Petrović, Tamaš

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Authority KeyName Variants
orcid::0000-0002-9164-3356
  • Petrović, Tamaš (46)
Projects
Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination Application of the EIIP/ISM bioinformatics platform in discovery of novel therapeutic targets and potential therapeutic molecules
Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production Research on pharmacological characteristics of antimicrobial agents, introduction of new technological solutions and alternative prophylactic methods with the purpose to improve control of infectious animal disease
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200031 (Scientific Institute of Veterinary Medicine 'Novi Sad', Novi Sad) Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens
The influence of the quality of the components of food for cyprinid fish species on the quality of meat, losses and the profitability of production Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200015 (University of Belgrade, Institute for Medical Research) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200143 (University of Belgrade, Faculty of Veterinary Medicine)
Studying climate change and its influence on environment: impacts, adaptation and mitigation Difuzni sistem zvezdastih (stellata) ćelija i njihova uloga u oštećenju tkiva, reparaciji i neoplastičnim procesima kod sisara
Biotehnološki postupci u etiološkoj dijagnostici virusnih i bakterijskih infekcija, kontaminanata hrane i bioloških proizvoda u veterinarskoj medicini Bacterial zoonoses-Development of molecular and immunological diagnostic methods and their standardization
Ecological and viral investigation on the presents of emerging zoonoses in national parks in republic of Serbia Provincial Secretariat for Higher Education and Scientific Research, Autonomous Province of Vojvodina, project number 142-451-3170/2022-01/2
Serbian Ministry of Education, Science and Technological Development Slovenian Ministry for Higher Education, Science and Technology [P4 0092]
Slovenian-Serbian bilateral project Veterinary Directorate, Ministry of Agriculture and Environment Protection, Republic of Serbia

Author's Bibliography

Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja

Despotović, Darko; Ilić, Tamara; Marić, Jelena; Subić, Ivona; Nikolić, Sonja; Gligorić, Srđan; Kasagić, Dragan; Stevanović, Oliver; Knežević, Dragan; Petrović, Tamaš

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2024)

TY  - CONF
AU  - Despotović, Darko
AU  - Ilić, Tamara
AU  - Marić, Jelena
AU  - Subić, Ivona
AU  - Nikolić, Sonja
AU  - Gligorić, Srđan
AU  - Kasagić, Dragan
AU  - Stevanović, Oliver
AU  - Knežević, Dragan
AU  - Petrović, Tamaš
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3893
AB  - Кардиопулмонална дирофилариоза је векторски преносиво обољење канида, фелида и других врста сисара, које проузрокује Dirofilaria immitis. У ендемским подручјима са високим преваленцијама ове нематоде, региструју се бројне инфекције људи праћене озбиљним оштећењима плућа. Пошто су дивљи месоједи резервоари, транспортери и потенцијални извор инфекције за домаће псе, циљ истраживања је да се на подручју Републике Српске утврди преваленција и дистрибуција инфекције D. immitis код дивљих карнивора. Током 2022., 2023. и почетком 2024. године вршена је редовна пасивна контрола дивљих карнивора на бјеснило и активна контрола успјешности вакцинације лисица против бјеснила. Тада су у Ветеринарски институт у Бања Луци достављани трупови легално одстрељених карнивора из цијеле Републике Српске, на којима је урађена циљана паразитолошка секција. Десно срце и плућне артерије су прегледане на присуство адулта D. immitis, а из срца и великих крвних судова узоркована је крв за серолошку и молекуларну дијагностику. Током 2022. код 148 обдукованих лисица и двије куне није дијагностикована D. immitis. Током 2023. испитане су 202 лисице, 22 шакала и седам куна, а адулти D. immitis су утврђени у 1,73% случајева (четири шакала из општине Брод). Током јануара и фебруара 2024. испитано је 117 лисица, 32 шакала и двије куне, а позитиван налаз је утврђен у 6,62% случајева (три шакала из општине Брод, пет шакала и двије лисице из општине Дервента). Добијени резултати пружају прелиминарни увид у географску дистрибуцију и преваленцију D. immitis код дивљих месоједа у Републици Српској. Познавање епидемиологије D. immitis у популацијама дивљих карнивора може помоћи да се разјасне ризици од могућности инфекције домаћих паса и људи овом нематодом. Током обдукције крв за даље претраге је узоркована селективно, тако да се постигне равномјерна заступљеност дивљих месоједа са свих географских подручја Републике Српске. Резултати серолошких и молекуларних анализа ће бити накнадно саопштени и употпуниће епизоотиолошку слику распрострањености D. immitis код дивљих карнивора у Републици Српској.
AB  - Cardiopulmonary dirofilariasis is a vector-borne disease of canids, felids and other mammals, caused by Dirofilaria immitis. In endemic areas with high prevalence of this nematode, numerous human infections accompanied by serious lung damage are registered. Since wild carnivores are reservoirs, transporters and potential sources of infection for domestic dogs, the aim of the research is to determine the prevalence and distribution of D. immitis infection in wild carnivores in the territory of the Republic of Srpska. During 2022, 2023 and the beginning of 2024, regular passive control of wild carnivores for rabies and active control of the success of vaccination of foxes against rabies were carried out. At that time, the carcasses of legally shot carnivores from all over the Republic of Srpska were delivered to the Veterinary Institute in Banja Luka, on which a targeted parasitological section was performed. The right heart and pulmonary arteries were examined for the presence of adult D. immitis, and blood was sampled from the heart and large blood vessels for serological and molecular diagnostics. During 2022, D. immitis was not diagnosed in 148 autopsied foxes and two martens. During 2023, 202 foxes, 22 jackals and seven martens were examined, and D. immitis adults were found in 1.73% of cases (four jackals from the municipality of Brod). During January and February 2024, 117 foxes, 32 jackals and two martens were examined, and positive findings were found in 6.62% of cases (three jackals from the municipality of Brod, five jackals and two foxes from the municipality of Derventa). The obtained results provide a preliminary insight into the geographical distribution and prevalence of D. immitis in wild carnivores in the Republic of Srpska. Knowledge of the epidemiology of D. immitis in wild carnivore populations may help clarify the risks of potential infection of domestic dogs and humans with this nematode. During the autopsy, blood for further tests was sampled selectively, so that an even representation of wild carnivores from all geographical areas of the Republic of Srpska was achieved. The results of serological and molecular analyzes will be announced later and will complete the epizootiological picture of the prevalence of D. immitis in wild carnivores in the Republic of Srpska.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja
T1  - Dirofilaria immitis in wild carnivores in the Republic of Srpska – preliminary results of the episootiological research
SP  - 38
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3893
ER  - 
@conference{
author = "Despotović, Darko and Ilić, Tamara and Marić, Jelena and Subić, Ivona and Nikolić, Sonja and Gligorić, Srđan and Kasagić, Dragan and Stevanović, Oliver and Knežević, Dragan and Petrović, Tamaš",
year = "2024",
abstract = "Кардиопулмонална дирофилариоза је векторски преносиво обољење канида, фелида и других врста сисара, које проузрокује Dirofilaria immitis. У ендемским подручјима са високим преваленцијама ове нематоде, региструју се бројне инфекције људи праћене озбиљним оштећењима плућа. Пошто су дивљи месоједи резервоари, транспортери и потенцијални извор инфекције за домаће псе, циљ истраживања је да се на подручју Републике Српске утврди преваленција и дистрибуција инфекције D. immitis код дивљих карнивора. Током 2022., 2023. и почетком 2024. године вршена је редовна пасивна контрола дивљих карнивора на бјеснило и активна контрола успјешности вакцинације лисица против бјеснила. Тада су у Ветеринарски институт у Бања Луци достављани трупови легално одстрељених карнивора из цијеле Републике Српске, на којима је урађена циљана паразитолошка секција. Десно срце и плућне артерије су прегледане на присуство адулта D. immitis, а из срца и великих крвних судова узоркована је крв за серолошку и молекуларну дијагностику. Током 2022. код 148 обдукованих лисица и двије куне није дијагностикована D. immitis. Током 2023. испитане су 202 лисице, 22 шакала и седам куна, а адулти D. immitis су утврђени у 1,73% случајева (четири шакала из општине Брод). Током јануара и фебруара 2024. испитано је 117 лисица, 32 шакала и двије куне, а позитиван налаз је утврђен у 6,62% случајева (три шакала из општине Брод, пет шакала и двије лисице из општине Дервента). Добијени резултати пружају прелиминарни увид у географску дистрибуцију и преваленцију D. immitis код дивљих месоједа у Републици Српској. Познавање епидемиологије D. immitis у популацијама дивљих карнивора може помоћи да се разјасне ризици од могућности инфекције домаћих паса и људи овом нематодом. Током обдукције крв за даље претраге је узоркована селективно, тако да се постигне равномјерна заступљеност дивљих месоједа са свих географских подручја Републике Српске. Резултати серолошких и молекуларних анализа ће бити накнадно саопштени и употпуниће епизоотиолошку слику распрострањености D. immitis код дивљих карнивора у Републици Српској., Cardiopulmonary dirofilariasis is a vector-borne disease of canids, felids and other mammals, caused by Dirofilaria immitis. In endemic areas with high prevalence of this nematode, numerous human infections accompanied by serious lung damage are registered. Since wild carnivores are reservoirs, transporters and potential sources of infection for domestic dogs, the aim of the research is to determine the prevalence and distribution of D. immitis infection in wild carnivores in the territory of the Republic of Srpska. During 2022, 2023 and the beginning of 2024, regular passive control of wild carnivores for rabies and active control of the success of vaccination of foxes against rabies were carried out. At that time, the carcasses of legally shot carnivores from all over the Republic of Srpska were delivered to the Veterinary Institute in Banja Luka, on which a targeted parasitological section was performed. The right heart and pulmonary arteries were examined for the presence of adult D. immitis, and blood was sampled from the heart and large blood vessels for serological and molecular diagnostics. During 2022, D. immitis was not diagnosed in 148 autopsied foxes and two martens. During 2023, 202 foxes, 22 jackals and seven martens were examined, and D. immitis adults were found in 1.73% of cases (four jackals from the municipality of Brod). During January and February 2024, 117 foxes, 32 jackals and two martens were examined, and positive findings were found in 6.62% of cases (three jackals from the municipality of Brod, five jackals and two foxes from the municipality of Derventa). The obtained results provide a preliminary insight into the geographical distribution and prevalence of D. immitis in wild carnivores in the Republic of Srpska. Knowledge of the epidemiology of D. immitis in wild carnivore populations may help clarify the risks of potential infection of domestic dogs and humans with this nematode. During the autopsy, blood for further tests was sampled selectively, so that an even representation of wild carnivores from all geographical areas of the Republic of Srpska was achieved. The results of serological and molecular analyzes will be announced later and will complete the epizootiological picture of the prevalence of D. immitis in wild carnivores in the Republic of Srpska.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja, Dirofilaria immitis in wild carnivores in the Republic of Srpska – preliminary results of the episootiological research",
pages = "38-39",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3893"
}
Despotović, D., Ilić, T., Marić, J., Subić, I., Nikolić, S., Gligorić, S., Kasagić, D., Stevanović, O., Knežević, D.,& Petrović, T.. (2024). Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 38-39.
https://hdl.handle.net/21.15107/rcub_veterinar_3893
Despotović D, Ilić T, Marić J, Subić I, Nikolić S, Gligorić S, Kasagić D, Stevanović O, Knežević D, Petrović T. Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:38-39.
https://hdl.handle.net/21.15107/rcub_veterinar_3893 .
Despotović, Darko, Ilić, Tamara, Marić, Jelena, Subić, Ivona, Nikolić, Sonja, Gligorić, Srđan, Kasagić, Dragan, Stevanović, Oliver, Knežević, Dragan, Petrović, Tamaš, "Dirofilaria immitis kod divljih karnivora u Republici Srpskoj – preliminarni rezultati epizootiološkog istraživanja" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):38-39,
https://hdl.handle.net/21.15107/rcub_veterinar_3893 .

Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“

Nedić, Drago; Petrović, Tamaš; Petrović, Miloš; Rodić Vukmir, Nina; Dimitrijević, Dragana; Laušević, Dejan; Toplak, Ivan; Fejzić, Nihad; Pendovski, Lazo; Cvetnić, Željko

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2023)

TY  - CONF
AU  - Nedić, Drago
AU  - Petrović, Tamaš
AU  - Petrović, Miloš
AU  - Rodić Vukmir, Nina
AU  - Dimitrijević, Dragana
AU  - Laušević, Dejan
AU  - Toplak, Ivan
AU  - Fejzić, Nihad
AU  - Pendovski, Lazo
AU  - Cvetnić, Željko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3062
AB  - Концепт „Једно здравље“ препознаје међусобну повезаност здравља људи,
животиња и животне средине. Наглашава важност сарадње између више
сектора, укључујући медицину, ветеринарску медицину и науку о животној
средини, како би се промовисало здравље и добробит свих. Једно здравље
настоји да спријечи и контролише ширење болести које се могу пренијети
између животиња и људи, као што је зооноза COVID-19, праћењем и
истраживањем епидемија у обе популације. Здравље животне средине може
утицати на здравље људи и животиња, а Једно здравље наглашава важност
заштите животне средине како би се спречило ширење болести и унапредило
опште здравље. Концепт „Једно здравље“ представља холистички приступ
унапређењу здравља и благостања, признајући да су здравље људи, животиња и
животне средине међусобно повезани и међузависни. Неке од најопаснијих
зооноза на свијету су ебола, вирус Марбург, грозница Ласа, вирус Нипах,
грозница долине Рифт и Крим-Конго хеморагична грозница. Ове болести имају
значајан утицај на јавно здравље широм свијета и изазвале су више избијања и
пандемија у прошлости. Антимикробна резистенција је критичан здравствени
изазов који представља пријетњу по јавно здравље, а повезана је са употребом
антибиотика у здрављу људи и животиња. То може довести до развоја бактерија
отпорних на антибиотике које је тешко лијечити, чинећи инфекције тежим, па
чак и опасним по живот. Безбедност хране је још један суштински аспект Једног
здравља, јер контаминирана храна може довести до ширења болести које се
преносе храном, што представља значајну пријетњу по јавно здравље. Надлежне
институције имају кључну улогу у обезбеђивању да храна буде безбедна за
конзумацију примјеном прописа, спречавањем контаминације и инспекцијом. Мјере заштите животне средине су такође суштински аспект Једног здравља,
пошто фактори животне средине могу утицати на здравље људи, животиња и
екосистема. Надлежне институције треба да раде заједно на развоју и примјени
мјера за заштиту животне средине, смањење загађења и ублажавање утицаја
климатских промјена.
Надлежне институције, укључујући владине агенције, јавне здравствене
организације и академске институције, играју кључну улогу у имплементацији
концепта једног здравља у циљу рјешавања глобалних здравствених изазова као
што су зоонозе, отпорност на антимикробне лијекове, безбедност хране и
заштита животне средине. Њихове улоге и задаци укључују:
Сарадња и координација: Надлежне институције треба да раде заједно на
подстицању међусекторске сарадње и координације како би се интегрисали
подаци о здрављу људи, животиња и животне средине ради откривања,
превенције и одговора на нове здравствене пријетње.
Надзор и рано откривање: Надлежне институције треба да успоставе и
одржавају системе надзора за откривање и праћење ширења зоонотских
болести, отпорности на антимикробне лијекове и болести које се преносе
храном, као и новонасталих пријетњи по здравље људи, животиња и животне
средине.
Истраживање: Надлежне институције треба да подрже и спроводе истраживања
како би боље разумјели основне узроке зоонотских болести, отпорности на
антимикробне лијекове и болести које се преносе храном, и да развију ефикасне
интервенције за ублажавање њиховог утицаја.
Образовање и свијест: Надлежне институције треба да промовишу јавно
образовање и свијест о принципима Једног здравља и њиховом значају у
решавању здравствених изазова, укључујући зоонозе, отпорност на
антимикробне лијекове, безбедност хране и заштиту животне средине.
Развој политике и прописивање: Надлежне институције треба да развију
политике и прописе који подржавају приступ Једног здравља и промовишу
интегрисане приступе за решавање здравствених изазова и заштите животне
средине.
Концепт Jедно здравље jе подржан на нивоу међународних организациjа кроз
трипартитни меморандум (WOAH, WHO и FAO). Од 2022. године
меморандумом о сарадњи се придружила агенциjа Уједињених нација за
животну средину (UNEP). Међутим, институционална сарадња на нивоу
чланица и држава је jош далеко од очекиване или jе нема никако. Та сарадња
ниjе препозната ни у легислативи коjа регулише дјелатност ветеринарске и
хумане медицине и здравља околине.
Укратко, надлежне институције играју кључну улогу у имплементацији
концепта Једно здравље за решавање глобалних здравствених изазова,
укључујући зоонозе, отпорност на антимикробне лијекове, безбедност хране и
заштиту животне средине. Радећи заједно и испуњавајући своје улоге и задатке,
они могу помоћи у промовисању здравља и добробити људи, животиња и
животне средине.
AB  - The concept of One Health recognizes that human, animal, and environmental health
are interconnected. It emphasizes the importance of collaboration among different
sectors, including medicine, veterinary medicine, and environmental science, to
promote the health and well-being of all. One Health seeks to prevent and control the
spread of diseases that can be transmitted between animals and humans, such as
zoonotic diseases like COVID -19, by monitoring and studying outbreaks in both
populations. Environmental health can impact human and animal health, and One
Health emphasizes the importance of protecting the environment to prevent the spread
of disease and promote overall health. The One Health concept represents a holistic
approach to promoting health and well-being and recognizes that human, animal, and
environmental health are interconnected and interdependent. Among the world's most
dangerous zoonotic diseases is Ebola, Marburg virus, Lassa fever, Nipah virus, Rift
Valley fever, and Crimean-Congo hemorrhagic fever. These diseases have a
significant public health impact worldwide and have caused several outbreaks and
pandemics in the past. Antimicrobial resistance is a critical problem that threatens
public health and is related to the use of antibiotics in human and veterinary medicine.
This can lead to the development of antibiotic-resistant bacteria that are difficult to
treat and make infections worse and even life-threatening. Food safety is another
essential aspect of One Health, as contaminated food can lead to the spread of
foodborne illness, posing a significant public health threat. Competent institutions
play a critical role in ensuring food is safe by enforcing regulations, conducting
inspections, and taking steps to prevent contamination. Environmental protection
measures are also an essential aspect of One Health, as environmental factors can
affect the health of humans, animals and ecosystems. Relevant institutions should work together to develop and implement measures to protect the environment, reduce
pollution, and mitigate the effects of climate change.
Relevant institutions, including government agencies, public health organizations,
and academic institutions, play a critical role in implementing the One Health concept
to address global health challenges such as zoonotic diseases, antimicrobial resistance,
food safety, and environmental protection. Their roles and responsibilities include:
Collaboration and coordination: relevant agencies should work together to promote
intersectoral collaboration and coordination to integrate human, animal, and
environmental health data to identify, prevent, and respond to emerging health threats.
Surveillance and Early Detection: Competent institutions should establish and
maintain surveillance systems to detect and monitor the spread of zoonoses,
antimicrobial resistance, and foodborne diseases, as well as emerging threats to
human, animal, and environmental health.
Research: relevant institutions should support and conduct research to better
understand the causes of zoonoses, antimicrobial resistance, and foodborne diseases
and develop effective measures to mitigate their impact.
Education and awareness: relevant agencies should promote public education and
awareness of One Health principles and their importance in addressing health
challenges, including zoonotic diseases, antimicrobial resistance, food safety, and
environmental protection.
Policy development and regulation: relevant institutions should develop policies and
regulations that support the One Health approach and promote integrated approaches
to addressing health challenges and environmental protection.
The One Health concept is supported at the level of international organizations by a
tripartite memorandum (WOAH, WHO, and FAO). As of 2022, the United Nations
Environment Program (UNEP) has joined with a memorandum of cooperation.
However, institutional cooperation at the member state and country level is still far
from what is expected or even non-existent. This cooperation is not even recognized
in the legislation that regulates the activities of veterinary medicine, human medicine
and environmental health.
In summary, relevant institutions play a critical role in implementing the One Health
approach to address global health challenges, including zoonotic diseases,
antimicrobial resistance, food safety, and environmental protection. By working
together and fulfilling their roles and responsibilities, they can help promote the health
and well-being of people, animals, and the environment.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“
T1  - The role and tasks of competent institutions in implementing the concept of „One health“
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3062
ER  - 
@conference{
author = "Nedić, Drago and Petrović, Tamaš and Petrović, Miloš and Rodić Vukmir, Nina and Dimitrijević, Dragana and Laušević, Dejan and Toplak, Ivan and Fejzić, Nihad and Pendovski, Lazo and Cvetnić, Željko",
year = "2023",
abstract = "Концепт „Једно здравље“ препознаје међусобну повезаност здравља људи,
животиња и животне средине. Наглашава важност сарадње између више
сектора, укључујући медицину, ветеринарску медицину и науку о животној
средини, како би се промовисало здравље и добробит свих. Једно здравље
настоји да спријечи и контролише ширење болести које се могу пренијети
између животиња и људи, као што је зооноза COVID-19, праћењем и
истраживањем епидемија у обе популације. Здравље животне средине може
утицати на здравље људи и животиња, а Једно здравље наглашава важност
заштите животне средине како би се спречило ширење болести и унапредило
опште здравље. Концепт „Једно здравље“ представља холистички приступ
унапређењу здравља и благостања, признајући да су здравље људи, животиња и
животне средине међусобно повезани и међузависни. Неке од најопаснијих
зооноза на свијету су ебола, вирус Марбург, грозница Ласа, вирус Нипах,
грозница долине Рифт и Крим-Конго хеморагична грозница. Ове болести имају
значајан утицај на јавно здравље широм свијета и изазвале су више избијања и
пандемија у прошлости. Антимикробна резистенција је критичан здравствени
изазов који представља пријетњу по јавно здравље, а повезана је са употребом
антибиотика у здрављу људи и животиња. То може довести до развоја бактерија
отпорних на антибиотике које је тешко лијечити, чинећи инфекције тежим, па
чак и опасним по живот. Безбедност хране је још један суштински аспект Једног
здравља, јер контаминирана храна може довести до ширења болести које се
преносе храном, што представља значајну пријетњу по јавно здравље. Надлежне
институције имају кључну улогу у обезбеђивању да храна буде безбедна за
конзумацију примјеном прописа, спречавањем контаминације и инспекцијом. Мјере заштите животне средине су такође суштински аспект Једног здравља,
пошто фактори животне средине могу утицати на здравље људи, животиња и
екосистема. Надлежне институције треба да раде заједно на развоју и примјени
мјера за заштиту животне средине, смањење загађења и ублажавање утицаја
климатских промјена.
Надлежне институције, укључујући владине агенције, јавне здравствене
организације и академске институције, играју кључну улогу у имплементацији
концепта једног здравља у циљу рјешавања глобалних здравствених изазова као
што су зоонозе, отпорност на антимикробне лијекове, безбедност хране и
заштита животне средине. Њихове улоге и задаци укључују:
Сарадња и координација: Надлежне институције треба да раде заједно на
подстицању међусекторске сарадње и координације како би се интегрисали
подаци о здрављу људи, животиња и животне средине ради откривања,
превенције и одговора на нове здравствене пријетње.
Надзор и рано откривање: Надлежне институције треба да успоставе и
одржавају системе надзора за откривање и праћење ширења зоонотских
болести, отпорности на антимикробне лијекове и болести које се преносе
храном, као и новонасталих пријетњи по здравље људи, животиња и животне
средине.
Истраживање: Надлежне институције треба да подрже и спроводе истраживања
како би боље разумјели основне узроке зоонотских болести, отпорности на
антимикробне лијекове и болести које се преносе храном, и да развију ефикасне
интервенције за ублажавање њиховог утицаја.
Образовање и свијест: Надлежне институције треба да промовишу јавно
образовање и свијест о принципима Једног здравља и њиховом значају у
решавању здравствених изазова, укључујући зоонозе, отпорност на
антимикробне лијекове, безбедност хране и заштиту животне средине.
Развој политике и прописивање: Надлежне институције треба да развију
политике и прописе који подржавају приступ Једног здравља и промовишу
интегрисане приступе за решавање здравствених изазова и заштите животне
средине.
Концепт Jедно здравље jе подржан на нивоу међународних организациjа кроз
трипартитни меморандум (WOAH, WHO и FAO). Од 2022. године
меморандумом о сарадњи се придружила агенциjа Уједињених нација за
животну средину (UNEP). Међутим, институционална сарадња на нивоу
чланица и држава је jош далеко од очекиване или jе нема никако. Та сарадња
ниjе препозната ни у легислативи коjа регулише дјелатност ветеринарске и
хумане медицине и здравља околине.
Укратко, надлежне институције играју кључну улогу у имплементацији
концепта Једно здравље за решавање глобалних здравствених изазова,
укључујући зоонозе, отпорност на антимикробне лијекове, безбедност хране и
заштиту животне средине. Радећи заједно и испуњавајући своје улоге и задатке,
они могу помоћи у промовисању здравља и добробити људи, животиња и
животне средине., The concept of One Health recognizes that human, animal, and environmental health
are interconnected. It emphasizes the importance of collaboration among different
sectors, including medicine, veterinary medicine, and environmental science, to
promote the health and well-being of all. One Health seeks to prevent and control the
spread of diseases that can be transmitted between animals and humans, such as
zoonotic diseases like COVID -19, by monitoring and studying outbreaks in both
populations. Environmental health can impact human and animal health, and One
Health emphasizes the importance of protecting the environment to prevent the spread
of disease and promote overall health. The One Health concept represents a holistic
approach to promoting health and well-being and recognizes that human, animal, and
environmental health are interconnected and interdependent. Among the world's most
dangerous zoonotic diseases is Ebola, Marburg virus, Lassa fever, Nipah virus, Rift
Valley fever, and Crimean-Congo hemorrhagic fever. These diseases have a
significant public health impact worldwide and have caused several outbreaks and
pandemics in the past. Antimicrobial resistance is a critical problem that threatens
public health and is related to the use of antibiotics in human and veterinary medicine.
This can lead to the development of antibiotic-resistant bacteria that are difficult to
treat and make infections worse and even life-threatening. Food safety is another
essential aspect of One Health, as contaminated food can lead to the spread of
foodborne illness, posing a significant public health threat. Competent institutions
play a critical role in ensuring food is safe by enforcing regulations, conducting
inspections, and taking steps to prevent contamination. Environmental protection
measures are also an essential aspect of One Health, as environmental factors can
affect the health of humans, animals and ecosystems. Relevant institutions should work together to develop and implement measures to protect the environment, reduce
pollution, and mitigate the effects of climate change.
Relevant institutions, including government agencies, public health organizations,
and academic institutions, play a critical role in implementing the One Health concept
to address global health challenges such as zoonotic diseases, antimicrobial resistance,
food safety, and environmental protection. Their roles and responsibilities include:
Collaboration and coordination: relevant agencies should work together to promote
intersectoral collaboration and coordination to integrate human, animal, and
environmental health data to identify, prevent, and respond to emerging health threats.
Surveillance and Early Detection: Competent institutions should establish and
maintain surveillance systems to detect and monitor the spread of zoonoses,
antimicrobial resistance, and foodborne diseases, as well as emerging threats to
human, animal, and environmental health.
Research: relevant institutions should support and conduct research to better
understand the causes of zoonoses, antimicrobial resistance, and foodborne diseases
and develop effective measures to mitigate their impact.
Education and awareness: relevant agencies should promote public education and
awareness of One Health principles and their importance in addressing health
challenges, including zoonotic diseases, antimicrobial resistance, food safety, and
environmental protection.
Policy development and regulation: relevant institutions should develop policies and
regulations that support the One Health approach and promote integrated approaches
to addressing health challenges and environmental protection.
The One Health concept is supported at the level of international organizations by a
tripartite memorandum (WOAH, WHO, and FAO). As of 2022, the United Nations
Environment Program (UNEP) has joined with a memorandum of cooperation.
However, institutional cooperation at the member state and country level is still far
from what is expected or even non-existent. This cooperation is not even recognized
in the legislation that regulates the activities of veterinary medicine, human medicine
and environmental health.
In summary, relevant institutions play a critical role in implementing the One Health
approach to address global health challenges, including zoonotic diseases,
antimicrobial resistance, food safety, and environmental protection. By working
together and fulfilling their roles and responsibilities, they can help promote the health
and well-being of people, animals, and the environment.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“, The role and tasks of competent institutions in implementing the concept of „One health“",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3062"
}
Nedić, D., Petrović, T., Petrović, M., Rodić Vukmir, N., Dimitrijević, D., Laušević, D., Toplak, I., Fejzić, N., Pendovski, L.,& Cvetnić, Ž.. (2023). Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 24-27.
https://hdl.handle.net/21.15107/rcub_veterinar_3062
Nedić D, Petrović T, Petrović M, Rodić Vukmir N, Dimitrijević D, Laušević D, Toplak I, Fejzić N, Pendovski L, Cvetnić Ž. Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:24-27.
https://hdl.handle.net/21.15107/rcub_veterinar_3062 .
Nedić, Drago, Petrović, Tamaš, Petrović, Miloš, Rodić Vukmir, Nina, Dimitrijević, Dragana, Laušević, Dejan, Toplak, Ivan, Fejzić, Nihad, Pendovski, Lazo, Cvetnić, Željko, "Uloga i zadaci nadležnih institucija u primeni koncepta „Jedno zdravlje“" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):24-27,
https://hdl.handle.net/21.15107/rcub_veterinar_3062 .

Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform

Tešović, Bojana; Nišavić, Jakov; Banović-Đeri, Bojana; Petrović, Tamaš; Radalj, Andrea; Šekler, Milanko; Matović, Kazimir; Debeljak, Zoran; Vasković, Nikola; Dmitrić, Marko; Vidanović, Dejan

(Elsevier, 2023)

TY  - JOUR
AU  - Tešović, Bojana
AU  - Nišavić, Jakov
AU  - Banović-Đeri, Bojana
AU  - Petrović, Tamaš
AU  - Radalj, Andrea
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Debeljak, Zoran
AU  - Vasković, Nikola
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2520
AB  - West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.
PB  - Elsevier
T2  - Diagnostic Microbiology and Infectious Disease
T1  - Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform
VL  - 105
IS  - 2
SP  - 115852
DO  - 10.1016/j.diagmicrobio.2022.115852
ER  - 
@article{
author = "Tešović, Bojana and Nišavić, Jakov and Banović-Đeri, Bojana and Petrović, Tamaš and Radalj, Andrea and Šekler, Milanko and Matović, Kazimir and Debeljak, Zoran and Vasković, Nikola and Dmitrić, Marko and Vidanović, Dejan",
year = "2023",
abstract = "West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.",
publisher = "Elsevier",
journal = "Diagnostic Microbiology and Infectious Disease",
title = "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform",
volume = "105",
number = "2",
pages = "115852",
doi = "10.1016/j.diagmicrobio.2022.115852"
}
Tešović, B., Nišavić, J., Banović-Đeri, B., Petrović, T., Radalj, A., Šekler, M., Matović, K., Debeljak, Z., Vasković, N., Dmitrić, M.,& Vidanović, D.. (2023). Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease
Elsevier., 105(2), 115852.
https://doi.org/10.1016/j.diagmicrobio.2022.115852
Tešović B, Nišavić J, Banović-Đeri B, Petrović T, Radalj A, Šekler M, Matović K, Debeljak Z, Vasković N, Dmitrić M, Vidanović D. Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease. 2023;105(2):115852.
doi:10.1016/j.diagmicrobio.2022.115852 .
Tešović, Bojana, Nišavić, Jakov, Banović-Đeri, Bojana, Petrović, Tamaš, Radalj, Andrea, Šekler, Milanko, Matović, Kazimir, Debeljak, Zoran, Vasković, Nikola, Dmitrić, Marko, Vidanović, Dejan, "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform" in Diagnostic Microbiology and Infectious Disease, 105, no. 2 (2023):115852,
https://doi.org/10.1016/j.diagmicrobio.2022.115852 . .
4

Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year

Đurđević, Biljana; Vučićević, Ivana; Polaček, Vladimir; Pajić, Marko; Knežević, Slobodan; Petrović, Tamaš; Grubač, Siniša; Gajdov, Vladimir; Aleksić-Kovačević, Sanja

(Scientific Veterinary Institute Novi Sad, 2023)

TY  - CONF
AU  - Đurđević, Biljana
AU  - Vučićević, Ivana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Knežević, Slobodan
AU  - Petrović, Tamaš
AU  - Grubač, Siniša
AU  - Gajdov, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3365
AB  - The highly pathogenic avian influenza (HPAI) epidemic in the 2021/2022 epidemiological year was the most
widespread in Europe, and HPAI H5N1 was by far the predominant virus type reported. In Serbia, since early
2021 and 2022, multiple outbreaks of HPAI have occurred. The H5N1 subtype was also dominant during this
epizootic, although a few cases of H5N8 subtype infection were reported in mute swans. This epizootic
affected backyard chickens (3 outbreaks), and the virus was also detected in wild birds, mostly in mute swans
(4 outbreaks). All cases were reported in the north of Serbia. Here we describe the pathological findings of
natural HPAI H5N1 infection in poultry species (chickens and turkeys) and wild birds (mute swans) that died
during this epizootic. Routine necropsies were carried out on the carcasses of 15 mute swans, 15 chickens,
and four turkeys. The external body and internal organs were examined grossly, and the gross pathology was
recorded and photographed. Body condition was estimated based on the amount of body fat and
musculature. Tissue samples of the brain, pancreas, spleen, and lungs were collected, and the supernatants
of the tissue homogenates were used for molecular diagnosis by RT-qPCR method. The affected birds showed
nervous manifestations (abnormal head position, tremors, leg paralysis) and all birds were in good condition.
The presence of influenza virus was detected in tissue samples of all tested animals. The external macroscopic
changes included cyanosis and necrosis in the crest and wattle, and these lesions were more pronounced in
chickens. In mute swans, there were no external lesions. The H5N1 HPAI virus produced several consistent
gross lesions among the species investigated. Foremost among these lesions was: multifocal pancreatic
necrosis and hemorrhages, petechial hemorrhages in coelomic and epicardial fat and epicardial petechiae.
The lungs showed moderate (turkeys) to severe (chickens and mute swans) diffuse congestion and oedema.
In most cases, mild splenomegaly and spleen necrosis were noted. Additionally, gizzard or proventricular
lesions were not observed in any bird. The natural HPAI H5N1 infection in poultry and mute swans showed
similar clinical disease, including neurological disorders, as well as similar pathologic presentation involving
necrotic lesions and vascular damage, primarily affecting the pancreas and myocardium. Evaluating the
pathological presentation of natural disease is particularly important in emerging infectious diseases such as
influenza A virus, in which different strains can have different pathogenicity and clinical presentations.
PB  - Scientific Veterinary Institute Novi Sad
C3  - 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023
T1  - Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3365
ER  - 
@conference{
author = "Đurđević, Biljana and Vučićević, Ivana and Polaček, Vladimir and Pajić, Marko and Knežević, Slobodan and Petrović, Tamaš and Grubač, Siniša and Gajdov, Vladimir and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "The highly pathogenic avian influenza (HPAI) epidemic in the 2021/2022 epidemiological year was the most
widespread in Europe, and HPAI H5N1 was by far the predominant virus type reported. In Serbia, since early
2021 and 2022, multiple outbreaks of HPAI have occurred. The H5N1 subtype was also dominant during this
epizootic, although a few cases of H5N8 subtype infection were reported in mute swans. This epizootic
affected backyard chickens (3 outbreaks), and the virus was also detected in wild birds, mostly in mute swans
(4 outbreaks). All cases were reported in the north of Serbia. Here we describe the pathological findings of
natural HPAI H5N1 infection in poultry species (chickens and turkeys) and wild birds (mute swans) that died
during this epizootic. Routine necropsies were carried out on the carcasses of 15 mute swans, 15 chickens,
and four turkeys. The external body and internal organs were examined grossly, and the gross pathology was
recorded and photographed. Body condition was estimated based on the amount of body fat and
musculature. Tissue samples of the brain, pancreas, spleen, and lungs were collected, and the supernatants
of the tissue homogenates were used for molecular diagnosis by RT-qPCR method. The affected birds showed
nervous manifestations (abnormal head position, tremors, leg paralysis) and all birds were in good condition.
The presence of influenza virus was detected in tissue samples of all tested animals. The external macroscopic
changes included cyanosis and necrosis in the crest and wattle, and these lesions were more pronounced in
chickens. In mute swans, there were no external lesions. The H5N1 HPAI virus produced several consistent
gross lesions among the species investigated. Foremost among these lesions was: multifocal pancreatic
necrosis and hemorrhages, petechial hemorrhages in coelomic and epicardial fat and epicardial petechiae.
The lungs showed moderate (turkeys) to severe (chickens and mute swans) diffuse congestion and oedema.
In most cases, mild splenomegaly and spleen necrosis were noted. Additionally, gizzard or proventricular
lesions were not observed in any bird. The natural HPAI H5N1 infection in poultry and mute swans showed
similar clinical disease, including neurological disorders, as well as similar pathologic presentation involving
necrotic lesions and vascular damage, primarily affecting the pancreas and myocardium. Evaluating the
pathological presentation of natural disease is particularly important in emerging infectious diseases such as
influenza A virus, in which different strains can have different pathogenicity and clinical presentations.",
publisher = "Scientific Veterinary Institute Novi Sad",
journal = "15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023",
title = "Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3365"
}
Đurđević, B., Vučićević, I., Polaček, V., Pajić, M., Knežević, S., Petrović, T., Grubač, S., Gajdov, V.,& Aleksić-Kovačević, S.. (2023). Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year. in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023
Scientific Veterinary Institute Novi Sad..
https://hdl.handle.net/21.15107/rcub_veterinar_3365
Đurđević B, Vučićević I, Polaček V, Pajić M, Knežević S, Petrović T, Grubač S, Gajdov V, Aleksić-Kovačević S. Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year. in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023. 2023;.
https://hdl.handle.net/21.15107/rcub_veterinar_3365 .
Đurđević, Biljana, Vučićević, Ivana, Polaček, Vladimir, Pajić, Marko, Knežević, Slobodan, Petrović, Tamaš, Grubač, Siniša, Gajdov, Vladimir, Aleksić-Kovačević, Sanja, "Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year" in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023 (2023),
https://hdl.handle.net/21.15107/rcub_veterinar_3365 .

Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia

Đurđević, Biljana; Polaček, Vladimir; Pajić, Marko; Petrović, Tamaš; Vučićević, Ivana; Vidanović, Dejan; Aleksić-Kovačević, Sanja

(MDPI, 2023)

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Petrović, Tamaš
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2706
AB  - In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.
PB  - MDPI
T2  - Animals
T1  - Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
VL  - 13
IS  - 4
SP  - 700
DO  - 10.3390/ani13040700
ER  - 
@article{
author = "Đurđević, Biljana and Polaček, Vladimir and Pajić, Marko and Petrović, Tamaš and Vučićević, Ivana and Vidanović, Dejan and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.",
publisher = "MDPI",
journal = "Animals",
title = "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia",
volume = "13",
number = "4",
pages = "700",
doi = "10.3390/ani13040700"
}
Đurđević, B., Polaček, V., Pajić, M., Petrović, T., Vučićević, I., Vidanović, D.,& Aleksić-Kovačević, S.. (2023). Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals
MDPI., 13(4), 700.
https://doi.org/10.3390/ani13040700
Đurđević B, Polaček V, Pajić M, Petrović T, Vučićević I, Vidanović D, Aleksić-Kovačević S. Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals. 2023;13(4):700.
doi:10.3390/ani13040700 .
Đurđević, Biljana, Polaček, Vladimir, Pajić, Marko, Petrović, Tamaš, Vučićević, Ivana, Vidanović, Dejan, Aleksić-Kovačević, Sanja, "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia" in Animals, 13, no. 4 (2023):700,
https://doi.org/10.3390/ani13040700 . .
6

Primjena koncepta „Jedno zdravlje“ - šanse i prednosti

Nedić, Drago; Petrović, Tamaš; Rodić Vukmir, Nina; Dimitrijević, Dragana; Đurić, Boban; Toplak, Ivan; Cvetnić, Željko

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2022)

TY  - CONF
AU  - Nedić, Drago
AU  - Petrović, Tamaš
AU  - Rodić Vukmir, Nina
AU  - Dimitrijević, Dragana
AU  - Đurić, Boban
AU  - Toplak, Ivan
AU  - Cvetnić, Željko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3063
AB  - Скоро двије трећине узрочника болести код људи је поријеклом од домаћих и
дивљих животиња, познате као зоонозе. Многе болести представљају ризик за
јавно здравље широм свијета и због тога је императив да се ове болести
сузбијају на локалном па и глобалном нивоу. Концепт „једно здравље“ заснован
је на свијести о потреби за заштиту јавног здравља дјелујући превасходно на
превенцији и контроли патогена унутар хумане и животињске популација у
животној средини у којој живе.
Концепт ”једно здравље” се почео примјењивати након потписивања
заједничког документа између Свјетске здравствене организације (СЗО),
Светске организације за здравље животиња (ОИЕ) и Организације за храну и
пољопривреду Уједињених нација (ФАО). Тим документом су наведене
узајамне одговорности и циљеви у овој области. Од самог почетка, за
приоритетне теме заједничког дјеловања постављени су: вирус бјеснила, од
којег годишње умире преко 59.000 људи, затим друге вирусе зоонотског
карактера као што је птичји грип. Међу прве приоритете уврштена је и
антимикробна резистенција. Све неопходне активности стручњака из области
здравља животиња, јавног здравља и животне средине треба да се примјењују
на локалном, националном и глобалном нивоу. То би требало да допринесе
сталном и истовременом унапређењу јавног здравља, здравља животиња и
заштите животне средине широм свијета.
Најефикасније и најекономичније решење за заштиту људи је борба против свих
зоонотских патогена у животињској популацији. То захтијева стратешки
приступ, организовање, управљање и контролу уз адекватна улагање
финансијских средстава и ангажовање расположивих људских и материјалних
ресурса и из јавног и приватног сектора. То подразумијева да се успоставе нови
механизми међусобног информисања и синхронизованог заједничког
дјеловања. Свакако треба водити рачуна и о патогенима који нису зоонозе, али
имају индиректни негативан утицај на здравље, производњу хране и животну
средину. Све три међународне организације објављују стандарде и препоруке о поступању у одређеним околностима које су од помоћи надлежним
институцијама за доношење одлука.
И поред тога што је концепт ”једно здравље” успостављен прије пар деценија
ипак се на терену не примјећује промјена политике у овом погледу. Веома мали
број земаља је донио стратегију по концепту ”једно здравље”, односно неке
земље су урадиле веома мало док је огроман број земаља које нису учиниле
ништа. Све ово се може објаснити на појави пандемије COVID 19 која траје
више од двије године са веома много нејасних ситуација као што је поријекло
вируса, мутације, дијагностика и вакцинација. Поражавајућа је чињеница да је
учешће ветеринарских институција у периоду пандемије било веома
незапажено. У неким земљама су формиране комисије или ад-хок радне групе
за зонозе, али није било системског приступа по концепту ”једно здравље”.
У овом раду се наводе различита искуства појединих земаља код појава
одређених болести као што су COVID 19, бјеснило и птичји грип у региону,
затим искуства у контроли антимикробне резистенције. Циљ рада је да се укаже
на предности као и шансе код примјене концепта ”једно здравље”, на добрим и
лошим искуствима на локалном, регионалном и глобалном нивоу.
AB  - Nearly two-thirds of human pathogens come from domestic and wild animals, known
as zoonoses. Many diseases pose a risk to public health around the world and it is
therefore imperative that these diseases be eradicated locally and globally. The
concept of "One Health" is based on awareness of the need to protect public health,
acting primarily on the prevention and control of pathogens within human and animal
populations in the environment in which they live.
The concept of "One Health" began to be applied after the signing of a joint document
between the World Health Organization (WHO), the World Organization for Animal
Health (RES) and the Food and Agriculture Organization of the United Nations
(FAO). This document sets out mutual responsibilities and goals in this area. From
the very beginning, the priority topics for joint action were: the rabies virus, which
kills over 59,000 people a year, and other zoonotic viruses such as bird flu.
Antimicrobial resistance is one of the first priorities. All necessary activities of experts
in the field of animal health, public health and the environment should be implemented
at the local, national and global levels. This should contribute to the continuous and
simultaneous improvement of public health, animal health and environmental
protection worldwide.
The most efficient and economical solution for human protection is the fight against
all zoonotic pathogens in the animal population. This requires a strategic approach,
organization, management and control with adequate investment of financial
resources and engagement of available human and material resources from both the
public and private sectors. This means establishing new mechanisms for mutual
information and synchronized joint action. Pathogens that are not zoonoses, but have
an indirect negative impact on health, food production and the environment, should
certainly be taken into account. All three international organizations publish standards
and recommendations on how to act in certain circumstances that are helpful to the
competent decision-making institutions . Despite the fact that the concept of "One Health" was established a few decades ago,
there is no change in policy in this regard. Very few countries have adopted a strategy
based on the concept of "One Health", i.e. some countries have done very little while
a huge number of countries have done nothing. All this can be explained by the
appearance of the COVID 19 pandemic, which has been going on for more than two
years, with many unclear situations such as the origin of the virus, mutations,
diagnostics and vaccinations. It is a devastating fact that the involvement of veterinary
institutions during the pandemic period was quite unnoticed. In some countries,
commissions or ad-hoc working groups on zonoses have been established, but there
has been no systematic approach to the concept of "One Health".
This paper presents different experiences of individual countries in the occurrence of
certain diseases such as COVID 19, rabies and bird flu in the region, as well as
experiences in the control of antimicrobial resistance. The aim of this paper is to point
out the advantages and opportunities in the application of the concept of "One Health",
the good and bad experiences at the local, regional and global levels.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
T1  - Primjena koncepta „Jedno zdravlje“ - šanse i prednosti
T1  - Application of the concept of "One Health" - opportunities and benefits
SP  - 28
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3063
ER  - 
@conference{
author = "Nedić, Drago and Petrović, Tamaš and Rodić Vukmir, Nina and Dimitrijević, Dragana and Đurić, Boban and Toplak, Ivan and Cvetnić, Željko",
year = "2022",
abstract = "Скоро двије трећине узрочника болести код људи је поријеклом од домаћих и
дивљих животиња, познате као зоонозе. Многе болести представљају ризик за
јавно здравље широм свијета и због тога је императив да се ове болести
сузбијају на локалном па и глобалном нивоу. Концепт „једно здравље“ заснован
је на свијести о потреби за заштиту јавног здравља дјелујући превасходно на
превенцији и контроли патогена унутар хумане и животињске популација у
животној средини у којој живе.
Концепт ”једно здравље” се почео примјењивати након потписивања
заједничког документа између Свјетске здравствене организације (СЗО),
Светске организације за здравље животиња (ОИЕ) и Организације за храну и
пољопривреду Уједињених нација (ФАО). Тим документом су наведене
узајамне одговорности и циљеви у овој области. Од самог почетка, за
приоритетне теме заједничког дјеловања постављени су: вирус бјеснила, од
којег годишње умире преко 59.000 људи, затим друге вирусе зоонотског
карактера као што је птичји грип. Међу прве приоритете уврштена је и
антимикробна резистенција. Све неопходне активности стручњака из области
здравља животиња, јавног здравља и животне средине треба да се примјењују
на локалном, националном и глобалном нивоу. То би требало да допринесе
сталном и истовременом унапређењу јавног здравља, здравља животиња и
заштите животне средине широм свијета.
Најефикасније и најекономичније решење за заштиту људи је борба против свих
зоонотских патогена у животињској популацији. То захтијева стратешки
приступ, организовање, управљање и контролу уз адекватна улагање
финансијских средстава и ангажовање расположивих људских и материјалних
ресурса и из јавног и приватног сектора. То подразумијева да се успоставе нови
механизми међусобног информисања и синхронизованог заједничког
дјеловања. Свакако треба водити рачуна и о патогенима који нису зоонозе, али
имају индиректни негативан утицај на здравље, производњу хране и животну
средину. Све три међународне организације објављују стандарде и препоруке о поступању у одређеним околностима које су од помоћи надлежним
институцијама за доношење одлука.
И поред тога што је концепт ”једно здравље” успостављен прије пар деценија
ипак се на терену не примјећује промјена политике у овом погледу. Веома мали
број земаља је донио стратегију по концепту ”једно здравље”, односно неке
земље су урадиле веома мало док је огроман број земаља које нису учиниле
ништа. Све ово се може објаснити на појави пандемије COVID 19 која траје
више од двије године са веома много нејасних ситуација као што је поријекло
вируса, мутације, дијагностика и вакцинација. Поражавајућа је чињеница да је
учешће ветеринарских институција у периоду пандемије било веома
незапажено. У неким земљама су формиране комисије или ад-хок радне групе
за зонозе, али није било системског приступа по концепту ”једно здравље”.
У овом раду се наводе различита искуства појединих земаља код појава
одређених болести као што су COVID 19, бјеснило и птичји грип у региону,
затим искуства у контроли антимикробне резистенције. Циљ рада је да се укаже
на предности као и шансе код примјене концепта ”једно здравље”, на добрим и
лошим искуствима на локалном, регионалном и глобалном нивоу., Nearly two-thirds of human pathogens come from domestic and wild animals, known
as zoonoses. Many diseases pose a risk to public health around the world and it is
therefore imperative that these diseases be eradicated locally and globally. The
concept of "One Health" is based on awareness of the need to protect public health,
acting primarily on the prevention and control of pathogens within human and animal
populations in the environment in which they live.
The concept of "One Health" began to be applied after the signing of a joint document
between the World Health Organization (WHO), the World Organization for Animal
Health (RES) and the Food and Agriculture Organization of the United Nations
(FAO). This document sets out mutual responsibilities and goals in this area. From
the very beginning, the priority topics for joint action were: the rabies virus, which
kills over 59,000 people a year, and other zoonotic viruses such as bird flu.
Antimicrobial resistance is one of the first priorities. All necessary activities of experts
in the field of animal health, public health and the environment should be implemented
at the local, national and global levels. This should contribute to the continuous and
simultaneous improvement of public health, animal health and environmental
protection worldwide.
The most efficient and economical solution for human protection is the fight against
all zoonotic pathogens in the animal population. This requires a strategic approach,
organization, management and control with adequate investment of financial
resources and engagement of available human and material resources from both the
public and private sectors. This means establishing new mechanisms for mutual
information and synchronized joint action. Pathogens that are not zoonoses, but have
an indirect negative impact on health, food production and the environment, should
certainly be taken into account. All three international organizations publish standards
and recommendations on how to act in certain circumstances that are helpful to the
competent decision-making institutions . Despite the fact that the concept of "One Health" was established a few decades ago,
there is no change in policy in this regard. Very few countries have adopted a strategy
based on the concept of "One Health", i.e. some countries have done very little while
a huge number of countries have done nothing. All this can be explained by the
appearance of the COVID 19 pandemic, which has been going on for more than two
years, with many unclear situations such as the origin of the virus, mutations,
diagnostics and vaccinations. It is a devastating fact that the involvement of veterinary
institutions during the pandemic period was quite unnoticed. In some countries,
commissions or ad-hoc working groups on zonoses have been established, but there
has been no systematic approach to the concept of "One Health".
This paper presents different experiences of individual countries in the occurrence of
certain diseases such as COVID 19, rabies and bird flu in the region, as well as
experiences in the control of antimicrobial resistance. The aim of this paper is to point
out the advantages and opportunities in the application of the concept of "One Health",
the good and bad experiences at the local, regional and global levels.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022",
title = "Primjena koncepta „Jedno zdravlje“ - šanse i prednosti, Application of the concept of "One Health" - opportunities and benefits",
pages = "28-31",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3063"
}
Nedić, D., Petrović, T., Rodić Vukmir, N., Dimitrijević, D., Đurić, B., Toplak, I.,& Cvetnić, Ž.. (2022). Primjena koncepta „Jedno zdravlje“ - šanse i prednosti. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 28-31.
https://hdl.handle.net/21.15107/rcub_veterinar_3063
Nedić D, Petrović T, Rodić Vukmir N, Dimitrijević D, Đurić B, Toplak I, Cvetnić Ž. Primjena koncepta „Jedno zdravlje“ - šanse i prednosti. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022. 2022;:28-31.
https://hdl.handle.net/21.15107/rcub_veterinar_3063 .
Nedić, Drago, Petrović, Tamaš, Rodić Vukmir, Nina, Dimitrijević, Dragana, Đurić, Boban, Toplak, Ivan, Cvetnić, Željko, "Primjena koncepta „Jedno zdravlje“ - šanse i prednosti" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022 (2022):28-31,
https://hdl.handle.net/21.15107/rcub_veterinar_3063 .

Critical analysis of the application of the concept „One health“

Nedić, Drago; Petrović, Tamaš; Rodić Vukmir, Nina; Dragana, Dimitrijević; Đurić, Boban; Toplak, Ivan; Laušević, Dejan; Cvetnić, Željko

(Veterinarska komora Republike Srpske, 2022)

TY  - CONF
AU  - Nedić, Drago
AU  - Petrović, Tamaš
AU  - Rodić Vukmir, Nina
AU  - Dragana, Dimitrijević
AU  - Đurić, Boban
AU  - Toplak, Ivan
AU  - Laušević, Dejan
AU  - Cvetnić, Željko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2484
AB  - Uzročnici infektivnih bolesti ljudi su preko 60% porijeklom od životinja što ove bolesti svrstava u zoonoze. Zbog toga je imperativ da se ove bolesti suzbijaju na lokalnom pa i globalnom nivou. Koncept „jedno zdravlje“ zasnovan je na svijesti o potrebi za zaštitu javnog zdravlja djelujući prevashodno na prevenciji i kontroli patogena unutar humane i životinjske populacija u životnoj sredini u kojoj žive. Koncept ”jedno zdravlje” se počeo primjenjivati nakon potpisivanja zajedničkog dokumenta između Svjetske zdravstvene organizacije (SZO), Svetske organizacije za zdravlje životinja (OIE) i Organizacije za hranu i poljoprivredu Ujedinjenih nacija (FAO). Tim dokumentom su navedene uzajamne odgovornosti i ciljevi u ovoj oblasti. Od samog početka, za prioritetne teme zajedničkog djelovanja postavljeni su: virus bjesnila, od kojeg godišnje umire preko 59.000 ljudi, zatim druge viruse zoonotskog karaktera kao što je ptičji grip. Među prve prioritete uvrštena je i antimikrobna rezistencija. Sve neophodne aktivnosti stručnjaka iz oblasti zdravlja životinja, javnog zdravlja i životne sredine treba da se primjenjuju na lokalnom, nacionalnom i globalnom nivou. To bi trebalo da doprinese stalnom i istovremenom unapređenju javnog zdravlja, zdravlja životinja i zaštite životne sredine širom svijeta. Najekonomičnije i najefikasnije rešenje za zaštitu ljudi je borba protiv svih zoonotskih patogena u životinjskoj populaciji. To zahtijeva strateški pristup, organizovanje, upravljanje i kontrolu uz adekvatna ulaganje finansijskih sredstava i angažovanje raspoloživih ljudskih i materijalnih resursa i iz javnog i privatnog sektora. To podrazimijeva da se uspostave novi mehanizmi međusobnog informisanja i sinhronizovanog zajedničkog djelovanja. Svakako treba voditi računa i o patogenima koji nisu zoonoze, ali imaju indirektni negativan uticaj na zdravlje, proizvodnju hrane i životnu sredinu. Sve tri međunarodne organizacije objavljuju standarde i preporuke o postupanju u određenim okolnostima koje su od pomoći nadležnim institucijama za donošenje odluka. I pored toga što je koncept ”jedno zdravlje” uspostavljen prije par decenija ipak se na terenu ne primjećuje promjena politike u ovom pogledu. Veoma mali broj zemalja je donio strategiju po konceptu ”jedno zdravlje”, odnosno neke zemlje su uradile veoma malo dok je ogroman broj zemalja koje nisu učinile ništa. Sve ovo se može objasniti na pojavi pandemije COVID 19 koja traje više od dvije godine sa veoma mnogo nejasnih situacija kao što je porijeklo virusa, mutacije, dijagnostika i vakcinacija. Poražavajuća je činjenica da je učešće veterinarskih institucija u periodu pandemije bilo veoma nezapaženo. U nekim zemljama su formirane komisije ili ad-hok radne grupe za zonoze, ali nije bilo sistemskog pristupa po konceptu ”jedno zdravlje”. Cilj ovog rada je da se navedu različita iskustva pojedinih zemalja kod pojava određenih bolesti kao što su COVID 19, bjesnilo i ptičji grip u regionu, zatim iskustva u kontroli antimikrobne rezistencije. Takođe, da se ukaže na prednosti kao i šanse kod primjene koncepta ”jedno zdravlje”, na dobrim i lošim iskustvima na lokalnom, regionalnom i globalnom nivou.
AB  - The causative agents of infectious diseases in humans are over 60% of
animal origin, which classifies these diseases as zoonoses. Therefore,
it is imperative that these diseases be eradicated locally and globally.
The concept of „One Health“ is based on awareness of the need to
protect public health, acting primarily on the prevention and control of
pathogens within human and animal populations in the environment in
which they live. The concept of „One Health“ began to be applied after
the signing of a joint document between the World Health Organization
(WHO), the World Organization for Animal Health (RES) and the
Food and Agriculture Organization of the United Nations (FAO). This
document sets out mutual responsibilities and goals in this area. From
the very beginning, the priority topics for joint action were: the rabies
virus, which kills over 59,000 people a year, and other zoonotic viruses such as bird flu. Antimicrobial resistance is one of the first priorities.
All necessary activities of experts in the field of animal health, public
health and the environment should be implemented at the local,
national and global levels. This should contribute to the continuous
and simultaneous improvement of public health, animal health and
environmental protection worldwide.
The most economical and efficient solution for human protection is
the fight against all zoonotic pathogens in the animal population. This
requires a strategic approach, organization, management and control
with adequate investment of financial resources and engagement of
available human and material resources from both the public and private
sectors. This means establishing new mechanisms for mutual information
and synchronized joint action. Pathogens that are not zoonoses, but
have an indirect negative impact on health, food production and
the environment, should certainly be taken into account. All three
international organizations publish standards and recommendations on
how to act in certain circumstances that are helpful to the competent
decision-making institutions. Despite the fact that the concept of
„One Health“ was established a few decades ago, there is no change
in policy in this regard. Very few countries have adopted a strategy
based on the concept of „One Health“, i.e. some countries have done
very little while a huge number of countries have done nothing. All
this can be explained by the appearance of the COVID 19 pandemic,
which has been going on for more than two years, with many unclear
situations such as the origin of the virus, mutations, diagnostics and
vaccinations. It is a devastating fact that the involvement of veterinary
institutions during the pandemic period was very unnoticed. In some
countries, commissions or ad-hoc working groups on zonoses have been
established, but there has been no systematic approach to the concept
of „One Health“.
The aim of this paper is to list the different experiences of individual
countries in the occurrence of certain diseases such as COVID 19,
rabies and bird flu in the region, as well as experiences in the control
of antimicrobial resistance. Also, to point out the advantages and
opportunities in the application of the concept of „one health“, the
good and bad experiences at the local, regional and global level.
PB  - Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
T1  - Critical analysis of the application of the concept „One health“
T1  - Kritička analiza primjene koncepta „jedno zdravlje“
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2484
ER  - 
@conference{
author = "Nedić, Drago and Petrović, Tamaš and Rodić Vukmir, Nina and Dragana, Dimitrijević and Đurić, Boban and Toplak, Ivan and Laušević, Dejan and Cvetnić, Željko",
year = "2022",
abstract = "Uzročnici infektivnih bolesti ljudi su preko 60% porijeklom od životinja što ove bolesti svrstava u zoonoze. Zbog toga je imperativ da se ove bolesti suzbijaju na lokalnom pa i globalnom nivou. Koncept „jedno zdravlje“ zasnovan je na svijesti o potrebi za zaštitu javnog zdravlja djelujući prevashodno na prevenciji i kontroli patogena unutar humane i životinjske populacija u životnoj sredini u kojoj žive. Koncept ”jedno zdravlje” se počeo primjenjivati nakon potpisivanja zajedničkog dokumenta između Svjetske zdravstvene organizacije (SZO), Svetske organizacije za zdravlje životinja (OIE) i Organizacije za hranu i poljoprivredu Ujedinjenih nacija (FAO). Tim dokumentom su navedene uzajamne odgovornosti i ciljevi u ovoj oblasti. Od samog početka, za prioritetne teme zajedničkog djelovanja postavljeni su: virus bjesnila, od kojeg godišnje umire preko 59.000 ljudi, zatim druge viruse zoonotskog karaktera kao što je ptičji grip. Među prve prioritete uvrštena je i antimikrobna rezistencija. Sve neophodne aktivnosti stručnjaka iz oblasti zdravlja životinja, javnog zdravlja i životne sredine treba da se primjenjuju na lokalnom, nacionalnom i globalnom nivou. To bi trebalo da doprinese stalnom i istovremenom unapređenju javnog zdravlja, zdravlja životinja i zaštite životne sredine širom svijeta. Najekonomičnije i najefikasnije rešenje za zaštitu ljudi je borba protiv svih zoonotskih patogena u životinjskoj populaciji. To zahtijeva strateški pristup, organizovanje, upravljanje i kontrolu uz adekvatna ulaganje finansijskih sredstava i angažovanje raspoloživih ljudskih i materijalnih resursa i iz javnog i privatnog sektora. To podrazimijeva da se uspostave novi mehanizmi međusobnog informisanja i sinhronizovanog zajedničkog djelovanja. Svakako treba voditi računa i o patogenima koji nisu zoonoze, ali imaju indirektni negativan uticaj na zdravlje, proizvodnju hrane i životnu sredinu. Sve tri međunarodne organizacije objavljuju standarde i preporuke o postupanju u određenim okolnostima koje su od pomoći nadležnim institucijama za donošenje odluka. I pored toga što je koncept ”jedno zdravlje” uspostavljen prije par decenija ipak se na terenu ne primjećuje promjena politike u ovom pogledu. Veoma mali broj zemalja je donio strategiju po konceptu ”jedno zdravlje”, odnosno neke zemlje su uradile veoma malo dok je ogroman broj zemalja koje nisu učinile ništa. Sve ovo se može objasniti na pojavi pandemije COVID 19 koja traje više od dvije godine sa veoma mnogo nejasnih situacija kao što je porijeklo virusa, mutacije, dijagnostika i vakcinacija. Poražavajuća je činjenica da je učešće veterinarskih institucija u periodu pandemije bilo veoma nezapaženo. U nekim zemljama su formirane komisije ili ad-hok radne grupe za zonoze, ali nije bilo sistemskog pristupa po konceptu ”jedno zdravlje”. Cilj ovog rada je da se navedu različita iskustva pojedinih zemalja kod pojava određenih bolesti kao što su COVID 19, bjesnilo i ptičji grip u regionu, zatim iskustva u kontroli antimikrobne rezistencije. Takođe, da se ukaže na prednosti kao i šanse kod primjene koncepta ”jedno zdravlje”, na dobrim i lošim iskustvima na lokalnom, regionalnom i globalnom nivou., The causative agents of infectious diseases in humans are over 60% of
animal origin, which classifies these diseases as zoonoses. Therefore,
it is imperative that these diseases be eradicated locally and globally.
The concept of „One Health“ is based on awareness of the need to
protect public health, acting primarily on the prevention and control of
pathogens within human and animal populations in the environment in
which they live. The concept of „One Health“ began to be applied after
the signing of a joint document between the World Health Organization
(WHO), the World Organization for Animal Health (RES) and the
Food and Agriculture Organization of the United Nations (FAO). This
document sets out mutual responsibilities and goals in this area. From
the very beginning, the priority topics for joint action were: the rabies
virus, which kills over 59,000 people a year, and other zoonotic viruses such as bird flu. Antimicrobial resistance is one of the first priorities.
All necessary activities of experts in the field of animal health, public
health and the environment should be implemented at the local,
national and global levels. This should contribute to the continuous
and simultaneous improvement of public health, animal health and
environmental protection worldwide.
The most economical and efficient solution for human protection is
the fight against all zoonotic pathogens in the animal population. This
requires a strategic approach, organization, management and control
with adequate investment of financial resources and engagement of
available human and material resources from both the public and private
sectors. This means establishing new mechanisms for mutual information
and synchronized joint action. Pathogens that are not zoonoses, but
have an indirect negative impact on health, food production and
the environment, should certainly be taken into account. All three
international organizations publish standards and recommendations on
how to act in certain circumstances that are helpful to the competent
decision-making institutions. Despite the fact that the concept of
„One Health“ was established a few decades ago, there is no change
in policy in this regard. Very few countries have adopted a strategy
based on the concept of „One Health“, i.e. some countries have done
very little while a huge number of countries have done nothing. All
this can be explained by the appearance of the COVID 19 pandemic,
which has been going on for more than two years, with many unclear
situations such as the origin of the virus, mutations, diagnostics and
vaccinations. It is a devastating fact that the involvement of veterinary
institutions during the pandemic period was very unnoticed. In some
countries, commissions or ad-hoc working groups on zonoses have been
established, but there has been no systematic approach to the concept
of „One Health“.
The aim of this paper is to list the different experiences of individual
countries in the occurrence of certain diseases such as COVID 19,
rabies and bird flu in the region, as well as experiences in the control
of antimicrobial resistance. Also, to point out the advantages and
opportunities in the application of the concept of „one health“, the
good and bad experiences at the local, regional and global level.",
publisher = "Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)",
title = "Critical analysis of the application of the concept „One health“, Kritička analiza primjene koncepta „jedno zdravlje“",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2484"
}
Nedić, D., Petrović, T., Rodić Vukmir, N., Dragana, D., Đurić, B., Toplak, I., Laušević, D.,& Cvetnić, Ž.. (2022). Critical analysis of the application of the concept „One health“. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
Veterinarska komora Republike Srpske..
https://hdl.handle.net/21.15107/rcub_veterinar_2484
Nedić D, Petrović T, Rodić Vukmir N, Dragana D, Đurić B, Toplak I, Laušević D, Cvetnić Ž. Critical analysis of the application of the concept „One health“. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina). 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2484 .
Nedić, Drago, Petrović, Tamaš, Rodić Vukmir, Nina, Dragana, Dimitrijević, Đurić, Boban, Toplak, Ivan, Laušević, Dejan, Cvetnić, Željko, "Critical analysis of the application of the concept „One health“" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2484 .

West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera

Vasić, Ana; Răileanu, Cristian; Körsten, Christin; Vojinović, Dragica; Manić, Marija; Urošević, Aleksandar; Nikolić, Nataša; Dulović, Olga; Tews, Birke Andrea; Petrović, Tamaš; Silaghi, Cornelia; Valčić, Miroslav

(Wiley, 2022)

TY  - JOUR
AU  - Vasić, Ana
AU  - Răileanu, Cristian
AU  - Körsten, Christin
AU  - Vojinović, Dragica
AU  - Manić, Marija
AU  - Urošević, Aleksandar
AU  - Nikolić, Nataša
AU  - Dulović, Olga
AU  - Tews, Birke Andrea
AU  - Petrović, Tamaš
AU  - Silaghi, Cornelia
AU  - Valčić, Miroslav
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2840
AB  - West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p =.003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
PB  - Wiley
T2  - Transboundary and Emerging Diseases
T1  - West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
VL  - 69
VL  - 5
SP  - e2506
EP  - e2515
DO  - 10.1111/tbed.14593
ER  - 
@article{
author = "Vasić, Ana and Răileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valčić, Miroslav",
year = "2022",
abstract = "West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p =.003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.",
publisher = "Wiley",
journal = "Transboundary and Emerging Diseases",
title = "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera",
volume = "69, 5",
pages = "e2506-e2515",
doi = "10.1111/tbed.14593"
}
Vasić, A., Răileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C.,& Valčić, M.. (2022). West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases
Wiley., 69, e2506-e2515.
https://doi.org/10.1111/tbed.14593
Vasić A, Răileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valčić M. West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases. 2022;69:e2506-e2515.
doi:10.1111/tbed.14593 .
Vasić, Ana, Răileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valčić, Miroslav, "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera" in Transboundary and Emerging Diseases, 69 (2022):e2506-e2515,
https://doi.org/10.1111/tbed.14593 . .
2
1

Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji

Ilić, Tamara; Petrović, Tamaš; Stepanović, Predrag; Bogunović, Danica; Gajić, Bojan; Kulišić, Zoran; Ristić, Marko; Hadži-Milić, Milan; Dimitrijević, Sanda

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2021)

TY  - CONF
AU  - Ilić, Tamara
AU  - Petrović, Tamaš
AU  - Stepanović, Predrag
AU  - Bogunović, Danica
AU  - Gajić, Bojan
AU  - Kulišić, Zoran
AU  - Ristić, Marko
AU  - Hadži-Milić, Milan
AU  - Dimitrijević, Sanda
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3292
AB  - Рад представља ретроспективни извештај и резиме истраживања спроведених по пројекту
ТР31084 у периоду од 2015. – 2020. године. Студија има за циљ да укаже на повећање
преваленције нематода са зоонозним потенцијалом код домаћих и дивљих месоједа на
подручју Србије. Приказана епизоотиолошка ситуација настала је као последица
глобалног загревања и активних миграција власника са својим љубимцима у земље
региона и Европе, од којих многе већ представљају ендемска подручја дијагностикованих
узрочника. Наведени фактори су имали за последицу повећање заступљености појединих
кардиореспираторних, окуларних и интестиналних паразитоза, од којих су неке попримиле
и ендемски карактер на подручју Србије. У популацији испитиваних домаћих месоједа
(власнички пси и мачке, службени пси и пси из прихватилишта) дијагностикована је
највећа преваленција Dirofilaria immitis (68,00%) код службених паса са подручја Београда
и Thelazia callipaeda (47,50%) код власничких паса из Борског округа. Од интестиналних
нематода најзаступљеније су биле Toxocara canis (42,85%) код паса из Прихватилишта у
Земуну и Dipylidium caninum (21,49 - 24,70%) код власничких мачака и паса са територије
Града Београда. Код испитиваних дивљих месоједа (лисице и шакали) доминирало је
присуство респираторне нематоде Capillaria aerophila (74,04%) код лисица из Зајечарског
округа. Токсокароза лисица (84,05%) и анкилостоматидоза шакала (40,90%), биле су
најфреквентније интестиналне нематодозе дивљих месоједа са подручја Републике Србије
обухваћених спроведеним паразитолошким скринингом. Урбанизација градова у Србији
доводи до ширења градског појаса на периферне делове града и викенд насеља, на којима
су до недавно боравиле само лисице. Ширењем градског појаса долази до блиског контакта
паса луталица и власничких дворишних паса са лисицама. Резултат таквог контакта је
значајно мењање паразитофауне паса, тако да они постају извор нових узрочника, које
касније преко свог гастроинтестиналног тракта елиминишу на јавне површине и
представљају извор инфекције за људску популацију. У Србији, као и у многим земљама
Европе, законски је регулисана вакцинација лисица против беснила (путем мамаца).
Захваљујући томе, беснило је стављено под контролу, али се повећао број лисица, а самим
тим и преваленција паразита за које лисице представљају изворе/резервоаре инфекције. То
је само додатни фактор који у ланцу: лисица - пас луталица - власнички пас - човек,
значајно повећава ризик и тенденцију за настанак инфекције људи цревним паразитима
дивљих и домаћих месоједа.
AB  - The paper presents a retrospective report and a summary of the research conducted under the
TR31084 project in the period 2015-2020. The study aims to point to an increase in the
prevalence of nematodes with zoonotic potential in domestic and wild carnivores in Serbia.
The presented epizootiological situation arose as a result of global warming and active
migration of owners with their pets to countries in the region and Europe, many of which
already represent endemic areas of diagnosed pathogens. These factors have led to an increase
in the prevalence of certain cardiorespiratory, ocular and intestinal parasitoses, some of which
have also become endemic in Serbia. The population of domestic carnivores surveyed (owner
dogs and cats, service dogs and shelter dogs) was diagnosed with the highest prevalence of
Dirofilaria immitis (68.00%) in service dogs from Belgrade and Thelazia callipaeda (47.50%)
in owner dogs from Bor district. Of the intestinal nematodes, the most frequent were Toxocara
canis (42.85%) in dogs from the shelter in Zemun and Dipylidium caninum (21.49 - 24.70%)
in owner cats and dogs from the territory of the City of Belgrade. In the wild carnivores (foxes
and jackals) examined, the presence of the respiratory nematode Capillaria aerophila
(74.04%) was dominated by foxes from the Zaječar district. Toxarosis of foxes (84.05%) and
ancylostomatidosis of jackals (40.90%) were the most frequent intestinal nematodes of wild
carnivores in the Republic of Serbia, covered by parasitological screening. Urbanization of
cities in Serbia has led to the expansion of the urban belt to the peripheral parts of the city and
to the cottage settlements, which until recently were occupied only by foxes. With the
widening of the city belt, close contact of stray dogs and owner's backyard dogs is handcuffed.
The result of such contact is a significant alteration of the parasitofauna of dogs, so that they
become a source of new pathogens, which later through their gastrointestinal tract eliminate to
public areas and are a source of infection for the human population. In Serbia, as in many
European countries, vaccination of foxes against rabies (through bait) is legally regulated. As
a result, rabies has been brought under control, but the number of foxes has increased, and
therefore the prevalence of parasites for which foxes are sources / reservoirs of infection. This
is just an additional factor in the chain: fox - stray dog - owner dog - human, significantly
increases the risk and the tendency for humans to become infected with intestinal parasites of
wild and domestic carnivores.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
T1  - Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji
T1  - Epizootiological situation of more important zoonotic helminthoses of domestic and wild carnivores in Serbia
SP  - 40
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3292
ER  - 
@conference{
author = "Ilić, Tamara and Petrović, Tamaš and Stepanović, Predrag and Bogunović, Danica and Gajić, Bojan and Kulišić, Zoran and Ristić, Marko and Hadži-Milić, Milan and Dimitrijević, Sanda",
year = "2021",
abstract = "Рад представља ретроспективни извештај и резиме истраживања спроведених по пројекту
ТР31084 у периоду од 2015. – 2020. године. Студија има за циљ да укаже на повећање
преваленције нематода са зоонозним потенцијалом код домаћих и дивљих месоједа на
подручју Србије. Приказана епизоотиолошка ситуација настала је као последица
глобалног загревања и активних миграција власника са својим љубимцима у земље
региона и Европе, од којих многе већ представљају ендемска подручја дијагностикованих
узрочника. Наведени фактори су имали за последицу повећање заступљености појединих
кардиореспираторних, окуларних и интестиналних паразитоза, од којих су неке попримиле
и ендемски карактер на подручју Србије. У популацији испитиваних домаћих месоједа
(власнички пси и мачке, службени пси и пси из прихватилишта) дијагностикована је
највећа преваленција Dirofilaria immitis (68,00%) код службених паса са подручја Београда
и Thelazia callipaeda (47,50%) код власничких паса из Борског округа. Од интестиналних
нематода најзаступљеније су биле Toxocara canis (42,85%) код паса из Прихватилишта у
Земуну и Dipylidium caninum (21,49 - 24,70%) код власничких мачака и паса са територије
Града Београда. Код испитиваних дивљих месоједа (лисице и шакали) доминирало је
присуство респираторне нематоде Capillaria aerophila (74,04%) код лисица из Зајечарског
округа. Токсокароза лисица (84,05%) и анкилостоматидоза шакала (40,90%), биле су
најфреквентније интестиналне нематодозе дивљих месоједа са подручја Републике Србије
обухваћених спроведеним паразитолошким скринингом. Урбанизација градова у Србији
доводи до ширења градског појаса на периферне делове града и викенд насеља, на којима
су до недавно боравиле само лисице. Ширењем градског појаса долази до блиског контакта
паса луталица и власничких дворишних паса са лисицама. Резултат таквог контакта је
значајно мењање паразитофауне паса, тако да они постају извор нових узрочника, које
касније преко свог гастроинтестиналног тракта елиминишу на јавне површине и
представљају извор инфекције за људску популацију. У Србији, као и у многим земљама
Европе, законски је регулисана вакцинација лисица против беснила (путем мамаца).
Захваљујући томе, беснило је стављено под контролу, али се повећао број лисица, а самим
тим и преваленција паразита за које лисице представљају изворе/резервоаре инфекције. То
је само додатни фактор који у ланцу: лисица - пас луталица - власнички пас - човек,
значајно повећава ризик и тенденцију за настанак инфекције људи цревним паразитима
дивљих и домаћих месоједа., The paper presents a retrospective report and a summary of the research conducted under the
TR31084 project in the period 2015-2020. The study aims to point to an increase in the
prevalence of nematodes with zoonotic potential in domestic and wild carnivores in Serbia.
The presented epizootiological situation arose as a result of global warming and active
migration of owners with their pets to countries in the region and Europe, many of which
already represent endemic areas of diagnosed pathogens. These factors have led to an increase
in the prevalence of certain cardiorespiratory, ocular and intestinal parasitoses, some of which
have also become endemic in Serbia. The population of domestic carnivores surveyed (owner
dogs and cats, service dogs and shelter dogs) was diagnosed with the highest prevalence of
Dirofilaria immitis (68.00%) in service dogs from Belgrade and Thelazia callipaeda (47.50%)
in owner dogs from Bor district. Of the intestinal nematodes, the most frequent were Toxocara
canis (42.85%) in dogs from the shelter in Zemun and Dipylidium caninum (21.49 - 24.70%)
in owner cats and dogs from the territory of the City of Belgrade. In the wild carnivores (foxes
and jackals) examined, the presence of the respiratory nematode Capillaria aerophila
(74.04%) was dominated by foxes from the Zaječar district. Toxarosis of foxes (84.05%) and
ancylostomatidosis of jackals (40.90%) were the most frequent intestinal nematodes of wild
carnivores in the Republic of Serbia, covered by parasitological screening. Urbanization of
cities in Serbia has led to the expansion of the urban belt to the peripheral parts of the city and
to the cottage settlements, which until recently were occupied only by foxes. With the
widening of the city belt, close contact of stray dogs and owner's backyard dogs is handcuffed.
The result of such contact is a significant alteration of the parasitofauna of dogs, so that they
become a source of new pathogens, which later through their gastrointestinal tract eliminate to
public areas and are a source of infection for the human population. In Serbia, as in many
European countries, vaccination of foxes against rabies (through bait) is legally regulated. As
a result, rabies has been brought under control, but the number of foxes has increased, and
therefore the prevalence of parasites for which foxes are sources / reservoirs of infection. This
is just an additional factor in the chain: fox - stray dog - owner dog - human, significantly
increases the risk and the tendency for humans to become infected with intestinal parasites of
wild and domestic carnivores.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021",
title = "Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji, Epizootiological situation of more important zoonotic helminthoses of domestic and wild carnivores in Serbia",
pages = "40-41",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3292"
}
Ilić, T., Petrović, T., Stepanović, P., Bogunović, D., Gajić, B., Kulišić, Z., Ristić, M., Hadži-Milić, M.,& Dimitrijević, S.. (2021). Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 40-41.
https://hdl.handle.net/21.15107/rcub_veterinar_3292
Ilić T, Petrović T, Stepanović P, Bogunović D, Gajić B, Kulišić Z, Ristić M, Hadži-Milić M, Dimitrijević S. Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021. 2021;:40-41.
https://hdl.handle.net/21.15107/rcub_veterinar_3292 .
Ilić, Tamara, Petrović, Tamaš, Stepanović, Predrag, Bogunović, Danica, Gajić, Bojan, Kulišić, Zoran, Ristić, Marko, Hadži-Milić, Milan, Dimitrijević, Sanda, "Epizootiološka situacija važnijih zoonoznih helmintoza domaćih i divljih mesojeda u Srbiji" in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021 (2021):40-41,
https://hdl.handle.net/21.15107/rcub_veterinar_3292 .

African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia

Nešković, Milijana; Ristić, Bojan; Došenović, Rade; Grubač, Siniša; Petrović, Tamaš; Prodanov-Radulović, Jasna; Polaček, Vladimir

(2021)

TY  - JOUR
AU  - Nešković, Milijana
AU  - Ristić, Bojan
AU  - Došenović, Rade
AU  - Grubač, Siniša
AU  - Petrović, Tamaš
AU  - Prodanov-Radulović, Jasna
AU  - Polaček, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2210
AB  - The fi rst confi rmed case of African Swine Fever (ASF) in Serbia occurred in 2019. Since then, numerous outbreaks in domestic pigs and wild boars have been reported. Until April 2021, all the detected ASF cases were in backyard pigs. Beside backyard and smallholders farming systems as a dominant pig production system in Serbia, large commercial pig farms can also be found, located mostly in villages. In the beginning of April 2021, a large commercial farrow-to-fi nish pig farm in Serbia with almost 19,000 animals was affected with the ASF virus. The pig farm analysed in this paper is located in an area where ASF was confi rmed earlier in both backyard and wild boars. In this study, we describe the overall epidemiological course of the ASF outbreak. Epidemiological investigation using a hypothesis-based approach was conducted in order to reconstruct the disease course until the offi cial notifi cation. All the available data from the farm were analysed with the aim to determine the high-risk period (HRP). Some of the key points to consider when it comes to the sources of infection and entry route of ASF are the following: contamination of the area in the immediate vicinity of the farm, risky human activities, irregularities and some omissions in the external farm biosecurity and immediate proximity of the city waste-yard where the communal waste is disposed of. It was concluded that when commercial pig farms are surrounded by villages with a large number of backyards, hence the anthropogenic factor is the key risk factor for ASF spreading
AB  - Prvi slučaj afričke kuge svinja (AKS) u Srbiji je dijagnostikovan 2019. godine. Od tada, beleži se pojava bolesti u populaciji domać ih i divljih svinja. Sve do aprila 2021. godine žarišta AKS su bila lokalizovana u populaciji domaćih svinja u seoskim dvorištima. Pored seoskih gazdinstava i porodičnih farmi kao dominantnog načina proizvodnje, u Srbiji se nalaze i velike komercijalne farme svinja, uglavnom oko seoskih naselja. Početkom aprila 2021. godine, u komercijalnoj farma svinja, proizvodnog kapaciteta oko 19000 jedinki, potvrdjena je infekcija virusom AKS. U radu je sa epizootiološkog aspekta, hronološki opisan tok infekcije AKS. Epizootiološko istraživanje je zasnovano na hipotezama u cilju utvrdjivanja potencijalnih puteva unošenja AKS. Analizirani su dostupni farmski podaci kako bi se utvrdio vremenski period visokog rizika (HRP). Farma svinja se nalazi u području gde je u prethodnom periodu potvrđena AKS u seoskim gazdinstvima, kao i u populaciji divljih svinja. Kontaminacija ambijenta u neposrednoj blizini farme, visoko rizične aktivnosti humane populacije u okruženju (brojna seoska gazdinstva), postojanje pojedinih aktivnosti visokog rizika u realizaciji eksterne biosigurnosti i neposredna blizina gradske deponije gde se odlaže komunalni otpad, su svakako ključni momenti kada se razmatraju izvori infekcije i put unošenja AKS. Zaključeno je da u uslovima kada su komercijalne farme okružene selima, u kojima su dominira uzgoj domaćih svinja u seoskim dvorištima, ljudske aktivnosti predstavljaju ključni faktor rizika za širenje AKS.
T2  - Acta Veterinaria-Beograd
T2  - Acta Veterinaria-Beograd
T1  - African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia
T1  - Istraživanje pojave afričke kuge svinja na velikoj komercijalnoj farmi svinja u Srbiji
VL  - 71
IS  - 2
SP  - 219
EP  - 229
DO  - 10.2478/acve-2021-0019
ER  - 
@article{
author = "Nešković, Milijana and Ristić, Bojan and Došenović, Rade and Grubač, Siniša and Petrović, Tamaš and Prodanov-Radulović, Jasna and Polaček, Vladimir",
year = "2021",
abstract = "The fi rst confi rmed case of African Swine Fever (ASF) in Serbia occurred in 2019. Since then, numerous outbreaks in domestic pigs and wild boars have been reported. Until April 2021, all the detected ASF cases were in backyard pigs. Beside backyard and smallholders farming systems as a dominant pig production system in Serbia, large commercial pig farms can also be found, located mostly in villages. In the beginning of April 2021, a large commercial farrow-to-fi nish pig farm in Serbia with almost 19,000 animals was affected with the ASF virus. The pig farm analysed in this paper is located in an area where ASF was confi rmed earlier in both backyard and wild boars. In this study, we describe the overall epidemiological course of the ASF outbreak. Epidemiological investigation using a hypothesis-based approach was conducted in order to reconstruct the disease course until the offi cial notifi cation. All the available data from the farm were analysed with the aim to determine the high-risk period (HRP). Some of the key points to consider when it comes to the sources of infection and entry route of ASF are the following: contamination of the area in the immediate vicinity of the farm, risky human activities, irregularities and some omissions in the external farm biosecurity and immediate proximity of the city waste-yard where the communal waste is disposed of. It was concluded that when commercial pig farms are surrounded by villages with a large number of backyards, hence the anthropogenic factor is the key risk factor for ASF spreading, Prvi slučaj afričke kuge svinja (AKS) u Srbiji je dijagnostikovan 2019. godine. Od tada, beleži se pojava bolesti u populaciji domać ih i divljih svinja. Sve do aprila 2021. godine žarišta AKS su bila lokalizovana u populaciji domaćih svinja u seoskim dvorištima. Pored seoskih gazdinstava i porodičnih farmi kao dominantnog načina proizvodnje, u Srbiji se nalaze i velike komercijalne farme svinja, uglavnom oko seoskih naselja. Početkom aprila 2021. godine, u komercijalnoj farma svinja, proizvodnog kapaciteta oko 19000 jedinki, potvrdjena je infekcija virusom AKS. U radu je sa epizootiološkog aspekta, hronološki opisan tok infekcije AKS. Epizootiološko istraživanje je zasnovano na hipotezama u cilju utvrdjivanja potencijalnih puteva unošenja AKS. Analizirani su dostupni farmski podaci kako bi se utvrdio vremenski period visokog rizika (HRP). Farma svinja se nalazi u području gde je u prethodnom periodu potvrđena AKS u seoskim gazdinstvima, kao i u populaciji divljih svinja. Kontaminacija ambijenta u neposrednoj blizini farme, visoko rizične aktivnosti humane populacije u okruženju (brojna seoska gazdinstva), postojanje pojedinih aktivnosti visokog rizika u realizaciji eksterne biosigurnosti i neposredna blizina gradske deponije gde se odlaže komunalni otpad, su svakako ključni momenti kada se razmatraju izvori infekcije i put unošenja AKS. Zaključeno je da u uslovima kada su komercijalne farme okružene selima, u kojima su dominira uzgoj domaćih svinja u seoskim dvorištima, ljudske aktivnosti predstavljaju ključni faktor rizika za širenje AKS.",
journal = "Acta Veterinaria-Beograd, Acta Veterinaria-Beograd",
title = "African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia, Istraživanje pojave afričke kuge svinja na velikoj komercijalnoj farmi svinja u Srbiji",
volume = "71",
number = "2",
pages = "219-229",
doi = "10.2478/acve-2021-0019"
}
Nešković, M., Ristić, B., Došenović, R., Grubač, S., Petrović, T., Prodanov-Radulović, J.,& Polaček, V.. (2021). African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia. in Acta Veterinaria-Beograd, 71(2), 219-229.
https://doi.org/10.2478/acve-2021-0019
Nešković M, Ristić B, Došenović R, Grubač S, Petrović T, Prodanov-Radulović J, Polaček V. African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia. in Acta Veterinaria-Beograd. 2021;71(2):219-229.
doi:10.2478/acve-2021-0019 .
Nešković, Milijana, Ristić, Bojan, Došenović, Rade, Grubač, Siniša, Petrović, Tamaš, Prodanov-Radulović, Jasna, Polaček, Vladimir, "African Swine Fever Outbreak Investigation on Large Commercial Pig Farm in Serbia" in Acta Veterinaria-Beograd, 71, no. 2 (2021):219-229,
https://doi.org/10.2478/acve-2021-0019 . .
10
9

Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia

Ilić, Tamara; Petrović, Tamaš; Stepanović, Predrag; Bogunović, Danica; Gajić, Bojan; Kulišić, Zoran; Ristić, Marko; Hadži-Milić, Milan; Dimitrijević, Sanda

(Novi Sad : Scientific Institute for Veterinary Medicine, 2020)

TY  - JOUR
AU  - Ilić, Tamara
AU  - Petrović, Tamaš
AU  - Stepanović, Predrag
AU  - Bogunović, Danica
AU  - Gajić, Bojan
AU  - Kulišić, Zoran
AU  - Ristić, Marko
AU  - Hadži-Milić, Milan
AU  - Dimitrijević, Sanda
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3293
AB  - In the last decade, as the result of climate changes, there have been considerable
changes in the parasitofauna of domestic and wild carnivores. Th e
prevalence of the existing parasitic species has varied signifi cantly, showing
an increasing tendency, and some parasitic species not present before
in this epizootiologic territory have been diagnosed as well. It is thought
that the reason for such an epizootiological situation is increased presence
of owners with their pet animals in the regions endemic for particular
zoonotic helminthoses during summer holidays and touristic visits. Th is
tendency has become especially conspicuous in the last several years characterized
by warm winters and very hot summers due to global warming
eff ects, with abundant atmospheric precipitation. Oral vaccination of foxes
against rabies, regulated in Serbia by appropriate laws since 2010, has led to
an increased number of foxes and rise of prevalence of the parasitic diseases for which foxes represent the infection source/reservoir. Continued urbanization
of Serbian cities, with the extension of urban belts into the suburbia
and recreational (“weekend”) settlements, lead to a closer contact of street
dogs and owned dogs with foxes, which results in a signifi cant change in
the parasitic fauna of dogs. It is an additional factor, which in the chain
fox - street dog - owned dog - human increases the risk and tendency for
the occurrence of human infections with zoonotic endoparasites of wild
and domestic carnivores. In order to reliably predict the degree of spread of
particular zoonotic helminthoses in particular regions in Serbia, for which
wild carnivores represent the infection source, it is necessary to institute
continued monitoring of the parasitic fauna in this type of wild animals.
PB  - Novi Sad : Scientific Institute for Veterinary Medicine
T2  - Arhiv veterinarske medicine
T1  - Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia
VL  - 13
IS  - 1
SP  - 5
EP  - 28
DO  - 10.46784/e-avm.v13i1.238
ER  - 
@article{
author = "Ilić, Tamara and Petrović, Tamaš and Stepanović, Predrag and Bogunović, Danica and Gajić, Bojan and Kulišić, Zoran and Ristić, Marko and Hadži-Milić, Milan and Dimitrijević, Sanda",
year = "2020",
abstract = "In the last decade, as the result of climate changes, there have been considerable
changes in the parasitofauna of domestic and wild carnivores. Th e
prevalence of the existing parasitic species has varied signifi cantly, showing
an increasing tendency, and some parasitic species not present before
in this epizootiologic territory have been diagnosed as well. It is thought
that the reason for such an epizootiological situation is increased presence
of owners with their pet animals in the regions endemic for particular
zoonotic helminthoses during summer holidays and touristic visits. Th is
tendency has become especially conspicuous in the last several years characterized
by warm winters and very hot summers due to global warming
eff ects, with abundant atmospheric precipitation. Oral vaccination of foxes
against rabies, regulated in Serbia by appropriate laws since 2010, has led to
an increased number of foxes and rise of prevalence of the parasitic diseases for which foxes represent the infection source/reservoir. Continued urbanization
of Serbian cities, with the extension of urban belts into the suburbia
and recreational (“weekend”) settlements, lead to a closer contact of street
dogs and owned dogs with foxes, which results in a signifi cant change in
the parasitic fauna of dogs. It is an additional factor, which in the chain
fox - street dog - owned dog - human increases the risk and tendency for
the occurrence of human infections with zoonotic endoparasites of wild
and domestic carnivores. In order to reliably predict the degree of spread of
particular zoonotic helminthoses in particular regions in Serbia, for which
wild carnivores represent the infection source, it is necessary to institute
continued monitoring of the parasitic fauna in this type of wild animals.",
publisher = "Novi Sad : Scientific Institute for Veterinary Medicine",
journal = "Arhiv veterinarske medicine",
title = "Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia",
volume = "13",
number = "1",
pages = "5-28",
doi = "10.46784/e-avm.v13i1.238"
}
Ilić, T., Petrović, T., Stepanović, P., Bogunović, D., Gajić, B., Kulišić, Z., Ristić, M., Hadži-Milić, M.,& Dimitrijević, S.. (2020). Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia. in Arhiv veterinarske medicine
Novi Sad : Scientific Institute for Veterinary Medicine., 13(1), 5-28.
https://doi.org/10.46784/e-avm.v13i1.238
Ilić T, Petrović T, Stepanović P, Bogunović D, Gajić B, Kulišić Z, Ristić M, Hadži-Milić M, Dimitrijević S. Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia. in Arhiv veterinarske medicine. 2020;13(1):5-28.
doi:10.46784/e-avm.v13i1.238 .
Ilić, Tamara, Petrović, Tamaš, Stepanović, Predrag, Bogunović, Danica, Gajić, Bojan, Kulišić, Zoran, Ristić, Marko, Hadži-Milić, Milan, Dimitrijević, Sanda, "Zoonotic helminthosis of domestic and wild carnivores in the epizootiologic territory of Serbia" in Arhiv veterinarske medicine, 13, no. 1 (2020):5-28,
https://doi.org/10.46784/e-avm.v13i1.238 . .
1

Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema

Bugarski, Dejan; Petrović, Tamaš; Lupulović, Diana; Lazić, Sava; Todorović, Dalibor; Savić, Sara; Bojkovski, Jovan

(Beograd : Srpsko veterinarsko društvo, 2020)

TY  - CONF
AU  - Bugarski, Dejan
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Lazić, Sava
AU  - Todorović, Dalibor
AU  - Savić, Sara
AU  - Bojkovski, Jovan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3520
AB  - Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
T1  - Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema
SP  - 51
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3520
ER  - 
@conference{
author = "Bugarski, Dejan and Petrović, Tamaš and Lupulović, Diana and Lazić, Sava and Todorović, Dalibor and Savić, Sara and Bojkovski, Jovan",
year = "2020",
abstract = "Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020",
title = "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema",
pages = "51-62",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3520"
}
Bugarski, D., Petrović, T., Lupulović, D., Lazić, S., Todorović, D., Savić, S.,& Bojkovski, J.. (2020). Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
Beograd : Srpsko veterinarsko društvo., 51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520
Bugarski D, Petrović T, Lupulović D, Lazić S, Todorović D, Savić S, Bojkovski J. Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020. 2020;:51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .
Bugarski, Dejan, Petrović, Tamaš, Lupulović, Diana, Lazić, Sava, Todorović, Dalibor, Savić, Sara, Bojkovski, Jovan, "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema" in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020 (2020):51-62,
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

Classical swine fever virus detection in fetal swine tissues by immunohistochemistry

Polaček, Vladimir; Đurđević, Biljana; Petrović, Tamaš; Prodanov-Radulović, Jasna; Samojlović, Milena; Vučićević, Ivana; Aleksić-Kovačević, Sanja

(Scientific Veterinary Institute “Novi Sad”, 2020)

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Đurđević, Biljana
AU  - Petrović, Tamaš
AU  - Prodanov-Radulović, Jasna
AU  - Samojlović, Milena
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1892
AB  - The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis.
AB  - Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.
PB  - Scientific Veterinary Institute “Novi Sad”
T2  - Archives of Veterinary Medicine
T1  - Classical swine fever virus detection in fetal swine tissues by immunohistochemistry
T1  - Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode
VL  - 13
IS  - 1
DO  - 10.46784/e-avm.v13i1.235
ER  - 
@article{
author = "Polaček, Vladimir and Đurđević, Biljana and Petrović, Tamaš and Prodanov-Radulović, Jasna and Samojlović, Milena and Vučićević, Ivana and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis., Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.",
publisher = "Scientific Veterinary Institute “Novi Sad”",
journal = "Archives of Veterinary Medicine",
title = "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry, Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode",
volume = "13",
number = "1",
doi = "10.46784/e-avm.v13i1.235"
}
Polaček, V., Đurđević, B., Petrović, T., Prodanov-Radulović, J., Samojlović, M., Vučićević, I.,& Aleksić-Kovačević, S.. (2020). Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”., 13(1).
https://doi.org/10.46784/e-avm.v13i1.235
Polaček V, Đurđević B, Petrović T, Prodanov-Radulović J, Samojlović M, Vučićević I, Aleksić-Kovačević S. Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine. 2020;13(1).
doi:10.46784/e-avm.v13i1.235 .
Polaček, Vladimir, Đurđević, Biljana, Petrović, Tamaš, Prodanov-Radulović, Jasna, Samojlović, Milena, Vučićević, Ivana, Aleksić-Kovačević, Sanja, "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry" in Archives of Veterinary Medicine, 13, no. 1 (2020),
https://doi.org/10.46784/e-avm.v13i1.235 . .

Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016

Manić, Marija; Stojiljković, Marko; Petrović, Milos; Nišavić, Jakov; Bacić, Dragan; Petrović, Tamaš; Vidanović, Dejan; Obrenović, Sonja

(Wiley, Hoboken, 2019)

TY  - JOUR
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Petrović, Milos
AU  - Nišavić, Jakov
AU  - Bacić, Dragan
AU  - Petrović, Tamaš
AU  - Vidanović, Dejan
AU  - Obrenović, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1792
AB  - Lumpy skin disease (LSD) is an infectious disease of cattle caused by virus of the Capripoxvirus genus (LSDV), family Poxviridae. Until 2015, it had not appeared in the Balkans. In June 2016, LSD spread throughout Serbia. This study analyses the first LSD occurrence, epizootic features, applied diagnostic procedures and control measures in five districts in south-east Serbia (Pcinja, Jablanica, Pirot, Toplica and Nisava). In total, there were 225 LSD outbreaks reported in Serbia, out of which 189 (84%) were located in the study area. The highest number of outbreaks was registered in Pcinja district (169), where LSD was first registered. The median distance and time between the nearest previous outbreak sites were calculated (4.32 km and 9 days). The median altitude of outbreak locations was 992 m with more than 90% above 500 m (p <= 0.001). The average herd morbidity rate in the study area was 13.6% and the herd mortality rate was recorded only in Pcinja (0.5%) and Jablanica (1.6%) districts. Samples taken from the cattle suspected to LSD were subjected to real time PCR analysis. Out of 233 samples tested for LSDV 132 (56.7%) were positive. The LSDV genome was identified in skin nodules (85.4%), blood (72.7%) and nasal swabs (62.5%). Phylogenetic analysis indicated that the LSDV strain circulating in Serbia fell within the cluster of field LSDV found worldwide. In response to the LSD epizootic, animal trade and movement were prohibited, complete stamping out, disinfection, disinsection and an entire bovine population vaccination using the homologous Neethling live attenuated vaccine (OBP, South Africa) were conducted. A month and a half after the completion of the vaccination campaign, the LSD epizootic was stopped, and no new cases have been reported since.
PB  - Wiley, Hoboken
T2  - Transboundary and Emerging Diseases
T1  - Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016
VL  - 66
IS  - 5
SP  - 2087
EP  - 2099
DO  - 10.1111/tbed.13261
ER  - 
@article{
author = "Manić, Marija and Stojiljković, Marko and Petrović, Milos and Nišavić, Jakov and Bacić, Dragan and Petrović, Tamaš and Vidanović, Dejan and Obrenović, Sonja",
year = "2019",
abstract = "Lumpy skin disease (LSD) is an infectious disease of cattle caused by virus of the Capripoxvirus genus (LSDV), family Poxviridae. Until 2015, it had not appeared in the Balkans. In June 2016, LSD spread throughout Serbia. This study analyses the first LSD occurrence, epizootic features, applied diagnostic procedures and control measures in five districts in south-east Serbia (Pcinja, Jablanica, Pirot, Toplica and Nisava). In total, there were 225 LSD outbreaks reported in Serbia, out of which 189 (84%) were located in the study area. The highest number of outbreaks was registered in Pcinja district (169), where LSD was first registered. The median distance and time between the nearest previous outbreak sites were calculated (4.32 km and 9 days). The median altitude of outbreak locations was 992 m with more than 90% above 500 m (p <= 0.001). The average herd morbidity rate in the study area was 13.6% and the herd mortality rate was recorded only in Pcinja (0.5%) and Jablanica (1.6%) districts. Samples taken from the cattle suspected to LSD were subjected to real time PCR analysis. Out of 233 samples tested for LSDV 132 (56.7%) were positive. The LSDV genome was identified in skin nodules (85.4%), blood (72.7%) and nasal swabs (62.5%). Phylogenetic analysis indicated that the LSDV strain circulating in Serbia fell within the cluster of field LSDV found worldwide. In response to the LSD epizootic, animal trade and movement were prohibited, complete stamping out, disinfection, disinsection and an entire bovine population vaccination using the homologous Neethling live attenuated vaccine (OBP, South Africa) were conducted. A month and a half after the completion of the vaccination campaign, the LSD epizootic was stopped, and no new cases have been reported since.",
publisher = "Wiley, Hoboken",
journal = "Transboundary and Emerging Diseases",
title = "Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016",
volume = "66",
number = "5",
pages = "2087-2099",
doi = "10.1111/tbed.13261"
}
Manić, M., Stojiljković, M., Petrović, M., Nišavić, J., Bacić, D., Petrović, T., Vidanović, D.,& Obrenović, S.. (2019). Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016. in Transboundary and Emerging Diseases
Wiley, Hoboken., 66(5), 2087-2099.
https://doi.org/10.1111/tbed.13261
Manić M, Stojiljković M, Petrović M, Nišavić J, Bacić D, Petrović T, Vidanović D, Obrenović S. Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016. in Transboundary and Emerging Diseases. 2019;66(5):2087-2099.
doi:10.1111/tbed.13261 .
Manić, Marija, Stojiljković, Marko, Petrović, Milos, Nišavić, Jakov, Bacić, Dragan, Petrović, Tamaš, Vidanović, Dejan, Obrenović, Sonja, "Epizootic features and control measures for lumpy skin disease in south-east Serbia in 2016" in Transboundary and Emerging Diseases, 66, no. 5 (2019):2087-2099,
https://doi.org/10.1111/tbed.13261 . .
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19

Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia

Milojević, Lazar; Velebit, Branko; Teodorović, Vlado; Kirbis, Andrej; Petrović, Tamaš; Karabasil, Nedjeljko; Dimitrijević, Mirjana

(Springer, New York, 2019)

TY  - JOUR
AU  - Milojević, Lazar
AU  - Velebit, Branko
AU  - Teodorović, Vlado
AU  - Kirbis, Andrej
AU  - Petrović, Tamaš
AU  - Karabasil, Nedjeljko
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1766
AB  - Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV amplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia.
PB  - Springer, New York
T2  - Food and Environmental Virology
T1  - Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia
VL  - 11
IS  - 4
SP  - 410
EP  - 419
DO  - 10.1007/s12560-019-09393-1
ER  - 
@article{
author = "Milojević, Lazar and Velebit, Branko and Teodorović, Vlado and Kirbis, Andrej and Petrović, Tamaš and Karabasil, Nedjeljko and Dimitrijević, Mirjana",
year = "2019",
abstract = "Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV amplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia.",
publisher = "Springer, New York",
journal = "Food and Environmental Virology",
title = "Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia",
volume = "11",
number = "4",
pages = "410-419",
doi = "10.1007/s12560-019-09393-1"
}
Milojević, L., Velebit, B., Teodorović, V., Kirbis, A., Petrović, T., Karabasil, N.,& Dimitrijević, M.. (2019). Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia. in Food and Environmental Virology
Springer, New York., 11(4), 410-419.
https://doi.org/10.1007/s12560-019-09393-1
Milojević L, Velebit B, Teodorović V, Kirbis A, Petrović T, Karabasil N, Dimitrijević M. Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia. in Food and Environmental Virology. 2019;11(4):410-419.
doi:10.1007/s12560-019-09393-1 .
Milojević, Lazar, Velebit, Branko, Teodorović, Vlado, Kirbis, Andrej, Petrović, Tamaš, Karabasil, Nedjeljko, Dimitrijević, Mirjana, "Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia" in Food and Environmental Virology, 11, no. 4 (2019):410-419,
https://doi.org/10.1007/s12560-019-09393-1 . .
5
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21

Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia

Bozic (Durdevic), Biljana; Vučićević, Ivana; Polaček, Vladimir; Vasković, Nikola; Petrović, Tamaš; Pajić, Marko; Aleksić-Kovačević, Sanja

(Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo, 2019)

TY  - JOUR
AU  - Bozic (Durdevic), Biljana
AU  - Vučićević, Ivana
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Petrović, Tamaš
AU  - Pajić, Marko
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1770
AB  - The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.
PB  - Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo
T2  - Veterinaria Italiana
T1  - Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia
VL  - 55
IS  - 1
SP  - 95
EP  - 101
DO  - 10.12834/VetIt.1463.7919.2
ER  - 
@article{
author = "Bozic (Durdevic), Biljana and Vučićević, Ivana and Polaček, Vladimir and Vasković, Nikola and Petrović, Tamaš and Pajić, Marko and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "The aim of this study was to compare pathological lesions and viral antigen expression in the organs of mute swans (Cygnus olor) naturally infected with highly pathogenic avian influenza virus subtypes H5N1 and H5N8. The examination was conducted on the carcasses of 22 mute swans which died during the avian influenza outbreaks in Serbia in 2006 and 2016-2017. Avian influenza virus subtype H5N8 isolated from mute swans in 2016-2017 was clustered within the 2.3.4.4 Glade group B. After necropsy, lung, liver, spleen, pancreas, kidney and brain tissues were sampled for histopathology and immunohistochemical examination. Avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most significant gross lesions were necrosis and haemorrhages in the pancreas. Major histological lesions were multifocal necroses in the pancreas, spleen and liver, non-purulent encephalitis, lung congestion and oedema. Immunohistochemical demonstration of HPAIV nucleoprotein in pancreas and brain was strongly consistent with histological lesions in both infected groups. Our findings showed that pancreas was the most affected organ in all examined mute swans. In addition to increased mortality rate, similar pathological findings were detected in mute swans naturally infected with highly pathogenic avian influenza viruses H5N1 and H5N8.",
publisher = "Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo",
journal = "Veterinaria Italiana",
title = "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia",
volume = "55",
number = "1",
pages = "95-101",
doi = "10.12834/VetIt.1463.7919.2"
}
Bozic (Durdevic), B., Vučićević, I., Polaček, V., Vasković, N., Petrović, T., Pajić, M.,& Aleksić-Kovačević, S.. (2019). Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana
Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, Teramo., 55(1), 95-101.
https://doi.org/10.12834/VetIt.1463.7919.2
Bozic (Durdevic) B, Vučićević I, Polaček V, Vasković N, Petrović T, Pajić M, Aleksić-Kovačević S. Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia. in Veterinaria Italiana. 2019;55(1):95-101.
doi:10.12834/VetIt.1463.7919.2 .
Bozic (Durdevic), Biljana, Vučićević, Ivana, Polaček, Vladimir, Vasković, Nikola, Petrović, Tamaš, Pajić, Marko, Aleksić-Kovačević, Sanja, "Comparative pathological findings in mute swans (Cygnus olor) naturally infected with highly pathogenic Avian influenza viruses H5N1 and H5N8 in Serbia" in Veterinaria Italiana, 55, no. 1 (2019):95-101,
https://doi.org/10.12834/VetIt.1463.7919.2 . .
2
2

Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals

Milanov, Dubravka; Petrović, Tamaš; Todorović, Dalibor; Aleksić, Nevenka; Čabarkapa, Ivana

(Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad, 2018)

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Todorović, Dalibor
AU  - Aleksić, Nevenka
AU  - Čabarkapa, Ivana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1642
AB  - Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed.
AB  - Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals
T1  - Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja
VL  - 45
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/FFR1801067M
ER  - 
@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Todorović, Dalibor and Aleksić, Nevenka and Čabarkapa, Ivana",
year = "2018",
abstract = "Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed., Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals, Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja",
volume = "45",
number = "1",
pages = "67-76",
doi = "10.5937/FFR1801067M"
}
Milanov, D., Petrović, T., Todorović, D., Aleksić, N.,& Čabarkapa, I.. (2018). Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(1), 67-76.
https://doi.org/10.5937/FFR1801067M
Milanov D, Petrović T, Todorović D, Aleksić N, Čabarkapa I. Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research. 2018;45(1):67-76.
doi:10.5937/FFR1801067M .
Milanov, Dubravka, Petrović, Tamaš, Todorović, Dalibor, Aleksić, Nevenka, Čabarkapa, Ivana, "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals" in Food and Feed Research, 45, no. 1 (2018):67-76,
https://doi.org/10.5937/FFR1801067M . .
6

Methodology and results of integrated WNV surveillance programmes in Serbia

Petrović, Tamaš; Šekler, Milanko; Petrić, Dusan; Lazić, Sava; Debeljak, Zoran; Vidanović, Dejan; Ignjatović-Čupina, Aleksandra; Lazić, Gospava; Lupulović, Diana; Kolarević, Migo; Plavsić, Budimir

(Public Library Science, San Francisco, 2018)

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 
@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}
Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439
Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .
Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .
1
22
12
25

Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae

Glavinić, Uroš; Stanković, Biljana; Drašković, Vladimir; Stevanović, Jevrosima; Petrović, Tamaš; Lakić, Nada; Stanimirović, Zoran

(Public Library Science, San Francisco, 2017)

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stanković, Biljana
AU  - Drašković, Vladimir
AU  - Stevanović, Jevrosima
AU  - Petrović, Tamaš
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1541
AB  - Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex BEEWELL AminoPlus to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae
VL  - 12
IS  - 11
SP  - e0187726
DO  - 10.1371/journal.pone.0187726
ER  - 
@article{
author = "Glavinić, Uroš and Stanković, Biljana and Drašković, Vladimir and Stevanović, Jevrosima and Petrović, Tamaš and Lakić, Nada and Stanimirović, Zoran",
year = "2017",
abstract = "Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex BEEWELL AminoPlus to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae",
volume = "12",
number = "11",
pages = "e0187726",
doi = "10.1371/journal.pone.0187726"
}
Glavinić, U., Stanković, B., Drašković, V., Stevanović, J., Petrović, T., Lakić, N.,& Stanimirović, Z.. (2017). Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One
Public Library Science, San Francisco., 12(11), e0187726.
https://doi.org/10.1371/journal.pone.0187726
Glavinić U, Stanković B, Drašković V, Stevanović J, Petrović T, Lakić N, Stanimirović Z. Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One. 2017;12(11):e0187726.
doi:10.1371/journal.pone.0187726 .
Glavinić, Uroš, Stanković, Biljana, Drašković, Vladimir, Stevanović, Jevrosima, Petrović, Tamaš, Lakić, Nada, Stanimirović, Zoran, "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae" in PLoS One, 12, no. 11 (2017):e0187726,
https://doi.org/10.1371/journal.pone.0187726 . .
76
32
72

Vanadium in poultry nutrition

Živkov-Baloš, Milica; Ljubojević, Dragana; Jakšić, Sandra; Mihaljev, Željko; Pelić, Miloš; Petrović, Tamaš; Šefer, Dragan

(Naučni institut za veterinarstvo "Novi Sad", Novi Sad, 2017)

TY  - JOUR
AU  - Živkov-Baloš, Milica
AU  - Ljubojević, Dragana
AU  - Jakšić, Sandra
AU  - Mihaljev, Željko
AU  - Pelić, Miloš
AU  - Petrović, Tamaš
AU  - Šefer, Dragan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1461
AB  - Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt  10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples were above the minimum recommended levels for poultry feed, still not exceeding the maximum tolerable values.
AB  - Vanadijum (V) je esencijalni element u ishrani živine. Relativno niski nivoi V ( lt  10 mg) smanjuju porast pilića i vrednosti Haugh-ovih jedinica jaja. The National Research Council (NRC) preporučuje veoma niske nivoe V u hrani za živinu, pri čemu je za maksimalni nivo tolerancije utvrđena vrednost od 10 mg/kg. Višak vanadijuma u ishrani živine ispoljava štetne efekte u proizvodnji jaja, negativno utiče na unutrašnji kvalitet jaja, telesnu masu živine i efikasnost iskorišćavanja hrane. Podaci o sadržaju V u hrani i hranivima za životinje su oskudni, ali zna se da fosfatna mineralna hraniva često sadrže visoke koncentracije ovog elementa. Cilj ovog istraživanja je bio da se dobiju informacije o sadržaju vanadijuma u hranivima i hrani za živinu. Uzorci hrane za životinje su pripremljeni mikrotalasnom digestijom, a sadržaj V je određen metodom indukovano kuplovane plazme sa masenom spektrometrijom (ICP-MS). Koncentracije vanadijuma u ispitivanim uzorcima bile su iznad minimalnih preporučenih nivoa u ishrani živine, ali nisu prelazile maksimalne tolerantne vrednosti za živinu za ovaj element.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Vanadium in poultry nutrition
T1  - Vanadijum u ishrani živine
VL  - 10
IS  - 1
SP  - 85
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1461
ER  - 
@article{
author = "Živkov-Baloš, Milica and Ljubojević, Dragana and Jakšić, Sandra and Mihaljev, Željko and Pelić, Miloš and Petrović, Tamaš and Šefer, Dragan",
year = "2017",
abstract = "Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt  10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples were above the minimum recommended levels for poultry feed, still not exceeding the maximum tolerable values., Vanadijum (V) je esencijalni element u ishrani živine. Relativno niski nivoi V ( lt  10 mg) smanjuju porast pilića i vrednosti Haugh-ovih jedinica jaja. The National Research Council (NRC) preporučuje veoma niske nivoe V u hrani za živinu, pri čemu je za maksimalni nivo tolerancije utvrđena vrednost od 10 mg/kg. Višak vanadijuma u ishrani živine ispoljava štetne efekte u proizvodnji jaja, negativno utiče na unutrašnji kvalitet jaja, telesnu masu živine i efikasnost iskorišćavanja hrane. Podaci o sadržaju V u hrani i hranivima za životinje su oskudni, ali zna se da fosfatna mineralna hraniva često sadrže visoke koncentracije ovog elementa. Cilj ovog istraživanja je bio da se dobiju informacije o sadržaju vanadijuma u hranivima i hrani za živinu. Uzorci hrane za životinje su pripremljeni mikrotalasnom digestijom, a sadržaj V je određen metodom indukovano kuplovane plazme sa masenom spektrometrijom (ICP-MS). Koncentracije vanadijuma u ispitivanim uzorcima bile su iznad minimalnih preporučenih nivoa u ishrani živine, ali nisu prelazile maksimalne tolerantne vrednosti za živinu za ovaj element.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Vanadium in poultry nutrition, Vanadijum u ishrani živine",
volume = "10",
number = "1",
pages = "85-92",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1461"
}
Živkov-Baloš, M., Ljubojević, D., Jakšić, S., Mihaljev, Ž., Pelić, M., Petrović, T.,& Šefer, D.. (2017). Vanadium in poultry nutrition. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 10(1), 85-92.
https://hdl.handle.net/21.15107/rcub_veterinar_1461
Živkov-Baloš M, Ljubojević D, Jakšić S, Mihaljev Ž, Pelić M, Petrović T, Šefer D. Vanadium in poultry nutrition. in Arhiv veterinarske medicine. 2017;10(1):85-92.
https://hdl.handle.net/21.15107/rcub_veterinar_1461 .
Živkov-Baloš, Milica, Ljubojević, Dragana, Jakšić, Sandra, Mihaljev, Željko, Pelić, Miloš, Petrović, Tamaš, Šefer, Dragan, "Vanadium in poultry nutrition" in Arhiv veterinarske medicine, 10, no. 1 (2017):85-92,
https://hdl.handle.net/21.15107/rcub_veterinar_1461 .

Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia

Veljović, Ljubiša; Knežević, Aleksandra; Milić, Nenad; Krnjaić, Dejan; Miković, Radoš; Zorić, Andrea; Marković, Maja; Milićević, Vesna; Stamenković, Miodrag; Stanojević, Maja; Maksimović-Zorić, Jelena; Petrović, Tamaš; Nišavić, Jakov

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2016)

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Miković, Radoš
AU  - Zorić, Andrea
AU  - Marković, Maja
AU  - Milićević, Vesna
AU  - Stamenković, Miodrag
AU  - Stanojević, Maja
AU  - Maksimović-Zorić, Jelena
AU  - Petrović, Tamaš
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1368
AB  - The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
AB  - Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia
T1  - Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji
VL  - 66
IS  - 4
SP  - 509
EP  - 519
DO  - 10.1515/acve-2016-0044
ER  - 
@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Miković, Radoš and Zorić, Andrea and Marković, Maja and Milićević, Vesna and Stamenković, Miodrag and Stanojević, Maja and Maksimović-Zorić, Jelena and Petrović, Tamaš and Nišavić, Jakov",
year = "2016",
abstract = "The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank., Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia, Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji",
volume = "66",
number = "4",
pages = "509-519",
doi = "10.1515/acve-2016-0044"
}
Veljović, L., Knežević, A., Milić, N., Krnjaić, D., Miković, R., Zorić, A., Marković, M., Milićević, V., Stamenković, M., Stanojević, M., Maksimović-Zorić, J., Petrović, T.,& Nišavić, J.. (2016). Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 509-519.
https://doi.org/10.1515/acve-2016-0044
Veljović L, Knežević A, Milić N, Krnjaić D, Miković R, Zorić A, Marković M, Milićević V, Stamenković M, Stanojević M, Maksimović-Zorić J, Petrović T, Nišavić J. Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd. 2016;66(4):509-519.
doi:10.1515/acve-2016-0044 .
Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Miković, Radoš, Zorić, Andrea, Marković, Maja, Milićević, Vesna, Stamenković, Miodrag, Stanojević, Maja, Maksimović-Zorić, Jelena, Petrović, Tamaš, Nišavić, Jakov, "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia" in Acta Veterinaria-Beograd, 66, no. 4 (2016):509-519,
https://doi.org/10.1515/acve-2016-0044 . .
6
2
6

Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia

Ilić, Tamara; Becskei, Zsolt; Petrović, Tamaš; Polaček, Vladimir; Ristić, Bojan; Milić, Sinisa; Stepanović, Predrag; Radisavljević, Katarina; Dimitrijević, Sanda

(Springer International Publishing Ag, Cham, 2016)

TY  - JOUR
AU  - Ilić, Tamara
AU  - Becskei, Zsolt
AU  - Petrović, Tamaš
AU  - Polaček, Vladimir
AU  - Ristić, Bojan
AU  - Milić, Sinisa
AU  - Stepanović, Predrag
AU  - Radisavljević, Katarina
AU  - Dimitrijević, Sanda
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1395
AB  - Wild canides have a high epizootiological - epidemiological significance, considering that they are hosts for some parasites which spread vector born diseases. Increased frequency of certain interactions between domestic and wild canides increases the risk of occurrence, spreading and maintaining the infection of parasitic etiology in domestic canides. The research was conducted in 232 wild canides (172 red foxes and 60 golden jackals). The examined material was sampled from foxes and jackals, which were hunted down between 2010 and 2014, from 8 epizootiological areas of Serbia (North-Backa, West-Backa, Southern-Banat, Moravicki, Zlatiborski, Raski, Rasinski and Zajecarski district). On completing the parasitological dissection and the coprological diagnostics, in wild canides protozoa from the genus Isospora were identified, 3 species of trematoda (Alaria alata, Pseudamphistomum truncatum and Metagonimus yokogawai), cestods from the genus Taenia and 5 species of nematodes (Toxocara canis, Ancylostomatidae, Trichuris vulpis and Capillaria aerophila). The finding of M. yokogawai in golden jackals were, to the best of our knowledge, one of the first diagnosed cases of metagonimosis in golden jackals in Serbia. The continued monitoring of the parasitic fauna of wild canides is needed to establish the widespread of the zoonoses in different regions of Serbia, because they present the reservoirs and/or sources of these infections.
PB  - Springer International Publishing Ag, Cham
T2  - Acta Parasitologica
T1  - Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia
VL  - 61
IS  - 2
SP  - 389
EP  - 396
DO  - 10.1515/ap-2016-0051
ER  - 
@article{
author = "Ilić, Tamara and Becskei, Zsolt and Petrović, Tamaš and Polaček, Vladimir and Ristić, Bojan and Milić, Sinisa and Stepanović, Predrag and Radisavljević, Katarina and Dimitrijević, Sanda",
year = "2016",
abstract = "Wild canides have a high epizootiological - epidemiological significance, considering that they are hosts for some parasites which spread vector born diseases. Increased frequency of certain interactions between domestic and wild canides increases the risk of occurrence, spreading and maintaining the infection of parasitic etiology in domestic canides. The research was conducted in 232 wild canides (172 red foxes and 60 golden jackals). The examined material was sampled from foxes and jackals, which were hunted down between 2010 and 2014, from 8 epizootiological areas of Serbia (North-Backa, West-Backa, Southern-Banat, Moravicki, Zlatiborski, Raski, Rasinski and Zajecarski district). On completing the parasitological dissection and the coprological diagnostics, in wild canides protozoa from the genus Isospora were identified, 3 species of trematoda (Alaria alata, Pseudamphistomum truncatum and Metagonimus yokogawai), cestods from the genus Taenia and 5 species of nematodes (Toxocara canis, Ancylostomatidae, Trichuris vulpis and Capillaria aerophila). The finding of M. yokogawai in golden jackals were, to the best of our knowledge, one of the first diagnosed cases of metagonimosis in golden jackals in Serbia. The continued monitoring of the parasitic fauna of wild canides is needed to establish the widespread of the zoonoses in different regions of Serbia, because they present the reservoirs and/or sources of these infections.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Acta Parasitologica",
title = "Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia",
volume = "61",
number = "2",
pages = "389-396",
doi = "10.1515/ap-2016-0051"
}
Ilić, T., Becskei, Z., Petrović, T., Polaček, V., Ristić, B., Milić, S., Stepanović, P., Radisavljević, K.,& Dimitrijević, S.. (2016). Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia. in Acta Parasitologica
Springer International Publishing Ag, Cham., 61(2), 389-396.
https://doi.org/10.1515/ap-2016-0051
Ilić T, Becskei Z, Petrović T, Polaček V, Ristić B, Milić S, Stepanović P, Radisavljević K, Dimitrijević S. Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia. in Acta Parasitologica. 2016;61(2):389-396.
doi:10.1515/ap-2016-0051 .
Ilić, Tamara, Becskei, Zsolt, Petrović, Tamaš, Polaček, Vladimir, Ristić, Bojan, Milić, Sinisa, Stepanović, Predrag, Radisavljević, Katarina, Dimitrijević, Sanda, "Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia" in Acta Parasitologica, 61, no. 2 (2016):389-396,
https://doi.org/10.1515/ap-2016-0051 . .
22
12
19

Hypodermosis in northern Serbia (Vojvodina)

Becskei, Zsolt; Ilić, Tamara; Pavlicević, Natasa; Kiskároly, Ferenc; Petrović, Tamaš; Dimitrijević, Sanda

(Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje, 2016)

TY  - JOUR
AU  - Becskei, Zsolt
AU  - Ilić, Tamara
AU  - Pavlicević, Natasa
AU  - Kiskároly, Ferenc
AU  - Petrović, Tamaš
AU  - Dimitrijević, Sanda
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1384
AB  - This paper describes the first documented case of cattle grub (hypodermosis) in Northern Serbia (Vojvodina). Subcutaneous warbles were determined in a six year old Simmental cow, at nine places along the spine. After the extirpation of larvae, based on the morphological characterisation, larvae of the third stage of Hypoderma bovis were diagnosed. The cow was administered therapeutic treatment, which had a favorable outcome, with no signs of recurrence. To the authors best knowledge, the case described in this paper is the first documented case of hypodermosis in cattle in Northern Serbia (Vojvodina). As the climate changed in the past few decades, it is important to pursue detailed investigations of the prevalence of this parasitic myiasis, as there are few such literature data for the Southern region of Serbia. One should also not ignore the fact that species of the genus Hypoderma can cause myiasis in humans as well.
PB  - Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje
T2  - Macedonian Veterinary Review
T1  - Hypodermosis in northern Serbia (Vojvodina)
VL  - 39
IS  - 1
SP  - 129
EP  - 133
DO  - 10.1515/macvetrev-2016-0072
ER  - 
@article{
author = "Becskei, Zsolt and Ilić, Tamara and Pavlicević, Natasa and Kiskároly, Ferenc and Petrović, Tamaš and Dimitrijević, Sanda",
year = "2016",
abstract = "This paper describes the first documented case of cattle grub (hypodermosis) in Northern Serbia (Vojvodina). Subcutaneous warbles were determined in a six year old Simmental cow, at nine places along the spine. After the extirpation of larvae, based on the morphological characterisation, larvae of the third stage of Hypoderma bovis were diagnosed. The cow was administered therapeutic treatment, which had a favorable outcome, with no signs of recurrence. To the authors best knowledge, the case described in this paper is the first documented case of hypodermosis in cattle in Northern Serbia (Vojvodina). As the climate changed in the past few decades, it is important to pursue detailed investigations of the prevalence of this parasitic myiasis, as there are few such literature data for the Southern region of Serbia. One should also not ignore the fact that species of the genus Hypoderma can cause myiasis in humans as well.",
publisher = "Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje",
journal = "Macedonian Veterinary Review",
title = "Hypodermosis in northern Serbia (Vojvodina)",
volume = "39",
number = "1",
pages = "129-133",
doi = "10.1515/macvetrev-2016-0072"
}
Becskei, Z., Ilić, T., Pavlicević, N., Kiskároly, F., Petrović, T.,& Dimitrijević, S.. (2016). Hypodermosis in northern Serbia (Vojvodina). in Macedonian Veterinary Review
Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje., 39(1), 129-133.
https://doi.org/10.1515/macvetrev-2016-0072
Becskei Z, Ilić T, Pavlicević N, Kiskároly F, Petrović T, Dimitrijević S. Hypodermosis in northern Serbia (Vojvodina). in Macedonian Veterinary Review. 2016;39(1):129-133.
doi:10.1515/macvetrev-2016-0072 .
Becskei, Zsolt, Ilić, Tamara, Pavlicević, Natasa, Kiskároly, Ferenc, Petrović, Tamaš, Dimitrijević, Sanda, "Hypodermosis in northern Serbia (Vojvodina)" in Macedonian Veterinary Review, 39, no. 1 (2016):129-133,
https://doi.org/10.1515/macvetrev-2016-0072 . .
1
1
1

Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms

Milanov, Dubravka; Petrović, Tamaš; Polaček, Vladimir; Suvajdzić, Ljiljana; Bojkovski, Jovan

(Walter De Gruyter Gmbh, Berlin, 2016)

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Polaček, Vladimir
AU  - Suvajdzić, Ljiljana
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1414
AB  - Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Journal of Veterinary Research
T1  - Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms
VL  - 60
IS  - 4
SP  - 373
EP  - 378
DO  - 10.1515/jvetres-2016-0054
ER  - 
@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Polaček, Vladimir and Suvajdzić, Ljiljana and Bojkovski, Jovan",
year = "2016",
abstract = "Increased incidence of protothecal mastitis has been recorded in several countries in the past ten years. The main goal of this article is to draw the attention of scientific and professional community to the emerging issue of mammary protothecosis. The article collates currently known facts about infection reservoirs, predisposing factors for the development of mastitis, clinical manifestations of the disease, and potential transmission routes within the herd as well as the measures for control and eradication. We would like to point out that identification of protothecal mastitis on a dairy farm is associated with a range of problems. Early detection of infected animals can be difficult because of predominantly subclinical course of early-stage infection, which easily spreads between cows via the milking system. Spontaneous recovery has not been recorded and infected cows typically develop chronic mastitis with granulomatous infiltration and progressive loss of functional parenchyma of the mammary gland. Substantial economic losses and health damages associated with mammary protothecosis strongly emphasise the need for developing effective prevention strategies aimed at control of the infection.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Journal of Veterinary Research",
title = "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms",
volume = "60",
number = "4",
pages = "373-378",
doi = "10.1515/jvetres-2016-0054"
}
Milanov, D., Petrović, T., Polaček, V., Suvajdzić, L.,& Bojkovski, J.. (2016). Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research
Walter De Gruyter Gmbh, Berlin., 60(4), 373-378.
https://doi.org/10.1515/jvetres-2016-0054
Milanov D, Petrović T, Polaček V, Suvajdzić L, Bojkovski J. Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. in Journal of Veterinary Research. 2016;60(4):373-378.
doi:10.1515/jvetres-2016-0054 .
Milanov, Dubravka, Petrović, Tamaš, Polaček, Vladimir, Suvajdzić, Ljiljana, Bojkovski, Jovan, "Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms" in Journal of Veterinary Research, 60, no. 4 (2016):373-378,
https://doi.org/10.1515/jvetres-2016-0054 . .
22
9
20

Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus

Milić, Nenad; Nišavić, Jakov; Borozan, Sunčica; Zorić, Andrea; Lazić, Sava; Petrović, Tamaš; Rašić, Zoran

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2015)

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Borozan, Sunčica
AU  - Zorić, Andrea
AU  - Lazić, Sava
AU  - Petrović, Tamaš
AU  - Rašić, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1221
AB  - The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.
AB  - Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
T1  - Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine
VL  - 69
IS  - 5-6
SP  - 337
EP  - 355
DO  - 10.2298/VETGL1506337M
ER  - 
@article{
author = "Milić, Nenad and Nišavić, Jakov and Borozan, Sunčica and Zorić, Andrea and Lazić, Sava and Petrović, Tamaš and Rašić, Zoran",
year = "2015",
abstract = "The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines., Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus, Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine",
volume = "69",
number = "5-6",
pages = "337-355",
doi = "10.2298/VETGL1506337M"
}
Milić, N., Nišavić, J., Borozan, S., Zorić, A., Lazić, S., Petrović, T.,& Rašić, Z.. (2015). Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(5-6), 337-355.
https://doi.org/10.2298/VETGL1506337M
Milić N, Nišavić J, Borozan S, Zorić A, Lazić S, Petrović T, Rašić Z. Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik. 2015;69(5-6):337-355.
doi:10.2298/VETGL1506337M .
Milić, Nenad, Nišavić, Jakov, Borozan, Sunčica, Zorić, Andrea, Lazić, Sava, Petrović, Tamaš, Rašić, Zoran, "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus" in Veterinarski Glasnik, 69, no. 5-6 (2015):337-355,
https://doi.org/10.2298/VETGL1506337M . .
1