TY  - CONF
AU  - Prošić, Isidora
AU  - Milčić-Matić, Natalija
AU  - Radalj, Andrea
AU  - Nikšić, Aleksandar
AU  - Ilić, Milica
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3879
AB  - Mетицилин-резистентнe стафилококe (МРС) представљају значајан изазов у лечењу бактеријских инфекција кућних љубимаца и у великој мери ограничавају терапијске могућности. Осим тога, ови сојеви су значајна претња по јавно здравље на глобалном нивоу. Спроведена студија имала је за циљ да истражи молекуларну преваленцију коагулазa-позитивних стафилокока (КоПС) које изазивају инфекције коже и ушију код паса и да идентификује МРС из групе КоПС. Из узорака пореклом од паса са инфекцијом ушију и/или коже пристиглих са Клинике за Дерматологију Факултета ветеринарске медицине изоловано је укупно 79 сојева из рода Staphylococcus. На основу разноликости секвенце nuc гена, применом Multiplex PCR методе детектоване су врсте из групе КоПС. Фенотипска резистенција МРС доказана је методом диск-дифузије и одређивањем вредности минималне инхибиторне концентрације, присуство PBP2a (penicillin-binding protein 2a, енгл.) доказано је применом латекс аглутинације, док је молекуларном детекцијом испитано присуство mecA и mecC гена. Међу 76 КоПС изолата, 50/76 (65,8%) је идентификовано као S. pseudintermedius, 17/76 (22,4%) као S. aureus, 6/76 (7,9%) као S. coagulans и 3/76 (3,9%) %) као S. intermedius. Присуство mecA гена је детектовано у 30% (15/50) изолата S. pseudintermedius и 29,4% (5/17) изолата S. aureus који су класификовани као метицилин-резистентне S. pseudintermedius (МРSP) и метицилин-резистентне S. аureus (MRSA). MecC ген није детектован код ни једног изолата. Представљени налази наглашавају високу преваленцију МРС-а изолованих из ушију и коже паса на територији Београда, истичући важност континуираног надзора као првог корака у борби против МРС-а и контролних мера за смањење ширења антимикробне резистенције (АМР). Овакве и сличне студије су важне у решавању проблема АМР и обезбеђивању ефикасних стратегија лечења како животиња тако и људи, имајући у виду све већи број студија које поред MRSA, детектују сојеве S. pseudintermedius и МRSP као опортунистичке патогене за људе и узрочнике емергентних зооноза.
AB  - Methicillin-resistant staphylococci (MRS) limit therapeutic options and pose a significant challenge in the treatment of bacterial infections in companion animals. Moreover, these strains pose a significant threat to global public health. This study aimed to investigate the molecular prevalence of coagulase-positive staphylococci (CoPS) causing skin and ear infections in dogs and to identify methicillin-resistant CoPS. A total of 79 staphylococcal strains were isolated from clinical samples collected from dogs with suspected skin and ear infections from the Dermatology clinic at the Faculty of Veterinary Medicine. Multiplex PCR was employed for species-specific identification of CoPS based on the sequence diversity of the nuc gene. Phenotypic characterization of methicilin resistance was performed using disc-diffusion and determination of minimally inhibitory concentrations values, while PBP2a (penicillin-binding protein 2a) was detected using latex agglutination and finally molecular detection of mecA and mecC genes was performed. Among the 76 CoPS isolates, 50/76 (65.8%) were identified as S. pseudintermedius, 17/76 (22.4%) as S. aureus, 6/76 (7.9%) as S. coagulans, and 3/76 (3.9%) as S. intermedius. The mecA gene was detected in 30% (15/50) of S. pseudintermedius isolates and 29.4% (5/17) of S. aureus isolates, classified as methicillin-resistant S. pseudintermedius (MRSP) and methicillin-resistant S. aureus (MRSA), respectively. No isolates tested positive for the mecC gene. These findings underscore the high prevalence of MRS in canine dermatological infections, highlighting the importance of continuous monitoring as the first step in combating the high prevalence of MRS and robust infection control measures to mitigate the spread of antimicrobial resistance (AMR). Such efforts are crucial in addressing the public health implications associated with AMR and ensuring effective treatment strategies for both humans and animals considering that besides MRSA, newer studies recognize S. pseudintermedius and MRSP strains as opportunistic human pathogens and emerging zoonotic agents.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa
T1  - Molecular prevalence of methicillin-resistant coagulase-positive staphylococci isolated from  canine skin and ears
SP  - 182
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3879
ER  - 

@conference{
author = "Prošić, Isidora and Milčić-Matić, Natalija and Radalj, Andrea and Nikšić, Aleksandar and Ilić, Milica and Krnjaić, Dejan",
year = "2024",
abstract = "Mетицилин-резистентнe стафилококe (МРС) представљају значајан изазов у лечењу бактеријских инфекција кућних љубимаца и у великој мери ограничавају терапијске могућности. Осим тога, ови сојеви су значајна претња по јавно здравље на глобалном нивоу. Спроведена студија имала је за циљ да истражи молекуларну преваленцију коагулазa-позитивних стафилокока (КоПС) које изазивају инфекције коже и ушију код паса и да идентификује МРС из групе КоПС. Из узорака пореклом од паса са инфекцијом ушију и/или коже пристиглих са Клинике за Дерматологију Факултета ветеринарске медицине изоловано је укупно 79 сојева из рода Staphylococcus. На основу разноликости секвенце nuc гена, применом Multiplex PCR методе детектоване су врсте из групе КоПС. Фенотипска резистенција МРС доказана је методом диск-дифузије и одређивањем вредности минималне инхибиторне концентрације, присуство PBP2a (penicillin-binding protein 2a, енгл.) доказано је применом латекс аглутинације, док је молекуларном детекцијом испитано присуство mecA и mecC гена. Међу 76 КоПС изолата, 50/76 (65,8%) је идентификовано као S. pseudintermedius, 17/76 (22,4%) као S. aureus, 6/76 (7,9%) као S. coagulans и 3/76 (3,9%) %) као S. intermedius. Присуство mecA гена је детектовано у 30% (15/50) изолата S. pseudintermedius и 29,4% (5/17) изолата S. aureus који су класификовани као метицилин-резистентне S. pseudintermedius (МРSP) и метицилин-резистентне S. аureus (MRSA). MecC ген није детектован код ни једног изолата. Представљени налази наглашавају високу преваленцију МРС-а изолованих из ушију и коже паса на територији Београда, истичући важност континуираног надзора као првог корака у борби против МРС-а и контролних мера за смањење ширења антимикробне резистенције (АМР). Овакве и сличне студије су важне у решавању проблема АМР и обезбеђивању ефикасних стратегија лечења како животиња тако и људи, имајући у виду све већи број студија које поред MRSA, детектују сојеве S. pseudintermedius и МRSP као опортунистичке патогене за људе и узрочнике емергентних зооноза., Methicillin-resistant staphylococci (MRS) limit therapeutic options and pose a significant challenge in the treatment of bacterial infections in companion animals. Moreover, these strains pose a significant threat to global public health. This study aimed to investigate the molecular prevalence of coagulase-positive staphylococci (CoPS) causing skin and ear infections in dogs and to identify methicillin-resistant CoPS. A total of 79 staphylococcal strains were isolated from clinical samples collected from dogs with suspected skin and ear infections from the Dermatology clinic at the Faculty of Veterinary Medicine. Multiplex PCR was employed for species-specific identification of CoPS based on the sequence diversity of the nuc gene. Phenotypic characterization of methicilin resistance was performed using disc-diffusion and determination of minimally inhibitory concentrations values, while PBP2a (penicillin-binding protein 2a) was detected using latex agglutination and finally molecular detection of mecA and mecC genes was performed. Among the 76 CoPS isolates, 50/76 (65.8%) were identified as S. pseudintermedius, 17/76 (22.4%) as S. aureus, 6/76 (7.9%) as S. coagulans, and 3/76 (3.9%) as S. intermedius. The mecA gene was detected in 30% (15/50) of S. pseudintermedius isolates and 29.4% (5/17) of S. aureus isolates, classified as methicillin-resistant S. pseudintermedius (MRSP) and methicillin-resistant S. aureus (MRSA), respectively. No isolates tested positive for the mecC gene. These findings underscore the high prevalence of MRS in canine dermatological infections, highlighting the importance of continuous monitoring as the first step in combating the high prevalence of MRS and robust infection control measures to mitigate the spread of antimicrobial resistance (AMR). Such efforts are crucial in addressing the public health implications associated with AMR and ensuring effective treatment strategies for both humans and animals considering that besides MRSA, newer studies recognize S. pseudintermedius and MRSP strains as opportunistic human pathogens and emerging zoonotic agents.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa, Molecular prevalence of methicillin-resistant coagulase-positive staphylococci isolated from  canine skin and ears",
pages = "182-183",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3879"
}

Prošić, I., Milčić-Matić, N., Radalj, A., Nikšić, A., Ilić, M.,& Krnjaić, D.. (2024). Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3879

Prošić I, Milčić-Matić N, Radalj A, Nikšić A, Ilić M, Krnjaić D. Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3879 .

Prošić, Isidora, Milčić-Matić, Natalija, Radalj, Andrea, Nikšić, Aleksandar, Ilić, Milica, Krnjaić, Dejan, "Molekularna prevalencija meticilin-rezistentnih koagulaza-pozitivnih stafilokoka izolovanih iz kože i ušiju pasa" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):182-183,
https://hdl.handle.net/21.15107/rcub_veterinar_3879 .

TY  - CONF
AU  - Radalj, Andrea
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Prošić, Isidora
AU  - Ilić, Milica
AU  - Nikšić, Aleksandar
AU  - Nišavić, Jakov
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3721
AB  - Lančana reakcija polimeraze (PCR) je u današnje vreme jedna
od najčešće korišćenih metoda u kliničkoj mikrobiologiji pri čemu naročito
olakšava dijagnostiku virusnih infekcija. Adenovirusi pasa 1 i 2
(CaDV1 i 2) su srodni dvolančani DNK virusi bez spoljašnjeg omotača
i predstavnici familije Adenoviridae koji ispoljavaju različit tropizam.
CaDV1 se umnožava u ćelijama endotela krvnih sudova kao i parenhima
jetre i bubrega, dok se CaDV2 vezuje za receptore ćelija respiratornog
i crevnog epitela. Infektivni hepatitis pasa karakterišu klinički
simptomi akutnog hepatitisa i posledica je infekcije adenovirusom pasa
tip 1. Adenovirus pasa tip 2 izaziva respiratorno oboljenje i jedan
je od patogena u sklopu kompleksa infektivnih respiratornih oboljenja
pasa, odnosno infektivnog traheobronhitisa. Osim pasa, na infekciju
adenovirusima prijemčivi su i divlji mesojedi koji su ujedno i rezervoari
CaDV1 u prirodi. Pojava infektivnog hepatitisa u populaciji pasa je
u današnje vreme retka zahvaljujući sprovođenju postupka vakcinacije.
Međutim, ovo oboljenje je i dalje prisutno kod nevakcinisanih pasa,
odnosno u slučajevima njihovog kontakta sa divljim mesojedima.
PCR se rutinski koristi u dijagnostici adenovirusnih infekcija pasa. Navedena
metoda obezbeđuje brzo dobijanje pouzdanih rezultata ispitivanja
što često nije moguće primenom klasičnih virusoloških metoda,
a naročito u slučajevima ispitivanja autoliziranih uzoraka tkiva divljih
mesojeda. Metoda PCR, uz upotrebu parova prajmera specifičnih za
visokokonzervirane fragmente E3 regiona genoma adenovirusa čijom
se primenom obezbeđuje detekcija i diferencijacija CaDV1 i CaDV2,
će se vršiti ispitivanjem ekstrakata DNK različitih tkiva lisica i šakala.
PCR će se vršiti po uspostavljenom protokolu uz izvođenje horizontalne
gel elektroforeze radi vizualizacije i analize dobijenih PCR produkata.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Primena molekularnih metoda u dijagnostici infekcija izazvanih adenovirusima pasa
SP  - 133
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3721
ER  - 

@conference{
author = "Radalj, Andrea and Milić, Nenad and Krnjaić, Dejan and Prošić, Isidora and Ilić, Milica and Nikšić, Aleksandar and Nišavić, Jakov",
year = "2024",
abstract = "Lančana reakcija polimeraze (PCR) je u današnje vreme jedna
od najčešće korišćenih metoda u kliničkoj mikrobiologiji pri čemu naročito
olakšava dijagnostiku virusnih infekcija. Adenovirusi pasa 1 i 2
(CaDV1 i 2) su srodni dvolančani DNK virusi bez spoljašnjeg omotača
i predstavnici familije Adenoviridae koji ispoljavaju različit tropizam.
CaDV1 se umnožava u ćelijama endotela krvnih sudova kao i parenhima
jetre i bubrega, dok se CaDV2 vezuje za receptore ćelija respiratornog
i crevnog epitela. Infektivni hepatitis pasa karakterišu klinički
simptomi akutnog hepatitisa i posledica je infekcije adenovirusom pasa
tip 1. Adenovirus pasa tip 2 izaziva respiratorno oboljenje i jedan
je od patogena u sklopu kompleksa infektivnih respiratornih oboljenja
pasa, odnosno infektivnog traheobronhitisa. Osim pasa, na infekciju
adenovirusima prijemčivi su i divlji mesojedi koji su ujedno i rezervoari
CaDV1 u prirodi. Pojava infektivnog hepatitisa u populaciji pasa je
u današnje vreme retka zahvaljujući sprovođenju postupka vakcinacije.
Međutim, ovo oboljenje je i dalje prisutno kod nevakcinisanih pasa,
odnosno u slučajevima njihovog kontakta sa divljim mesojedima.
PCR se rutinski koristi u dijagnostici adenovirusnih infekcija pasa. Navedena
metoda obezbeđuje brzo dobijanje pouzdanih rezultata ispitivanja
što često nije moguće primenom klasičnih virusoloških metoda,
a naročito u slučajevima ispitivanja autoliziranih uzoraka tkiva divljih
mesojeda. Metoda PCR, uz upotrebu parova prajmera specifičnih za
visokokonzervirane fragmente E3 regiona genoma adenovirusa čijom
se primenom obezbeđuje detekcija i diferencijacija CaDV1 i CaDV2,
će se vršiti ispitivanjem ekstrakata DNK različitih tkiva lisica i šakala.
PCR će se vršiti po uspostavljenom protokolu uz izvođenje horizontalne
gel elektroforeze radi vizualizacije i analize dobijenih PCR produkata.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Primena molekularnih metoda u dijagnostici infekcija izazvanih adenovirusima pasa",
pages = "133-140",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3721"
}

Radalj, A., Milić, N., Krnjaić, D., Prošić, I., Ilić, M., Nikšić, A.,& Nišavić, J.. (2024). Primena molekularnih metoda u dijagnostici infekcija izazvanih adenovirusima pasa. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 133-140.
https://hdl.handle.net/21.15107/rcub_veterinar_3721

Radalj A, Milić N, Krnjaić D, Prošić I, Ilić M, Nikšić A, Nišavić J. Primena molekularnih metoda u dijagnostici infekcija izazvanih adenovirusima pasa. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:133-140.
https://hdl.handle.net/21.15107/rcub_veterinar_3721 .

Radalj, Andrea, Milić, Nenad, Krnjaić, Dejan, Prošić, Isidora, Ilić, Milica, Nikšić, Aleksandar, Nišavić, Jakov, "Primena molekularnih metoda u dijagnostici infekcija izazvanih adenovirusima pasa" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):133-140,
https://hdl.handle.net/21.15107/rcub_veterinar_3721 .

TY  - JOUR
AU  - Prošić, Isidora
AU  - Milčić Matić, Natalija
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Aksentijević, Ksenija
AU  - Ilić, Milica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Gajdov, Vladimir
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3763
AB  - The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study
VL  - 74
IS  - 1
SP  - 117
EP  - 132
DO  - 10.2478/acve-2024-0009
DO  - 10.2478/acve-2024-0009
ER  - 

@article{
author = "Prošić, Isidora and Milčić Matić, Natalija and Milić, Nenad and Radalj, Andrea and Aksentijević, Ksenija and Ilić, Milica and Nišavić, Jakov and Radojičić, Marina and Gajdov, Vladimir and Krnjaić, Dejan",
year = "2024",
abstract = "The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study",
volume = "74",
number = "1",
pages = "117-132",
doi = "10.2478/acve-2024-0009, 10.2478/acve-2024-0009"
}

Prošić, I., Milčić Matić, N., Milić, N., Radalj, A., Aksentijević, K., Ilić, M., Nišavić, J., Radojičić, M., Gajdov, V.,& Krnjaić, D.. (2024). Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 117-132.
https://doi.org/10.2478/acve-2024-0009

Prošić I, Milčić Matić N, Milić N, Radalj A, Aksentijević K, Ilić M, Nišavić J, Radojičić M, Gajdov V, Krnjaić D. Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria. 2024;74(1):117-132.
doi:10.2478/acve-2024-0009 .

Prošić, Isidora, Milčić Matić, Natalija, Milić, Nenad, Radalj, Andrea, Aksentijević, Ksenija, Ilić, Milica, Nišavić, Jakov, Radojičić, Marina, Gajdov, Vladimir, Krnjaić, Dejan, "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study" in Acta Veterinaria, 74, no. 1 (2024):117-132,
https://doi.org/10.2478/acve-2024-0009 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3702
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
VL  - 48
IS  - 1
SP  - 591
EP  - 596
DO  - 10.1007/s11259-023-10224-9
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2024",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
volume = "48",
number = "1",
pages = "591-596",
doi = "10.1007/s11259-023-10224-9"
}

Spasojević, D., Zmejkoski, D., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2024). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications
Springer., 48(1), 591-596.
https://doi.org/10.1007/s11259-023-10224-9

Spasojević D, Zmejkoski D, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2024;48(1):591-596.
doi:10.1007/s11259-023-10224-9 .

Spasojević, Dragica, Zmejkoski, Danica, Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications, 48, no. 1 (2024):591-596,
https://doi.org/10.1007/s11259-023-10224-9 . .

TY  - CONF
AU  - Krnjaić, Dejan
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
AU  - Nešić, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3302
PB  - Beograd : Fakultet veterinarske medicine
C3  - 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
T1  - Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup
SP  - 10
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3302
ER  - 

@conference{
author = "Krnjaić, Dejan and Marjanović, Đorđe and Trailović, Saša and Nešić, Vladimir",
year = "2023",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023",
title = "Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup",
pages = "10-13",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3302"
}

Krnjaić, D., Marjanović, Đ., Trailović, S.,& Nešić, V.. (2023). Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
Beograd : Fakultet veterinarske medicine., 10-13.
https://hdl.handle.net/21.15107/rcub_veterinar_3302

Krnjaić D, Marjanović Đ, Trailović S, Nešić V. Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023. 2023;:10-13.
https://hdl.handle.net/21.15107/rcub_veterinar_3302 .

Krnjaić, Dejan, Marjanović, Đorđe, Trailović, Saša, Nešić, Vladimir, "Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup" in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023 (2023):10-13,
https://hdl.handle.net/21.15107/rcub_veterinar_3302 .

TY  - CONF
AU  - Krnjaić, Dejan
AU  - Đorđević, Milutin
AU  - Radalj, Andrea
AU  - Glišić, Dimitrije
AU  - Nišavić, Jakov
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3211
AB  - Od pojave afričke kuge svinja (AKS) u Gruziji 2007. godine, virus genotipa II
proširio se u mnoge države sveta, pre svega Evrope i Azije, dovodeći do ogromnih
zdravstvenih problema i ekonomskih gubitaka u svinjarstvu. Divlje svinje imaju važnu
ulogu u održavanju virusa AKS u prirodi, a ciklus kruženja virusa kod divljih svinja se
može odvijati bez domaćih svinja ili potencijalnih vektora krpelja, sa mogućnošću
prelivanja bolesti u populaciju domaćih svinja. Izbor mera prevencije pojave, širenja i
suzbijanja AKS kod divljih svinja zavisi od načina unošenja bolesti, preko zaraženih
divljih svinja ili indirektno ljudskim aktivnostima, kao i od same faze dinamike
infekcije na nivou populacije. Kontrola AKS je komplikovanija i teža u slučaju
enzootskog prisustva bolesti. Pojačan pasivni nadzor u lovištima predstavlja meru
koju je potrebno sprovesti što je pre moguće. Uklanjanje leševa potencijalno zaraženih
divljih svinja u kojima virus može da ostane infektivan mesecima snižava rizik od
pojave i širenja bolesti. Neophodno je sprovoditi plan stalne pretrage i nadzora delova
lovišta u kojima se nalaze prirodna staništa divljih svinja radi pronalaženja i
uklanjanja leševa. Dodatne mere uključuju redukciju populacije divljih svinja kao i
ograničavanje njihovog kretanja podizanjem ograda. Organizovanje intezivnog
pojedinačnog i grupnog lova divljih svinja u cilju brzog smanjenje brojnosti obuhvata
naročito starosnu kategoriju zrelih ženki. Sistematskim i kontinuiranim odstrelom
može se uspostaviti i održavati linija sprečavanja širenja AKS u područjima visokog
rizika odnosno u zaštitnim područjima intezivnog lova. Podizanje ograda ili sistema
višestrukih ograda može doprineti zaštiti regiona slobodnih od AKS. Neophodno je
sprovoditi aktivnosti usmerene na podizanje pozornosti svih zainteresovanih strana,
pre svega lovaca i držaoca domaćih svinja. Da bi se smanjio rizik od prodora virusa na
poljoprivredna gazdinstva i farme treba doneti i implementirati propise koje
propisuju obaveznost uvođenja mera biosigurnosti. Imajući u vidu da afrička kuga svinja predstavlja kontagiozno oboljenje svinja koje je često praćeno i 100%
mortalitetom i protiv koga ne postoji efikasna vakcina, ključno je sprovođenje detaljne
procene rizika, donošenje i primena odgovarajućih mera prevencije i suzbijanja
bolesti, kao i transparentna komunikacija o pojavi i širenju ove bolesti koja može u
potpunosti da uništi proizvodnju svinja i da ugrozi prehrambenu sigurnost u Republici
Srbiji.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Prevencija širenja i suzbijanja afričke kuge svinja kod divljih svinja
SP  - 199
EP  - 215
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3211
ER  - 

@conference{
author = "Krnjaić, Dejan and Đorđević, Milutin and Radalj, Andrea and Glišić, Dimitrije and Nišavić, Jakov",
year = "2023",
abstract = "Od pojave afričke kuge svinja (AKS) u Gruziji 2007. godine, virus genotipa II
proširio se u mnoge države sveta, pre svega Evrope i Azije, dovodeći do ogromnih
zdravstvenih problema i ekonomskih gubitaka u svinjarstvu. Divlje svinje imaju važnu
ulogu u održavanju virusa AKS u prirodi, a ciklus kruženja virusa kod divljih svinja se
može odvijati bez domaćih svinja ili potencijalnih vektora krpelja, sa mogućnošću
prelivanja bolesti u populaciju domaćih svinja. Izbor mera prevencije pojave, širenja i
suzbijanja AKS kod divljih svinja zavisi od načina unošenja bolesti, preko zaraženih
divljih svinja ili indirektno ljudskim aktivnostima, kao i od same faze dinamike
infekcije na nivou populacije. Kontrola AKS je komplikovanija i teža u slučaju
enzootskog prisustva bolesti. Pojačan pasivni nadzor u lovištima predstavlja meru
koju je potrebno sprovesti što je pre moguće. Uklanjanje leševa potencijalno zaraženih
divljih svinja u kojima virus može da ostane infektivan mesecima snižava rizik od
pojave i širenja bolesti. Neophodno je sprovoditi plan stalne pretrage i nadzora delova
lovišta u kojima se nalaze prirodna staništa divljih svinja radi pronalaženja i
uklanjanja leševa. Dodatne mere uključuju redukciju populacije divljih svinja kao i
ograničavanje njihovog kretanja podizanjem ograda. Organizovanje intezivnog
pojedinačnog i grupnog lova divljih svinja u cilju brzog smanjenje brojnosti obuhvata
naročito starosnu kategoriju zrelih ženki. Sistematskim i kontinuiranim odstrelom
može se uspostaviti i održavati linija sprečavanja širenja AKS u područjima visokog
rizika odnosno u zaštitnim područjima intezivnog lova. Podizanje ograda ili sistema
višestrukih ograda može doprineti zaštiti regiona slobodnih od AKS. Neophodno je
sprovoditi aktivnosti usmerene na podizanje pozornosti svih zainteresovanih strana,
pre svega lovaca i držaoca domaćih svinja. Da bi se smanjio rizik od prodora virusa na
poljoprivredna gazdinstva i farme treba doneti i implementirati propise koje
propisuju obaveznost uvođenja mera biosigurnosti. Imajući u vidu da afrička kuga svinja predstavlja kontagiozno oboljenje svinja koje je često praćeno i 100%
mortalitetom i protiv koga ne postoji efikasna vakcina, ključno je sprovođenje detaljne
procene rizika, donošenje i primena odgovarajućih mera prevencije i suzbijanja
bolesti, kao i transparentna komunikacija o pojavi i širenju ove bolesti koja može u
potpunosti da uništi proizvodnju svinja i da ugrozi prehrambenu sigurnost u Republici
Srbiji.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Prevencija širenja i suzbijanja afričke kuge svinja kod divljih svinja",
pages = "199-215",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3211"
}

Krnjaić, D., Đorđević, M., Radalj, A., Glišić, D.,& Nišavić, J.. (2023). Prevencija širenja i suzbijanja afričke kuge svinja kod divljih svinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 199-215.
https://hdl.handle.net/21.15107/rcub_veterinar_3211

Krnjaić D, Đorđević M, Radalj A, Glišić D, Nišavić J. Prevencija širenja i suzbijanja afričke kuge svinja kod divljih svinja. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:199-215.
https://hdl.handle.net/21.15107/rcub_veterinar_3211 .

Krnjaić, Dejan, Đorđević, Milutin, Radalj, Andrea, Glišić, Dimitrije, Nišavić, Jakov, "Prevencija širenja i suzbijanja afričke kuge svinja kod divljih svinja" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):199-215,
https://hdl.handle.net/21.15107/rcub_veterinar_3211 .

TY  - CONF
AU  - Đorđević, Milutin
AU  - Cvetković, Ružica
AU  - Drašković, Vladimir
AU  - Pešić, Branislav
AU  - Krnjaić, Dejan
AU  - Janković, Ljiljana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3238
AB  - Lov odavnina predstavlja jedan od izvora hrane, kao i ostalih proizvoda koji se
koriste za potrebe ljudi. Pored trofeja i mesa divljači koji za lovce predstavljaju jedan
od glavnih razloga za lovne aktivnosti, za našu struku posebno važno mesto imaju i
sporedni proizvodi životinjskog porekla koji nastaju u toku lova. Sporedni proizvodi
životinjskog porekla koji nastaju u lovištu obuhvataju sve delove tela odstreljene
divljači, koji nisu namenjeni za ishranu ljudi i definišu se kao nusproizvodi lova. Ovi
proizvodi mogu imati različite namene i upotrebu, ali takođe mogu predstavljati i
potencijalnu opasnost sa epidemiološko-epizotiološkog i ekološkog aspekta, ukoliko
se na adekvatan način neškodiljivo ne uklanjaju. Uvažavajući sve zdravstvene i
ekološke rizike koje donose sporedni proizvodi životinjskog porekla, koji potiču od
odstreljene divljači, veoma je važno da lovni radnici i lovci poštuju sve važeće propise
i zakone koji se odnose na problematiku neškodljivog uklanjanja sporednih proizvoda
životinjskog porekla. U Republici Srbiji je posebnim zakonskim aktima regulisano
sakupljanje, prerada i uništavanje sporednih proizvoda životinjskog porekla. U radu
je analizom dostupnih podataka o određenim vrstama odstreljene divljači u Republici
Srbiji predstavljena potrencijalna količina sporednih proizvoda životinjskog porekla
poreklom iz u lovišta, sa ciljem da se istakne važnost navedene problematike.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Lovišta kao izvor sporednih poizvoda životinjskog porekla
SP  - 226
EP  - 236
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3238
ER  - 

@conference{
author = "Đorđević, Milutin and Cvetković, Ružica and Drašković, Vladimir and Pešić, Branislav and Krnjaić, Dejan and Janković, Ljiljana",
year = "2023",
abstract = "Lov odavnina predstavlja jedan od izvora hrane, kao i ostalih proizvoda koji se
koriste za potrebe ljudi. Pored trofeja i mesa divljači koji za lovce predstavljaju jedan
od glavnih razloga za lovne aktivnosti, za našu struku posebno važno mesto imaju i
sporedni proizvodi životinjskog porekla koji nastaju u toku lova. Sporedni proizvodi
životinjskog porekla koji nastaju u lovištu obuhvataju sve delove tela odstreljene
divljači, koji nisu namenjeni za ishranu ljudi i definišu se kao nusproizvodi lova. Ovi
proizvodi mogu imati različite namene i upotrebu, ali takođe mogu predstavljati i
potencijalnu opasnost sa epidemiološko-epizotiološkog i ekološkog aspekta, ukoliko
se na adekvatan način neškodiljivo ne uklanjaju. Uvažavajući sve zdravstvene i
ekološke rizike koje donose sporedni proizvodi životinjskog porekla, koji potiču od
odstreljene divljači, veoma je važno da lovni radnici i lovci poštuju sve važeće propise
i zakone koji se odnose na problematiku neškodljivog uklanjanja sporednih proizvoda
životinjskog porekla. U Republici Srbiji je posebnim zakonskim aktima regulisano
sakupljanje, prerada i uništavanje sporednih proizvoda životinjskog porekla. U radu
je analizom dostupnih podataka o određenim vrstama odstreljene divljači u Republici
Srbiji predstavljena potrencijalna količina sporednih proizvoda životinjskog porekla
poreklom iz u lovišta, sa ciljem da se istakne važnost navedene problematike.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Lovišta kao izvor sporednih poizvoda životinjskog porekla",
pages = "226-236",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3238"
}

Đorđević, M., Cvetković, R., Drašković, V., Pešić, B., Krnjaić, D.,& Janković, L.. (2023). Lovišta kao izvor sporednih poizvoda životinjskog porekla. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 226-236.
https://hdl.handle.net/21.15107/rcub_veterinar_3238

Đorđević M, Cvetković R, Drašković V, Pešić B, Krnjaić D, Janković L. Lovišta kao izvor sporednih poizvoda životinjskog porekla. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:226-236.
https://hdl.handle.net/21.15107/rcub_veterinar_3238 .

Đorđević, Milutin, Cvetković, Ružica, Drašković, Vladimir, Pešić, Branislav, Krnjaić, Dejan, Janković, Ljiljana, "Lovišta kao izvor sporednih poizvoda životinjskog porekla" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):226-236,
https://hdl.handle.net/21.15107/rcub_veterinar_3238 .

TY  - CONF
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Radalj, Andrea
AU  - Prošić, Isidora
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3097
AB  - Rezistencija bakterija na antibiotike je rastući problem koji je
Svetska zdravstvena organizacija proglasila jednom od najvećih globalnih
pretnji po zdravlje ljudi, pri čemu rezistencija na meticilin kod
Staphylococcus vrsta (MRS) predstavlja jedan od najopasnijih oblika
rezistencije bakterija na antibiotike koja se ispoljava prema svim beta-
laktamima izuzev pete generacije cefalosporina. Meticilin rezistentne
S. aureus (MRSA) su zoonotski agensi koji izazivaju lokalne i sistemske
infekcije i koji usled nedostatka efikasne antibiotske terapije
mogu dovesti do fatalnog ishoda infekcija ljudi i životinja. Monitoring
MRSA je od izuzetnog značaja za razumevanje i predviđanje nastanka
i širenja ovih sojeva i sprovodi se u cilju preduzimanja proaktivnih
mera za sprečavanje daljeg širenja rezistencije. MRSA sojevi u genomu
sadrže mobilne genetičke elemente, tzv. stafilokokni kasetni hromozom
(staphylococcal cassette chromosome – SCC, eng.) sa prisutnim
mecA genom koji kodira sintezu penicillin-vezujućeg proteina
2a (penicillin-binding protein 2a – PBP 2a, eng.). PBP 2a je protein
koji se odlikuje transpeptidazom aktivnošću i predstavlja alternativu
penicillin-vezujućem proteinu 2 (penicillin-binding protein 2 – PBP 2,
eng.) za kojeg se vezuju beta-laktamski antibiotici. Posedovanje pored
PBP 2 i PBP 2a, prema kome beta-laktamskih antibiotika imaju
nizak afinitet vezivanja, čini osnovni mehanizam rezistencije kod MRSA.
Dokazivanje prisustva MRSA vršiće se u kliničkim izolatima poreklom
od obolelih pasa i mačaka i to primenom disk-difuzione metode
kao najčešće korišćene kvalitativne metode za određivanje rezistencije
kod kliničkih izolata bakterija, a zatim i primenom kvantitativne metode
upotrebom E-test traka. Dodatno, MRSA sojevi biće ispitani na
prisustvo PBP 2a brzim kitom koji se zasniva na principu lateks aglutinacije.
Na kraju će MRSA sojevi biti potvrđeni molekularnom detekcijom
gena rezistencije primenom lančane reakcije polimeraze (PCR).
U današnje vreme PCR predstavlja nezaobilaznu proceduru u većini
mikrobioloških laboratorija, a našao je primenu u ispitivanju osetljivosti bakterija na antibiotike dopunjujući klasične metode i pružajući
mogućnost istovremenog ispitivanja prisustva više genetskih determinanti
rezistencije. Metoda PCR u cilju detekcije gena rezistencije će
se vršiti ispitivanjem ekstrahovane DNK S. aureus po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi
vizuelizacije i analize dobijenih PCR produkata.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
T1  - Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)
SP  - 111
EP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3097
ER  - 

@conference{
author = "Krnjaić, Dejan and Radojičić, Marina and Radalj, Andrea and Prošić, Isidora",
year = "2023",
abstract = "Rezistencija bakterija na antibiotike je rastući problem koji je
Svetska zdravstvena organizacija proglasila jednom od najvećih globalnih
pretnji po zdravlje ljudi, pri čemu rezistencija na meticilin kod
Staphylococcus vrsta (MRS) predstavlja jedan od najopasnijih oblika
rezistencije bakterija na antibiotike koja se ispoljava prema svim beta-
laktamima izuzev pete generacije cefalosporina. Meticilin rezistentne
S. aureus (MRSA) su zoonotski agensi koji izazivaju lokalne i sistemske
infekcije i koji usled nedostatka efikasne antibiotske terapije
mogu dovesti do fatalnog ishoda infekcija ljudi i životinja. Monitoring
MRSA je od izuzetnog značaja za razumevanje i predviđanje nastanka
i širenja ovih sojeva i sprovodi se u cilju preduzimanja proaktivnih
mera za sprečavanje daljeg širenja rezistencije. MRSA sojevi u genomu
sadrže mobilne genetičke elemente, tzv. stafilokokni kasetni hromozom
(staphylococcal cassette chromosome – SCC, eng.) sa prisutnim
mecA genom koji kodira sintezu penicillin-vezujućeg proteina
2a (penicillin-binding protein 2a – PBP 2a, eng.). PBP 2a je protein
koji se odlikuje transpeptidazom aktivnošću i predstavlja alternativu
penicillin-vezujućem proteinu 2 (penicillin-binding protein 2 – PBP 2,
eng.) za kojeg se vezuju beta-laktamski antibiotici. Posedovanje pored
PBP 2 i PBP 2a, prema kome beta-laktamskih antibiotika imaju
nizak afinitet vezivanja, čini osnovni mehanizam rezistencije kod MRSA.
Dokazivanje prisustva MRSA vršiće se u kliničkim izolatima poreklom
od obolelih pasa i mačaka i to primenom disk-difuzione metode
kao najčešće korišćene kvalitativne metode za određivanje rezistencije
kod kliničkih izolata bakterija, a zatim i primenom kvantitativne metode
upotrebom E-test traka. Dodatno, MRSA sojevi biće ispitani na
prisustvo PBP 2a brzim kitom koji se zasniva na principu lateks aglutinacije.
Na kraju će MRSA sojevi biti potvrđeni molekularnom detekcijom
gena rezistencije primenom lančane reakcije polimeraze (PCR).
U današnje vreme PCR predstavlja nezaobilaznu proceduru u većini
mikrobioloških laboratorija, a našao je primenu u ispitivanju osetljivosti bakterija na antibiotike dopunjujući klasične metode i pružajući
mogućnost istovremenog ispitivanja prisustva više genetskih determinanti
rezistencije. Metoda PCR u cilju detekcije gena rezistencije će
se vršiti ispitivanjem ekstrahovane DNK S. aureus po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi
vizuelizacije i analize dobijenih PCR produkata.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023",
title = "Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)",
pages = "111-124",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3097"
}

Krnjaić, D., Radojičić, M., Radalj, A.,& Prošić, I.. (2023). Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA). in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 111-124.
https://hdl.handle.net/21.15107/rcub_veterinar_3097

Krnjaić D, Radojičić M, Radalj A, Prošić I. Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA). in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023. 2023;:111-124.
https://hdl.handle.net/21.15107/rcub_veterinar_3097 .

Krnjaić, Dejan, Radojičić, Marina, Radalj, Andrea, Prošić, Isidora, "Konvencionalna i molekularna detekcija meticilin rezistentnih Staphylococcus aureus (MRSA)" in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023 (2023):111-124,
https://hdl.handle.net/21.15107/rcub_veterinar_3097 .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Toplak, Ivan
AU  - Prodanović, Radiša
AU  - Gallardo, Carmina
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2947
AB  - This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution
PB  - Springer
T2  - Veterinary Research Communications
T1  - Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia
VL  - 47
IS  - 4
SP  - 1925
EP  - 1936
DO  - 10.1007/s11259-023-10145-7
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Toplak, Ivan and Prodanović, Radiša and Gallardo, Carmina and Radojičić, Sonja",
year = "2023",
abstract = "This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia",
volume = "47",
number = "4",
pages = "1925-1936",
doi = "10.1007/s11259-023-10145-7"
}

Glišić, D., Milićević, V., Krnjaić, D., Toplak, I., Prodanović, R., Gallardo, C.,& Radojičić, S.. (2023). Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications
Springer., 47(4), 1925-1936.
https://doi.org/10.1007/s11259-023-10145-7

Glišić D, Milićević V, Krnjaić D, Toplak I, Prodanović R, Gallardo C, Radojičić S. Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications. 2023;47(4):1925-1936.
doi:10.1007/s11259-023-10145-7 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Toplak, Ivan, Prodanović, Radiša, Gallardo, Carmina, Radojičić, Sonja, "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia" in Veterinary Research Communications, 47, no. 4 (2023):1925-1936,
https://doi.org/10.1007/s11259-023-10145-7 . .

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Prodanović, Radiša
AU  - Toplak, Ivan
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3208
AB  - Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka.
AB  - African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Genska varijabilnost virusa afričke kuge svinja u Srbiji
T1  - Genetic variability of African Swine Fever virus in Serbia
SP  - 24
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3208
ER  - 

@conference{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Prodanović, Radiša and Toplak, Ivan and Radojičić, Sonja",
year = "2023",
abstract = "Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka., African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Genska varijabilnost virusa afričke kuge svinja u Srbiji, Genetic variability of African Swine Fever virus in Serbia",
pages = "24-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3208"
}

Glišić, D., Milićević, V., Krnjaić, D., Prodanović, R., Toplak, I.,& Radojičić, S.. (2023). Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208

Glišić D, Milićević V, Krnjaić D, Prodanović R, Toplak I, Radojičić S. Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Prodanović, Radiša, Toplak, Ivan, Radojičić, Sonja, "Genska varijabilnost virusa afričke kuge svinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):24-28,
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

TY  - JOUR
AU  - Vujinović, Slobodan
AU  - Graber, Hans Ulrich
AU  - Vićić, Ivan
AU  - Vejnović, Branislav
AU  - Stevanović, Oliver
AU  - Krnjaić, Dejan
AU  - Milivojević, Dušan
AU  - Katić, Vera
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3289
AB  - This study aimed to determine the prevalence of Staphylococcus aureus subclinical mastitis and to genotype the
S. aureus isolates using the 16S–23S rRNA intergenic spacer (RS-PCR) method. In addition, the genes responsible
for adherence, biofilm formation, host evasion, tissue necrosis, methicillin resistance, and enterotoxin production
of S. aureus were investigated. The overall prevalence of S. aureus subclinical mastitis in lactating cows was 5.4%
(95% confidence interval, CI=4.7–6.1%). An increased risk of S. aureus intramammary infection was observed on
small family farms (odds ratio, OR=4.2, 95% CI=2.6–6.6, P < 0.001) and medium-sized farms (OR=3.5, 95%
CI=2.2–5.7, P < 0.001). The RS-PCR analysis revealed 44 genotypes and genotype variants, of which 15 new
genotypes and five new variants were detected within small and medium-sized farms. S. aureus isolates of new
genotypes and genotype variants carried the clfA gene responsible for adherence at a lower frequency (64.8%)
and enterotoxin-producing genes sea (20.4%), seb (14.8%) and sec (14.8%) at a higher frequency than the other
known genotypes (P < 0.001), and were confirmed to carry the sej and sep genes. The spa gene was detected in all
S. aureus isolates, whereas none harbored bap, ser, or tsst-1 genes. Methicillin-resistant strains of S. aureus (MRSA)
were also detected, with a higher prevalence (19.2%) on large farms with more than 50 cows (P < 0.001). Using
molecular techniques as diagnostic tools provides a better understanding of intramammary staphylococcal infections’ occurrence, spread, and eradication.
PB  - Elsevier
T2  - Comparative Immunology, Microbiology and Infectious Diseases
T1  - Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia
VL  - 101
SP  - 102056
DO  - 10.1016/j.cimid.2023.102056
ER  - 

@article{
author = "Vujinović, Slobodan and Graber, Hans Ulrich and Vićić, Ivan and Vejnović, Branislav and Stevanović, Oliver and Krnjaić, Dejan and Milivojević, Dušan and Katić, Vera",
year = "2023",
abstract = "This study aimed to determine the prevalence of Staphylococcus aureus subclinical mastitis and to genotype the
S. aureus isolates using the 16S–23S rRNA intergenic spacer (RS-PCR) method. In addition, the genes responsible
for adherence, biofilm formation, host evasion, tissue necrosis, methicillin resistance, and enterotoxin production
of S. aureus were investigated. The overall prevalence of S. aureus subclinical mastitis in lactating cows was 5.4%
(95% confidence interval, CI=4.7–6.1%). An increased risk of S. aureus intramammary infection was observed on
small family farms (odds ratio, OR=4.2, 95% CI=2.6–6.6, P < 0.001) and medium-sized farms (OR=3.5, 95%
CI=2.2–5.7, P < 0.001). The RS-PCR analysis revealed 44 genotypes and genotype variants, of which 15 new
genotypes and five new variants were detected within small and medium-sized farms. S. aureus isolates of new
genotypes and genotype variants carried the clfA gene responsible for adherence at a lower frequency (64.8%)
and enterotoxin-producing genes sea (20.4%), seb (14.8%) and sec (14.8%) at a higher frequency than the other
known genotypes (P < 0.001), and were confirmed to carry the sej and sep genes. The spa gene was detected in all
S. aureus isolates, whereas none harbored bap, ser, or tsst-1 genes. Methicillin-resistant strains of S. aureus (MRSA)
were also detected, with a higher prevalence (19.2%) on large farms with more than 50 cows (P < 0.001). Using
molecular techniques as diagnostic tools provides a better understanding of intramammary staphylococcal infections’ occurrence, spread, and eradication.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia",
volume = "101",
pages = "102056",
doi = "10.1016/j.cimid.2023.102056"
}

Vujinović, S., Graber, H. U., Vićić, I., Vejnović, B., Stevanović, O., Krnjaić, D., Milivojević, D.,& Katić, V.. (2023). Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia. in Comparative Immunology, Microbiology and Infectious Diseases
Elsevier., 101, 102056.
https://doi.org/10.1016/j.cimid.2023.102056

Vujinović S, Graber HU, Vićić I, Vejnović B, Stevanović O, Krnjaić D, Milivojević D, Katić V. Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia. in Comparative Immunology, Microbiology and Infectious Diseases. 2023;101:102056.
doi:10.1016/j.cimid.2023.102056 .

Vujinović, Slobodan, Graber, Hans Ulrich, Vićić, Ivan, Vejnović, Branislav, Stevanović, Oliver, Krnjaić, Dejan, Milivojević, Dušan, Katić, Vera, "Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia" in Comparative Immunology, Microbiology and Infectious Diseases, 101 (2023):102056,
https://doi.org/10.1016/j.cimid.2023.102056 . .

TY  - CONF
AU  - Radalj, Andrea
AU  - Nišavić, Jakov
AU  - Krnjaić, Dejan
AU  - Milić, Nenad
AU  - Prošić, Isidora
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2427
AB  - Lančana reakcija polimeraze (PCR) u današnje vreme predstavlja nezaobilaznu proceduru u većini mikrobioloških laboratorija, a našla je primenu kako u bakteriološkoj, tako i u virusološkoj dijagnostici.
Prilikom ispitivanja osetljivosti bakterija na antibiotike, PCR dopunjuje
klasične metode i pruža mogućnost istovremenog ispitivanja prisustva
više genetskih determinanti rezistencije. PCR olakšava virusološku dijagnostiku kroz pravovremeno i pouzdano dobijanje rezultata ispitivanja, što često nije moguće primenom standardnih metoda. Fluorohinoloni su antibiotici širokog spektra delovanja sa baktericidnim delovanjem i često se upotrebljavaju u veterinarskoj medicini. Jedan od
mehanizama sticanja rezistencije bakterija na fluorohinolone je putem
gena u okviru plazmida koji kodiraju sintezu qnr (quinolone resistance) proteina. Tri najvažnije grupe qnr gena (qnrA, qnrB i qnrS) se
mogu istovremeno detektovati primenom multiplex PCR. Divlje svinje
i šakali su rasprostranjene vrste divljači i rezervoari značajnih virusa
među kojima su i različiti cirkovirusi i parvovirusi. Metoda PCR u cilju
detekcije svinjskog cirkovirusa 2, svinjskih parvovirusa (PPV1-PPV4),
cirkovirusa i parvovirusa pasa će se vršiti ispitivanjem ekstrakata DNK
različitih tkiva divljači, dok će se metoda multiplex PCR u cilju identifikacije qnr gena vršiti korišćenjem ekstrahovane DNK bakterija iz familije Enterobacteriaceae. PCR će se vršiti po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi vizuelizacije i analize dobijenih PCR produkata.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - PCR dijagnostika nekih vrsta virusa kod divljih životinja. Primena PCR u otkrivanju gena rezistencije bakterija na fluorohinolone
T1  - PCR diagnostics of some viruses of wildlife. The application of PCR in the detection of quinolone resistance genes
SP  - 169
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2427
ER  - 

@conference{
author = "Radalj, Andrea and Nišavić, Jakov and Krnjaić, Dejan and Milić, Nenad and Prošić, Isidora",
year = "2022",
abstract = "Lančana reakcija polimeraze (PCR) u današnje vreme predstavlja nezaobilaznu proceduru u većini mikrobioloških laboratorija, a našla je primenu kako u bakteriološkoj, tako i u virusološkoj dijagnostici.
Prilikom ispitivanja osetljivosti bakterija na antibiotike, PCR dopunjuje
klasične metode i pruža mogućnost istovremenog ispitivanja prisustva
više genetskih determinanti rezistencije. PCR olakšava virusološku dijagnostiku kroz pravovremeno i pouzdano dobijanje rezultata ispitivanja, što često nije moguće primenom standardnih metoda. Fluorohinoloni su antibiotici širokog spektra delovanja sa baktericidnim delovanjem i često se upotrebljavaju u veterinarskoj medicini. Jedan od
mehanizama sticanja rezistencije bakterija na fluorohinolone je putem
gena u okviru plazmida koji kodiraju sintezu qnr (quinolone resistance) proteina. Tri najvažnije grupe qnr gena (qnrA, qnrB i qnrS) se
mogu istovremeno detektovati primenom multiplex PCR. Divlje svinje
i šakali su rasprostranjene vrste divljači i rezervoari značajnih virusa
među kojima su i različiti cirkovirusi i parvovirusi. Metoda PCR u cilju
detekcije svinjskog cirkovirusa 2, svinjskih parvovirusa (PPV1-PPV4),
cirkovirusa i parvovirusa pasa će se vršiti ispitivanjem ekstrakata DNK
različitih tkiva divljači, dok će se metoda multiplex PCR u cilju identifikacije qnr gena vršiti korišćenjem ekstrahovane DNK bakterija iz familije Enterobacteriaceae. PCR će se vršiti po unapred uspostavljenim
protokolima uz izvođenje horizontalne gel elektroforeze radi vizuelizacije i analize dobijenih PCR produkata.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "PCR dijagnostika nekih vrsta virusa kod divljih životinja. Primena PCR u otkrivanju gena rezistencije bakterija na fluorohinolone, PCR diagnostics of some viruses of wildlife. The application of PCR in the detection of quinolone resistance genes",
pages = "169-180",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2427"
}

Radalj, A., Nišavić, J., Krnjaić, D., Milić, N.,& Prošić, I.. (2022). PCR dijagnostika nekih vrsta virusa kod divljih životinja. Primena PCR u otkrivanju gena rezistencije bakterija na fluorohinolone. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 169-180.
https://hdl.handle.net/21.15107/rcub_veterinar_2427

Radalj A, Nišavić J, Krnjaić D, Milić N, Prošić I. PCR dijagnostika nekih vrsta virusa kod divljih životinja. Primena PCR u otkrivanju gena rezistencije bakterija na fluorohinolone. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:169-180.
https://hdl.handle.net/21.15107/rcub_veterinar_2427 .

Radalj, Andrea, Nišavić, Jakov, Krnjaić, Dejan, Milić, Nenad, Prošić, Isidora, "PCR dijagnostika nekih vrsta virusa kod divljih životinja. Primena PCR u otkrivanju gena rezistencije bakterija na fluorohinolone" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):169-180,
https://hdl.handle.net/21.15107/rcub_veterinar_2427 .

TY  - CONF
AU  - Radojičić, Marina
AU  - Prošić, Isidora
AU  - Ezveđ, Jožef
AU  - Krnjaić, Dejan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3144
AB  - Prisustvo infektivnih bolesti u zoološkim vrtovima ugrožava dobrobit životinja i 
ima dalekosežne posledice po životni vek, reprodukciju i ponašanje životinja. Najčešće 
izolovana vrsta koja izaziva mikozu ptica je Aspergillus fumigatus koji je izolovan u 
95% slučajeva aspergiloze ptica. Nakon prelaska u zatvoren prostor, kod nekoliko pin gvina u zoološkom vrtu pojavili su se respiratorni simptomi. Nakon uzorkovanja bri seva površina i vazduha za mikrobiološki pregled, izvršena je kompletna dezinfekcija 
prostora i opreme, a nakon završne dezinfekcije sprovedena je njena kontrola. Identi fikacija mikroorganizama izvršena je na osnovu morfologije izraslih kolonija, kao i na 
osnovu mikroskopskog pregleda preparata, a broj kolonija određen je prema standar dnim procedurama. Dezinfekcija je izvršena organskim proizvodom koji sadrži organ sku melasu šećerne trske i kulture probiotskih mikroorganizama. Pored dezinfekcije, 
izvršena je i kompletna zamena ventilacionih cevi i filtera za vazduh. Procesi uzorko vanja i identifikacija izraslih kolonija ponovlјeni su nakon dva meseca, odnosno 30 
dana nakon izvršene dezinfekcije, u sklopu kontrole dezinfekcije. Rezultati dobijeni 
pre izvršene dezinfekcije pokazali su povećan broj kolonija A. fumigatus u većini uzo raka. Takođe, primećeno je da je najveći broj kolonija detektovan u podlogama u ko jima su zasejani brisevi uzeti sa ventilacionih otvora kao i iz uzoraka vazduha koji su 
prikuplјeni u blizini ventilacionih cevi. Iz tog razloga, preporučena je detalјna zamena 
kompletnog sistema za ventilaciju. Nakon 30 dana od izvršene dezinfekcije urađena 
je kontorola dezinfekcije gde je u svim uzorcima detektovan očekivan broj kolonija A. 
fumigatus, koji ne predstavlјa pretnju po okolni živi svet, čime je potvrđena efikasnost 
izvršene dezinfekcije i primenjenog dezificijensa. Potrebno je naglasiti da, iako su svi 
uslovi u smeštaju za držanje pingvina adekvatni, ipak može doći do infekcije pingvina 
sa A. fumigatus jer su ptice, a posebno one u zatočeništvu, predisponirane ka infekciji 
ovim uzročnikom o čemu svedoči više slučajeva izbijanja zaraze sa A. fumigatus kod 
pingvina u zoološkim vrtovima.
AB  - The spread of infectious diseases in zoo gardens endangers animals’ welfare and 
has significant consequences on their lifespan, reproduction and behavior. Bird mycosis is frequently associated with Aspergillus fumigatus, which is the etiologic agent 
in 95% of all aspergilosis cases in birds. Respiratory signs had been observed in penguins in the zoo, after the transfer to their enclosure. Air and surface swabs were taken for microbiological examination, after which complete desinfection of the enclosure and equipment was performed. Following desinfection, control swabs were taken in order to check the desinfection efficasy. Identification of the microorganisms was based on colony morpholgy and examination of microscopic slide preparations, and colony number was determined by using standard procedures. The desinfectant is an organic compound which contains organic sugar cane molasses and probiotic microorganism cultures. Beside disinfection, complete replacement of ventilation pipes and air filters was performed. Each procedure was repeated after two months, ie 30 days after disinfection, as part of disinfection control. The results showed an increased number of A. fumigatus colonies in most samples. Moreover, it was noticed that the largest number of colonies was detected in swab samples from ventilation and air samples collected near ventilation pipes. For this reason, replacement of the complete ventilation system was recommended. Thirty days after disinfection, another control was performed, where an expected number of A. fumigatus colonies was detected in all samples, which does not pose a threat for animals or visitors, thus the effectiveness of the disinfection and the disinfectant was confirmed. It should be emphasized that, 
although all the conditions in the penguin enclosure are adequate, an infection with A. 
fumigatus can occur in penguins because birds, especially those in captivity, are predisposed to infection with this pathogen, as evidenced by several outbreaks of A. fumigatus in penguins in zoo gardens.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022
T1  - Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja
SP  - 119
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3144
ER  - 

@conference{
author = "Radojičić, Marina and Prošić, Isidora and Ezveđ, Jožef and Krnjaić, Dejan",
year = "2022",
abstract = "Prisustvo infektivnih bolesti u zoološkim vrtovima ugrožava dobrobit životinja i 
ima dalekosežne posledice po životni vek, reprodukciju i ponašanje životinja. Najčešće 
izolovana vrsta koja izaziva mikozu ptica je Aspergillus fumigatus koji je izolovan u 
95% slučajeva aspergiloze ptica. Nakon prelaska u zatvoren prostor, kod nekoliko pin gvina u zoološkom vrtu pojavili su se respiratorni simptomi. Nakon uzorkovanja bri seva površina i vazduha za mikrobiološki pregled, izvršena je kompletna dezinfekcija 
prostora i opreme, a nakon završne dezinfekcije sprovedena je njena kontrola. Identi fikacija mikroorganizama izvršena je na osnovu morfologije izraslih kolonija, kao i na 
osnovu mikroskopskog pregleda preparata, a broj kolonija određen je prema standar dnim procedurama. Dezinfekcija je izvršena organskim proizvodom koji sadrži organ sku melasu šećerne trske i kulture probiotskih mikroorganizama. Pored dezinfekcije, 
izvršena je i kompletna zamena ventilacionih cevi i filtera za vazduh. Procesi uzorko vanja i identifikacija izraslih kolonija ponovlјeni su nakon dva meseca, odnosno 30 
dana nakon izvršene dezinfekcije, u sklopu kontrole dezinfekcije. Rezultati dobijeni 
pre izvršene dezinfekcije pokazali su povećan broj kolonija A. fumigatus u većini uzo raka. Takođe, primećeno je da je najveći broj kolonija detektovan u podlogama u ko jima su zasejani brisevi uzeti sa ventilacionih otvora kao i iz uzoraka vazduha koji su 
prikuplјeni u blizini ventilacionih cevi. Iz tog razloga, preporučena je detalјna zamena 
kompletnog sistema za ventilaciju. Nakon 30 dana od izvršene dezinfekcije urađena 
je kontorola dezinfekcije gde je u svim uzorcima detektovan očekivan broj kolonija A. 
fumigatus, koji ne predstavlјa pretnju po okolni živi svet, čime je potvrđena efikasnost 
izvršene dezinfekcije i primenjenog dezificijensa. Potrebno je naglasiti da, iako su svi 
uslovi u smeštaju za držanje pingvina adekvatni, ipak može doći do infekcije pingvina 
sa A. fumigatus jer su ptice, a posebno one u zatočeništvu, predisponirane ka infekciji 
ovim uzročnikom o čemu svedoči više slučajeva izbijanja zaraze sa A. fumigatus kod 
pingvina u zoološkim vrtovima., The spread of infectious diseases in zoo gardens endangers animals’ welfare and 
has significant consequences on their lifespan, reproduction and behavior. Bird mycosis is frequently associated with Aspergillus fumigatus, which is the etiologic agent 
in 95% of all aspergilosis cases in birds. Respiratory signs had been observed in penguins in the zoo, after the transfer to their enclosure. Air and surface swabs were taken for microbiological examination, after which complete desinfection of the enclosure and equipment was performed. Following desinfection, control swabs were taken in order to check the desinfection efficasy. Identification of the microorganisms was based on colony morpholgy and examination of microscopic slide preparations, and colony number was determined by using standard procedures. The desinfectant is an organic compound which contains organic sugar cane molasses and probiotic microorganism cultures. Beside disinfection, complete replacement of ventilation pipes and air filters was performed. Each procedure was repeated after two months, ie 30 days after disinfection, as part of disinfection control. The results showed an increased number of A. fumigatus colonies in most samples. Moreover, it was noticed that the largest number of colonies was detected in swab samples from ventilation and air samples collected near ventilation pipes. For this reason, replacement of the complete ventilation system was recommended. Thirty days after disinfection, another control was performed, where an expected number of A. fumigatus colonies was detected in all samples, which does not pose a threat for animals or visitors, thus the effectiveness of the disinfection and the disinfectant was confirmed. It should be emphasized that, 
although all the conditions in the penguin enclosure are adequate, an infection with A. 
fumigatus can occur in penguins because birds, especially those in captivity, are predisposed to infection with this pathogen, as evidenced by several outbreaks of A. fumigatus in penguins in zoo gardens.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022",
title = "Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja",
pages = "119-125",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3144"
}

Radojičić, M., Prošić, I., Ezveđ, J.,& Krnjaić, D.. (2022). Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja. in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022
Beograd : Srpsko veterinarsko društvo., 119-125.
https://hdl.handle.net/21.15107/rcub_veterinar_3144

Radojičić M, Prošić I, Ezveđ J, Krnjaić D. Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja. in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022. 2022;:119-125.
https://hdl.handle.net/21.15107/rcub_veterinar_3144 .

Radojičić, Marina, Prošić, Isidora, Ezveđ, Jožef, Krnjaić, Dejan, "Značaj sprovođenja dezinfekcije u zoo vrtovima – aspergiloza pingvina (Spheniscus humboldti) – prikaz slučaja" in 33. Savetovanje Dezinfekcija, dezinsekcija i deratizacija - Jedan svet jedno zdravlje, Bajina Bašta, 26 - 29. maj 2022 (2022):119-125,
https://hdl.handle.net/21.15107/rcub_veterinar_3144 .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Krnjaić, Dejan
AU  - Milićević, Dragan
AU  - Knežević, Aleksandra
AU  - Radojičić, Marina
AU  - Obrenović, Sonja
AU  - Ćosić, Milivoje
AU  - Tešović, Bojana
AU  - Benković, Damir
AU  - Živulj Aleksandar
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2044
AB  - Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.
AB  - Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia
T1  - Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji
VL  - 71
IS  - 1
SP  - 32
EP  - 46
DO  - 10.2478/acve-2021-0003
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Krnjaić, Dejan and Milićević, Dragan and Knežević, Aleksandra and Radojičić, Marina and Obrenović, Sonja and Ćosić, Milivoje and Tešović, Bojana and Benković, Damir and Živulj Aleksandar",
year = "2021",
abstract = "Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars., Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia, Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji",
volume = "71",
number = "1",
pages = "32-46",
doi = "10.2478/acve-2021-0003"
}

Nišavić, J., Milić, N., Radalj, A., Krnjaić, D., Milićević, D., Knežević, A., Radojičić, M., Obrenović, S., Ćosić, M., Tešović, B., Benković, D.,& Živulj Aleksandar. (2021). Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 32-46.
https://doi.org/10.2478/acve-2021-0003

Nišavić J, Milić N, Radalj A, Krnjaić D, Milićević D, Knežević A, Radojičić M, Obrenović S, Ćosić M, Tešović B, Benković D, Živulj Aleksandar. Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd. 2021;71(1):32-46.
doi:10.2478/acve-2021-0003 .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Krnjaić, Dejan, Milićević, Dragan, Knežević, Aleksandra, Radojičić, Marina, Obrenović, Sonja, Ćosić, Milivoje, Tešović, Bojana, Benković, Damir, Živulj Aleksandar, "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia" in Acta Veterinaria-Beograd, 71, no. 1 (2021):32-46,
https://doi.org/10.2478/acve-2021-0003 . .

TY  - CONF
AU  - Radojičić, Marina
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Nišavić, Jakov
AU  - Radalj, Andrea
AU  - Prošić, Isidora
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2263
AB  - Today, pets are the source of numerous infectious diseases that can transmissible
to humans resulting from their increasingly frequent contact. The most notable
viruses with zoonotic potential include rabies and influenza viruses as well as
rotaviruses and noroviruses. However, the importance of individual viruses varies
depending on the climate and infectious disease control systems in certain
countries. Dogs, cats, and other increasingly popular pet species can transmit
bacterial zoonotic agents to humans in various ways. Aside from well-known
pathogens such as the bacteria causing leptospirosis, salmonellosis,
campylobacteriosis, or brucellosis, the bacteria Pasteurella multocida and
Bartonella henselae transmitted by bites or scratches are also significant in
human pathology. There has been a notable increase in the prevalence of
methicillin-resistant strains of Staphylococcus aureus (MRSA) in isolates
originating from pets and the transmission of these strains between humans and
animals requires special attention. Furthermore, fungi causing diseases such as
sporotrichosis or dermatophytosis are linked to long-term and persistent
infections in humans. The epidemiological situation caused by SARS-CoV-2, and
the assumption of an interspecies jump of this virus from animals to humans,
including its documented presence in domestic cats, dogs, tigers, and martens,
have raised the question of the possibility of virus transmission from pets to
humans. However, the current pandemic is caused solely by SARS-CoV-2
transmission in the human population, and these animals are not a source of infection for humans. A significant number of zoonoses originating from pets
pose a threat to public health, thus requiring the "One Health" approach through
close cooperation between human and veterinary medicine to develop and
implement effective health measures for both humans and animals. Veterinarians
must inform pet owners about infectious disease prevention measures and risks
posed by keeping certain species of animals as a part of responsible ownership.
AB  - Кућни љубимци у данашње време представљају извор великог броја
инфективних обољења преносивих на људе као последица њиховог све
учесталијег контакта. У најважније вирусе са зоонотским потенцијалом
спадају вируси беснила и инфлуенце као и ротавируси и норовируси,
међутим, значај појединих вируса варира зависно од поднебља као и система
сузбијања заразних болести у појединим државама. Пси и мачке, као и све
популарније остале врсте љубимаца, на различите начине могу пренети
узрочнике бактеријских зооноза на човека. Тако на пример, бактерије које се
преносе уједом или путем огреботина као што су Pasteurella multocida или
Bartonella henselae, поред познатих узрочника обољења попут лептоспирозе,
салмонелозе, кампилобактериозе или бруцелозе, немају занемарљив значај у
патологији људи. Последњих година је доказан пораст преваленције
метицилин резистентних сојева Staphylococcus aureus (MRSA) у изолатима
пореклом од кућних љубимаца, а великa пажња се придаје управо трансмисији
ових сојева између људи и животиња. Поред бактерија и вируса, гљивице, као
што су узрочници споротрихозе или дерматофитозе, често представљају узрок
дуготрајних и упорних инфекција људи. Епидемиолошка ситуација у свету
изазвана SARS-CoV-2 и претпоставка о интерспецијском скоку овог вируса са
животиња на човека, као и његово присуство детектовно код домаћих мачака,
паса, тигрова и куна, довеле су у питање могућност преношења вируса са
кућних љубимаца на људе. Неопходно је напоменути да је тренутна пандемија изазвана искључиво трансмисијом SARS-CoV-2 у хуманој популацији и да
наведене животиње нису извор инфекције за човека. Значајан број зооноза
пореклом од кућних љубимаца представља претњу по јавно здравље при чему
је неопходна примена концепта јединственог здравља која подразумева
блиску сарадњу хумане и ветеринарске медицине са циљем развоја и примене
ефикасних мера здравствене заштите људи и животиња. У склопу одговорног
власништва, власници кућних љубимаца од стране ветеринара морају бити
обавештени о мерама превенције инфективних болести и појединим
ризицима које носи држање одређених врста животиња.
C3  - 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske
T1  - Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca
T1  - Bacterial, fungal, and viral zoonoses of pets
SP  - 49
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2263
ER  - 

@conference{
author = "Radojičić, Marina and Milić, Nenad and Krnjaić, Dejan and Nišavić, Jakov and Radalj, Andrea and Prošić, Isidora",
year = "2021",
abstract = "Today, pets are the source of numerous infectious diseases that can transmissible
to humans resulting from their increasingly frequent contact. The most notable
viruses with zoonotic potential include rabies and influenza viruses as well as
rotaviruses and noroviruses. However, the importance of individual viruses varies
depending on the climate and infectious disease control systems in certain
countries. Dogs, cats, and other increasingly popular pet species can transmit
bacterial zoonotic agents to humans in various ways. Aside from well-known
pathogens such as the bacteria causing leptospirosis, salmonellosis,
campylobacteriosis, or brucellosis, the bacteria Pasteurella multocida and
Bartonella henselae transmitted by bites or scratches are also significant in
human pathology. There has been a notable increase in the prevalence of
methicillin-resistant strains of Staphylococcus aureus (MRSA) in isolates
originating from pets and the transmission of these strains between humans and
animals requires special attention. Furthermore, fungi causing diseases such as
sporotrichosis or dermatophytosis are linked to long-term and persistent
infections in humans. The epidemiological situation caused by SARS-CoV-2, and
the assumption of an interspecies jump of this virus from animals to humans,
including its documented presence in domestic cats, dogs, tigers, and martens,
have raised the question of the possibility of virus transmission from pets to
humans. However, the current pandemic is caused solely by SARS-CoV-2
transmission in the human population, and these animals are not a source of infection for humans. A significant number of zoonoses originating from pets
pose a threat to public health, thus requiring the "One Health" approach through
close cooperation between human and veterinary medicine to develop and
implement effective health measures for both humans and animals. Veterinarians
must inform pet owners about infectious disease prevention measures and risks
posed by keeping certain species of animals as a part of responsible ownership., Кућни љубимци у данашње време представљају извор великог броја
инфективних обољења преносивих на људе као последица њиховог све
учесталијег контакта. У најважније вирусе са зоонотским потенцијалом
спадају вируси беснила и инфлуенце као и ротавируси и норовируси,
међутим, значај појединих вируса варира зависно од поднебља као и система
сузбијања заразних болести у појединим државама. Пси и мачке, као и све
популарније остале врсте љубимаца, на различите начине могу пренети
узрочнике бактеријских зооноза на човека. Тако на пример, бактерије које се
преносе уједом или путем огреботина као што су Pasteurella multocida или
Bartonella henselae, поред познатих узрочника обољења попут лептоспирозе,
салмонелозе, кампилобактериозе или бруцелозе, немају занемарљив значај у
патологији људи. Последњих година је доказан пораст преваленције
метицилин резистентних сојева Staphylococcus aureus (MRSA) у изолатима
пореклом од кућних љубимаца, а великa пажња се придаје управо трансмисији
ових сојева између људи и животиња. Поред бактерија и вируса, гљивице, као
што су узрочници споротрихозе или дерматофитозе, често представљају узрок
дуготрајних и упорних инфекција људи. Епидемиолошка ситуација у свету
изазвана SARS-CoV-2 и претпоставка о интерспецијском скоку овог вируса са
животиња на човека, као и његово присуство детектовно код домаћих мачака,
паса, тигрова и куна, довеле су у питање могућност преношења вируса са
кућних љубимаца на људе. Неопходно је напоменути да је тренутна пандемија изазвана искључиво трансмисијом SARS-CoV-2 у хуманој популацији и да
наведене животиње нису извор инфекције за човека. Значајан број зооноза
пореклом од кућних љубимаца представља претњу по јавно здравље при чему
је неопходна примена концепта јединственог здравља која подразумева
блиску сарадњу хумане и ветеринарске медицине са циљем развоја и примене
ефикасних мера здравствене заштите људи и животиња. У склопу одговорног
власништва, власници кућних љубимаца од стране ветеринара морају бити
обавештени о мерама превенције инфективних болести и појединим
ризицима које носи држање одређених врста животиња.",
journal = "26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske",
title = "Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca, Bacterial, fungal, and viral zoonoses of pets",
pages = "49-52",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2263"
}

Radojičić, M., Milić, N., Krnjaić, D., Nišavić, J., Radalj, A.,& Prošić, I.. (2021). Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca. in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske, 49-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2263

Radojičić M, Milić N, Krnjaić D, Nišavić J, Radalj A, Prošić I. Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca. in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske. 2021;:49-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2263 .

Radojičić, Marina, Milić, Nenad, Krnjaić, Dejan, Nišavić, Jakov, Radalj, Andrea, Prošić, Isidora, "Bakterijske, gljivične i virusne zoonoze kućnih ljubimaca" in 26. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske (2021):49-52,
https://hdl.handle.net/21.15107/rcub_veterinar_2263 .

TY  - JOUR
AU  - Bošković, Marija
AU  - Đorđević, Jasna
AU  - Glišić, Milica
AU  - Ćirić, Jelena
AU  - Janjić, Jelena
AU  - Zdravković, Nemanja
AU  - Krnjaić, Dejan
AU  - Baltić, Milan Ž.
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1800
AB  - The survival of Salmonella Enteritidis, S. Typhimurium, S. Infantis, and S. Montevideo in experimentally inoculated (10(6) CFU/g) minced pork meat containing different concentrations (0%, 0.3%, 0.6%, or 0.9%) of oregano (Origanum vulgare) essential oil (OEO) packaged under vacuum or MAP (30%O-2/50%CO2/20%N-2) were evaluated within 15 days of refrigeration (3 +/- 1 degrees C) storage. Carvacrol was the predominant constituent in OEO (77.16%). The minimum inhibitory concentration of OEO was similar for all Salmonella serovars (160-320 mu g/ml). Salmonella counts in minced pork were reduced by 2.09 and 3.08 log CFU/g in the samples treated with 0.3% and 0.6% OEO, respectively. Addition of 0.9% OEO reduced the Salmonella count to below the limit of detection. Experiments also included investigation of OEO effect on Enterobacteriaceae, LAB and TVC. For both packaging methods the use of OEO increased the microbial shelf life of pork. Sensory analyses showed that the samples with 0.3% OEO added were the most acceptable. Practical applications Due to its nutritional characteristics, minced meat is suitable for the growth of most microorganisms including spoilage bacteria and foodborne pathogens such as Salmonella spp. The microbial safety of minced meat is very important. Taking into account that these bacteria are one of the most important from the aspect of meat safety, their control, especially in minimally processed meat products, is crucial. OEO not only exhibited strong anti-Salmonella effects, but, in contrast to some other essential oils, is categorized as GRAS (generally recognized as safe) and can be considered as a natural preservative used in organic production as an alternative to synthetic additives. Our findings could also guide the selection of an effective concentration of OEO that is organoleptically acceptable.
PB  - Wiley, Hoboken
T2  - Journal of Food Processing and Preservation
T1  - The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere
VL  - 44
IS  - 1
SP  - e14311
DO  - 10.1111/jfpp.14311
ER  - 

@article{
author = "Bošković, Marija and Đorđević, Jasna and Glišić, Milica and Ćirić, Jelena and Janjić, Jelena and Zdravković, Nemanja and Krnjaić, Dejan and Baltić, Milan Ž.",
year = "2020",
abstract = "The survival of Salmonella Enteritidis, S. Typhimurium, S. Infantis, and S. Montevideo in experimentally inoculated (10(6) CFU/g) minced pork meat containing different concentrations (0%, 0.3%, 0.6%, or 0.9%) of oregano (Origanum vulgare) essential oil (OEO) packaged under vacuum or MAP (30%O-2/50%CO2/20%N-2) were evaluated within 15 days of refrigeration (3 +/- 1 degrees C) storage. Carvacrol was the predominant constituent in OEO (77.16%). The minimum inhibitory concentration of OEO was similar for all Salmonella serovars (160-320 mu g/ml). Salmonella counts in minced pork were reduced by 2.09 and 3.08 log CFU/g in the samples treated with 0.3% and 0.6% OEO, respectively. Addition of 0.9% OEO reduced the Salmonella count to below the limit of detection. Experiments also included investigation of OEO effect on Enterobacteriaceae, LAB and TVC. For both packaging methods the use of OEO increased the microbial shelf life of pork. Sensory analyses showed that the samples with 0.3% OEO added were the most acceptable. Practical applications Due to its nutritional characteristics, minced meat is suitable for the growth of most microorganisms including spoilage bacteria and foodborne pathogens such as Salmonella spp. The microbial safety of minced meat is very important. Taking into account that these bacteria are one of the most important from the aspect of meat safety, their control, especially in minimally processed meat products, is crucial. OEO not only exhibited strong anti-Salmonella effects, but, in contrast to some other essential oils, is categorized as GRAS (generally recognized as safe) and can be considered as a natural preservative used in organic production as an alternative to synthetic additives. Our findings could also guide the selection of an effective concentration of OEO that is organoleptically acceptable.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Processing and Preservation",
title = "The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere",
volume = "44",
number = "1",
pages = "e14311",
doi = "10.1111/jfpp.14311"
}

Bošković, M., Đorđević, J., Glišić, M., Ćirić, J., Janjić, J., Zdravković, N., Krnjaić, D.,& Baltić, M. Ž.. (2020). The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere. in Journal of Food Processing and Preservation
Wiley, Hoboken., 44(1), e14311.
https://doi.org/10.1111/jfpp.14311

Bošković M, Đorđević J, Glišić M, Ćirić J, Janjić J, Zdravković N, Krnjaić D, Baltić MŽ. The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere. in Journal of Food Processing and Preservation. 2020;44(1):e14311.
doi:10.1111/jfpp.14311 .

Bošković, Marija, Đorđević, Jasna, Glišić, Milica, Ćirić, Jelena, Janjić, Jelena, Zdravković, Nemanja, Krnjaić, Dejan, Baltić, Milan Ž., "The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere" in Journal of Food Processing and Preservation, 44, no. 1 (2020):e14311,
https://doi.org/10.1111/jfpp.14311 . .

TY  - JOUR
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Ivetić, Vojin
AU  - Krnjaić, Dejan
AU  - Radanović, Oliver
AU  - Žutić, Jadranka
AU  - Spalević, Ljiljana
AU  - Jovanović, Milijan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1748
AB  - The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus
VL  - 69
IS  - 2
SP  - 201
EP  - 217
DO  - 10.2478/acve-2019-0016
ER  - 

@article{
author = "Jezdimirović, Nemanja and Kureljušić, Branislav and Ivetić, Vojin and Krnjaić, Dejan and Radanović, Oliver and Žutić, Jadranka and Spalević, Ljiljana and Jovanović, Milijan",
year = "2019",
abstract = "The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056x10(7) spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus",
volume = "69",
number = "2",
pages = "201-217",
doi = "10.2478/acve-2019-0016"
}

Jezdimirović, N., Kureljušić, B., Ivetić, V., Krnjaić, D., Radanović, O., Žutić, J., Spalević, L.,& Jovanović, M.. (2019). Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 201-217.
https://doi.org/10.2478/acve-2019-0016

Jezdimirović N, Kureljušić B, Ivetić V, Krnjaić D, Radanović O, Žutić J, Spalević L, Jovanović M. Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus. in Acta Veterinaria-Beograd. 2019;69(2):201-217.
doi:10.2478/acve-2019-0016 .

Jezdimirović, Nemanja, Kureljušić, Branislav, Ivetić, Vojin, Krnjaić, Dejan, Radanović, Oliver, Žutić, Jadranka, Spalević, Ljiljana, Jovanović, Milijan, "Comparative pathomorphological, mycological and molecular examination of turkey poults with different immunological status experimentally infected with aspergillus fumigatus" in Acta Veterinaria-Beograd, 69, no. 2 (2019):201-217,
https://doi.org/10.2478/acve-2019-0016 . .

TY  - JOUR
AU  - Pavličević, Aleksandar
AU  - Pavlović, Ivan
AU  - Ratajac, Radomir
AU  - Popović, Danica
AU  - Davidović, Branislav
AU  - Krnjaić, Dejan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1951
AB  - Technological solutions and environmental conditions have a significant impact on infestation intensity and the problems around D. gallinae control. Changes in keeping laying hens in EU, in terms of D. gallinae influence, have not led to the welfare of the layers. On the contrary, they have contributed to the spreading of disease, have worsened conditions for control and accentuated harmful consequences. Apart from the poultry, these changes have also had a negative impact on the welfare of humans, through a toxicological and zootonic risk, and economic damages. Conventional cages so far provide the most appropriate environment for D. gallinae control. Opportunities for improving, even solving the problem of D. gallinae control in egg production do exist, however they require a changing the entire approach hitherto.
AB  - Tehnološka rešenja i ambijentalne prilike bitno utiču na intezitet infestacije i problematičnost kontrole D. gallinae. Promene u načinu držanja kokošaka nosilja u EU, sa aspekta uticaja D. gallinae, nisu dovele do dobrobiti nosilja. Naprotiv, doprinele su širenju bolesti, pogoršale uslove kontrole i naglasile štetne posledice. Osim na živinu, promene su nepovoljno uticale na dobrobit čoveka kroz toksikološki i zoonotski rizik, i ekonomske štete. Konvencionalni kavezi obezbeđuju do sad najprikladniji ambijent za kontrolu D. gallinae. Mogućnosti za unapređenje, pa i rešenje kontrole D. gallinae u proizvodnji jaja postoje, ali ona zahteva promenu celokupnog dosadašnjeg pristupa.
PB  - Institut za stočarstvo
T2  - Biotechnology in Animal Husbandry
T1  - Poultry welfare in terms of poultry red mite (Dermanyssus gallinae) impact and control
T1  - Prilog razmatranju dobrobiti živine sa aspekta uticaja i kontrole crvene kokošije grinje (Dermanyssus gallinae)
VL  - 35
IS  - 1
SP  - 1
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1951
ER  - 

@article{
author = "Pavličević, Aleksandar and Pavlović, Ivan and Ratajac, Radomir and Popović, Danica and Davidović, Branislav and Krnjaić, Dejan",
year = "2019",
abstract = "Technological solutions and environmental conditions have a significant impact on infestation intensity and the problems around D. gallinae control. Changes in keeping laying hens in EU, in terms of D. gallinae influence, have not led to the welfare of the layers. On the contrary, they have contributed to the spreading of disease, have worsened conditions for control and accentuated harmful consequences. Apart from the poultry, these changes have also had a negative impact on the welfare of humans, through a toxicological and zootonic risk, and economic damages. Conventional cages so far provide the most appropriate environment for D. gallinae control. Opportunities for improving, even solving the problem of D. gallinae control in egg production do exist, however they require a changing the entire approach hitherto., Tehnološka rešenja i ambijentalne prilike bitno utiču na intezitet infestacije i problematičnost kontrole D. gallinae. Promene u načinu držanja kokošaka nosilja u EU, sa aspekta uticaja D. gallinae, nisu dovele do dobrobiti nosilja. Naprotiv, doprinele su širenju bolesti, pogoršale uslove kontrole i naglasile štetne posledice. Osim na živinu, promene su nepovoljno uticale na dobrobit čoveka kroz toksikološki i zoonotski rizik, i ekonomske štete. Konvencionalni kavezi obezbeđuju do sad najprikladniji ambijent za kontrolu D. gallinae. Mogućnosti za unapređenje, pa i rešenje kontrole D. gallinae u proizvodnji jaja postoje, ali ona zahteva promenu celokupnog dosadašnjeg pristupa.",
publisher = "Institut za stočarstvo",
journal = "Biotechnology in Animal Husbandry",
title = "Poultry welfare in terms of poultry red mite (Dermanyssus gallinae) impact and control, Prilog razmatranju dobrobiti živine sa aspekta uticaja i kontrole crvene kokošije grinje (Dermanyssus gallinae)",
volume = "35",
number = "1",
pages = "1-11",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1951"
}

Pavličević, A., Pavlović, I., Ratajac, R., Popović, D., Davidović, B.,& Krnjaić, D.. (2019). Poultry welfare in terms of poultry red mite (Dermanyssus gallinae) impact and control. in Biotechnology in Animal Husbandry
Institut za stočarstvo., 35(1), 1-11.
https://hdl.handle.net/21.15107/rcub_veterinar_1951

Pavličević A, Pavlović I, Ratajac R, Popović D, Davidović B, Krnjaić D. Poultry welfare in terms of poultry red mite (Dermanyssus gallinae) impact and control. in Biotechnology in Animal Husbandry. 2019;35(1):1-11.
https://hdl.handle.net/21.15107/rcub_veterinar_1951 .

Pavličević, Aleksandar, Pavlović, Ivan, Ratajac, Radomir, Popović, Danica, Davidović, Branislav, Krnjaić, Dejan, "Poultry welfare in terms of poultry red mite (Dermanyssus gallinae) impact and control" in Biotechnology in Animal Husbandry, 35, no. 1 (2019):1-11,
https://hdl.handle.net/21.15107/rcub_veterinar_1951 .

TY  - JOUR
AU  - Radalj, Andrea
AU  - Nišavić, Jakov
AU  - Krnjaić, Dejan
AU  - Valčić, Miroslav
AU  - Jovanović, Tanja
AU  - Veljović, Ljubiša
AU  - Milić, Nenad
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1626
AB  - The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria - Brno
T1  - Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia
VL  - 87
IS  - 1
SP  - 27
DO  - 10.2754/avb201887010027
ER  - 

@article{
author = "Radalj, Andrea and Nišavić, Jakov and Krnjaić, Dejan and Valčić, Miroslav and Jovanović, Tanja and Veljović, Ljubiša and Milić, Nenad",
year = "2018",
abstract = "The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria - Brno",
title = "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia",
volume = "87",
number = "1",
pages = "27",
doi = "10.2754/avb201887010027"
}

Radalj, A., Nišavić, J., Krnjaić, D., Valčić, M., Jovanović, T., Veljović, L.,& Milić, N.. (2018). Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(1), 27.
https://doi.org/10.2754/avb201887010027

Radalj A, Nišavić J, Krnjaić D, Valčić M, Jovanović T, Veljović L, Milić N. Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno. 2018;87(1):27.
doi:10.2754/avb201887010027 .

Radalj, Andrea, Nišavić, Jakov, Krnjaić, Dejan, Valčić, Miroslav, Jovanović, Tanja, Veljović, Ljubiša, Milić, Nenad, "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia" in Acta Veterinaria - Brno, 87, no. 1 (2018):27,
https://doi.org/10.2754/avb201887010027 . .

TY  - CONF
AU  - Radojičić, Marina
AU  - Marković, Maja
AU  - Milić, Nenad
AU  - Kulišić, Zoran
AU  - Radalj, Andrea
AU  - Krnjaić, Dejan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2265
AB  - Mnogi zdravstveni problemi ljudi predstavljaju direktnu posledicu intezivnog kontakta sa
životinjama, pri čemu najmanje 75% novih infektivnih i pretećih infektivnih bolesti pripada
zoonozama ili vektorski prenosivim bolestima. Kućni ljubimci na različite načine ljudima mogu
preneti veliki broj infektivnih oboljenja. U najznačajnije zoonoze se svrstavaju infekcije izazvane
virusom besnila, norovirusima, rotavirusima ili virusima influence, kao i veliki broj bakterijskih
infekcija kao što su salmoneloza, kampilobakterioza, bruceloza i leptospiroza. Bakterijske
zoonoze ukjlučuju i bakterije koje se prenose ujedom ili putem ogrebotina kao što su Pasteurella
multocida ili Bartonella henselae. Sve veći značaj pridaje se transmisiji meticilin rezistentnih
sojeva Staphylococcus aureus (MRSA) između ljudi i životinja, a dokazan je i porast prevalencije
ovih bakterija u izolatima poreklom iz kućnih ljubimaca. Ne treba zanemariti ni činjenicu da psi i
mačke takođe mogu biti izvor gljivičnih infekcija kao što su sporotrihoza ili dermatofitoza. U
najznačajnije zoonotske patogene parazitske etiologije spadaju uzročnici toksoplazmoze,
đardioze, toksokarioze, onhocerkoze i ehinokokoze. Pored toga, sa globalnim otopljavanjem sve
su rasprostranjenije i vektorski prenosive zoonoze izazvane sa: Anaplasma phagocytophilum,
Rickettsia felis, Borrelia burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis
i Dirofilaria repens koje se putem krpelja, buva, peščanih mušica i komaraca prenose sa kućnih
ljubimaca na ljude. S obzirom da veliki broj pomenutih zoonoza predstavlja pretnju po javno
zdravlje, neophodno je pristupiti ovom problemu sa dve strane. Veterinari moraju upoznati
vlasnike sa metodama prevencije infektivnih bolesti kućnih ljubimaca, a koje podrazumevaju
odgovorno vlasništvo. S druge strane, lekari moraju imati u vidu mogućnost pojave zoonoza i
savetovati pacijente u cilju sprečavanja infekcije ljudi. Primenom koncepta jedinstvenog zdravlja
neophodno je uspostavljanje bliže saradnje lekara i veterinara, a jačanjem epidemioloških i
laboratorijskih ispitivanja moguće je utvrđivanje prisustva, raširenosti i rizika od pojave zoonoza
ljudi poreklom od kućnih ljubimaca, a zajedničkim radom mogu se razviti i primeniti održive i
efektivne mere zaštite zdravlja ljudi i životinja.
AB  - Many emerging health issues are linked to increasing contact between humans and animals, and
it is estimated that at least 75% of emerging and re-emerging diseases are either zoonotic or
vector-borne. Pets may transmit a variety of infectious diseases to humans. Viral diseases caused
by rabies virus, noroviruses, rotaviruses or influenza viruses, as well as numerous bacterial
infections such as salmonellosis, campylobacteriosis, brucellosis and leptospirosis present some
of the most significant zoonoses linked to household pets. Bacterial zoonoses also include bacteria
transmitted by bites or scratches like Pasteurella multocida or Bartonella henselae. More attention
is paid to the transmission of methicilin resistant strains of Staphylococcus aureus (MRSA)
between humans and animals, since the prevalence of these isolates from samples of animal origin
is on the rise. Moreover, the fact that dogs and cats can be sources of serious fungal infections such
as sporotrichosis or dermatophytosis shouldn’t be overseen. Most important zoonotic parasites
are the causative agents of toxoplasmosis, giardiasis, toxocariasis, onchocercosis and
echinococcosis. One of the apparent consequences of global warming is the increased prevalence
of vector borne zoonotic diseases caused by: Anaplasma phagocytophilum, Rickettsia felis, Borrelia
burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis and Dirofilaria repens
transmitted from animals to humans by ticks, fleas, phlebotomine sand flies and mosquitoes.
Considering that many of the mentioned zoonoses pose a serious risk to public health, it is
essential to address this problem from two sides. Veterinarians must instruct the owners on
necessary preventive measures implying responsible pet ownership. On the other hand,
physicians must maintain awareness of pet infectious diseases and counsel patients to prevent
human infection. By strengthening epidemiologic and laboratory investigations that assess the
role of environmental influences, this partnership can help develop and apply sustainable and
effective community health interventions.
PB  - Udruženje mikrobiologa Srbije, Beograd
C3  - XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
T1  - Kućni ljubimci i zoonoze
T1  - Pets and zoonoses
SP  - 153
EP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2265
ER  - 

@conference{
author = "Radojičić, Marina and Marković, Maja and Milić, Nenad and Kulišić, Zoran and Radalj, Andrea and Krnjaić, Dejan",
year = "2018",
abstract = "Mnogi zdravstveni problemi ljudi predstavljaju direktnu posledicu intezivnog kontakta sa
životinjama, pri čemu najmanje 75% novih infektivnih i pretećih infektivnih bolesti pripada
zoonozama ili vektorski prenosivim bolestima. Kućni ljubimci na različite načine ljudima mogu
preneti veliki broj infektivnih oboljenja. U najznačajnije zoonoze se svrstavaju infekcije izazvane
virusom besnila, norovirusima, rotavirusima ili virusima influence, kao i veliki broj bakterijskih
infekcija kao što su salmoneloza, kampilobakterioza, bruceloza i leptospiroza. Bakterijske
zoonoze ukjlučuju i bakterije koje se prenose ujedom ili putem ogrebotina kao što su Pasteurella
multocida ili Bartonella henselae. Sve veći značaj pridaje se transmisiji meticilin rezistentnih
sojeva Staphylococcus aureus (MRSA) između ljudi i životinja, a dokazan je i porast prevalencije
ovih bakterija u izolatima poreklom iz kućnih ljubimaca. Ne treba zanemariti ni činjenicu da psi i
mačke takođe mogu biti izvor gljivičnih infekcija kao što su sporotrihoza ili dermatofitoza. U
najznačajnije zoonotske patogene parazitske etiologije spadaju uzročnici toksoplazmoze,
đardioze, toksokarioze, onhocerkoze i ehinokokoze. Pored toga, sa globalnim otopljavanjem sve
su rasprostranjenije i vektorski prenosive zoonoze izazvane sa: Anaplasma phagocytophilum,
Rickettsia felis, Borrelia burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis
i Dirofilaria repens koje se putem krpelja, buva, peščanih mušica i komaraca prenose sa kućnih
ljubimaca na ljude. S obzirom da veliki broj pomenutih zoonoza predstavlja pretnju po javno
zdravlje, neophodno je pristupiti ovom problemu sa dve strane. Veterinari moraju upoznati
vlasnike sa metodama prevencije infektivnih bolesti kućnih ljubimaca, a koje podrazumevaju
odgovorno vlasništvo. S druge strane, lekari moraju imati u vidu mogućnost pojave zoonoza i
savetovati pacijente u cilju sprečavanja infekcije ljudi. Primenom koncepta jedinstvenog zdravlja
neophodno je uspostavljanje bliže saradnje lekara i veterinara, a jačanjem epidemioloških i
laboratorijskih ispitivanja moguće je utvrđivanje prisustva, raširenosti i rizika od pojave zoonoza
ljudi poreklom od kućnih ljubimaca, a zajedničkim radom mogu se razviti i primeniti održive i
efektivne mere zaštite zdravlja ljudi i životinja., Many emerging health issues are linked to increasing contact between humans and animals, and
it is estimated that at least 75% of emerging and re-emerging diseases are either zoonotic or
vector-borne. Pets may transmit a variety of infectious diseases to humans. Viral diseases caused
by rabies virus, noroviruses, rotaviruses or influenza viruses, as well as numerous bacterial
infections such as salmonellosis, campylobacteriosis, brucellosis and leptospirosis present some
of the most significant zoonoses linked to household pets. Bacterial zoonoses also include bacteria
transmitted by bites or scratches like Pasteurella multocida or Bartonella henselae. More attention
is paid to the transmission of methicilin resistant strains of Staphylococcus aureus (MRSA)
between humans and animals, since the prevalence of these isolates from samples of animal origin
is on the rise. Moreover, the fact that dogs and cats can be sources of serious fungal infections such
as sporotrichosis or dermatophytosis shouldn’t be overseen. Most important zoonotic parasites
are the causative agents of toxoplasmosis, giardiasis, toxocariasis, onchocercosis and
echinococcosis. One of the apparent consequences of global warming is the increased prevalence
of vector borne zoonotic diseases caused by: Anaplasma phagocytophilum, Rickettsia felis, Borrelia
burgdorferi, Dipylidium caninum, Leishmania infantum, Dirofilaria immitis and Dirofilaria repens
transmitted from animals to humans by ticks, fleas, phlebotomine sand flies and mosquitoes.
Considering that many of the mentioned zoonoses pose a serious risk to public health, it is
essential to address this problem from two sides. Veterinarians must instruct the owners on
necessary preventive measures implying responsible pet ownership. On the other hand,
physicians must maintain awareness of pet infectious diseases and counsel patients to prevent
human infection. By strengthening epidemiologic and laboratory investigations that assess the
role of environmental influences, this partnership can help develop and apply sustainable and
effective community health interventions.",
publisher = "Udruženje mikrobiologa Srbije, Beograd",
journal = "XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO",
title = "Kućni ljubimci i zoonoze, Pets and zoonoses",
pages = "153-154",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2265"
}

Radojičić, M., Marković, M., Milić, N., Kulišić, Z., Radalj, A.,& Krnjaić, D.. (2018). Kućni ljubimci i zoonoze. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
Udruženje mikrobiologa Srbije, Beograd., 153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2265

Radojičić M, Marković M, Milić N, Kulišić Z, Radalj A, Krnjaić D. Kućni ljubimci i zoonoze. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO. 2018;:153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2265 .

Radojičić, Marina, Marković, Maja, Milić, Nenad, Kulišić, Zoran, Radalj, Andrea, Krnjaić, Dejan, "Kućni ljubimci i zoonoze" in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO (2018):153-154,
https://hdl.handle.net/21.15107/rcub_veterinar_2265 .

TY  - JOUR
AU  - Gavrilović, A.
AU  - Gavrilović, P.
AU  - Radojičić, Sonja
AU  - Krnjaić, Dejan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1570
AB  - Bluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohens kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays
VL  - 69
IS  - 3
SP  - 1088
EP  - 1093
DO  - 10.12681/jhvms.18880
ER  - 

@article{
author = "Gavrilović, A. and Gavrilović, P. and Radojičić, Sonja and Krnjaić, Dejan",
year = "2018",
abstract = "Bluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohens kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays",
volume = "69",
number = "3",
pages = "1088-1093",
doi = "10.12681/jhvms.18880"
}

Gavrilović, A., Gavrilović, P., Radojičić, S.,& Krnjaić, D.. (2018). Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(3), 1088-1093.
https://doi.org/10.12681/jhvms.18880

Gavrilović A, Gavrilović P, Radojičić S, Krnjaić D. Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays. in Journal of The Hellenic Veterinary Medical Society. 2018;69(3):1088-1093.
doi:10.12681/jhvms.18880 .

Gavrilović, A., Gavrilović, P., Radojičić, Sonja, Krnjaić, Dejan, "Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays" in Journal of The Hellenic Veterinary Medical Society, 69, no. 3 (2018):1088-1093,
https://doi.org/10.12681/jhvms.18880 . .

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Grego, Edita
AU  - Vidanović, Dejan
AU  - Milanov, Dubravka
AU  - Krnjaić, Dejan
AU  - Kehrenberg, Corinna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1648
AB  - The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
VL  - 24
IS  - 1
SP  - 95
EP  - 103
DO  - 10.1089/mdr.2017.0016
ER  - 

@article{
author = "Todorović, Dalibor and Velhner, Maja and Grego, Edita and Vidanović, Dejan and Milanov, Dubravka and Krnjaić, Dejan and Kehrenberg, Corinna",
year = "2018",
abstract = "The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia",
volume = "24",
number = "1",
pages = "95-103",
doi = "10.1089/mdr.2017.0016"
}

Todorović, D., Velhner, M., Grego, E., Vidanović, D., Milanov, D., Krnjaić, D.,& Kehrenberg, C.. (2018). Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 24(1), 95-103.
https://doi.org/10.1089/mdr.2017.0016

Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance. 2018;24(1):95-103.
doi:10.1089/mdr.2017.0016 .

Todorović, Dalibor, Velhner, Maja, Grego, Edita, Vidanović, Dejan, Milanov, Dubravka, Krnjaić, Dejan, Kehrenberg, Corinna, "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia" in Microbial Drug Resistance, 24, no. 1 (2018):95-103,
https://doi.org/10.1089/mdr.2017.0016 . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1453
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Lukač, Bojan
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Đurić, Spomenka
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1352
AB  - The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.
AB  - Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina
T1  - Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina
VL  - 66
IS  - 1
SP  - 51
EP  - 60
DO  - 10.1515/acve-2016-0004
ER  - 

@article{
author = "Lukač, Bojan and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Đurić, Spomenka and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China., Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina, Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina",
volume = "66",
number = "1",
pages = "51-60",
doi = "10.1515/acve-2016-0004"
}

Lukač, B., Knežević, A., Milić, N., Krnjaić, D., Veljović, L., Milićević, V., Zorić, A., Đurić, S., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 51-60.
https://doi.org/10.1515/acve-2016-0004

Lukač B, Knežević A, Milić N, Krnjaić D, Veljović L, Milićević V, Zorić A, Đurić S, Stanojević M, Nišavić J. Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd. 2016;66(1):51-60.
doi:10.1515/acve-2016-0004 .

Lukač, Bojan, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Đurić, Spomenka, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina" in Acta Veterinaria-Beograd, 66, no. 1 (2016):51-60,
https://doi.org/10.1515/acve-2016-0004 . .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .