TY  - JOUR
AU  - Tešović, Bojana
AU  - Nišavić, Jakov
AU  - Banović-Đeri, Bojana
AU  - Petrović, Tamaš
AU  - Radalj, Andrea
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Debeljak, Zoran
AU  - Vasković, Nikola
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2520
AB  - West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.
PB  - Elsevier
T2  - Diagnostic Microbiology and Infectious Disease
T1  - Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform
VL  - 105
IS  - 2
SP  - 115852
DO  - 10.1016/j.diagmicrobio.2022.115852
ER  - 

@article{
author = "Tešović, Bojana and Nišavić, Jakov and Banović-Đeri, Bojana and Petrović, Tamaš and Radalj, Andrea and Šekler, Milanko and Matović, Kazimir and Debeljak, Zoran and Vasković, Nikola and Dmitrić, Marko and Vidanović, Dejan",
year = "2023",
abstract = "West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.",
publisher = "Elsevier",
journal = "Diagnostic Microbiology and Infectious Disease",
title = "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform",
volume = "105",
number = "2",
pages = "115852",
doi = "10.1016/j.diagmicrobio.2022.115852"
}

Tešović, B., Nišavić, J., Banović-Đeri, B., Petrović, T., Radalj, A., Šekler, M., Matović, K., Debeljak, Z., Vasković, N., Dmitrić, M.,& Vidanović, D.. (2023). Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease
Elsevier., 105(2), 115852.
https://doi.org/10.1016/j.diagmicrobio.2022.115852

Tešović B, Nišavić J, Banović-Đeri B, Petrović T, Radalj A, Šekler M, Matović K, Debeljak Z, Vasković N, Dmitrić M, Vidanović D. Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform. in Diagnostic Microbiology and Infectious Disease. 2023;105(2):115852.
doi:10.1016/j.diagmicrobio.2022.115852 .

Tešović, Bojana, Nišavić, Jakov, Banović-Đeri, Bojana, Petrović, Tamaš, Radalj, Andrea, Šekler, Milanko, Matović, Kazimir, Debeljak, Zoran, Vasković, Nikola, Dmitrić, Marko, Vidanović, Dejan, "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform" in Diagnostic Microbiology and Infectious Disease, 105, no. 2 (2023):115852,
https://doi.org/10.1016/j.diagmicrobio.2022.115852 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Debeljak, Mihailo
AU  - Stojanović, Milovan
AU  - Glavčić, Marko
AU  - Marinković, Darko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3340
AB  - Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp.
AB  - Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Prikaz slučaja leptospiroze kod dve alpake
T1  - Leptospirosis in two alpacas - case report
SP  - 134
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3340
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Debeljak, Mihailo and Stojanović, Milovan and Glavčić, Marko and Marinković, Darko",
year = "2023",
abstract = "Лептоспироза је инфективна болест домаћих и дивљих животиња као и човека
коју изазивају патогене спирохете из рода Leptospira. Овом роду припада 66
врста и преко 300 серовара, разврстаних у 30 серогрупа. Болест се јавља на свим
континентима изузев Антарктика, а манифестује се широким спектром
клиничких симптома, међу којима су грозница, повраћање, дијареја, губитак
апетита, иктерус, хемоглобинурија, крвављења по кожи и слузницама, увеитис
и побачаји.
У лабораторију ВСИ Краљево из приватног зоолошког врта допремљена су два
леша алпаке (Vicugna pacos), мужјак стар 4 године и женка стара годину дана.
Заживотно, животиње су испољавале опште и неуролошке симптоме и боловале
су свега неколико сати. Обдукцијом су утврђене макроскопске промене:
асцитес, хидроперикард, субепикардијална крвављења, хиперемија и едем
плућа, крвављења на слезини, нефритис и хеморагични ентеритис.
Хистопатолошким прегледом на бубрезима су утврђен мултифокални
интерстицијални нефритис и тубулонекроза хиперемија и глиоза мозгу, некрозе
и крвављења на јетри, на слезини крвављења и фоликуларни спленитис, док је
на цревима регистрован некротични ентеритис.
У узорцима органа бактериолошком изолацијом и идентификацијом је доказано
присуство Clostridium perfringens. С обзиром да је патоморфолошким прегледом
постављена сумња на лептоспирозу, а да је бактериолошка изолација
комплексна, методом real-time PCR у бубрезима угинулих алпака доказано је
присуство генома патогених сојева Leptospira spp., Leptospirosis is an infectious disease of domestic and wild animals and humans
caused by pathogenic spirochetes of the Leptospira genus. This genus includes 66
species and over 300 serovars, classified into 30 serogroups. The disease occurs in all
continents except Antarctica and is manifested by a wide range of clinical symptoms,
including fever, vomiting, diarrhea, loss of appetite, icterus, hemoglobinuria,
hemorrhages of the skin and mucous membranes, uveitis and abortions.
Two carcasses of alpaca (Vicugna pacos), a 4-year-old male and a one-year-old
female, were delivered to the laboratory of VSI Kraljevo from the private zoo. Just
few hours before death animals showed general and neurological symptoms.
Macroscopic changes established by necropsy were: ascites, hydropericardium,
subepicardial hemorrhages, lung hyperemia and edema, spleen hemorrhages,
nephritis, and hemorrhagic enteritis. Histopathological examination revealed
interstitial nephritis and tubulonecrosis on kidneys, hyperemia and gliosis in the brain,
necrosis and hemorrhages in the liver, follicular splenitis, while necrotic enteritis was
noted on the intestine.
The presence of Clostridium perfringens in organ samples was proven by
bacteriological isolation and identification. Based on pathomorphological suspicion
on leptospirosis and due to complex bacteriological isolation, the presence of
pathogenic strains of Leptospira spp. genome in the kidneys of dead alpacas was
proven by the real time-PCR method.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Prikaz slučaja leptospiroze kod dve alpake, Leptospirosis in two alpacas - case report",
pages = "134-135",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3340"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Debeljak, M., Stojanović, M., Glavčić, M.,& Marinković, D.. (2023). Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Debeljak M, Stojanović M, Glavčić M, Marinković D. Prikaz slučaja leptospiroze kod dve alpake. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:134-135.
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Debeljak, Mihailo, Stojanović, Milovan, Glavčić, Marko, Marinković, Darko, "Prikaz slučaja leptospiroze kod dve alpake" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):134-135,
https://hdl.handle.net/21.15107/rcub_veterinar_3340 .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3175
AB  - Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести.
AB  - Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
T1  - Prikaz slučaja Aujeskijeve bolesti kod lovnog psa
T1  - Aujezsky’s disease in a hunting dog - case report
SP  - 52
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3175
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2022",
abstract = "Аујескијева болест или лажно беснило је акутна, високо контагиозна болест многих врста сисара. Узрочник је свињски Alphaherpesvirus 1, ДНК вирус који припада роду Varicellovirus, фамилији Herpesviridae. Свиње су главни домаћин и резервоар вируса, и код њих се болест обично карактерише неуролошким, респираторним симптомима и абортусом. Код млађих категорија морталитет може бити веома висок, док одрасле свиње углавном имају благе симптоме и опораве се. Код говеда, оваца и месоједа болест је неизлечива и карактерише се неуролошким симптомима и јаким сврабом.
У лабораторију ВСИ Краљево допремљена је глава пса под сумњом на Аујескијеву болест. Пас који је током лова био у директном контакту са дивљом свињом боловао је два дана са следећим симптомима: безвољност, губитак апетита, отежано кретање, појачана саливација, оток лица и изражени свраб. Након отварања лобањске дупље, уочена је препуњеност крвних судова можданих овојница, а делови мозга су узорковани за лабораторијске анализе. Методом директе имунофлуоресценције добијен је негативни резултат на присуство вируса беснила. Установљене су хистопатолошке промене у форми негнојног енцефалитиса који се карактерисао периваскуларним инфилтратом мононуклеарним ћелија, глиозом, дегенерацијом неурона, сателитозом, неуронофагијом и крвављењем. Методом real time-PCR у мозгу угинулог пса доказано је присуство генома узрочника Аујескијеве болести., Aujeszky's disease or pseudorabies is an acute, highly contagious disease of many mammalian species. The causative agent is suid alphaherpesvirus 1, a DNA virus which belongs to the genus Varicellovirus, family Herpesviridae. Pigs are the main hosts and reservoirs of the virus, and their disease is usually characterized by neurological, respiratory symptoms and abortion. In younger categories, mortality can be very high, while adult pigs usually have mild symptoms and recover after infection. In cattle, sheep and carnivores, the disease is incurable and is characterized by neurological symptoms and severe itching.
Head of a dog suspected of Aujeszky's disease was delivered to the VSI Kraljevo laboratory. The dog was in direct contact with a wild boar during the hunt and showed following symptoms for two days: lethargy, loss of appetite, muscle stiffness, hypersalivation, severe itching and face swelling. After opening the cranial cavity, a meningeal hyperaemia was noticed, and parts of the brain were sampled for laboratory analyses. The method of direct immunofluorescence gave a negative result on the presence of rabies virus. Histopathological lesions in the form of non-purulent encephalitis have been noticed and was characterised by perivascular infiltrates of mononuclear cells, gliosis, neuronal degeneration, satellitosis, neuronophagy and hemorrhage. The presence of the Aujeszky's disease virus genome in the brain of the dog has been proven by real-time PCR.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022",
title = "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa, Aujezsky’s disease in a hunting dog - case report",
pages = "52-53",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3175"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2022). Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja Aujeskijeve bolesti kod lovnog psa. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022. 2022;:52-53.
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja Aujeskijeve bolesti kod lovnog psa" in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022 (2022):52-53,
https://hdl.handle.net/21.15107/rcub_veterinar_3175 .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2926
AB  - Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса.
AB  - Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
T1  - Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji
T1  - Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2926
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Tešović, Bojana and Aničić, Milan and Marinković, Darko",
year = "2021",
abstract = "Нодуларни дерматитис је вирусна болест говеда и домаћих бивола која може да доведе до значајних економских губитака. Узрочник припада роду Capripoxvirus, фамилији Poxviridae, а најбитнију улогу у ширењу болести имају хематофагни артроподи, као биолошки и механички вектори. Иако је болест је дуго била ограничена на Азију и Блиски Исток, током 2015. и 2016. године проширила се на југоисточну Европу, укључујући и Србију.
Макроскопске промене на кожи говеда инфицираних вирусом нодуларног дерматитиса манифестовале су се у форми чворова који су били округли, уздигнути, јасно ограничени, чврсти и насумично распоређени по целој кожи, укључујући стидницу и виме. Хистопатолошке промене су биле у форми дегенерације епителијалних ћелија, хиперкератозе, спонгиозе и акантозе, присутне у 85,88%, 81.18%, 84,71%, и 80.0% узорака. Интрацитоплазматске инклузије су примећене у кератиноцитима stratum spinosum -а у 56,47% узорака, а у ћелијама фоликула длаке и епителним ћелијама лојних жлезда у 45,88% узорака. Дермалне промене биле су у облику запаљенског инфилтрата присутног у дермису и субкутису у 97,65% узорака.
Због карактеристичних морфолошких промена коју узрочник изазива у кожи оболелих животиња, хистопатологија представља врло корисну методу за дијагнозу нодуларног дерматитиса., Lumpy skin disease is a viral disease of cattle and domestic buffaloes which can lead to significant economic losses. The causative agent belongs to the genus Capripoxvirus, the family Poxviridae, and the most important role in the spread of the disease is played by hematophagous arthropods, as biological and mechanical vectors. Although the disease has been restricted to Asia and the Middle East for many years, in 2015 and 2016 it was spread to Southeastern Europe, including Serbia.
Gross lesions in the skin of the cattle infected with Lumpy skin disease virus were manifested in a form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Dermal lesions were in form of inflammatory infiltrate present in dermis and subcutis in 97.65% of samples.
Due to the characteristic morphological lesions caused by the pathogen in the skin of diseased animals, histopathology should be considered as a very useful method for the diagnosis of Lumpy Skin Disease.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021",
title = "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji, Pathomorphological lesions of skin in cattle naturally infected with lumpy skin disease virus in Serbia",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2926"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D., Tešović, B., Aničić, M.,& Marinković, D.. (2021). Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Tešović B, Aničić M, Marinković D. Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji. in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021. 2021;:140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Tešović, Bojana, Aničić, Milan, Marinković, Darko, "Patomorfološke promene u koži goveda prirodno inficiranih virusom nodularnog dermatitisa u Srbiji" in XXII / XXIII Simpozijum epizootiologa i epidemiologa / XXII / XXIII Epizootiološki dani, Beograd, 26 - 28. april, 2021 (2021):140-141,
https://hdl.handle.net/21.15107/rcub_veterinar_2926 .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Radojičić, Sonja
AU  - Debeljak, Zoran
AU  - Šekler, Milanko
AU  - Ćirić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797
AB  - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
PB  - Elsevier, Amsterdam
T2  - Saudi Journal of Biological Sciences
T1  - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
VL  - 27
IS  - 1
SP  - 518
EP  - 523
DO  - 10.1016/j.sjbs.2019.11.012
ER  - 

@article{
author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena",
year = "2020",
abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Journal of Biological Sciences",
title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia",
volume = "27",
number = "1",
pages = "518-523",
doi = "10.1016/j.sjbs.2019.11.012"
}

Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences
Elsevier, Amsterdam., 27(1), 518-523.
https://doi.org/10.1016/j.sjbs.2019.11.012

Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523.
doi:10.1016/j.sjbs.2019.11.012 .

Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523,
https://doi.org/10.1016/j.sjbs.2019.11.012 . .

TY  - JOUR
AU  - Imatdinov, Almaz R.
AU  - Kazakova, Anna S.
AU  - Šekler, Milanko
AU  - Morozova, Diana Yu
AU  - Lyska, Valentina M.
AU  - Titov, Ilya A.
AU  - Sevskikh, Timofey
AU  - Sereda, Alexey D.
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2407
AB  - Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated.
AB  - Kao potencijalni induktori specifičnog antivirusnog celularnog imuniteta, kreirane su 3 rekombinantne plazmidne konstrukcije, koje predstavljaju himerne proteine, koje sadrže humani ubikvitin, na čijem ektodomenu je inkorporiran po jedan od sledećih potencijalno zaštićenih proteina (p30, p54 i CD2v), a koji potiču od atenuiranog soja afričke kuge svinja MK - 200. Navedenim plazmidima je izvršena trostruka imunizacija svinja, koja je kod njih dovela do posledičnog formiranja virus specifičnih citotoksičnih T limfocita (CTL), ali bez uticaja na indukciju sinteze virus specifičnih antitela. Nakon veštačke infekcije ovih imunizovanih svinja i to homolognim virulentnim sojem virusa afričke kuge svinja M - 78, u dozi od 103  HAD50, svih 5 svinja (4 imunizovane svinje i 1 neimunizovana - kontrolna) su uginule između 4 i 7 dana nakon infi ciranja. Dobijeni rezultati su pokazali da indukcija samo celularnog imunog odgovora, odnosno citotoksičnih T limfocita, ne štiti svinje od infekcije virulentnim sojem virusa afričke kuge svinja. Izbalansiranu aktivaciju citotoksičnih T limfocita i antitelima posredovane ćelijske imunske mehanizme trebalo bi dalje istražiti.
T2  - Acta Veterinaria-Beograd
T1  - Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
T1  - Imunizacija svinja sa rekombinantnim plazmidima koji sadrže gene ubikvitarnih p30, p54 i cd2v proteina virusa afričke kuge svinja
VL  - 70
IS  - 1
SP  - 92
EP  - 109
DO  - 10.2478/acve-2020-0007
ER  - 

@article{
author = "Imatdinov, Almaz R. and Kazakova, Anna S. and Šekler, Milanko and Morozova, Diana Yu and Lyska, Valentina M. and Titov, Ilya A. and Sevskikh, Timofey and Sereda, Alexey D.",
year = "2020",
abstract = "Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated., Kao potencijalni induktori specifičnog antivirusnog celularnog imuniteta, kreirane su 3 rekombinantne plazmidne konstrukcije, koje predstavljaju himerne proteine, koje sadrže humani ubikvitin, na čijem ektodomenu je inkorporiran po jedan od sledećih potencijalno zaštićenih proteina (p30, p54 i CD2v), a koji potiču od atenuiranog soja afričke kuge svinja MK - 200. Navedenim plazmidima je izvršena trostruka imunizacija svinja, koja je kod njih dovela do posledičnog formiranja virus specifičnih citotoksičnih T limfocita (CTL), ali bez uticaja na indukciju sinteze virus specifičnih antitela. Nakon veštačke infekcije ovih imunizovanih svinja i to homolognim virulentnim sojem virusa afričke kuge svinja M - 78, u dozi od 103  HAD50, svih 5 svinja (4 imunizovane svinje i 1 neimunizovana - kontrolna) su uginule između 4 i 7 dana nakon infi ciranja. Dobijeni rezultati su pokazali da indukcija samo celularnog imunog odgovora, odnosno citotoksičnih T limfocita, ne štiti svinje od infekcije virulentnim sojem virusa afričke kuge svinja. Izbalansiranu aktivaciju citotoksičnih T limfocita i antitelima posredovane ćelijske imunske mehanizme trebalo bi dalje istražiti.",
journal = "Acta Veterinaria-Beograd",
title = "Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus, Imunizacija svinja sa rekombinantnim plazmidima koji sadrže gene ubikvitarnih p30, p54 i cd2v proteina virusa afričke kuge svinja",
volume = "70",
number = "1",
pages = "92-109",
doi = "10.2478/acve-2020-0007"
}

Imatdinov, A. R., Kazakova, A. S., Šekler, M., Morozova, D. Y., Lyska, V. M., Titov, I. A., Sevskikh, T.,& Sereda, A. D.. (2020). Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus. in Acta Veterinaria-Beograd, 70(1), 92-109.
https://doi.org/10.2478/acve-2020-0007

Imatdinov AR, Kazakova AS, Šekler M, Morozova DY, Lyska VM, Titov IA, Sevskikh T, Sereda AD. Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus. in Acta Veterinaria-Beograd. 2020;70(1):92-109.
doi:10.2478/acve-2020-0007 .

Imatdinov, Almaz R., Kazakova, Anna S., Šekler, Milanko, Morozova, Diana Yu, Lyska, Valentina M., Titov, Ilya A., Sevskikh, Timofey, Sereda, Alexey D., "Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus" in Acta Veterinaria-Beograd, 70, no. 1 (2020):92-109,
https://doi.org/10.2478/acve-2020-0007 . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Tomić, Aleksandar
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3337
AB  - У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима.
AB  - In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Prikaz slučaja mononuklearnog dermatitisa fazana
T1  - Case report: Mononuclear dermatitis in pheasant
SP  - 164
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3337
ER  - 

@conference{
author = "Vasković, Nikola and Debeljak, Zoran and Tomić, Aleksandar and Matović, Kazimir and Šekler, Milanko and Vidanović, Dejan and Marinković, Darko",
year = "2019",
abstract = "У новембру 2018. године, у лабораторију ВСИ Краљево достављен је леш
фазана код кога су ловци приметили неуобичајене промене на глави. Птица је
одстрељена током редовног лова, била је мушког пола, полно зрела, добре
кондиције и стања ухрањености.
Патоанатомским прегледом утврђене су трауме настале дејством ватреног
оружја и уједне ране. На кожи главе, у периокуларној, периназалној регији и на
подбрадњацима, биле су присутне брадавичасте израслине бело-жуте боје,
промера 2-5 mm, на пресеку сланинастог изгледа. На врховима неких од њих
била је присутна некроза. Ове израслине су биле обостране, готово симетричне,
а биле су груписане тако да формирају гроздове.
Патохистолошким прегледом ткивних исечака промењених делова коже
обојених стандардном хематоксилин-еозин методом, утврђено је присуство
запаљенског инфилтрата који се састојао из мононуклеарних ћелија, углавном
зрелих лимфоцита, a уочено је присуство и плазмоцита и макрофага. Запажена
је дегенерација и вакуолизација епидермиса, у неким деловима и хиперкератоза.
У недавно објављеном истраживању које је група аутора спровела на 258 лешева
фазана одстрељених у ловиштима на северозападу Немачке, код 62.3 % узорака
описане су промене у типу мононуклеарног дерматитиса, по дистрибуцији и
карактеру инфилтрата врло сличне онима које смо ми установили. Аутори
наводе да се ради о периокуларном и периназалном дерматитису непознате
етиологије. Молекуларним испитивањима промењених делова коже, у једном
узорку утврдили су присуство генома који је врло сличан геному вируса
ретикулоендотелиозе. Међутим, патогени ефекат вируса није утврђен након
инокулације и умножавања на пилећим ембрионима., In November 2018, a pheasant with unusual head lesions was delivered to the
laboratory of the VSI Kraljevo for examination. The bird was shot during the regular
hunting, it was adult male in a good body condition.
Traumas caused by the firearm and bite wounds were identified by the gross
examination. On the skin of the head, in the periocular, perinasal region, and on the
wattles, there were papules of white-yellow colour, 2-5 mm in diameter, bacon-like
appearance on a cross-section. Some of them had necrosis at the tops. These growths
were bilateral, almost symmetrical, and were grouped to form clusters.
Sections of the changed skin were coloured by standard haematoxylin-eosin method.
Microscopically examination revealed the presence of inflammatory infiltrates
consisting of mononuclear cells, mostly mature lymphocytes. Plasma cells and
macrophages were also observed. Degeneration and vacuolisation of the epidermis, in
some areas with hyperkeratosis, were observed.
In a recent study on 258 corpses of pheasants shot in hunting grounds in north-western
Germany, in 62.3% of the samples authors described changes in the type of
mononuclear dermatitis, very similar to those that we established. The authors
suggested that this is a periocular and perinasal dermatitis of unknown aetiology.
Molecular examination of the changed parts of the skin, in one sample, determined
the presence of a genome that was very similar to the genome of the
reticuloendotheliosis virus. However, the pathogenic effect of the virus has not been
established on chicken embryos after inoculation and multiplication.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Prikaz slučaja mononuklearnog dermatitisa fazana, Case report: Mononuclear dermatitis in pheasant",
pages = "164-165",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3337"
}

Vasković, N., Debeljak, Z., Tomić, A., Matović, K., Šekler, M., Vidanović, D.,& Marinković, D.. (2019). Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337

Vasković N, Debeljak Z, Tomić A, Matović K, Šekler M, Vidanović D, Marinković D. Prikaz slučaja mononuklearnog dermatitisa fazana. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:164-165.
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

Vasković, Nikola, Debeljak, Zoran, Tomić, Aleksandar, Matović, Kazimir, Šekler, Milanko, Vidanović, Dejan, Marinković, Darko, "Prikaz slučaja mononuklearnog dermatitisa fazana" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):164-165,
https://hdl.handle.net/21.15107/rcub_veterinar_3337 .

TY  - JOUR
AU  - Vasković, Nikola
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Matović, Kazimir
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1697
AB  - Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia
VL  - 69
IS  - 4
SP  - 369
EP  - 378
DO  - 10.2478/acve-2019-0031
ER  - 

@article{
author = "Vasković, Nikola and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Matović, Kazimir and Aničić, Milan and Marinković, Darko",
year = "2019",
abstract = "Lumpy skin disease (LSD) is a disease of cattle and domestic buffaloes caused by Capripoxvirus which can lead to significant economic losses. Until several years ago it was limited to Africa and the Middle East, but recently it was reported in Turkey (2013), Greece (2015), and in 2016 it spread to Bulgaria, North Macedonia, Albania, Montenegro and Serbia. The aim of this study was to determine the microscopic lesions in the skin of naturally infected animals, highlight their features and compare them to the findings of other authors. Gross lesions in the skin of cattle infected with Lumpy skin disease virus (LSDV) were manifested in the form of skin nodules which were round, raised, clearly circumscribed, firm and randomly distributed in the whole skin including the vulva and udder. Histopathological lesions included degeneration of epithelial cells, hyperkeratosis, spongiosis, and acanthosis present in 85.88%, 81.18%, 84.71%, and 80.0% of samples, respectively. Intracytoplasmic inclusion bodies were noted in keratinocytes of the stratum spinosum in 56.47% of samples, and in hair follicle cells and sebaceous glands epithelial cells in 45.88% of samples. Cutaneous lesions were in the form of inflammatory infiltrate present in the dermis and subcutis in 97.65% of samples. Since there are only a few literature data in this field, this study expands the knowledge relating to morphological alterations in LSD. Based on the characteristic microscopic lesions described in the present study, histopathology should be considered as a very useful method for the diagnosis of LSD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia",
volume = "69",
number = "4",
pages = "369-378",
doi = "10.2478/acve-2019-0031"
}

Vasković, N., Debeljak, Z., Vidanović, D., Šekler, M., Matović, K., Aničić, M.,& Marinković, D.. (2019). Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 369-378.
https://doi.org/10.2478/acve-2019-0031

Vasković N, Debeljak Z, Vidanović D, Šekler M, Matović K, Aničić M, Marinković D. Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia. in Acta Veterinaria-Beograd. 2019;69(4):369-378.
doi:10.2478/acve-2019-0031 .

Vasković, Nikola, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Matović, Kazimir, Aničić, Milan, Marinković, Darko, "Morphological characteristics of skin lesions in cattle naturally infected with lumpy skin disease virus in Serbia" in Acta Veterinaria-Beograd, 69, no. 4 (2019):369-378,
https://doi.org/10.2478/acve-2019-0031 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Kureljušić, Jasna
AU  - Šekler, Milanko
AU  - Nešić, Ksenija
AU  - Veljović, Ljubiša
AU  - Zorić-Maksimović, Jelena
AU  - Radosavljević, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1657
AB  - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.
PB  - Springeropen, London
T2  - AMB EXPRESS
T1  - Molecular detection of black queen cell virus and Kashmir bee virus in honey
VL  - 8
SP  - 128
DO  - 10.1186/s13568-018-0655-7
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Zorić-Maksimović, Jelena and Radosavljević, Vladimir",
year = "2018",
abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.",
publisher = "Springeropen, London",
journal = "AMB EXPRESS",
title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey",
volume = "8",
pages = "128",
doi = "10.1186/s13568-018-0655-7"
}

Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Zorić-Maksimović, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS
Springeropen, London., 8, 128.
https://doi.org/10.1186/s13568-018-0655-7

Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Zorić-Maksimović J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS. 2018;8:128.
doi:10.1186/s13568-018-0655-7 .

Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Zorić-Maksimović, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB EXPRESS, 8 (2018):128,
https://doi.org/10.1186/s13568-018-0655-7 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1581
AB  - After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.
PB  - Allen Press Inc, Lawrence
T2  - Journal of Parasitology
T1  - Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia
VL  - 104
IS  - 5
SP  - 557
EP  - 559
DO  - 10.1645/18-42
ER  - 

@article{
author = "Dmitrić, Marko and Debeljak, Zoran and Vidanović, Dejan and Šekler, Milanko and Vasković, Nikola and Matović, Kazimir and Karabasil, Nedjeljko",
year = "2018",
abstract = "After a human trichinellosis outbreak in Zlatibor District, Serbia, in 2016, Trichinella larvae were found in wild boar (Sus scrofa) meat products. One hundred and fourteen people were infected during the outbreak. The larvae were determined to be Trichinella britovi using the polymerase chain reaction method. Trichinella britovi has previously been identified in Serbia, but this is the first case of the species being confirmed in food samples linked to human trichinellosis. The results of the study confirmed that the T. britovi is able to affect human health. In addition, this study suggests the role of wild boars as reservoirs of T. britovi in Serbia.",
publisher = "Allen Press Inc, Lawrence",
journal = "Journal of Parasitology",
title = "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia",
volume = "104",
number = "5",
pages = "557-559",
doi = "10.1645/18-42"
}

Dmitrić, M., Debeljak, Z., Vidanović, D., Šekler, M., Vasković, N., Matović, K.,& Karabasil, N.. (2018). Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology
Allen Press Inc, Lawrence., 104(5), 557-559.
https://doi.org/10.1645/18-42

Dmitrić M, Debeljak Z, Vidanović D, Šekler M, Vasković N, Matović K, Karabasil N. Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia. in Journal of Parasitology. 2018;104(5):557-559.
doi:10.1645/18-42 .

Dmitrić, Marko, Debeljak, Zoran, Vidanović, Dejan, Šekler, Milanko, Vasković, Nikola, Matović, Kazimir, Karabasil, Nedjeljko, "Trichinella britovi in Game Meat Linked to Human Trichinellosis Outbreak in Serbia" in Journal of Parasitology, 104, no. 5 (2018):557-559,
https://doi.org/10.1645/18-42 . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 

@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}

Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439

Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .

Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Sarić, Ljubiša
AU  - Arsić, Milos
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1641
AB  - The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.
PB  - Wiley, Hoboken
T2  - Journal of Food Processing and Preservation
T1  - In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food
VL  - 42
IS  - 2
SP  - e13455
DO  - 10.1111/jfpp.13455
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Šekler, Milanko and Sarić, Ljubiša and Arsić, Milos and Karabasil, Nedjeljko",
year = "2018",
abstract = "The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Processing and Preservation",
title = "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food",
volume = "42",
number = "2",
pages = "e13455",
doi = "10.1111/jfpp.13455"
}

Dmitrić, M., Vidanović, D., Matović, K., Šekler, M., Sarić, L., Arsić, M.,& Karabasil, N.. (2018). In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation
Wiley, Hoboken., 42(2), e13455.
https://doi.org/10.1111/jfpp.13455

Dmitrić M, Vidanović D, Matović K, Šekler M, Sarić L, Arsić M, Karabasil N. In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation. 2018;42(2):e13455.
doi:10.1111/jfpp.13455 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Šekler, Milanko, Sarić, Ljubiša, Arsić, Milos, Karabasil, Nedjeljko, "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food" in Journal of Food Processing and Preservation, 42, no. 2 (2018):e13455,
https://doi.org/10.1111/jfpp.13455 . .

TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Vasković, Nikola
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Debeljak, Zoran
AU  - Karabasil, Nedjeljko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1483
AB  - Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.
PB  - Amer Assoc Zoo Veterinarians, Yulee
T2  - Journal of Zoo and Wildlife Medicine
T1  - Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia
VL  - 48
IS  - 3
SP  - 703
EP  - 707
DO  - 10.1638/2016-0169.1
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Vasković, Nikola and Matović, Kazimir and Šekler, Milanko and Debeljak, Zoran and Karabasil, Nedjeljko",
year = "2017",
abstract = "Wild animals, including red foxes (Vulpes vulpes) and golden jackals (Canis aureus), are the most important reservoirs of Trichinella spp. Although the red fox is considered one of the main reservoirs of Trichinella spp. in Europe, only a few animals have been examined in Serbia. The present study assessed Trichinella spp. infection in red foxes and golden jackals from the six districts in Serbia. Thirty-seven carcasses of red foxes and 13 carcasses of golden jackals shot during the official hunting season were examined. Larvae of Trichinella spp. were detected in 13 (35%) of 37 red foxes and in 8 (61%) of 13 golden jackals. Trichinella spiralis and Trichinella britovi were the only two species identified after a multiplex polymerase chain reaction analysis. Trichinella britovi infection was detected in 85% of red foxes and in 38% of golden jackals, and T. spiralis was detected in 15% of red foxes and in 63% of golden jackals. The findings emphasize the need for an active surveillance program for Trichinella spp. infection in wildlife in Serbia and the whole of the Balkans, with special attention on the red fox because it is widespread and occurs in high densities.",
publisher = "Amer Assoc Zoo Veterinarians, Yulee",
journal = "Journal of Zoo and Wildlife Medicine",
title = "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia",
volume = "48",
number = "3",
pages = "703-707",
doi = "10.1638/2016-0169.1"
}

Dmitrić, M., Vidanović, D., Vasković, N., Matović, K., Šekler, M., Debeljak, Z.,& Karabasil, N.. (2017). Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine
Amer Assoc Zoo Veterinarians, Yulee., 48(3), 703-707.
https://doi.org/10.1638/2016-0169.1

Dmitrić M, Vidanović D, Vasković N, Matović K, Šekler M, Debeljak Z, Karabasil N. Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia. in Journal of Zoo and Wildlife Medicine. 2017;48(3):703-707.
doi:10.1638/2016-0169.1 .

Dmitrić, Marko, Vidanović, Dejan, Vasković, Nikola, Matović, Kazimir, Šekler, Milanko, Debeljak, Zoran, Karabasil, Nedjeljko, "Trichinella infections in red foxes (vulpes vulpes) and golden jackals (canis aureus) in six districts of Serbia" in Journal of Zoo and Wildlife Medicine, 48, no. 3 (2017):703-707,
https://doi.org/10.1638/2016-0169.1 . .

TY  - JOUR
AU  - Bošnjak, Ivan
AU  - Zdravković, Nemanja
AU  - Čolović, Svetlana
AU  - Ranđelović, Slađana
AU  - Galić, Nataša
AU  - Radojičić, Marina
AU  - Šekler, Milanko
AU  - Aleksić-Kovačević, Sanja
AU  - Krnjaić, Dejan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1330
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia
T1  - Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji
VL  - 73
IS  - 10
SP  - 980
EP  - 982
DO  - 10.2298/VSP160809222B
ER  - 

@article{
author = "Bošnjak, Ivan and Zdravković, Nemanja and Čolović, Svetlana and Ranđelović, Slađana and Galić, Nataša and Radojičić, Marina and Šekler, Milanko and Aleksić-Kovačević, Sanja and Krnjaić, Dejan",
year = "2016",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia, Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji",
volume = "73",
number = "10",
pages = "980-982",
doi = "10.2298/VSP160809222B"
}

Bošnjak, I., Zdravković, N., Čolović, S., Ranđelović, S., Galić, N., Radojičić, M., Šekler, M., Aleksić-Kovačević, S.,& Krnjaić, D.. (2016). Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(10), 980-982.
https://doi.org/10.2298/VSP160809222B

Bošnjak I, Zdravković N, Čolović S, Ranđelović S, Galić N, Radojičić M, Šekler M, Aleksić-Kovačević S, Krnjaić D. Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled. 2016;73(10):980-982.
doi:10.2298/VSP160809222B .

Bošnjak, Ivan, Zdravković, Nemanja, Čolović, Svetlana, Ranđelović, Slađana, Galić, Nataša, Radojičić, Marina, Šekler, Milanko, Aleksić-Kovačević, Sanja, Krnjaić, Dejan, "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia" in Vojnosanitetski pregled, 73, no. 10 (2016):980-982,
https://doi.org/10.2298/VSP160809222B . .

TY  - JOUR
AU  - Vučićević, Miloš
AU  - Stevanović, Jevrosima
AU  - Šekler, Milanko
AU  - Resanović, Radmila
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1345
AB  - Determining the sex in birds is very difficult, primarily because over 50% of species is monomorphic (no morphological differences between the sexes). Before the application of molecular genetic methods, there were used numerous methods all of which were unreliable. Because of the importance of the analyses, they have to be reliable, economical, safe and prompt. Highly conserved CHD gene is defined in 1995. on W chromosome in birds, while on Z chromosome it was defined two years later. The difference in the length of the intronic sequences of CHD gene of Z and W chromosomes enables the distinguishing of the sexes after amplification of specific fragments by the application of specific primers. Molecular genetic methods have the supremacy over all the other methods because, except for being safe (both for birds and people), they provide reliable results, and also can be applied in all bird species.
AB  - Determinacija pola ptica je komplikovana, prevashodno jer je preko 50% vrsta monomorfno (nema morfoloških razlika između polova). Pre primene molekularno genetičkih metoda, koristile su se brojne metode čija je glavna karakteristika da su nepouzdane. Zbog značaja analiza, one moraju biti pouzdane, ekonomične, bezbedne i brze. Visokokonzervativni CHD gen je definisan 1995. godine na W hromozomu ptica, dok je na Z hromozomu definisan dve godine kasnije. Razlika u dužini intronskih sekvenci CHD gena Z i W hromozoma, omogućava razlikovanje polova nakon amplifikacije specifičnih fragmenata primenom specifičnih prajmera. Molekularno genetičke metode imaju primat nad svim ostalim metodama jer osim toga što su bezbedne za izvođenje (i po pticu i po čoveka), daju pouzdane rezultate, a mogu se koristiti kod svih vrsta ptica.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Historical overview of methods for sex determination in birds
T1  - Istorijski pregled metoda determinacije pola ptica
VL  - 70
IS  - 3-4
SP  - 145
EP  - 157
DO  - 10.2298/VETGL1604145V
ER  - 

@article{
author = "Vučićević, Miloš and Stevanović, Jevrosima and Šekler, Milanko and Resanović, Radmila and Stanimirović, Zoran",
year = "2016",
abstract = "Determining the sex in birds is very difficult, primarily because over 50% of species is monomorphic (no morphological differences between the sexes). Before the application of molecular genetic methods, there were used numerous methods all of which were unreliable. Because of the importance of the analyses, they have to be reliable, economical, safe and prompt. Highly conserved CHD gene is defined in 1995. on W chromosome in birds, while on Z chromosome it was defined two years later. The difference in the length of the intronic sequences of CHD gene of Z and W chromosomes enables the distinguishing of the sexes after amplification of specific fragments by the application of specific primers. Molecular genetic methods have the supremacy over all the other methods because, except for being safe (both for birds and people), they provide reliable results, and also can be applied in all bird species., Determinacija pola ptica je komplikovana, prevashodno jer je preko 50% vrsta monomorfno (nema morfoloških razlika između polova). Pre primene molekularno genetičkih metoda, koristile su se brojne metode čija je glavna karakteristika da su nepouzdane. Zbog značaja analiza, one moraju biti pouzdane, ekonomične, bezbedne i brze. Visokokonzervativni CHD gen je definisan 1995. godine na W hromozomu ptica, dok je na Z hromozomu definisan dve godine kasnije. Razlika u dužini intronskih sekvenci CHD gena Z i W hromozoma, omogućava razlikovanje polova nakon amplifikacije specifičnih fragmenata primenom specifičnih prajmera. Molekularno genetičke metode imaju primat nad svim ostalim metodama jer osim toga što su bezbedne za izvođenje (i po pticu i po čoveka), daju pouzdane rezultate, a mogu se koristiti kod svih vrsta ptica.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Historical overview of methods for sex determination in birds, Istorijski pregled metoda determinacije pola ptica",
volume = "70",
number = "3-4",
pages = "145-157",
doi = "10.2298/VETGL1604145V"
}

Vučićević, M., Stevanović, J., Šekler, M., Resanović, R.,& Stanimirović, Z.. (2016). Historical overview of methods for sex determination in birds. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3-4), 145-157.
https://doi.org/10.2298/VETGL1604145V

Vučićević M, Stevanović J, Šekler M, Resanović R, Stanimirović Z. Historical overview of methods for sex determination in birds. in Veterinarski Glasnik. 2016;70(3-4):145-157.
doi:10.2298/VETGL1604145V .

Vučićević, Miloš, Stevanović, Jevrosima, Šekler, Milanko, Resanović, Radmila, Stanimirović, Zoran, "Historical overview of methods for sex determination in birds" in Veterinarski Glasnik, 70, no. 3-4 (2016):145-157,
https://doi.org/10.2298/VETGL1604145V . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Petrić, Dušan
AU  - Vasić, Ana
AU  - Hrnjaković-Cvjetković, Ivana
AU  - Milošević, Vesna
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Lazić, Sava
AU  - Đuričić, Bosiljka
AU  - Plavšić, Budimir
AU  - Juan-Carlos, Saiz
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1205
AB  - West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed.
AB  - Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - WNV infection: An emergent vector borne viral infection in Serbia: Current situation
T1  - Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija
VL  - 69
IS  - 1-2
SP  - 111
EP  - 126
DO  - 10.2298/VETGL1502111P
ER  - 

@article{
author = "Petrović, Tamaš and Lupulović, Diana and Petrić, Dušan and Vasić, Ana and Hrnjaković-Cvjetković, Ivana and Milošević, Vesna and Vidanović, Dejan and Šekler, Milanko and Lazić, Sava and Đuričić, Bosiljka and Plavšić, Budimir and Juan-Carlos, Saiz",
year = "2015",
abstract = "West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed., Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "WNV infection: An emergent vector borne viral infection in Serbia: Current situation, Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija",
volume = "69",
number = "1-2",
pages = "111-126",
doi = "10.2298/VETGL1502111P"
}

Petrović, T., Lupulović, D., Petrić, D., Vasić, A., Hrnjaković-Cvjetković, I., Milošević, V., Vidanović, D., Šekler, M., Lazić, S., Đuričić, B., Plavšić, B.,& Juan-Carlos, S.. (2015). WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 111-126.
https://doi.org/10.2298/VETGL1502111P

Petrović T, Lupulović D, Petrić D, Vasić A, Hrnjaković-Cvjetković I, Milošević V, Vidanović D, Šekler M, Lazić S, Đuričić B, Plavšić B, Juan-Carlos S. WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik. 2015;69(1-2):111-126.
doi:10.2298/VETGL1502111P .

Petrović, Tamaš, Lupulović, Diana, Petrić, Dušan, Vasić, Ana, Hrnjaković-Cvjetković, Ivana, Milošević, Vesna, Vidanović, Dejan, Šekler, Milanko, Lazić, Sava, Đuričić, Bosiljka, Plavšić, Budimir, Juan-Carlos, Saiz, "WNV infection: An emergent vector borne viral infection in Serbia: Current situation" in Veterinarski Glasnik, 69, no. 1-2 (2015):111-126,
https://doi.org/10.2298/VETGL1502111P . .

TY  - CONF
AU  - Obradović, Saša
AU  - Šarčević, Branislav
AU  - Šekler, Milanko
AU  - Đekić, Vera
AU  - Dekić, Radoslav
AU  - Veljović, Nenad
AU  - Marković, Maja
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3862
AB  - Važan segment svakog upravljačkog sistema su ljudski resursi i pronalaženje modaliteta
da se isti koriste racionalno i ekonomično. Cilj rada je da se na konkretnom
primeru određivanja parametara koji utiču na optimalan broj ribočuvara po prvi put
prikaže mogućnost primene skalarnog metoda ocenjivanja (SMO) u praktičnom upravljanju
ribolovnim vodama. Kombinovanjem metoda SMO sa metodom analitičko hijerarhijskog
procesa, moguće je upravljačke odluke na objektivan način valorizovati i
učiniti metodološki primenljivim prilikom određivanja optimalnog broja ribočuvarske
službe. Objektivnost definisanja kriterijuma i izbor alternativa u odnosu na postavljeni
cilj zavise od dostupnih inicijalnih informacija i iskustva donosioca odluka, ali je ovaj
nedostatak moguće otkloniti donošenjem alternativnih rešenja zasnovanim na principima
višekriterijumske analize i matematičkog modelovanja.
U ovom radu SMO metod je prezentovan na primeru organizovanja ribočuvarske
službe i daje mogućnost da se ciljni parametri odrede u kvalitativnom i kvantitativnom
pogledu, kroz optimalizaciju broja potrebnih ribočuvara. Rezultati ovog rada ukazuju na
potrebu inoviranja postojećih metoda pri donošenju upravljačkih odluka po pitanju organizovanja ribočuvarske službe. Iako na prvi pogled ovaj metod izgleda komplikovan,
primenom odgovarajućeg softvera i korišćenjem tabelarnih kalkulatora, ovaj metod postaje
izuzetno primenljiv i efikasan u donošenju pravilnih i realnih zaključaka.
PB  - Belgrade : Faculty of Agriculture
C3  - VI International Conference „Water & Fish“, Belgrade, June, 12 - 14. 2013
T1  - Optimization model of fish guard service in order to protect aquatic systems
SP  - 164
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3862
ER  - 

@conference{
author = "Obradović, Saša and Šarčević, Branislav and Šekler, Milanko and Đekić, Vera and Dekić, Radoslav and Veljović, Nenad and Marković, Maja",
year = "2013",
abstract = "Važan segment svakog upravljačkog sistema su ljudski resursi i pronalaženje modaliteta
da se isti koriste racionalno i ekonomično. Cilj rada je da se na konkretnom
primeru određivanja parametara koji utiču na optimalan broj ribočuvara po prvi put
prikaže mogućnost primene skalarnog metoda ocenjivanja (SMO) u praktičnom upravljanju
ribolovnim vodama. Kombinovanjem metoda SMO sa metodom analitičko hijerarhijskog
procesa, moguće je upravljačke odluke na objektivan način valorizovati i
učiniti metodološki primenljivim prilikom određivanja optimalnog broja ribočuvarske
službe. Objektivnost definisanja kriterijuma i izbor alternativa u odnosu na postavljeni
cilj zavise od dostupnih inicijalnih informacija i iskustva donosioca odluka, ali je ovaj
nedostatak moguće otkloniti donošenjem alternativnih rešenja zasnovanim na principima
višekriterijumske analize i matematičkog modelovanja.
U ovom radu SMO metod je prezentovan na primeru organizovanja ribočuvarske
službe i daje mogućnost da se ciljni parametri odrede u kvalitativnom i kvantitativnom
pogledu, kroz optimalizaciju broja potrebnih ribočuvara. Rezultati ovog rada ukazuju na
potrebu inoviranja postojećih metoda pri donošenju upravljačkih odluka po pitanju organizovanja ribočuvarske službe. Iako na prvi pogled ovaj metod izgleda komplikovan,
primenom odgovarajućeg softvera i korišćenjem tabelarnih kalkulatora, ovaj metod postaje
izuzetno primenljiv i efikasan u donošenju pravilnih i realnih zaključaka.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "VI International Conference „Water & Fish“, Belgrade, June, 12 - 14. 2013",
title = "Optimization model of fish guard service in order to protect aquatic systems",
pages = "164-169",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3862"
}

Obradović, S., Šarčević, B., Šekler, M., Đekić, V., Dekić, R., Veljović, N.,& Marković, M.. (2013). Optimization model of fish guard service in order to protect aquatic systems. in VI International Conference „Water & Fish“, Belgrade, June, 12 - 14. 2013
Belgrade : Faculty of Agriculture., 164-169.
https://hdl.handle.net/21.15107/rcub_veterinar_3862

Obradović S, Šarčević B, Šekler M, Đekić V, Dekić R, Veljović N, Marković M. Optimization model of fish guard service in order to protect aquatic systems. in VI International Conference „Water & Fish“, Belgrade, June, 12 - 14. 2013. 2013;:164-169.
https://hdl.handle.net/21.15107/rcub_veterinar_3862 .

Obradović, Saša, Šarčević, Branislav, Šekler, Milanko, Đekić, Vera, Dekić, Radoslav, Veljović, Nenad, Marković, Maja, "Optimization model of fish guard service in order to protect aquatic systems" in VI International Conference „Water & Fish“, Belgrade, June, 12 - 14. 2013 (2013):164-169,
https://hdl.handle.net/21.15107/rcub_veterinar_3862 .

TY  - JOUR
AU  - Obradović, S.
AU  - Živković, B.
AU  - Đekić, V.
AU  - Šekler, Milanko
AU  - Živkov-Baloš, Milica
AU  - Marković, Maja
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1045
AB  - The effect of mannanoligo saccharides (MOS) as a food additive, applied at a concentration of 0.2% (O-I group of fish) and 0.3% (O-II group of fish) on morphometric characteristics and primary production of the Californian trout was investigated. The experiment was conducted on 450 fish divided into three groups with 150 individuals in each group, and lasted 40 days. The analysis of obtained results established the beneficial effect of the applied additives on morphometric parameters of the growth rate of fish, their final mass and final linear dimensions, and their growth, but no statistically significant differences (p> 0,05) were established. The highest body weight and body length of studied groups was achieved by trout of O-II group (118.49 g, 19.59 cm), followed by trout of O-I group (118.04 g, 19.58 cm) and the lowest K-group fish (115.94 g, 19.56 cm). Adding MOS in the feed mixture for trout had a beneficial effect (p> 0.05) in the exercise of lower feed conversion (FC) and better values of main production indicators: condition factor (FC), protein efficiency ratio (PER), the specific rate growth rate (SGR) and production index (PI) compared to the K group of fish that were fed diets without added mannanoligo saccharides. The best results were achieved by O- II fish group, and expressed in relative terms in comparison to the K group of fish: better feed conversion by 5.61%, a better utilization of protein feed value by 5.94%, a higher value of condition factor by 1.73 %, the specific growth rate by 2.26% and a better value of production index by 8.27%.
AB  - Ispitivan je uticaj mananoligosaharida (MOS) kao aditiva hrane, primenjenog u koncentraciji od 0,2% (O-I grupa riba) i 0,3% (O-II grupa riba) na morfometrijske karakteristike i osnovne proizvodne pokazatelje gajenja kalifornijske pastrmke. Ogled je sproveden na 450 riba podeljenih u tri grupe sa po 150 jedinki u svakoj grupi i trajao je 40 dana. Analizom dobijenih rezultata, ustanovljen je povoljan efekat primenjenog aditiva na morfometrijske pokazatelje tempa rasta riba, kako njihove završne komandne mase i završnih dužinskih mera, tako i njihovog prirasta, ali bez utvrđenih statistički značajnih razlika (p>0,05). Najveću prosečnu telesnu masu i dužinu tela kod ispitavanih grupa, ostvarile su pastrmke O-II grupe (118,49 g i 19,59 cm), zatim pastrmke O-I grupe (118,04 g i 19,58 cm), a najmanju K-grupa riba (115,94 g i 19,56 cm). Dodavanje MOS-a u smeše za ishranu pastrmki je imalo povoljan uticaj (p>0,05) na ostvarivanje niže konverzije hrane (HK) i boljih vrednosti osnovnih proizvodnih pokazatelja: faktora kondicije (FK), koeficijenta proteinske efikasnosti (PER), specifične stope rasta (SGR) i proizvodnog indeksa (PI) u odnosu na ribe K grupe, koje su hranjene smešama bez dodatka mananoligosaharida. Najbolje rezultate su ostvarile ribe O-II grupe, a iskazano u relativnim pokazateljima u odnosu na K grupu riba: bolju konverziju hrane za 5,61%; bolju vrednost iskorišćenja proteina hrane za 5,94%; veću vrednost faktora kondicije za 1,73%; specifične stopa rasta za 2,26% i bolju vrednost proizvodnog indeksa za 8,27%.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impact of mannanoligo saccharides on performance traits of rainbow trout
T1  - Uticaj mananoligosaharida na proizvodne osobine kalifornijske pastrmke
VL  - 29
IS  - 2
SP  - 353
EP  - 362
DO  - 10.2298/BAH1302353O
ER  - 

@article{
author = "Obradović, S. and Živković, B. and Đekić, V. and Šekler, Milanko and Živkov-Baloš, Milica and Marković, Maja",
year = "2013",
abstract = "The effect of mannanoligo saccharides (MOS) as a food additive, applied at a concentration of 0.2% (O-I group of fish) and 0.3% (O-II group of fish) on morphometric characteristics and primary production of the Californian trout was investigated. The experiment was conducted on 450 fish divided into three groups with 150 individuals in each group, and lasted 40 days. The analysis of obtained results established the beneficial effect of the applied additives on morphometric parameters of the growth rate of fish, their final mass and final linear dimensions, and their growth, but no statistically significant differences (p> 0,05) were established. The highest body weight and body length of studied groups was achieved by trout of O-II group (118.49 g, 19.59 cm), followed by trout of O-I group (118.04 g, 19.58 cm) and the lowest K-group fish (115.94 g, 19.56 cm). Adding MOS in the feed mixture for trout had a beneficial effect (p> 0.05) in the exercise of lower feed conversion (FC) and better values of main production indicators: condition factor (FC), protein efficiency ratio (PER), the specific rate growth rate (SGR) and production index (PI) compared to the K group of fish that were fed diets without added mannanoligo saccharides. The best results were achieved by O- II fish group, and expressed in relative terms in comparison to the K group of fish: better feed conversion by 5.61%, a better utilization of protein feed value by 5.94%, a higher value of condition factor by 1.73 %, the specific growth rate by 2.26% and a better value of production index by 8.27%., Ispitivan je uticaj mananoligosaharida (MOS) kao aditiva hrane, primenjenog u koncentraciji od 0,2% (O-I grupa riba) i 0,3% (O-II grupa riba) na morfometrijske karakteristike i osnovne proizvodne pokazatelje gajenja kalifornijske pastrmke. Ogled je sproveden na 450 riba podeljenih u tri grupe sa po 150 jedinki u svakoj grupi i trajao je 40 dana. Analizom dobijenih rezultata, ustanovljen je povoljan efekat primenjenog aditiva na morfometrijske pokazatelje tempa rasta riba, kako njihove završne komandne mase i završnih dužinskih mera, tako i njihovog prirasta, ali bez utvrđenih statistički značajnih razlika (p>0,05). Najveću prosečnu telesnu masu i dužinu tela kod ispitavanih grupa, ostvarile su pastrmke O-II grupe (118,49 g i 19,59 cm), zatim pastrmke O-I grupe (118,04 g i 19,58 cm), a najmanju K-grupa riba (115,94 g i 19,56 cm). Dodavanje MOS-a u smeše za ishranu pastrmki je imalo povoljan uticaj (p>0,05) na ostvarivanje niže konverzije hrane (HK) i boljih vrednosti osnovnih proizvodnih pokazatelja: faktora kondicije (FK), koeficijenta proteinske efikasnosti (PER), specifične stope rasta (SGR) i proizvodnog indeksa (PI) u odnosu na ribe K grupe, koje su hranjene smešama bez dodatka mananoligosaharida. Najbolje rezultate su ostvarile ribe O-II grupe, a iskazano u relativnim pokazateljima u odnosu na K grupu riba: bolju konverziju hrane za 5,61%; bolju vrednost iskorišćenja proteina hrane za 5,94%; veću vrednost faktora kondicije za 1,73%; specifične stopa rasta za 2,26% i bolju vrednost proizvodnog indeksa za 8,27%.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impact of mannanoligo saccharides on performance traits of rainbow trout, Uticaj mananoligosaharida na proizvodne osobine kalifornijske pastrmke",
volume = "29",
number = "2",
pages = "353-362",
doi = "10.2298/BAH1302353O"
}

Obradović, S., Živković, B., Đekić, V., Šekler, M., Živkov-Baloš, M.,& Marković, M.. (2013). Impact of mannanoligo saccharides on performance traits of rainbow trout. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(2), 353-362.
https://doi.org/10.2298/BAH1302353O

Obradović S, Živković B, Đekić V, Šekler M, Živkov-Baloš M, Marković M. Impact of mannanoligo saccharides on performance traits of rainbow trout. in Biotechnology in Animal Husbandry. 2013;29(2):353-362.
doi:10.2298/BAH1302353O .

Obradović, S., Živković, B., Đekić, V., Šekler, Milanko, Živkov-Baloš, Milica, Marković, Maja, "Impact of mannanoligo saccharides on performance traits of rainbow trout" in Biotechnology in Animal Husbandry, 29, no. 2 (2013):353-362,
https://doi.org/10.2298/BAH1302353O . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Lazić, S.
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Nišavić, Jakov
AU  - Resanović, Radmila
AU  - Petrović, Tamaš
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/864
AB  - Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NDV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains.
AB  - Pet sojeva virusa Newcastle bolesti (NDV) je izolovano iz uzoraka suspektnog materijala poreklom od živine 2006. i 2007.godine tokom epizootije atipične kuge živine na teritoriji Vojvodine, Srbija. Ovi izolati su potvrđeni i genotipski tipizirani primenom metoda RT-PCR uz korišćenje prajmera specifičnih za deo genoma virusa koji kodira sintezu fuzionog F proteina (572bp) i sekvenciranjem dela F gena sa filogenetskom analizom. Filogenetska analiza je ukazala da je svih pet izolovanih sojeva virusa Newcastle bolesti pripadalo genotipu VII. Istovremeno, svih pet sojeva je grupisano u podtip VIId navedenog virusa. Izolovani sojevi virusa Newcastle bolesti su međusobno bili veoma slični (99,7-100%) i grupisali su se sa sojevima virusa prethodno izolovanih iz divljih ptica u Srbiji tokom izbijanja bolesti 2006. i 2007. godine. Molekularnom karakterizacijom gena na mestu deobe fuzionog F proteina ustanovljeno je da svih pet izolovanih sojeva virusa pripada visoko virulentnim velogenim sojevima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia
T1  - Molekularna karakterizacija nekih sojeva virusa Newcastle bolesti izolovanih u Pokrajini Vojvodini Republike Srbije
VL  - 62
IS  - 4
SP  - 365
EP  - 374
DO  - 10.2298/AVB1204365M
ER  - 

@article{
author = "Milić, Nenad and Lazić, S. and Vidanović, Dejan and Šekler, Milanko and Nišavić, Jakov and Resanović, Radmila and Petrović, Tamaš",
year = "2012",
abstract = "Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NDV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains., Pet sojeva virusa Newcastle bolesti (NDV) je izolovano iz uzoraka suspektnog materijala poreklom od živine 2006. i 2007.godine tokom epizootije atipične kuge živine na teritoriji Vojvodine, Srbija. Ovi izolati su potvrđeni i genotipski tipizirani primenom metoda RT-PCR uz korišćenje prajmera specifičnih za deo genoma virusa koji kodira sintezu fuzionog F proteina (572bp) i sekvenciranjem dela F gena sa filogenetskom analizom. Filogenetska analiza je ukazala da je svih pet izolovanih sojeva virusa Newcastle bolesti pripadalo genotipu VII. Istovremeno, svih pet sojeva je grupisano u podtip VIId navedenog virusa. Izolovani sojevi virusa Newcastle bolesti su međusobno bili veoma slični (99,7-100%) i grupisali su se sa sojevima virusa prethodno izolovanih iz divljih ptica u Srbiji tokom izbijanja bolesti 2006. i 2007. godine. Molekularnom karakterizacijom gena na mestu deobe fuzionog F proteina ustanovljeno je da svih pet izolovanih sojeva virusa pripada visoko virulentnim velogenim sojevima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia, Molekularna karakterizacija nekih sojeva virusa Newcastle bolesti izolovanih u Pokrajini Vojvodini Republike Srbije",
volume = "62",
number = "4",
pages = "365-374",
doi = "10.2298/AVB1204365M"
}

Milić, N., Lazić, S., Vidanović, D., Šekler, M., Nišavić, J., Resanović, R.,& Petrović, T.. (2012). Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 365-374.
https://doi.org/10.2298/AVB1204365M

Milić N, Lazić S, Vidanović D, Šekler M, Nišavić J, Resanović R, Petrović T. Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia. in Acta Veterinaria-Beograd. 2012;62(4):365-374.
doi:10.2298/AVB1204365M .

Milić, Nenad, Lazić, S., Vidanović, Dejan, Šekler, Milanko, Nišavić, Jakov, Resanović, Radmila, Petrović, Tamaš, "Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia" in Acta Veterinaria-Beograd, 62, no. 4 (2012):365-374,
https://doi.org/10.2298/AVB1204365M . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/945
AB  - Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Application of standard and molecular methods for the diagnosis of Newcastle disease
VL  - 64
IS  - 4
SP  - 1433
EP  - 1437
DO  - 10.2298/ABS1204433V
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Polaček, Vladimir and Vasković, Nikola and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov",
year = "2012",
abstract = "Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Application of standard and molecular methods for the diagnosis of Newcastle disease",
volume = "64",
number = "4",
pages = "1433-1437",
doi = "10.2298/ABS1204433V"
}

Vidanović, D., Šekler, M., Polaček, V., Vasković, N., Ašanin, R., Milić, N.,& Nišavić, J.. (2012). Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1433-1437.
https://doi.org/10.2298/ABS1204433V

Vidanović D, Šekler M, Polaček V, Vasković N, Ašanin R, Milić N, Nišavić J. Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences. 2012;64(4):1433-1437.
doi:10.2298/ABS1204433V .

Vidanović, Dejan, Šekler, Milanko, Polaček, Vladimir, Vasković, Nikola, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, "Application of standard and molecular methods for the diagnosis of Newcastle disease" in Archives of Biological Sciences, 64, no. 4 (2012):1433-1437,
https://doi.org/10.2298/ABS1204433V . .

TY  - JOUR
AU  - Vasković, Nikola
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Polaček, Vladimir
AU  - Kukolj, Vladimir
AU  - Matović, Kazimir
AU  - Jovanović, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/803
AB  - The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined.
AB  - Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus
T1  - Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence
VL  - 61
IS  - 5-6
SP  - 591
EP  - 598
DO  - 10.2298/AVB1106591V
ER  - 

@article{
author = "Vasković, Nikola and Šekler, Milanko and Vidanović, Dejan and Polaček, Vladimir and Kukolj, Vladimir and Matović, Kazimir and Jovanović, M.",
year = "2011",
abstract = "The aim of this study was to determine pathomorphological lesions in the organs of birds infected with H5N1 avian influenza virus, presence and distribution of the viral antigen in tissue samples and the degree of their correlation. Examination was conducted on the carcasses of seven mute swans (Cygnus olor) and one domestic rooster (Gallus gallus domesticus). After necropsy, trachea, lungs, liver, spleen, pancreas, kidney and intestine tissues were sampled for histopathology and immunohistochemical examination. LSAB method and H5N1 avian influenza virus nucleoprotein polyclonal antibodies were used for detecting the viral antigen in the examined tissues. The most frequent gross lesions were hemorrhages and necroses of the pancreas. Major histological lesions were multifocal necroses in the pancreas, liver and spleen, non purulent encephalitis, with neuronal dystrophy and neuronophagia. Viral nucleoprotein was immunohistochemically proven in the pancreas, brain, liver, lungs, and in one swan in the kidney. A high correlation between pathomorphological lesions and distribution of the viral antigen was determined., Cilj ovog ispitivanja je bio utvrđivanje patomorfoloških promena na organima ptica inficiranih H5N1 virusom avijarne influence, imunohistohemijsko utvrđivanje prisustva i distribucije virusnog antigena u parafinskim isečcima uzoraka njihovih organa, kao i utvrđivanje stepena njihove korelacije. Ispitivanje je izvršeno na organima sedam labudova grbaca (Cygnus olor) i jednog petla (Gallus gallus domesticus). Nakon izvršene obdukcije i makroskopskog pregleda svih organskih sistema, izvršeno je uzorkovanje tkiva mozga, traheje, pluća, jetre, slezine, pankreasa, bubrega i creva za patohistološka i imunohistohemijska ispitivanja. Korišćena je LSAB imunohistohemijska metoda sa poliklonskim antitelima na nukleoprotein virusa avijarne influence podtipa H5N1. Dominantne promene utvrđene makrokopskim pregledom bile su krvavljenja i nekroze pankreasa. Histološkim pregledom su ustanovljene multifokalne nekroze pankreasa, jetre i slezine, a u velikom mozgu promene u tipu negnojnog encefalitisa, praćene distrofijom i nekrozom nervnih ćelija i pojavom neuronofagije. Virusni nukleoprotein je imunohistohemijski dokazan u pankreasu, mozgu, jetri, plućima, slezini, a kod jednog labuda i u bubrezima. Utvrđen je visok stepen korelacije između ustanovljenih patomorfoloških promena i distribucije virusnog antigena.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus, Patomorfološke promene i distribucija virusnog antigena kod ptica inficiranih patogenim sojem H5N1 virusa avijarne influence",
volume = "61",
number = "5-6",
pages = "591-598",
doi = "10.2298/AVB1106591V"
}

Vasković, N., Šekler, M., Vidanović, D., Polaček, V., Kukolj, V., Matović, K.,& Jovanović, M.. (2011). Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 591-598.
https://doi.org/10.2298/AVB1106591V

Vasković N, Šekler M, Vidanović D, Polaček V, Kukolj V, Matović K, Jovanović M. Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus. in Acta Veterinaria-Beograd. 2011;61(5-6):591-598.
doi:10.2298/AVB1106591V .

Vasković, Nikola, Šekler, Milanko, Vidanović, Dejan, Polaček, Vladimir, Kukolj, Vladimir, Matović, Kazimir, Jovanović, M., "Pathomorphological lesions and distribution of viral antigen in birds infected with the pathogenic strain of H5N1 avian influenza virus" in Acta Veterinaria-Beograd, 61, no. 5-6 (2011):591-598,
https://doi.org/10.2298/AVB1106591V . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Petrović, Tamaš
AU  - Savić, Vladimir
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/794
AB  - Avian paramyxoviruses type 1 or Newcastle disease viruses (NDV) are frequently recovered from wild birds and such isolates are most frequently of low virulence. Velogenic NDV are usually recovered from poultry and only occasionally from wild birds. Five NDV isolates were obtained from carcasses of four wild bird species during 2007 in Serbia: Mallard (Anas platyrhynchos), Eurasian Sparrowhawk (Accipiter nisus), feral Rock Pigeon (Columba livia), and Eurasian Collared Dove (Streptopelia decaocto). All the isolates have a typical fusion protein cleavage site motif of velogenic viruses (R-112-R-Q-K-R-F-117). The highest homology (99%) for the nucleotide sequences spanning the M and F gene of the studied isolates was with the genotype VII NDV isolate Muscovy duck/China(Fujian)/FP1/02. Phylogenetic analysis based on a partial F gene sequence showed that the isolates from wild birds cluster together with concurrent isolates from poultry in Serbia within the subgenotype VIId, which is the predominant pathogen involved currently in Newcastle disease outbreaks in poultry worldwide. It is unlikely that the wild birds played an important role in primary introduction or consequent spread of the velogenic NDV to domestic poultry in Serbia, and they probably contracted the virus from locally infected poultry.
PB  - Wildlife Disease Assoc, Inc, Lawrence
T2  - Journal of Wildlife Diseases
T1  - Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007
VL  - 47
IS  - 2
SP  - 433
EP  - 441
DO  - 10.7589/0090-3558-47.2.433
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov and Petrović, Tamaš and Savić, Vladimir",
year = "2011",
abstract = "Avian paramyxoviruses type 1 or Newcastle disease viruses (NDV) are frequently recovered from wild birds and such isolates are most frequently of low virulence. Velogenic NDV are usually recovered from poultry and only occasionally from wild birds. Five NDV isolates were obtained from carcasses of four wild bird species during 2007 in Serbia: Mallard (Anas platyrhynchos), Eurasian Sparrowhawk (Accipiter nisus), feral Rock Pigeon (Columba livia), and Eurasian Collared Dove (Streptopelia decaocto). All the isolates have a typical fusion protein cleavage site motif of velogenic viruses (R-112-R-Q-K-R-F-117). The highest homology (99%) for the nucleotide sequences spanning the M and F gene of the studied isolates was with the genotype VII NDV isolate Muscovy duck/China(Fujian)/FP1/02. Phylogenetic analysis based on a partial F gene sequence showed that the isolates from wild birds cluster together with concurrent isolates from poultry in Serbia within the subgenotype VIId, which is the predominant pathogen involved currently in Newcastle disease outbreaks in poultry worldwide. It is unlikely that the wild birds played an important role in primary introduction or consequent spread of the velogenic NDV to domestic poultry in Serbia, and they probably contracted the virus from locally infected poultry.",
publisher = "Wildlife Disease Assoc, Inc, Lawrence",
journal = "Journal of Wildlife Diseases",
title = "Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007",
volume = "47",
number = "2",
pages = "433-441",
doi = "10.7589/0090-3558-47.2.433"
}

Vidanović, D., Šekler, M., Ašanin, R., Milić, N., Nišavić, J., Petrović, T.,& Savić, V.. (2011). Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007. in Journal of Wildlife Diseases
Wildlife Disease Assoc, Inc, Lawrence., 47(2), 433-441.
https://doi.org/10.7589/0090-3558-47.2.433

Vidanović D, Šekler M, Ašanin R, Milić N, Nišavić J, Petrović T, Savić V. Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007. in Journal of Wildlife Diseases. 2011;47(2):433-441.
doi:10.7589/0090-3558-47.2.433 .

Vidanović, Dejan, Šekler, Milanko, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, Petrović, Tamaš, Savić, Vladimir, "Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007" in Journal of Wildlife Diseases, 47, no. 2 (2011):433-441,
https://doi.org/10.7589/0090-3558-47.2.433 . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Ašanin, Ružica
AU  - Knežević, Aleksandra
AU  - Ašanin, Jelena
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/746
AB  - Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
AB  - Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1
T1  - Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1
VL  - 64
IS  - 3-4
SP  - 159
EP  - 167
DO  - 10.2298/VETGL1004159M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Ašanin, Ružica and Knežević, Aleksandra and Ašanin, Jelena and Vidanović, Dejan and Šekler, Milanko",
year = "2010",
abstract = "Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method., Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1, Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1",
volume = "64",
number = "3-4",
pages = "159-167",
doi = "10.2298/VETGL1004159M"
}

Milić, N., Nišavić, J., Ašanin, R., Knežević, A., Ašanin, J., Vidanović, D.,& Šekler, M.. (2010). Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3-4), 159-167.
https://doi.org/10.2298/VETGL1004159M

Milić N, Nišavić J, Ašanin R, Knežević A, Ašanin J, Vidanović D, Šekler M. Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik. 2010;64(3-4):159-167.
doi:10.2298/VETGL1004159M .

Milić, Nenad, Nišavić, Jakov, Ašanin, Ružica, Knežević, Aleksandra, Ašanin, Jelena, Vidanović, Dejan, Šekler, Milanko, "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1" in Veterinarski Glasnik, 64, no. 3-4 (2010):159-167,
https://doi.org/10.2298/VETGL1004159M . .

TY  - CONF
AU  - Vasković, Nikola
AU  - Jovanović, M.
AU  - Polaček, Vladimir
AU  - Šekler, Milanko
AU  - Vidanović, Dejan
AU  - Tomić, A.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/735
PB  - Elsevier Sci Ltd, Oxford
C3  - Journal of Comparative Pathology
T1  - Lesions and distribution of viral antigen in birds infected with the pathogenic strain of h5n1 avian influenza virus
VL  - 143
IS  - 4
SP  - 330
EP  - 330
DO  - 10.1016/j.jcpa.2010.09.068
ER  - 

@conference{
author = "Vasković, Nikola and Jovanović, M. and Polaček, Vladimir and Šekler, Milanko and Vidanović, Dejan and Tomić, A.",
year = "2010",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Lesions and distribution of viral antigen in birds infected with the pathogenic strain of h5n1 avian influenza virus",
volume = "143",
number = "4",
pages = "330-330",
doi = "10.1016/j.jcpa.2010.09.068"
}

Vasković, N., Jovanović, M., Polaček, V., Šekler, M., Vidanović, D.,& Tomić, A.. (2010). Lesions and distribution of viral antigen in birds infected with the pathogenic strain of h5n1 avian influenza virus. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 143(4), 330-330.
https://doi.org/10.1016/j.jcpa.2010.09.068

Vasković N, Jovanović M, Polaček V, Šekler M, Vidanović D, Tomić A. Lesions and distribution of viral antigen in birds infected with the pathogenic strain of h5n1 avian influenza virus. in Journal of Comparative Pathology. 2010;143(4):330-330.
doi:10.1016/j.jcpa.2010.09.068 .

Vasković, Nikola, Jovanović, M., Polaček, Vladimir, Šekler, Milanko, Vidanović, Dejan, Tomić, A., "Lesions and distribution of viral antigen in birds infected with the pathogenic strain of h5n1 avian influenza virus" in Journal of Comparative Pathology, 143, no. 4 (2010):330-330,
https://doi.org/10.1016/j.jcpa.2010.09.068 . .

TY  - JOUR
AU  - Šekler, Milanko
AU  - Ašanin, Ružica
AU  - Krnjaić, Dejan
AU  - Palić, T.
AU  - Milić, Nenad
AU  - Jovanović, Tanja
AU  - Kovačević, Dragana
AU  - Plavšić, B.
AU  - Stojanović, Dragica
AU  - Vidanović, Dejan
AU  - Ašanin, N.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/652
AB  - Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%.
AB  - Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of presence of specific antibodies against avian influenza virus in some species of wild birds
T1  - Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica
VL  - 59
IS  - 4
SP  - 381
EP  - 403
DO  - 10.2298/AVB0904381S
ER  - 

@article{
author = "Šekler, Milanko and Ašanin, Ružica and Krnjaić, Dejan and Palić, T. and Milić, Nenad and Jovanović, Tanja and Kovačević, Dragana and Plavšić, B. and Stojanović, Dragica and Vidanović, Dejan and Ašanin, N.",
year = "2009",
abstract = "Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%., Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds, Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica",
volume = "59",
number = "4",
pages = "381-403",
doi = "10.2298/AVB0904381S"
}

Šekler, M., Ašanin, R., Krnjaić, D., Palić, T., Milić, N., Jovanović, T., Kovačević, D., Plavšić, B., Stojanović, D., Vidanović, D.,& Ašanin, N.. (2009). Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 381-403.
https://doi.org/10.2298/AVB0904381S

Šekler M, Ašanin R, Krnjaić D, Palić T, Milić N, Jovanović T, Kovačević D, Plavšić B, Stojanović D, Vidanović D, Ašanin N. Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd. 2009;59(4):381-403.
doi:10.2298/AVB0904381S .

Šekler, Milanko, Ašanin, Ružica, Krnjaić, Dejan, Palić, T., Milić, Nenad, Jovanović, Tanja, Kovačević, Dragana, Plavšić, B., Stojanović, Dragica, Vidanović, Dejan, Ašanin, N., "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds" in Acta Veterinaria-Beograd, 59, no. 4 (2009):381-403,
https://doi.org/10.2298/AVB0904381S . .