TY  - JOUR
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
AU  - Mišić, Dušan
AU  - Srejić, Damjan
AU  - Vasiljević, Dragan V.
AU  - Prokić, Kristina
AU  - Ilić, Nevenka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2630
AB  - It has long been known that coronaviruses cause various infectious diseases in animals. Although SARS-CoV-2 is genetically related to viruses isolated from Rhinolophus bats, the exact origin, mode of transmission, and how the human species has become the epidemiological reservoir of the virus have not yet been established with certainty. Although the main route of transmission is human-to-human, there are considerable numbers of reported cases of infection in animal species, predominantly among pet animals. The aim of this retrospective study was to assess SARS-CoV-2 seropositivity in dogs and cats during the COVID-19 pandemic in Šumadija District, Serbia. We used serology to identify household contacts of pet animals with infected pet owners and the degree of association. The study presented in this paper is also the first study of this type in Serbia. The results of a retrospective serosurvey, which was conducted in dogs and cats with different exposure risk factors, were analyzed to find the possible modes of transmission between humans and animals. The relative frequency of SARS-CoV-2 infection in dogs was 1.45% bounded with a 95% confidence interval (CI) of 0.0007–7.73%, while in cats, it was 5.56% (95% CI: 0.77–4.13%). The relative frequency of SARS-CoV-2 infection in pet owners was 11% (95% CI: 6.25–18.63%). In pets that were in close contact with COVID-19 positive owners, the seropositivity was found to be 9%. Out of a total of five stray dogs and cats tested, seropositivity was observed in two animals. Detected SARS-CoV-2 infection in pets shows that these animals are susceptible to infection and that the most common means of virus transmission to pets is through contact with diseased owners. However, the presence of infection in stray dogs and cats is not clear and needs further research.
PB  - Elsevier B.V.
T2  - Preventive Veterinary Medicine
T1  - Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia
VL  - 208
DO  - 10.1016/j.prevetmed.2022.105755
ER  - 

@article{
author = "Stanojević, Slavoljub and Radojičić, Sonja and Mišić, Dušan and Srejić, Damjan and Vasiljević, Dragan V. and Prokić, Kristina and Ilić, Nevenka",
year = "2022",
abstract = "It has long been known that coronaviruses cause various infectious diseases in animals. Although SARS-CoV-2 is genetically related to viruses isolated from Rhinolophus bats, the exact origin, mode of transmission, and how the human species has become the epidemiological reservoir of the virus have not yet been established with certainty. Although the main route of transmission is human-to-human, there are considerable numbers of reported cases of infection in animal species, predominantly among pet animals. The aim of this retrospective study was to assess SARS-CoV-2 seropositivity in dogs and cats during the COVID-19 pandemic in Šumadija District, Serbia. We used serology to identify household contacts of pet animals with infected pet owners and the degree of association. The study presented in this paper is also the first study of this type in Serbia. The results of a retrospective serosurvey, which was conducted in dogs and cats with different exposure risk factors, were analyzed to find the possible modes of transmission between humans and animals. The relative frequency of SARS-CoV-2 infection in dogs was 1.45% bounded with a 95% confidence interval (CI) of 0.0007–7.73%, while in cats, it was 5.56% (95% CI: 0.77–4.13%). The relative frequency of SARS-CoV-2 infection in pet owners was 11% (95% CI: 6.25–18.63%). In pets that were in close contact with COVID-19 positive owners, the seropositivity was found to be 9%. Out of a total of five stray dogs and cats tested, seropositivity was observed in two animals. Detected SARS-CoV-2 infection in pets shows that these animals are susceptible to infection and that the most common means of virus transmission to pets is through contact with diseased owners. However, the presence of infection in stray dogs and cats is not clear and needs further research.",
publisher = "Elsevier B.V.",
journal = "Preventive Veterinary Medicine",
title = "Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia",
volume = "208",
doi = "10.1016/j.prevetmed.2022.105755"
}

Stanojević, S., Radojičić, S., Mišić, D., Srejić, D., Vasiljević, D. V., Prokić, K.,& Ilić, N.. (2022). Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia. in Preventive Veterinary Medicine
Elsevier B.V.., 208.
https://doi.org/10.1016/j.prevetmed.2022.105755

Stanojević S, Radojičić S, Mišić D, Srejić D, Vasiljević DV, Prokić K, Ilić N. Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia. in Preventive Veterinary Medicine. 2022;208.
doi:10.1016/j.prevetmed.2022.105755 .

Stanojević, Slavoljub, Radojičić, Sonja, Mišić, Dušan, Srejić, Damjan, Vasiljević, Dragan V., Prokić, Kristina, Ilić, Nevenka, "Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia" in Preventive Veterinary Medicine, 208 (2022),
https://doi.org/10.1016/j.prevetmed.2022.105755 . .

TY  - JOUR
AU  - Ilić Božović, Anja
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Tyrrell, Phyllis
AU  - Chandrashekar, Ramaswamy
AU  - Mišić, Dušan
AU  - Kovačević-Filipović, Milica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2043
AB  - In September 2018, a four-month-old dog with fever and petechial bleeding came to the internal clinic at the Faculty of Veterinary Medicine University of Belgrade. On hematology analysis, thrombocytopenia and mild anemia were observed. Examination of the blood smear revealed platelet inclusions. The commercial serology test was positive for Anaplasma spp. The dog was treated with doxycycline for 14 days, and after 48 hours from the beginning of the treatment, the symptoms subsided. PCR analysis and sequencing confirmed infection with A. platys.
AB  - U septembru 2018. godine, štene staro četiri meseca, sa znacima groznice i petehijalnim krvarenjem, je došlo u ambulantu Klinike za male životinje Fakulteta veterinarske medicine Univerziteta u Beogradu. Hematološka analiza je pokazala trombocitopeniju i blagu anemiju. Pregledom krvnog razmaza ustanovljene su inkluzije u trombocitima. Komercijalni serološki test je pokazao prisustvo antitela protiv Anaplasma spp. Sprovedena je terapija doksiciklinom u trajanju od 14 dana, a posle 48 sati od početka terapije znaci bolesti su se povukli. PCR analizom i senkvencioniranjem je potvrđeno prisustvo Anaplasma platys.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - First confirmed clinical case of Anaplasma platys in a dog in Serbia
T1  - Prvi potvrđeni klinički slučaj infekcije psa vrstom Anaplasma platys u Srbiji
VL  - 71
IS  - 1
SP  - 107
EP  - 112
DO  - 10.2478/acve-2021-0008
ER  - 

@article{
author = "Ilić Božović, Anja and Radaković, Milena and Spariosu, Kristina and Tyrrell, Phyllis and Chandrashekar, Ramaswamy and Mišić, Dušan and Kovačević-Filipović, Milica",
year = "2021",
abstract = "In September 2018, a four-month-old dog with fever and petechial bleeding came to the internal clinic at the Faculty of Veterinary Medicine University of Belgrade. On hematology analysis, thrombocytopenia and mild anemia were observed. Examination of the blood smear revealed platelet inclusions. The commercial serology test was positive for Anaplasma spp. The dog was treated with doxycycline for 14 days, and after 48 hours from the beginning of the treatment, the symptoms subsided. PCR analysis and sequencing confirmed infection with A. platys., U septembru 2018. godine, štene staro četiri meseca, sa znacima groznice i petehijalnim krvarenjem, je došlo u ambulantu Klinike za male životinje Fakulteta veterinarske medicine Univerziteta u Beogradu. Hematološka analiza je pokazala trombocitopeniju i blagu anemiju. Pregledom krvnog razmaza ustanovljene su inkluzije u trombocitima. Komercijalni serološki test je pokazao prisustvo antitela protiv Anaplasma spp. Sprovedena je terapija doksiciklinom u trajanju od 14 dana, a posle 48 sati od početka terapije znaci bolesti su se povukli. PCR analizom i senkvencioniranjem je potvrđeno prisustvo Anaplasma platys.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "First confirmed clinical case of Anaplasma platys in a dog in Serbia, Prvi potvrđeni klinički slučaj infekcije psa vrstom Anaplasma platys u Srbiji",
volume = "71",
number = "1",
pages = "107-112",
doi = "10.2478/acve-2021-0008"
}

Ilić Božović, A., Radaković, M., Spariosu, K., Tyrrell, P., Chandrashekar, R., Mišić, D.,& Kovačević-Filipović, M.. (2021). First confirmed clinical case of Anaplasma platys in a dog in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 107-112.
https://doi.org/10.2478/acve-2021-0008

Ilić Božović A, Radaković M, Spariosu K, Tyrrell P, Chandrashekar R, Mišić D, Kovačević-Filipović M. First confirmed clinical case of Anaplasma platys in a dog in Serbia. in Acta Veterinaria-Beograd. 2021;71(1):107-112.
doi:10.2478/acve-2021-0008 .

Ilić Božović, Anja, Radaković, Milena, Spariosu, Kristina, Tyrrell, Phyllis, Chandrashekar, Ramaswamy, Mišić, Dušan, Kovačević-Filipović, Milica, "First confirmed clinical case of Anaplasma platys in a dog in Serbia" in Acta Veterinaria-Beograd, 71, no. 1 (2021):107-112,
https://doi.org/10.2478/acve-2021-0008 . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Tadić, Vanja
AU  - Korzeniowska, Malgorzata
AU  - Nišavić, Jakov
AU  - Aksentijević, Ksenija
AU  - Kuzmanovic, Jelena
AU  - Žižović, Irena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1855
AB  - Supercritical fluid extraction as an environmentally friendly technology was applied to isolate biologically active extracts from celery and parsley fruits for potential applications in the food industry. The extractions were performed under mild temperature conditions of 39.85 °C and at pressures of 10 and 30 MPa. The extracts were analyzed regarding their chemical composition, antibacterial activity, and cytotoxic effect. Sedanolide was the dominant component of the celery fruit extracts, comprising more than 70% of the obtained fraction, while the content of apiole in the parsley fruit SC CO2 extracts exceeded 85%. The celery fruit extracts showed strong and moderately strong antibacterial activity against tested Staphylococcus aureus, Bacillus (B.) cereus, B. subtilis, B. circulans, Listeria (L.) greyi, L. seeligeri and L. welshimeri, with minimal inhibitory concentration (MIC) values between 160 and 640 µg/mL, and weak activity against the selected Salmonella isolates with a MIC of 2560 µg/mL. The parsley extract obtained at 10 MPa showed strong and moderately strong antibacterial effects against Bacillus strains with obtained MICs of 160-640 µg/mL, and weak activity against Staphylococcus, Listeria, and Salmonella with a MIC of 2560 µg/mL. Cytotoxicity investigation showed that the extracts with proven antibacterial activity had no cytotoxic effect on rabbit kidney cells at concentrations of up to 640 µg/mL.
PB  - MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity
VL  - 25
IS  - 14
DO  - 10.3390/molecules25143163
ER  - 

@article{
author = "Mišić, Dušan and Tadić, Vanja and Korzeniowska, Malgorzata and Nišavić, Jakov and Aksentijević, Ksenija and Kuzmanovic, Jelena and Žižović, Irena",
year = "2020",
abstract = "Supercritical fluid extraction as an environmentally friendly technology was applied to isolate biologically active extracts from celery and parsley fruits for potential applications in the food industry. The extractions were performed under mild temperature conditions of 39.85 °C and at pressures of 10 and 30 MPa. The extracts were analyzed regarding their chemical composition, antibacterial activity, and cytotoxic effect. Sedanolide was the dominant component of the celery fruit extracts, comprising more than 70% of the obtained fraction, while the content of apiole in the parsley fruit SC CO2 extracts exceeded 85%. The celery fruit extracts showed strong and moderately strong antibacterial activity against tested Staphylococcus aureus, Bacillus (B.) cereus, B. subtilis, B. circulans, Listeria (L.) greyi, L. seeligeri and L. welshimeri, with minimal inhibitory concentration (MIC) values between 160 and 640 µg/mL, and weak activity against the selected Salmonella isolates with a MIC of 2560 µg/mL. The parsley extract obtained at 10 MPa showed strong and moderately strong antibacterial effects against Bacillus strains with obtained MICs of 160-640 µg/mL, and weak activity against Staphylococcus, Listeria, and Salmonella with a MIC of 2560 µg/mL. Cytotoxicity investigation showed that the extracts with proven antibacterial activity had no cytotoxic effect on rabbit kidney cells at concentrations of up to 640 µg/mL.",
publisher = "MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity",
volume = "25",
number = "14",
doi = "10.3390/molecules25143163"
}

Mišić, D., Tadić, V., Korzeniowska, M., Nišavić, J., Aksentijević, K., Kuzmanovic, J.,& Žižović, I.. (2020). Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity. in Molecules (Basel, Switzerland)
MDPI., 25(14).
https://doi.org/10.3390/molecules25143163

Mišić D, Tadić V, Korzeniowska M, Nišavić J, Aksentijević K, Kuzmanovic J, Žižović I. Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity. in Molecules (Basel, Switzerland). 2020;25(14).
doi:10.3390/molecules25143163 .

Mišić, Dušan, Tadić, Vanja, Korzeniowska, Malgorzata, Nišavić, Jakov, Aksentijević, Ksenija, Kuzmanovic, Jelena, Žižović, Irena, "Supercritical Fluid Extraction of Celery and Parsley Fruit-Chemical Composition and Antibacterial Activity" in Molecules (Basel, Switzerland), 25, no. 14 (2020),
https://doi.org/10.3390/molecules25143163 . .

TY  - JOUR
AU  - Marković, D.
AU  - Vasiljević, J.
AU  - Ašanin, Jelena
AU  - Ilic-Tomić, T.
AU  - Tomšič, B.
AU  - Jokić, B.
AU  - Mitrić, M.
AU  - Simončič, B.
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1824
AB  - A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol–gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol–gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO4 solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negative Escherichia coli ATCC 25922, E. coli ATCC BAA 2469, and Klebsiella pneumoniae ATCC BAA 2146, and Gram-positive bacteria Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 and yeast Candida albicans ATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.
PB  - John Wiley and Sons Inc.
T2  - Journal of Applied Polymer Science
T1  - The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions
DO  - 10.1002/app.49194
ER  - 

@article{
author = "Marković, D. and Vasiljević, J. and Ašanin, Jelena and Ilic-Tomić, T. and Tomšič, B. and Jokić, B. and Mitrić, M. and Simončič, B. and Mišić, Dušan and Radetić, Maja",
year = "2020",
abstract = "A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol–gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol–gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO4 solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negative Escherichia coli ATCC 25922, E. coli ATCC BAA 2469, and Klebsiella pneumoniae ATCC BAA 2146, and Gram-positive bacteria Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 and yeast Candida albicans ATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.",
publisher = "John Wiley and Sons Inc.",
journal = "Journal of Applied Polymer Science",
title = "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions",
doi = "10.1002/app.49194"
}

Marković, D., Vasiljević, J., Ašanin, J., Ilic-Tomić, T., Tomšič, B., Jokić, B., Mitrić, M., Simončič, B., Mišić, D.,& Radetić, M.. (2020). The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science
John Wiley and Sons Inc...
https://doi.org/10.1002/app.49194

Marković D, Vasiljević J, Ašanin J, Ilic-Tomić T, Tomšič B, Jokić B, Mitrić M, Simončič B, Mišić D, Radetić M. The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science. 2020;.
doi:10.1002/app.49194 .

Marković, D., Vasiljević, J., Ašanin, Jelena, Ilic-Tomić, T., Tomšič, B., Jokić, B., Mitrić, M., Simončič, B., Mišić, Dušan, Radetić, Maja, "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions" in Journal of Applied Polymer Science (2020),
https://doi.org/10.1002/app.49194 . .

TY  - JOUR
AU  - Loncaric, Igor
AU  - Mišić, Dušan
AU  - Szostak, Michael P.
AU  - Künzel, Frank
AU  - Schäfer-Somi, Sabine
AU  - Spergser, Joachim
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1853
AB  - The aim of the present study was to characterize Enterobacterales resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains (Escherichia coli (n = 28), Klebsiella pneumoniae (n = 3), Serratia marcescens, Raoultella ornithinolytica, Proteus mirabilis, Citrobacter portucalensis and Enterobacter cloacae (each n = 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno-and genotyping and microarray-based assays. Escherichia (E.) coli isolates were additionally characterized by two-locus (fumC and fimH) sequence phylotyping and multi-locus sequence typing (MLST) of selected isolates. MLST of carbapenem-resistant Enterobacter cloacae isolates was also performed. Among E. coli, the most dominant phylogenetic group was B1 (27.8%), followed by C, (16.6%), A and Clade II (5.5% each), B2 and F (2.77% each). The most predominant β-lactam resistance genes were blaTEM (70%) and blaCTX-M (38.8%), blaCMY (25%). blaNDM was detected in one carbapenem-resistant Enterobacter cloacae ST114. The most common ST among selected E. coli was 744 (10.7% isolates). The pandemic clones ST131 and ST648 carrying CTX-M-15 were also detected. Remaining STs belonged to 469, 1287, 1463 and 1642. E. coli clonotyping revealed 20 CH types. Based on the presence of certain virulence genes, three isolates were categorized as ExPEC/UPEC. The most prevalent virulence factors were fimH detected in 61%, iucD and iss both in 55%, iroN in 27.8%, papC in 13.8% and sat in 8.3% isolates.
PB  - MDPI
T2  - Antibiotics
T1  - Broad-spectrum cephalosporin-resistant and/or fluoroquinolone-resistant enterobacterales associated with canine and feline urogenital infections
VL  - 9
IS  - 7
SP  - 387
DO  - 10.3390/antibiotics9070387
ER  - 

@article{
author = "Loncaric, Igor and Mišić, Dušan and Szostak, Michael P. and Künzel, Frank and Schäfer-Somi, Sabine and Spergser, Joachim",
year = "2020",
abstract = "The aim of the present study was to characterize Enterobacterales resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains (Escherichia coli (n = 28), Klebsiella pneumoniae (n = 3), Serratia marcescens, Raoultella ornithinolytica, Proteus mirabilis, Citrobacter portucalensis and Enterobacter cloacae (each n = 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno-and genotyping and microarray-based assays. Escherichia (E.) coli isolates were additionally characterized by two-locus (fumC and fimH) sequence phylotyping and multi-locus sequence typing (MLST) of selected isolates. MLST of carbapenem-resistant Enterobacter cloacae isolates was also performed. Among E. coli, the most dominant phylogenetic group was B1 (27.8%), followed by C, (16.6%), A and Clade II (5.5% each), B2 and F (2.77% each). The most predominant β-lactam resistance genes were blaTEM (70%) and blaCTX-M (38.8%), blaCMY (25%). blaNDM was detected in one carbapenem-resistant Enterobacter cloacae ST114. The most common ST among selected E. coli was 744 (10.7% isolates). The pandemic clones ST131 and ST648 carrying CTX-M-15 were also detected. Remaining STs belonged to 469, 1287, 1463 and 1642. E. coli clonotyping revealed 20 CH types. Based on the presence of certain virulence genes, three isolates were categorized as ExPEC/UPEC. The most prevalent virulence factors were fimH detected in 61%, iucD and iss both in 55%, iroN in 27.8%, papC in 13.8% and sat in 8.3% isolates.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Broad-spectrum cephalosporin-resistant and/or fluoroquinolone-resistant enterobacterales associated with canine and feline urogenital infections",
volume = "9",
number = "7",
pages = "387",
doi = "10.3390/antibiotics9070387"
}

Loncaric, I., Mišić, D., Szostak, M. P., Künzel, F., Schäfer-Somi, S.,& Spergser, J.. (2020). Broad-spectrum cephalosporin-resistant and/or fluoroquinolone-resistant enterobacterales associated with canine and feline urogenital infections. in Antibiotics
MDPI., 9(7), 387.
https://doi.org/10.3390/antibiotics9070387

Loncaric I, Mišić D, Szostak MP, Künzel F, Schäfer-Somi S, Spergser J. Broad-spectrum cephalosporin-resistant and/or fluoroquinolone-resistant enterobacterales associated with canine and feline urogenital infections. in Antibiotics. 2020;9(7):387.
doi:10.3390/antibiotics9070387 .

Loncaric, Igor, Mišić, Dušan, Szostak, Michael P., Künzel, Frank, Schäfer-Somi, Sabine, Spergser, Joachim, "Broad-spectrum cephalosporin-resistant and/or fluoroquinolone-resistant enterobacterales associated with canine and feline urogenital infections" in Antibiotics, 9, no. 7 (2020):387,
https://doi.org/10.3390/antibiotics9070387 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Lukić, Ivana
AU  - Dikić, Jelena
AU  - Ašanin, Jelena
AU  - Gordić, Milan
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Korzeniowska, Malgorzata
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1881
AB  - In the present study, supercritical solvent impregnation (SSI) has been applied to incorporate thymol into bio-composite polymers as a potential active packaging material. Thymol, a natural component with a proven antimicrobial activity, was successfully impregnated into starch-chitosan (SC) and starch-chitosan-zeolite (SCZ) films using supercritical carbon dioxide (scCO2) as a solvent. Experiments were performed at 35 °C, pressures of 15.5 and 30 MPa, and an impregnation time in the range of 4–24 h. The highest impregnation yields of SC films with starch to chitosan mass ratios of 1:1 and 1:2 were 10.80% and 6.48%, respectively. The addition of natural zeolite (15–60%) significantly increased the loading capacity of films enabling thymol incorporation in a quantity of 16.7–27.3%. FTIR and SEM analyses were applied for the characterization of the films. Mechanical properties and water vapor permeability of films before and after the impregnation were tested as well. Thymol release kinetics in deionized water was followed and modeled by the Korsmeyer-Peppas and Weibull model. SCZ films with thymol loading of approximately 24% exhibited strong antibacterial activity against E. coli and methicillin-resistant Staphylococcus (S.) aureus (MRSA).
PB  - MDPI
T2  - Molecules
T1  - Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties
VL  - 25
IS  - 20
SP  - 4717
DO  - 10.3390/molecules25204717
ER  - 

@article{
author = "Pajnik, Jelena and Lukić, Ivana and Dikić, Jelena and Ašanin, Jelena and Gordić, Milan and Mišić, Dušan and Žižović, Irena and Korzeniowska, Malgorzata",
year = "2020",
abstract = "In the present study, supercritical solvent impregnation (SSI) has been applied to incorporate thymol into bio-composite polymers as a potential active packaging material. Thymol, a natural component with a proven antimicrobial activity, was successfully impregnated into starch-chitosan (SC) and starch-chitosan-zeolite (SCZ) films using supercritical carbon dioxide (scCO2) as a solvent. Experiments were performed at 35 °C, pressures of 15.5 and 30 MPa, and an impregnation time in the range of 4–24 h. The highest impregnation yields of SC films with starch to chitosan mass ratios of 1:1 and 1:2 were 10.80% and 6.48%, respectively. The addition of natural zeolite (15–60%) significantly increased the loading capacity of films enabling thymol incorporation in a quantity of 16.7–27.3%. FTIR and SEM analyses were applied for the characterization of the films. Mechanical properties and water vapor permeability of films before and after the impregnation were tested as well. Thymol release kinetics in deionized water was followed and modeled by the Korsmeyer-Peppas and Weibull model. SCZ films with thymol loading of approximately 24% exhibited strong antibacterial activity against E. coli and methicillin-resistant Staphylococcus (S.) aureus (MRSA).",
publisher = "MDPI",
journal = "Molecules",
title = "Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties",
volume = "25",
number = "20",
pages = "4717",
doi = "10.3390/molecules25204717"
}

Pajnik, J., Lukić, I., Dikić, J., Ašanin, J., Gordić, M., Mišić, D., Žižović, I.,& Korzeniowska, M.. (2020). Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties. in Molecules
MDPI., 25(20), 4717.
https://doi.org/10.3390/molecules25204717

Pajnik J, Lukić I, Dikić J, Ašanin J, Gordić M, Mišić D, Žižović I, Korzeniowska M. Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties. in Molecules. 2020;25(20):4717.
doi:10.3390/molecules25204717 .

Pajnik, Jelena, Lukić, Ivana, Dikić, Jelena, Ašanin, Jelena, Gordić, Milan, Mišić, Dušan, Žižović, Irena, Korzeniowska, Malgorzata, "Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties" in Molecules, 25, no. 20 (2020):4717,
https://doi.org/10.3390/molecules25204717 . .

TY  - JOUR
AU  - Krstić, Milena
AU  - Petković, Branka B.
AU  - Milcić, Milos
AU  - Mišić, Dušan
AU  - Francisco Santibanez, Juan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1752
AB  - Two new nitrato complexes of zinc and nickel with 1,4,8,11-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), have been synthesized and characterized. The IR spectral peaks showed that the coordinated and ionic nitrate ions are in agreement with the formula proposed by elemental analysis. Conductometric titrations predicted methanol to be a convenient solvent for synthesis and revealed the stoichiometry of the complexes, while molar electrical conductivities indicated a 1 : 3 complex electrolyte type for the zinc complex, and a 1 : 2 complex electrolyte type for the nickel complex. The optimized complex structure was obtained by molecular modeling and density functional theory calculations. The biological activity of the novel complexes was examined by screening eight different bacterial strains and two cancer cell lines. The zinc complex showed better antimicrobial activity against the bacterial strains, while the complexes did not show significance antiproliferative activity toward cancer cells MCF-7 and MDA-MB-231.
PB  - Soc Chemists Technologists Madeconia, Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Synthesis, characterization and biological study of new dinuclear zinc(ii) and nickel(ii) octaaza macrocyclic complexes
VL  - 38
IS  - 1
SP  - 1
EP  - 11
DO  - 10.20450/mjcce.2019.1599
ER  - 

@article{
author = "Krstić, Milena and Petković, Branka B. and Milcić, Milos and Mišić, Dušan and Francisco Santibanez, Juan",
year = "2019",
abstract = "Two new nitrato complexes of zinc and nickel with 1,4,8,11-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), have been synthesized and characterized. The IR spectral peaks showed that the coordinated and ionic nitrate ions are in agreement with the formula proposed by elemental analysis. Conductometric titrations predicted methanol to be a convenient solvent for synthesis and revealed the stoichiometry of the complexes, while molar electrical conductivities indicated a 1 : 3 complex electrolyte type for the zinc complex, and a 1 : 2 complex electrolyte type for the nickel complex. The optimized complex structure was obtained by molecular modeling and density functional theory calculations. The biological activity of the novel complexes was examined by screening eight different bacterial strains and two cancer cell lines. The zinc complex showed better antimicrobial activity against the bacterial strains, while the complexes did not show significance antiproliferative activity toward cancer cells MCF-7 and MDA-MB-231.",
publisher = "Soc Chemists Technologists Madeconia, Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Synthesis, characterization and biological study of new dinuclear zinc(ii) and nickel(ii) octaaza macrocyclic complexes",
volume = "38",
number = "1",
pages = "1-11",
doi = "10.20450/mjcce.2019.1599"
}

Krstić, M., Petković, B. B., Milcić, M., Mišić, D.,& Francisco Santibanez, J.. (2019). Synthesis, characterization and biological study of new dinuclear zinc(ii) and nickel(ii) octaaza macrocyclic complexes. in Macedonian Journal of Chemistry and Chemical Engineering
Soc Chemists Technologists Madeconia, Skopje., 38(1), 1-11.
https://doi.org/10.20450/mjcce.2019.1599

Krstić M, Petković BB, Milcić M, Mišić D, Francisco Santibanez J. Synthesis, characterization and biological study of new dinuclear zinc(ii) and nickel(ii) octaaza macrocyclic complexes. in Macedonian Journal of Chemistry and Chemical Engineering. 2019;38(1):1-11.
doi:10.20450/mjcce.2019.1599 .

Krstić, Milena, Petković, Branka B., Milcić, Milos, Mišić, Dušan, Francisco Santibanez, Juan, "Synthesis, characterization and biological study of new dinuclear zinc(ii) and nickel(ii) octaaza macrocyclic complexes" in Macedonian Journal of Chemistry and Chemical Engineering, 38, no. 1 (2019):1-11,
https://doi.org/10.20450/mjcce.2019.1599 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Mišić, Dušan
AU  - Karabasil, Nedjeljko
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Jevtić, Goran
AU  - Ćirić, Jelena
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1756
AB  - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Clostridium botulinum spores in European honey bees from Serbia
VL  - 58
IS  - 3
SP  - 420
EP  - 426
DO  - 10.1080/00218839.2018.1560654
ER  - 

@article{
author = "Matović, Kazimir and Mišić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena",
year = "2019",
abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Clostridium botulinum spores in European honey bees from Serbia",
volume = "58",
number = "3",
pages = "420-426",
doi = "10.1080/00218839.2018.1560654"
}

Matović, K., Mišić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 58(3), 420-426.
https://doi.org/10.1080/00218839.2018.1560654

Matović K, Mišić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426.
doi:10.1080/00218839.2018.1560654 .

Matović, Kazimir, Mišić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426,
https://doi.org/10.1080/00218839.2018.1560654 . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Aksentijević, Ksenija
AU  - Tambur, Zoran
AU  - Rakonjac, Bojan
AU  - Kovačević, Ivana
AU  - Spergser, Joachim
AU  - Loncarić, Igor
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1758
AB  - The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
VL  - 8
IS  - 1
SP  - 26
DO  - 10.3390/antibiotics8010026
ER  - 

@article{
author = "Ašanin, Jelena and Mišić, Dušan and Aksentijević, Ksenija and Tambur, Zoran and Rakonjac, Bojan and Kovačević, Ivana and Spergser, Joachim and Loncarić, Igor",
year = "2019",
abstract = "The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia",
volume = "8",
number = "1",
pages = "26",
doi = "10.3390/antibiotics8010026"
}

Ašanin, J., Mišić, D., Aksentijević, K., Tambur, Z., Rakonjac, B., Kovačević, I., Spergser, J.,& Loncarić, I.. (2019). Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel
MDPI, Basel., 8(1), 26.
https://doi.org/10.3390/antibiotics8010026

Ašanin J, Mišić D, Aksentijević K, Tambur Z, Rakonjac B, Kovačević I, Spergser J, Loncarić I. Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel. 2019;8(1):26.
doi:10.3390/antibiotics8010026 .

Ašanin, Jelena, Mišić, Dušan, Aksentijević, Ksenija, Tambur, Zoran, Rakonjac, Bojan, Kovačević, Ivana, Spergser, Joachim, Loncarić, Igor, "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia" in Antibiotics-Basel, 8, no. 1 (2019):26,
https://doi.org/10.3390/antibiotics8010026 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Ašanin, Jelena
AU  - Nunney, Tim
AU  - Radovanović, Željko
AU  - Radoičić, Marija
AU  - Mitrić, Miodrag
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1773
AB  - This study discusses the possibility of fabrication of textile nanocomposites with antimicrobial activity against antibiotics-resistant bacterial strains and yeast. Modification of cotton fabric with oxalic acid solutions of different concentrations provided free carboxyl groups for binding of Cu2+ -ions from copper (II) sulfate solution which were further reduced with sodium borohydride in alkaline solution. An increase in the concentration of applied oxalic acid resulted in larger amounts of free carboxyl groups on the cotton fibers, Cu2+ -ions uptake and total amounts of Cu-based nanoparticles after reduction. XPS and XRD analyses suggested that nanoparticles mainly consisted of CuO with fractions of Cu2O. Fabricated textile nanocomposites ensured maximum reduction of Gram-negative E. coli ATCC 25922, E. coli NCTC 13846, E. coli ATCC BAA-2469, K. pneumoniae ATCC-BAA 2146 and P. aeruginosa ATCC 27853, Gram-positive bacteria S. aureus ATCC 25923 and S. aureus ATCC 43300 and yeast C. albicans ATCC 24433. Additionally, controlled release of Cu2+ -ions from fabrics into the physiological saline solution was obtained within 24 hours.
PB  - Korean Fiber Soc, Seoul
T2  - Fibers and Polymers
T1  - Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles
VL  - 20
IS  - 11
SP  - 2317
EP  - 2325
DO  - 10.1007/s12221-019-9131-5
ER  - 

@article{
author = "Marković, Darka and Ašanin, Jelena and Nunney, Tim and Radovanović, Željko and Radoičić, Marija and Mitrić, Miodrag and Mišić, Dušan and Radetić, Maja",
year = "2019",
abstract = "This study discusses the possibility of fabrication of textile nanocomposites with antimicrobial activity against antibiotics-resistant bacterial strains and yeast. Modification of cotton fabric with oxalic acid solutions of different concentrations provided free carboxyl groups for binding of Cu2+ -ions from copper (II) sulfate solution which were further reduced with sodium borohydride in alkaline solution. An increase in the concentration of applied oxalic acid resulted in larger amounts of free carboxyl groups on the cotton fibers, Cu2+ -ions uptake and total amounts of Cu-based nanoparticles after reduction. XPS and XRD analyses suggested that nanoparticles mainly consisted of CuO with fractions of Cu2O. Fabricated textile nanocomposites ensured maximum reduction of Gram-negative E. coli ATCC 25922, E. coli NCTC 13846, E. coli ATCC BAA-2469, K. pneumoniae ATCC-BAA 2146 and P. aeruginosa ATCC 27853, Gram-positive bacteria S. aureus ATCC 25923 and S. aureus ATCC 43300 and yeast C. albicans ATCC 24433. Additionally, controlled release of Cu2+ -ions from fabrics into the physiological saline solution was obtained within 24 hours.",
publisher = "Korean Fiber Soc, Seoul",
journal = "Fibers and Polymers",
title = "Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles",
volume = "20",
number = "11",
pages = "2317-2325",
doi = "10.1007/s12221-019-9131-5"
}

Marković, D., Ašanin, J., Nunney, T., Radovanović, Ž., Radoičić, M., Mitrić, M., Mišić, D.,& Radetić, M.. (2019). Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles. in Fibers and Polymers
Korean Fiber Soc, Seoul., 20(11), 2317-2325.
https://doi.org/10.1007/s12221-019-9131-5

Marković D, Ašanin J, Nunney T, Radovanović Ž, Radoičić M, Mitrić M, Mišić D, Radetić M. Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles. in Fibers and Polymers. 2019;20(11):2317-2325.
doi:10.1007/s12221-019-9131-5 .

Marković, Darka, Ašanin, Jelena, Nunney, Tim, Radovanović, Željko, Radoičić, Marija, Mitrić, Miodrag, Mišić, Dušan, Radetić, Maja, "Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles" in Fibers and Polymers, 20, no. 11 (2019):2317-2325,
https://doi.org/10.1007/s12221-019-9131-5 . .

TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1775
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Jokić, Bojan
AU  - Radovanović, Željko
AU  - Ašanin, Jelena
AU  - Radoičić, Marija
AU  - Mitrić, Miodrag
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1786
AB  - This study discusses the possibility of in situ synthesis of Cu-based nanoparticles (NPs) on cotton and viscose rayon fabrics previously modified with 1,2,3,4-butanetetracarboxylic acid (BTCA) of different concentrations, with the aim to obtain antibacterial protection. The changes in the chemical structure of the fibers caused by the modification with BTCA were assessed by FTIR spectroscopy. The higher the concentration of the applied BTCA, the larger the amounts of free carboxyl groups in both fabrics. AAS analysis showed that the samples modified with higher concentration of BTCA provided larger Cu2+-ions uptake and consequently, higher content of Cu after Cu2+-ions reduction. The presence of Cu-based NPs on the fabrics was confirmed by FESEM and EDS analyses. XRD analysis revealed that NPs appeared as a mixture of CuO and Cu2O. Synthesized NPs imparted excellent antibacterial activity to both fabrics against E. coli and S. aureus, including methicillin-resistant S. aureus.
PB  - Editura Acad Romane, Bucuresti
T2  - Cellulose Chemistry and Technology
T1  - Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of CuO/Cu2O nanoparticles on cotton and viscose rayon fabrics
VL  - 53
IS  - 7-8
SP  - 805
EP  - 813
DO  - 10.35812/CelluloseChemTechnol.2019.53.79
ER  - 

@article{
author = "Marković, Darka and Jokić, Bojan and Radovanović, Željko and Ašanin, Jelena and Radoičić, Marija and Mitrić, Miodrag and Mišić, Dušan and Radetić, Maja",
year = "2019",
abstract = "This study discusses the possibility of in situ synthesis of Cu-based nanoparticles (NPs) on cotton and viscose rayon fabrics previously modified with 1,2,3,4-butanetetracarboxylic acid (BTCA) of different concentrations, with the aim to obtain antibacterial protection. The changes in the chemical structure of the fibers caused by the modification with BTCA were assessed by FTIR spectroscopy. The higher the concentration of the applied BTCA, the larger the amounts of free carboxyl groups in both fabrics. AAS analysis showed that the samples modified with higher concentration of BTCA provided larger Cu2+-ions uptake and consequently, higher content of Cu after Cu2+-ions reduction. The presence of Cu-based NPs on the fabrics was confirmed by FESEM and EDS analyses. XRD analysis revealed that NPs appeared as a mixture of CuO and Cu2O. Synthesized NPs imparted excellent antibacterial activity to both fabrics against E. coli and S. aureus, including methicillin-resistant S. aureus.",
publisher = "Editura Acad Romane, Bucuresti",
journal = "Cellulose Chemistry and Technology",
title = "Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of CuO/Cu2O nanoparticles on cotton and viscose rayon fabrics",
volume = "53",
number = "7-8",
pages = "805-813",
doi = "10.35812/CelluloseChemTechnol.2019.53.79"
}

Marković, D., Jokić, B., Radovanović, Ž., Ašanin, J., Radoičić, M., Mitrić, M., Mišić, D.,& Radetić, M.. (2019). Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of CuO/Cu2O nanoparticles on cotton and viscose rayon fabrics. in Cellulose Chemistry and Technology
Editura Acad Romane, Bucuresti., 53(7-8), 805-813.
https://doi.org/10.35812/CelluloseChemTechnol.2019.53.79

Marković D, Jokić B, Radovanović Ž, Ašanin J, Radoičić M, Mitrić M, Mišić D, Radetić M. Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of CuO/Cu2O nanoparticles on cotton and viscose rayon fabrics. in Cellulose Chemistry and Technology. 2019;53(7-8):805-813.
doi:10.35812/CelluloseChemTechnol.2019.53.79 .

Marković, Darka, Jokić, Bojan, Radovanović, Željko, Ašanin, Jelena, Radoičić, Marija, Mitrić, Miodrag, Mišić, Dušan, Radetić, Maja, "Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of CuO/Cu2O nanoparticles on cotton and viscose rayon fabrics" in Cellulose Chemistry and Technology, 53, no. 7-8 (2019):805-813,
https://doi.org/10.35812/CelluloseChemTechnol.2019.53.79 . .

TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1845
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Bogunović, Danica
AU  - Stević, Nataša
AU  - Sidi-Boumedine, Karim
AU  - Mišić, Dušan
AU  - Tomanović, Snežana
AU  - Kulišić, Zoran
AU  - Magaš, Vladimir
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1589
AB  - Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)
VL  - 68
IS  - 3
SP  - 257
EP  - 268
DO  - 10.2478/acve-2018-0023
ER  - 

@article{
author = "Bogunović, Danica and Stević, Nataša and Sidi-Boumedine, Karim and Mišić, Dušan and Tomanović, Snežana and Kulišić, Zoran and Magaš, Vladimir and Radojičić, Sonja",
year = "2018",
abstract = "Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)",
volume = "68",
number = "3",
pages = "257-268",
doi = "10.2478/acve-2018-0023"
}

Bogunović, D., Stević, N., Sidi-Boumedine, K., Mišić, D., Tomanović, S., Kulišić, Z., Magaš, V.,& Radojičić, S.. (2018). Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 257-268.
https://doi.org/10.2478/acve-2018-0023

Bogunović D, Stević N, Sidi-Boumedine K, Mišić D, Tomanović S, Kulišić Z, Magaš V, Radojičić S. Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd. 2018;68(3):257-268.
doi:10.2478/acve-2018-0023 .

Bogunović, Danica, Stević, Nataša, Sidi-Boumedine, Karim, Mišić, Dušan, Tomanović, Snežana, Kulišić, Zoran, Magaš, Vladimir, Radojičić, Sonja, "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)" in Acta Veterinaria-Beograd, 68, no. 3 (2018):257-268,
https://doi.org/10.2478/acve-2018-0023 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Kalagasidis-Krusić, Melina
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1591
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
VL  - 140
SP  - 11
EP  - 20
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Kalagasidis-Krusić, Melina and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
volume = "140",
pages = "11-20",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Kalagasidis-Krusić, M., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Kalagasidis-Krusić M, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Kalagasidis-Krusić, Melina, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Spergser, Joachim
AU  - Szostak, Michael
AU  - Loncarić, Igor
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1671
PB  - Amer Soc Microbiology, Washington
T2  - Antimicrobial Agents and Chemotherapy
T1  - OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia
VL  - 62
IS  - 7
SP  - e00219-18
DO  - 10.1128/AAC.00219-18
ER  - 

@article{
author = "Mišić, Dušan and Ašanin, Jelena and Spergser, Joachim and Szostak, Michael and Loncarić, Igor",
year = "2018",
publisher = "Amer Soc Microbiology, Washington",
journal = "Antimicrobial Agents and Chemotherapy",
title = "OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia",
volume = "62",
number = "7",
pages = "e00219-18",
doi = "10.1128/AAC.00219-18"
}

Mišić, D., Ašanin, J., Spergser, J., Szostak, M.,& Loncarić, I.. (2018). OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia. in Antimicrobial Agents and Chemotherapy
Amer Soc Microbiology, Washington., 62(7), e00219-18.
https://doi.org/10.1128/AAC.00219-18

Mišić D, Ašanin J, Spergser J, Szostak M, Loncarić I. OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia. in Antimicrobial Agents and Chemotherapy. 2018;62(7):e00219-18.
doi:10.1128/AAC.00219-18 .

Mišić, Dušan, Ašanin, Jelena, Spergser, Joachim, Szostak, Michael, Loncarić, Igor, "OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia" in Antimicrobial Agents and Chemotherapy, 62, no. 7 (2018):e00219-18,
https://doi.org/10.1128/AAC.00219-18 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Krušić, Melina Kalagasidis
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1847
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 °C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier
T2  - The Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
VL  - 140
SP  - 11
EP  - 20
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Krušić, Melina Kalagasidis and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 °C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier",
journal = "The Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
volume = "140",
pages = "11-20",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Krušić, M. K., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in The Journal of Supercritical Fluids
Elsevier., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Krušić MK, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in The Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Krušić, Melina Kalagasidis, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in The Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Stević, Nataša
AU  - Mišić, Dušan
AU  - Bogunović, Danica
AU  - Matović, Kazimir
AU  - Valčić, Miroslav
AU  - Milovanović, Milovan
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1544
AB  - The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay
VL  - 67
IS  - 4
SP  - 551
EP  - 561
DO  - 10.1515/acve-2017-0046
ER  - 

@article{
author = "Stević, Nataša and Mišić, Dušan and Bogunović, Danica and Matović, Kazimir and Valčić, Miroslav and Milovanović, Milovan and Radojičić, Sonja",
year = "2017",
abstract = "The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay",
volume = "67",
number = "4",
pages = "551-561",
doi = "10.1515/acve-2017-0046"
}

Stević, N., Mišić, D., Bogunović, D., Matović, K., Valčić, M., Milovanović, M.,& Radojičić, S.. (2017). Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 551-561.
https://doi.org/10.1515/acve-2017-0046

Stević N, Mišić D, Bogunović D, Matović K, Valčić M, Milovanović M, Radojičić S. Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd. 2017;67(4):551-561.
doi:10.1515/acve-2017-0046 .

Stević, Nataša, Mišić, Dušan, Bogunović, Danica, Matović, Kazimir, Valčić, Miroslav, Milovanović, Milovan, Radojičić, Sonja, "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay" in Acta Veterinaria-Beograd, 67, no. 4 (2017):551-561,
https://doi.org/10.1515/acve-2017-0046 . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Nišavić, Jakov
AU  - Marković, Maja
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1441
AB  - Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL.
AB  - Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibiotic resistance in bacteria isolated from fish in Serbia
T1  - Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije
VL  - 71
IS  - 1
SP  - 24
EP  - 34
DO  - 10.2298/VETGL170329005A
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan",
year = "2017",
abstract = "Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL., Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibiotic resistance in bacteria isolated from fish in Serbia, Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije",
volume = "71",
number = "1",
pages = "24-34",
doi = "10.2298/VETGL170329005A"
}

Aksentijević, K., Ašanin, J., Nišavić, J., Marković, M., Milanov, D.,& Mišić, D.. (2017). Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 24-34.
https://doi.org/10.2298/VETGL170329005A

Aksentijević K, Ašanin J, Nišavić J, Marković M, Milanov D, Mišić D. Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik. 2017;71(1):24-34.
doi:10.2298/VETGL170329005A .

Aksentijević, Ksenija, Ašanin, Jelena, Nišavić, Jakov, Marković, Maja, Milanov, Dubravka, Mišić, Dušan, "Antibiotic resistance in bacteria isolated from fish in Serbia" in Veterinarski Glasnik, 71, no. 1 (2017):24-34,
https://doi.org/10.2298/VETGL170329005A . .

TY  - CHAP
AU  - Milovanović, S.
AU  - Radetić, Maja
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Leontijević, V.
AU  - Ivanović, J.
AU  - Žižović, Irena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1463
AB  - The use of cotton in wound dressing applications is discussed in this chapter. Cotton gauze provides flexibility, controlled adherence to the surrounding tissue, gas permeability, durability and absorbance of fluids exuded from the wound but doesn't provide therapeutic treatment. Therapeutic effect can be provided by incorporation of an active ingredient into gauze. In this chapter, supercritical solvent impregnation of cotton gauze with carvacrol is proposed with an aim to impart antibacterial properties to cotton fibres. State of the art in fabrication of textiles with natural bioactive substances, antibacterial activity of carvacrol loaded materials as well as in the use of supercritical fluids in textile material impregnation is presented. The importance of the application of natural substances with strong antibacterial properties in modern age when the world is facing problem of bacterial resistance to antibiotics is stressed and the future application of cotton as a wound dressing is discussed.
PB  - Nova Science Publishers, Inc.
T2  - Cotton Fibres: Characteristics, Uses and Performance
T1  - High pressure modified cotton in wound dressing applications
SP  - 177
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1463
ER  - 

@inbook{
author = "Milovanović, S. and Radetić, Maja and Mišić, Dušan and Ašanin, Jelena and Leontijević, V. and Ivanović, J. and Žižović, Irena",
year = "2017",
abstract = "The use of cotton in wound dressing applications is discussed in this chapter. Cotton gauze provides flexibility, controlled adherence to the surrounding tissue, gas permeability, durability and absorbance of fluids exuded from the wound but doesn't provide therapeutic treatment. Therapeutic effect can be provided by incorporation of an active ingredient into gauze. In this chapter, supercritical solvent impregnation of cotton gauze with carvacrol is proposed with an aim to impart antibacterial properties to cotton fibres. State of the art in fabrication of textiles with natural bioactive substances, antibacterial activity of carvacrol loaded materials as well as in the use of supercritical fluids in textile material impregnation is presented. The importance of the application of natural substances with strong antibacterial properties in modern age when the world is facing problem of bacterial resistance to antibiotics is stressed and the future application of cotton as a wound dressing is discussed.",
publisher = "Nova Science Publishers, Inc.",
journal = "Cotton Fibres: Characteristics, Uses and Performance",
booktitle = "High pressure modified cotton in wound dressing applications",
pages = "177-205",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1463"
}

Milovanović, S., Radetić, M., Mišić, D., Ašanin, J., Leontijević, V., Ivanović, J.,& Žižović, I.. (2017). High pressure modified cotton in wound dressing applications. in Cotton Fibres: Characteristics, Uses and Performance
Nova Science Publishers, Inc.., 177-205.
https://hdl.handle.net/21.15107/rcub_veterinar_1463

Milovanović S, Radetić M, Mišić D, Ašanin J, Leontijević V, Ivanović J, Žižović I. High pressure modified cotton in wound dressing applications. in Cotton Fibres: Characteristics, Uses and Performance. 2017;:177-205.
https://hdl.handle.net/21.15107/rcub_veterinar_1463 .

Milovanović, S., Radetić, Maja, Mišić, Dušan, Ašanin, Jelena, Leontijević, V., Ivanović, J., Žižović, Irena, "High pressure modified cotton in wound dressing applications" in Cotton Fibres: Characteristics, Uses and Performance (2017):177-205,
https://hdl.handle.net/21.15107/rcub_veterinar_1463 .

TY  - JOUR
AU  - Kiskároly, Ferenc
AU  - Morić, Ivana
AU  - Dokić, Lidija
AU  - Vasiljević, Branka
AU  - Senerović, Lidija
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1480
AB  - The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Development of PCR-based identification of salmonella enterica serovars
VL  - 67
IS  - 2
SP  - 277
EP  - 290
DO  - 10.1515/acve-2017-0022
ER  - 

@article{
author = "Kiskároly, Ferenc and Morić, Ivana and Dokić, Lidija and Vasiljević, Branka and Senerović, Lidija and Mišić, Dušan",
year = "2017",
abstract = "The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Development of PCR-based identification of salmonella enterica serovars",
volume = "67",
number = "2",
pages = "277-290",
doi = "10.1515/acve-2017-0022"
}

Kiskároly, F., Morić, I., Dokić, L., Vasiljević, B., Senerović, L.,& Mišić, D.. (2017). Development of PCR-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 277-290.
https://doi.org/10.1515/acve-2017-0022

Kiskároly F, Morić I, Dokić L, Vasiljević B, Senerović L, Mišić D. Development of PCR-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd. 2017;67(2):277-290.
doi:10.1515/acve-2017-0022 .

Kiskároly, Ferenc, Morić, Ivana, Dokić, Lidija, Vasiljević, Branka, Senerović, Lidija, Mišić, Dušan, "Development of PCR-based identification of salmonella enterica serovars" in Acta Veterinaria-Beograd, 67, no. 2 (2017):277-290,
https://doi.org/10.1515/acve-2017-0022 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1488
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
SP  - 8762649
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
pages = "8762649",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 8762649.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;:8762649.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science (2017):8762649,
https://doi.org/10.1155/2017/8762649 . .

TY  - JOUR
AU  - Bulajić, Snežana
AU  - Čolović, Svetlana
AU  - Mišić, Dušan
AU  - Đorđević, Jasna
AU  - Savić Radovanović, Radoslava
AU  - Ašanin, Jelena
AU  - Ledina, Tijana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1542
AB  - This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
T1  - Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia
VL  - 52
IS  - 12
SP  - 864
EP  - 870
DO  - 10.1080/03601234.2017.1361764
ER  - 

@article{
author = "Bulajić, Snežana and Čolović, Svetlana and Mišić, Dušan and Đorđević, Jasna and Savić Radovanović, Radoslava and Ašanin, Jelena and Ledina, Tijana",
year = "2017",
abstract = "This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS (R) Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g(-1). Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural",
title = "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia",
volume = "52",
number = "12",
pages = "864-870",
doi = "10.1080/03601234.2017.1361764"
}

Bulajić, S., Čolović, S., Mišić, D., Đorđević, J., Savić Radovanović, R., Ašanin, J.,& Ledina, T.. (2017). Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
Taylor & Francis Inc, Philadelphia., 52(12), 864-870.
https://doi.org/10.1080/03601234.2017.1361764

Bulajić S, Čolović S, Mišić D, Đorđević J, Savić Radovanović R, Ašanin J, Ledina T. Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural. 2017;52(12):864-870.
doi:10.1080/03601234.2017.1361764 .

Bulajić, Snežana, Čolović, Svetlana, Mišić, Dušan, Đorđević, Jasna, Savić Radovanović, Radoslava, Ašanin, Jelena, Ledina, Tijana, "Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia" in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 52, no. 12 (2017):864-870,
https://doi.org/10.1080/03601234.2017.1361764 . .

TY  - JOUR
AU  - Buac, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1403
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
VL  - 106
SP  - 135
EP  - 142
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Buac, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
volume = "106",
pages = "135-142",
doi = "10.1016/j.rvsc.2016.04.002"
}

Buac, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Buac M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Buac, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .

TY  - JOUR
AU  - Rađenović, Milan
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1325
AB  - Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students.
AB  - Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade
T1  - Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu
VL  - 9
IS  - 2
SP  - 17
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1325
ER  - 

@article{
author = "Rađenović, Milan and Ašanin, Jelena and Aksentijević, Ksenija and Mišić, Dušan",
year = "2016",
abstract = "Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students., Rezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade, Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu",
volume = "9",
number = "2",
pages = "17-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1325"
}

Rađenović, M., Ašanin, J., Aksentijević, K.,& Mišić, D.. (2016). Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(2), 17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325

Rađenović M, Ašanin J, Aksentijević K, Mišić D. Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade. in Arhiv veterinarske medicine. 2016;9(2):17-28.
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .

Rađenović, Milan, Ašanin, Jelena, Aksentijević, Ksenija, Mišić, Dušan, "Investigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgrade" in Arhiv veterinarske medicine, 9, no. 2 (2016):17-28,
https://hdl.handle.net/21.15107/rcub_veterinar_1325 .