TY  - JOUR
AU  - Sapundžić Zurovac, Zorana
AU  - Žutić, Jadranka
AU  - Stević, Nataša
AU  - Milićević, Vesna
AU  - Radojičić, Marina
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2510
AB  - Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.
PB  - MDPI
T2  - Veterinary Sciences
T1  - First Report of Brucella Seroprevalence in Wild Boar Population in Serbia
VL  - 9
IS  - 10
SP  - 575
DO  - 10.3390/vetsci9100575
ER  - 

@article{
author = "Sapundžić Zurovac, Zorana and Žutić, Jadranka and Stević, Nataša and Milićević, Vesna and Radojičić, Marina and Stanojević, Slavoljub and Radojičić, Sonja",
year = "2022",
abstract = "Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.",
publisher = "MDPI",
journal = "Veterinary Sciences",
title = "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia",
volume = "9",
number = "10",
pages = "575",
doi = "10.3390/vetsci9100575"
}

Sapundžić Zurovac, Z., Žutić, J., Stević, N., Milićević, V., Radojičić, M., Stanojević, S.,& Radojičić, S.. (2022). First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences
MDPI., 9(10), 575.
https://doi.org/10.3390/vetsci9100575

Sapundžić Zurovac Z, Žutić J, Stević N, Milićević V, Radojičić M, Stanojević S, Radojičić S. First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences. 2022;9(10):575.
doi:10.3390/vetsci9100575 .

Sapundžić Zurovac, Zorana, Žutić, Jadranka, Stević, Nataša, Milićević, Vesna, Radojičić, Marina, Stanojević, Slavoljub, Radojičić, Sonja, "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia" in Veterinary Sciences, 9, no. 10 (2022):575,
https://doi.org/10.3390/vetsci9100575 . .

TY  - CONF
AU  - Ninković, Milan
AU  - Žutić, Jadranka
AU  - Arsić, Sveta
AU  - Zdravković, Nemanja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2893
AB  - U vezanom sistemu držanja krava, bolest bele linije i čir papka se
izdvajaju kao vodeći zdravstveni problemi vezani za njihove ekstremitete. U većini slučajeva, čir papka se pojavljuje na zadnjim ekstremitetima, a retko na prednjim, Cilj ove
studije je bio da se analizira frekvenca pojave čira papka na prednjim ekstremitetima
kod krava simentaske rase. U našoj studiji je utvđeno da se čir papka na prednjm ekstremiretim pojavio kod 4 od 45 krava (8,9%). Ove lezije su zapažene i na zadnjim nogama
kod 8 od 45 krava (17,8%). Ukoliko su promene kod iste jedinke bile i na prednjim i na
zadnjim nogama, uglavnom su bile kontralateralne. Predisponirajući faktor za nastanak čira papka je bila vlažna prostrka od slame. Redovno i pravilno obrezivanje papaka, uz korekciju ambijentanih uslova su ključ za prevenciju pojave oboljenja papaka.
AB  - : In a tied holding systems, white line disease and sole ulcers stand out
as prominent health problems of the cows limbs. The most often sole ulcers occur on the
hind limbs, rarely on the fore limbs. The aim of this study was to provide information
on the frequency of sole ulcers on the fore limbs in Simmental cows. In our study, sole
ulcers were on the fore limbs found in 4/45 cows (8.9%). The lesions were also present
on the hind limbs 8/45 (17.8%) of examined cows. The distribution of sole ulcers on the
same animal were mostly contralateral. The presence of the humid straw bedding has
proven to be a predisposing factor for sole ulcers. Regular management of hoof trimmings, with improved ambient conditions, has key role in preventing occurrence of the
hoof disease.
PB  - Beograd : Udruženje veterinara praktičara Srbije
C3  - IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i repordukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022
T1  - Nalaz čira papaka na prednjim nogama kod krava u vezanom sistemu držanja
T1  - The findings of sole ulcers in the forelimbs of cows in tied holding system
SP  - 241
EP  - 243
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2893
ER  - 

@conference{
author = "Ninković, Milan and Žutić, Jadranka and Arsić, Sveta and Zdravković, Nemanja",
year = "2022",
abstract = "U vezanom sistemu držanja krava, bolest bele linije i čir papka se
izdvajaju kao vodeći zdravstveni problemi vezani za njihove ekstremitete. U većini slučajeva, čir papka se pojavljuje na zadnjim ekstremitetima, a retko na prednjim, Cilj ove
studije je bio da se analizira frekvenca pojave čira papka na prednjim ekstremitetima
kod krava simentaske rase. U našoj studiji je utvđeno da se čir papka na prednjm ekstremiretim pojavio kod 4 od 45 krava (8,9%). Ove lezije su zapažene i na zadnjim nogama
kod 8 od 45 krava (17,8%). Ukoliko su promene kod iste jedinke bile i na prednjim i na
zadnjim nogama, uglavnom su bile kontralateralne. Predisponirajući faktor za nastanak čira papka je bila vlažna prostrka od slame. Redovno i pravilno obrezivanje papaka, uz korekciju ambijentanih uslova su ključ za prevenciju pojave oboljenja papaka., : In a tied holding systems, white line disease and sole ulcers stand out
as prominent health problems of the cows limbs. The most often sole ulcers occur on the
hind limbs, rarely on the fore limbs. The aim of this study was to provide information
on the frequency of sole ulcers on the fore limbs in Simmental cows. In our study, sole
ulcers were on the fore limbs found in 4/45 cows (8.9%). The lesions were also present
on the hind limbs 8/45 (17.8%) of examined cows. The distribution of sole ulcers on the
same animal were mostly contralateral. The presence of the humid straw bedding has
proven to be a predisposing factor for sole ulcers. Regular management of hoof trimmings, with improved ambient conditions, has key role in preventing occurrence of the
hoof disease.",
publisher = "Beograd : Udruženje veterinara praktičara Srbije",
journal = "IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i repordukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022",
title = "Nalaz čira papaka na prednjim nogama kod krava u vezanom sistemu držanja, The findings of sole ulcers in the forelimbs of cows in tied holding system",
pages = "241-243",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2893"
}

Ninković, M., Žutić, J., Arsić, S.,& Zdravković, N.. (2022). Nalaz čira papaka na prednjim nogama kod krava u vezanom sistemu držanja. in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i repordukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022
Beograd : Udruženje veterinara praktičara Srbije., 241-243.
https://hdl.handle.net/21.15107/rcub_veterinar_2893

Ninković M, Žutić J, Arsić S, Zdravković N. Nalaz čira papaka na prednjim nogama kod krava u vezanom sistemu držanja. in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i repordukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022. 2022;:241-243.
https://hdl.handle.net/21.15107/rcub_veterinar_2893 .

Ninković, Milan, Žutić, Jadranka, Arsić, Sveta, Zdravković, Nemanja, "Nalaz čira papaka na prednjim nogama kod krava u vezanom sistemu držanja" in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i repordukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022 (2022):241-243,
https://hdl.handle.net/21.15107/rcub_veterinar_2893 .

TY  - CONF
AU  - Ninković, Milan
AU  - Žutić, Jadranka
AU  - Zdravković, Nemanja
AU  - Kureljušić, Branislav
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Bojkovski, Jovan
AU  - Nikolić, Marko
AU  - Arsić, Sveta
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2877
AB  - Долазак телади у товилишта повезан је са значајним променама
у исхрани и утицајем различитих фактора средине што се често
манифестује појавом здравствених проблема. Најчешће су ти
проблеми везани на поремећаје дигестивног и респираторног тракта.
Услед интеракције вируса, бактерија и стресогених фактора долази до
појаве дијареја и пнеумонија код телади. Бројни предиспонирајући
фактори као што су прелазак са млечне исхране на чврсту кабасту
храну, гладовање, жеђ, неадекватни микроклиматски услови,
неподесни и дуги транспорт, исцрпљеност, нагла промена исхране
и пренатрпаност у објектима погодују брзом развоју болести код
већег броја јединки у товилиштима. Наша истраживања спроведена
су у 3 услужна товилишта у Колубарском округу током месеца
марта 2022. године. Укупно је у сва три товилишта било смештено 185 мушке телади, сименталске расе, просечне старости од 2 до 4
месеца и телесне масе између 120-160 кг. Промене здраственог стања
телади праћене су првих 15 дана након доласка у товилишта. У том
периоду регистровани су симптоми поремећаја дигестивног тракта,
и то дијареја код 56/185 (30.3%) и надун код 3/185 (1.62%) телета.
Појава кашља је забележена код 95/185 (51.3%) грла, при чему је
код 18/185 (9.73%) телади дошло до поремећаја општег здраственог
стања, праћено повишеном телесном температуром, носним исцетком
и присутним абдоминалним дисањем. Угинућа нису забележена.
Обезбеђивање постепеног преласка млечне исхране на концентровану
кабасту храну, минимизирање стресогених фактора и спровођење
превентивних имунопрофилактичких мера представљају кључни
корак у заштити здравља телади и смањењу економских губитака.
AB  - The arrival of calves in fattening grounds is associated with significant
changes in diet and the influence of various environmental factors, which
are often manifested by the appearance of various health problems.
Most often, these problems are related to disorders of the digestive
and respiratory tract. Diarrhoea and pneumonia in calves occur due
to the interactions between viruses, bacteria and stressors. Numerous
predisposing factors such as the transition from milk to solid feed,
starvation, thirst, inadequate microclimatic conditions, unsuitable and
long transport, exhaustion, sudden changes in diet and overcrowding
in facilities affect the rapid development of the different diseases in
calves. This research was conducted in 3 service fattening farms in
Kolubara’s district, Republic of Serbia, during March 2022. A total
of 185 Simmental male calves, with an average age of 2 to 4 months and bodyweight between 120-160 kg, were housed in three different
fattening facilities. Changes in the health status of calves were monitored for the first 15 days after arrival at the fattening grounds. During
this period, the symptoms of digestive tract disorders were monitored
and recorded. Namely, diarrhoea was registered in 56/185 (30.3%) and
flatulence in 3/185 (1.6%) calves. The occurrence of cough was noticed
in 95/185 (51.3%) calves, while in 18/185(9.7%) calves there general
health disorders, followed by fever, nasal discharge and abdominal
breathing were present. No deaths were reported. Ensuring a gradual
transition from milk to concentrated feed, minimizing stressors and
implementing preventive immunoprophylaxis measures are key steps
in protecting calf health and reducing economic losses.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022
T1  - Najčešći poremećaji zdravstvenog stanja kod teladi u tovilištima
T1  - The most common health disorders of calves on fattening grounds
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2877
ER  - 

@conference{
author = "Ninković, Milan and Žutić, Jadranka and Zdravković, Nemanja and Kureljušić, Branislav and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Bojkovski, Jovan and Nikolić, Marko and Arsić, Sveta",
year = "2022",
abstract = "Долазак телади у товилишта повезан је са значајним променама
у исхрани и утицајем различитих фактора средине што се често
манифестује појавом здравствених проблема. Најчешће су ти
проблеми везани на поремећаје дигестивног и респираторног тракта.
Услед интеракције вируса, бактерија и стресогених фактора долази до
појаве дијареја и пнеумонија код телади. Бројни предиспонирајући
фактори као што су прелазак са млечне исхране на чврсту кабасту
храну, гладовање, жеђ, неадекватни микроклиматски услови,
неподесни и дуги транспорт, исцрпљеност, нагла промена исхране
и пренатрпаност у објектима погодују брзом развоју болести код
већег броја јединки у товилиштима. Наша истраживања спроведена
су у 3 услужна товилишта у Колубарском округу током месеца
марта 2022. године. Укупно је у сва три товилишта било смештено 185 мушке телади, сименталске расе, просечне старости од 2 до 4
месеца и телесне масе између 120-160 кг. Промене здраственог стања
телади праћене су првих 15 дана након доласка у товилишта. У том
периоду регистровани су симптоми поремећаја дигестивног тракта,
и то дијареја код 56/185 (30.3%) и надун код 3/185 (1.62%) телета.
Појава кашља је забележена код 95/185 (51.3%) грла, при чему је
код 18/185 (9.73%) телади дошло до поремећаја општег здраственог
стања, праћено повишеном телесном температуром, носним исцетком
и присутним абдоминалним дисањем. Угинућа нису забележена.
Обезбеђивање постепеног преласка млечне исхране на концентровану
кабасту храну, минимизирање стресогених фактора и спровођење
превентивних имунопрофилактичких мера представљају кључни
корак у заштити здравља телади и смањењу економских губитака., The arrival of calves in fattening grounds is associated with significant
changes in diet and the influence of various environmental factors, which
are often manifested by the appearance of various health problems.
Most often, these problems are related to disorders of the digestive
and respiratory tract. Diarrhoea and pneumonia in calves occur due
to the interactions between viruses, bacteria and stressors. Numerous
predisposing factors such as the transition from milk to solid feed,
starvation, thirst, inadequate microclimatic conditions, unsuitable and
long transport, exhaustion, sudden changes in diet and overcrowding
in facilities affect the rapid development of the different diseases in
calves. This research was conducted in 3 service fattening farms in
Kolubara’s district, Republic of Serbia, during March 2022. A total
of 185 Simmental male calves, with an average age of 2 to 4 months and bodyweight between 120-160 kg, were housed in three different
fattening facilities. Changes in the health status of calves were monitored for the first 15 days after arrival at the fattening grounds. During
this period, the symptoms of digestive tract disorders were monitored
and recorded. Namely, diarrhoea was registered in 56/185 (30.3%) and
flatulence in 3/185 (1.6%) calves. The occurrence of cough was noticed
in 95/185 (51.3%) calves, while in 18/185(9.7%) calves there general
health disorders, followed by fever, nasal discharge and abdominal
breathing were present. No deaths were reported. Ensuring a gradual
transition from milk to concentrated feed, minimizing stressors and
implementing preventive immunoprophylaxis measures are key steps
in protecting calf health and reducing economic losses.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022",
title = "Najčešći poremećaji zdravstvenog stanja kod teladi u tovilištima, The most common health disorders of calves on fattening grounds",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2877"
}

Ninković, M., Žutić, J., Zdravković, N., Kureljušić, B., Glišić, D., Zurovac Sapundžić, Z., Bojkovski, J., Nikolić, M.,& Arsić, S.. (2022). Najčešći poremećaji zdravstvenog stanja kod teladi u tovilištima. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022
Banja Luka : Veterinarska komora Republike Srpske..
https://hdl.handle.net/21.15107/rcub_veterinar_2877

Ninković M, Žutić J, Zdravković N, Kureljušić B, Glišić D, Zurovac Sapundžić Z, Bojkovski J, Nikolić M, Arsić S. Najčešći poremećaji zdravstvenog stanja kod teladi u tovilištima. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2877 .

Ninković, Milan, Žutić, Jadranka, Zdravković, Nemanja, Kureljušić, Branislav, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Bojkovski, Jovan, Nikolić, Marko, Arsić, Sveta, "Najčešći poremećaji zdravstvenog stanja kod teladi u tovilištima" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15-18. jun 2022 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2877 .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Aleksić-Kovačević, Sanja
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
AU  - Maksimović Zorić, Jelena
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Knežević, Đorđe
AU  - Spalević, Ljiljana
AU  - Kukolj, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2784
AB  - Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.
PB  - Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)
T2  - Veterinaria Italiana
T1  - Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
VL  - 54
IS  - 7
SP  - 287
EP  - 295
DO  - 10.12834/VetIt.1813.9553.3
ER  - 

@article{
author = "Kureljušić, Branislav and Aleksić-Kovačević, Sanja and Savić, Božidar and Prodanović, Radiša and Jezdimirović, Nemanja and Milićević, Vesna and Maksimović Zorić, Jelena and Kureljušić, Jasna and Žutić, Jadranka and Knežević, Đorđe and Spalević, Ljiljana and Kukolj, Vladimir",
year = "2021",
abstract = "Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.",
publisher = "Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)",
journal = "Veterinaria Italiana",
title = "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus",
volume = "54",
number = "7",
pages = "287-295",
doi = "10.12834/VetIt.1813.9553.3"
}

Kureljušić, B., Aleksić-Kovačević, S., Savić, B., Prodanović, R., Jezdimirović, N., Milićević, V., Maksimović Zorić, J., Kureljušić, J., Žutić, J., Knežević, Đ., Spalević, L.,& Kukolj, V.. (2021). Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)., 54(7), 287-295.
https://doi.org/10.12834/VetIt.1813.9553.3

Kureljušić B, Aleksić-Kovačević S, Savić B, Prodanović R, Jezdimirović N, Milićević V, Maksimović Zorić J, Kureljušić J, Žutić J, Knežević Đ, Spalević L, Kukolj V. Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana. 2021;54(7):287-295.
doi:10.12834/VetIt.1813.9553.3 .

Kureljušić, Branislav, Aleksić-Kovačević, Sanja, Savić, Božidar, Prodanović, Radiša, Jezdimirović, Nemanja, Milićević, Vesna, Maksimović Zorić, Jelena, Kureljušić, Jasna, Žutić, Jadranka, Knežević, Đorđe, Spalević, Ljiljana, Kukolj, Vladimir, "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus" in Veterinaria Italiana, 54, no. 7 (2021):287-295,
https://doi.org/10.12834/VetIt.1813.9553.3 . .

TY  - JOUR
AU  - Čobanović, Nikola
AU  - Stajković, Silvana
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Stanković, Sanja Dj
AU  - Karabasil, Nedjeljko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1993
AB  - This study examined the relationship between lung lesion severity and presence of antibodies of various respiratory pathogens, and the effects of lung lesion severity on growth performance, biochemical indicators, total aerobe counts, and carcass and meat quality indicators in total of 240 slaughter pigs originating from two farms with similar rearing conditions. Lung lesion severity was calculated based on the degree of pneumonia and pleurisy in slaughtered pigs. Two-step cluster analysis was used to place individual pigs to four clusters according to pneumonia and pleurisy scores: no lung lesions (cluster 1); mild lung lesions (cluster 2); moderate lung lesions (cluster 3); and severe lung lesions (cluster 4). ANOVA and post hoc pairwise comparisons using Tukey's test were performed to assess the differences between clusters in examined variables. Multivariate linear regression analysis was run to identify associations between lung lesions and examined variables. There was a strong evidence of association between the absence of lung lesions and increased albumin, sodium and chloride levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and decreased haptoglobin, CK and LDH levels. Also, pigs without lung lesions produced the highest percentage of red, firm and nonexudative pork. Pigs having severe lung lesions had the highest percentage of simultaneously seropositive samples to SIV, PRRSV, PCV-2, PRCV, APP and M. hyopneumoniae. There was a strong evidence of association between the presence of severe lung lesions and decreased lactate, glucose, sodium, chloride and albumine levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and increased CK, LDH and haptoglobin levels. There was a strong evidence of association between the presence of severe lung lesions in slaughered pigs and increased meat pH and sensory colour scores, and decreased drip, thawing and cooking losses and L* and b* values, which led to the highest occurrence of moderate DFD and DFD pork. There was a strong evidence of association between the presence of mild lung lesions in slaughtered pigs and decreased meat pH and sensory colour scores, and increased drip, thawing and cooking losses, L* and b* values, which led to the highest occurrence of moderate PSE and PSE pork. In conclusion, the presence of lung lesions, irrespective of severity, was significantly associated with alterations in the biochemical indicators, growth performance and carcass and meat quality in slaughtered pigs.
PB  - Elsevier
T2  - Preventive Veterinary Medicine
T1  - Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs
VL  - 188
DO  - 10.1016/j.prevetmed.2021.105269
ER  - 

@article{
author = "Čobanović, Nikola and Stajković, Silvana and Kureljušić, Jasna and Žutić, Jadranka and Kureljušić, Branislav and Stanković, Sanja Dj and Karabasil, Nedjeljko",
year = "2021",
abstract = "This study examined the relationship between lung lesion severity and presence of antibodies of various respiratory pathogens, and the effects of lung lesion severity on growth performance, biochemical indicators, total aerobe counts, and carcass and meat quality indicators in total of 240 slaughter pigs originating from two farms with similar rearing conditions. Lung lesion severity was calculated based on the degree of pneumonia and pleurisy in slaughtered pigs. Two-step cluster analysis was used to place individual pigs to four clusters according to pneumonia and pleurisy scores: no lung lesions (cluster 1); mild lung lesions (cluster 2); moderate lung lesions (cluster 3); and severe lung lesions (cluster 4). ANOVA and post hoc pairwise comparisons using Tukey's test were performed to assess the differences between clusters in examined variables. Multivariate linear regression analysis was run to identify associations between lung lesions and examined variables. There was a strong evidence of association between the absence of lung lesions and increased albumin, sodium and chloride levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and decreased haptoglobin, CK and LDH levels. Also, pigs without lung lesions produced the highest percentage of red, firm and nonexudative pork. Pigs having severe lung lesions had the highest percentage of simultaneously seropositive samples to SIV, PRRSV, PCV-2, PRCV, APP and M. hyopneumoniae. There was a strong evidence of association between the presence of severe lung lesions and decreased lactate, glucose, sodium, chloride and albumine levels, daily weight gain, live weight, hot carcass weight, cold carcass weight, loin thickness and carcass lean content, and increased CK, LDH and haptoglobin levels. There was a strong evidence of association between the presence of severe lung lesions in slaughered pigs and increased meat pH and sensory colour scores, and decreased drip, thawing and cooking losses and L* and b* values, which led to the highest occurrence of moderate DFD and DFD pork. There was a strong evidence of association between the presence of mild lung lesions in slaughtered pigs and decreased meat pH and sensory colour scores, and increased drip, thawing and cooking losses, L* and b* values, which led to the highest occurrence of moderate PSE and PSE pork. In conclusion, the presence of lung lesions, irrespective of severity, was significantly associated with alterations in the biochemical indicators, growth performance and carcass and meat quality in slaughtered pigs.",
publisher = "Elsevier",
journal = "Preventive Veterinary Medicine",
title = "Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs",
volume = "188",
doi = "10.1016/j.prevetmed.2021.105269"
}

Čobanović, N., Stajković, S., Kureljušić, J., Žutić, J., Kureljušić, B., Stanković, S. D.,& Karabasil, N.. (2021). Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs. in Preventive Veterinary Medicine
Elsevier., 188.
https://doi.org/10.1016/j.prevetmed.2021.105269

Čobanović N, Stajković S, Kureljušić J, Žutić J, Kureljušić B, Stanković SD, Karabasil N. Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs. in Preventive Veterinary Medicine. 2021;188.
doi:10.1016/j.prevetmed.2021.105269 .

Čobanović, Nikola, Stajković, Silvana, Kureljušić, Jasna, Žutić, Jadranka, Kureljušić, Branislav, Stanković, Sanja Dj, Karabasil, Nedjeljko, "Biochemical, carcass and meat quality alterations associated with different degree of lung lesions in slaughtered pigs" in Preventive Veterinary Medicine, 188 (2021),
https://doi.org/10.1016/j.prevetmed.2021.105269 . .

TY  - JOUR
AU  - Ninković, Milan
AU  - Arsić, Sveta
AU  - Žutić, Jadranka
AU  - Zdravković, Nemanja
AU  - Glišić, Dimitrije
AU  - Sapundžić Zurovac, Zorana
AU  - Bojkovski, Jovan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2578
AB  - Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.
PB  - Massimo Morgante
T2  - Large Animal Review
T1  - Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows
VL  - 27
IS  - 6
SP  - 329
EP  - 332
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2578
ER  - 

@article{
author = "Ninković, Milan and Arsić, Sveta and Žutić, Jadranka and Zdravković, Nemanja and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Bojkovski, Jovan",
year = "2021",
abstract = "Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.",
publisher = "Massimo Morgante",
journal = "Large Animal Review",
title = "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows",
volume = "27",
number = "6",
pages = "329-332",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2578"
}

Ninković, M., Arsić, S., Žutić, J., Zdravković, N., Glišić, D., Sapundžić Zurovac, Z.,& Bojkovski, J.. (2021). Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review
Massimo Morgante., 27(6), 329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578

Ninković M, Arsić S, Žutić J, Zdravković N, Glišić D, Sapundžić Zurovac Z, Bojkovski J. Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review. 2021;27(6):329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .

Ninković, Milan, Arsić, Sveta, Žutić, Jadranka, Zdravković, Nemanja, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Bojkovski, Jovan, "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows" in Large Animal Review, 27, no. 6 (2021):329-332,
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1796
AB  - A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.
PB  - Polish Soc Veterinary Sciences Editorial Office, Lublin
T2  - Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.",
publisher = "Polish Soc Veterinary Sciences Editorial Office, Lublin",
journal = "Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
Polish Soc Veterinary Sciences Editorial Office, Lublin., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2017
AB  - A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.
PB  - Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)
T2  - Medycyna Weterynaryjna
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.",
publisher = "Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)",
journal = "Medycyna Weterynaryjna",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna
Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Jasna
AU  - Milićević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Vesković Moračanin, Slavica
AU  - Žutić, Jadranka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1860
AB  - INTRODUCTION: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. METHODOLOGY: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. RESULTS: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). CONCLUSIONS: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.
PB  - NLM (Medline)
T2  - Journal of infection in developing countries
T1  - Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
VL  - 14
IS  - 6
SP  - 669
EP  - 673
DO  - 10.3855/jidc.12552
ER  - 

@article{
author = "Kureljušić, Branislav and Savić, Božidar and Jezdimirović, Nemanja and Kureljušić, Jasna and Milićević, Vesna and Karabasil, Nedjeljko and Vesković Moračanin, Slavica and Žutić, Jadranka",
year = "2020",
abstract = "INTRODUCTION: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. METHODOLOGY: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. RESULTS: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). CONCLUSIONS: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.",
publisher = "NLM (Medline)",
journal = "Journal of infection in developing countries",
title = "Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade",
volume = "14",
number = "6",
pages = "669-673",
doi = "10.3855/jidc.12552"
}

Kureljušić, B., Savić, B., Jezdimirović, N., Kureljušić, J., Milićević, V., Karabasil, N., Vesković Moračanin, S.,& Žutić, J.. (2020). Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade. in Journal of infection in developing countries
NLM (Medline)., 14(6), 669-673.
https://doi.org/10.3855/jidc.12552

Kureljušić B, Savić B, Jezdimirović N, Kureljušić J, Milićević V, Karabasil N, Vesković Moračanin S, Žutić J. Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade. in Journal of infection in developing countries. 2020;14(6):669-673.
doi:10.3855/jidc.12552 .

Kureljušić, Branislav, Savić, Božidar, Jezdimirović, Nemanja, Kureljušić, Jasna, Milićević, Vesna, Karabasil, Nedjeljko, Vesković Moračanin, Slavica, Žutić, Jadranka, "Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade" in Journal of infection in developing countries, 14, no. 6 (2020):669-673,
https://doi.org/10.3855/jidc.12552 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Maksimović-Zorić, Jelena
AU  - Savić, Božidar
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1985
AB  - Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia
VL  - 132
SP  - 404
EP  - 406
DO  - 10.1016/j.rvsc.2020.07.028
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Maksimović-Zorić, Jelena and Savić, Božidar and Spalević, Ljiljana and Žutić, Jadranka",
year = "2020",
abstract = "Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia",
volume = "132",
pages = "404-406",
doi = "10.1016/j.rvsc.2020.07.028"
}

Milićević, V., Kureljušić, B., Maksimović-Zorić, J., Savić, B., Spalević, L.,& Žutić, J.. (2020). Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science
Elsevier., 132, 404-406.
https://doi.org/10.1016/j.rvsc.2020.07.028

Milićević V, Kureljušić B, Maksimović-Zorić J, Savić B, Spalević L, Žutić J. Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science. 2020;132:404-406.
doi:10.1016/j.rvsc.2020.07.028 .

Milićević, Vesna, Kureljušić, Branislav, Maksimović-Zorić, Jelena, Savić, Božidar, Spalević, Ljiljana, Žutić, Jadranka, "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia" in Research in Veterinary Science, 132 (2020):404-406,
https://doi.org/10.1016/j.rvsc.2020.07.028 . .

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Krnjaić, Dejan
AU  - Radanović, Oliver
AU  - Žutić, Jadranka
AU  - Spalević, Ljiljana
AU  - Jovanović, Milijan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1748
AB  - The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus
VL  - 69
IS  - 2
SP  - 201
EP  - 217
DO  - 10.2478/acve-2019-0016
ER  - 

@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Ivetić, Vojin and Krnjaić, Dejan and Radanović, Oliver and Žutić, Jadranka and Spalević, Ljiljana and Jovanović, Milijan",
year = "2019",
abstract = "The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus",
volume = "69",
number = "2",
pages = "201-217",
doi = "10.2478/acve-2019-0016"
}

Jezdimirović, N., Kureljušić, B., Ivetić, V., Krnjaić, D., Radanović, O., Žutić, J., Spalević, L.,& Jovanović, M.. (2019). Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 201-217.
https://doi.org/10.2478/acve-2019-0016

Jezdimirović N, Kureljušić B, Ivetić V, Krnjaić D, Radanović O, Žutić J, Spalević L, Jovanović M. Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd. 2019;69(2):201-217.
doi:10.2478/acve-2019-0016 .

Jezdimirović, Nemanja, Kureljušić, Branislav, Ivetić, Vojin, Krnjaić, Dejan, Radanović, Oliver, Žutić, Jadranka, Spalević, Ljiljana, Jovanović, Milijan, "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus" in Acta Veterinaria-Beograd, 69, no. 2 (2019):201-217,
https://doi.org/10.2478/acve-2019-0016 . .

TY  - CONF
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Savić Radovanović, Radoslava
AU  - Žutić, Jadranka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2886
AB  - Проналазак антимикробних лекова сврстава се у десет највећих подухвата
људског рода XX века. На тему лечења инфекција интересовање за одабир
адекватних активних супстанци за спровођење терапије не јењава од овог
историјског почетка, а нажалост, врло брзо након увођења нових препарата
описивана је и резистенција према истим. Заправо, интересовање за
сазнањима и распрострањеношћу резистентних бактерија је повећано
увођењем система једног здравља и организованог надзора на осетљивост и
резистенцију према антимимикробним лековима. Адекватна примена лечења
и одабира лекова у терапији почиње од тумачења антибиограма.
Антибиограм представља профил антимикробне осетљивости одређеног
изолата, на основу кога се у сваком појединачном случају, врши избор
антибиотика за терапирање. Избор антибиотика за оређени изолат најчешће је
приказан у стандардима за антимикробну терапију. Најпознатији стандари
проистичу из великих комитета као што су Европски (EUCAST) или Амерички
(CLSI) а постоје и други, национални стандарди одређених држава (нпр.
Француска или Велика Британија). На основу резултата већег броја
истраживања долази се до тога који антибиотици се у датом случају могу
проценити као повољан а који као неповољан избор на основу изолата и
патолошког материјала достављеног на анализу.
Најчешће грешке при емпиријском лечењу инфекција најчешће леже у
примени неадекватног лека који не делује на узрочника инфекције или делује
незадовољавајуће. Из овог разлога је потребно скренути пажњу на адекватно
тумачење антибиограма.
AB  - The finding of antimicrobials is ranked among the ten greatest achievements of
the human race of the 20th century. Since historical beginning, the interest in
selecting adequate active substances for the implementation of infection therapy
and unfortunately, very soon after the introduction of new principles, the
resistance to them was described. In fact, the interest in the knowledge and the
prevalence of resistant bacteria has been increased by the introduction of a OneHealth system and the organized monitoring of susceptibility and resistance of
bacteria toward antimicrobial drugs. Adequate application of treatment and
selection of drugs in therapy begins with the interpretation of antibiotibigram.
Antibiogram is the profile of the antimicrobial sensitivity of a particular isolate,
based on in each individual case, antibiotic therapy is chosen. The selection of
antibiotic for particular isolate is most often shown in the standards for
antimicrobial therapy. The most well-known standards come from large
committees such as European (EUCAST) or American (CLSI), and there are other,
national standards of certain countries (e.g. France or Great Britain). Based on the
results of a large number of studies, it comes to what antibiotics can be
administered in the given case and which are an unfavorable choice based on
isolates and pathological material submitted for analysis.
The most common mistakes in the empirical treatment of infections are most
often found in the use of an inadequate drug that does not act on the cause of the
infection or its action is unsatisfactory. For this reason, it is necessary to pay
attention to the adequate interpretation of the antibiogram.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
T1  - Antibiogram u maloj veterinarskoj praksi
T1  - Antibiogram in small veterinary practice
SP  - 243
EP  - 244
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2886
ER  - 

@conference{
author = "Zdravković, Nemanja and Radanović, Oliver and Savić Radovanović, Radoslava and Žutić, Jadranka",
year = "2019",
abstract = "Проналазак антимикробних лекова сврстава се у десет највећих подухвата
људског рода XX века. На тему лечења инфекција интересовање за одабир
адекватних активних супстанци за спровођење терапије не јењава од овог
историјског почетка, а нажалост, врло брзо након увођења нових препарата
описивана је и резистенција према истим. Заправо, интересовање за
сазнањима и распрострањеношћу резистентних бактерија је повећано
увођењем система једног здравља и организованог надзора на осетљивост и
резистенцију према антимимикробним лековима. Адекватна примена лечења
и одабира лекова у терапији почиње од тумачења антибиограма.
Антибиограм представља профил антимикробне осетљивости одређеног
изолата, на основу кога се у сваком појединачном случају, врши избор
антибиотика за терапирање. Избор антибиотика за оређени изолат најчешће је
приказан у стандардима за антимикробну терапију. Најпознатији стандари
проистичу из великих комитета као што су Европски (EUCAST) или Амерички
(CLSI) а постоје и други, национални стандарди одређених држава (нпр.
Француска или Велика Британија). На основу резултата већег броја
истраживања долази се до тога који антибиотици се у датом случају могу
проценити као повољан а који као неповољан избор на основу изолата и
патолошког материјала достављеног на анализу.
Најчешће грешке при емпиријском лечењу инфекција најчешће леже у
примени неадекватног лека који не делује на узрочника инфекције или делује
незадовољавајуће. Из овог разлога је потребно скренути пажњу на адекватно
тумачење антибиограма., The finding of antimicrobials is ranked among the ten greatest achievements of
the human race of the 20th century. Since historical beginning, the interest in
selecting adequate active substances for the implementation of infection therapy
and unfortunately, very soon after the introduction of new principles, the
resistance to them was described. In fact, the interest in the knowledge and the
prevalence of resistant bacteria has been increased by the introduction of a OneHealth system and the organized monitoring of susceptibility and resistance of
bacteria toward antimicrobial drugs. Adequate application of treatment and
selection of drugs in therapy begins with the interpretation of antibiotibigram.
Antibiogram is the profile of the antimicrobial sensitivity of a particular isolate,
based on in each individual case, antibiotic therapy is chosen. The selection of
antibiotic for particular isolate is most often shown in the standards for
antimicrobial therapy. The most well-known standards come from large
committees such as European (EUCAST) or American (CLSI), and there are other,
national standards of certain countries (e.g. France or Great Britain). Based on the
results of a large number of studies, it comes to what antibiotics can be
administered in the given case and which are an unfavorable choice based on
isolates and pathological material submitted for analysis.
The most common mistakes in the empirical treatment of infections are most
often found in the use of an inadequate drug that does not act on the cause of the
infection or its action is unsatisfactory. For this reason, it is necessary to pay
attention to the adequate interpretation of the antibiogram.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.",
title = "Antibiogram u maloj veterinarskoj praksi, Antibiogram in small veterinary practice",
pages = "243-244",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2886"
}

Zdravković, N., Radanović, O., Savić Radovanović, R.,& Žutić, J.. (2019). Antibiogram u maloj veterinarskoj praksi. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.
Banja Luka : Veterinarska komora Republike Srpske., 243-244.
https://hdl.handle.net/21.15107/rcub_veterinar_2886

Zdravković N, Radanović O, Savić Radovanović R, Žutić J. Antibiogram u maloj veterinarskoj praksi. in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019.. 2019;:243-244.
https://hdl.handle.net/21.15107/rcub_veterinar_2886 .

Zdravković, Nemanja, Radanović, Oliver, Savić Radovanović, Radoslava, Žutić, Jadranka, "Antibiogram u maloj veterinarskoj praksi" in 24. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), međunarodni naučni skup, Bijeljina, 12-15. jun 2019. (2019):243-244,
https://hdl.handle.net/21.15107/rcub_veterinar_2886 .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Maksimović, Slobodan
AU  - Vujinović, Slobodan
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Jezdimirović, Nemanja
AU  - Žutić, Jadranka
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1901
AB  - U ovom radu prikazan je slučaj krave simentalske rase, starosti dve godine i šest meseci kod koje je postojala sumnja na nodularni dermatitis, sredinom 2017 godine. Kliničkim pregledom ustanovljeni su brojni noduli veličine od nekoliko milimetara do približno 10 centimetara diseminovani dominantno po koži vimena i perineuma, od kojih su neki konfluirali i egzulcerisali. Opšte stanje krave je bilo nepromenjeno. Krava je prema rezultatima laboratorijskih analiza bila negativna na prisustvo antitela protiv virusa leukoze goveda, pokazala je negativnu reakciju pri tuberkulinizaciji i bila je vakcinisana protiv nodularnog dermatitisa. Nakon hirurške ekscizije kožnog čvora, uzorak je bio negativan na prisustvo genoma virusa nodularnog dermatitisa. Kako bismo ustanovili morfologiju kožne lezije, urađena je histopatološka analiza. Histopatološka analiza je pokazala infiltraciju dermisa i subkutisa brojnim mononukleranim ćelijama koje pokazuju ćelijsku atipiju. Tada je postavljena sumnja da se radi o kutanom limfomu. Nadalje, imunohistohemijsko ispitivanje je potvrdilo da se u infiltratu nalaze isključivo CD3 imunopozitivne ćelije što sugeriše da se radi o T-ćelijskom neepiteliotropnom limfomu
AB  - In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.
PB  - FVM
T2  - Veterinarski Glasnik
T1  - Cutaneous lymphoma in a cow - a case report
T1  - Kutani limfom kod krave – prikaz slučaja
VL  - 73
IS  - 1
SP  - 50
EP  - 56
DO  - 10.2298/VETGL180904004K
ER  - 

@article{
author = "Kureljušić, Branislav and Maksimović, Slobodan and Vujinović, Slobodan and Savić, Božidar and Milićević, Vesna and Veljović, Ljubiša and Jezdimirović, Nemanja and Žutić, Jadranka",
year = "2019",
abstract = "U ovom radu prikazan je slučaj krave simentalske rase, starosti dve godine i šest meseci kod koje je postojala sumnja na nodularni dermatitis, sredinom 2017 godine. Kliničkim pregledom ustanovljeni su brojni noduli veličine od nekoliko milimetara do približno 10 centimetara diseminovani dominantno po koži vimena i perineuma, od kojih su neki konfluirali i egzulcerisali. Opšte stanje krave je bilo nepromenjeno. Krava je prema rezultatima laboratorijskih analiza bila negativna na prisustvo antitela protiv virusa leukoze goveda, pokazala je negativnu reakciju pri tuberkulinizaciji i bila je vakcinisana protiv nodularnog dermatitisa. Nakon hirurške ekscizije kožnog čvora, uzorak je bio negativan na prisustvo genoma virusa nodularnog dermatitisa. Kako bismo ustanovili morfologiju kožne lezije, urađena je histopatološka analiza. Histopatološka analiza je pokazala infiltraciju dermisa i subkutisa brojnim mononukleranim ćelijama koje pokazuju ćelijsku atipiju. Tada je postavljena sumnja da se radi o kutanom limfomu. Nadalje, imunohistohemijsko ispitivanje je potvrdilo da se u infiltratu nalaze isključivo CD3 imunopozitivne ćelije što sugeriše da se radi o T-ćelijskom neepiteliotropnom limfomu, In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.",
publisher = "FVM",
journal = "Veterinarski Glasnik",
title = "Cutaneous lymphoma in a cow - a case report, Kutani limfom kod krave – prikaz slučaja",
volume = "73",
number = "1",
pages = "50-56",
doi = "10.2298/VETGL180904004K"
}

Kureljušić, B., Maksimović, S., Vujinović, S., Savić, B., Milićević, V., Veljović, L., Jezdimirović, N.,& Žutić, J.. (2019). Cutaneous lymphoma in a cow - a case report. in Veterinarski Glasnik
FVM., 73(1), 50-56.
https://doi.org/10.2298/VETGL180904004K

Kureljušić B, Maksimović S, Vujinović S, Savić B, Milićević V, Veljović L, Jezdimirović N, Žutić J. Cutaneous lymphoma in a cow - a case report. in Veterinarski Glasnik. 2019;73(1):50-56.
doi:10.2298/VETGL180904004K .

Kureljušić, Branislav, Maksimović, Slobodan, Vujinović, Slobodan, Savić, Božidar, Milićević, Vesna, Veljović, Ljubiša, Jezdimirović, Nemanja, Žutić, Jadranka, "Cutaneous lymphoma in a cow - a case report" in Veterinarski Glasnik, 73, no. 1 (2019):50-56,
https://doi.org/10.2298/VETGL180904004K . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Radosavljević, Vladimir
AU  - Jezdimirović, Nemanja
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1564
AB  - Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever.
AB  - Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Some adverse events following immunization in veterinary medicine
T1  - Procena neželjenih reakcija nakon vakcinacije u veterini
VL  - 11
IS  - 1
SP  - 67
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1564
ER  - 

@article{
author = "Milićević, Vesna and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Žutić, Jadranka and Kureljušić, Branislav and Radosavljević, Vladimir and Jezdimirović, Nemanja and Radojičić, Sonja",
year = "2018",
abstract = "Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever., Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Some adverse events following immunization in veterinary medicine, Procena neželjenih reakcija nakon vakcinacije u veterini",
volume = "11",
number = "1",
pages = "67-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1564"
}

Milićević, V., Veljović, L., Maksimović-Zorić, J., Žutić, J., Kureljušić, B., Radosavljević, V., Jezdimirović, N.,& Radojičić, S.. (2018). Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 11(1), 67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564

Milićević V, Veljović L, Maksimović-Zorić J, Žutić J, Kureljušić B, Radosavljević V, Jezdimirović N, Radojičić S. Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine. 2018;11(1):67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

Milićević, Vesna, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Žutić, Jadranka, Kureljušić, Branislav, Radosavljević, Vladimir, Jezdimirović, Nemanja, Radojičić, Sonja, "Some adverse events following immunization in veterinary medicine" in Arhiv veterinarske medicine, 11, no. 1 (2018):67-77,
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

TY  - JOUR
AU  - Vojinović, Dragica
AU  - Zdravković, Nemanja
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Nedić, S.
AU  - Giadinis, Nektarios
AU  - Panousis, N.
AU  - Manić, M.
AU  - Bugarski, Dejan
AU  - Palamarević, M.
AU  - Bogićević, Nataša
AU  - Dobrosavljević, Ivan
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
AU  - Prodanov-Radulović, Jasna
AU  - Bojkovski, Jovan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1613
AB  - Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia
VL  - 69
IS  - 4
SP  - 1241
EP  - 1245
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1613
ER  - 

@article{
author = "Vojinović, Dragica and Zdravković, Nemanja and Prodanović, Radiša and Vujanac, Ivan and Nedić, S. and Giadinis, Nektarios and Panousis, N. and Manić, M. and Bugarski, Dejan and Palamarević, M. and Bogićević, Nataša and Dobrosavljević, Ivan and Spalević, Ljiljana and Žutić, Jadranka and Prodanov-Radulović, Jasna and Bojkovski, Jovan",
year = "2018",
abstract = "Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M bovis in the blood serum of grazing daily cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia",
volume = "69",
number = "4",
pages = "1241-1245",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1613"
}

Vojinović, D., Zdravković, N., Prodanović, R., Vujanac, I., Nedić, S., Giadinis, N., Panousis, N., Manić, M., Bugarski, D., Palamarević, M., Bogićević, N., Dobrosavljević, I., Spalević, L., Žutić, J., Prodanov-Radulović, J.,& Bojkovski, J.. (2018). Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(4), 1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613

Vojinović D, Zdravković N, Prodanović R, Vujanac I, Nedić S, Giadinis N, Panousis N, Manić M, Bugarski D, Palamarević M, Bogićević N, Dobrosavljević I, Spalević L, Žutić J, Prodanov-Radulović J, Bojkovski J. Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia. in Journal of The Hellenic Veterinary Medical Society. 2018;69(4):1241-1245.
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

Vojinović, Dragica, Zdravković, Nemanja, Prodanović, Radiša, Vujanac, Ivan, Nedić, S., Giadinis, Nektarios, Panousis, N., Manić, M., Bugarski, Dejan, Palamarević, M., Bogićević, Nataša, Dobrosavljević, Ivan, Spalević, Ljiljana, Žutić, Jadranka, Prodanov-Radulović, Jasna, Bojkovski, Jovan, "Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia" in Journal of The Hellenic Veterinary Medical Society, 69, no. 4 (2018):1241-1245,
https://hdl.handle.net/21.15107/rcub_veterinar_1613 .

TY  - JOUR
AU  - Bacić, Dragan
AU  - Obrenović, Sonja
AU  - Dimitrijević, B.
AU  - Jonić, B.
AU  - Žutić, Jadranka
AU  - Ašanin, N.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/944
AB  - In this study, the protective effects of two bivalent inactivated vaccines were evaluated. Vaccines were prepared from Listeria monocytogenes, serotypes 1/2a and 4b, as the most frequent in our and surrounding epidemiological areas. Vaccine A consists of whole L. monocytogenes bacteria cells, inactivated with 0.4% formaldehyde and aluminium hydroxide as a carrier. Vaccine B contains 0.1% saponin in addition to ingredients of vaccine A. Evaluations of these vaccines were performed in 60 sheep, divided into four groups (n=10) with a corresponding negative control group (n=5). After 14 days, boosterisation of all animals was performed. In order to evaluate the immune response, blood samples were obtained every 14 days during the next 6 months. Antibody titres were determined by microaglutitation (MAT) and complement fixation tests (CFT). Comparative analyses of antibody titres, induced by vaccines A and B, show that the latter (with saponine) significantly increased the level of antibody titres (p lt 0.01). The levels of immune response were also significantly impacted by the total number of bacteria and vaccine dosage (p lt 0.01). The bivalent vaccine containing 0.1% saponin (vaccine B) in 5.0 mLx 106 cfu/mL (colony-forming units per milliliter) dosage shows a protective effect after challenge with L. monocytogenes. The protective levels of this antibody were 1/80 and 1/16, determined by MAT and CFT, respectively. Antibody titres were significantly higher after boosterisation (p lt 0.01) and protective levels could be detected in the sera of vaccinated animals during the next 6 months. Therefore, it is strongly recommended to perform boosterisation two weeks after the initial vaccination.
AB  - U ovom radu ispitivano je protektivno dejstvo eksperimentalno inaktivisane dvovalentne vakcine protiv listerioze ovaca.Vakcina je pripremana od sojeva L. monocytogenes 1/2a i 4b koji su najzastupljeniji na našem epizootiološkom području i u zemljama u okruženju. Vakcina A sadrži cele bakterijske ćelije koje su inaktivisane 0,4% formalinom uz dodatak aluminijum hidroksida kao nosača. Vakcina B pored gore navedenih sastojaka sadrži i 0,1% saponina. Ispitivanje ovako pripremljene eksperimentalne inaktivisane bivalentne vakcine protiv listerioze ovaca izvedeno je na 60 ovaca podeljenih u 4 grupe (n=10), pri čemu je svaka grupa imala kontrolnu grupu (n=5). Nakon 14 dana urađena je revakcinacija svih oglednih životinja. Krv je uzorkovana svakih 14 dana, tokom narednih 6 meseci i praćeno je kretanje titra antitela, metodom spore aglutinacije (MAT) i reakcijom vezivanja komplementa (RVK). Uporednim ispitivanjem visine titra antitela kod životinja koje su imunizovane vakcinom bez saponina i vakcinom sa 0,1% saponina ustanovljeno je da saponin značajno podstiče imunski odgovor. Ustanovljeno je da ukupan broj mikrooganizama u vakcini, kao i doza vakcine, utiču na kvalitet imunskog odgovora. Utvrđen je viši titar antitela ako se aplikuje doza vakcine od 5,0 ml × 106 cfu/ml nego kada je aplikovana doza od 2,5 ml × 106 cfu/ml (p lt 0,01). Dvovalentna vakcina pripremljena od inaktivisanih serotipova listerija sa saponinom u dozi od 5,0 ml × 106ml štitila je jagnjad od veštačke infekcije, a protektivni titar iznosio je 1:80 utvrđen metodom mikroaglutinacije, odnosno 1:16 metodom reakcije vezivanja komlementa. Titri antitela nakon revakcinacije su značajno viši nego posle prve vakcinacije (p,01) i mogli su da se otkriju u serumu životinja 6 meseci nakon vakcinacije, zbog čega se preporučuje obavezna revakcinacija 2 nedelje nakon vakcinacije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - A study on the inactivated bivalent vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes for the control of listeriosis in sheep
T1  - Ispitivanje inaktivisane dvovalentne vakcine pripremljene od serotipova 1/2a i 4b Listeria monocytogenes u kontroli listerioze kod ovaca
VL  - 62
IS  - 5-6
SP  - 531
EP  - 541
DO  - 10.2298/AVB1206531B
ER  - 

@article{
author = "Bacić, Dragan and Obrenović, Sonja and Dimitrijević, B. and Jonić, B. and Žutić, Jadranka and Ašanin, N.",
year = "2012",
abstract = "In this study, the protective effects of two bivalent inactivated vaccines were evaluated. Vaccines were prepared from Listeria monocytogenes, serotypes 1/2a and 4b, as the most frequent in our and surrounding epidemiological areas. Vaccine A consists of whole L. monocytogenes bacteria cells, inactivated with 0.4% formaldehyde and aluminium hydroxide as a carrier. Vaccine B contains 0.1% saponin in addition to ingredients of vaccine A. Evaluations of these vaccines were performed in 60 sheep, divided into four groups (n=10) with a corresponding negative control group (n=5). After 14 days, boosterisation of all animals was performed. In order to evaluate the immune response, blood samples were obtained every 14 days during the next 6 months. Antibody titres were determined by microaglutitation (MAT) and complement fixation tests (CFT). Comparative analyses of antibody titres, induced by vaccines A and B, show that the latter (with saponine) significantly increased the level of antibody titres (p lt 0.01). The levels of immune response were also significantly impacted by the total number of bacteria and vaccine dosage (p lt 0.01). The bivalent vaccine containing 0.1% saponin (vaccine B) in 5.0 mLx 106 cfu/mL (colony-forming units per milliliter) dosage shows a protective effect after challenge with L. monocytogenes. The protective levels of this antibody were 1/80 and 1/16, determined by MAT and CFT, respectively. Antibody titres were significantly higher after boosterisation (p lt 0.01) and protective levels could be detected in the sera of vaccinated animals during the next 6 months. Therefore, it is strongly recommended to perform boosterisation two weeks after the initial vaccination., U ovom radu ispitivano je protektivno dejstvo eksperimentalno inaktivisane dvovalentne vakcine protiv listerioze ovaca.Vakcina je pripremana od sojeva L. monocytogenes 1/2a i 4b koji su najzastupljeniji na našem epizootiološkom području i u zemljama u okruženju. Vakcina A sadrži cele bakterijske ćelije koje su inaktivisane 0,4% formalinom uz dodatak aluminijum hidroksida kao nosača. Vakcina B pored gore navedenih sastojaka sadrži i 0,1% saponina. Ispitivanje ovako pripremljene eksperimentalne inaktivisane bivalentne vakcine protiv listerioze ovaca izvedeno je na 60 ovaca podeljenih u 4 grupe (n=10), pri čemu je svaka grupa imala kontrolnu grupu (n=5). Nakon 14 dana urađena je revakcinacija svih oglednih životinja. Krv je uzorkovana svakih 14 dana, tokom narednih 6 meseci i praćeno je kretanje titra antitela, metodom spore aglutinacije (MAT) i reakcijom vezivanja komplementa (RVK). Uporednim ispitivanjem visine titra antitela kod životinja koje su imunizovane vakcinom bez saponina i vakcinom sa 0,1% saponina ustanovljeno je da saponin značajno podstiče imunski odgovor. Ustanovljeno je da ukupan broj mikrooganizama u vakcini, kao i doza vakcine, utiču na kvalitet imunskog odgovora. Utvrđen je viši titar antitela ako se aplikuje doza vakcine od 5,0 ml × 106 cfu/ml nego kada je aplikovana doza od 2,5 ml × 106 cfu/ml (p lt 0,01). Dvovalentna vakcina pripremljena od inaktivisanih serotipova listerija sa saponinom u dozi od 5,0 ml × 106ml štitila je jagnjad od veštačke infekcije, a protektivni titar iznosio je 1:80 utvrđen metodom mikroaglutinacije, odnosno 1:16 metodom reakcije vezivanja komlementa. Titri antitela nakon revakcinacije su značajno viši nego posle prve vakcinacije (p,01) i mogli su da se otkriju u serumu životinja 6 meseci nakon vakcinacije, zbog čega se preporučuje obavezna revakcinacija 2 nedelje nakon vakcinacije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "A study on the inactivated bivalent vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes for the control of listeriosis in sheep, Ispitivanje inaktivisane dvovalentne vakcine pripremljene od serotipova 1/2a i 4b Listeria monocytogenes u kontroli listerioze kod ovaca",
volume = "62",
number = "5-6",
pages = "531-541",
doi = "10.2298/AVB1206531B"
}

Bacić, D., Obrenović, S., Dimitrijević, B., Jonić, B., Žutić, J.,& Ašanin, N.. (2012). A study on the inactivated bivalent vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes for the control of listeriosis in sheep. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 531-541.
https://doi.org/10.2298/AVB1206531B

Bacić D, Obrenović S, Dimitrijević B, Jonić B, Žutić J, Ašanin N. A study on the inactivated bivalent vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes for the control of listeriosis in sheep. in Acta Veterinaria-Beograd. 2012;62(5-6):531-541.
doi:10.2298/AVB1206531B .

Bacić, Dragan, Obrenović, Sonja, Dimitrijević, B., Jonić, B., Žutić, Jadranka, Ašanin, N., "A study on the inactivated bivalent vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes for the control of listeriosis in sheep" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):531-541,
https://doi.org/10.2298/AVB1206531B . .

TY  - JOUR
AU  - Stanojković, Aleksandar
AU  - Ašanin, Ružica
AU  - Ašanin, Jelena
AU  - Palić, Ksenija
AU  - Stanojković, Aleksandra
AU  - Žutić, Jadranka
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1931
AB  - Cilj ovog rada je bilo ispitivanje prisustva i zastupljenosti streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od zdravih, zaklanih i uginulih svinja različitih kategorija sa nekoliko farmi i klanica u Republici Srbiji. Ukupno je ispitano 226 uzoraka briseva tonzila i nosa od klinički zdrave prasadi u tovu, briseva tonzila prasadi starosti 5-20 dana, delova nazofaringealnih tonzila tovljenika zaklanih na klanici, delova nazofaringealnih tonzila prasadi zaklanih na klanici, briseva sa trupova zaklanih svinja na klanici, briseva sa no`eva za evisceraciju organa na klanici, kao i briseva pluća, trbušne duplje i organa naglo uginule prasadi. Ispitivanje prisustva navedenih vrsta bakterija vršeno je primenom standardnih mikrobioloških metoda. Za identifikaciju izolovanih vrsta bakterija korišćeni su komercijalni biohemijski testovi, a za utvrđivanje serotipske pripadnosti izolovanih sojeva Streptococcus suis specifični serumi za kapsularne antigene. Ustanovljeno je da najveći broj izolovanih sojeva pripada rodu Streptococcus, a u daleko manjem broju izolovane bakterije su pripadale rodovima: Enterococcus (6) (10,4%), Aerococcus (3) (6,2%), Lactococcus (2) (4,2%) i Globicatella (2) (4,2%). Od navede nog broja Streptococcus vrsta najveći broj je pripadao vrsti Streptococcus suis (23) (64%). U brisevima nazofaringealnih tonzila utvrđeno je prisustvo i ostalih á hemolitičkih streptokoka: Streptococcus sanguinis (13,8%), Streptococcus salivarius (5,6%), Streptococcus mitis (5,6%), Streptococcus parasanguinis (2,7%) i Streptococcus oralis (2,7%). Takođe, u manjem procentu izolovana je i vrsta Streptococcus bovis (5,6%). Najveći procenat izolovanih bakterija iz roda Enterococcus pripadao je vrsti Enterococcus faecalis (80%), dok je vrsti Enterococcus faecium pripadao znatno manji procenat izolata (20%). Od ostalih takozvanih streptokokama sličnih vrsta bakterija izolovane su: Aerococcus viridans, Globicatella sanguinis i Lactococcus lactis ssp. cremoris.
AB  - The aim of this investigation was to establish the presence and prevalence of streptococci, enterococci and streptococci-like bacteria in various materials originating from healthy, slaughtered and dead pigs belonging to different categories from several farms and slaughterhouses in the Republic of Serbia. The total number of investigated samples comprised 226 swabs of tonsils and noses from clinically healthy breeders, swabs of tonsils from piglets 5-20 days old, parts of nasopharyngeal tonsils from breeders slaughtered in a slaughterhouse, parts of nasopharyngeal tonsils from piglets slaughtered in a slaughterhouse, swabs of slaughtered pig carcasses from a slaughterhouse, swabs from knives for evisceration in a slaughterhouse, as well as swabs of lungs, abdominal cavity and organs from piglets which died suddenly. The standard microbiological methods were used for investigations of the presence of the listed microorganisms. Commercial biochemical tests were used for the identification of the isolated bacteria and specific sera for capsular antigenes were used for serological determination of the isolated S. suis strains. It was established that the great majority of the isolated strains belonged to the genus Streptococcus (36) (75%), and the minority of the strains belonged to the following genera: Enterococcus (6) (10.4%), Aerococcus (3) (6.2%), Lactococcus (2) (4.2%) and Globicatella (2) (4.2%). The great majority of Streptococcus species belonged to S. suis. The presence of other á haemolytic streptococci was established in the swabs of nasopharyngeal tonsils: Streptococcus sanguinis (13.8%), Streptococcus salivarius (5.6%), Streptococcus mitis (5.6%), Streptococcus parasanguinis (2.7%) and Streptococcus oralis (2.7%). Also, S. bovis was isolated in a smaller percentage (5.6%). The greatest number of isolated bacteria from the genus Enterococcus belonged to Enterococcus faecalis (80%), while the minority of isolated strains belonged to Enterococcus faecium (20%). The following from the streptococci-like bacteria were isolated: Aerococcus viridans, Globicatella sanguinis and Lactococcus lactis ssp.cremoris.
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs
T1  - Ispitivanje prisustva alfa hemolitičkih streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od svinja
VL  - 65
IS  - 3-4
SP  - 203
EP  - 213
DO  - 10.2298/VETGL1104203S
ER  - 

@article{
author = "Stanojković, Aleksandar and Ašanin, Ružica and Ašanin, Jelena and Palić, Ksenija and Stanojković, Aleksandra and Žutić, Jadranka",
year = "2011",
abstract = "Cilj ovog rada je bilo ispitivanje prisustva i zastupljenosti streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od zdravih, zaklanih i uginulih svinja različitih kategorija sa nekoliko farmi i klanica u Republici Srbiji. Ukupno je ispitano 226 uzoraka briseva tonzila i nosa od klinički zdrave prasadi u tovu, briseva tonzila prasadi starosti 5-20 dana, delova nazofaringealnih tonzila tovljenika zaklanih na klanici, delova nazofaringealnih tonzila prasadi zaklanih na klanici, briseva sa trupova zaklanih svinja na klanici, briseva sa no`eva za evisceraciju organa na klanici, kao i briseva pluća, trbušne duplje i organa naglo uginule prasadi. Ispitivanje prisustva navedenih vrsta bakterija vršeno je primenom standardnih mikrobioloških metoda. Za identifikaciju izolovanih vrsta bakterija korišćeni su komercijalni biohemijski testovi, a za utvrđivanje serotipske pripadnosti izolovanih sojeva Streptococcus suis specifični serumi za kapsularne antigene. Ustanovljeno je da najveći broj izolovanih sojeva pripada rodu Streptococcus, a u daleko manjem broju izolovane bakterije su pripadale rodovima: Enterococcus (6) (10,4%), Aerococcus (3) (6,2%), Lactococcus (2) (4,2%) i Globicatella (2) (4,2%). Od navede nog broja Streptococcus vrsta najveći broj je pripadao vrsti Streptococcus suis (23) (64%). U brisevima nazofaringealnih tonzila utvrđeno je prisustvo i ostalih á hemolitičkih streptokoka: Streptococcus sanguinis (13,8%), Streptococcus salivarius (5,6%), Streptococcus mitis (5,6%), Streptococcus parasanguinis (2,7%) i Streptococcus oralis (2,7%). Takođe, u manjem procentu izolovana je i vrsta Streptococcus bovis (5,6%). Najveći procenat izolovanih bakterija iz roda Enterococcus pripadao je vrsti Enterococcus faecalis (80%), dok je vrsti Enterococcus faecium pripadao znatno manji procenat izolata (20%). Od ostalih takozvanih streptokokama sličnih vrsta bakterija izolovane su: Aerococcus viridans, Globicatella sanguinis i Lactococcus lactis ssp. cremoris., The aim of this investigation was to establish the presence and prevalence of streptococci, enterococci and streptococci-like bacteria in various materials originating from healthy, slaughtered and dead pigs belonging to different categories from several farms and slaughterhouses in the Republic of Serbia. The total number of investigated samples comprised 226 swabs of tonsils and noses from clinically healthy breeders, swabs of tonsils from piglets 5-20 days old, parts of nasopharyngeal tonsils from breeders slaughtered in a slaughterhouse, parts of nasopharyngeal tonsils from piglets slaughtered in a slaughterhouse, swabs of slaughtered pig carcasses from a slaughterhouse, swabs from knives for evisceration in a slaughterhouse, as well as swabs of lungs, abdominal cavity and organs from piglets which died suddenly. The standard microbiological methods were used for investigations of the presence of the listed microorganisms. Commercial biochemical tests were used for the identification of the isolated bacteria and specific sera for capsular antigenes were used for serological determination of the isolated S. suis strains. It was established that the great majority of the isolated strains belonged to the genus Streptococcus (36) (75%), and the minority of the strains belonged to the following genera: Enterococcus (6) (10.4%), Aerococcus (3) (6.2%), Lactococcus (2) (4.2%) and Globicatella (2) (4.2%). The great majority of Streptococcus species belonged to S. suis. The presence of other á haemolytic streptococci was established in the swabs of nasopharyngeal tonsils: Streptococcus sanguinis (13.8%), Streptococcus salivarius (5.6%), Streptococcus mitis (5.6%), Streptococcus parasanguinis (2.7%) and Streptococcus oralis (2.7%). Also, S. bovis was isolated in a smaller percentage (5.6%). The greatest number of isolated bacteria from the genus Enterococcus belonged to Enterococcus faecalis (80%), while the minority of isolated strains belonged to Enterococcus faecium (20%). The following from the streptococci-like bacteria were isolated: Aerococcus viridans, Globicatella sanguinis and Lactococcus lactis ssp.cremoris.",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs, Ispitivanje prisustva alfa hemolitičkih streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od svinja",
volume = "65",
number = "3-4",
pages = "203-213",
doi = "10.2298/VETGL1104203S"
}

Stanojković, A., Ašanin, R., Ašanin, J., Palić, K., Stanojković, A.,& Žutić, J.. (2011). Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 65(3-4), 203-213.
https://doi.org/10.2298/VETGL1104203S

Stanojković A, Ašanin R, Ašanin J, Palić K, Stanojković A, Žutić J. Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs. in Veterinarski Glasnik. 2011;65(3-4):203-213.
doi:10.2298/VETGL1104203S .

Stanojković, Aleksandar, Ašanin, Ružica, Ašanin, Jelena, Palić, Ksenija, Stanojković, Aleksandra, Žutić, Jadranka, "Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs" in Veterinarski Glasnik, 65, no. 3-4 (2011):203-213,
https://doi.org/10.2298/VETGL1104203S . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Stojanović, Dragica
AU  - Žutić, Milenko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/672
AB  - Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype.
AB  - Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens
T1  - Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena
VL  - 60
IS  - 5-6
SP  - 497
EP  - 506
DO  - 10.2298/AVB1006497Z
ER  - 

@article{
author = "Žutić, Jadranka and Ašanin, Jelena and Mišić, Dušan and Jakić-Dimić, Dobrila and Milić, Nenad and Ašanin, Ružica and Stojanović, Dragica and Žutić, Milenko",
year = "2010",
abstract = "Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype., Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens, Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena",
volume = "60",
number = "5-6",
pages = "497-506",
doi = "10.2298/AVB1006497Z"
}

Žutić, J., Ašanin, J., Mišić, D., Jakić-Dimić, D., Milić, N., Ašanin, R., Stojanović, D.,& Žutić, M.. (2010). Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 497-506.
https://doi.org/10.2298/AVB1006497Z

Žutić J, Ašanin J, Mišić D, Jakić-Dimić D, Milić N, Ašanin R, Stojanović D, Žutić M. Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd. 2010;60(5-6):497-506.
doi:10.2298/AVB1006497Z .

Žutić, Jadranka, Ašanin, Jelena, Mišić, Dušan, Jakić-Dimić, Dobrila, Milić, Nenad, Ašanin, Ružica, Stojanović, Dragica, Žutić, Milenko, "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):497-506,
https://doi.org/10.2298/AVB1006497Z . .

TY  - JOUR
AU  - Ašanin, Ružica
AU  - Žutić, Milenko
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Žutić, Jadranka
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/592
AB  - The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. .
AB  - Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets
T1  - Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi
VL  - 63
IS  - 5-6
SP  - 311
EP  - 320
DO  - 10.2298/VETGL0906311A
ER  - 

@article{
author = "Ašanin, Ružica and Žutić, Milenko and Ašanin, Jelena and Mišić, Dušan and Žutić, Jadranka and Jakić-Dimić, Dobrila and Milić, Nenad and Nišavić, Jakov",
year = "2009",
abstract = "The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. ., Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets, Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi",
volume = "63",
number = "5-6",
pages = "311-320",
doi = "10.2298/VETGL0906311A"
}

Ašanin, R., Žutić, M., Ašanin, J., Mišić, D., Žutić, J., Jakić-Dimić, D., Milić, N.,& Nišavić, J.. (2009). The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 311-320.
https://doi.org/10.2298/VETGL0906311A

Ašanin R, Žutić M, Ašanin J, Mišić D, Žutić J, Jakić-Dimić D, Milić N, Nišavić J. The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik. 2009;63(5-6):311-320.
doi:10.2298/VETGL0906311A .

Ašanin, Ružica, Žutić, Milenko, Ašanin, Jelena, Mišić, Dušan, Žutić, Jadranka, Jakić-Dimić, Dobrila, Milić, Nenad, Nišavić, Jakov, "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets" in Veterinarski Glasnik, 63, no. 5-6 (2009):311-320,
https://doi.org/10.2298/VETGL0906311A . .

TY  - JOUR
AU  - Žutić, Milenko
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Ivetić, V.
AU  - Vidić, Branka
AU  - Žutić, Jadranka
AU  - Ašanin, Jelena
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/555
AB  - The presence of Actinobacillus pleuropneumoniae has been established in all suspected cases of pleuropneumonia at several farms that have been included in the research. Equal incidence of pleuropneumonia has been found both among piglets for breeding and for fattening. The health monitoring of herds is extremely important, firstly because of the need for the adequate strategy to be chosen for controlling the Actinobacillus-caused pleuropneumonia and, at the same time, in order to prevent enormous economic losses that this disease may cause. The morphological, physiological and biochemical characteristics of isolated strains of Actinobacillus pleuropneumoniae were completely identical to those of referent strains. It has been found that Actinobacillus pleuropneumoniae grows most abundantly on chocolate agar when PolyVitex (bioMerieux) is used as the substrate. Out of 237 samples of altered parts of swine lungs, 13 bacterial species have been found in 193 (81%), and the incidence of Actinobacillus pleuropneumoniae within this percentage was 33%. Of all bacterial species isolated in pure culture from all investigated specimen the most dominant species were Pasteurella multocida with the incidence of 32.64% and Actinobacillus pleuropneumoniae with 29.01%. Their common incidence in all positive findings was 61.65%. If one adds to this their participation in mixed infections (2.59%), this percentage is higher (64.24%). The high percentage of these two bacterial species shows that they are at the same time the most common bacterial pathogens causing pneumonia in pigs. The incidence of other species of bacteria isolated from the lungs of diseased pigs ranged from 0.51 to 10.88%. The sensitivity of isolated strains of Actinobacillus pleuropneumoniae to the selected range of antibiotics used in clinical veterinary medicine (penicillin, ampicillin, amoxicillin, cephalosporin (III gen.) gentamycin, streptomycin, neomycin, thylosine, enrofloxacin, linkomycin-spectinomycin, tetracycline, florphenycol, trimethoprimsulphomethoxazol and tulatromycin) was tested by the disc-diffusion method, with the implementation of antibiogram tabletes (Torlak) and antibiogram discs (Oxoid), on chocolate agar and on Chaemophilus test medium (HTM, Biomedics). All tested strains of Actinobacillus pleuropneumoniae were sensitive to thulatromycin, while resistance of same strains was the highest to tetracycline (53%) and trimethoprim/sulphomethoxazole (56%).
AB  - U novije vreme, pleuropneumonija izazvana aktinobacilusom dominira u velikim aglomeracijama životinja i u nekim zemljama predstavlja jedan od problema koji značajno doprinosi smanjenju populacije svinja. Navedeni problem, postoji i na našim farmama svinja gde protiče kao infekcija izazvana jednim uzročnikom, ili se, što je češća pojava, uzročnik javlja kao oportunistična patogena bakterija udružena sa drugim mikroorganizmima koji učestvuju u nastanku kompleksa respiratornih bolesti. Iz navedenih razloga monitoring zdravstvenog stanja jedinki na farmi je neophodan ne samo zbog utvrđivanja odgovarajuće strategije za kontrolu pleuropneumonije koju izaziva aktinobacilus, već i radi sprečavanja ogromnih ekonomskih gubitaka koje ova bolest može da prouzrokuje. U svim slučajevima sumnje na pleuropneumoniju kod svinja sa nekoliko farmi koje su bile obuhvaćene ispitivanjem, ustanovljeno je prisustvo Actinobacillus pleuropneumoniae. Podjednaka zastupljenost pleuropneumonije ustanovljena je, kako kod prasadi u odgoju, tako i kod prasadi u tovu. Od 237 uzoraka promenjenih delova pluća uginulih svinja iz 193 (81%) izolovano je 13 vrsta bakterija, a u okviru navedenog procenta zastupljenost Actinobacillus pleuropneumoniae je iznosila 33,15%. Morfološke, fiziološke i biohemijske osobine izolovanih sojeva Actinobacillus pleuropneumoniae potpuno su bile identične sa osobinama referentnog soja. Utvrđeno je da Actinobacillus pleuropneumoniae najobilnije raste na čokoladnom agaru kada se kao supstrat za obogaćenje koristi PolyVitex (bioMerieux). Od bakterija koje su iz pluća izolovane u čistoj kulturi najzastupljenije su bile Pasteurella multocida sa 32,64% i Actinobacillus pleuropneumoniae sa 29,01%. Njihovo zajedničko učešće u ukupno pozitivnim nalazima iznosi 61,65%, a ako se tome doda i njihovo učešće u mešanim infekcijama od 2,59 % onda taj procenat iznosi 64,24%. Ovako visoki procenti zastupljenosti obe vrste bakterija ukazuju na njihovu značajnu ulogu u nastanku pneumonije kod svinja. Zastupljenost ostalih vrsta bakterija izolovanih iz pluća obolelih svinja kretala se od 0,51% do 10,88%. Kod izolovanih sojeva Actinobacillus pleuropneumoniae osetljivost na odabrani broj antibiotika koji se koriste u kliničkoj praksi (penicilin, ampicilin, amoksicilin, cefalosporine (III gen.), gentamicin, streptomicin, neomicin, tilozin, enrofloksacin, linkomicin-spektinomicin, tetraciklin, florfenikol, trimetoprim-sulfometoksazol i tulatromicin) ispitivana je disk-difuzionom metodom, primenom antibiogram tableta (Torlak) i antibiogram diskova (Oxoid), na čokoladnom agaru i na hemofilus test medijumu (HTM, Biomedics). Svi ispitivani sojevi Actinobacillus pleuropneumoniae bili su osetljivi na tulatromicin, a najveća rezistencija kod istih sojeva, ustanovljena je na trimetoprim/sulfometoksazol od 56% i na tetraciklin od 53%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics
T1  - Izolacija i identifikacija Actinobacillus pleuropneumoniae iz pluća svinja u farmskim uslovima držanja i njihova osetljivost na antibiotike
VL  - 58
IS  - 5-6
SP  - 499
EP  - 507
DO  - 10.2298/AVB0806499Z
ER  - 

@article{
author = "Žutić, Milenko and Ašanin, Ružica and Milić, Nenad and Ivetić, V. and Vidić, Branka and Žutić, Jadranka and Ašanin, Jelena",
year = "2008",
abstract = "The presence of Actinobacillus pleuropneumoniae has been established in all suspected cases of pleuropneumonia at several farms that have been included in the research. Equal incidence of pleuropneumonia has been found both among piglets for breeding and for fattening. The health monitoring of herds is extremely important, firstly because of the need for the adequate strategy to be chosen for controlling the Actinobacillus-caused pleuropneumonia and, at the same time, in order to prevent enormous economic losses that this disease may cause. The morphological, physiological and biochemical characteristics of isolated strains of Actinobacillus pleuropneumoniae were completely identical to those of referent strains. It has been found that Actinobacillus pleuropneumoniae grows most abundantly on chocolate agar when PolyVitex (bioMerieux) is used as the substrate. Out of 237 samples of altered parts of swine lungs, 13 bacterial species have been found in 193 (81%), and the incidence of Actinobacillus pleuropneumoniae within this percentage was 33%. Of all bacterial species isolated in pure culture from all investigated specimen the most dominant species were Pasteurella multocida with the incidence of 32.64% and Actinobacillus pleuropneumoniae with 29.01%. Their common incidence in all positive findings was 61.65%. If one adds to this their participation in mixed infections (2.59%), this percentage is higher (64.24%). The high percentage of these two bacterial species shows that they are at the same time the most common bacterial pathogens causing pneumonia in pigs. The incidence of other species of bacteria isolated from the lungs of diseased pigs ranged from 0.51 to 10.88%. The sensitivity of isolated strains of Actinobacillus pleuropneumoniae to the selected range of antibiotics used in clinical veterinary medicine (penicillin, ampicillin, amoxicillin, cephalosporin (III gen.) gentamycin, streptomycin, neomycin, thylosine, enrofloxacin, linkomycin-spectinomycin, tetracycline, florphenycol, trimethoprimsulphomethoxazol and tulatromycin) was tested by the disc-diffusion method, with the implementation of antibiogram tabletes (Torlak) and antibiogram discs (Oxoid), on chocolate agar and on Chaemophilus test medium (HTM, Biomedics). All tested strains of Actinobacillus pleuropneumoniae were sensitive to thulatromycin, while resistance of same strains was the highest to tetracycline (53%) and trimethoprim/sulphomethoxazole (56%)., U novije vreme, pleuropneumonija izazvana aktinobacilusom dominira u velikim aglomeracijama životinja i u nekim zemljama predstavlja jedan od problema koji značajno doprinosi smanjenju populacije svinja. Navedeni problem, postoji i na našim farmama svinja gde protiče kao infekcija izazvana jednim uzročnikom, ili se, što je češća pojava, uzročnik javlja kao oportunistična patogena bakterija udružena sa drugim mikroorganizmima koji učestvuju u nastanku kompleksa respiratornih bolesti. Iz navedenih razloga monitoring zdravstvenog stanja jedinki na farmi je neophodan ne samo zbog utvrđivanja odgovarajuće strategije za kontrolu pleuropneumonije koju izaziva aktinobacilus, već i radi sprečavanja ogromnih ekonomskih gubitaka koje ova bolest može da prouzrokuje. U svim slučajevima sumnje na pleuropneumoniju kod svinja sa nekoliko farmi koje su bile obuhvaćene ispitivanjem, ustanovljeno je prisustvo Actinobacillus pleuropneumoniae. Podjednaka zastupljenost pleuropneumonije ustanovljena je, kako kod prasadi u odgoju, tako i kod prasadi u tovu. Od 237 uzoraka promenjenih delova pluća uginulih svinja iz 193 (81%) izolovano je 13 vrsta bakterija, a u okviru navedenog procenta zastupljenost Actinobacillus pleuropneumoniae je iznosila 33,15%. Morfološke, fiziološke i biohemijske osobine izolovanih sojeva Actinobacillus pleuropneumoniae potpuno su bile identične sa osobinama referentnog soja. Utvrđeno je da Actinobacillus pleuropneumoniae najobilnije raste na čokoladnom agaru kada se kao supstrat za obogaćenje koristi PolyVitex (bioMerieux). Od bakterija koje su iz pluća izolovane u čistoj kulturi najzastupljenije su bile Pasteurella multocida sa 32,64% i Actinobacillus pleuropneumoniae sa 29,01%. Njihovo zajedničko učešće u ukupno pozitivnim nalazima iznosi 61,65%, a ako se tome doda i njihovo učešće u mešanim infekcijama od 2,59 % onda taj procenat iznosi 64,24%. Ovako visoki procenti zastupljenosti obe vrste bakterija ukazuju na njihovu značajnu ulogu u nastanku pneumonije kod svinja. Zastupljenost ostalih vrsta bakterija izolovanih iz pluća obolelih svinja kretala se od 0,51% do 10,88%. Kod izolovanih sojeva Actinobacillus pleuropneumoniae osetljivost na odabrani broj antibiotika koji se koriste u kliničkoj praksi (penicilin, ampicilin, amoksicilin, cefalosporine (III gen.), gentamicin, streptomicin, neomicin, tilozin, enrofloksacin, linkomicin-spektinomicin, tetraciklin, florfenikol, trimetoprim-sulfometoksazol i tulatromicin) ispitivana je disk-difuzionom metodom, primenom antibiogram tableta (Torlak) i antibiogram diskova (Oxoid), na čokoladnom agaru i na hemofilus test medijumu (HTM, Biomedics). Svi ispitivani sojevi Actinobacillus pleuropneumoniae bili su osetljivi na tulatromicin, a najveća rezistencija kod istih sojeva, ustanovljena je na trimetoprim/sulfometoksazol od 56% i na tetraciklin od 53%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics, Izolacija i identifikacija Actinobacillus pleuropneumoniae iz pluća svinja u farmskim uslovima držanja i njihova osetljivost na antibiotike",
volume = "58",
number = "5-6",
pages = "499-507",
doi = "10.2298/AVB0806499Z"
}

Žutić, M., Ašanin, R., Milić, N., Ivetić, V., Vidić, B., Žutić, J.,& Ašanin, J.. (2008). Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 499-507.
https://doi.org/10.2298/AVB0806499Z

Žutić M, Ašanin R, Milić N, Ivetić V, Vidić B, Žutić J, Ašanin J. Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics. in Acta Veterinaria-Beograd. 2008;58(5-6):499-507.
doi:10.2298/AVB0806499Z .

Žutić, Milenko, Ašanin, Ružica, Milić, Nenad, Ivetić, V., Vidić, Branka, Žutić, Jadranka, Ašanin, Jelena, "Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):499-507,
https://doi.org/10.2298/AVB0806499Z . .