TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Stevančević, Ognjen
AU  - Kureljušić, Branislav
AU  - Nešić, Ksenija
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1990
AB  - Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.
PB  - Springer
T2  - Archives of Virology
T1  - Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia
VL  - 165
IS  - 1
SP  - 193
EP  - 199
DO  - 10.1007/s00705-019-04455-y
ER  - 

@article{
author = "Savić, Božidar and Milićević, Vesna and Radanović, Oliver and Zdravković, Nemanja and Stevančević, Ognjen and Kureljušić, Branislav and Nešić, Ksenija",
year = "2020",
abstract = "Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.",
publisher = "Springer",
journal = "Archives of Virology",
title = "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia",
volume = "165",
number = "1",
pages = "193-199",
doi = "10.1007/s00705-019-04455-y"
}

Savić, B., Milićević, V., Radanović, O., Zdravković, N., Stevančević, O., Kureljušić, B.,& Nešić, K.. (2020). Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology
Springer., 165(1), 193-199.
https://doi.org/10.1007/s00705-019-04455-y

Savić B, Milićević V, Radanović O, Zdravković N, Stevančević O, Kureljušić B, Nešić K. Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology. 2020;165(1):193-199.
doi:10.1007/s00705-019-04455-y .

Savić, Božidar, Milićević, Vesna, Radanović, Oliver, Zdravković, Nemanja, Stevančević, Ognjen, Kureljušić, Branislav, Nešić, Ksenija, "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia" in Archives of Virology, 165, no. 1 (2020):193-199,
https://doi.org/10.1007/s00705-019-04455-y . .

TY  - JOUR
AU  - Đoković, Radojica
AU  - Dosković, Vladimir
AU  - Cincović, Marko
AU  - Belić, Branislava
AU  - Fratrić, Natalija
AU  - Jasović, Boban
AU  - Lalović, Miroslav
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1547
AB  - The objective of this study was to determine insulin resistance in healthy (n=8) and ketotic (n=7) dairy cows based on the difference between basal and dynamic changes in glucose, insulin, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB) concentrations, and on the determination of their mutual relationship and relationship with insulin resistance indices (RQUICKI and RQUICKI-BHB), after intravenous glucose tolerance test (IVGTT). The RQUICKI index showed a significant linear correlation (P  lt  0.05) with basal insulin, glucose, NEFA and BHB levels. However, RQUICKI-BHB index values exhibited a negative correlation (P  lt  0.01) with basal NEFA and BHB values and their clearance rates, as well as with glucose clearance rate, and a positive correlation (P  lt  0.05) with basal insulin values as well as with insulin and glucose responses during IVGTT. The correlation between basal values of these parameters and the values measured or calculated during IVGTT is the result of RQUICKI-BHB values, as the exclusion of RQUICKI-BHB leads to loss of the statistical significance of the correlations between basal and dynamic values. Insulin resistance in ketotic cows is characterized by decreased insulin response to glucose and increased insulin resistance of the tissue, their correlation being the result of the RQUICKI-BHB index value.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test
VL  - 37
IS  - 4
SP  - 387
EP  - 392
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1547
ER  - 

@article{
author = "Đoković, Radojica and Dosković, Vladimir and Cincović, Marko and Belić, Branislava and Fratrić, Natalija and Jasović, Boban and Lalović, Miroslav",
year = "2017",
abstract = "The objective of this study was to determine insulin resistance in healthy (n=8) and ketotic (n=7) dairy cows based on the difference between basal and dynamic changes in glucose, insulin, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB) concentrations, and on the determination of their mutual relationship and relationship with insulin resistance indices (RQUICKI and RQUICKI-BHB), after intravenous glucose tolerance test (IVGTT). The RQUICKI index showed a significant linear correlation (P  lt  0.05) with basal insulin, glucose, NEFA and BHB levels. However, RQUICKI-BHB index values exhibited a negative correlation (P  lt  0.01) with basal NEFA and BHB values and their clearance rates, as well as with glucose clearance rate, and a positive correlation (P  lt  0.05) with basal insulin values as well as with insulin and glucose responses during IVGTT. The correlation between basal values of these parameters and the values measured or calculated during IVGTT is the result of RQUICKI-BHB values, as the exclusion of RQUICKI-BHB leads to loss of the statistical significance of the correlations between basal and dynamic values. Insulin resistance in ketotic cows is characterized by decreased insulin response to glucose and increased insulin resistance of the tissue, their correlation being the result of the RQUICKI-BHB index value.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test",
volume = "37",
number = "4",
pages = "387-392",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1547"
}

Đoković, R., Dosković, V., Cincović, M., Belić, B., Fratrić, N., Jasović, B.,& Lalović, M.. (2017). Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 37(4), 387-392.
https://hdl.handle.net/21.15107/rcub_veterinar_1547

Đoković R, Dosković V, Cincović M, Belić B, Fratrić N, Jasović B, Lalović M. Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test. in Pakistan Veterinary Journal. 2017;37(4):387-392.
https://hdl.handle.net/21.15107/rcub_veterinar_1547 .

Đoković, Radojica, Dosković, Vladimir, Cincović, Marko, Belić, Branislava, Fratrić, Natalija, Jasović, Boban, Lalović, Miroslav, "Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test" in Pakistan Veterinary Journal, 37, no. 4 (2017):387-392,
https://hdl.handle.net/21.15107/rcub_veterinar_1547 .

TY  - JOUR
AU  - Cincović, Marko
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Belić, Branislava
AU  - Đoković, Radojica
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1511
AB  - Insulin resistance is a phenomenon which accompanies the ongoing metabolic adaptation in cows during early lactation. The aim of our study was to determine the linear correlations of HOMA (Homeostatic Model Assessment), QUICKI (Quantitative Insulin Sensitivity Check Index) and RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) indexes of insulin resistance with the metabolic status of cows (concentration of hormones, metabolites and body condition score). The experiment included 40 Holstein-Frisian cows in the first week after calving. Indexes of insulin resistance valued: 18.68 +/- 5.43 (HOMA), 0.39 +/- 0.06 (QUICKI) and 0.45 +/- 0.06 (RQUICKI). Linear correlations were examined by testing the coefficient of correlation (r), determination (r(2),%) and regression parameter beta (b) in linear equation. A negative correlation was found between HOMA and IGF-I (insulin growth factor I) (r=-0.51, r(2)=25.0, b=-1.1257, p<0.01). HOMA showed a positive correlation with BHB (beta-hidroxybutyrate) (r=0.48, r(2)=23.2, b=0.0234, p<0.01). A positive correlation was found between QUICKI and IGF-I (r=0.30, r(2)=10.0 b=46.7900, p<0.05) and cholesterol (r=0.44, r(2)=18.3, b=1.9021, p<0.01). In contrast, QUICKI and BHB (r=0.51, r(2)=27.1, b=-1.7241, p<0.01), just like QUICKI and BCS (r=0.46, r(2)=20.9, b=-2.424, p<0.01), showed a negative correlation. RQUICKI showed positive correlations with IGF-I (r=0.48, r(2)=22.8, b=28.1230, p<0.01), T4 (r=0.47, r(2)=22.1, b=87.142, p<0.01) and triglycerides (r=0.36, r(2)=13, b=0.0407, p<0.05) but negative correlations with cortisol (r=-0.36, r(2)=13.0, b=-9.0332, p<0.05), STH (somatotropic hormone) (r=-0.42, r(2)=17.3, b=-5.4976, p<0.01), BHB (r=-0.62, r(2)=38.3, b=-1.1872, p<0.01), total bilirubin (r=0.58, r(2)=33.7, b=-7.131, p<0.01) and BCS (body condition score) (r=-0.6, r(2)=36.4, b=1.8347, p<0.01). In conclusion, indexes of insulin resistance may be used to evaluate the metabolic status of cows in early lactation. RQUICKI might be the most appropriate predictor of metabolic status due to its linear relationship with most of the parameters included in homeorhetic process.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation
VL  - 67
IS  - 1
SP  - 57
EP  - 70
DO  - 10.1515/acve-2017-0006
ER  - 

@article{
author = "Cincović, Marko and Kirovski, Danijela and Vujanac, Ivan and Belić, Branislava and Đoković, Radojica",
year = "2017",
abstract = "Insulin resistance is a phenomenon which accompanies the ongoing metabolic adaptation in cows during early lactation. The aim of our study was to determine the linear correlations of HOMA (Homeostatic Model Assessment), QUICKI (Quantitative Insulin Sensitivity Check Index) and RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) indexes of insulin resistance with the metabolic status of cows (concentration of hormones, metabolites and body condition score). The experiment included 40 Holstein-Frisian cows in the first week after calving. Indexes of insulin resistance valued: 18.68 +/- 5.43 (HOMA), 0.39 +/- 0.06 (QUICKI) and 0.45 +/- 0.06 (RQUICKI). Linear correlations were examined by testing the coefficient of correlation (r), determination (r(2),%) and regression parameter beta (b) in linear equation. A negative correlation was found between HOMA and IGF-I (insulin growth factor I) (r=-0.51, r(2)=25.0, b=-1.1257, p<0.01). HOMA showed a positive correlation with BHB (beta-hidroxybutyrate) (r=0.48, r(2)=23.2, b=0.0234, p<0.01). A positive correlation was found between QUICKI and IGF-I (r=0.30, r(2)=10.0 b=46.7900, p<0.05) and cholesterol (r=0.44, r(2)=18.3, b=1.9021, p<0.01). In contrast, QUICKI and BHB (r=0.51, r(2)=27.1, b=-1.7241, p<0.01), just like QUICKI and BCS (r=0.46, r(2)=20.9, b=-2.424, p<0.01), showed a negative correlation. RQUICKI showed positive correlations with IGF-I (r=0.48, r(2)=22.8, b=28.1230, p<0.01), T4 (r=0.47, r(2)=22.1, b=87.142, p<0.01) and triglycerides (r=0.36, r(2)=13, b=0.0407, p<0.05) but negative correlations with cortisol (r=-0.36, r(2)=13.0, b=-9.0332, p<0.05), STH (somatotropic hormone) (r=-0.42, r(2)=17.3, b=-5.4976, p<0.01), BHB (r=-0.62, r(2)=38.3, b=-1.1872, p<0.01), total bilirubin (r=0.58, r(2)=33.7, b=-7.131, p<0.01) and BCS (body condition score) (r=-0.6, r(2)=36.4, b=1.8347, p<0.01). In conclusion, indexes of insulin resistance may be used to evaluate the metabolic status of cows in early lactation. RQUICKI might be the most appropriate predictor of metabolic status due to its linear relationship with most of the parameters included in homeorhetic process.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation",
volume = "67",
number = "1",
pages = "57-70",
doi = "10.1515/acve-2017-0006"
}

Cincović, M., Kirovski, D., Vujanac, I., Belić, B.,& Đoković, R.. (2017). Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 57-70.
https://doi.org/10.1515/acve-2017-0006

Cincović M, Kirovski D, Vujanac I, Belić B, Đoković R. Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation. in Acta Veterinaria-Beograd. 2017;67(1):57-70.
doi:10.1515/acve-2017-0006 .

Cincović, Marko, Kirovski, Danijela, Vujanac, Ivan, Belić, Branislava, Đoković, Radojica, "Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation" in Acta Veterinaria-Beograd, 67, no. 1 (2017):57-70,
https://doi.org/10.1515/acve-2017-0006 . .

TY  - JOUR
AU  - Cvetojević, D.
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Jakić-Dimić, Dobrila
AU  - Pavlović, Miloš
AU  - Spalević, Ljiljana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1416
AB  - Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. In this study a retrospective PCR examination of 100 extracted DNA samples from aborting cows was performed in order to determine: prevalence of BHV-4 in abortive cattle, whether coinfections BHV-4 with other abortifacient pathogens are present in the same sample and to determine the month of gestation when BHV-4 associated abortions were detected. Out of 100 examined samples, the BHV-4 genome was detected in 21 samples (21%). In two samples we detected coinfection of BHV-4 with bovine viral diarrhea virus (BVDV) and in one with Neospora caninum. Most of the BHV-4-associated abortions were detected during the seventh month of gestation. It was concluded that an active BHV-4 infection was present among cows that aborted on the farms examined. The high prevalence of the BHV-4 genome in abortion material suggests that this virus may have cause the abortions. Further studies and examinations are needed to establish causative connection between presence of BHV-4 and abortion.
PB  - Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa
T2  - Polish Journal of Veterinary Sciences
T1  - Prevalence of Bovine herpesvirus type 4 in aborting dairy cows
VL  - 19
IS  - 4
SP  - 731
EP  - 736
DO  - 10.1515/pjvs-2016-0092
ER  - 

@article{
author = "Cvetojević, D. and Savić, Božidar and Milićević, Vesna and Kureljušić, Branislav and Jezdimirović, Nemanja and Jakić-Dimić, Dobrila and Pavlović, Miloš and Spalević, Ljiljana",
year = "2016",
abstract = "Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. In this study a retrospective PCR examination of 100 extracted DNA samples from aborting cows was performed in order to determine: prevalence of BHV-4 in abortive cattle, whether coinfections BHV-4 with other abortifacient pathogens are present in the same sample and to determine the month of gestation when BHV-4 associated abortions were detected. Out of 100 examined samples, the BHV-4 genome was detected in 21 samples (21%). In two samples we detected coinfection of BHV-4 with bovine viral diarrhea virus (BVDV) and in one with Neospora caninum. Most of the BHV-4-associated abortions were detected during the seventh month of gestation. It was concluded that an active BHV-4 infection was present among cows that aborted on the farms examined. The high prevalence of the BHV-4 genome in abortion material suggests that this virus may have cause the abortions. Further studies and examinations are needed to establish causative connection between presence of BHV-4 and abortion.",
publisher = "Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa",
journal = "Polish Journal of Veterinary Sciences",
title = "Prevalence of Bovine herpesvirus type 4 in aborting dairy cows",
volume = "19",
number = "4",
pages = "731-736",
doi = "10.1515/pjvs-2016-0092"
}

Cvetojević, D., Savić, B., Milićević, V., Kureljušić, B., Jezdimirović, N., Jakić-Dimić, D., Pavlović, M.,& Spalević, L.. (2016). Prevalence of Bovine herpesvirus type 4 in aborting dairy cows. in Polish Journal of Veterinary Sciences
Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, Warszawa., 19(4), 731-736.
https://doi.org/10.1515/pjvs-2016-0092

Cvetojević D, Savić B, Milićević V, Kureljušić B, Jezdimirović N, Jakić-Dimić D, Pavlović M, Spalević L. Prevalence of Bovine herpesvirus type 4 in aborting dairy cows. in Polish Journal of Veterinary Sciences. 2016;19(4):731-736.
doi:10.1515/pjvs-2016-0092 .

Cvetojević, D., Savić, Božidar, Milićević, Vesna, Kureljušić, Branislav, Jezdimirović, Nemanja, Jakić-Dimić, Dobrila, Pavlović, Miloš, Spalević, Ljiljana, "Prevalence of Bovine herpesvirus type 4 in aborting dairy cows" in Polish Journal of Veterinary Sciences, 19, no. 4 (2016):731-736,
https://doi.org/10.1515/pjvs-2016-0092 . .

TY  - JOUR
AU  - Krsmanović, Milorad
AU  - Đoković, Radojica
AU  - Cincović, Marko
AU  - Ostojić-Andrić, Dušica
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1319
AB  - This study examined the activities of aspartate-aminotransferase (AST), gamma-glutamyl-transferase (GGT) and lactate-dehydrogenase (LDH) in the blood serum of 45 dairy Simmental cows divided into three groups according to production period. The first group (n=15) consisted of late pregnant dairy cows, the second group (n=15) cows in the early lactation, and the third group (n=15) cow in mid lactation. The significant higher activity (P lt 0.05) of AST, GGT and LDH were determined in the early lactation period than in dry period and during full lactation. Research results showed possibility of mild degree of hepatic lesions, probably due to fat infiltration in early lactation cows. Serum AST enzyme activities were significant correlated (P lt 0.05) with GGT and LDH activities and may be most sensitive indicator.
AB  - U ovom radu je određivana aktivnost aspartat-aminotransferaze (AST), gama-glutamat-transferaze (GGT) i laktat dehidrogenaze (LDH) u krvnom serumu kod 45 simentalskih mlečnih krava, podeljenih u tri grupe u zavisnosti od produktivnog perioda. Prvu grupu (n=15) su činile visoko gravidne krave, drugu grupu (n=15) krave u ranoj laktaciji, a treću grupu (n=15) krave tokom pune laktacije. Statistički značajno veće aktivnosti AST (P lt 0.05),GGT (P lt 0.05) i LDH (P lt 0.05) u krvnom serumu su utvrđene kod krava u ranoj laktaciji u odnosu na aktivnosti ovih enzima u serumu kod zasušenih krava i krava u punoj laktaciji. Dobijeni rezultati ukazuju na mogućnost blagog stepena oštećenja ćelija jetre, odnosno masnu infiltraciju hepatocita kod krava na početku laktacije. Serumske aktivnosti AST su bile u značajnoj korelaciji (P lt 0.05) sa aktivnostima GGT i LDH u krvnom serumu pa se AST može smatrati pouzdanim indikatorom u tvrđivanju funkcionalnog stanja jetre kod mlečnih krava tokom peripartalnog perioda i pune laktacije.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations
T1  - Određivanje aktivnosti specifičnih enzima krvi u peripartalnom periodu i tokom pune laktacije
VL  - 32
IS  - 1
SP  - 9
EP  - 14
DO  - 10.2298/BAH1601009K
ER  - 

@article{
author = "Krsmanović, Milorad and Đoković, Radojica and Cincović, Marko and Ostojić-Andrić, Dušica and Bojkovski, Jovan",
year = "2016",
abstract = "This study examined the activities of aspartate-aminotransferase (AST), gamma-glutamyl-transferase (GGT) and lactate-dehydrogenase (LDH) in the blood serum of 45 dairy Simmental cows divided into three groups according to production period. The first group (n=15) consisted of late pregnant dairy cows, the second group (n=15) cows in the early lactation, and the third group (n=15) cow in mid lactation. The significant higher activity (P lt 0.05) of AST, GGT and LDH were determined in the early lactation period than in dry period and during full lactation. Research results showed possibility of mild degree of hepatic lesions, probably due to fat infiltration in early lactation cows. Serum AST enzyme activities were significant correlated (P lt 0.05) with GGT and LDH activities and may be most sensitive indicator., U ovom radu je određivana aktivnost aspartat-aminotransferaze (AST), gama-glutamat-transferaze (GGT) i laktat dehidrogenaze (LDH) u krvnom serumu kod 45 simentalskih mlečnih krava, podeljenih u tri grupe u zavisnosti od produktivnog perioda. Prvu grupu (n=15) su činile visoko gravidne krave, drugu grupu (n=15) krave u ranoj laktaciji, a treću grupu (n=15) krave tokom pune laktacije. Statistički značajno veće aktivnosti AST (P lt 0.05),GGT (P lt 0.05) i LDH (P lt 0.05) u krvnom serumu su utvrđene kod krava u ranoj laktaciji u odnosu na aktivnosti ovih enzima u serumu kod zasušenih krava i krava u punoj laktaciji. Dobijeni rezultati ukazuju na mogućnost blagog stepena oštećenja ćelija jetre, odnosno masnu infiltraciju hepatocita kod krava na početku laktacije. Serumske aktivnosti AST su bile u značajnoj korelaciji (P lt 0.05) sa aktivnostima GGT i LDH u krvnom serumu pa se AST može smatrati pouzdanim indikatorom u tvrđivanju funkcionalnog stanja jetre kod mlečnih krava tokom peripartalnog perioda i pune laktacije.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations, Određivanje aktivnosti specifičnih enzima krvi u peripartalnom periodu i tokom pune laktacije",
volume = "32",
number = "1",
pages = "9-14",
doi = "10.2298/BAH1601009K"
}

Krsmanović, M., Đoković, R., Cincović, M., Ostojić-Andrić, D.,& Bojkovski, J.. (2016). Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 9-14.
https://doi.org/10.2298/BAH1601009K

Krsmanović M, Đoković R, Cincović M, Ostojić-Andrić D, Bojkovski J. Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations. in Biotechnology in Animal Husbandry. 2016;32(1):9-14.
doi:10.2298/BAH1601009K .

Krsmanović, Milorad, Đoković, Radojica, Cincović, Marko, Ostojić-Andrić, Dušica, Bojkovski, Jovan, "Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):9-14,
https://doi.org/10.2298/BAH1601009K . .

TY  - JOUR
AU  - Cvetojević, Đorđe
AU  - Savić, Božidar
AU  - Stanojević, Slobodan
AU  - Kureljušić, Branislav
AU  - Jezdimirović, Nemanja
AU  - Bojković-Kovačević, Slavica
AU  - Pavlović, Miloš
AU  - Katić, Marko
AU  - Jakić-Dimić, Dobrila
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1298
AB  - Abortions represents significant problem in modern cattle husbandry because they directly and indirectly affects profit from production. Abortions in cattle can be caused by infectious (viruses, bacteria, protozoa, fungi) or non-infectious agents. Infectious agents can have beside their abortive effect other organ pathology which additionally compromise health of animal and also production. Also, some causative agents are zoonotic. This paper represents results from diagnostic examination of samples from cows which aborted on PKB farms during 2014. By polymerase chain reaction (PCR), we investigated presence of genom of BVD virus, IBR virus, Schmallenberg virus, Neospora caninum and Coxiella burnetii. Samples (fetus, placenta, swabs) from 137 cows which aborted were examined and 46 cows (33,5%) were positive on some of causative agents. Presence of one of pathogens was detected in 43 cows (31,4%o), while samples from 3 cows (2,2%o) were positive for two pathogens simultaneously. According to this results, the most common agents were Neospora caninum and BVD virus which were detected in 23 (16,8%) and 19 (13,8%) cows, respectively. Coxiella burnetii was proven in 6 cows (4,4%), IBR virus in one, while presence of Schmallenberg virus was not detected in any cow. Current results suggests that contagious abortive agents are present significantly on PKB corporation farms. Tight cooperation between veterinary service and diagnostic laboratory is necessary for their control and detection.
AB  - Abortusi predstavljaju značajan problem u savremenom uzgoju goveda jer direktno i indirektno utiču na ekonomsku isplativost proizvodnje. Pobačaji goveda mogu biti izazvani infektivnim (virusi, bakterije, protozoe, gljivice) ili neinfektivnim faktorima. Infektivni agensi kao uzročnici pobačaja mogu pored abortogenog efekta imati i drugu organopatologiju čime dodatno ugrožavaju zdravstveno stanje životinja, a time i proizvodnju. Takođe, pojedini uzročnici imaju i zoonotski potencijal. U ovom radu prikazani su rezultati dijagnostičkih ispitivanja uzoraka poreklom od krava koje su abortirale sa gazdinstava PKB-a tokom 2014. godine. Metodom reakcije lančane polimeraze (PCR) ispitivano je prisustvo genoma BVD virusa, IBR virusa, Šmalenberg virusa, Neospora caninum i Coxiella burnetii. Pregledani su uzorci (fetus, placenta ili brisevi) od 137 krava koje su abortirale pri čemu je 46 krava (33,5%) bilo pozitivno na neki od ispitivanih agenasa. Prisustvo jednog od patogena je detektovano kod 43 krave (31,4%o), dok su uzorci od 3 krave (2,2%o) bili istovremeno pozitivni na 2 uzročnika. Prema ovim rezultatima, najzastupljeniji uzročnici su Neospora caninum i BVD virus koji su dokazani kod 23 (16,8%), odnosno 19 (13,8%) krava. Coxiella burnetii je dokazana kod 6 krava (4,4%), IBR virus kod jedne, dok prisustvo genoma Šmalenberg virusa nije ustanovljeno ni kod jedne krave. Trenutni rezultati ukazuju da su kontagiozni uzročnici abortusa prisutni u značajnoj meri na gazdinstvima PKB korporacije. Za njihovu kontrolu i otkrivanje neophodna je bliska saradnja veterinarske službe i dijagnostičke laboratorije.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014
T1  - Pobačaji goveda uzrokovani kontagioznim infektivnim agensima na gazdinstvima PKB korporacije tokom 2014. godine
VL  - 21
IS  - 3-4
SP  - 1
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1298
ER  - 

@article{
author = "Cvetojević, Đorđe and Savić, Božidar and Stanojević, Slobodan and Kureljušić, Branislav and Jezdimirović, Nemanja and Bojković-Kovačević, Slavica and Pavlović, Miloš and Katić, Marko and Jakić-Dimić, Dobrila",
year = "2015",
abstract = "Abortions represents significant problem in modern cattle husbandry because they directly and indirectly affects profit from production. Abortions in cattle can be caused by infectious (viruses, bacteria, protozoa, fungi) or non-infectious agents. Infectious agents can have beside their abortive effect other organ pathology which additionally compromise health of animal and also production. Also, some causative agents are zoonotic. This paper represents results from diagnostic examination of samples from cows which aborted on PKB farms during 2014. By polymerase chain reaction (PCR), we investigated presence of genom of BVD virus, IBR virus, Schmallenberg virus, Neospora caninum and Coxiella burnetii. Samples (fetus, placenta, swabs) from 137 cows which aborted were examined and 46 cows (33,5%) were positive on some of causative agents. Presence of one of pathogens was detected in 43 cows (31,4%o), while samples from 3 cows (2,2%o) were positive for two pathogens simultaneously. According to this results, the most common agents were Neospora caninum and BVD virus which were detected in 23 (16,8%) and 19 (13,8%) cows, respectively. Coxiella burnetii was proven in 6 cows (4,4%), IBR virus in one, while presence of Schmallenberg virus was not detected in any cow. Current results suggests that contagious abortive agents are present significantly on PKB corporation farms. Tight cooperation between veterinary service and diagnostic laboratory is necessary for their control and detection., Abortusi predstavljaju značajan problem u savremenom uzgoju goveda jer direktno i indirektno utiču na ekonomsku isplativost proizvodnje. Pobačaji goveda mogu biti izazvani infektivnim (virusi, bakterije, protozoe, gljivice) ili neinfektivnim faktorima. Infektivni agensi kao uzročnici pobačaja mogu pored abortogenog efekta imati i drugu organopatologiju čime dodatno ugrožavaju zdravstveno stanje životinja, a time i proizvodnju. Takođe, pojedini uzročnici imaju i zoonotski potencijal. U ovom radu prikazani su rezultati dijagnostičkih ispitivanja uzoraka poreklom od krava koje su abortirale sa gazdinstava PKB-a tokom 2014. godine. Metodom reakcije lančane polimeraze (PCR) ispitivano je prisustvo genoma BVD virusa, IBR virusa, Šmalenberg virusa, Neospora caninum i Coxiella burnetii. Pregledani su uzorci (fetus, placenta ili brisevi) od 137 krava koje su abortirale pri čemu je 46 krava (33,5%) bilo pozitivno na neki od ispitivanih agenasa. Prisustvo jednog od patogena je detektovano kod 43 krave (31,4%o), dok su uzorci od 3 krave (2,2%o) bili istovremeno pozitivni na 2 uzročnika. Prema ovim rezultatima, najzastupljeniji uzročnici su Neospora caninum i BVD virus koji su dokazani kod 23 (16,8%), odnosno 19 (13,8%) krava. Coxiella burnetii je dokazana kod 6 krava (4,4%), IBR virus kod jedne, dok prisustvo genoma Šmalenberg virusa nije ustanovljeno ni kod jedne krave. Trenutni rezultati ukazuju da su kontagiozni uzročnici abortusa prisutni u značajnoj meri na gazdinstvima PKB korporacije. Za njihovu kontrolu i otkrivanje neophodna je bliska saradnja veterinarske službe i dijagnostičke laboratorije.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014, Pobačaji goveda uzrokovani kontagioznim infektivnim agensima na gazdinstvima PKB korporacije tokom 2014. godine",
volume = "21",
number = "3-4",
pages = "1-6",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1298"
}

Cvetojević, Đ., Savić, B., Stanojević, S., Kureljušić, B., Jezdimirović, N., Bojković-Kovačević, S., Pavlović, M., Katić, M.,& Jakić-Dimić, D.. (2015). Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 21(3-4), 1-6.
https://hdl.handle.net/21.15107/rcub_veterinar_1298

Cvetojević Đ, Savić B, Stanojević S, Kureljušić B, Jezdimirović N, Bojković-Kovačević S, Pavlović M, Katić M, Jakić-Dimić D. Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2015;21(3-4):1-6.
https://hdl.handle.net/21.15107/rcub_veterinar_1298 .

Cvetojević, Đorđe, Savić, Božidar, Stanojević, Slobodan, Kureljušić, Branislav, Jezdimirović, Nemanja, Bojković-Kovačević, Slavica, Pavlović, Miloš, Katić, Marko, Jakić-Dimić, Dobrila, "Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014" in Zbornik naučnih radova Instituta PKB Agroekonomik, 21, no. 3-4 (2015):1-6,
https://hdl.handle.net/21.15107/rcub_veterinar_1298 .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Stanković, Branislav
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1297
AB  - Intensive cattle farming covers a wide range of technological processes that should enable continued production and optimal use of production capacity. In such circumstances newborn calves have to adapt to different environmental factors, including diet. The technology of growing calves diet was initially based exclusively on a diet of colostrum and then milk. After that, the food introduced other feed (hay, feed mixture), but after three months the milk completely excluded from the diet. For high - dairy cows metabolic disorders such as ketosis, fatty liver syndrome, puerperal paresis, rumen acidosis, laminitis, have been caused by many factors, such as non adequate nutrition nutrition, poor housing conditions and care. Prophylactic treatment of metabolic disorders is done with adequate nutrition that needs to be matched by production meal and product category with health control cows.
AB  - Intenzivan uzgoj goveda obuhvata niz tehnoloških postupaka koji treba da omoguće kontinuiranu proizvodnju i optimalno korišćenje proizvodnih kapaciteta. U takvim uslovima novorođena telad moraju da se prilagode na različite činioce okoline, uključujući i način ishrane. U tehnologiji uzgoja teladi ishrana je u početku bazirana isključivo na ishrani kolostrumom, a potom mlekom. Nakon toga se u hranu uvode druga hraniva (seno, krmna smeša), da bi se posle tri meseca mleko potpuno isključilo iz ishrane. Kod visoko mlečnih kava metabolički poremećaji kao što su ketoza, sindrom masne jetre, puerperalna pareza, acidoza buraga, laminitis, su posledica uticaja mnogih činilaca, kao što su neizbalnsirana ishrana, loši usovi držanja i nege. Profilaksa metaboličkih poremećaja krava se obavlja ishranom koja treba da bude usklađena prema proizvodnom obroku i proizvodnoj kategoriji uz stalnu kontrolu zdravlja.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Health calves and cows holstein friesian race in puerperium (review research)
T1  - Zdravstveno stanje teladi i krava holštajn frizijske rase u puerperijumu (pregled istraživanja)
VL  - 21
IS  - 3-4
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3965
ER  - 

@article{
author = "Bojkovski, Jovan and Stanković, Branislav",
year = "2015",
abstract = "Intensive cattle farming covers a wide range of technological processes that should enable continued production and optimal use of production capacity. In such circumstances newborn calves have to adapt to different environmental factors, including diet. The technology of growing calves diet was initially based exclusively on a diet of colostrum and then milk. After that, the food introduced other feed (hay, feed mixture), but after three months the milk completely excluded from the diet. For high - dairy cows metabolic disorders such as ketosis, fatty liver syndrome, puerperal paresis, rumen acidosis, laminitis, have been caused by many factors, such as non adequate nutrition nutrition, poor housing conditions and care. Prophylactic treatment of metabolic disorders is done with adequate nutrition that needs to be matched by production meal and product category with health control cows., Intenzivan uzgoj goveda obuhvata niz tehnoloških postupaka koji treba da omoguće kontinuiranu proizvodnju i optimalno korišćenje proizvodnih kapaciteta. U takvim uslovima novorođena telad moraju da se prilagode na različite činioce okoline, uključujući i način ishrane. U tehnologiji uzgoja teladi ishrana je u početku bazirana isključivo na ishrani kolostrumom, a potom mlekom. Nakon toga se u hranu uvode druga hraniva (seno, krmna smeša), da bi se posle tri meseca mleko potpuno isključilo iz ishrane. Kod visoko mlečnih kava metabolički poremećaji kao što su ketoza, sindrom masne jetre, puerperalna pareza, acidoza buraga, laminitis, su posledica uticaja mnogih činilaca, kao što su neizbalnsirana ishrana, loši usovi držanja i nege. Profilaksa metaboličkih poremećaja krava se obavlja ishranom koja treba da bude usklađena prema proizvodnom obroku i proizvodnoj kategoriji uz stalnu kontrolu zdravlja.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Health calves and cows holstein friesian race in puerperium (review research), Zdravstveno stanje teladi i krava holštajn frizijske rase u puerperijumu (pregled istraživanja)",
volume = "21",
number = "3-4",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3965"
}

Bojkovski, J.,& Stanković, B.. (2015). Health calves and cows holstein friesian race in puerperium (review research). in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 21(3-4), 13-22.
https://hdl.handle.net/21.15107/rcub_agrospace_3965

Bojkovski J, Stanković B. Health calves and cows holstein friesian race in puerperium (review research). in Zbornik naučnih radova Instituta PKB Agroekonomik. 2015;21(3-4):13-22.
https://hdl.handle.net/21.15107/rcub_agrospace_3965 .

Bojkovski, Jovan, Stanković, Branislav, "Health calves and cows holstein friesian race in puerperium (review research)" in Zbornik naučnih radova Instituta PKB Agroekonomik, 21, no. 3-4 (2015):13-22,
https://hdl.handle.net/21.15107/rcub_agrospace_3965 .

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Kureljušić, Jasna
AU  - Cvetojević, Đ.
AU  - Maslić-Strižak, Danka
AU  - Radanović, O.
AU  - Jezdimirović, Milanka
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1921
AB  - Ispitivanja su imala za cilj da se utvrdi uticaj Clostridium perfringens tip A na razvoj patomorfološkog supstrata, njegov intenzitet i distribuciju kod teških roditelja uzrasta petnaest nedelja. Ispitivanje je obavljeno na leševima 8 koka i 7 petlova teških roditelja provenijencije COBB 500. Posle izvršene obdukcije, za histopatološko ispitivanje uzeti su uzorci promenjenih delova jejunuma i jetre, a za bakteriološko i molekularno ispitivanje crevni sadržaj jejunuma.  Kod svih leševa u otvorenoj pleuroperitonealnoj šupljini, već in situ, uočava se promenjeni deo jejunuma. On je bio izrazito dilatiran celom dužinom, a njegov zid plavičasto-sive boje sa diseminiranim subseroznim punktiformnim krvnim ekstravazatima. Njegovim otvaranjem iz lumena je izlazio polutečni sadržaj u kojem plivaju krvni koagulumi i krpičasti delovi nekrotične mukoze. Mikroskopskim pregledom isečaka tkiva tankih creva bojenih HE metodom zapažena je difuzna nekroza crevnih resica. One su destruisane i zamenjene eozinofilnom bestrukturnom masom. Pored toga, uočava se edem submukoze, kongestija kapilara i krvni ekstravazati u mukozi, kao i infiltracija neutrofilnih granulocita u lamini propriji. Navedene mikroskopske alteracije oslikavaju hemoragično-nekrotični enteritis. Mikroskopskim pregledom isečka tankih creva bojenih Brown & Brenn metodom ustanovljene su kolonije bakterija u distalnim delovima submukoze.  Bakteriološkim ispitivanjem u anaerobnim uslovima, izolovana kultura je identifikovana kao Clostridium perfringens. Primenom multiplex PCR dobijeni izolat je genotipiziran kao Clostridium perfringens tip A. Na osnovu patomorfoloških, bakterioloških i molekularnih ispitivanja može da se zaključi da infekcija teških roditelja Clostridium perfringens tipa A protiče sa slikom difuznog hemoragično-nekrotičnog jejunitisa, da uzročnik duboko penetrira u tkivo jejunuma i da su pšenica i pšenične mekinje činili favorizujući faktor za proliferaciju etiološkog agensa.
AB  - The aim of the investigation was to determine the influence of Clostridium perfringens type A on the development of pathomorphological substrate, its intensity and distribution in fifteen weeks old heavy breeds broilers. The investigation was carried out on corpses of 8 hens and 7 roosters of heavy breeds of provenance COBB 500. After the completion of the autopsy, samples of altered parts of jejunum and liver were taken for histopathological examination, and jejunum intestinal contents for bacteriological examination. In all the corpses, in open pleuroperitoneal cavity, even in situ, an altered part of jejunum can be noticed. It was extremely dilated the entire length, and its wall was bluish-gray with disseminated subserous punctiform blood extravasates. When opened, semi-liquid content with blood coagulums and patches of necrotic mucosa went out of it. By microscopic examination of small intestine tissue cuttings, colored by HE method, there was observed a diffuse necrosis of intestinal villi. They were desroyed and replaced by eosinophilic structureless mass. Furthermore, there could be noticed submucose oedema, capillary congestion and blood extravasates in mucosa, as well as infiltration of neutrophilic granulocytes in lamina propria. These microscopic alterations reflect hemorrhagic necrotic enteritis. By microscopic examining of small intestine cuttings colored according to Brown & Brenn method, colonies of bacteria in distal parts of the submucosa were found out.  Using bacteriological tests in anaerobic conditions, there was isolated a culture identified as Clostridium perfringens. After applying of multiplex PCR, the obtained isolate was genotyped as Clostridium perfringens type A.  On the basis of pathomorphological, bacteriological and molecular examinations, it can be concluded that the infection of heavy breeds with Clostridium perfringens type A is manifested by appearance of haemorrhagic-necrotic jejunitis, that the causer penetrates deeply into jejunum tissue and that wheat and wheat bran were a favoring factor for proliferation of the etiological agent.
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Haemorrhagic-necrotic enteritis in heavy breeds broilers
T1  - Hemoragično-nekrotični enteritis kod roditelja brojlera
VL  - 68
IS  - 3-4
SP  - 265
EP  - 273
DO  - 10.2298/VETGL1404265J
ER  - 

@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Ivetić, Vojin and Kureljušić, Jasna and Cvetojević, Đ. and Maslić-Strižak, Danka and Radanović, O. and Jezdimirović, Milanka",
year = "2014",
abstract = "Ispitivanja su imala za cilj da se utvrdi uticaj Clostridium perfringens tip A na razvoj patomorfološkog supstrata, njegov intenzitet i distribuciju kod teških roditelja uzrasta petnaest nedelja. Ispitivanje je obavljeno na leševima 8 koka i 7 petlova teških roditelja provenijencije COBB 500. Posle izvršene obdukcije, za histopatološko ispitivanje uzeti su uzorci promenjenih delova jejunuma i jetre, a za bakteriološko i molekularno ispitivanje crevni sadržaj jejunuma.  Kod svih leševa u otvorenoj pleuroperitonealnoj šupljini, već in situ, uočava se promenjeni deo jejunuma. On je bio izrazito dilatiran celom dužinom, a njegov zid plavičasto-sive boje sa diseminiranim subseroznim punktiformnim krvnim ekstravazatima. Njegovim otvaranjem iz lumena je izlazio polutečni sadržaj u kojem plivaju krvni koagulumi i krpičasti delovi nekrotične mukoze. Mikroskopskim pregledom isečaka tkiva tankih creva bojenih HE metodom zapažena je difuzna nekroza crevnih resica. One su destruisane i zamenjene eozinofilnom bestrukturnom masom. Pored toga, uočava se edem submukoze, kongestija kapilara i krvni ekstravazati u mukozi, kao i infiltracija neutrofilnih granulocita u lamini propriji. Navedene mikroskopske alteracije oslikavaju hemoragično-nekrotični enteritis. Mikroskopskim pregledom isečka tankih creva bojenih Brown & Brenn metodom ustanovljene su kolonije bakterija u distalnim delovima submukoze.  Bakteriološkim ispitivanjem u anaerobnim uslovima, izolovana kultura je identifikovana kao Clostridium perfringens. Primenom multiplex PCR dobijeni izolat je genotipiziran kao Clostridium perfringens tip A. Na osnovu patomorfoloških, bakterioloških i molekularnih ispitivanja može da se zaključi da infekcija teških roditelja Clostridium perfringens tipa A protiče sa slikom difuznog hemoragično-nekrotičnog jejunitisa, da uzročnik duboko penetrira u tkivo jejunuma i da su pšenica i pšenične mekinje činili favorizujući faktor za proliferaciju etiološkog agensa., The aim of the investigation was to determine the influence of Clostridium perfringens type A on the development of pathomorphological substrate, its intensity and distribution in fifteen weeks old heavy breeds broilers. The investigation was carried out on corpses of 8 hens and 7 roosters of heavy breeds of provenance COBB 500. After the completion of the autopsy, samples of altered parts of jejunum and liver were taken for histopathological examination, and jejunum intestinal contents for bacteriological examination. In all the corpses, in open pleuroperitoneal cavity, even in situ, an altered part of jejunum can be noticed. It was extremely dilated the entire length, and its wall was bluish-gray with disseminated subserous punctiform blood extravasates. When opened, semi-liquid content with blood coagulums and patches of necrotic mucosa went out of it. By microscopic examination of small intestine tissue cuttings, colored by HE method, there was observed a diffuse necrosis of intestinal villi. They were desroyed and replaced by eosinophilic structureless mass. Furthermore, there could be noticed submucose oedema, capillary congestion and blood extravasates in mucosa, as well as infiltration of neutrophilic granulocytes in lamina propria. These microscopic alterations reflect hemorrhagic necrotic enteritis. By microscopic examining of small intestine cuttings colored according to Brown & Brenn method, colonies of bacteria in distal parts of the submucosa were found out.  Using bacteriological tests in anaerobic conditions, there was isolated a culture identified as Clostridium perfringens. After applying of multiplex PCR, the obtained isolate was genotyped as Clostridium perfringens type A.  On the basis of pathomorphological, bacteriological and molecular examinations, it can be concluded that the infection of heavy breeds with Clostridium perfringens type A is manifested by appearance of haemorrhagic-necrotic jejunitis, that the causer penetrates deeply into jejunum tissue and that wheat and wheat bran were a favoring factor for proliferation of the etiological agent.",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Haemorrhagic-necrotic enteritis in heavy breeds broilers, Hemoragično-nekrotični enteritis kod roditelja brojlera",
volume = "68",
number = "3-4",
pages = "265-273",
doi = "10.2298/VETGL1404265J"
}

Jezdimirović, N., Kureljušić, B., Ivetić, V., Kureljušić, J., Cvetojević, Đ., Maslić-Strižak, D., Radanović, O.,& Jezdimirović, M.. (2014). Haemorrhagic-necrotic enteritis in heavy breeds broilers. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 68(3-4), 265-273.
https://doi.org/10.2298/VETGL1404265J

Jezdimirović N, Kureljušić B, Ivetić V, Kureljušić J, Cvetojević Đ, Maslić-Strižak D, Radanović O, Jezdimirović M. Haemorrhagic-necrotic enteritis in heavy breeds broilers. in Veterinarski Glasnik. 2014;68(3-4):265-273.
doi:10.2298/VETGL1404265J .

Jezdimirović, Nemanja, Kureljušić, Branislav, Ivetić, Vojin, Kureljušić, Jasna, Cvetojević, Đ., Maslić-Strižak, Danka, Radanović, O., Jezdimirović, Milanka, "Haemorrhagic-necrotic enteritis in heavy breeds broilers" in Veterinarski Glasnik, 68, no. 3-4 (2014):265-273,
https://doi.org/10.2298/VETGL1404265J . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Šamanc, Horea
AU  - Vujanac, Ivan
AU  - Ivetić, Vojin
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2490
AB  - The objective of the study was to estimate herd-basis energy status (ES) using body condition scoring (BCS) and shortened metabolic profiles in multiparous clinically healthy dairy cows and to evaluate if such profiles can be informative in herd investigation to indicate on metabolic herd problems. A total of 180 Holstein cows from commercial herd were clinically examined and assigned to one of 3 equal groups: dry cows (15 days before expected calving), puerperal (10 days after calving) and cows at day 60 of lactation. Blood were sampled and analyzed for total protein, albumin, urea nitrogen (UN), β-hydroxybutyrate (BHBA), glucose and total bilirubin. Liver samples by percutaneous biopsies were obtained at puerperal period and tested for lipid content. The cows had a mean BCS of 3.92±0.03 at dry period, 3.08 ± 0.03 at puerperal period and 2.82 ± 0.02 at day 60 of lactation. Average total protein, albumin and glucose concentrations were within the physiological range, whereas UN was higher than physiologically accepted, at all examined periods. BHBA levels increased after calving and 61.66% of puerperal cows had BHBA levels above 1 mmol/L. Average total bilirubin concentration after calving was higher than recommended. Concentrations of UN, BHBA and total bilirubin were positively correlated, whereas concentrations of total protein and glucose were negatively correlated with fatty
liver degree. Albumin concentration was not correlated with fatty liver degree. In conclusion, although clinically healthy the examined cows were not in adequate ES during the transition period. Consequently, 55% of the cows had moderate to severe fatty liver. A sufficient number of highly significant correlations suggest that employed metabolic profiles may offer an indirect means of estimation energy status on a herd basis.
PB  - Academic Journals
T2  - African Journal of Agricultural Research
T1  - Estimation of herd-basis energy status in clinically healthy Holstein cows: Practical implications of body condition scoring and shortened metabolic profiles
VL  - 7
SP  - 418
EP  - 425
DO  - 10.5897/AJAR11.1432
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Šamanc, Horea and Vujanac, Ivan and Ivetić, Vojin and Savić, Božidar and Kureljušić, Branislav",
year = "2012",
abstract = "The objective of the study was to estimate herd-basis energy status (ES) using body condition scoring (BCS) and shortened metabolic profiles in multiparous clinically healthy dairy cows and to evaluate if such profiles can be informative in herd investigation to indicate on metabolic herd problems. A total of 180 Holstein cows from commercial herd were clinically examined and assigned to one of 3 equal groups: dry cows (15 days before expected calving), puerperal (10 days after calving) and cows at day 60 of lactation. Blood were sampled and analyzed for total protein, albumin, urea nitrogen (UN), β-hydroxybutyrate (BHBA), glucose and total bilirubin. Liver samples by percutaneous biopsies were obtained at puerperal period and tested for lipid content. The cows had a mean BCS of 3.92±0.03 at dry period, 3.08 ± 0.03 at puerperal period and 2.82 ± 0.02 at day 60 of lactation. Average total protein, albumin and glucose concentrations were within the physiological range, whereas UN was higher than physiologically accepted, at all examined periods. BHBA levels increased after calving and 61.66% of puerperal cows had BHBA levels above 1 mmol/L. Average total bilirubin concentration after calving was higher than recommended. Concentrations of UN, BHBA and total bilirubin were positively correlated, whereas concentrations of total protein and glucose were negatively correlated with fatty
liver degree. Albumin concentration was not correlated with fatty liver degree. In conclusion, although clinically healthy the examined cows were not in adequate ES during the transition period. Consequently, 55% of the cows had moderate to severe fatty liver. A sufficient number of highly significant correlations suggest that employed metabolic profiles may offer an indirect means of estimation energy status on a herd basis.",
publisher = "Academic Journals",
journal = "African Journal of Agricultural Research",
title = "Estimation of herd-basis energy status in clinically healthy Holstein cows: Practical implications of body condition scoring and shortened metabolic profiles",
volume = "7",
pages = "418-425",
doi = "10.5897/AJAR11.1432"
}

Prodanović, R., Kirovski, D., Šamanc, H., Vujanac, I., Ivetić, V., Savić, B.,& Kureljušić, B.. (2012). Estimation of herd-basis energy status in clinically healthy Holstein cows: Practical implications of body condition scoring and shortened metabolic profiles. in African Journal of Agricultural Research
Academic Journals., 7, 418-425.
https://doi.org/10.5897/AJAR11.1432

Prodanović R, Kirovski D, Šamanc H, Vujanac I, Ivetić V, Savić B, Kureljušić B. Estimation of herd-basis energy status in clinically healthy Holstein cows: Practical implications of body condition scoring and shortened metabolic profiles. in African Journal of Agricultural Research. 2012;7:418-425.
doi:10.5897/AJAR11.1432 .

Prodanović, Radiša, Kirovski, Danijela, Šamanc, Horea, Vujanac, Ivan, Ivetić, Vojin, Savić, Božidar, Kureljušić, Branislav, "Estimation of herd-basis energy status in clinically healthy Holstein cows: Practical implications of body condition scoring and shortened metabolic profiles" in African Journal of Agricultural Research, 7 (2012):418-425,
https://doi.org/10.5897/AJAR11.1432 . .

TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Jakić-Dimić, Dobrila
AU  - Bojkovski, Jovan
AU  - Prodanović, Radiša
AU  - Kureljušić, Branislav
AU  - Potkonjak, Aleksandar
AU  - Savić, Borivoje
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/889
AB  - Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.
PB  - Springer Wien, Wien
T2  - Archives of Virology
T1  - Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia
VL  - 157
IS  - 1
SP  - 21
EP  - 28
DO  - 10.1007/s00705-011-1130-9
ER  - 

@article{
author = "Savić, Božidar and Milićević, Vesna and Jakić-Dimić, Dobrila and Bojkovski, Jovan and Prodanović, Radiša and Kureljušić, Branislav and Potkonjak, Aleksandar and Savić, Borivoje",
year = "2012",
abstract = "Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.",
publisher = "Springer Wien, Wien",
journal = "Archives of Virology",
title = "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia",
volume = "157",
number = "1",
pages = "21-28",
doi = "10.1007/s00705-011-1130-9"
}

Savić, B., Milićević, V., Jakić-Dimić, D., Bojkovski, J., Prodanović, R., Kureljušić, B., Potkonjak, A.,& Savić, B.. (2012). Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology
Springer Wien, Wien., 157(1), 21-28.
https://doi.org/10.1007/s00705-011-1130-9

Savić B, Milićević V, Jakić-Dimić D, Bojkovski J, Prodanović R, Kureljušić B, Potkonjak A, Savić B. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. in Archives of Virology. 2012;157(1):21-28.
doi:10.1007/s00705-011-1130-9 .

Savić, Božidar, Milićević, Vesna, Jakić-Dimić, Dobrila, Bojkovski, Jovan, Prodanović, Radiša, Kureljušić, Branislav, Potkonjak, Aleksandar, Savić, Borivoje, "Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia" in Archives of Virology, 157, no. 1 (2012):21-28,
https://doi.org/10.1007/s00705-011-1130-9 . .

TY  - JOUR
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Ivetić, Vojin
AU  - Radanović, Oliver
AU  - Bojkovski, Jovan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/887
AB  - Four 9-month-old Simmental male calves were presented with a history of sudden death. The necropsy and microscopic findings allowed a diagnosis of enteritis and severe intraluminal hemorrhage with blood clots in the jejunum, suggestive of jejunal hemorrhage syndrome.
PB  - Canadian Vet Med Assoc, Ottawa
T2  - Canadian Veterinary Journal-Revue Veterinaire Canadienne
T1  - Enteritis associated with Clostridium perfringens type A in 9-month-old calves
VL  - 53
IS  - 2
SP  - 174
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_887
ER  - 

@article{
author = "Savić, Božidar and Prodanović, Radiša and Ivetić, Vojin and Radanović, Oliver and Bojkovski, Jovan",
year = "2012",
abstract = "Four 9-month-old Simmental male calves were presented with a history of sudden death. The necropsy and microscopic findings allowed a diagnosis of enteritis and severe intraluminal hemorrhage with blood clots in the jejunum, suggestive of jejunal hemorrhage syndrome.",
publisher = "Canadian Vet Med Assoc, Ottawa",
journal = "Canadian Veterinary Journal-Revue Veterinaire Canadienne",
title = "Enteritis associated with Clostridium perfringens type A in 9-month-old calves",
volume = "53",
number = "2",
pages = "174-176",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_887"
}

Savić, B., Prodanović, R., Ivetić, V., Radanović, O.,& Bojkovski, J.. (2012). Enteritis associated with Clostridium perfringens type A in 9-month-old calves. in Canadian Veterinary Journal-Revue Veterinaire Canadienne
Canadian Vet Med Assoc, Ottawa., 53(2), 174-176.
https://hdl.handle.net/21.15107/rcub_veterinar_887

Savić B, Prodanović R, Ivetić V, Radanović O, Bojkovski J. Enteritis associated with Clostridium perfringens type A in 9-month-old calves. in Canadian Veterinary Journal-Revue Veterinaire Canadienne. 2012;53(2):174-176.
https://hdl.handle.net/21.15107/rcub_veterinar_887 .

Savić, Božidar, Prodanović, Radiša, Ivetić, Vojin, Radanović, Oliver, Bojkovski, Jovan, "Enteritis associated with Clostridium perfringens type A in 9-month-old calves" in Canadian Veterinary Journal-Revue Veterinaire Canadienne, 53, no. 2 (2012):174-176,
https://hdl.handle.net/21.15107/rcub_veterinar_887 .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Pavlović, Ivan
AU  - Relić, Renata
AU  - Bugarski, Dejan
AU  - Savić, Božidar
AU  - Panousis, Nikolaos
AU  - Giadinis, Nektarios
AU  - Stanković, Branislav
AU  - Petrujkić, Tihomir
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/929
AB  - Intensive cattle breeding involves a whole series of technological processes, which should allow continued production and optimal use of production capacities. In those conditions newborn calves must adapt to different environmental factors, including diet and housing conditions. Diseases of digestive and respiratory organs are the most common health problems in calves during first months of their life, and they also threat level of their welfare. In this paper the most common health problems of calves in intensive production are discussed.
AB  - Intenzivan uzgoj goveda obuhvata niz tehnoloških postupaka koji treba da omoguće kontinuiranu proizvodnju i optimalno korišćenje proizvodnih kapaciteta. U takvim uslovima novorođena telad mora da se prilagodi različitim činiocima iz okoline, uključujući način ishrane i uslove smeštaja. Zdravstveno stanje i dobrobit teladi u prvim mesecima života najčešće ugrožavaju oboljenja organa za varenje i disanje. U ovom radu razmatrani su najčešći zdravstveni problemi teladi u intenzivnoj proizvodnji.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Health problems and welfare of calves in intensive production
T1  - Zdravstveni problemi i dobrobit teladi u intenzivnoj proizvodnji
VL  - 18
IS  - 3-4
SP  - 85
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3079
ER  - 

@article{
author = "Bojkovski, Jovan and Pavlović, Ivan and Relić, Renata and Bugarski, Dejan and Savić, Božidar and Panousis, Nikolaos and Giadinis, Nektarios and Stanković, Branislav and Petrujkić, Tihomir",
year = "2012",
abstract = "Intensive cattle breeding involves a whole series of technological processes, which should allow continued production and optimal use of production capacities. In those conditions newborn calves must adapt to different environmental factors, including diet and housing conditions. Diseases of digestive and respiratory organs are the most common health problems in calves during first months of their life, and they also threat level of their welfare. In this paper the most common health problems of calves in intensive production are discussed., Intenzivan uzgoj goveda obuhvata niz tehnoloških postupaka koji treba da omoguće kontinuiranu proizvodnju i optimalno korišćenje proizvodnih kapaciteta. U takvim uslovima novorođena telad mora da se prilagodi različitim činiocima iz okoline, uključujući način ishrane i uslove smeštaja. Zdravstveno stanje i dobrobit teladi u prvim mesecima života najčešće ugrožavaju oboljenja organa za varenje i disanje. U ovom radu razmatrani su najčešći zdravstveni problemi teladi u intenzivnoj proizvodnji.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Health problems and welfare of calves in intensive production, Zdravstveni problemi i dobrobit teladi u intenzivnoj proizvodnji",
volume = "18",
number = "3-4",
pages = "85-91",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3079"
}

Bojkovski, J., Pavlović, I., Relić, R., Bugarski, D., Savić, B., Panousis, N., Giadinis, N., Stanković, B.,& Petrujkić, T.. (2012). Health problems and welfare of calves in intensive production. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 18(3-4), 85-91.
https://hdl.handle.net/21.15107/rcub_agrospace_3079

Bojkovski J, Pavlović I, Relić R, Bugarski D, Savić B, Panousis N, Giadinis N, Stanković B, Petrujkić T. Health problems and welfare of calves in intensive production. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2012;18(3-4):85-91.
https://hdl.handle.net/21.15107/rcub_agrospace_3079 .

Bojkovski, Jovan, Pavlović, Ivan, Relić, Renata, Bugarski, Dejan, Savić, Božidar, Panousis, Nikolaos, Giadinis, Nektarios, Stanković, Branislav, Petrujkić, Tihomir, "Health problems and welfare of calves in intensive production" in Zbornik naučnih radova Instituta PKB Agroekonomik, 18, no. 3-4 (2012):85-91,
https://hdl.handle.net/21.15107/rcub_agrospace_3079 .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Ivetić, V.
AU  - Savić, Božidar
AU  - Kureljušić, Branislav
AU  - Stevančević, M.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/906
AB  - The aim of this study was to assess the significance of body condition scoring and metabolic profile test for estimation of energy status of healthy high-yielding dairy cows. Twenty one healthy cows (primiparous and secundiparous) were divided into three groups: dry cows, early puerperal cows and early lactating cows. Cow's energy status was estimated by the analysis of blood samples for beta-hydroxybutirate (BHBA) and glucose. Additionally, urea, total bilirubine and total protein were measured in blood serum samples. According to body condition scores (BCS) results dry cows were overweight (4.03±0.29 points). Immediately after calving, cows lost their weight significantly, since BCS was 2.85±0.46 points at puerperal period and 3.12±0.33 points at day 60 of lactation (p lt 0.001 compared to dry period, respectively). Glucose concentration did not change significantly between dry and puerperal period, while BHBA increased significantly (0.46±0.14 mmol/l at dry period to 1.08±0.21 mmol/l at puerperal period; p lt 0.001). Urea concentration did not change significantly during examined period. Total bilirubin concentration significantly increased from dry (6.31±0.37 mmol/l) to puerperal period (7.63±2.52 mmol/l; p lt 0.001 compared to dry period) and remained high until day 60 of lactation (7.62±0.13 mmol/l; p lt 0.001 compared to dry period). Total protein concentration decreased from dry to puerperal period (69.59±6.14 g/l to 58.87±3.29 g/l; p lt 0.001). According to obtained results it can be concluded that cows were not in adequate energy status during transition period. Our results also indicate that, body condition scoring, BHBA and total bilirubin concentrations can be used as reliable indicators of cow's energy status even when there are not clinically visible health disorders.
AB  - Cilj istraživanja bio je da se ukaže na značaj metaboličkog profila i telesne kondicije u oceni energetskog statusa visoko-mlečnih krava holštajn rase. Za ispitivanje su odabrane tri grupe po sedam krava. Uzorci krvi uzimani su u zasušenju (15 dana pre očekivanog teljenja), ranom puerperijumu (7 dana nakon teljenja) i 60. dana laktacije. U uzorcima sveže krvi određivana je koncentracija glukoze i beta hidroksi-buterne kiseline (BHBA), a u uzorcima krvnog seruma koncentracija ukupnih proteina, ureje i ukupnog bilirubina. Rezultati ocene telesne kondicije pokazuju da su krave u periodu zasušenja preuhranjene (X=4,03±0,29 poena), dok su u puerperijumu (X=2,85±0,46 poena) i 60. dana laktacije (X=3,12±0,33 poena) u značajno slabijoj telesnoj kondiciji (p lt 0,001 u odnosu na zasušenje). Rezultati ispitivanja parametara metaboličkog profila ukazuju da je koncentracija proteina jedino u periodu ranog puerperijuma bila niža od fiziološke vrednosti (X=56,87±3,29 g/l). Koncentracija glukoze nije se statistički značajno menjala tokom ispitivanih perioda, dok je neposredno posle teljenja došlo do značajnog povećanja koncentracije BHBA (p lt 0,001 u odnosu na zasušenje). Uremija u periodu zasušenja (X=7,73±2,34 mmol/l), ranom puerperijumu (X=8,07±3,09 mmol/l) i 60. dana laktacije (X=7,41±2,03 mmol/l) bila je veća od gornje fiziološke granice. Koncentracija ukupnog bilirubina u periodu zasušenja je bila značajno niža (p lt 0,001) u odnosu na oba ispitivana perioda nakon teljenja. Na osnovu dobijenih rezultata može se zaključiti da krave nisu adekvatno pripremljene za nastupajuću laktaciju, zbog čega je kod njih došlo do značajnog odstupanja u telesnoj kondiciji i vrednostima parametara metaboličkog profila. Ova odstupanja ukazuju na nedovoljnu sposobnost životinja da se prilagode u uslovima negativnog bilansa energije na početku laktacije.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows
T1  - Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila
VL  - 28
IS  - 1
SP  - 25
EP  - 32
DO  - 10.2298/BAH1201025P
ER  - 

@article{
author = "Prodanović, Radiša and Sladojević, Željko and Kirovski, Danijela and Vujanac, Ivan and Ivetić, V. and Savić, Božidar and Kureljušić, Branislav and Stevančević, M.",
year = "2012",
abstract = "The aim of this study was to assess the significance of body condition scoring and metabolic profile test for estimation of energy status of healthy high-yielding dairy cows. Twenty one healthy cows (primiparous and secundiparous) were divided into three groups: dry cows, early puerperal cows and early lactating cows. Cow's energy status was estimated by the analysis of blood samples for beta-hydroxybutirate (BHBA) and glucose. Additionally, urea, total bilirubine and total protein were measured in blood serum samples. According to body condition scores (BCS) results dry cows were overweight (4.03±0.29 points). Immediately after calving, cows lost their weight significantly, since BCS was 2.85±0.46 points at puerperal period and 3.12±0.33 points at day 60 of lactation (p lt 0.001 compared to dry period, respectively). Glucose concentration did not change significantly between dry and puerperal period, while BHBA increased significantly (0.46±0.14 mmol/l at dry period to 1.08±0.21 mmol/l at puerperal period; p lt 0.001). Urea concentration did not change significantly during examined period. Total bilirubin concentration significantly increased from dry (6.31±0.37 mmol/l) to puerperal period (7.63±2.52 mmol/l; p lt 0.001 compared to dry period) and remained high until day 60 of lactation (7.62±0.13 mmol/l; p lt 0.001 compared to dry period). Total protein concentration decreased from dry to puerperal period (69.59±6.14 g/l to 58.87±3.29 g/l; p lt 0.001). According to obtained results it can be concluded that cows were not in adequate energy status during transition period. Our results also indicate that, body condition scoring, BHBA and total bilirubin concentrations can be used as reliable indicators of cow's energy status even when there are not clinically visible health disorders., Cilj istraživanja bio je da se ukaže na značaj metaboličkog profila i telesne kondicije u oceni energetskog statusa visoko-mlečnih krava holštajn rase. Za ispitivanje su odabrane tri grupe po sedam krava. Uzorci krvi uzimani su u zasušenju (15 dana pre očekivanog teljenja), ranom puerperijumu (7 dana nakon teljenja) i 60. dana laktacije. U uzorcima sveže krvi određivana je koncentracija glukoze i beta hidroksi-buterne kiseline (BHBA), a u uzorcima krvnog seruma koncentracija ukupnih proteina, ureje i ukupnog bilirubina. Rezultati ocene telesne kondicije pokazuju da su krave u periodu zasušenja preuhranjene (X=4,03±0,29 poena), dok su u puerperijumu (X=2,85±0,46 poena) i 60. dana laktacije (X=3,12±0,33 poena) u značajno slabijoj telesnoj kondiciji (p lt 0,001 u odnosu na zasušenje). Rezultati ispitivanja parametara metaboličkog profila ukazuju da je koncentracija proteina jedino u periodu ranog puerperijuma bila niža od fiziološke vrednosti (X=56,87±3,29 g/l). Koncentracija glukoze nije se statistički značajno menjala tokom ispitivanih perioda, dok je neposredno posle teljenja došlo do značajnog povećanja koncentracije BHBA (p lt 0,001 u odnosu na zasušenje). Uremija u periodu zasušenja (X=7,73±2,34 mmol/l), ranom puerperijumu (X=8,07±3,09 mmol/l) i 60. dana laktacije (X=7,41±2,03 mmol/l) bila je veća od gornje fiziološke granice. Koncentracija ukupnog bilirubina u periodu zasušenja je bila značajno niža (p lt 0,001) u odnosu na oba ispitivana perioda nakon teljenja. Na osnovu dobijenih rezultata može se zaključiti da krave nisu adekvatno pripremljene za nastupajuću laktaciju, zbog čega je kod njih došlo do značajnog odstupanja u telesnoj kondiciji i vrednostima parametara metaboličkog profila. Ova odstupanja ukazuju na nedovoljnu sposobnost životinja da se prilagode u uslovima negativnog bilansa energije na početku laktacije.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows, Procena energetskog statusa krava na osnovu telesne kondicije i parametara metaboličkog profila",
volume = "28",
number = "1",
pages = "25-32",
doi = "10.2298/BAH1201025P"
}

Prodanović, R., Sladojević, Ž., Kirovski, D., Vujanac, I., Ivetić, V., Savić, B., Kureljušić, B.,& Stevančević, M.. (2012). Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(1), 25-32.
https://doi.org/10.2298/BAH1201025P

Prodanović R, Sladojević Ž, Kirovski D, Vujanac I, Ivetić V, Savić B, Kureljušić B, Stevančević M. Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. in Biotechnology in Animal Husbandry. 2012;28(1):25-32.
doi:10.2298/BAH1201025P .

Prodanović, Radiša, Sladojević, Željko, Kirovski, Danijela, Vujanac, Ivan, Ivetić, V., Savić, Božidar, Kureljušić, Branislav, Stevančević, M., "Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows" in Biotechnology in Animal Husbandry, 28, no. 1 (2012):25-32,
https://doi.org/10.2298/BAH1201025P . .