BC), heifers were divided into two groups: 1. BC=3.75 and 2. BC≥4.0. Whole blood samples from 23 heifers, taken between day 1 and day 10 after parturition, were used in the study. Glucose and BHB concentrations were determined immediately after sampling, while GPx activity was determined 24 hours after that.  Heifers with higher body condition had significantly higher average BHB concentration and GPx activity. Average glucose concentrations did not differ between groups.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze
T1  - The effect of body condition score of heifers on glucose and B-hydroxybutyrate concentrations and gluthatione peroxidase activity
VL  - 19
IS  - 2
SP  - 251
EP  - 264
DO  - 10.7251/VETJEN1902258N
ER  - 

@article{
author = "Nikšić, Aleksandar and Ječmenica, Jovana and Valčić, Olivera and Milanović, Svetlana",
year = "2019",
abstract = "Cilj ovog rada je bio da se utvrdi da li postoje promene u koncentraciji glukoze, beta-hidroksibutirata (BHB) i aktivnosti glutation-peroksidaze (GPx) u zavisnosti od telesne kondicije kod junica. Junice su na osnovu telesne kondicije (TK) svrstane u dve grupe: 1. TK=3,75 i 2. TK ≥4,0. U ogledu je korišćena puna krv 23 junice uzeta 1-10 dan nakon partusa. Koncentracije glukoze i BHB određivane su odmah po uzorkovanju krvi, a aktivnost GPX određivana je nakon 24 sata. Junice sa većom telesnom kondicijom imale su statistički značajno veću prosečnu koncentraciju BHB i aktivnost GPX. Prosečna koncentracija glukoze u obe grupe se nije razlikovala., The aim of this study was to establish if there are differences in glucose and ß-hydroxybutyrate (BHB) concentrations as well as glutathione peroxidase (GPx) activity related to the body condition in heifers. Based on the body condition (BC), heifers were divided into two groups: 1. BC=3.75 and 2. BC≥4.0. Whole blood samples from 23 heifers, taken between day 1 and day 10 after parturition, were used in the study. Glucose and BHB concentrations were determined immediately after sampling, while GPx activity was determined 24 hours after that.  Heifers with higher body condition had significantly higher average BHB concentration and GPx activity. Average glucose concentrations did not differ between groups.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze, The effect of body condition score of heifers on glucose and B-hydroxybutyrate concentrations and gluthatione peroxidase activity",
volume = "19",
number = "2",
pages = "251-264",
doi = "10.7251/VETJEN1902258N"
}

Nikšić, A., Ječmenica, J., Valčić, O.,& Milanović, S.. (2019). Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 19(2), 251-264.
https://doi.org/10.7251/VETJEN1902258N

Nikšić A, Ječmenica J, Valčić O, Milanović S. Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze. in Veterinarski žurnal Republike Srpske. 2019;19(2):251-264.
doi:10.7251/VETJEN1902258N .

Nikšić, Aleksandar, Ječmenica, Jovana, Valčić, Olivera, Milanović, Svetlana, "Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze" in Veterinarski žurnal Republike Srpske, 19, no. 2 (2019):251-264,
https://doi.org/10.7251/VETJEN1902258N . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Fratrić, Natalija
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3412
AB  - U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Biohemijske karakteristike kolostruma
SP  - 112
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3412
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana and Fratrić, Natalija",
year = "2019",
abstract = "U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Biohemijske karakteristike kolostruma",
pages = "112-121",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3412"
}

Valčić, O., Milanović, S.,& Fratrić, N.. (2019). Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412

Valčić O, Milanović S, Fratrić N. Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

Valčić, Olivera, Milanović, Svetlana, Fratrić, Natalija, "Biohemijske karakteristike kolostruma" in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):112-121,
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

TY  - JOUR
AU  - Marković, Radmila
AU  - Ćirić, Jelena
AU  - Drljačić, Aleksandar
AU  - Šefer, Dragan
AU  - Jovanović, Ivan B.
AU  - Jovanović, Dragoljub
AU  - Milanović, Svetlana
AU  - Trbović, Dejana
AU  - Radulović, Stamen
AU  - Baltić, Milan Ž.
AU  - Starcević, Marija
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1595
AB  - The present study was conducted to assess effects of selenium (Se)-yeast supplementation on glutathione peroxidase activity, Se levels in tissues, growth performance, carcass, and meat composition in broilers. A total of 275 one-d-old Cobb 500 broilers of both sexes were randomly allotted to 1 of 5 treatments during a 42-d period. The 5 treatments differed only in Se content: group 1 had no additional Se (background only); groups 2, 3, and 4 received 0.3 mg/kg of added Se from the beginning of the trial until d 21, whereas in the second half of the study (from d 22 to 42), these groups received 0.3, 0.6, and 0.9 mg/kg of added Se, respectively; and group 5 received 0.9 mg/kg of Se for the entire experimental period. At the end of the study, the control group showed significantly lower (P < 0.01) glutathione peroxidase activity in blood plasma compared to Se-supplemented groups. Regarding Se concentration in various tissues, the groups receiving Se yeast showed higher plasma, feces, and meat Se contents than the control group (P < 0.01). Supplementation of Se improved broilers body weight, weight gain and feed conversion ratio (P < 0.01). Dressing percentage was lower in the control group and the group with 0.3 mg/kg of added Se compared to other experimental groups (0.6 and 0.9 mg/kg of dietary Se). The proportion of less valuable carcass parts (wings and legs) was higher (P < 0.01) in the group fed the basal diet compared to groups supplemented with 0.9 mg/kg of Se. Initial and ultimate pH values differed among experimental groups (P < 0.05). Supplementation of Se improved the broilers antioxidative resistance, growth performance, carcass quality, and chemical composition of meat.
PB  - Oxford Univ Press, Oxford
T2  - Poultry Science
T1  - The effects of dietary Selenium-yeast level on glutathione peroxidase activity, tissue Selenium content, growth performance, and carcass and meat quality of broilers
VL  - 97
IS  - 8
SP  - 2861
EP  - 2870
DO  - 10.3382/ps/pey117
ER  - 

@article{
author = "Marković, Radmila and Ćirić, Jelena and Drljačić, Aleksandar and Šefer, Dragan and Jovanović, Ivan B. and Jovanović, Dragoljub and Milanović, Svetlana and Trbović, Dejana and Radulović, Stamen and Baltić, Milan Ž. and Starcević, Marija",
year = "2018",
abstract = "The present study was conducted to assess effects of selenium (Se)-yeast supplementation on glutathione peroxidase activity, Se levels in tissues, growth performance, carcass, and meat composition in broilers. A total of 275 one-d-old Cobb 500 broilers of both sexes were randomly allotted to 1 of 5 treatments during a 42-d period. The 5 treatments differed only in Se content: group 1 had no additional Se (background only); groups 2, 3, and 4 received 0.3 mg/kg of added Se from the beginning of the trial until d 21, whereas in the second half of the study (from d 22 to 42), these groups received 0.3, 0.6, and 0.9 mg/kg of added Se, respectively; and group 5 received 0.9 mg/kg of Se for the entire experimental period. At the end of the study, the control group showed significantly lower (P < 0.01) glutathione peroxidase activity in blood plasma compared to Se-supplemented groups. Regarding Se concentration in various tissues, the groups receiving Se yeast showed higher plasma, feces, and meat Se contents than the control group (P < 0.01). Supplementation of Se improved broilers body weight, weight gain and feed conversion ratio (P < 0.01). Dressing percentage was lower in the control group and the group with 0.3 mg/kg of added Se compared to other experimental groups (0.6 and 0.9 mg/kg of dietary Se). The proportion of less valuable carcass parts (wings and legs) was higher (P < 0.01) in the group fed the basal diet compared to groups supplemented with 0.9 mg/kg of Se. Initial and ultimate pH values differed among experimental groups (P < 0.05). Supplementation of Se improved the broilers antioxidative resistance, growth performance, carcass quality, and chemical composition of meat.",
publisher = "Oxford Univ Press, Oxford",
journal = "Poultry Science",
title = "The effects of dietary Selenium-yeast level on glutathione peroxidase activity, tissue Selenium content, growth performance, and carcass and meat quality of broilers",
volume = "97",
number = "8",
pages = "2861-2870",
doi = "10.3382/ps/pey117"
}

Marković, R., Ćirić, J., Drljačić, A., Šefer, D., Jovanović, I. B., Jovanović, D., Milanović, S., Trbović, D., Radulović, S., Baltić, M. Ž.,& Starcević, M.. (2018). The effects of dietary Selenium-yeast level on glutathione peroxidase activity, tissue Selenium content, growth performance, and carcass and meat quality of broilers. in Poultry Science
Oxford Univ Press, Oxford., 97(8), 2861-2870.
https://doi.org/10.3382/ps/pey117

Marković R, Ćirić J, Drljačić A, Šefer D, Jovanović IB, Jovanović D, Milanović S, Trbović D, Radulović S, Baltić MŽ, Starcević M. The effects of dietary Selenium-yeast level on glutathione peroxidase activity, tissue Selenium content, growth performance, and carcass and meat quality of broilers. in Poultry Science. 2018;97(8):2861-2870.
doi:10.3382/ps/pey117 .

Marković, Radmila, Ćirić, Jelena, Drljačić, Aleksandar, Šefer, Dragan, Jovanović, Ivan B., Jovanović, Dragoljub, Milanović, Svetlana, Trbović, Dejana, Radulović, Stamen, Baltić, Milan Ž., Starcević, Marija, "The effects of dietary Selenium-yeast level on glutathione peroxidase activity, tissue Selenium content, growth performance, and carcass and meat quality of broilers" in Poultry Science, 97, no. 8 (2018):2861-2870,
https://doi.org/10.3382/ps/pey117 . .

TY  - JOUR
AU  - Trifković, Julijana
AU  - Jovanović, Ljubomir
AU  - Đurić, Miloje
AU  - Stevanovic-Đorđević, Snežana
AU  - Milanović, Svetlana
AU  - Lazarević, Miodrag
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1669
AB  - Season may affect calves thermal comfort and behavior, but the data related to the overall influence of seasonal variations on dams colostrum and postnatal adaptive capability of calves are limited. The aim of this study was to measure the effects of a 49-day-long low air temperature (LAT) season (5.20 +/- 0.46 A degrees C mean air temperature) and a 53-day-long high air temperature (HAT) season (27.40 +/- 0.39 A degrees C mean air temperature) on dams colostrum quality and physiological, biochemical, hormonal, and oxidative stress parameters of their calves during the first 7 days of life. The dams colostrum was sampled at 2, 14, and 26 h after calving, before feeding of their calves. Calves blood samples were taken before the first colostrum intake and on days 1, 2, 3, and 7 of life. Calves physiological parameters were measured on days 0 and 7. HAT season significantly reduced the quality of dams colostrum. The ingestion of the low-quality colostrum, combined with the thermal discomfort during HAT season, probably provoked impaired physiological, biochemical, hormonal, and oxidative stress parameters in samples taken from the post-colostral calves. Additionally, intravenous glucose tolerance test was performed on day 7, which suggested an enhanced insulin response in HAT season calves. This study highlights the importance of adequate supporting strategies for the care of the late gestation cows and postnatal calves during the HAT season.
PB  - Springer, New York
T2  - International Journal of Biometeorology
T1  - Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves
VL  - 62
IS  - 6
SP  - 1097
EP  - 1108
DO  - 10.1007/s00484-018-1514-6
ER  - 

@article{
author = "Trifković, Julijana and Jovanović, Ljubomir and Đurić, Miloje and Stevanovic-Đorđević, Snežana and Milanović, Svetlana and Lazarević, Miodrag and Sladojević, Željko and Kirovski, Danijela",
year = "2018",
abstract = "Season may affect calves thermal comfort and behavior, but the data related to the overall influence of seasonal variations on dams colostrum and postnatal adaptive capability of calves are limited. The aim of this study was to measure the effects of a 49-day-long low air temperature (LAT) season (5.20 +/- 0.46 A degrees C mean air temperature) and a 53-day-long high air temperature (HAT) season (27.40 +/- 0.39 A degrees C mean air temperature) on dams colostrum quality and physiological, biochemical, hormonal, and oxidative stress parameters of their calves during the first 7 days of life. The dams colostrum was sampled at 2, 14, and 26 h after calving, before feeding of their calves. Calves blood samples were taken before the first colostrum intake and on days 1, 2, 3, and 7 of life. Calves physiological parameters were measured on days 0 and 7. HAT season significantly reduced the quality of dams colostrum. The ingestion of the low-quality colostrum, combined with the thermal discomfort during HAT season, probably provoked impaired physiological, biochemical, hormonal, and oxidative stress parameters in samples taken from the post-colostral calves. Additionally, intravenous glucose tolerance test was performed on day 7, which suggested an enhanced insulin response in HAT season calves. This study highlights the importance of adequate supporting strategies for the care of the late gestation cows and postnatal calves during the HAT season.",
publisher = "Springer, New York",
journal = "International Journal of Biometeorology",
title = "Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves",
volume = "62",
number = "6",
pages = "1097-1108",
doi = "10.1007/s00484-018-1514-6"
}

Trifković, J., Jovanović, L., Đurić, M., Stevanovic-Đorđević, S., Milanović, S., Lazarević, M., Sladojević, Ž.,& Kirovski, D.. (2018). Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves. in International Journal of Biometeorology
Springer, New York., 62(6), 1097-1108.
https://doi.org/10.1007/s00484-018-1514-6

Trifković J, Jovanović L, Đurić M, Stevanovic-Đorđević S, Milanović S, Lazarević M, Sladojević Ž, Kirovski D. Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves. in International Journal of Biometeorology. 2018;62(6):1097-1108.
doi:10.1007/s00484-018-1514-6 .

Trifković, Julijana, Jovanović, Ljubomir, Đurić, Miloje, Stevanovic-Đorđević, Snežana, Milanović, Svetlana, Lazarević, Miodrag, Sladojević, Željko, Kirovski, Danijela, "Influence of different seasons during late gestation on Holstein cows  colostrum and postnatal adaptive capability of their calves" in International Journal of Biometeorology, 62, no. 6 (2018):1097-1108,
https://doi.org/10.1007/s00484-018-1514-6 . .

TY  - JOUR
AU  - Bošnjaković, Dušan
AU  - Petrović, Aleksandra
AU  - Valčić, Olivera
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2164
AB  - Cilj rada je bio da se utvrdi stabilnost aktivnosti glutation peroksidaza 1 i 3 tokom skladištenja na temperaturi +4°C i -18°C. Krv je uzeta od osam ovaca, a aktivnost enzima je određivana u plazmi (GPx3) i eritrocitima (GPx1) prvog, trećeg, petog i sedmog dana u uzorcima čuvanim na +4°C i nakon jednog i tri meseca u uzorcima čuvanim na -18°C. Aktivnost GPx3 je tokom skladištenja na ovim temperaturama statistički značajno opala dok je aktivnost GPx1 ostala ista nakon tri meseca čuvanja na temperaturi -18°C. Rezultati ovog rada ukazuju na to da se GPx3 mora određivati u svežim uzorcima plazme ovaca, dok se aktivnost GPx1 eritrocita ovaca može određivati i nakon 3 meseca čuvanja na temperaturi -18°C.
AB  - The aim of the study was to determine the stability of the activity of glutathione peroxidases 1 and 3 during storage at +4°C and -18°C. Blood samples were taken from eight sheep and the activity of the enzyme was determined in the plasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day in samples stored at +4°C and after one and three months in samples stored at -18°C. GPx3 activity decreased significantly during storage at both temperatures, while GPx1 remained steady even after three months of storage at -18°C. Obtained results indicate that GPx3 activity has to be determined in fresh sheep plasma samples, while the activity of sheep erythrocyte GPx1 can be determined even after 3 months of storage at -18°C.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca
T1  - The effects of different storing temperatures on the activity of sheep blood and plasma glutathione peroxidase
VL  - 18
IS  - 1
SP  - 38
EP  - 53
DO  - 10.7251/VETJ1801038B
ER  - 

@article{
author = "Bošnjaković, Dušan and Petrović, Aleksandra and Valčić, Olivera and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2018",
abstract = "Cilj rada je bio da se utvrdi stabilnost aktivnosti glutation peroksidaza 1 i 3 tokom skladištenja na temperaturi +4°C i -18°C. Krv je uzeta od osam ovaca, a aktivnost enzima je određivana u plazmi (GPx3) i eritrocitima (GPx1) prvog, trećeg, petog i sedmog dana u uzorcima čuvanim na +4°C i nakon jednog i tri meseca u uzorcima čuvanim na -18°C. Aktivnost GPx3 je tokom skladištenja na ovim temperaturama statistički značajno opala dok je aktivnost GPx1 ostala ista nakon tri meseca čuvanja na temperaturi -18°C. Rezultati ovog rada ukazuju na to da se GPx3 mora određivati u svežim uzorcima plazme ovaca, dok se aktivnost GPx1 eritrocita ovaca može određivati i nakon 3 meseca čuvanja na temperaturi -18°C., The aim of the study was to determine the stability of the activity of glutathione peroxidases 1 and 3 during storage at +4°C and -18°C. Blood samples were taken from eight sheep and the activity of the enzyme was determined in the plasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day in samples stored at +4°C and after one and three months in samples stored at -18°C. GPx3 activity decreased significantly during storage at both temperatures, while GPx1 remained steady even after three months of storage at -18°C. Obtained results indicate that GPx3 activity has to be determined in fresh sheep plasma samples, while the activity of sheep erythrocyte GPx1 can be determined even after 3 months of storage at -18°C.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca, The effects of different storing temperatures on the activity of sheep blood and plasma glutathione peroxidase",
volume = "18",
number = "1",
pages = "38-53",
doi = "10.7251/VETJ1801038B"
}

Bošnjaković, D., Petrović, A., Valčić, O., Jovanović, I. B.,& Milanović, S.. (2018). Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 18(1), 38-53.
https://doi.org/10.7251/VETJ1801038B

Bošnjaković D, Petrović A, Valčić O, Jovanović IB, Milanović S. Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca. in Veterinarski žurnal Republike Srpske. 2018;18(1):38-53.
doi:10.7251/VETJ1801038B .

Bošnjaković, Dušan, Petrović, Aleksandra, Valčić, Olivera, Jovanović, Ivan B., Milanović, Svetlana, "Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca" in Veterinarski žurnal Republike Srpske, 18, no. 1 (2018):38-53,
https://doi.org/10.7251/VETJ1801038B . .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Radovanović, Anita
AU  - Milošević, Ivan
AU  - Lužajić Božinovski, Tijana
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
AU  - Korać, Aleksandra
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2597
AB  - The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.
PB  - Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology
PB  - Belgrade : Ministry of Education, Science and Technological Development
PB  - Belgrade : University of Belgrade
PB  - Belgrade : Faculty of Biology of University of Belgrade
C3  - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
T1  - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
SP  - 103
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2597
ER  - 

@conference{
author = "Danilović Luković, Jelena and Radovanović, Anita and Milošević, Ivan and Lužajić Božinovski, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra",
year = "2018",
abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.",
publisher = "Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology, Belgrade : Ministry of Education, Science and Technological Development, Belgrade : University of Belgrade, Belgrade : Faculty of Biology of University of Belgrade",
journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade",
title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection",
pages = "103-103",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2597"
}

Danilović Luković, J., Radovanović, A., Milošević, I., Lužajić Božinovski, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology., 103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597

Danilović Luković J, Radovanović A, Milošević I, Lužajić Božinovski T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

Danilović Luković, Jelena, Radovanović, Anita, Milošević, Ivan, Lužajić Božinovski, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):103-103,
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3441
AB  - Komunikacija među živim svetom je vrlo kompleksan proces i može se odvijati na nekoliko načina. Najzastupljeniji oblici komunikacije među životinjama su vizuelna, auditivna i hemijska komunikacija. Kod ptica se smatra da
su najzastupljeniji vizuelni vidovi komunikacije, pa zatim auditivni. Do skoro
se vrlo malo pridavalo značaju proučavanja komunikacije kod ptica pomoću
hemijskih materija (mirisa i feromona) zbog slabo razvijenog olfaktornog bulbusa kod ovih kičmenjaka, kao i odsustva vomeronazalnog organa. Međutim,
novija istraživanja ukazuju na postojanje specifičnih receptora za određene hemijske materije i kod ptica. Kod nekih vrsta ptica, produkt uropigealne žlezde
sadrži materije koje mogu imati odlike feromona i koji nose informaciju o vrsti,
polu, identitetu, populaciji i poreklu. Kod sisara je ovaj vid komunikacije veoma
zastupljen, a kako su neki feromoni već izolovani i sintetski se proizvode, uveliko se koriste i u farmskoj proizvodnji. Zbog toga smo smatrali da je korisno
razmotriti razne mogućnosti primene feromona i u živinarskoj proizvodnji.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXVII Savetovanje živinara, Tara, 2018
T1  - Moguća primena feromona u živinarskoj proizvodnji
SP  - 37
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3441
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2018",
abstract = "Komunikacija među živim svetom je vrlo kompleksan proces i može se odvijati na nekoliko načina. Najzastupljeniji oblici komunikacije među životinjama su vizuelna, auditivna i hemijska komunikacija. Kod ptica se smatra da
su najzastupljeniji vizuelni vidovi komunikacije, pa zatim auditivni. Do skoro
se vrlo malo pridavalo značaju proučavanja komunikacije kod ptica pomoću
hemijskih materija (mirisa i feromona) zbog slabo razvijenog olfaktornog bulbusa kod ovih kičmenjaka, kao i odsustva vomeronazalnog organa. Međutim,
novija istraživanja ukazuju na postojanje specifičnih receptora za određene hemijske materije i kod ptica. Kod nekih vrsta ptica, produkt uropigealne žlezde
sadrži materije koje mogu imati odlike feromona i koji nose informaciju o vrsti,
polu, identitetu, populaciji i poreklu. Kod sisara je ovaj vid komunikacije veoma
zastupljen, a kako su neki feromoni već izolovani i sintetski se proizvode, uveliko se koriste i u farmskoj proizvodnji. Zbog toga smo smatrali da je korisno
razmotriti razne mogućnosti primene feromona i u živinarskoj proizvodnji.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXVII Savetovanje živinara, Tara, 2018",
title = "Moguća primena feromona u živinarskoj proizvodnji",
pages = "37-45",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3441"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2018). Moguća primena feromona u živinarskoj proizvodnji. in XXVII Savetovanje živinara, Tara, 2018
Beograd : Ciiip Živinarstvo., 37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3441

Milanović S, Valčić O, Jovanović I. Moguća primena feromona u živinarskoj proizvodnji. in XXVII Savetovanje živinara, Tara, 2018. 2018;:37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3441 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Moguća primena feromona u živinarskoj proizvodnji" in XXVII Savetovanje živinara, Tara, 2018 (2018):37-45,
https://hdl.handle.net/21.15107/rcub_veterinar_3441 .

TY  - JOUR
AU  - Suluburić, Adam
AU  - Milanović, Svetlana
AU  - Vranješ-Đurić, Sanja
AU  - Jovanović, Ivan B.
AU  - Barna, Tomislav
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Szenci, Otto
AU  - Gvozdić, Dragan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1486
AB  - Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase
VL  - 65
IS  - 3
SP  - 446
EP  - 458
DO  - 10.1556/004.2017.042
ER  - 

@article{
author = "Suluburić, Adam and Milanović, Svetlana and Vranješ-Đurić, Sanja and Jovanović, Ivan B. and Barna, Tomislav and Stojić, Milica and Fratrić, Natalija and Szenci, Otto and Gvozdić, Dragan",
year = "2017",
abstract = "Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase",
volume = "65",
number = "3",
pages = "446-458",
doi = "10.1556/004.2017.042"
}

Suluburić, A., Milanović, S., Vranješ-Đurić, S., Jovanović, I. B., Barna, T., Stojić, M., Fratrić, N., Szenci, O.,& Gvozdić, D.. (2017). Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica
Akademiai Kiado Rt, Budapest., 65(3), 446-458.
https://doi.org/10.1556/004.2017.042

Suluburić A, Milanović S, Vranješ-Đurić S, Jovanović IB, Barna T, Stojić M, Fratrić N, Szenci O, Gvozdić D. Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica. 2017;65(3):446-458.
doi:10.1556/004.2017.042 .

Suluburić, Adam, Milanović, Svetlana, Vranješ-Đurić, Sanja, Jovanović, Ivan B., Barna, Tomislav, Stojić, Milica, Fratrić, Natalija, Szenci, Otto, Gvozdić, Dragan, "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase" in Acta Veterinaria Hungarica, 65, no. 3 (2017):446-458,
https://doi.org/10.1556/004.2017.042 . .

TY  - JOUR
AU  - Bjelica, Jelena
AU  - Jovanović, Ivan B.
AU  - Ilić, Vesna
AU  - Valčić, Olivera
AU  - Kirovski, Danijela
AU  - Gvozdić, Dragan
AU  - Milanović, Svetlana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1535
AB  - Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats
VL  - 67
IS  - 3
SP  - 340
EP  - 355
DO  - 10.1515/acve-2017-0028
ER  - 

@article{
author = "Bjelica, Jelena and Jovanović, Ivan B. and Ilić, Vesna and Valčić, Olivera and Kirovski, Danijela and Gvozdić, Dragan and Milanović, Svetlana",
year = "2017",
abstract = "Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats",
volume = "67",
number = "3",
pages = "340-355",
doi = "10.1515/acve-2017-0028"
}

Bjelica, J., Jovanović, I. B., Ilić, V., Valčić, O., Kirovski, D., Gvozdić, D.,& Milanović, S.. (2017). Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 340-355.
https://doi.org/10.1515/acve-2017-0028

Bjelica J, Jovanović IB, Ilić V, Valčić O, Kirovski D, Gvozdić D, Milanović S. Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd. 2017;67(3):340-355.
doi:10.1515/acve-2017-0028 .

Bjelica, Jelena, Jovanović, Ivan B., Ilić, Vesna, Valčić, Olivera, Kirovski, Danijela, Gvozdić, Dragan, Milanović, Svetlana, "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats" in Acta Veterinaria-Beograd, 67, no. 3 (2017):340-355,
https://doi.org/10.1515/acve-2017-0028 . .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2159
AB  - Актуелни трендови употребе биљних производа у заштити здравља људи и
животиња довели су до широке примене природних полифенола присутних у
биљкама, као што су зелени чај, боровница, аронија, итд. На основу досадашњих
испитивања настанка оксидативног стреса као и начина превенције, можемо
закључити да су грожђе, семенке грожђа, као и комина показали одређене протективне ефекте. In vivo студије ефикасности полифенола су изузетно комплексне и до сада су дале контроверзне резултате. У овде представљеном раду дат је
преглед до сада постигнутих сазнања ефеката употребе полифенола грожђа у
заштити од оксидативног стреса домаћих животиња.
AB  - Current trends on the use of plant products in the protection of human and animal
health have resulted in the widespread use of natural polyphenols present in plants
such as green tea, chokeberries, blackberries, etc. According to a wide array of trials
on oxidative stress and its prevention it can be concluded that grapes, grapeseeds and pomace have some protective effects. In vivo polyphenol efficacy studies are extremely
complex and up to date have raised a number of controversial results. In the here
presented paper we have offered a review on the knowledge on grape polyphenols in
oxidative stress protection of domestic animals.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa
T1  - Grape polyphenols in the protection of domestic animals from oxidative stress
VL  - 17
IS  - 2
SP  - 182
EP  - 191
DO  - 10.7251/VETJ1702182V
ER  - 

@article{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2017",
abstract = "Актуелни трендови употребе биљних производа у заштити здравља људи и
животиња довели су до широке примене природних полифенола присутних у
биљкама, као што су зелени чај, боровница, аронија, итд. На основу досадашњих
испитивања настанка оксидативног стреса као и начина превенције, можемо
закључити да су грожђе, семенке грожђа, као и комина показали одређене протективне ефекте. In vivo студије ефикасности полифенола су изузетно комплексне и до сада су дале контроверзне резултате. У овде представљеном раду дат је
преглед до сада постигнутих сазнања ефеката употребе полифенола грожђа у
заштити од оксидативног стреса домаћих животиња., Current trends on the use of plant products in the protection of human and animal
health have resulted in the widespread use of natural polyphenols present in plants
such as green tea, chokeberries, blackberries, etc. According to a wide array of trials
on oxidative stress and its prevention it can be concluded that grapes, grapeseeds and pomace have some protective effects. In vivo polyphenol efficacy studies are extremely
complex and up to date have raised a number of controversial results. In the here
presented paper we have offered a review on the knowledge on grape polyphenols in
oxidative stress protection of domestic animals.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa, Grape polyphenols in the protection of domestic animals from oxidative stress",
volume = "17",
number = "2",
pages = "182-191",
doi = "10.7251/VETJ1702182V"
}

Valčić, O.,& Milanović, S.. (2017). Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(2), 182-191.
https://doi.org/10.7251/VETJ1702182V

Valčić O, Milanović S. Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa. in Veterinarski žurnal Republike Srpske. 2017;17(2):182-191.
doi:10.7251/VETJ1702182V .

Valčić, Olivera, Milanović, Svetlana, "Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa" in Veterinarski žurnal Republike Srpske, 17, no. 2 (2017):182-191,
https://doi.org/10.7251/VETJ1702182V . .