TY  - JOUR
AU  - Ristanić, Marko
AU  - Zorc, Minja
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Blagojević, Jovan
AU  - Maletić, Milan
AU  - Stanimirović, Zoran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3706
AB  - To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it
is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic
mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research.
The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle
with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3
was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the
collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments
was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a
GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be
significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to
305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered
in proximity to genes associated with milk production traits and genomic regions under selection
pressure for other economically important traits of dairy cattle. The findings of this pilot study
provide useful information for a better understanding of the genetic architecture of milk production
traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF
cattle breeding programs.
PB  - MDPI
T2  - Animals
T1  - Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle
VL  - 14
IS  - 5
SP  - 669
DO  - 10.3390/ani14050669
ER  - 

@article{
author = "Ristanić, Marko and Zorc, Minja and Glavinić, Uroš and Stevanović, Jevrosima and Blagojević, Jovan and Maletić, Milan and Stanimirović, Zoran",
year = "2024",
abstract = "To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it
is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic
mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research.
The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle
with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3
was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the
collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments
was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a
GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be
significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to
305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered
in proximity to genes associated with milk production traits and genomic regions under selection
pressure for other economically important traits of dairy cattle. The findings of this pilot study
provide useful information for a better understanding of the genetic architecture of milk production
traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF
cattle breeding programs.",
publisher = "MDPI",
journal = "Animals",
title = "Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle",
volume = "14",
number = "5",
pages = "669",
doi = "10.3390/ani14050669"
}

Ristanić, M., Zorc, M., Glavinić, U., Stevanović, J., Blagojević, J., Maletić, M.,& Stanimirović, Z.. (2024). Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle. in Animals
MDPI., 14(5), 669.
https://doi.org/10.3390/ani14050669

Ristanić M, Zorc M, Glavinić U, Stevanović J, Blagojević J, Maletić M, Stanimirović Z. Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle. in Animals. 2024;14(5):669.
doi:10.3390/ani14050669 .

Ristanić, Marko, Zorc, Minja, Glavinić, Uroš, Stevanović, Jevrosima, Blagojević, Jovan, Maletić, Milan, Stanimirović, Zoran, "Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle" in Animals, 14, no. 5 (2024):669,
https://doi.org/10.3390/ani14050669 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja M.
AU  - Dominiković, Nina
AU  - Lakić, Nada
AU  - Ćosić, Milivoje
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3836
AB  - Nosema ceranae, a microsporidian parasite, as one of the stressors that contribute to honey bee decline, has a significant negative impact on the longevity, productivity, and reproductive capacity of honey bee colonies. There are several different strategies for Nosema infection control, including natural-based and antibiotic-based products. In this study, we tested wormwood and oak bark-based supplement “Medenko forte” on survival, Nosema infection, oxidative stress, and expression of immune-related genes in artificially N. ceranae-infected bees. The results revealed a positive influence on the survival of Nosema-infected bees, irrespectively of the moment of supplement application (day 1, day 3, or day 6 after bee emergence), as well as reduction of Nosema loads and, consequently, Nosema-induced oxidative stress. Supplementation had no negative effects on bee immunity, but better anti-Nosema than immune-stimulating effects were affirmed based on expression levels of abaecin, defensin, hymenoptaecin, apidaecin, and vitellogenin genes. In conclusion, the tested supplement „Medenko forte” has great potential in the health protection of Nosema-infected bees. However, further investigations need to be performed to elucidate its mechanisms of action.
PB  - MDPI
T2  - Animals
T1  - Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees
VL  - 14
IS  - 8
SP  - 1195
DO  - 10.3390/ani14081195
ER  - 

@article{
author = "Glavinić, Uroš and Jovanović, Nemanja M. and Dominiković, Nina and Lakić, Nada and Ćosić, Milivoje and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2024",
abstract = "Nosema ceranae, a microsporidian parasite, as one of the stressors that contribute to honey bee decline, has a significant negative impact on the longevity, productivity, and reproductive capacity of honey bee colonies. There are several different strategies for Nosema infection control, including natural-based and antibiotic-based products. In this study, we tested wormwood and oak bark-based supplement “Medenko forte” on survival, Nosema infection, oxidative stress, and expression of immune-related genes in artificially N. ceranae-infected bees. The results revealed a positive influence on the survival of Nosema-infected bees, irrespectively of the moment of supplement application (day 1, day 3, or day 6 after bee emergence), as well as reduction of Nosema loads and, consequently, Nosema-induced oxidative stress. Supplementation had no negative effects on bee immunity, but better anti-Nosema than immune-stimulating effects were affirmed based on expression levels of abaecin, defensin, hymenoptaecin, apidaecin, and vitellogenin genes. In conclusion, the tested supplement „Medenko forte” has great potential in the health protection of Nosema-infected bees. However, further investigations need to be performed to elucidate its mechanisms of action.",
publisher = "MDPI",
journal = "Animals",
title = "Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees",
volume = "14",
number = "8",
pages = "1195",
doi = "10.3390/ani14081195"
}

Glavinić, U., Jovanović, N. M., Dominiković, N., Lakić, N., Ćosić, M., Stevanović, J.,& Stanimirović, Z.. (2024). Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees. in Animals
MDPI., 14(8), 1195.
https://doi.org/10.3390/ani14081195

Glavinić U, Jovanović NM, Dominiković N, Lakić N, Ćosić M, Stevanović J, Stanimirović Z. Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees. in Animals. 2024;14(8):1195.
doi:10.3390/ani14081195 .

Glavinić, Uroš, Jovanović, Nemanja M., Dominiković, Nina, Lakić, Nada, Ćosić, Milivoje, Stevanović, Jevrosima, Stanimirović, Zoran, "Potential of wormwood and oak bark-based supplement in health improvement of Nosema ceranae-infected honey bees" in Animals, 14, no. 8 (2024):1195,
https://doi.org/10.3390/ani14081195 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Vejnović, Branislav
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2942
AB  - Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.
PB  - MDPI
T2  - Insects
T1  - Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay
VL  - 14
IS  - 5
SP  - 451
DO  - 10.3390/insects14050451
ER  - 

@article{
author = "Glavinić, Uroš and Rajković, Milan and Ristanić, Marko and Stevanović, Jevrosima and Vejnović, Branislav and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.",
publisher = "MDPI",
journal = "Insects",
title = "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay",
volume = "14",
number = "5",
pages = "451",
doi = "10.3390/insects14050451"
}

Glavinić, U., Rajković, M., Ristanić, M., Stevanović, J., Vejnović, B., Đelić, N.,& Stanimirović, Z.. (2023). Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects
MDPI., 14(5), 451.
https://doi.org/10.3390/insects14050451

Glavinić U, Rajković M, Ristanić M, Stevanović J, Vejnović B, Đelić N, Stanimirović Z. Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects. 2023;14(5):451.
doi:10.3390/insects14050451 .

Glavinić, Uroš, Rajković, Milan, Ristanić, Marko, Stevanović, Jevrosima, Vejnović, Branislav, Đelić, Ninoslav, Stanimirović, Zoran, "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay" in Insects, 14, no. 5 (2023):451,
https://doi.org/10.3390/insects14050451 . .

TY  - CONF
AU  - Jovanović, Nemanja M.
AU  - Ilić, Tamara
AU  - Glavinić, Uroš
AU  - Vejnović, Branislav
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3038
AB  - Микроспоридија Nosema ceranae је облигатни интрацелуларни паразит
медоносне пчеле (Apis mellifera) који изазива болест ноземозу при чему
негативно делује на здравље, репродуктивне и продуктивне способности пчела.
Један од најважнијих приступа у превенцији и контроли ноземозе јесте примена
биљних препарата, органских киселина, етеричних уља, полисахаридних
екстраката из печурака и пробиотика. Стога, циљ овог истраживања је био
испитивање ефеката биљних екстраката (у виду дијететског суплемента под
комерцијалним називном “B+”) на пчеле у лабораторијском (кавезном)
експерименту. Формиране су четири експерименталне групе: пчеле храњене
суплементом (Т - група), пчеле храњене суплементом и инфициране ноземом
(ИТ - група), пчеле инфициране ноземом (И група) и неинфициране пчеле (НИ).
Код свих група праћени су следећи параметри: преживљавање, број спора N.
ceranae и параметри оксидативног стреса. Сви резултати указали су на
позитиван ефекат тестираног суплемента. Наиме, добијени резултати указали
су на значајну разлику (p<0.001) у преживљавању пчела између група ИТ и И.
Поред тога, у групама инфицираних ноземом, значајно мањи (p<0.001) број
спора је утврђен у ИТ групи у односу на И групу. Налаз позитивног ефекта
исхране пчела уз додатак тестираног суплемента потврђује и значајно ниже
(p<0.001) активности антиоксидативних ензима (супероксид дисмутазе,
каталазе и глутатион С-трансферазе), као и концентрације малонилдиалдехида
у ИТ групи у односу на инфицирану (И) групу. Поред тога, у ИТ групи утврђени
су и нижи нивои (p<0.001) експресије гена за антиоксидативне ензиме у односу
на И групу. Наше истраживање је доказало позитивне ефекте испитујућег
суплемента (“B+”) на преживљавање пчела инфицираних ноземом и параметре
оксидативног стреса. Исхрана овим суплементом у Т групи није негативно
утицала на пчеле чиме је потврђено одсуство споредних нежељених ефеката.
AB  - The microsporidia Nosema ceranae is an obligate intracellular parasite of the
honeybee (Apis mellifera) that causes the disease nosemosis, which negatively affects
the health, reproduction and productive capacity of bees. One of the most important
approaches to prevent and control nosemosis is the use of herbal preparations, organic
acids, essential oils, polysaccharide extracts from mushroom, bacteria and their
metabolites. Therefore, the aim of this research was to evaluate the effects of plant
extracts (in the form of a food supplement under the trade name "B+") on bees in a
laboratory (cage) experiment. Four experimental groups were formed: Bees treated
with the supplement (T - group), bees fed with the supplement and infected with
Nosema (IT - group), bees infected with Nosema (I - group) and uninfected bees (NI).
In all groups, the following parameters were monitored: bee survival, number of N.
ceranae spores and oxidative stress parameters. All results indicated a positive effect
of the tested supplement. Indeed, the results showed a significant difference (p<0.001)
in the survival rate of the bees between groups IT and I. In addition, in the Nosema
infected groups, significantly lower (p<0.001) number of spores were recorded in the
IT group compared to the I group. The positive effect on bees fed with this supplement
is confirmed by significantly lower (p<0.001) activity of antioxidant enzymes
(superoxide dismutase, catalase and glutathione S-transferase) and
malonyldialdehyde concentrations in the IT group compared to the infected group. In
addition, lower gene expression levels (p<0.001) for antioxidant enzymes were
detected in the IT group compared to the I group. Our studies proved the positive
effects of the tested supplement on the survival of bees infected with Nosema and
oxidative stress parameters. Feeding with this supplement in the T group had no
negative effects on the bees, confirming the absence of undesirable side effects.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Efekti biljnog suplementa na oksidativni stres pčela inficiranih mikrosporidijom Nosema ceranae
T1  - Effects of plant based supplement on oxidative stress on honey bees infected with Nosema ceranae
SP  - 72
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3038
ER  - 

@conference{
author = "Jovanović, Nemanja M. and Ilić, Tamara and Glavinić, Uroš and Vejnović, Branislav and Stevanović, Jevrosima and Ristanić, Marko and Stanimirović, Zoran",
year = "2023",
abstract = "Микроспоридија Nosema ceranae је облигатни интрацелуларни паразит
медоносне пчеле (Apis mellifera) који изазива болест ноземозу при чему
негативно делује на здравље, репродуктивне и продуктивне способности пчела.
Један од најважнијих приступа у превенцији и контроли ноземозе јесте примена
биљних препарата, органских киселина, етеричних уља, полисахаридних
екстраката из печурака и пробиотика. Стога, циљ овог истраживања је био
испитивање ефеката биљних екстраката (у виду дијететског суплемента под
комерцијалним називном “B+”) на пчеле у лабораторијском (кавезном)
експерименту. Формиране су четири експерименталне групе: пчеле храњене
суплементом (Т - група), пчеле храњене суплементом и инфициране ноземом
(ИТ - група), пчеле инфициране ноземом (И група) и неинфициране пчеле (НИ).
Код свих група праћени су следећи параметри: преживљавање, број спора N.
ceranae и параметри оксидативног стреса. Сви резултати указали су на
позитиван ефекат тестираног суплемента. Наиме, добијени резултати указали
су на значајну разлику (p<0.001) у преживљавању пчела између група ИТ и И.
Поред тога, у групама инфицираних ноземом, значајно мањи (p<0.001) број
спора је утврђен у ИТ групи у односу на И групу. Налаз позитивног ефекта
исхране пчела уз додатак тестираног суплемента потврђује и значајно ниже
(p<0.001) активности антиоксидативних ензима (супероксид дисмутазе,
каталазе и глутатион С-трансферазе), као и концентрације малонилдиалдехида
у ИТ групи у односу на инфицирану (И) групу. Поред тога, у ИТ групи утврђени
су и нижи нивои (p<0.001) експресије гена за антиоксидативне ензиме у односу
на И групу. Наше истраживање је доказало позитивне ефекте испитујућег
суплемента (“B+”) на преживљавање пчела инфицираних ноземом и параметре
оксидативног стреса. Исхрана овим суплементом у Т групи није негативно
утицала на пчеле чиме је потврђено одсуство споредних нежељених ефеката., The microsporidia Nosema ceranae is an obligate intracellular parasite of the
honeybee (Apis mellifera) that causes the disease nosemosis, which negatively affects
the health, reproduction and productive capacity of bees. One of the most important
approaches to prevent and control nosemosis is the use of herbal preparations, organic
acids, essential oils, polysaccharide extracts from mushroom, bacteria and their
metabolites. Therefore, the aim of this research was to evaluate the effects of plant
extracts (in the form of a food supplement under the trade name "B+") on bees in a
laboratory (cage) experiment. Four experimental groups were formed: Bees treated
with the supplement (T - group), bees fed with the supplement and infected with
Nosema (IT - group), bees infected with Nosema (I - group) and uninfected bees (NI).
In all groups, the following parameters were monitored: bee survival, number of N.
ceranae spores and oxidative stress parameters. All results indicated a positive effect
of the tested supplement. Indeed, the results showed a significant difference (p<0.001)
in the survival rate of the bees between groups IT and I. In addition, in the Nosema
infected groups, significantly lower (p<0.001) number of spores were recorded in the
IT group compared to the I group. The positive effect on bees fed with this supplement
is confirmed by significantly lower (p<0.001) activity of antioxidant enzymes
(superoxide dismutase, catalase and glutathione S-transferase) and
malonyldialdehyde concentrations in the IT group compared to the infected group. In
addition, lower gene expression levels (p<0.001) for antioxidant enzymes were
detected in the IT group compared to the I group. Our studies proved the positive
effects of the tested supplement on the survival of bees infected with Nosema and
oxidative stress parameters. Feeding with this supplement in the T group had no
negative effects on the bees, confirming the absence of undesirable side effects.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Efekti biljnog suplementa na oksidativni stres pčela inficiranih mikrosporidijom Nosema ceranae, Effects of plant based supplement on oxidative stress on honey bees infected with Nosema ceranae",
pages = "72-73",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3038"
}

Jovanović, N. M., Ilić, T., Glavinić, U., Vejnović, B., Stevanović, J., Ristanić, M.,& Stanimirović, Z.. (2023). Efekti biljnog suplementa na oksidativni stres pčela inficiranih mikrosporidijom Nosema ceranae. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 72-73.
https://hdl.handle.net/21.15107/rcub_veterinar_3038

Jovanović NM, Ilić T, Glavinić U, Vejnović B, Stevanović J, Ristanić M, Stanimirović Z. Efekti biljnog suplementa na oksidativni stres pčela inficiranih mikrosporidijom Nosema ceranae. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:72-73.
https://hdl.handle.net/21.15107/rcub_veterinar_3038 .

Jovanović, Nemanja M., Ilić, Tamara, Glavinić, Uroš, Vejnović, Branislav, Stevanović, Jevrosima, Ristanić, Marko, Stanimirović, Zoran, "Efekti biljnog suplementa na oksidativni stres pčela inficiranih mikrosporidijom Nosema ceranae" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):72-73,
https://hdl.handle.net/21.15107/rcub_veterinar_3038 .

TY  - CONF
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Glavinić, Uroš
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3141
AB  - Uslov opstanka agroekosistema je postojanje raznolikosti živog sveta u njima, a
za očuvanje biodiverziteta neophodna je procena, verifikacija i sistematizacija
razlika na svim nivoima: genetičkom, specijskom i ekosistemskom. U ovom radu
prikazane su analize DNK i savremeni biotehnološki alati koji obezbeđuju procenu genetičkog diverziteta u agroekosistemima i posredno doprinose očuvanju
raznolikosti vrsta i ekosistema. Genomika, zbog opadanja cene sekvenciranja,
pruža sve više mogućnosti da se sa povećanom preciznošću obezbede odgovori
na genetička pitanja na koja se prethodno odgovaralo sa nekolicinom molekularnih markera. Metabarkodiranje obećava brže, jeftinije i tačnije procene
biodiverziteta. Podaci iz genomike i transkriptomike mogu da se upotrebe radi
procene potencijala populacija da se adaptiraju na nove izazove. Konačno, navedeni su problemi koji ukazuju na potrebu daljih usavršavanja postojećih
metoda genomike i transkriptomike u funkciji procene i očuvanja biodiverziteta.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Analize dnk u proceni biodiverziteta u agroekosistemima
SP  - 98
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3141
ER  - 

@conference{
author = "Stevanović, Jevrosima and Ristanić, Marko and Glavinić, Uroš and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Uslov opstanka agroekosistema je postojanje raznolikosti živog sveta u njima, a
za očuvanje biodiverziteta neophodna je procena, verifikacija i sistematizacija
razlika na svim nivoima: genetičkom, specijskom i ekosistemskom. U ovom radu
prikazane su analize DNK i savremeni biotehnološki alati koji obezbeđuju procenu genetičkog diverziteta u agroekosistemima i posredno doprinose očuvanju
raznolikosti vrsta i ekosistema. Genomika, zbog opadanja cene sekvenciranja,
pruža sve više mogućnosti da se sa povećanom preciznošću obezbede odgovori
na genetička pitanja na koja se prethodno odgovaralo sa nekolicinom molekularnih markera. Metabarkodiranje obećava brže, jeftinije i tačnije procene
biodiverziteta. Podaci iz genomike i transkriptomike mogu da se upotrebe radi
procene potencijala populacija da se adaptiraju na nove izazove. Konačno, navedeni su problemi koji ukazuju na potrebu daljih usavršavanja postojećih
metoda genomike i transkriptomike u funkciji procene i očuvanja biodiverziteta.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Analize dnk u proceni biodiverziteta u agroekosistemima",
pages = "98-108",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3141"
}

Stevanović, J., Ristanić, M., Glavinić, U., Đelić, N.,& Stanimirović, Z.. (2023). Analize dnk u proceni biodiverziteta u agroekosistemima. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 98-108.
https://hdl.handle.net/21.15107/rcub_veterinar_3141

Stevanović J, Ristanić M, Glavinić U, Đelić N, Stanimirović Z. Analize dnk u proceni biodiverziteta u agroekosistemima. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:98-108.
https://hdl.handle.net/21.15107/rcub_veterinar_3141 .

Stevanović, Jevrosima, Ristanić, Marko, Glavinić, Uroš, Đelić, Ninoslav, Stanimirović, Zoran, "Analize dnk u proceni biodiverziteta u agroekosistemima" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):98-108,
https://hdl.handle.net/21.15107/rcub_veterinar_3141 .

TY  - CONF
AU  - Stevanović, Jevrosima
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jovanović, Nemanja
AU  - Dominiković, Nina
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3221
AB  - Apiterapija je grana alternativne medicine koja za lečenje ili prevenciju zdravlja
koristi pčelinje proizvode, kako biljnog porekla (nektar, polen i biljne smole) koje su
pčele prepadile pomoću sopstvenih izlučevina i stvorile med, pergu i propolis, ali i one
koje same luče: matični mleč, vosak i apitoksin. U apiterapiji se mogu koristiti i larve
pčela (pre svega trutova), kao i vazduh iz košnica. U veterinarskoj praksi apiterapija
se može primenjivati samo nakon pregleda licenciranog veterinara.
U ovom radu fokus je stavljen na rezultate dobijene u eksperimentima na
životinjama. Tretmani medom pasa, konja, mačaka, goveda, svinja, živine, kunića,
pacova i miševa pokazali su najveću efikasnost u lečenju opekotina i rana, ali i u
lečenju gastrointestinalnih poremećaja i metaboličkog sindroma. Za propolis postoji
mnoštvo dokaza o antimikrobnom, imunomodulatornom i protivupalnom dejstvu,
zbog čega je izuzetan za saniranje rana, regeneraciju tkiva i lečenje gastričnih čireva i
crevnih upala, tretman mastitisa kod goveda, vulvo-vaginalnih kandidijaza i brojnih
stanja u usnoj duplji. Propolis ima antikancerogeni potencijal i ublažava toksične
efekte hemoterapeutika.
Pčelinji otrov se koristi u lečenju reumatoidnog artritisa, zahvaljujući
antiinflamatornom i antinociceptivnom i antiedematoznom efektu. Apitoksin ima
antikancerogeno dejstvo, a pčelinji ubodi blagotvorno deluju kod
neurodegenerativnih bolesti i neuropatija. Apitoksin poboljšava reproduktivne
performance, imunitet i zdravstveni status životinja. Oralno aplikovan stimuliše
konverziju hrane brojlera, a injekciono primenjen kod prasadi pozitivno utiče na prirast, preživljavanje i imunitet. Apitoksin ima i imunoprofilaktički, antibakterijski,
antifungalni i antivirusni efekat.
Polen se preporučuje kao dodatak ishrani, a ima potencijal u lečenju dijabetesa,
upala i hiperplazije prostate, alergija i nekih kancera. Za matičnu mleč je dokazano da
štiti životinje od toksičnih efekata hemoterapije, a ima i dokaza o njegovom
antikancerogenom potencijalu. Mleču se pripisuje dejstvo protiv starenja i u
sprečavanju osteoporoze, a dokazano je i da ublažava neurološke poremećaje.
Najpoznatiji efekat trutovskih larvi, androgeni, dokazan je kod svinja, ovaca i živine.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Apiterapija – pomoć ili alternativa veterinarskoj medicini
SP  - 279
EP  - 289
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3221
ER  - 

@conference{
author = "Stevanović, Jevrosima and Glavinić, Uroš and Ristanić, Marko and Jovanović, Nemanja and Dominiković, Nina and Stanimirović, Zoran",
year = "2023",
abstract = "Apiterapija je grana alternativne medicine koja za lečenje ili prevenciju zdravlja
koristi pčelinje proizvode, kako biljnog porekla (nektar, polen i biljne smole) koje su
pčele prepadile pomoću sopstvenih izlučevina i stvorile med, pergu i propolis, ali i one
koje same luče: matični mleč, vosak i apitoksin. U apiterapiji se mogu koristiti i larve
pčela (pre svega trutova), kao i vazduh iz košnica. U veterinarskoj praksi apiterapija
se može primenjivati samo nakon pregleda licenciranog veterinara.
U ovom radu fokus je stavljen na rezultate dobijene u eksperimentima na
životinjama. Tretmani medom pasa, konja, mačaka, goveda, svinja, živine, kunića,
pacova i miševa pokazali su najveću efikasnost u lečenju opekotina i rana, ali i u
lečenju gastrointestinalnih poremećaja i metaboličkog sindroma. Za propolis postoji
mnoštvo dokaza o antimikrobnom, imunomodulatornom i protivupalnom dejstvu,
zbog čega je izuzetan za saniranje rana, regeneraciju tkiva i lečenje gastričnih čireva i
crevnih upala, tretman mastitisa kod goveda, vulvo-vaginalnih kandidijaza i brojnih
stanja u usnoj duplji. Propolis ima antikancerogeni potencijal i ublažava toksične
efekte hemoterapeutika.
Pčelinji otrov se koristi u lečenju reumatoidnog artritisa, zahvaljujući
antiinflamatornom i antinociceptivnom i antiedematoznom efektu. Apitoksin ima
antikancerogeno dejstvo, a pčelinji ubodi blagotvorno deluju kod
neurodegenerativnih bolesti i neuropatija. Apitoksin poboljšava reproduktivne
performance, imunitet i zdravstveni status životinja. Oralno aplikovan stimuliše
konverziju hrane brojlera, a injekciono primenjen kod prasadi pozitivno utiče na prirast, preživljavanje i imunitet. Apitoksin ima i imunoprofilaktički, antibakterijski,
antifungalni i antivirusni efekat.
Polen se preporučuje kao dodatak ishrani, a ima potencijal u lečenju dijabetesa,
upala i hiperplazije prostate, alergija i nekih kancera. Za matičnu mleč je dokazano da
štiti životinje od toksičnih efekata hemoterapije, a ima i dokaza o njegovom
antikancerogenom potencijalu. Mleču se pripisuje dejstvo protiv starenja i u
sprečavanju osteoporoze, a dokazano je i da ublažava neurološke poremećaje.
Najpoznatiji efekat trutovskih larvi, androgeni, dokazan je kod svinja, ovaca i živine.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Apiterapija – pomoć ili alternativa veterinarskoj medicini",
pages = "279-289",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3221"
}

Stevanović, J., Glavinić, U., Ristanić, M., Jovanović, N., Dominiković, N.,& Stanimirović, Z.. (2023). Apiterapija – pomoć ili alternativa veterinarskoj medicini. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 279-289.
https://hdl.handle.net/21.15107/rcub_veterinar_3221

Stevanović J, Glavinić U, Ristanić M, Jovanović N, Dominiković N, Stanimirović Z. Apiterapija – pomoć ili alternativa veterinarskoj medicini. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:279-289.
https://hdl.handle.net/21.15107/rcub_veterinar_3221 .

Stevanović, Jevrosima, Glavinić, Uroš, Ristanić, Marko, Jovanović, Nemanja, Dominiković, Nina, Stanimirović, Zoran, "Apiterapija – pomoć ili alternativa veterinarskoj medicini" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):279-289,
https://hdl.handle.net/21.15107/rcub_veterinar_3221 .

TY  - CONF
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jelisić, Stefan
AU  - Blagojević, Jovan
AU  - Jovanović, Nemanja
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3222
AB  - Propolis je smeša koju proizvode pčele sakupljajući lepljive materije sa pupoljaka
biljaka i mešajuci ih sa voskom. Pčele propolisom učvršćuju saće i zatvaraju pukotine
unutar gnezda. Propolis su za lečenje i prevenciju bolesti koristile još praistorijske
civilizacije, a poznato je da su ga Stari Egipćani, Grci i Rimljani koristili za lečenje rana,
kožnih lezija i čireva. Razvoj nauke u XX i XXI veku omogućio je dokazivanje pozitivnih
efekata i mehanizama delovanja propolisa. Biološke aktivnosti propolisa u vezi su sa
hemijskim sastavom, koji varira periodično, zavisno od vegetacije. Dokazani
medicinski efekti propolisa su: antimikrobni (antibakterijski, antifungalni i
antivirusni), antioksidativni i imunoregulatorni. Dokazan je efekat propolisa kako na
Gram pozitivne bakterije (rodova Staphylococcus, Streptococcus, Kocuria), tako i na
gram negativne bakterije (rodova Haemophilus, Porphyromonas, Prevotella).
Antigljivični efekat propolisa najviše je istraživan i dokazan za rodove Candida,
Saccharomyces i Trichophyton, dok je najznačajniji antivirusni potencijal potvrđen za
Herpes Simplex Virus tip 1, influenca viruse kao i SARS-CoV-2. Kada je reč o
imunoregulatornom efektu, dokazan je inhibitorni efekat na formiranje granuloma i
eksudata, povećanje vaskularne permeabilnosti i dobar analgetski efekat kod
tretiranih životinja, sugerišući da propolis ove efekte ostvaruje inhibitornim dejstvom
na proizvodnju prostaglandina. Takođe, dokazano je da propolis dovodi do suzbijanja
leukotrijena u peritonealnim makrofagima miševa. Flavonoidi i fenolne kiselie, u
zavisnosti od porekla propolisa, čine glavne imuno-značajne komponenate propolisa.
Neki istraživači su izdvojili fenetil estar kofeinske kiseline kao veoma značajnu
imunoregulatornu komponentu propolisa. Još neki od puteva delovanja propolisa su smanjenje proizvodnje proinflamatornih citokina kao što su TNF-α, IL-6 i povećanje
IL-10, TGF-b i IL-1β. Imunoregulatorni efekti propolisa dovode ili do stimulacije, ili do
suzbijanja imunog odgovora, čineći ga potencijalno primenljivim u alternativnoj i
pomoćnoj terapiju u lečenju raznih bolesti. U ovom radu biće predstavljeni primeri
istraživanja koja su dokazala gore navedene efekte propolisa na životinjama.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Mehanizmi lekovitog dejstva propolisa u apiterapiji životinja
SP  - 290
EP  - 298
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3222
ER  - 

@conference{
author = "Glavinić, Uroš and Ristanić, Marko and Jelisić, Stefan and Blagojević, Jovan and Jovanović, Nemanja and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2023",
abstract = "Propolis je smeša koju proizvode pčele sakupljajući lepljive materije sa pupoljaka
biljaka i mešajuci ih sa voskom. Pčele propolisom učvršćuju saće i zatvaraju pukotine
unutar gnezda. Propolis su za lečenje i prevenciju bolesti koristile još praistorijske
civilizacije, a poznato je da su ga Stari Egipćani, Grci i Rimljani koristili za lečenje rana,
kožnih lezija i čireva. Razvoj nauke u XX i XXI veku omogućio je dokazivanje pozitivnih
efekata i mehanizama delovanja propolisa. Biološke aktivnosti propolisa u vezi su sa
hemijskim sastavom, koji varira periodično, zavisno od vegetacije. Dokazani
medicinski efekti propolisa su: antimikrobni (antibakterijski, antifungalni i
antivirusni), antioksidativni i imunoregulatorni. Dokazan je efekat propolisa kako na
Gram pozitivne bakterije (rodova Staphylococcus, Streptococcus, Kocuria), tako i na
gram negativne bakterije (rodova Haemophilus, Porphyromonas, Prevotella).
Antigljivični efekat propolisa najviše je istraživan i dokazan za rodove Candida,
Saccharomyces i Trichophyton, dok je najznačajniji antivirusni potencijal potvrđen za
Herpes Simplex Virus tip 1, influenca viruse kao i SARS-CoV-2. Kada je reč o
imunoregulatornom efektu, dokazan je inhibitorni efekat na formiranje granuloma i
eksudata, povećanje vaskularne permeabilnosti i dobar analgetski efekat kod
tretiranih životinja, sugerišući da propolis ove efekte ostvaruje inhibitornim dejstvom
na proizvodnju prostaglandina. Takođe, dokazano je da propolis dovodi do suzbijanja
leukotrijena u peritonealnim makrofagima miševa. Flavonoidi i fenolne kiselie, u
zavisnosti od porekla propolisa, čine glavne imuno-značajne komponenate propolisa.
Neki istraživači su izdvojili fenetil estar kofeinske kiseline kao veoma značajnu
imunoregulatornu komponentu propolisa. Još neki od puteva delovanja propolisa su smanjenje proizvodnje proinflamatornih citokina kao što su TNF-α, IL-6 i povećanje
IL-10, TGF-b i IL-1β. Imunoregulatorni efekti propolisa dovode ili do stimulacije, ili do
suzbijanja imunog odgovora, čineći ga potencijalno primenljivim u alternativnoj i
pomoćnoj terapiju u lečenju raznih bolesti. U ovom radu biće predstavljeni primeri
istraživanja koja su dokazala gore navedene efekte propolisa na životinjama.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Mehanizmi lekovitog dejstva propolisa u apiterapiji životinja",
pages = "290-298",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3222"
}

Glavinić, U., Ristanić, M., Jelisić, S., Blagojević, J., Jovanović, N., Stevanović, J.,& Stanimirović, Z.. (2023). Mehanizmi lekovitog dejstva propolisa u apiterapiji životinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 290-298.
https://hdl.handle.net/21.15107/rcub_veterinar_3222

Glavinić U, Ristanić M, Jelisić S, Blagojević J, Jovanović N, Stevanović J, Stanimirović Z. Mehanizmi lekovitog dejstva propolisa u apiterapiji životinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:290-298.
https://hdl.handle.net/21.15107/rcub_veterinar_3222 .

Glavinić, Uroš, Ristanić, Marko, Jelisić, Stefan, Blagojević, Jovan, Jovanović, Nemanja, Stevanović, Jevrosima, Stanimirović, Zoran, "Mehanizmi lekovitog dejstva propolisa u apiterapiji životinja" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):290-298,
https://hdl.handle.net/21.15107/rcub_veterinar_3222 .

TY  - CONF
AU  - Ristanić, Marko
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja
AU  - Niketić, Mia
AU  - Pejčić, Aleksa
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3223
AB  - Poznato je da se med od davnina koristio u medicinske svrhe. Poslednjih decenija ponovo se javlja interesovanje za upotrebu meda u humanoj i veterinarskoj medicini, posebno za lečenje opekotina i drugih rana, ostalih dermatoloških problema i čira na želucu izazvanog nesteroidnim-antiinflamatornim lekovima. Studije ukazuju na terapeutska svojstva meda: antioksidativna, antimikrobna, antiparazitska, antiinflamatorna, antitumorska i kardioprotektivna. Od svih, najpoznatije je antioksidativno dejstvo meda, koje potiče od polifenolnih jedinjenja (fenolne kiseline i flavonoidi), vitamina C i E, i enzima (katalaza, peroksidaza), koja su, takođe, uključena u antiinflamatorne i antikancerogene efekte meda. Antibakterijski potencijal meda je usko povezan sa niskim sadržajem vode i prisustvom glukozooksidaze, sprečavajući rast bakterija, čak i ubijajući mikroorganizme kao što su meticilin rezistentni Staphylococcus aureus (MRSA) i bakterije iz roda Pseudomonas izolovane iz inficiranih rana. Manuka med je efikasan u smanjenju upalnih procesa rana, stimulaciji angiogeneze, kao i formiranju kolagena i hiperplaziji epitela na ekstremitima kod konja. Nadalje, manuka med je smanjio ulcerozni indeks kod pacova zadržavajući enzimske (GPx i SOD) i neenzimske (GSH i NO) antioksidanse, kao i inflamatorne citokine (TNF-𝛼��, IL-1𝛽�� i IL-6) u redukovanom obliku, inhibirajući peroksidaciju lipida (MDA) i održavajući nivo glikoproteina sluzokože. Medicinski med je promovisao brz klinički napredak pasa sa dijagnostikovanim otitis externa, pri čemu je 70% pasa klinički izlečeno između 7. i 14. dana, a kod preko 90% pasa infekcija se povukla do 21. dana. Takođe, kestenov med je doprineo značajnoj reepitelizaciji i skraćivanju procesa zarastanja rana kod miševa. Imajući u vidu sve navedeno, med u različitim oblicima poseduje brojne blagotvorne efekte na zdravlje životinja i ljudi, te bi njegov potencijal trebalo iskoristiti u veterinarskoj praksi.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Primena meda u apiterapiji životinja
SP  - 299
EP  - 304
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3223
ER  - 

@conference{
author = "Ristanić, Marko and Glavinić, Uroš and Jovanović, Nemanja and Niketić, Mia and Pejčić, Aleksa and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2023",
abstract = "Poznato je da se med od davnina koristio u medicinske svrhe. Poslednjih decenija ponovo se javlja interesovanje za upotrebu meda u humanoj i veterinarskoj medicini, posebno za lečenje opekotina i drugih rana, ostalih dermatoloških problema i čira na želucu izazvanog nesteroidnim-antiinflamatornim lekovima. Studije ukazuju na terapeutska svojstva meda: antioksidativna, antimikrobna, antiparazitska, antiinflamatorna, antitumorska i kardioprotektivna. Od svih, najpoznatije je antioksidativno dejstvo meda, koje potiče od polifenolnih jedinjenja (fenolne kiseline i flavonoidi), vitamina C i E, i enzima (katalaza, peroksidaza), koja su, takođe, uključena u antiinflamatorne i antikancerogene efekte meda. Antibakterijski potencijal meda je usko povezan sa niskim sadržajem vode i prisustvom glukozooksidaze, sprečavajući rast bakterija, čak i ubijajući mikroorganizme kao što su meticilin rezistentni Staphylococcus aureus (MRSA) i bakterije iz roda Pseudomonas izolovane iz inficiranih rana. Manuka med je efikasan u smanjenju upalnih procesa rana, stimulaciji angiogeneze, kao i formiranju kolagena i hiperplaziji epitela na ekstremitima kod konja. Nadalje, manuka med je smanjio ulcerozni indeks kod pacova zadržavajući enzimske (GPx i SOD) i neenzimske (GSH i NO) antioksidanse, kao i inflamatorne citokine (TNF-𝛼��, IL-1𝛽�� i IL-6) u redukovanom obliku, inhibirajući peroksidaciju lipida (MDA) i održavajući nivo glikoproteina sluzokože. Medicinski med je promovisao brz klinički napredak pasa sa dijagnostikovanim otitis externa, pri čemu je 70% pasa klinički izlečeno između 7. i 14. dana, a kod preko 90% pasa infekcija se povukla do 21. dana. Takođe, kestenov med je doprineo značajnoj reepitelizaciji i skraćivanju procesa zarastanja rana kod miševa. Imajući u vidu sve navedeno, med u različitim oblicima poseduje brojne blagotvorne efekte na zdravlje životinja i ljudi, te bi njegov potencijal trebalo iskoristiti u veterinarskoj praksi.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Primena meda u apiterapiji životinja",
pages = "299-304",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3223"
}

Ristanić, M., Glavinić, U., Jovanović, N., Niketić, M., Pejčić, A., Stevanović, J.,& Stanimirović, Z.. (2023). Primena meda u apiterapiji životinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 299-304.
https://hdl.handle.net/21.15107/rcub_veterinar_3223

Ristanić M, Glavinić U, Jovanović N, Niketić M, Pejčić A, Stevanović J, Stanimirović Z. Primena meda u apiterapiji životinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:299-304.
https://hdl.handle.net/21.15107/rcub_veterinar_3223 .

Ristanić, Marko, Glavinić, Uroš, Jovanović, Nemanja, Niketić, Mia, Pejčić, Aleksa, Stevanović, Jevrosima, Stanimirović, Zoran, "Primena meda u apiterapiji životinja" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):299-304,
https://hdl.handle.net/21.15107/rcub_veterinar_3223 .

TY  - CONF
AU  - Jovanović, Nemanja M.
AU  - Aleksić, Nevenka
AU  - Ilić, Tamara
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3224
AB  - Prirodna jedinjenja, koja imaju brojna farmakodinamska dejstva, mogu biti ključ
za rešenje problema rezistencije parazita na antiparazitske lekove, koja je sve češće
prisutna i opisivana u stručnoj i naučnoj literaturi. Poslednjih decenija postoji veliko
interesovanje za ispitivanje farmakodinamskih i hemijskih karakteristika pčelinjih
proizvoda koji su izvor prirodnih bioaktivnih supstanci. Dokazano je da ovi proizvodi
mogu ispoljiti raznolike pozititivne efekte u organizmu ljudi i životinja. Pčelinji
proizvodi ‐ propolis, apitoksin, polen i med ‐ mogu imati i izvesno antiparazitsko
dejstvo protiv određenih vrsta protozoa i helminata. Lekovita svojstva navedenih
proizvoda uslovljena su prisustvom i koncentracijom određenih hemijskih supstanci,
koji se razlikuju u zavisnosti od geografskog porekla i florističkog sastava u određenom
regionu, na šta ukazuju regionalne varijacije u njihovom antiparazitskom delovanju.
Pretpostavlja se da postoji nekoliko mogućih mehanizama kojim pčelinji proizvodi
deluju protiv infekcija prouzrokovanih parazitima. To su: aktiviranje makrofaga koji
ubijaju parazite stvaranjem reaktivnih kiseoničnih i azotnih vrsta (propolis, polen);
inhibicija angiogeneze u inficiranom tkivu (propolis); različiti imunomodulatorni
efekti; indukovanje mehanizama apoptoze u ćelijama parazita (propolis, med);
sprečavanje prodiranja parazita u ćeliju domaćina formiranjem fizičke barijere ili
inhibiranjem proteina i enzima neophodnih za invaziju parazita (propolis);
narušavanje integriteta membrana i organela ćelija parazita (propolis). Ključnu ulogu
u ostvarivanju ovih efekata igraju flavonoidi i fenolna jedinjenja sadržana u propolisu, polenu, medu i apitoksinu. Istraživanja izvedena većinom in vitro i na
eksperimentalnim životinjama dokazala su antiparazitsku efikasnost pojedinih
pčelinjih proizvoda. Ukoliko se ovaj potencijal potvrdi daljim ispitivanjima, može se
očekivati da će se bioaktivne komponente izolovane iz pčelinjih proizvoda moći
koristiti za alternativni pristup rešavanju problema parazitskih infekcija.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Antiparazitski potencijal pčelinjih proizvoda
SP  - 310
EP  - 316
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3224
ER  - 

@conference{
author = "Jovanović, Nemanja M. and Aleksić, Nevenka and Ilić, Tamara and Glavinić, Uroš and Ristanić, Marko and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2023",
abstract = "Prirodna jedinjenja, koja imaju brojna farmakodinamska dejstva, mogu biti ključ
za rešenje problema rezistencije parazita na antiparazitske lekove, koja je sve češće
prisutna i opisivana u stručnoj i naučnoj literaturi. Poslednjih decenija postoji veliko
interesovanje za ispitivanje farmakodinamskih i hemijskih karakteristika pčelinjih
proizvoda koji su izvor prirodnih bioaktivnih supstanci. Dokazano je da ovi proizvodi
mogu ispoljiti raznolike pozititivne efekte u organizmu ljudi i životinja. Pčelinji
proizvodi ‐ propolis, apitoksin, polen i med ‐ mogu imati i izvesno antiparazitsko
dejstvo protiv određenih vrsta protozoa i helminata. Lekovita svojstva navedenih
proizvoda uslovljena su prisustvom i koncentracijom određenih hemijskih supstanci,
koji se razlikuju u zavisnosti od geografskog porekla i florističkog sastava u određenom
regionu, na šta ukazuju regionalne varijacije u njihovom antiparazitskom delovanju.
Pretpostavlja se da postoji nekoliko mogućih mehanizama kojim pčelinji proizvodi
deluju protiv infekcija prouzrokovanih parazitima. To su: aktiviranje makrofaga koji
ubijaju parazite stvaranjem reaktivnih kiseoničnih i azotnih vrsta (propolis, polen);
inhibicija angiogeneze u inficiranom tkivu (propolis); različiti imunomodulatorni
efekti; indukovanje mehanizama apoptoze u ćelijama parazita (propolis, med);
sprečavanje prodiranja parazita u ćeliju domaćina formiranjem fizičke barijere ili
inhibiranjem proteina i enzima neophodnih za invaziju parazita (propolis);
narušavanje integriteta membrana i organela ćelija parazita (propolis). Ključnu ulogu
u ostvarivanju ovih efekata igraju flavonoidi i fenolna jedinjenja sadržana u propolisu, polenu, medu i apitoksinu. Istraživanja izvedena većinom in vitro i na
eksperimentalnim životinjama dokazala su antiparazitsku efikasnost pojedinih
pčelinjih proizvoda. Ukoliko se ovaj potencijal potvrdi daljim ispitivanjima, može se
očekivati da će se bioaktivne komponente izolovane iz pčelinjih proizvoda moći
koristiti za alternativni pristup rešavanju problema parazitskih infekcija.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Antiparazitski potencijal pčelinjih proizvoda",
pages = "310-316",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3224"
}

Jovanović, N. M., Aleksić, N., Ilić, T., Glavinić, U., Ristanić, M., Stevanović, J.,& Stanimirović, Z.. (2023). Antiparazitski potencijal pčelinjih proizvoda. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 310-316.
https://hdl.handle.net/21.15107/rcub_veterinar_3224

Jovanović NM, Aleksić N, Ilić T, Glavinić U, Ristanić M, Stevanović J, Stanimirović Z. Antiparazitski potencijal pčelinjih proizvoda. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:310-316.
https://hdl.handle.net/21.15107/rcub_veterinar_3224 .

Jovanović, Nemanja M., Aleksić, Nevenka, Ilić, Tamara, Glavinić, Uroš, Ristanić, Marko, Stevanović, Jevrosima, Stanimirović, Zoran, "Antiparazitski potencijal pčelinjih proizvoda" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):310-316,
https://hdl.handle.net/21.15107/rcub_veterinar_3224 .

TY  - JOUR
AU  - Jovanović, Nemanja M.
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Vejnović, Branislav
AU  - Ilić, Tamara
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3370
AB  - One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).
PB  - MDPI
T2  - Animals
T1  - Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae
VL  - 13
IS  - 22
SP  - 3543
DO  - 10.3390/ani13223543
ER  - 

@article{
author = "Jovanović, Nemanja M. and Glavinić, Uroš and Ristanić, Marko and Vejnović, Branislav and Ilić, Tamara and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2023",
abstract = "One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).",
publisher = "MDPI",
journal = "Animals",
title = "Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae",
volume = "13",
number = "22",
pages = "3543",
doi = "10.3390/ani13223543"
}

Jovanović, N. M., Glavinić, U., Ristanić, M., Vejnović, B., Ilić, T., Stevanović, J.,& Stanimirović, Z.. (2023). Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae. in Animals
MDPI., 13(22), 3543.
https://doi.org/10.3390/ani13223543

Jovanović NM, Glavinić U, Ristanić M, Vejnović B, Ilić T, Stevanović J, Stanimirović Z. Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae. in Animals. 2023;13(22):3543.
doi:10.3390/ani13223543 .

Jovanović, Nemanja M., Glavinić, Uroš, Ristanić, Marko, Vejnović, Branislav, Ilić, Tamara, Stevanović, Jevrosima, Stanimirović, Zoran, "Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae" in Animals, 13, no. 22 (2023):3543,
https://doi.org/10.3390/ani13223543 . .

TY  - JOUR
AU  - Brodschneider, Robert
AU  - Schlagbauer, Johannes
AU  - Arakelyan, Iliyana
AU  - Ballis, Alexis
AU  - Brus, Jan
AU  - Brusbardis, Valters
AU  - Cadahía, Luis
AU  - Charrière, Jean-Daniel
AU  - Chlebo, Robert
AU  - Coffey, Mary F.
AU  - Cornelissen, Bram
AU  - da Costa, Cristina Amaro
AU  - Danneels, Ellen
AU  - Danihlík, Jiří
AU  - Dobrescu, Constantin
AU  - Evans, Garth
AU  - Fedoriak, Mariia
AU  - Forsythe, Ivan
AU  - Gregorc, Aleš
AU  - Johannesen, Jes
AU  - Kauko, Lassi
AU  - Kristiansen, Preben
AU  - Martikkala, Maritta
AU  - Martín-Hernández, Raquel
AU  - Mazur, Ewa
AU  - Mutinelli, Franco
AU  - Patalano, Solenn
AU  - Raudmets, Aivar
AU  - Simon Delso, Noa
AU  - Stevanović, Jevrosima
AU  - Uzunov, Aleksandar
AU  - Vejsnæs, Flemming
AU  - Williams, Anthony
AU  - Gray, Alison
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2469
AB  - Beekeepers have various options to control the parasitic mite Varroa destructor in honey bee colonies, but no empirical data are available on the methods they apply in practice. We surveyed 28,409 beekeepers maintaining 507,641 colonies in 30 European countries concerning Varroa control methods. The set of 19 different Varroa diagnosis and control measures was taken from the annual COLOSS questionnaire on honey bee colony losses. The most frequent activities were monitoring of Varroa infestations, drone brood removal, various oxalic acid applications and formic acid applications. Correspondence analysis and hierarchical clustering on principal components showed that six Varroa control options (not necessarily the most used ones) significantly contribute to defining three distinctive clusters of countries in terms of Varroa control in Europe. Cluster I (eight Western European countries) is characterized by use of amitraz strips. Cluster II comprises 15 countries from Scandinavia, the Baltics, and Central-Southern Europe. This cluster is characterized by long-term formic acid treatments. Cluster III is characterized by dominant usage of amitraz fumigation and formed by seven Eastern European countries. The median number of different treatments applied per beekeeper was lowest in cluster III. Based on estimation of colony numbers in included countries, we extrapolated the proportions of colonies treated with different methods in Europe. This suggests that circa 62% of colonies in Europe are treated with amitraz, followed by oxalic acid for the next largest percentage of colonies. We discuss possible factors determining the choice of Varroa control measures in the different clusters.
PB  - Springer
T2  - Journal of Pest Science
T1  - Spatial clusters of Varroa destructor control strategies in Europe
VL  - 96
SP  - 759
EP  - 783
DO  - 10.1007/s10340-022-01523-2
ER  - 

@article{
author = "Brodschneider, Robert and Schlagbauer, Johannes and Arakelyan, Iliyana and Ballis, Alexis and Brus, Jan and Brusbardis, Valters and Cadahía, Luis and Charrière, Jean-Daniel and Chlebo, Robert and Coffey, Mary F. and Cornelissen, Bram and da Costa, Cristina Amaro and Danneels, Ellen and Danihlík, Jiří and Dobrescu, Constantin and Evans, Garth and Fedoriak, Mariia and Forsythe, Ivan and Gregorc, Aleš and Johannesen, Jes and Kauko, Lassi and Kristiansen, Preben and Martikkala, Maritta and Martín-Hernández, Raquel and Mazur, Ewa and Mutinelli, Franco and Patalano, Solenn and Raudmets, Aivar and Simon Delso, Noa and Stevanović, Jevrosima and Uzunov, Aleksandar and Vejsnæs, Flemming and Williams, Anthony and Gray, Alison",
year = "2023",
abstract = "Beekeepers have various options to control the parasitic mite Varroa destructor in honey bee colonies, but no empirical data are available on the methods they apply in practice. We surveyed 28,409 beekeepers maintaining 507,641 colonies in 30 European countries concerning Varroa control methods. The set of 19 different Varroa diagnosis and control measures was taken from the annual COLOSS questionnaire on honey bee colony losses. The most frequent activities were monitoring of Varroa infestations, drone brood removal, various oxalic acid applications and formic acid applications. Correspondence analysis and hierarchical clustering on principal components showed that six Varroa control options (not necessarily the most used ones) significantly contribute to defining three distinctive clusters of countries in terms of Varroa control in Europe. Cluster I (eight Western European countries) is characterized by use of amitraz strips. Cluster II comprises 15 countries from Scandinavia, the Baltics, and Central-Southern Europe. This cluster is characterized by long-term formic acid treatments. Cluster III is characterized by dominant usage of amitraz fumigation and formed by seven Eastern European countries. The median number of different treatments applied per beekeeper was lowest in cluster III. Based on estimation of colony numbers in included countries, we extrapolated the proportions of colonies treated with different methods in Europe. This suggests that circa 62% of colonies in Europe are treated with amitraz, followed by oxalic acid for the next largest percentage of colonies. We discuss possible factors determining the choice of Varroa control measures in the different clusters.",
publisher = "Springer",
journal = "Journal of Pest Science",
title = "Spatial clusters of Varroa destructor control strategies in Europe",
volume = "96",
pages = "759-783",
doi = "10.1007/s10340-022-01523-2"
}

Brodschneider, R., Schlagbauer, J., Arakelyan, I., Ballis, A., Brus, J., Brusbardis, V., Cadahía, L., Charrière, J., Chlebo, R., Coffey, M. F., Cornelissen, B., da Costa, C. A., Danneels, E., Danihlík, J., Dobrescu, C., Evans, G., Fedoriak, M., Forsythe, I., Gregorc, A., Johannesen, J., Kauko, L., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mazur, E., Mutinelli, F., Patalano, S., Raudmets, A., Simon Delso, N., Stevanović, J., Uzunov, A., Vejsnæs, F., Williams, A.,& Gray, A.. (2023). Spatial clusters of Varroa destructor control strategies in Europe. in Journal of Pest Science
Springer., 96, 759-783.
https://doi.org/10.1007/s10340-022-01523-2

Brodschneider R, Schlagbauer J, Arakelyan I, Ballis A, Brus J, Brusbardis V, Cadahía L, Charrière J, Chlebo R, Coffey MF, Cornelissen B, da Costa CA, Danneels E, Danihlík J, Dobrescu C, Evans G, Fedoriak M, Forsythe I, Gregorc A, Johannesen J, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Mazur E, Mutinelli F, Patalano S, Raudmets A, Simon Delso N, Stevanović J, Uzunov A, Vejsnæs F, Williams A, Gray A. Spatial clusters of Varroa destructor control strategies in Europe. in Journal of Pest Science. 2023;96:759-783.
doi:10.1007/s10340-022-01523-2 .

Brodschneider, Robert, Schlagbauer, Johannes, Arakelyan, Iliyana, Ballis, Alexis, Brus, Jan, Brusbardis, Valters, Cadahía, Luis, Charrière, Jean-Daniel, Chlebo, Robert, Coffey, Mary F., Cornelissen, Bram, da Costa, Cristina Amaro, Danneels, Ellen, Danihlík, Jiří, Dobrescu, Constantin, Evans, Garth, Fedoriak, Mariia, Forsythe, Ivan, Gregorc, Aleš, Johannesen, Jes, Kauko, Lassi, Kristiansen, Preben, Martikkala, Maritta, Martín-Hernández, Raquel, Mazur, Ewa, Mutinelli, Franco, Patalano, Solenn, Raudmets, Aivar, Simon Delso, Noa, Stevanović, Jevrosima, Uzunov, Aleksandar, Vejsnæs, Flemming, Williams, Anthony, Gray, Alison, "Spatial clusters of Varroa destructor control strategies in Europe" in Journal of Pest Science, 96 (2023):759-783,
https://doi.org/10.1007/s10340-022-01523-2 . .

TY  - JOUR
AU  - Gray, Alison
AU  - Adjlane, Noureddine
AU  - Arab, Alireza
AU  - Ballis, Alexis
AU  - Brusbardis, Valters
AU  - Bugeja Douglas, Adrian
AU  - Cadahía, Luis
AU  - Charrière, Jean-Daniel
AU  - Chlebo, Robert
AU  - Coffey, Mary F.
AU  - Cornelissen, Bram
AU  - Costa, Cristina Amaro da
AU  - Danneels, Ellen
AU  - Danihlík, Jiří
AU  - Dobrescu, Constantin
AU  - Evans, Garth
AU  - Fedoriak, Mariia
AU  - Forsythe, Ivan
AU  - Gregorc, Aleš
AU  - Ilieva Arakelyan, Iliyana
AU  - Johannesen, Jes
AU  - Kauko, Lassi
AU  - Kristiansen, Preben
AU  - Martikkala, Maritta
AU  - Martín-Hernández, Raquel
AU  - Mazur, Ewa
AU  - Medina-Flores, Carlos Aurelio
AU  - Mutinelli, Franco
AU  - Omar, Eslam M.
AU  - Patalano, Solenn
AU  - Raudmets, Aivar
AU  - San Martin, Gilles
AU  - Soroker, Victoria
AU  - Stahlmann-Brown, Philip
AU  - Stevanović, Jevrosima
AU  - Uzunov, Aleksandar
AU  - Vejsnaes, Flemming
AU  - Williams, Anthony
AU  - Brodschneider, Robert
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2504
AB  - This article presents managed honey bee colony loss rates over winter 2019/20 resulting from using the standardised COLOSS questionnaire in 37 countries. Six countries were from outside Europe, including, for the first time in this series of articles, New Zealand. The 30,491 beekeepers outside New Zealand reported 4.5% of colonies with unsolvable queen problems, 11.1% of colonies dead after winter and 2.6% lost through natural disaster. This gave an overall colony winter loss rate of 18.1%, higher than in the previous year. The winter loss rates varied greatly between countries, from 7.4% to 36.5%. 3216 beekeepers from New Zealand managing 297,345 colonies reported 10.5% losses for their 2019 winter (six months earlier than for other, Northern Hemisphere, countries). We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, for all countries except New Zealand. Overall, larger beekeeping operations with more than 50 colonies experienced significantly lower losses (p < 0.001). Migration was also highly significant (p < 0.001), with lower loss rates for operations migrating their colonies in the previous season. A higher proportion of new queens reduced the risk of colony winter loss (p < 0.001), suggesting that more queen replacement is better. All three factors, operation size, migration and proportion of young queens, were also included in a multivariable main effects quasi-binomial GLM and all three remained highly significant (p < 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at the regional level.
T2  - Journal of Apicultural Research
T1  - Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement
VL  - 62
IS  - 2
SP  - 204
EP  - 210
DO  - 10.1080/00218839.2022.2113329
ER  - 

@article{
author = "Gray, Alison and Adjlane, Noureddine and Arab, Alireza and Ballis, Alexis and Brusbardis, Valters and Bugeja Douglas, Adrian and Cadahía, Luis and Charrière, Jean-Daniel and Chlebo, Robert and Coffey, Mary F. and Cornelissen, Bram and Costa, Cristina Amaro da and Danneels, Ellen and Danihlík, Jiří and Dobrescu, Constantin and Evans, Garth and Fedoriak, Mariia and Forsythe, Ivan and Gregorc, Aleš and Ilieva Arakelyan, Iliyana and Johannesen, Jes and Kauko, Lassi and Kristiansen, Preben and Martikkala, Maritta and Martín-Hernández, Raquel and Mazur, Ewa and Medina-Flores, Carlos Aurelio and Mutinelli, Franco and Omar, Eslam M. and Patalano, Solenn and Raudmets, Aivar and San Martin, Gilles and Soroker, Victoria and Stahlmann-Brown, Philip and Stevanović, Jevrosima and Uzunov, Aleksandar and Vejsnaes, Flemming and Williams, Anthony and Brodschneider, Robert",
year = "2023",
abstract = "This article presents managed honey bee colony loss rates over winter 2019/20 resulting from using the standardised COLOSS questionnaire in 37 countries. Six countries were from outside Europe, including, for the first time in this series of articles, New Zealand. The 30,491 beekeepers outside New Zealand reported 4.5% of colonies with unsolvable queen problems, 11.1% of colonies dead after winter and 2.6% lost through natural disaster. This gave an overall colony winter loss rate of 18.1%, higher than in the previous year. The winter loss rates varied greatly between countries, from 7.4% to 36.5%. 3216 beekeepers from New Zealand managing 297,345 colonies reported 10.5% losses for their 2019 winter (six months earlier than for other, Northern Hemisphere, countries). We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, for all countries except New Zealand. Overall, larger beekeeping operations with more than 50 colonies experienced significantly lower losses (p < 0.001). Migration was also highly significant (p < 0.001), with lower loss rates for operations migrating their colonies in the previous season. A higher proportion of new queens reduced the risk of colony winter loss (p < 0.001), suggesting that more queen replacement is better. All three factors, operation size, migration and proportion of young queens, were also included in a multivariable main effects quasi-binomial GLM and all three remained highly significant (p < 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at the regional level.",
journal = "Journal of Apicultural Research",
title = "Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement",
volume = "62",
number = "2",
pages = "204-210",
doi = "10.1080/00218839.2022.2113329"
}

Gray, A., Adjlane, N., Arab, A., Ballis, A., Brusbardis, V., Bugeja Douglas, A., Cadahía, L., Charrière, J., Chlebo, R., Coffey, M. F., Cornelissen, B., Costa, C. A. d., Danneels, E., Danihlík, J., Dobrescu, C., Evans, G., Fedoriak, M., Forsythe, I., Gregorc, A., Ilieva Arakelyan, I., Johannesen, J., Kauko, L., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mazur, E., Medina-Flores, C. A., Mutinelli, F., Omar, E. M., Patalano, S., Raudmets, A., San Martin, G., Soroker, V., Stahlmann-Brown, P., Stevanović, J., Uzunov, A., Vejsnaes, F., Williams, A.,& Brodschneider, R.. (2023). Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement. in Journal of Apicultural Research, 62(2), 204-210.
https://doi.org/10.1080/00218839.2022.2113329

Gray A, Adjlane N, Arab A, Ballis A, Brusbardis V, Bugeja Douglas A, Cadahía L, Charrière J, Chlebo R, Coffey MF, Cornelissen B, Costa CAD, Danneels E, Danihlík J, Dobrescu C, Evans G, Fedoriak M, Forsythe I, Gregorc A, Ilieva Arakelyan I, Johannesen J, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Mazur E, Medina-Flores CA, Mutinelli F, Omar EM, Patalano S, Raudmets A, San Martin G, Soroker V, Stahlmann-Brown P, Stevanović J, Uzunov A, Vejsnaes F, Williams A, Brodschneider R. Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement. in Journal of Apicultural Research. 2023;62(2):204-210.
doi:10.1080/00218839.2022.2113329 .

Gray, Alison, Adjlane, Noureddine, Arab, Alireza, Ballis, Alexis, Brusbardis, Valters, Bugeja Douglas, Adrian, Cadahía, Luis, Charrière, Jean-Daniel, Chlebo, Robert, Coffey, Mary F., Cornelissen, Bram, Costa, Cristina Amaro da, Danneels, Ellen, Danihlík, Jiří, Dobrescu, Constantin, Evans, Garth, Fedoriak, Mariia, Forsythe, Ivan, Gregorc, Aleš, Ilieva Arakelyan, Iliyana, Johannesen, Jes, Kauko, Lassi, Kristiansen, Preben, Martikkala, Maritta, Martín-Hernández, Raquel, Mazur, Ewa, Medina-Flores, Carlos Aurelio, Mutinelli, Franco, Omar, Eslam M., Patalano, Solenn, Raudmets, Aivar, San Martin, Gilles, Soroker, Victoria, Stahlmann-Brown, Philip, Stevanović, Jevrosima, Uzunov, Aleksandar, Vejsnaes, Flemming, Williams, Anthony, Brodschneider, Robert, "Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement" in Journal of Apicultural Research, 62, no. 2 (2023):204-210,
https://doi.org/10.1080/00218839.2022.2113329 . .

TY  - CONF
AU  - Vejnović, Branislav
AU  - Stevanović, Jevrosima
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Mirilović, Milorad
AU  - Đurić, Spomenka
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2787
AB  - Pčelari imaju na raspolaganju različite metode za suzbijanje pčelinjeg krpelja
Varroa destructor. Kako bi se utvrdilo koje metode se koriste u pčelinjacima u Republici
Srbiji, anketirano je u okviru upitnika COLOSS za 2021. godinu, 112 pčelara koji ukupno
imaju 12 912 pčelinjih društava. Od 18 različitih kontrolnih mera protiv V. destructor
najčešće korišćene metode su bile: primena oksalne kiseline (nakapavanje i sublimacija),
primena amitraza (nadimljavanje/aerosol i preko traka), primena kumafosa (preko
traka), uklanjanje trutovskog legla i primena mravlje kiseline (kratkoročni i dugoročni
tretman). U odnosu na broj košnica, najčešće je korišćen amitraz (nadimljavanje/
aerosol i preko traka), oksalna kiselina (nakapavanje i sublimacija), kumafos (preko
traka) i mravlja kiselina (kratkoročni i dugoročni tretman), Tau-fluvalinat i uklanjanje
trutovskog legla. Na osnovu dobijenih rezultata, zaključeno je da pčelari u Srbiji protiv
Varroa destructor najčešće koriste amitraz (nadimljavanjem/aerosol i preko traka).
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje veterinara Srbije, Zlatibor, 08 - 11. septembar 2022
T1  - Strategija kontrole Varroa Destructor u Republici Srbiji
SP  - 498
EP  - 506
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2787
ER  - 

@conference{
author = "Vejnović, Branislav and Stevanović, Jevrosima and Glavinić, Uroš and Ristanić, Marko and Mirilović, Milorad and Đurić, Spomenka and Stanimirović, Zoran",
year = "2022",
abstract = "Pčelari imaju na raspolaganju različite metode za suzbijanje pčelinjeg krpelja
Varroa destructor. Kako bi se utvrdilo koje metode se koriste u pčelinjacima u Republici
Srbiji, anketirano je u okviru upitnika COLOSS za 2021. godinu, 112 pčelara koji ukupno
imaju 12 912 pčelinjih društava. Od 18 različitih kontrolnih mera protiv V. destructor
najčešće korišćene metode su bile: primena oksalne kiseline (nakapavanje i sublimacija),
primena amitraza (nadimljavanje/aerosol i preko traka), primena kumafosa (preko
traka), uklanjanje trutovskog legla i primena mravlje kiseline (kratkoročni i dugoročni
tretman). U odnosu na broj košnica, najčešće je korišćen amitraz (nadimljavanje/
aerosol i preko traka), oksalna kiselina (nakapavanje i sublimacija), kumafos (preko
traka) i mravlja kiselina (kratkoročni i dugoročni tretman), Tau-fluvalinat i uklanjanje
trutovskog legla. Na osnovu dobijenih rezultata, zaključeno je da pčelari u Srbiji protiv
Varroa destructor najčešće koriste amitraz (nadimljavanjem/aerosol i preko traka).",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje veterinara Srbije, Zlatibor, 08 - 11. septembar 2022",
title = "Strategija kontrole Varroa Destructor u Republici Srbiji",
pages = "498-506",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2787"
}

Vejnović, B., Stevanović, J., Glavinić, U., Ristanić, M., Mirilović, M., Đurić, S.,& Stanimirović, Z.. (2022). Strategija kontrole Varroa Destructor u Republici Srbiji. in 33. Savetovanje veterinara Srbije, Zlatibor, 08 - 11. septembar 2022
Beograd : Srpsko veterinarsko društvo., 498-506.
https://hdl.handle.net/21.15107/rcub_veterinar_2787

Vejnović B, Stevanović J, Glavinić U, Ristanić M, Mirilović M, Đurić S, Stanimirović Z. Strategija kontrole Varroa Destructor u Republici Srbiji. in 33. Savetovanje veterinara Srbije, Zlatibor, 08 - 11. septembar 2022. 2022;:498-506.
https://hdl.handle.net/21.15107/rcub_veterinar_2787 .

Vejnović, Branislav, Stevanović, Jevrosima, Glavinić, Uroš, Ristanić, Marko, Mirilović, Milorad, Đurić, Spomenka, Stanimirović, Zoran, "Strategija kontrole Varroa Destructor u Republici Srbiji" in 33. Savetovanje veterinara Srbije, Zlatibor, 08 - 11. septembar 2022 (2022):498-506,
https://hdl.handle.net/21.15107/rcub_veterinar_2787 .

TY  - GEN
AU  - Glavinić, Uroš
AU  - Vunduk, Jovana
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Stevanović, Jevrosima
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3039
T2  - Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije
T1  - Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3039
ER  - 

@misc{
author = "Glavinić, Uroš and Vunduk, Jovana and Ristanić, Marko and Rajković, Milan and Stevanović, Jevrosima and Vejnović, Branislav and Stanimirović, Zoran",
year = "2022",
journal = "Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije",
title = "Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3039"
}

Glavinić, U., Vunduk, J., Ristanić, M., Rajković, M., Stevanović, J., Vejnović, B.,& Stanimirović, Z.. (2022). Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus. in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.
https://hdl.handle.net/21.15107/rcub_veterinar_3039

Glavinić U, Vunduk J, Ristanić M, Rajković M, Stevanović J, Vejnović B, Stanimirović Z. Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus. in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_3039 .

Glavinić, Uroš, Vunduk, Jovana, Ristanić, Marko, Rajković, Milan, Stevanović, Jevrosima, Vejnović, Branislav, Stanimirović, Zoran, "Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus" in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3039 .

TY  - CONF
AU  - Jovanović, Nemanja M.
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Ilić, Tamara
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3031
AB  - Један од водећих узрока губитака пчелињих заједница је неадекватна исхрана у
погледу квалитета, квантитета и разноврсности, што води повећању осетљивости
на патогене и пестициде. Одузимањем превелике количине меда и прихрана пчела
са чистим шећерним сирупом изазива енергетски и оксидативни стрес што
последично води ка повећаној осетљивости пчела на патогене, првенствено на
ендопаразита Nosema ceranae. У одсуству адекватне хране у пчелињим
заједницама, често се користе суплементи, чиме се може повећати отпорност пчела
према патогенима и одржавати добро кондиционо стање пчелињих заједница. Циљ
овог истраживања је био испитивање ефеката биљног суплемента (комерцијални
назив B+) у кошничким условима, анализирајући параметре јачине пчелињих
заједница (површина отвореног и затвореног легла, резерве меда и полена и број
адултних пчела) и количину ендопаразита N. ceranae. Експеримент је изведен у две
фазе, прва фаза у августу 2018. године и друга фаза у марту 2019. године.
Поређењем праћених параметара на крају прве фазе, уочено је повећање (p < 0,05)
резерви меда и полена и броја адултних пчела у заједницама прихрањиваним
суплементом B+. Током друге фазе, вредности свих посматраних параметара су
биле значајно више (p < 0,05) у заједницама које су у исхрани користиле суплемент
B+ у односу на оне које суплемент нису добијале. Додатно, број спора N. ceranae је
током експеримента био значајно нижи (p < 0,05) у заједницама прихрањиваним
суплементом B+ у односу на оне храњене без додатка суплемента. На основу
добијених резултата утврђени су позитивни ефекти код заједница које су кроз
исхрану користиле тестирани суплемент јер се на тај начин обезбеђују хранљиви
елементи којих нема у чистом шећерном сирупу. Коришћењем овог суплемента у
јесењој прихрани пчела превенира се развој микроспоридије N. ceranae, чиме се
пчелиње заједнице знатно боље зазимљавају. У пролећној прихрани, постиже се
боља припремљеност заједница за главну пашу и побољшање производних и
репродуктивних параметара пчелињих заједница
AB  - One of the main causes of bee colony losses is inadequate nutrition in terms of quality,
quantity and diversity, which leads to increased susceptibility to pathogens and
pesticides. Taking too much honey and feeding bees with pure saccharose syrup
causes energetic and oxidative stress, which in turn leads to increased sensitivity of
bees to pathogens, primarily the endoparasite Nosema ceranae. In the absence of
adequate food in bee colonies, supplements are often used, which can increase the
resistance of bees to pathogens and maintain good health condition of bee colonies.
The aim of this study was to examine the effects of herbal supplement (commercial
name B+) in hive conditions, analysing the colonies strength parameters (open and
sealed brood area; honey and pollen reserves and number of adult bees) and level of
N. ceranae. The experiment was conducted in two phases; the first phase was
conducted in August 2018, while the second phase was conducted in March 2019. An
increase (p <0.05) of honey and pollen reserves and the number of adult bees in the
colonies fed with B + supplement was noticed, when the examined parameters were
compared at the end of the first phase. In the second phase, the values of all observed
parameters were significantly higher (p <0.05) in the colonies that used the B+
supplement in their diet, compared to those that did not receive the supplement. In
addition, the number of N. ceranae spores was significantly lower (p <0.05) during
the experiment in colonies fed with B+ supplement, compared to those which were
fed without supplement. Based on the obtained results, positive effects were
determined in colonies that used the tested supplement through their diet, because the
supplement provides nutrients that are not present in pure saccharose syrup. The use
of this supplement in the autumn, prevents the development of microsporidia N.
ceranae, which helps in better wintering of bee colonies. Moreover, application of the
B+ supplement in the spring, provides better preparation of colonies for the main
grazing and improvement of production and reproductive parameters of bee colonies.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
T1  - Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica
T1  - Supplement containing B-complex vitamins can improve bee health and increase colony performance
IS  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3031
ER  - 

@conference{
author = "Jovanović, Nemanja M. and Glavinić, Uroš and Rajković, Milan and Stevanović, Jevrosima and Ristanić, Marko and Ilić, Tamara and Stanimirović, Zoran",
year = "2022",
abstract = "Један од водећих узрока губитака пчелињих заједница је неадекватна исхрана у
погледу квалитета, квантитета и разноврсности, што води повећању осетљивости
на патогене и пестициде. Одузимањем превелике количине меда и прихрана пчела
са чистим шећерним сирупом изазива енергетски и оксидативни стрес што
последично води ка повећаној осетљивости пчела на патогене, првенствено на
ендопаразита Nosema ceranae. У одсуству адекватне хране у пчелињим
заједницама, често се користе суплементи, чиме се може повећати отпорност пчела
према патогенима и одржавати добро кондиционо стање пчелињих заједница. Циљ
овог истраживања је био испитивање ефеката биљног суплемента (комерцијални
назив B+) у кошничким условима, анализирајући параметре јачине пчелињих
заједница (површина отвореног и затвореног легла, резерве меда и полена и број
адултних пчела) и количину ендопаразита N. ceranae. Експеримент је изведен у две
фазе, прва фаза у августу 2018. године и друга фаза у марту 2019. године.
Поређењем праћених параметара на крају прве фазе, уочено је повећање (p < 0,05)
резерви меда и полена и броја адултних пчела у заједницама прихрањиваним
суплементом B+. Током друге фазе, вредности свих посматраних параметара су
биле значајно више (p < 0,05) у заједницама које су у исхрани користиле суплемент
B+ у односу на оне које суплемент нису добијале. Додатно, број спора N. ceranae је
током експеримента био значајно нижи (p < 0,05) у заједницама прихрањиваним
суплементом B+ у односу на оне храњене без додатка суплемента. На основу
добијених резултата утврђени су позитивни ефекти код заједница које су кроз
исхрану користиле тестирани суплемент јер се на тај начин обезбеђују хранљиви
елементи којих нема у чистом шећерном сирупу. Коришћењем овог суплемента у
јесењој прихрани пчела превенира се развој микроспоридије N. ceranae, чиме се
пчелиње заједнице знатно боље зазимљавају. У пролећној прихрани, постиже се
боља припремљеност заједница за главну пашу и побољшање производних и
репродуктивних параметара пчелињих заједница, One of the main causes of bee colony losses is inadequate nutrition in terms of quality,
quantity and diversity, which leads to increased susceptibility to pathogens and
pesticides. Taking too much honey and feeding bees with pure saccharose syrup
causes energetic and oxidative stress, which in turn leads to increased sensitivity of
bees to pathogens, primarily the endoparasite Nosema ceranae. In the absence of
adequate food in bee colonies, supplements are often used, which can increase the
resistance of bees to pathogens and maintain good health condition of bee colonies.
The aim of this study was to examine the effects of herbal supplement (commercial
name B+) in hive conditions, analysing the colonies strength parameters (open and
sealed brood area; honey and pollen reserves and number of adult bees) and level of
N. ceranae. The experiment was conducted in two phases; the first phase was
conducted in August 2018, while the second phase was conducted in March 2019. An
increase (p <0.05) of honey and pollen reserves and the number of adult bees in the
colonies fed with B + supplement was noticed, when the examined parameters were
compared at the end of the first phase. In the second phase, the values of all observed
parameters were significantly higher (p <0.05) in the colonies that used the B+
supplement in their diet, compared to those that did not receive the supplement. In
addition, the number of N. ceranae spores was significantly lower (p <0.05) during
the experiment in colonies fed with B+ supplement, compared to those which were
fed without supplement. Based on the obtained results, positive effects were
determined in colonies that used the tested supplement through their diet, because the
supplement provides nutrients that are not present in pure saccharose syrup. The use
of this supplement in the autumn, prevents the development of microsporidia N.
ceranae, which helps in better wintering of bee colonies. Moreover, application of the
B+ supplement in the spring, provides better preparation of colonies for the main
grazing and improvement of production and reproductive parameters of bee colonies.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022",
title = "Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica, Supplement containing B-complex vitamins can improve bee health and increase colony performance",
number = "140",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3031"
}

Jovanović, N. M., Glavinić, U., Rajković, M., Stevanović, J., Ristanić, M., Ilić, T.,& Stanimirović, Z.. (2022). Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze.(140).
https://hdl.handle.net/21.15107/rcub_veterinar_3031

Jovanović NM, Glavinić U, Rajković M, Stevanović J, Ristanić M, Ilić T, Stanimirović Z. Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022. 2022;(140):null-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3031 .

Jovanović, Nemanja M., Glavinić, Uroš, Rajković, Milan, Stevanović, Jevrosima, Ristanić, Marko, Ilić, Tamara, Stanimirović, Zoran, "Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022, no. 140 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3031 .

TY  - CONF
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja M.
AU  - Blagojević, Jovan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Dominiković, Nina
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3179
AB  - Микроспоридија Nosema ceranae је облигатни интрацелуларни паразит медоносне пчеле (Apis mellifera). Изазива болест ноземозу при чему оставља значајне последице на здравље, репродуктивне и продуктивне способности пчела. Тимол је састојак етеричног уља добијеног од тимијана (Thymus vulgaris) и многих других биљних врста. Користи се у еколошкој контроли пчелињег крпеља Varroa destructor, а у скорије време објављена су и истраживања потенцијалног ефекта тимола у сузбијању Nosema инфекције. У нашем истраживању, код пчела третираних тимолом и инфицираних ноземом праћен је број спора N. ceranae, нивои експресије гена значајних за имунитет пчела као и параметри оксидативног стреса. Тимол је апликован пре инфекције, у тренутку инфицирања, као и три дана након инфекције са N. ceranae. На крају експеримента, тимол конзумиран кроз шећерни сируп показао је антинозематозни ефекат доказан кроз број спора ноземе. Посматрањем нивоа експресије гена кроз време, примећује се да је у готово свим групама које су инфициране ноземом и третиране тимолом, за већину гена, ниво експресије растао. Супресија појединих гена (апидецина, абецин, дефензин) у групи која је примала тимол без инфекције ноземом, говори о потенцијалном имуносупресивном ефекту тимола код неинфицираних пчела. Активности антиоксидативних ензима и концентрације МДА биле су значајно ниже у групама које су током експеримента храњене уз додатак тимола, у односу на инфицирану групу. Наше истраживање доказало је позитивне ефекте тимола на здравље пчела инфицираних ноземом. Наиме, код пчела инфицираних ноземом, нису забележени негативни ефекти апликованог тимола, јер он у овом случају има терапијски (антинозематозни) ефекат, који је далеко значајнији од евентуалних пропратних нежељених дејстава. Међутим, утврдили смо да код пчела без инфекције ноземом, тимол може довести до појединих поремећаја, те треба бити обазрив са прекомерном и неконтролисаном употребом тимола у пчеларству.
AB  - Nosema ceranae is an obligatory intracellular parasite of the honey bee (Apis mellifera). This parasite causes nosemosis, leaving significant consequences on health, reproductive and productive performance of the bees. Thymol is a component of essential oil derived from Thymus vulgaris and many other plant species. It is used in the ecologically-based control of the mite Varroa destructor. Recent investigations indicate the potential effect of thymol in the Nosema control. In our study, the number of N. ceranae spores, gene expression levels (important for bee immunity), as well as the parameters of oxidative stress were monitored in thymol-treated and Nosema-infected bees. Thymol was applied before infection, at the time of infection, as well as three days after infection with N. ceranae. At the end of the experiment, thymol consumed through sugar syrup showed an anti-Nosema effect, proven through the number of Nosema spores. Observation of gene expression levels over time, indicated that in almost all groups infected with Nosema and treated with thymol, levels of expression increased for most of the investigated genes. Suppression of certain genes (apidecin, abecin, defensin) in the group without nosemosis, but treated with thymol, indicates its potential immunosuppressive effect on uninfected bees. The activity of antioxidant enzymes and MDA concentrations were significantly lower in the thymol-fed groups, compared to the infected control group. Our research has proven the positive effects of thymol on the health of Nosema-infected bees. Further on, no negative effects of thymol were observed in infected bees, because in this case it has a therapeutic (anti-Nosema) effect, which is far more significant than potential side effects. However, we found that in non-infected bees, thymol can lead to certain disorders, and one should be careful with excessive and uncontrolled use of thymol in beekeeping.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
T1  - Timol u kontroli nozemoze
T1  - Use of thymol for nosema control
SP  - 138
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3179
ER  - 

@conference{
author = "Glavinić, Uroš and Jovanović, Nemanja M. and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Dominiković, Nina and Stanimirović, Zoran",
year = "2022",
abstract = "Микроспоридија Nosema ceranae је облигатни интрацелуларни паразит медоносне пчеле (Apis mellifera). Изазива болест ноземозу при чему оставља значајне последице на здравље, репродуктивне и продуктивне способности пчела. Тимол је састојак етеричног уља добијеног од тимијана (Thymus vulgaris) и многих других биљних врста. Користи се у еколошкој контроли пчелињег крпеља Varroa destructor, а у скорије време објављена су и истраживања потенцијалног ефекта тимола у сузбијању Nosema инфекције. У нашем истраживању, код пчела третираних тимолом и инфицираних ноземом праћен је број спора N. ceranae, нивои експресије гена значајних за имунитет пчела као и параметри оксидативног стреса. Тимол је апликован пре инфекције, у тренутку инфицирања, као и три дана након инфекције са N. ceranae. На крају експеримента, тимол конзумиран кроз шећерни сируп показао је антинозематозни ефекат доказан кроз број спора ноземе. Посматрањем нивоа експресије гена кроз време, примећује се да је у готово свим групама које су инфициране ноземом и третиране тимолом, за већину гена, ниво експресије растао. Супресија појединих гена (апидецина, абецин, дефензин) у групи која је примала тимол без инфекције ноземом, говори о потенцијалном имуносупресивном ефекту тимола код неинфицираних пчела. Активности антиоксидативних ензима и концентрације МДА биле су значајно ниже у групама које су током експеримента храњене уз додатак тимола, у односу на инфицирану групу. Наше истраживање доказало је позитивне ефекте тимола на здравље пчела инфицираних ноземом. Наиме, код пчела инфицираних ноземом, нису забележени негативни ефекти апликованог тимола, јер он у овом случају има терапијски (антинозематозни) ефекат, који је далеко значајнији од евентуалних пропратних нежељених дејстава. Међутим, утврдили смо да код пчела без инфекције ноземом, тимол може довести до појединих поремећаја, те треба бити обазрив са прекомерном и неконтролисаном употребом тимола у пчеларству., Nosema ceranae is an obligatory intracellular parasite of the honey bee (Apis mellifera). This parasite causes nosemosis, leaving significant consequences on health, reproductive and productive performance of the bees. Thymol is a component of essential oil derived from Thymus vulgaris and many other plant species. It is used in the ecologically-based control of the mite Varroa destructor. Recent investigations indicate the potential effect of thymol in the Nosema control. In our study, the number of N. ceranae spores, gene expression levels (important for bee immunity), as well as the parameters of oxidative stress were monitored in thymol-treated and Nosema-infected bees. Thymol was applied before infection, at the time of infection, as well as three days after infection with N. ceranae. At the end of the experiment, thymol consumed through sugar syrup showed an anti-Nosema effect, proven through the number of Nosema spores. Observation of gene expression levels over time, indicated that in almost all groups infected with Nosema and treated with thymol, levels of expression increased for most of the investigated genes. Suppression of certain genes (apidecin, abecin, defensin) in the group without nosemosis, but treated with thymol, indicates its potential immunosuppressive effect on uninfected bees. The activity of antioxidant enzymes and MDA concentrations were significantly lower in the thymol-fed groups, compared to the infected control group. Our research has proven the positive effects of thymol on the health of Nosema-infected bees. Further on, no negative effects of thymol were observed in infected bees, because in this case it has a therapeutic (anti-Nosema) effect, which is far more significant than potential side effects. However, we found that in non-infected bees, thymol can lead to certain disorders, and one should be careful with excessive and uncontrolled use of thymol in beekeeping.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022",
title = "Timol u kontroli nozemoze, Use of thymol for nosema control",
pages = "138-139",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3179"
}

Glavinić, U., Jovanović, N. M., Blagojević, J., Ristanić, M., Stevanović, J., Dominiković, N.,& Stanimirović, Z.. (2022). Timol u kontroli nozemoze. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 138-139.
https://hdl.handle.net/21.15107/rcub_veterinar_3179

Glavinić U, Jovanović NM, Blagojević J, Ristanić M, Stevanović J, Dominiković N, Stanimirović Z. Timol u kontroli nozemoze. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022. 2022;:138-139.
https://hdl.handle.net/21.15107/rcub_veterinar_3179 .

Glavinić, Uroš, Jovanović, Nemanja M., Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Dominiković, Nina, Stanimirović, Zoran, "Timol u kontroli nozemoze" in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022 (2022):138-139,
https://hdl.handle.net/21.15107/rcub_veterinar_3179 .

TY  - CONF
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jovanović, Nemanja M.
AU  - Rajković, Milan
AU  - Niketić, Mia
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2419
AB  - donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa.
AB  - The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Novi trendovi u kontroli Nosema ceranae
T1  - Advances in Nosema ceranae control
SP  - 79
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2419
ER  - 

@conference{
author = "Glavinić, Uroš and Ristanić, Marko and Jovanović, Nemanja M. and Rajković, Milan and Niketić, Mia and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2022",
abstract = "donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa., The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Novi trendovi u kontroli Nosema ceranae, Advances in Nosema ceranae control",
pages = "79-91",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2419"
}

Glavinić, U., Ristanić, M., Jovanović, N. M., Rajković, M., Niketić, M., Stevanović, J.,& Stanimirović, Z.. (2022). Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419

Glavinić U, Ristanić M, Jovanović NM, Rajković M, Niketić M, Stevanović J, Stanimirović Z. Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

Glavinić, Uroš, Ristanić, Marko, Jovanović, Nemanja M., Rajković, Milan, Niketić, Mia, Stevanović, Jevrosima, Stanimirović, Zoran, "Novi trendovi u kontroli Nosema ceranae" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):79-91,
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Blagojević, Jovan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Mirilović, Milorad
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2467
AB  - Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.
PB  - MDPI
T2  - Insects
T1  - Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees
VL  - 13
IS  - 7
SP  - 574
DO  - 10.3390/insects13070574
ER  - 

@article{
author = "Glavinić, Uroš and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Lakić, Nada and Mirilović, Milorad and Stanimirović, Zoran",
year = "2022",
abstract = "Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.",
publisher = "MDPI",
journal = "Insects",
title = "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees",
volume = "13",
number = "7",
pages = "574",
doi = "10.3390/insects13070574"
}

Glavinić, U., Blagojević, J., Ristanić, M., Stevanović, J., Lakić, N., Mirilović, M.,& Stanimirović, Z.. (2022). Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects
MDPI., 13(7), 574.
https://doi.org/10.3390/insects13070574

Glavinić U, Blagojević J, Ristanić M, Stevanović J, Lakić N, Mirilović M, Stanimirović Z. Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects. 2022;13(7):574.
doi:10.3390/insects13070574 .

Glavinić, Uroš, Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Lakić, Nada, Mirilović, Milorad, Stanimirović, Zoran, "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees" in Insects, 13, no. 7 (2022):574,
https://doi.org/10.3390/insects13070574 . .

TY  - DATA
AU  - Glavinić, Uroš
AU  - Blagojević, Jovan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Lakić, Nada
AU  - Mirilović, Milorad
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2468
AB  - Figure S1. Expression levels of abaecin, hymenoptaecin, defensin, apidaecin and vitellogenin at different time points (day 6, 9 and 15) in experimental groups. N. ceranae infected control (I) and groups infected and supplemented with thymol from day 1 (I-T1), day 3 (I-T3) and day 6 (I-T6)
PB  - MDPI
T2  - Insects
T1  - Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.
VL  - 13
IS  - 7
SP  - 574
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2468
ER  - 

@misc{
author = "Glavinić, Uroš and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Lakić, Nada and Mirilović, Milorad and Stanimirović, Zoran",
year = "2022",
abstract = "Figure S1. Expression levels of abaecin, hymenoptaecin, defensin, apidaecin and vitellogenin at different time points (day 6, 9 and 15) in experimental groups. N. ceranae infected control (I) and groups infected and supplemented with thymol from day 1 (I-T1), day 3 (I-T3) and day 6 (I-T6)",
publisher = "MDPI",
journal = "Insects",
title = "Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.",
volume = "13",
number = "7",
pages = "574",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2468"
}

Glavinić, U., Blagojević, J., Ristanić, M., Stevanović, J., Lakić, N., Mirilović, M.,& Stanimirović, Z.. (2022). Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.. in Insects
MDPI., 13(7), 574.
https://hdl.handle.net/21.15107/rcub_veterinar_2468

Glavinić U, Blagojević J, Ristanić M, Stevanović J, Lakić N, Mirilović M, Stanimirović Z. Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574.. in Insects. 2022;13(7):574.
https://hdl.handle.net/21.15107/rcub_veterinar_2468 .

Glavinić, Uroš, Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Lakić, Nada, Mirilović, Milorad, Stanimirović, Zoran, "Supplementary information for the article: Glavinić, U.; Blagojević, J.; Ristanić, M.; Stevanović, J.; Lakić, N.; Mirilović, M.; Stanimirović, Z. Use of Thymol in Nosema Ceranae Control and Health Improvement of Infected Honey Bees. Insects 2022, 13 (7), 574. https://doi.org/10.3390/insects13070574." in Insects, 13, no. 7 (2022):574,
https://hdl.handle.net/21.15107/rcub_veterinar_2468 .

TY  - DATA
AU  - Brodschneider, Robert
AU  - Schlagbauer, Johannes
AU  - Arakelyan, Iliyana
AU  - Ballis, Alexis
AU  - Brus, Jan
AU  - Brusbardis, Valters
AU  - Cadahía, Luis
AU  - Charrière, Jean-Daniel
AU  - Chlebo, Robert
AU  - Coffey, Mary F.
AU  - Cornelissen, Bram
AU  - da Costa, Cristina Amaro
AU  - Danneels, Ellen
AU  - Danihlík, Jiří
AU  - Dobrescu, Constantin
AU  - Evans, Garth
AU  - Fedoriak, Mariia
AU  - Forsythe, Ivan
AU  - Gregorc, Aleš
AU  - Johannesen, Jes
AU  - Kauko, Lassi
AU  - Kristiansen, Preben
AU  - Martikkala, Maritta
AU  - Martín-Hernández, Raquel
AU  - Mazur, Ewa
AU  - Mutinelli, Franco
AU  - Patalano, Solenn
AU  - Raudmets, Aivar
AU  - Simon Delso, Noa
AU  - Stevanović, Jevrosima
AU  - Uzunov, Aleksandar
AU  - Vejsnæs, Flemming
AU  - Williams, Anthony
AU  - Gray, Alison
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2470
AB  - Table S1. Utilized packages of the statistical software R version 4.0.4.
PB  - Springer
T2  - Journal of Pest Science
T1  - Supplementary information for the article: Brodschneider, R.; Schlagbauer, J.; Arakelyan, I.; Ballis, A.; Brus, J.; Brusbardis, V.; Cadahía, L.; Charrière, J.-D.; Chlebo, R.; Coffey, M. F.; Cornelissen, B.; da Costa, C. A.; Danneels, E.; Danihlík, J.; Dobrescu, C.; Evans, G.; Fedoriak, M.; Forsythe, I.; Gregorc, A.; Johannesen, J.; Kauko, L.; Kristiansen, P.; Martikkala, M.; Martín-Hernández, R.; Mazur, E.; Mutinelli, F.; Patalano, S.; Raudmets, A.; Simon Delso, N.; Stevanovic, J.; Uzunov, A.; Vejsnæs, F.; Williams, A.; Gray, A. Spatial Clusters of Varroa Destructor Control Strategies in Europe. J Pest Sci 2022. https://doi.org/10.1007/s10340-022-01523-2.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2470
ER  - 

@misc{
author = "Brodschneider, Robert and Schlagbauer, Johannes and Arakelyan, Iliyana and Ballis, Alexis and Brus, Jan and Brusbardis, Valters and Cadahía, Luis and Charrière, Jean-Daniel and Chlebo, Robert and Coffey, Mary F. and Cornelissen, Bram and da Costa, Cristina Amaro and Danneels, Ellen and Danihlík, Jiří and Dobrescu, Constantin and Evans, Garth and Fedoriak, Mariia and Forsythe, Ivan and Gregorc, Aleš and Johannesen, Jes and Kauko, Lassi and Kristiansen, Preben and Martikkala, Maritta and Martín-Hernández, Raquel and Mazur, Ewa and Mutinelli, Franco and Patalano, Solenn and Raudmets, Aivar and Simon Delso, Noa and Stevanović, Jevrosima and Uzunov, Aleksandar and Vejsnæs, Flemming and Williams, Anthony and Gray, Alison",
year = "2022",
abstract = "Table S1. Utilized packages of the statistical software R version 4.0.4.",
publisher = "Springer",
journal = "Journal of Pest Science",
title = "Supplementary information for the article: Brodschneider, R.; Schlagbauer, J.; Arakelyan, I.; Ballis, A.; Brus, J.; Brusbardis, V.; Cadahía, L.; Charrière, J.-D.; Chlebo, R.; Coffey, M. F.; Cornelissen, B.; da Costa, C. A.; Danneels, E.; Danihlík, J.; Dobrescu, C.; Evans, G.; Fedoriak, M.; Forsythe, I.; Gregorc, A.; Johannesen, J.; Kauko, L.; Kristiansen, P.; Martikkala, M.; Martín-Hernández, R.; Mazur, E.; Mutinelli, F.; Patalano, S.; Raudmets, A.; Simon Delso, N.; Stevanovic, J.; Uzunov, A.; Vejsnæs, F.; Williams, A.; Gray, A. Spatial Clusters of Varroa Destructor Control Strategies in Europe. J Pest Sci 2022. https://doi.org/10.1007/s10340-022-01523-2.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2470"
}

Brodschneider, R., Schlagbauer, J., Arakelyan, I., Ballis, A., Brus, J., Brusbardis, V., Cadahía, L., Charrière, J., Chlebo, R., Coffey, M. F., Cornelissen, B., da Costa, C. A., Danneels, E., Danihlík, J., Dobrescu, C., Evans, G., Fedoriak, M., Forsythe, I., Gregorc, A., Johannesen, J., Kauko, L., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mazur, E., Mutinelli, F., Patalano, S., Raudmets, A., Simon Delso, N., Stevanović, J., Uzunov, A., Vejsnæs, F., Williams, A.,& Gray, A.. (2022). Supplementary information for the article: Brodschneider, R.; Schlagbauer, J.; Arakelyan, I.; Ballis, A.; Brus, J.; Brusbardis, V.; Cadahía, L.; Charrière, J.-D.; Chlebo, R.; Coffey, M. F.; Cornelissen, B.; da Costa, C. A.; Danneels, E.; Danihlík, J.; Dobrescu, C.; Evans, G.; Fedoriak, M.; Forsythe, I.; Gregorc, A.; Johannesen, J.; Kauko, L.; Kristiansen, P.; Martikkala, M.; Martín-Hernández, R.; Mazur, E.; Mutinelli, F.; Patalano, S.; Raudmets, A.; Simon Delso, N.; Stevanovic, J.; Uzunov, A.; Vejsnæs, F.; Williams, A.; Gray, A. Spatial Clusters of Varroa Destructor Control Strategies in Europe. J Pest Sci 2022. https://doi.org/10.1007/s10340-022-01523-2.. in Journal of Pest Science
Springer..
https://hdl.handle.net/21.15107/rcub_veterinar_2470

Brodschneider R, Schlagbauer J, Arakelyan I, Ballis A, Brus J, Brusbardis V, Cadahía L, Charrière J, Chlebo R, Coffey MF, Cornelissen B, da Costa CA, Danneels E, Danihlík J, Dobrescu C, Evans G, Fedoriak M, Forsythe I, Gregorc A, Johannesen J, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Mazur E, Mutinelli F, Patalano S, Raudmets A, Simon Delso N, Stevanović J, Uzunov A, Vejsnæs F, Williams A, Gray A. Supplementary information for the article: Brodschneider, R.; Schlagbauer, J.; Arakelyan, I.; Ballis, A.; Brus, J.; Brusbardis, V.; Cadahía, L.; Charrière, J.-D.; Chlebo, R.; Coffey, M. F.; Cornelissen, B.; da Costa, C. A.; Danneels, E.; Danihlík, J.; Dobrescu, C.; Evans, G.; Fedoriak, M.; Forsythe, I.; Gregorc, A.; Johannesen, J.; Kauko, L.; Kristiansen, P.; Martikkala, M.; Martín-Hernández, R.; Mazur, E.; Mutinelli, F.; Patalano, S.; Raudmets, A.; Simon Delso, N.; Stevanovic, J.; Uzunov, A.; Vejsnæs, F.; Williams, A.; Gray, A. Spatial Clusters of Varroa Destructor Control Strategies in Europe. J Pest Sci 2022. https://doi.org/10.1007/s10340-022-01523-2.. in Journal of Pest Science. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2470 .

Brodschneider, Robert, Schlagbauer, Johannes, Arakelyan, Iliyana, Ballis, Alexis, Brus, Jan, Brusbardis, Valters, Cadahía, Luis, Charrière, Jean-Daniel, Chlebo, Robert, Coffey, Mary F., Cornelissen, Bram, da Costa, Cristina Amaro, Danneels, Ellen, Danihlík, Jiří, Dobrescu, Constantin, Evans, Garth, Fedoriak, Mariia, Forsythe, Ivan, Gregorc, Aleš, Johannesen, Jes, Kauko, Lassi, Kristiansen, Preben, Martikkala, Maritta, Martín-Hernández, Raquel, Mazur, Ewa, Mutinelli, Franco, Patalano, Solenn, Raudmets, Aivar, Simon Delso, Noa, Stevanović, Jevrosima, Uzunov, Aleksandar, Vejsnæs, Flemming, Williams, Anthony, Gray, Alison, "Supplementary information for the article: Brodschneider, R.; Schlagbauer, J.; Arakelyan, I.; Ballis, A.; Brus, J.; Brusbardis, V.; Cadahía, L.; Charrière, J.-D.; Chlebo, R.; Coffey, M. F.; Cornelissen, B.; da Costa, C. A.; Danneels, E.; Danihlík, J.; Dobrescu, C.; Evans, G.; Fedoriak, M.; Forsythe, I.; Gregorc, A.; Johannesen, J.; Kauko, L.; Kristiansen, P.; Martikkala, M.; Martín-Hernández, R.; Mazur, E.; Mutinelli, F.; Patalano, S.; Raudmets, A.; Simon Delso, N.; Stevanovic, J.; Uzunov, A.; Vejsnæs, F.; Williams, A.; Gray, A. Spatial Clusters of Varroa Destructor Control Strategies in Europe. J Pest Sci 2022. https://doi.org/10.1007/s10340-022-01523-2." in Journal of Pest Science (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2470 .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jelisić, Stefan
AU  - Vejnović, Branislav
AU  - Niketić, Mia
AU  - Stevanović, Jevrosima
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2471
AB  - The hygienic behavior in honey bees is a complex polygenic trait that serves as a natural defense mechanism against bacterial and fungal brood diseases and Varroa destructor mites infesting brood cells. The aim of this study was to evaluate the effect of a dietary amino acids and vitamins supplement “BEEWELL AminoPlus” on hygienic behavior of Apis mellifera colonies combating microsporidial and viral infections. The experiment was performed during a one-year period on 40 colonies alloted to five groups: one supplemented and infected with Nosema ceranae and four viruses (Deformed wing virus - DWV, Acute bee paralysis virus - ABPV, Chronic bee paralysis virus - CBPV and Sacbrood virus – SBV), three not supplemented, but infected with N. ceranae and/ or viruses, and one negative control group. Beside the l isted pathogens, honey bee trypanosomatids were also monitored in all groups.The supplement “BEEWELL AminoPlus” induced a significant and consistent increase of the hygienic behavior in spite of the negative effects of N. ceranae and viral infections. N. ceranae and viruses significantly and consistently decreased hygienic behavior, but also threatened the survival of bee colonies. The tested supplement showed anti-Nosema effect, since the N. ceranae infection level significantly and consistently declined only
PB  - Fakultet veterinarske medicine, Begrad
T2  - Acta Veterinaria
T1  - Diet supplementation helps honey bee colonies in combat infections by enhancing their hygienic behaviour
VL  - 72
IS  - 2
SP  - 145
EP  - 166
DO  - 10.2478/acve-2022-0013
ER  - 

@article{
author = "Stanimirović, Zoran and Glavinić, Uroš and Ristanić, Marko and Jelisić, Stefan and Vejnović, Branislav and Niketić, Mia and Stevanović, Jevrosima",
year = "2022",
abstract = "The hygienic behavior in honey bees is a complex polygenic trait that serves as a natural defense mechanism against bacterial and fungal brood diseases and Varroa destructor mites infesting brood cells. The aim of this study was to evaluate the effect of a dietary amino acids and vitamins supplement “BEEWELL AminoPlus” on hygienic behavior of Apis mellifera colonies combating microsporidial and viral infections. The experiment was performed during a one-year period on 40 colonies alloted to five groups: one supplemented and infected with Nosema ceranae and four viruses (Deformed wing virus - DWV, Acute bee paralysis virus - ABPV, Chronic bee paralysis virus - CBPV and Sacbrood virus – SBV), three not supplemented, but infected with N. ceranae and/ or viruses, and one negative control group. Beside the l isted pathogens, honey bee trypanosomatids were also monitored in all groups.The supplement “BEEWELL AminoPlus” induced a significant and consistent increase of the hygienic behavior in spite of the negative effects of N. ceranae and viral infections. N. ceranae and viruses significantly and consistently decreased hygienic behavior, but also threatened the survival of bee colonies. The tested supplement showed anti-Nosema effect, since the N. ceranae infection level significantly and consistently declined only",
publisher = "Fakultet veterinarske medicine, Begrad",
journal = "Acta Veterinaria",
title = "Diet supplementation helps honey bee colonies in combat infections by enhancing their hygienic behaviour",
volume = "72",
number = "2",
pages = "145-166",
doi = "10.2478/acve-2022-0013"
}

Stanimirović, Z., Glavinić, U., Ristanić, M., Jelisić, S., Vejnović, B., Niketić, M.,& Stevanović, J.. (2022). Diet supplementation helps honey bee colonies in combat infections by enhancing their hygienic behaviour. in Acta Veterinaria
Fakultet veterinarske medicine, Begrad., 72(2), 145-166.
https://doi.org/10.2478/acve-2022-0013

Stanimirović Z, Glavinić U, Ristanić M, Jelisić S, Vejnović B, Niketić M, Stevanović J. Diet supplementation helps honey bee colonies in combat infections by enhancing their hygienic behaviour. in Acta Veterinaria. 2022;72(2):145-166.
doi:10.2478/acve-2022-0013 .

Stanimirović, Zoran, Glavinić, Uroš, Ristanić, Marko, Jelisić, Stefan, Vejnović, Branislav, Niketić, Mia, Stevanović, Jevrosima, "Diet supplementation helps honey bee colonies in combat infections by enhancing their hygienic behaviour" in Acta Veterinaria, 72, no. 2 (2022):145-166,
https://doi.org/10.2478/acve-2022-0013 . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja M.
AU  - Ristanić, Marko
AU  - Milojković-Opsenica, Dušanka
AU  - Mutić, Jelena
AU  - Stevanović, Jevrosima
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2474
AB  - In a cage experiment, lithium chloride (LiCl) and lithium citrate hydrate (Li-cit) were tested for varroacidal efficacy and impact on bees. Treatment with Li-cit (4, 7.5, 10, and 25 mM) resulted in 100% varroacidal efficacy and 100% bee survival. Due to better results in the cage experiment, Li-cit was further tested in field experiments on full-sized free-flying colonies treated three times in 6-day intervals. All the concentrations of Li-cit in the field experiment (5, 10, 15, 20, and 25 mM) expressed high varroacidal efficacy: 93.2–95.5%, significantly (p < 0.01) greater than in the negative and positive (amitraz-treated) controls. Lithium residues in honey from brood chambers were much higher nine months after the last treatment (169.3–1756.0 μg/kg) than seven days post-treatment (19.2–27.8 μg/kg). In honey from honey chambers (eligible for human consumption), the average lithium residues were 26.9 μg/kg and 33.7 μg/kg seven days after the last treatment. In wax combs taken from the brood chamber nine months post-treatment, lithium residues ranged from 410 μg/kg to 2314 µg/kg, without significant differences from the negative control. Lithium residues in wax matrices seven days after the last treatment were in a narrow range of 234.3–300 µg/kg, in wax combs and cappings being significantly lower than in commercial wax foundations. For the first time, Li-cit proved to be effective against Varroa destructor under field conditions.
PB  - International Bee Research Association
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices
VL  - 61
IS  - 3
SP  - 375
EP  - 391
DO  - 10.1080/00218839.2021.1988277
ER  - 

@article{
author = "Stanimirović, Zoran and Glavinić, Uroš and Jovanović, Nemanja M. and Ristanić, Marko and Milojković-Opsenica, Dušanka and Mutić, Jelena and Stevanović, Jevrosima",
year = "2022",
abstract = "In a cage experiment, lithium chloride (LiCl) and lithium citrate hydrate (Li-cit) were tested for varroacidal efficacy and impact on bees. Treatment with Li-cit (4, 7.5, 10, and 25 mM) resulted in 100% varroacidal efficacy and 100% bee survival. Due to better results in the cage experiment, Li-cit was further tested in field experiments on full-sized free-flying colonies treated three times in 6-day intervals. All the concentrations of Li-cit in the field experiment (5, 10, 15, 20, and 25 mM) expressed high varroacidal efficacy: 93.2–95.5%, significantly (p < 0.01) greater than in the negative and positive (amitraz-treated) controls. Lithium residues in honey from brood chambers were much higher nine months after the last treatment (169.3–1756.0 μg/kg) than seven days post-treatment (19.2–27.8 μg/kg). In honey from honey chambers (eligible for human consumption), the average lithium residues were 26.9 μg/kg and 33.7 μg/kg seven days after the last treatment. In wax combs taken from the brood chamber nine months post-treatment, lithium residues ranged from 410 μg/kg to 2314 µg/kg, without significant differences from the negative control. Lithium residues in wax matrices seven days after the last treatment were in a narrow range of 234.3–300 µg/kg, in wax combs and cappings being significantly lower than in commercial wax foundations. For the first time, Li-cit proved to be effective against Varroa destructor under field conditions.",
publisher = "International Bee Research Association, Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices",
volume = "61",
number = "3",
pages = "375-391",
doi = "10.1080/00218839.2021.1988277"
}

Stanimirović, Z., Glavinić, U., Jovanović, N. M., Ristanić, M., Milojković-Opsenica, D., Mutić, J.,& Stevanović, J.. (2022). Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices. in Journal of Apicultural Research
International Bee Research Association., 61(3), 375-391.
https://doi.org/10.1080/00218839.2021.1988277

Stanimirović Z, Glavinić U, Jovanović NM, Ristanić M, Milojković-Opsenica D, Mutić J, Stevanović J. Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices. in Journal of Apicultural Research. 2022;61(3):375-391.
doi:10.1080/00218839.2021.1988277 .

Stanimirović, Zoran, Glavinić, Uroš, Jovanović, Nemanja M., Ristanić, Marko, Milojković-Opsenica, Dušanka, Mutić, Jelena, Stevanović, Jevrosima, "Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices" in Journal of Apicultural Research, 61, no. 3 (2022):375-391,
https://doi.org/10.1080/00218839.2021.1988277 . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Glavinić, Uroš
AU  - Jovanović, Nemanja M.
AU  - Ristanić, Marko
AU  - Milojković-Opsenica, Dušanka
AU  - Mutić, Jelena
AU  - Stevanović, Jevrosima
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2475
AB  - In a cage experiment, lithium chloride (LiCl) and lithium citrate hydrate (Li-cit) were tested for varroacidal efficacy and impact on bees. Treatment with Li-cit (4, 7.5, 10, and 25 mM) resulted in 100% varroacidal efficacy and 100% bee survival. Due to better results in the cage experiment, Li-cit was further tested in field experiments on full-sized free-flying colonies treated three times in 6-day intervals. All the concentrations of Li-cit in the field experiment (5, 10, 15, 20, and 25 mM) expressed high varroacidal efficacy: 93.2–95.5%, significantly (p < 0.01) greater than in the negative and positive (amitraz-treated) controls. Lithium residues in honey from brood chambers were much higher nine months after the last treatment (169.3–1756.0 μg/kg) than seven days post-treatment (19.2–27.8 μg/kg). In honey from honey chambers (eligible for human consumption), the average lithium residues were 26.9 μg/kg and 33.7 μg/kg seven days after the last treatment. In wax combs taken from the brood chamber nine months post-treatment, lithium residues ranged from 410 μg/kg to 2314 µg/kg, without significant differences from the negative control. Lithium residues in wax matrices seven days after the last treatment were in a narrow range of 234.3–300 µg/kg, in wax combs and cappings being significantly lower than in commercial wax foundations. For the first time, Li-cit proved to be effective against Varroa destructor under field conditions.
PB  - International Bee Research Association
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Supplementary information for the article: Stanimirovic, Z.; Glavinic, U.; Jovanovic, N. M.; Ristanic, M.; Milojković-Opsenica, D.; Mutic, J.; Stevanovic, J. Preliminary Trials on Effects of Lithium Salts on Varroa Destructor, Honey and Wax Matrices. Journal of Apicultural Research 2022, 61 (3), 375–391. https://doi.org/10.1080/00218839.2021.1988277.
VL  - 61
IS  - 3
SP  - 375
EP  - 391
DO  - 10.1080/00218839.2021.1988277
ER  - 

@article{
author = "Stanimirović, Zoran and Glavinić, Uroš and Jovanović, Nemanja M. and Ristanić, Marko and Milojković-Opsenica, Dušanka and Mutić, Jelena and Stevanović, Jevrosima",
year = "2022",
abstract = "In a cage experiment, lithium chloride (LiCl) and lithium citrate hydrate (Li-cit) were tested for varroacidal efficacy and impact on bees. Treatment with Li-cit (4, 7.5, 10, and 25 mM) resulted in 100% varroacidal efficacy and 100% bee survival. Due to better results in the cage experiment, Li-cit was further tested in field experiments on full-sized free-flying colonies treated three times in 6-day intervals. All the concentrations of Li-cit in the field experiment (5, 10, 15, 20, and 25 mM) expressed high varroacidal efficacy: 93.2–95.5%, significantly (p < 0.01) greater than in the negative and positive (amitraz-treated) controls. Lithium residues in honey from brood chambers were much higher nine months after the last treatment (169.3–1756.0 μg/kg) than seven days post-treatment (19.2–27.8 μg/kg). In honey from honey chambers (eligible for human consumption), the average lithium residues were 26.9 μg/kg and 33.7 μg/kg seven days after the last treatment. In wax combs taken from the brood chamber nine months post-treatment, lithium residues ranged from 410 μg/kg to 2314 µg/kg, without significant differences from the negative control. Lithium residues in wax matrices seven days after the last treatment were in a narrow range of 234.3–300 µg/kg, in wax combs and cappings being significantly lower than in commercial wax foundations. For the first time, Li-cit proved to be effective against Varroa destructor under field conditions.",
publisher = "International Bee Research Association, Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Supplementary information for the article: Stanimirovic, Z.; Glavinic, U.; Jovanovic, N. M.; Ristanic, M.; Milojković-Opsenica, D.; Mutic, J.; Stevanovic, J. Preliminary Trials on Effects of Lithium Salts on Varroa Destructor, Honey and Wax Matrices. Journal of Apicultural Research 2022, 61 (3), 375–391. https://doi.org/10.1080/00218839.2021.1988277.",
volume = "61",
number = "3",
pages = "375-391",
doi = "10.1080/00218839.2021.1988277"
}

Stanimirović, Z., Glavinić, U., Jovanović, N. M., Ristanić, M., Milojković-Opsenica, D., Mutić, J.,& Stevanović, J.. (2022). Supplementary information for the article: Stanimirovic, Z.; Glavinic, U.; Jovanovic, N. M.; Ristanic, M.; Milojković-Opsenica, D.; Mutic, J.; Stevanovic, J. Preliminary Trials on Effects of Lithium Salts on Varroa Destructor, Honey and Wax Matrices. Journal of Apicultural Research 2022, 61 (3), 375–391. https://doi.org/10.1080/00218839.2021.1988277.. in Journal of Apicultural Research
International Bee Research Association., 61(3), 375-391.
https://doi.org/10.1080/00218839.2021.1988277

Stanimirović Z, Glavinić U, Jovanović NM, Ristanić M, Milojković-Opsenica D, Mutić J, Stevanović J. Supplementary information for the article: Stanimirovic, Z.; Glavinic, U.; Jovanovic, N. M.; Ristanic, M.; Milojković-Opsenica, D.; Mutic, J.; Stevanovic, J. Preliminary Trials on Effects of Lithium Salts on Varroa Destructor, Honey and Wax Matrices. Journal of Apicultural Research 2022, 61 (3), 375–391. https://doi.org/10.1080/00218839.2021.1988277.. in Journal of Apicultural Research. 2022;61(3):375-391.
doi:10.1080/00218839.2021.1988277 .

Stanimirović, Zoran, Glavinić, Uroš, Jovanović, Nemanja M., Ristanić, Marko, Milojković-Opsenica, Dušanka, Mutić, Jelena, Stevanović, Jevrosima, "Supplementary information for the article: Stanimirovic, Z.; Glavinic, U.; Jovanovic, N. M.; Ristanic, M.; Milojković-Opsenica, D.; Mutic, J.; Stevanovic, J. Preliminary Trials on Effects of Lithium Salts on Varroa Destructor, Honey and Wax Matrices. Journal of Apicultural Research 2022, 61 (3), 375–391. https://doi.org/10.1080/00218839.2021.1988277." in Journal of Apicultural Research, 61, no. 3 (2022):375-391,
https://doi.org/10.1080/00218839.2021.1988277 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Vejnović, Branislav
AU  - Goblirsch, Michael
AU  - Glavinić, Uroš
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2502
AB  - Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.
PB  - Taylor & Francis
T2  - Journal of Apicultural Research
T2  - Journal of Apicultural Research
T1  - Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay
DO  - 10.1080/00218839.2022.2146367
ER  - 

@article{
author = "Rajković, Milan and Stanimirović, Zoran and Stevanović, Jevrosima and Ristanić, Marko and Vejnović, Branislav and Goblirsch, Michael and Glavinić, Uroš",
year = "2022",
abstract = "Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.",
publisher = "Taylor & Francis",
journal = "Journal of Apicultural Research, Journal of Apicultural Research",
title = "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay",
doi = "10.1080/00218839.2022.2146367"
}

Rajković, M., Stanimirović, Z., Stevanović, J., Ristanić, M., Vejnović, B., Goblirsch, M.,& Glavinić, U.. (2022). Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research
Taylor & Francis..
https://doi.org/10.1080/00218839.2022.2146367

Rajković M, Stanimirović Z, Stevanović J, Ristanić M, Vejnović B, Goblirsch M, Glavinić U. Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research. 2022;.
doi:10.1080/00218839.2022.2146367 .

Rajković, Milan, Stanimirović, Zoran, Stevanović, Jevrosima, Ristanić, Marko, Vejnović, Branislav, Goblirsch, Michael, Glavinić, Uroš, "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay" in Journal of Apicultural Research (2022),
https://doi.org/10.1080/00218839.2022.2146367 . .

TY  - JOUR
AU  - Jovanović, Nemanja M.
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Vejnović, Branislav
AU  - Stevanović, Jevrosima
AU  - Ćosić, Milivoje
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2503
AB  - With an almost global distribution, Varroa destuctor is the leading cause of weakening and loss of honey bee colonies. New substances are constantly being tested in order to find those that will exhibit high anti-Varroa efficacy at low doses/concentrations, without unwanted effects on bees. Lithium (Li) salts stood out as candidates based on previous research. The aims of this study were to evaluate Li citrate hydrate (Li-cit) for its contact efficacy against Varroa, but also the effect of Li-cit on honey bees by estimating loads of honey bee viruses, expression levels of immune-related genes and genes for antioxidative enzymes and oxidative stress parameters on two sampling occasions, before the treatment and after the treatment. Our experiment was performed on four groups, each consisting of seven colonies. Two groups were treated with the test compound, one receiving 5 mM and the other 10 mM of Li-cit; the third received oxalic acid treatment (OA group) and served as positive control, and the fourth was negative control (C group), treated with 50% w/v pure sucrose-water syrup. Single trickling treatment was applied in all groups. Both tested concentrations of Li-cit, 5 and 10 mM, expressed high varroacidal efficacy, 96.85% and 96.80%, respectively. Load of Chronic Bee Paralysis Virus significantly decreased (p < 0.01) after the treatment in group treated with 5 mM of Li-cit. In OA group, loads of Acute Bee Paralysis Virus and Deformed Wing Virus significantly (p < 0.05) increased, and in C group, loads of all viruses significantly (p < 0.01 or p < 0.001) increased. Transcript levels of genes for abaecin, apidaecin, defensin and vitellogenin were significantly higher (p < 0.05—p < 0.001), while all oxidative stress parameters were significantly lower (p < 0.05—p < 0.001) after the treatment in both groups treated with Li-cit. All presented results along with easy application indicate benefits of topical Li-cit treatment and complete the mosaic of evidence on the advantages of this salt in the control of Varroa.
PB  - Frontiers Media
T2  - Frontiers in Physiology
T2  - Frontiers in Physiology
T1  - Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment
DO  - 10.3389/fphys.2022.1000944
ER  - 

@article{
author = "Jovanović, Nemanja M. and Glavinić, Uroš and Ristanić, Marko and Vejnović, Branislav and Stevanović, Jevrosima and Ćosić, Milivoje and Stanimirović, Zoran",
year = "2022",
abstract = "With an almost global distribution, Varroa destuctor is the leading cause of weakening and loss of honey bee colonies. New substances are constantly being tested in order to find those that will exhibit high anti-Varroa efficacy at low doses/concentrations, without unwanted effects on bees. Lithium (Li) salts stood out as candidates based on previous research. The aims of this study were to evaluate Li citrate hydrate (Li-cit) for its contact efficacy against Varroa, but also the effect of Li-cit on honey bees by estimating loads of honey bee viruses, expression levels of immune-related genes and genes for antioxidative enzymes and oxidative stress parameters on two sampling occasions, before the treatment and after the treatment. Our experiment was performed on four groups, each consisting of seven colonies. Two groups were treated with the test compound, one receiving 5 mM and the other 10 mM of Li-cit; the third received oxalic acid treatment (OA group) and served as positive control, and the fourth was negative control (C group), treated with 50% w/v pure sucrose-water syrup. Single trickling treatment was applied in all groups. Both tested concentrations of Li-cit, 5 and 10 mM, expressed high varroacidal efficacy, 96.85% and 96.80%, respectively. Load of Chronic Bee Paralysis Virus significantly decreased (p < 0.01) after the treatment in group treated with 5 mM of Li-cit. In OA group, loads of Acute Bee Paralysis Virus and Deformed Wing Virus significantly (p < 0.05) increased, and in C group, loads of all viruses significantly (p < 0.01 or p < 0.001) increased. Transcript levels of genes for abaecin, apidaecin, defensin and vitellogenin were significantly higher (p < 0.05—p < 0.001), while all oxidative stress parameters were significantly lower (p < 0.05—p < 0.001) after the treatment in both groups treated with Li-cit. All presented results along with easy application indicate benefits of topical Li-cit treatment and complete the mosaic of evidence on the advantages of this salt in the control of Varroa.",
publisher = "Frontiers Media",
journal = "Frontiers in Physiology, Frontiers in Physiology",
title = "Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment",
doi = "10.3389/fphys.2022.1000944"
}

Jovanović, N. M., Glavinić, U., Ristanić, M., Vejnović, B., Stevanović, J., Ćosić, M.,& Stanimirović, Z.. (2022). Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment. in Frontiers in Physiology
Frontiers Media..
https://doi.org/10.3389/fphys.2022.1000944

Jovanović NM, Glavinić U, Ristanić M, Vejnović B, Stevanović J, Ćosić M, Stanimirović Z. Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment. in Frontiers in Physiology. 2022;.
doi:10.3389/fphys.2022.1000944 .

Jovanović, Nemanja M., Glavinić, Uroš, Ristanić, Marko, Vejnović, Branislav, Stevanović, Jevrosima, Ćosić, Milivoje, Stanimirović, Zoran, "Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment" in Frontiers in Physiology (2022),
https://doi.org/10.3389/fphys.2022.1000944 . .