TY  - JOUR
AU  - Birinji, Anita
AU  - Lalošević, Dušan
AU  - Mihaljev, Željko
AU  - Pogrmić-Majkić, Kristina
AU  - Radovanović, Anita
AU  - Glišić, Radmila
AU  - Marin, Marija
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3861
AB  - This study aimed to measure the total arsenic content deposited in the ovaries of
three consecutive generations of mice. The animals were treated with two different
concentrations to determine whether histological changes were caused in the ovaries.
The control group of mice received tap water, whereas the experimental groups were
given different concentrations of dissolved arsenic (III)-oxide. The arsenic content in
the ovaries in both experimental groups increased with each generation. The highest
content was recorded in the third generation of the second experimental group. Between
the two experimental groups in each generation, significant differences in the average
number of corpora lutea and ovarian follicles were identified. Arsenic caused structural
changes in the ovaries in both experimental groups in all three consecutive generations.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Impact of arsenic on mouse ovaries over three generations
VL  - 74
IS  - 1
SP  - 60
EP  - 70
DO  - 10.2478/acve-2024-0005
ER  - 

@article{
author = "Birinji, Anita and Lalošević, Dušan and Mihaljev, Željko and Pogrmić-Majkić, Kristina and Radovanović, Anita and Glišić, Radmila and Marin, Marija",
year = "2024",
abstract = "This study aimed to measure the total arsenic content deposited in the ovaries of
three consecutive generations of mice. The animals were treated with two different
concentrations to determine whether histological changes were caused in the ovaries.
The control group of mice received tap water, whereas the experimental groups were
given different concentrations of dissolved arsenic (III)-oxide. The arsenic content in
the ovaries in both experimental groups increased with each generation. The highest
content was recorded in the third generation of the second experimental group. Between
the two experimental groups in each generation, significant differences in the average
number of corpora lutea and ovarian follicles were identified. Arsenic caused structural
changes in the ovaries in both experimental groups in all three consecutive generations.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Impact of arsenic on mouse ovaries over three generations",
volume = "74",
number = "1",
pages = "60-70",
doi = "10.2478/acve-2024-0005"
}

Birinji, A., Lalošević, D., Mihaljev, Ž., Pogrmić-Majkić, K., Radovanović, A., Glišić, R.,& Marin, M.. (2024). Impact of arsenic on mouse ovaries over three generations. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 60-70.
https://doi.org/10.2478/acve-2024-0005

Birinji A, Lalošević D, Mihaljev Ž, Pogrmić-Majkić K, Radovanović A, Glišić R, Marin M. Impact of arsenic on mouse ovaries over three generations. in Acta Veterinaria. 2024;74(1):60-70.
doi:10.2478/acve-2024-0005 .

Birinji, Anita, Lalošević, Dušan, Mihaljev, Željko, Pogrmić-Majkić, Kristina, Radovanović, Anita, Glišić, Radmila, Marin, Marija, "Impact of arsenic on mouse ovaries over three generations" in Acta Veterinaria, 74, no. 1 (2024):60-70,
https://doi.org/10.2478/acve-2024-0005 . .

TY  - JOUR
AU  - Horyanto, Darwin
AU  - Bajagai, Yadav S.
AU  - Kayal, Advait
AU  - von Hellens, Juhani
AU  - Chen, Xiaojing
AU  - Van, Thi Thu Hao
AU  - Radovanović, Anita
AU  - Stanley, Dragana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3700
AB  - The supplementation of antimicrobial growth promoters (AGPs) has been banned in many
countries because of the emergence of antimicrobial-resistant pathogens in poultry products and
the environment. Probiotics have been broadly studied and demonstrated as a promising AGP
substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product
on broiler production performance and gut microbial profile in a dexamethasone-induced leaky
gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into
four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented
with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing
a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)).
The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota
were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved
production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on
microbiota was observed in other gut sections.
PB  - MDPI
T2  - Microorganisms
T1  - Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model
VL  - 12
IS  - 2
SP  - 419
DO  - 10.3390/microorganisms12020419
ER  - 

@article{
author = "Horyanto, Darwin and Bajagai, Yadav S. and Kayal, Advait and von Hellens, Juhani and Chen, Xiaojing and Van, Thi Thu Hao and Radovanović, Anita and Stanley, Dragana",
year = "2024",
abstract = "The supplementation of antimicrobial growth promoters (AGPs) has been banned in many
countries because of the emergence of antimicrobial-resistant pathogens in poultry products and
the environment. Probiotics have been broadly studied and demonstrated as a promising AGP
substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product
on broiler production performance and gut microbial profile in a dexamethasone-induced leaky
gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into
four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented
with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing
a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)).
The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota
were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved
production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on
microbiota was observed in other gut sections.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model",
volume = "12",
number = "2",
pages = "419",
doi = "10.3390/microorganisms12020419"
}

Horyanto, D., Bajagai, Y. S., Kayal, A., von Hellens, J., Chen, X., Van, T. T. H., Radovanović, A.,& Stanley, D.. (2024). Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model. in Microorganisms
MDPI., 12(2), 419.
https://doi.org/10.3390/microorganisms12020419

Horyanto D, Bajagai YS, Kayal A, von Hellens J, Chen X, Van TTH, Radovanović A, Stanley D. Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model. in Microorganisms. 2024;12(2):419.
doi:10.3390/microorganisms12020419 .

Horyanto, Darwin, Bajagai, Yadav S., Kayal, Advait, von Hellens, Juhani, Chen, Xiaojing, Van, Thi Thu Hao, Radovanović, Anita, Stanley, Dragana, "Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model" in Microorganisms, 12, no. 2 (2024):419,
https://doi.org/10.3390/microorganisms12020419 . .

TY  - CONF
AU  - Milošević, Ivan
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Nikolić, Anja
AU  - Lužajić Božinovski, Tijana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2656
AB  - Svrha ovog pregleda je da prikaže veliku raznolikost u strukturi i funkciji placente koja se sreće kod sisara, i podobnosti nekih vrsta kao animalnih modela u proučavanju placentacije. Postoje značajne razlike u strukturi i fiziologiji posteljice između čoveka, domaćih i eksperimentalnih životinja. Zbog toga, najčešće upotrebljavani animalni modeli za proučavanje placentacije (miš, kunić, zamorac), nisu adekvatni za sva istraživanja u veterinarskoj i humanoj medicini. Placente se klasifikuju prema svom obliku, strukturi i interhemalnom regionu. Horionske resice placente su obložene prizmatičnim epitelom i preko njega se odvija prenos svih supstanci od majke prema plodu. Ćelije ovog epitela su selektivno propustljive kako za korisne, tako i za potencijalno toksične materije.
	Istraživanja koja se bave analizama i osobenostima placentacije su oskudna, posebno sa aspekta kontakta i razmene materija između majke i ploda. Iz tog razloga, ovaj uvid može da pomogne u sagledavanju različitih problema u kliničkoj praksi i tretmanu koji su bitni za dobijanje zdravog potomstva.
PB  - Fakultet veterinarske medicine, Beograd, Centar za izdavačku delatnost i promet učila
C3  - 44. Seminar za inovacije znanja veterinara, Beograd, 24.02.2023.
T1  - Komparativni prikaz animalnih modela u morfološkim analizama placentacije
SP  - 73
EP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2656
ER  - 

@conference{
author = "Milošević, Ivan and Marković, Danica and Radovanović, Anita and Nikolić, Anja and Lužajić Božinovski, Tijana",
year = "2023",
abstract = "Svrha ovog pregleda je da prikaže veliku raznolikost u strukturi i funkciji placente koja se sreće kod sisara, i podobnosti nekih vrsta kao animalnih modela u proučavanju placentacije. Postoje značajne razlike u strukturi i fiziologiji posteljice između čoveka, domaćih i eksperimentalnih životinja. Zbog toga, najčešće upotrebljavani animalni modeli za proučavanje placentacije (miš, kunić, zamorac), nisu adekvatni za sva istraživanja u veterinarskoj i humanoj medicini. Placente se klasifikuju prema svom obliku, strukturi i interhemalnom regionu. Horionske resice placente su obložene prizmatičnim epitelom i preko njega se odvija prenos svih supstanci od majke prema plodu. Ćelije ovog epitela su selektivno propustljive kako za korisne, tako i za potencijalno toksične materije.
	Istraživanja koja se bave analizama i osobenostima placentacije su oskudna, posebno sa aspekta kontakta i razmene materija između majke i ploda. Iz tog razloga, ovaj uvid može da pomogne u sagledavanju različitih problema u kliničkoj praksi i tretmanu koji su bitni za dobijanje zdravog potomstva.",
publisher = "Fakultet veterinarske medicine, Beograd, Centar za izdavačku delatnost i promet učila",
journal = "44. Seminar za inovacije znanja veterinara, Beograd, 24.02.2023.",
title = "Komparativni prikaz animalnih modela u morfološkim analizama placentacije",
pages = "73-83",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2656"
}

Milošević, I., Marković, D., Radovanović, A., Nikolić, A.,& Lužajić Božinovski, T.. (2023). Komparativni prikaz animalnih modela u morfološkim analizama placentacije. in 44. Seminar za inovacije znanja veterinara, Beograd, 24.02.2023.
Fakultet veterinarske medicine, Beograd, Centar za izdavačku delatnost i promet učila., 73-83.
https://hdl.handle.net/21.15107/rcub_veterinar_2656

Milošević I, Marković D, Radovanović A, Nikolić A, Lužajić Božinovski T. Komparativni prikaz animalnih modela u morfološkim analizama placentacije. in 44. Seminar za inovacije znanja veterinara, Beograd, 24.02.2023.. 2023;:73-83.
https://hdl.handle.net/21.15107/rcub_veterinar_2656 .

Milošević, Ivan, Marković, Danica, Radovanović, Anita, Nikolić, Anja, Lužajić Božinovski, Tijana, "Komparativni prikaz animalnih modela u morfološkim analizama placentacije" in 44. Seminar za inovacije znanja veterinara, Beograd, 24.02.2023. (2023):73-83,
https://hdl.handle.net/21.15107/rcub_veterinar_2656 .

TY  - JOUR
AU  - Lužajić Božinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Nikolić, Anja
AU  - Radovanović, Anita
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Vasić, Mirjana
AU  - Milošević, Ivan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3047
AB  - Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives
VL  - 73
IS  - 2
SP  - 231
EP  - 248
DO  - 10.2478/acve-2023-0018
ER  - 

@article{
author = "Lužajić Božinovski, Tijana and Danilović Luković, Jelena and Nikolić, Anja and Radovanović, Anita and Marković, Danica and Kovačević Filipović, Milica and Vasić, Mirjana and Milošević, Ivan",
year = "2023",
abstract = "Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives",
volume = "73",
number = "2",
pages = "231-248",
doi = "10.2478/acve-2023-0018"
}

Lužajić Božinovski, T., Danilović Luković, J., Nikolić, A., Radovanović, A., Marković, D., Kovačević Filipović, M., Vasić, M.,& Milošević, I.. (2023). Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 73(2), 231-248.
https://doi.org/10.2478/acve-2023-0018

Lužajić Božinovski T, Danilović Luković J, Nikolić A, Radovanović A, Marković D, Kovačević Filipović M, Vasić M, Milošević I. Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives. in Acta Veterinaria. 2023;73(2):231-248.
doi:10.2478/acve-2023-0018 .

Lužajić Božinovski, Tijana, Danilović Luković, Jelena, Nikolić, Anja, Radovanović, Anita, Marković, Danica, Kovačević Filipović, Milica, Vasić, Mirjana, Milošević, Ivan, "Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives" in Acta Veterinaria, 73, no. 2 (2023):231-248,
https://doi.org/10.2478/acve-2023-0018 . .

TY  - CONF
AU  - Nikolić, Anja
AU  - Milošević, Ivan
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Prokić, Bogomir Bolka
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Mišković-Stanković, Vesna
AU  - Lužajić Božinovski, Tijana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3389
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 10th International Congress Veterinary Science and Profession, Zagreb, October 5th - 7th, 2023
T1  - Clinical evaluation of the effects of novel Polyvinyl Alcohol/Gentamicin (PVA/Gent) and Polyvinyl Alcohol/Chitosan/Gentamicin (PVA/CHI/Gent) hydrogels on the healing of second-degree burn wounds using a rat animal model
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3389
ER  - 

@conference{
author = "Nikolić, Anja and Milošević, Ivan and Janković, Ana and Stevanović, Milena and Prokić, Bogomir Bolka and Marković, Danica and Radovanović, Anita and Mišković-Stanković, Vesna and Lužajić Božinovski, Tijana",
year = "2023",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "10th International Congress Veterinary Science and Profession, Zagreb, October 5th - 7th, 2023",
title = "Clinical evaluation of the effects of novel Polyvinyl Alcohol/Gentamicin (PVA/Gent) and Polyvinyl Alcohol/Chitosan/Gentamicin (PVA/CHI/Gent) hydrogels on the healing of second-degree burn wounds using a rat animal model",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3389"
}

Nikolić, A., Milošević, I., Janković, A., Stevanović, M., Prokić, B. B., Marković, D., Radovanović, A., Mišković-Stanković, V.,& Lužajić Božinovski, T.. (2023). Clinical evaluation of the effects of novel Polyvinyl Alcohol/Gentamicin (PVA/Gent) and Polyvinyl Alcohol/Chitosan/Gentamicin (PVA/CHI/Gent) hydrogels on the healing of second-degree burn wounds using a rat animal model. in 10th International Congress Veterinary Science and Profession, Zagreb, October 5th - 7th, 2023
Faculty of Veterinary Medicine, University of Zagreb., 71-71.
https://hdl.handle.net/21.15107/rcub_veterinar_3389

Nikolić A, Milošević I, Janković A, Stevanović M, Prokić BB, Marković D, Radovanović A, Mišković-Stanković V, Lužajić Božinovski T. Clinical evaluation of the effects of novel Polyvinyl Alcohol/Gentamicin (PVA/Gent) and Polyvinyl Alcohol/Chitosan/Gentamicin (PVA/CHI/Gent) hydrogels on the healing of second-degree burn wounds using a rat animal model. in 10th International Congress Veterinary Science and Profession, Zagreb, October 5th - 7th, 2023. 2023;:71-71.
https://hdl.handle.net/21.15107/rcub_veterinar_3389 .

Nikolić, Anja, Milošević, Ivan, Janković, Ana, Stevanović, Milena, Prokić, Bogomir Bolka, Marković, Danica, Radovanović, Anita, Mišković-Stanković, Vesna, Lužajić Božinovski, Tijana, "Clinical evaluation of the effects of novel Polyvinyl Alcohol/Gentamicin (PVA/Gent) and Polyvinyl Alcohol/Chitosan/Gentamicin (PVA/CHI/Gent) hydrogels on the healing of second-degree burn wounds using a rat animal model" in 10th International Congress Veterinary Science and Profession, Zagreb, October 5th - 7th, 2023 (2023):71-71,
https://hdl.handle.net/21.15107/rcub_veterinar_3389 .

TY  - JOUR
AU  - Maksimović, Željko
AU  - Starčević, Marija
AU  - Šefer, Dragan
AU  - Janjić, Jelena
AU  - Radovanović, Anita
AU  - Radulović, Stamen
AU  - Perić, Dejan
AU  - Marković, Radmila
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2869
AB  - Th e aim of this study was to evaluate the eff ect of adding diff erent dosages of live yeast (Saccharomyces cerevisiae) in diet on
growth performance, carcass characteristics, intestinal morphology, and gut microfl ora of broilers. Th e study was conducted on 270 oneday old chickens (Ross 308) during a 42-day period. Broilers were randomly allocated to one of three dietary treatments that diff ered in
the content of added yeast: no additional yeast; with 0.25 g/kg; and with 0.65 g/kg of added yeast. Each experimental group contained 90
animals. Th e results from our study showed that diet supplemented with 0.25 g/kg of yeast resulted in better growth performance and
carcass quality (P<0.05), improved intestinal morphology (P<0.001), higher development of beneficial microfl ora (Lactobacillus spp.)
(P<0.01), and reduction of pathogenic bacteria (Escherichia coli) in guts of broilers than in the group fed only with the basic diet (P<0.05).
On the other hand, the group with the highest inclusion of yeast in diet (0.65 g/kg) achieved similar results for the examined parameters
as did the group with no added yeast. Th erefore, the adequate dose of S. cerevisiae in diet was 0.25 g/kg of yeast, while higher dose of live
yeast in broiler diet (0.65 g/kg) did not achieve better results.
PB  - Kafkas University, Faculty of Veterinary Medicine
T2  - Kafkas Universitesi Veteriner Fakultesi Dergisi
T1  - Effects of Adding Different Dosages of Saccharomyces cerevisiae in Diet on Growth Performance, Carcass Characteristics, Intestinal Morphology, and Gut Microflora of Broilers
VL  - 28
IS  - 4
SP  - 461
EP  - 468
DO  - 10.9775/kvfd.2022.27458
ER  - 

@article{
author = "Maksimović, Željko and Starčević, Marija and Šefer, Dragan and Janjić, Jelena and Radovanović, Anita and Radulović, Stamen and Perić, Dejan and Marković, Radmila",
year = "2022",
abstract = "Th e aim of this study was to evaluate the eff ect of adding diff erent dosages of live yeast (Saccharomyces cerevisiae) in diet on
growth performance, carcass characteristics, intestinal morphology, and gut microfl ora of broilers. Th e study was conducted on 270 oneday old chickens (Ross 308) during a 42-day period. Broilers were randomly allocated to one of three dietary treatments that diff ered in
the content of added yeast: no additional yeast; with 0.25 g/kg; and with 0.65 g/kg of added yeast. Each experimental group contained 90
animals. Th e results from our study showed that diet supplemented with 0.25 g/kg of yeast resulted in better growth performance and
carcass quality (P<0.05), improved intestinal morphology (P<0.001), higher development of beneficial microfl ora (Lactobacillus spp.)
(P<0.01), and reduction of pathogenic bacteria (Escherichia coli) in guts of broilers than in the group fed only with the basic diet (P<0.05).
On the other hand, the group with the highest inclusion of yeast in diet (0.65 g/kg) achieved similar results for the examined parameters
as did the group with no added yeast. Th erefore, the adequate dose of S. cerevisiae in diet was 0.25 g/kg of yeast, while higher dose of live
yeast in broiler diet (0.65 g/kg) did not achieve better results.",
publisher = "Kafkas University, Faculty of Veterinary Medicine",
journal = "Kafkas Universitesi Veteriner Fakultesi Dergisi",
title = "Effects of Adding Different Dosages of Saccharomyces cerevisiae in Diet on Growth Performance, Carcass Characteristics, Intestinal Morphology, and Gut Microflora of Broilers",
volume = "28",
number = "4",
pages = "461-468",
doi = "10.9775/kvfd.2022.27458"
}

Maksimović, Ž., Starčević, M., Šefer, D., Janjić, J., Radovanović, A., Radulović, S., Perić, D.,& Marković, R.. (2022). Effects of Adding Different Dosages of Saccharomyces cerevisiae in Diet on Growth Performance, Carcass Characteristics, Intestinal Morphology, and Gut Microflora of Broilers. in Kafkas Universitesi Veteriner Fakultesi Dergisi
Kafkas University, Faculty of Veterinary Medicine., 28(4), 461-468.
https://doi.org/10.9775/kvfd.2022.27458

Maksimović Ž, Starčević M, Šefer D, Janjić J, Radovanović A, Radulović S, Perić D, Marković R. Effects of Adding Different Dosages of Saccharomyces cerevisiae in Diet on Growth Performance, Carcass Characteristics, Intestinal Morphology, and Gut Microflora of Broilers. in Kafkas Universitesi Veteriner Fakultesi Dergisi. 2022;28(4):461-468.
doi:10.9775/kvfd.2022.27458 .

Maksimović, Željko, Starčević, Marija, Šefer, Dragan, Janjić, Jelena, Radovanović, Anita, Radulović, Stamen, Perić, Dejan, Marković, Radmila, "Effects of Adding Different Dosages of Saccharomyces cerevisiae in Diet on Growth Performance, Carcass Characteristics, Intestinal Morphology, and Gut Microflora of Broilers" in Kafkas Universitesi Veteriner Fakultesi Dergisi, 28, no. 4 (2022):461-468,
https://doi.org/10.9775/kvfd.2022.27458 . .

TY  - JOUR
AU  - Đorđević, Jasna
AU  - Baltić, Branislav
AU  - Glišić, Milica
AU  - Bošković, Marija
AU  - Šefer, Dragan
AU  - Radulović, Stamen
AU  - Radovanović, Anita
AU  - Perić, Dejan
AU  - Peurača, Mile
AU  - Marković, Radmila
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2571
AB  - The aim was to examine production performance, carcass characteristics, and intestinal histomorphology of weaned piglets (28-to 54-day-old) fed diet with 3 or 5 g of sodium butyrate per kg of diet (group II and III). Groups II and III had higher final live weight and total weight gain. The feed to gain ratio was the best in group I. The highest carcass yield was of group III. Significant differences (P<0.05) were observed in Escherischia coli counts in small intestine between control (I) and experimental groups (II and III), and in cecum between control (I) and experimental group II. There were significant differences (P<0.05) between all groups for the intestine length, intestine weight, and both height and width of the ileal villus. The highest villus height/crypt depth ratio of jejunum occurred in group II piglets, while the highest villus height/crypt depth ratio of ileum was in piglets from group III. The significant correlations were determined between amount of sodium butyrate and final live weight, intestinal length, intestinal weight, jejunal and ileal crypt depth, and ileal villus widths.
PB  - Animal Nutrition Association
T2  - Animal Nutrition and Feed Technology
T1  - Advantages of sodium butyrate in weaned piglet diet
VL  - 22
IS  - 2
SP  - 245
EP  - 260
DO  - 10.5958/0974-181X.2022.00020.8
ER  - 

@article{
author = "Đorđević, Jasna and Baltić, Branislav and Glišić, Milica and Bošković, Marija and Šefer, Dragan and Radulović, Stamen and Radovanović, Anita and Perić, Dejan and Peurača, Mile and Marković, Radmila",
year = "2022",
abstract = "The aim was to examine production performance, carcass characteristics, and intestinal histomorphology of weaned piglets (28-to 54-day-old) fed diet with 3 or 5 g of sodium butyrate per kg of diet (group II and III). Groups II and III had higher final live weight and total weight gain. The feed to gain ratio was the best in group I. The highest carcass yield was of group III. Significant differences (P<0.05) were observed in Escherischia coli counts in small intestine between control (I) and experimental groups (II and III), and in cecum between control (I) and experimental group II. There were significant differences (P<0.05) between all groups for the intestine length, intestine weight, and both height and width of the ileal villus. The highest villus height/crypt depth ratio of jejunum occurred in group II piglets, while the highest villus height/crypt depth ratio of ileum was in piglets from group III. The significant correlations were determined between amount of sodium butyrate and final live weight, intestinal length, intestinal weight, jejunal and ileal crypt depth, and ileal villus widths.",
publisher = "Animal Nutrition Association",
journal = "Animal Nutrition and Feed Technology",
title = "Advantages of sodium butyrate in weaned piglet diet",
volume = "22",
number = "2",
pages = "245-260",
doi = "10.5958/0974-181X.2022.00020.8"
}

Đorđević, J., Baltić, B., Glišić, M., Bošković, M., Šefer, D., Radulović, S., Radovanović, A., Perić, D., Peurača, M.,& Marković, R.. (2022). Advantages of sodium butyrate in weaned piglet diet. in Animal Nutrition and Feed Technology
Animal Nutrition Association., 22(2), 245-260.
https://doi.org/10.5958/0974-181X.2022.00020.8

Đorđević J, Baltić B, Glišić M, Bošković M, Šefer D, Radulović S, Radovanović A, Perić D, Peurača M, Marković R. Advantages of sodium butyrate in weaned piglet diet. in Animal Nutrition and Feed Technology. 2022;22(2):245-260.
doi:10.5958/0974-181X.2022.00020.8 .

Đorđević, Jasna, Baltić, Branislav, Glišić, Milica, Bošković, Marija, Šefer, Dragan, Radulović, Stamen, Radovanović, Anita, Perić, Dejan, Peurača, Mile, Marković, Radmila, "Advantages of sodium butyrate in weaned piglet diet" in Animal Nutrition and Feed Technology, 22, no. 2 (2022):245-260,
https://doi.org/10.5958/0974-181X.2022.00020.8 . .

TY  - JOUR
AU  - Kayal, Advait
AU  - Stanley, Dragana
AU  - Radovanović, Anita
AU  - Horyanto, Darwin
AU  - Thi Thu, Thi Thu
AU  - Bajagai, Yadav S.
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2795
AB  - The concept of designer microbiota in chicken is focused on early exposure of the hatchlings
to pathogen-free microbiota inoculum, limiting the early access to harmful and pathogenic
microorganisms, thus promoting colonisation of the gut with beneficial and natural poultry microbiota.
In this study, we controlled colonisation of the intestine in broiler chickens in a large-scale
industrial setting via at-hatch administration of a commercial product containing a highly diverse
microbiota originating from the chicken caecum. The treatment significantly transformed the microbiota
membership in the crop, proventriculus, jejunum and caecum and significantly altered the
taxa abundance in the jejunum, jejunum mucosa, and caecum estimated using PERMANOVA and
unweighted and weighted UniFrac distances, respectively. The treatment also improved the growth
rate in chickens with no significant alteration in feed conversion ratio. A comparison of inoculum
product microbiota structure revealed that the inoculum had the highest Shannon diversity index
compared to all investigated gut sections, and the number of Observed Species second only to the
caecal community. PCoA plots using weighted or unweighted UniFrac placed the inoculum samples
together with the samples from the caecal origin.
PB  - MDPI
T2  - Animals
T1  - Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System
VL  - 12
SP  - 3296
DO  - 10.3390/ani12233296
ER  - 

@article{
author = "Kayal, Advait and Stanley, Dragana and Radovanović, Anita and Horyanto, Darwin and Thi Thu, Thi Thu and Bajagai, Yadav S.",
year = "2022",
abstract = "The concept of designer microbiota in chicken is focused on early exposure of the hatchlings
to pathogen-free microbiota inoculum, limiting the early access to harmful and pathogenic
microorganisms, thus promoting colonisation of the gut with beneficial and natural poultry microbiota.
In this study, we controlled colonisation of the intestine in broiler chickens in a large-scale
industrial setting via at-hatch administration of a commercial product containing a highly diverse
microbiota originating from the chicken caecum. The treatment significantly transformed the microbiota
membership in the crop, proventriculus, jejunum and caecum and significantly altered the
taxa abundance in the jejunum, jejunum mucosa, and caecum estimated using PERMANOVA and
unweighted and weighted UniFrac distances, respectively. The treatment also improved the growth
rate in chickens with no significant alteration in feed conversion ratio. A comparison of inoculum
product microbiota structure revealed that the inoculum had the highest Shannon diversity index
compared to all investigated gut sections, and the number of Observed Species second only to the
caecal community. PCoA plots using weighted or unweighted UniFrac placed the inoculum samples
together with the samples from the caecal origin.",
publisher = "MDPI",
journal = "Animals",
title = "Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System",
volume = "12",
pages = "3296",
doi = "10.3390/ani12233296"
}

Kayal, A., Stanley, D., Radovanović, A., Horyanto, D., Thi Thu, T. T.,& Bajagai, Y. S.. (2022). Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System. in Animals
MDPI., 12, 3296.
https://doi.org/10.3390/ani12233296

Kayal A, Stanley D, Radovanović A, Horyanto D, Thi Thu TT, Bajagai YS. Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System. in Animals. 2022;12:3296.
doi:10.3390/ani12233296 .

Kayal, Advait, Stanley, Dragana, Radovanović, Anita, Horyanto, Darwin, Thi Thu, Thi Thu, Bajagai, Yadav S., "Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System" in Animals, 12 (2022):3296,
https://doi.org/10.3390/ani12233296 . .

TY  - JOUR
AU  - Bajagai, Yadav S.
AU  - Radovanović, Anita
AU  - Steel, Jason C.
AU  - Stanley, Dragana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1995
AB  - Pathogen control is re-emerging as a significant challenge to the health of both humans and animals. The livestock industry is in the process of massively replacing in-feed antibiotics with organic production friendly plant-based products. Nutrigenomics as a science of the effects of food constituents on gene expression is shedding more light on both benefits and detrimental side-effects of feed additive prolonged consumption on the host, indicating the need to understand the feed– host interactions and their influence on the host disease profile. In this study, we investigated the effects of 2% oregano powder supplementation on the liver gene expression in healthy male broilers from the hatch to 6 weeks of age. Deep RNAseq was performed on average 113.3 million paired and quality trimmed sequences per sample and four samples for the control and treatment each. The results demonstrate the severity of oregano effect on liver gene expression with substantial modifications in steroid hormone regulation, fat and carbohydrate metabolism alterations and strong influence on the host disease and function profile. Oregano supplementation was able to interfere with the transcriptional effects of a range of registered drugs and to significantly transcriptionally inhibit a range of cancer disease categories including liver cancer, and to modify fat and carbohydrate metabolism.
PB  - MDPI AG
T2  - Animals
T1  - The effects of continual consumption of origanum vulgare on liver transcriptomics
VL  - 11
IS  - 2
SP  - 1
EP  - 16
DO  - 10.3390/ani11020398
ER  - 

@article{
author = "Bajagai, Yadav S. and Radovanović, Anita and Steel, Jason C. and Stanley, Dragana",
year = "2021",
abstract = "Pathogen control is re-emerging as a significant challenge to the health of both humans and animals. The livestock industry is in the process of massively replacing in-feed antibiotics with organic production friendly plant-based products. Nutrigenomics as a science of the effects of food constituents on gene expression is shedding more light on both benefits and detrimental side-effects of feed additive prolonged consumption on the host, indicating the need to understand the feed– host interactions and their influence on the host disease profile. In this study, we investigated the effects of 2% oregano powder supplementation on the liver gene expression in healthy male broilers from the hatch to 6 weeks of age. Deep RNAseq was performed on average 113.3 million paired and quality trimmed sequences per sample and four samples for the control and treatment each. The results demonstrate the severity of oregano effect on liver gene expression with substantial modifications in steroid hormone regulation, fat and carbohydrate metabolism alterations and strong influence on the host disease and function profile. Oregano supplementation was able to interfere with the transcriptional effects of a range of registered drugs and to significantly transcriptionally inhibit a range of cancer disease categories including liver cancer, and to modify fat and carbohydrate metabolism.",
publisher = "MDPI AG",
journal = "Animals",
title = "The effects of continual consumption of origanum vulgare on liver transcriptomics",
volume = "11",
number = "2",
pages = "1-16",
doi = "10.3390/ani11020398"
}

Bajagai, Y. S., Radovanović, A., Steel, J. C.,& Stanley, D.. (2021). The effects of continual consumption of origanum vulgare on liver transcriptomics. in Animals
MDPI AG., 11(2), 1-16.
https://doi.org/10.3390/ani11020398

Bajagai YS, Radovanović A, Steel JC, Stanley D. The effects of continual consumption of origanum vulgare on liver transcriptomics. in Animals. 2021;11(2):1-16.
doi:10.3390/ani11020398 .

Bajagai, Yadav S., Radovanović, Anita, Steel, Jason C., Stanley, Dragana, "The effects of continual consumption of origanum vulgare on liver transcriptomics" in Animals, 11, no. 2 (2021):1-16,
https://doi.org/10.3390/ani11020398 . .

TY  - JOUR
AU  - Gajdov, Vladimir
AU  - Radovanović, Anita
AU  - Lužajić Božinovski, Tijana
AU  - Marković, Danica
AU  - Stajković, Silvana
AU  - Milošević, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2389
AB  - The application of histological methods in meat composition analysis and identification of prohibited tissues and organs added to meat and meat products is still in the research phase, although there have been some promising results. The aim of this work was to assess the possibility of using histological and histochemical methods for analysis of kulen composition. In this research, six samples of kulen were examined, one of which was produced in domestic conditions, while the rest were commercial products sampled from local markets. The samples were carried through classical histological preparation. The obtained slides were stained with haematoxylin/eosin, Masson-Goldner, toluidine blue and periodic acid-Schiff/alcian blue. The content of muscle, fat and connective tissue was evaluated using histomorphometric analysis. Histological analysis of kulen composition determined the presence of the following structures: muscle, adipose and connective tissues, blood vessels, glandular epithelium, peripheral nerve, cartilage and plant tissue. The histomorphometric analysis showed that the kulen products contained on average 56±2.52% muscle tissue, 7.27±1.38% connective tissue and 19.82±3.24% adipose tissue. The results show that by applying histological methods it is possible to identify different permitted and prohibited animal tissues in kulen, and hence, it is possible to analyse the composition of kulen. It is also possible to confirm the presence of various plant tissues, but for their precise identification, additional histological methods are needed.
AB  - Primena histoloških metoda u analizi sastava mesa i utvrđivanju prisustva nedozvoljenih tkiva u mesu i proizvodima od mesa je još uvek u fazi istraživanja, iako su postojeći rezultati u ovoj oblasti obeć avajuć i. Cilj našeg istraživanja bio je ispitivanje moguć nosti korišć enja histoloških i histohemijskih metoda u analizi sastava fermentisane kobasice u tipu kulena. U ovom istraživanju ispitano je šest uzoraka kulena, od kojih je jedan proizveden u domać im uslovima, dok su ostali komercijalni proizvodi poticali iz lokalnih marketa. Uzorci su sprovedeni klasičnom histološkom procedurom. Dobijeni isečci bojeni su metodama: hematoksilin/ eosin,Masson-Goldner, toluidin plavo i perjodna kiselina/Schiff/alcijan plavo. Sadržaj mišić nog, masnog i vezivnog tkiva procenjen je primenom histomorfometrijskih analiza. Histološkom analizom sastava kulena utvrđeno je prisustvo sledeć ih struktura: mišić no, masno i vezivno tkivo, krvni sudovi, epitel žlezdanog tipa, periferni nerv, hrskavica i biljno tkivo. Histomorfometrijska analiza je pokazala da proizvodi sadrže u proseku 56±2,52% mišić nog tkiva, 7,27±1,38% vezivnog tkiva i 19,82±3,24% masnog tkiva. Rezultati pokazuju da je primenom histoloških metoda moguć e identifikovati različita dozvoljena i nedozvoljena životinjska tkiva u kulenu, pa je samim tim moguće analizirati sastav kulena. Takođe je moguć e potvrditi prisustvo različitih biljnih tkiva, ali je za njihovu preciznu identifikaciju neophodno primeniti dodatne metode.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Histological and histochemical analysis of dry fermented sausage of kulen composition
T1  - Histološka i histohemijska analiza sastava fermentisane kobasice u tipu kulena
VL  - 62
IS  - 2
SP  - 104
EP  - 112
DO  - 10.18485/MEATTECH.2021.62.2.3
ER  - 

@article{
author = "Gajdov, Vladimir and Radovanović, Anita and Lužajić Božinovski, Tijana and Marković, Danica and Stajković, Silvana and Milošević, Ivan",
year = "2021",
abstract = "The application of histological methods in meat composition analysis and identification of prohibited tissues and organs added to meat and meat products is still in the research phase, although there have been some promising results. The aim of this work was to assess the possibility of using histological and histochemical methods for analysis of kulen composition. In this research, six samples of kulen were examined, one of which was produced in domestic conditions, while the rest were commercial products sampled from local markets. The samples were carried through classical histological preparation. The obtained slides were stained with haematoxylin/eosin, Masson-Goldner, toluidine blue and periodic acid-Schiff/alcian blue. The content of muscle, fat and connective tissue was evaluated using histomorphometric analysis. Histological analysis of kulen composition determined the presence of the following structures: muscle, adipose and connective tissues, blood vessels, glandular epithelium, peripheral nerve, cartilage and plant tissue. The histomorphometric analysis showed that the kulen products contained on average 56±2.52% muscle tissue, 7.27±1.38% connective tissue and 19.82±3.24% adipose tissue. The results show that by applying histological methods it is possible to identify different permitted and prohibited animal tissues in kulen, and hence, it is possible to analyse the composition of kulen. It is also possible to confirm the presence of various plant tissues, but for their precise identification, additional histological methods are needed., Primena histoloških metoda u analizi sastava mesa i utvrđivanju prisustva nedozvoljenih tkiva u mesu i proizvodima od mesa je još uvek u fazi istraživanja, iako su postojeći rezultati u ovoj oblasti obeć avajuć i. Cilj našeg istraživanja bio je ispitivanje moguć nosti korišć enja histoloških i histohemijskih metoda u analizi sastava fermentisane kobasice u tipu kulena. U ovom istraživanju ispitano je šest uzoraka kulena, od kojih je jedan proizveden u domać im uslovima, dok su ostali komercijalni proizvodi poticali iz lokalnih marketa. Uzorci su sprovedeni klasičnom histološkom procedurom. Dobijeni isečci bojeni su metodama: hematoksilin/ eosin,Masson-Goldner, toluidin plavo i perjodna kiselina/Schiff/alcijan plavo. Sadržaj mišić nog, masnog i vezivnog tkiva procenjen je primenom histomorfometrijskih analiza. Histološkom analizom sastava kulena utvrđeno je prisustvo sledeć ih struktura: mišić no, masno i vezivno tkivo, krvni sudovi, epitel žlezdanog tipa, periferni nerv, hrskavica i biljno tkivo. Histomorfometrijska analiza je pokazala da proizvodi sadrže u proseku 56±2,52% mišić nog tkiva, 7,27±1,38% vezivnog tkiva i 19,82±3,24% masnog tkiva. Rezultati pokazuju da je primenom histoloških metoda moguć e identifikovati različita dozvoljena i nedozvoljena životinjska tkiva u kulenu, pa je samim tim moguće analizirati sastav kulena. Takođe je moguć e potvrditi prisustvo različitih biljnih tkiva, ali je za njihovu preciznu identifikaciju neophodno primeniti dodatne metode.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Histological and histochemical analysis of dry fermented sausage of kulen composition, Histološka i histohemijska analiza sastava fermentisane kobasice u tipu kulena",
volume = "62",
number = "2",
pages = "104-112",
doi = "10.18485/MEATTECH.2021.62.2.3"
}

Gajdov, V., Radovanović, A., Lužajić Božinovski, T., Marković, D., Stajković, S.,& Milošević, I.. (2021). Histological and histochemical analysis of dry fermented sausage of kulen composition. in Meat Technology
Institute of Meat Hygiene and Technology., 62(2), 104-112.
https://doi.org/10.18485/MEATTECH.2021.62.2.3

Gajdov V, Radovanović A, Lužajić Božinovski T, Marković D, Stajković S, Milošević I. Histological and histochemical analysis of dry fermented sausage of kulen composition. in Meat Technology. 2021;62(2):104-112.
doi:10.18485/MEATTECH.2021.62.2.3 .

Gajdov, Vladimir, Radovanović, Anita, Lužajić Božinovski, Tijana, Marković, Danica, Stajković, Silvana, Milošević, Ivan, "Histological and histochemical analysis of dry fermented sausage of kulen composition" in Meat Technology, 62, no. 2 (2021):104-112,
https://doi.org/10.18485/MEATTECH.2021.62.2.3 . .

TY  - CONF
AU  - Milošević, Ivan
AU  - Lužajić Božinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Gajdov, Vladimir
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2609
AB  - Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the
effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from 
subclinical and overt hypothyroid does, in their early postnatal development.Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was
administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male
pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess
the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was
performed by counting the number of positive dots in chondrocytes.The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte
cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal
hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation.We conclude that both forms of maternal hypothyroidism
in rats lead to accelerated proliferation, slowed autophagy and
compromised apoptosis of terminal hypertrophic chondrocytes in the
early infantile period and a delay in cartilage to bone transition.
C3  - 9th International Conference Veterinary Science and Profession 2021, Zagreb
T1  - Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups
SP  - 114
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2609
ER  - 

@conference{
author = "Milošević, Ivan and Lužajić Božinovski, Tijana and Danilović Luković, Jelena and Gajdov, Vladimir and Marković, Danica and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2021",
abstract = "Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the
effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from 
subclinical and overt hypothyroid does, in their early postnatal development.Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was
administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male
pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess
the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was
performed by counting the number of positive dots in chondrocytes.The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte
cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal
hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation.We conclude that both forms of maternal hypothyroidism
in rats lead to accelerated proliferation, slowed autophagy and
compromised apoptosis of terminal hypertrophic chondrocytes in the
early infantile period and a delay in cartilage to bone transition.",
journal = "9th International Conference Veterinary Science and Profession 2021, Zagreb",
title = "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups",
pages = "114-114",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2609"
}

Milošević, I., Lužajić Božinovski, T., Danilović Luković, J., Gajdov, V., Marković, D., Kovačević Filipović, M.,& Radovanović, A.. (2021). Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb, 114-114.
https://hdl.handle.net/21.15107/rcub_veterinar_2609

Milošević I, Lužajić Božinovski T, Danilović Luković J, Gajdov V, Marković D, Kovačević Filipović M, Radovanović A. Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb. 2021;:114-114.
https://hdl.handle.net/21.15107/rcub_veterinar_2609 .

Milošević, Ivan, Lužajić Božinovski, Tijana, Danilović Luković, Jelena, Gajdov, Vladimir, Marković, Danica, Kovačević Filipović, Milica, Radovanović, Anita, "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups" in 9th International Conference Veterinary Science and Profession 2021, Zagreb (2021):114-114,
https://hdl.handle.net/21.15107/rcub_veterinar_2609 .

TY  - CONF
AU  - Gajdov, Vladimir
AU  - Radovanović, Anita
AU  - Lužajić Božinovski, Tijana
AU  - Marković, Danica
AU  - Stajković, Silvana
AU  - Milošević, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2595
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 9th International Congress, Veterinary science and  profession, Zagreb
T1  - Application of histological and histochemical methods in kulen composition analysis
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2595
ER  - 

@conference{
author = "Gajdov, Vladimir and Radovanović, Anita and Lužajić Božinovski, Tijana and Marković, Danica and Stajković, Silvana and Milošević, Ivan",
year = "2021",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "9th International Congress, Veterinary science and  profession, Zagreb",
title = "Application of histological and histochemical methods in kulen composition analysis",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2595"
}

Gajdov, V., Radovanović, A., Lužajić Božinovski, T., Marković, D., Stajković, S.,& Milošević, I.. (2021). Application of histological and histochemical methods in kulen composition analysis. in 9th International Congress, Veterinary science and  profession, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 52-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2595

Gajdov V, Radovanović A, Lužajić Božinovski T, Marković D, Stajković S, Milošević I. Application of histological and histochemical methods in kulen composition analysis. in 9th International Congress, Veterinary science and  profession, Zagreb. 2021;:52-52.
https://hdl.handle.net/21.15107/rcub_veterinar_2595 .

Gajdov, Vladimir, Radovanović, Anita, Lužajić Božinovski, Tijana, Marković, Danica, Stajković, Silvana, Milošević, Ivan, "Application of histological and histochemical methods in kulen composition analysis" in 9th International Congress, Veterinary science and  profession, Zagreb (2021):52-52,
https://hdl.handle.net/21.15107/rcub_veterinar_2595 .

TY  - JOUR
AU  - Podpecan, Ozbalt
AU  - Zrimsek, Petra
AU  - Mrkun, Janko
AU  - Golicnik, Marko
AU  - Radovanović, Anita
AU  - Jovanović, Ljubomir
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1823
AB  - The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Italian Journal of Animal Science
T1  - Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows
VL  - 19
IS  - 1
SP  - 303
EP  - 309
DO  - 10.1080/1828051X.2020.1740064
ER  - 

@article{
author = "Podpecan, Ozbalt and Zrimsek, Petra and Mrkun, Janko and Golicnik, Marko and Radovanović, Anita and Jovanović, Ljubomir and Vujanac, Ivan and Prodanović, Radiša and Kirovski, Danijela",
year = "2020",
abstract = "The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Italian Journal of Animal Science",
title = "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows",
volume = "19",
number = "1",
pages = "303-309",
doi = "10.1080/1828051X.2020.1740064"
}

Podpecan, O., Zrimsek, P., Mrkun, J., Golicnik, M., Radovanović, A., Jovanović, L., Vujanac, I., Prodanović, R.,& Kirovski, D.. (2020). Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science
Taylor & Francis Ltd, Abingdon., 19(1), 303-309.
https://doi.org/10.1080/1828051X.2020.1740064

Podpecan O, Zrimsek P, Mrkun J, Golicnik M, Radovanović A, Jovanović L, Vujanac I, Prodanović R, Kirovski D. Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science. 2020;19(1):303-309.
doi:10.1080/1828051X.2020.1740064 .

Podpecan, Ozbalt, Zrimsek, Petra, Mrkun, Janko, Golicnik, Marko, Radovanović, Anita, Jovanović, Ljubomir, Vujanac, Ivan, Prodanović, Radiša, Kirovski, Danijela, "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows" in Italian Journal of Animal Science, 19, no. 1 (2020):303-309,
https://doi.org/10.1080/1828051X.2020.1740064 . .

TY  - JOUR
AU  - Glišić, Milica
AU  - Bošković, Marija
AU  - Baltić, Milan Ž.
AU  - Šefer, Dragan
AU  - Radovanović, Anita
AU  - Đorđevic, Vesna
AU  - Rašeta, Mladen
AU  - Marković, Radmila
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1969
AB  - The effect of dietary genistein on performance, intestinal morphology, caecal Lactobacillus spp. count, and tibia composition in broiler chickens after 21 and 37 days of feeding was investigated. A total of 360 Cobb 500 broiler chickens (21 days old) were randomly allocated to five treatments with six replicates of 12 birds each. They were fed a basal diet (C) or a basal diet supplemented with 200 (T1), 400 (T2), 600 (T3) and 800 (T4) mg genistein/kg of feed. Genistein supplementation did not affect feed intake, but improved bodyweight, weight gain and feed conversion ratio (FCR) after 21 days, while 600 mg/kg led to a significant increase in FCR after 37 days of feeding. Plasma triglyceride level decreased with dietary genistein after 21 days, while increases were found in T3 and T4 groups after prolonged supplementation. Significantly improved duodenal and jejunal villus length and width, crypt depth and villus/crypt ratio were observed after the first and the second finishing periods, respectively, while adverse effects were found in the ileum for both periods. At 42 days old, greater spleen and heart weights were measured in broilers fed diets with 800 mg/kg than in other broiler groups. The shorter genistein supplementation period (21 days) of 200 and 400 mg/kg had a positive effect on tibia wet weight, ash and calcium (Ca) content, while 37 days of the higher genistein doses administered to the T2, T3 and T4 birds significantly increased caecal lactic acid bacteria (LAB) counts. Thus, recommended doses should not exceed 400 mg/kg.
PB  - South African Bureau for Scientific Publications
T2  - South African Journal of Animal Science
T1  - Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein
VL  - 50
IS  - 2
SP  - 241
EP  - 252
DO  - 10.4314/SAJAS.V50I2.7
ER  - 

@article{
author = "Glišić, Milica and Bošković, Marija and Baltić, Milan Ž. and Šefer, Dragan and Radovanović, Anita and Đorđevic, Vesna and Rašeta, Mladen and Marković, Radmila",
year = "2020",
abstract = "The effect of dietary genistein on performance, intestinal morphology, caecal Lactobacillus spp. count, and tibia composition in broiler chickens after 21 and 37 days of feeding was investigated. A total of 360 Cobb 500 broiler chickens (21 days old) were randomly allocated to five treatments with six replicates of 12 birds each. They were fed a basal diet (C) or a basal diet supplemented with 200 (T1), 400 (T2), 600 (T3) and 800 (T4) mg genistein/kg of feed. Genistein supplementation did not affect feed intake, but improved bodyweight, weight gain and feed conversion ratio (FCR) after 21 days, while 600 mg/kg led to a significant increase in FCR after 37 days of feeding. Plasma triglyceride level decreased with dietary genistein after 21 days, while increases were found in T3 and T4 groups after prolonged supplementation. Significantly improved duodenal and jejunal villus length and width, crypt depth and villus/crypt ratio were observed after the first and the second finishing periods, respectively, while adverse effects were found in the ileum for both periods. At 42 days old, greater spleen and heart weights were measured in broilers fed diets with 800 mg/kg than in other broiler groups. The shorter genistein supplementation period (21 days) of 200 and 400 mg/kg had a positive effect on tibia wet weight, ash and calcium (Ca) content, while 37 days of the higher genistein doses administered to the T2, T3 and T4 birds significantly increased caecal lactic acid bacteria (LAB) counts. Thus, recommended doses should not exceed 400 mg/kg.",
publisher = "South African Bureau for Scientific Publications",
journal = "South African Journal of Animal Science",
title = "Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein",
volume = "50",
number = "2",
pages = "241-252",
doi = "10.4314/SAJAS.V50I2.7"
}

Glišić, M., Bošković, M., Baltić, M. Ž., Šefer, D., Radovanović, A., Đorđevic, V., Rašeta, M.,& Marković, R.. (2020). Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein. in South African Journal of Animal Science
South African Bureau for Scientific Publications., 50(2), 241-252.
https://doi.org/10.4314/SAJAS.V50I2.7

Glišić M, Bošković M, Baltić MŽ, Šefer D, Radovanović A, Đorđevic V, Rašeta M, Marković R. Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein. in South African Journal of Animal Science. 2020;50(2):241-252.
doi:10.4314/SAJAS.V50I2.7 .

Glišić, Milica, Bošković, Marija, Baltić, Milan Ž., Šefer, Dragan, Radovanović, Anita, Đorđevic, Vesna, Rašeta, Mladen, Marković, Radmila, "Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein" in South African Journal of Animal Science, 50, no. 2 (2020):241-252,
https://doi.org/10.4314/SAJAS.V50I2.7 . .

TY  - CONF
AU  - Marković, Lazar
AU  - Lazarević Macanović, Mirjana
AU  - Lužajić Božinovski, Tijana
AU  - Milovanović, Petar
AU  - Mitrović, Marko Jumake
AU  - Gajdov, Vladimir
AU  - Radovanović, Anita
AU  - Kovačević Filipović, Milica
AU  - Milošević, Ivan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2606
AB  - Radni konji se u Srbiji koriste kao tovarne životinje koje nose/vuku tovar, često teži od njihove telesne mase. Postavili smo hipotezu da opterećenje koje trpe utiče na pojavu lezija na koštano-zglobnom sistemu. Cilj ovog rada je bio da se uporedno prikaže prisustvo i stepen oštećenja zglobne hrskavice (ZH) i subhondralne kosti (SHK), distalnih kondilusa treće metatarzalne kosti kod starijeg radnog konja pomoću makroskopskog pregleda hrskavice, radioloških i histoloških metoda. Na lokalnoj klanici uzorkovani su kičični zglobovi ekstremiteta radnog pastuva, starog 10 godina. Uz pomoć kompjuterizovane tomografije (CT) utvrđene su promene samo na zglobu prednje leve noge. Nakon disekcije označenog zgloba, izvršena je biopsija ZH i SHK metakarpalne kosti u predelu oštećenja, a bioptati su snimljeni mikro CT-om nakon čega su deklacifikovani i ukalupljeni u parafin, i analizirani su preseci. Makroskopski je bilo vidljivo da je lokalizacija oštećenja ZH bila simetrična na medijalnim i lateralnim epikondilusima matakarpalnih i metatarzalnih kostiju svih ekstremiteta. Linije habanja i palmarne artroze bile su slabo do umereno izražene. Na nativnim rendgenogramima zglobova nisu uočene patološke promene. Analizom snimaka načinjenih CT-om uočena je promena u subhondralnoj zoni distalne epifize treće metakarpalne kosti u vidu pukotine, a oko nje je primetna zona skleroze SHK. Na mikro CT snimku uzorka koštanog tkiva uzetog u predelu lateralnog epikondilusa metakarpane kosti, takođe je uočljiva zona subhondralne skleroze. Najmarkatnije histološke promene na ZH su bile: na površini ZH undulacije i fisure, koje se pružaju i do duboke zone, mnogobrojne izogene grupe sa većim brojem hondrocita u lakuni, fokalni gubitak ćelija i mestimičnio prisustvo hipertrofičnih hondrocita. U SHK su primećene: neznatne lezije koštanog matriksa u vidu svetlijeg prebojavanja, blago remodeliranje kosti koje se karakteriše njenim napredovanjem ka kalcifikovanoj hrskavici. Dugotrajno opterećenje utiče na pojavu oštećenja SHK koje se može uočiti uz pomoć CT-a i histološkom analizom, dok se stepen oštećenja ZH može evaluirati makroskopski i histološki.
C3  - 25. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić
T1  - Analiza promena na hrskavici i subhondralnoj kosti kiĉiĉnog zgloba radnog konja - radiološki i histološki pristup
SP  - 138
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2606
ER  - 

@conference{
author = "Marković, Lazar and Lazarević Macanović, Mirjana and Lužajić Božinovski, Tijana and Milovanović, Petar and Mitrović, Marko Jumake and Gajdov, Vladimir and Radovanović, Anita and Kovačević Filipović, Milica and Milošević, Ivan",
year = "2020",
abstract = "Radni konji se u Srbiji koriste kao tovarne životinje koje nose/vuku tovar, često teži od njihove telesne mase. Postavili smo hipotezu da opterećenje koje trpe utiče na pojavu lezija na koštano-zglobnom sistemu. Cilj ovog rada je bio da se uporedno prikaže prisustvo i stepen oštećenja zglobne hrskavice (ZH) i subhondralne kosti (SHK), distalnih kondilusa treće metatarzalne kosti kod starijeg radnog konja pomoću makroskopskog pregleda hrskavice, radioloških i histoloških metoda. Na lokalnoj klanici uzorkovani su kičični zglobovi ekstremiteta radnog pastuva, starog 10 godina. Uz pomoć kompjuterizovane tomografije (CT) utvrđene su promene samo na zglobu prednje leve noge. Nakon disekcije označenog zgloba, izvršena je biopsija ZH i SHK metakarpalne kosti u predelu oštećenja, a bioptati su snimljeni mikro CT-om nakon čega su deklacifikovani i ukalupljeni u parafin, i analizirani su preseci. Makroskopski je bilo vidljivo da je lokalizacija oštećenja ZH bila simetrična na medijalnim i lateralnim epikondilusima matakarpalnih i metatarzalnih kostiju svih ekstremiteta. Linije habanja i palmarne artroze bile su slabo do umereno izražene. Na nativnim rendgenogramima zglobova nisu uočene patološke promene. Analizom snimaka načinjenih CT-om uočena je promena u subhondralnoj zoni distalne epifize treće metakarpalne kosti u vidu pukotine, a oko nje je primetna zona skleroze SHK. Na mikro CT snimku uzorka koštanog tkiva uzetog u predelu lateralnog epikondilusa metakarpane kosti, takođe je uočljiva zona subhondralne skleroze. Najmarkatnije histološke promene na ZH su bile: na površini ZH undulacije i fisure, koje se pružaju i do duboke zone, mnogobrojne izogene grupe sa većim brojem hondrocita u lakuni, fokalni gubitak ćelija i mestimičnio prisustvo hipertrofičnih hondrocita. U SHK su primećene: neznatne lezije koštanog matriksa u vidu svetlijeg prebojavanja, blago remodeliranje kosti koje se karakteriše njenim napredovanjem ka kalcifikovanoj hrskavici. Dugotrajno opterećenje utiče na pojavu oštećenja SHK koje se može uočiti uz pomoć CT-a i histološkom analizom, dok se stepen oštećenja ZH može evaluirati makroskopski i histološki.",
journal = "25. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić",
title = "Analiza promena na hrskavici i subhondralnoj kosti kiĉiĉnog zgloba radnog konja - radiološki i histološki pristup",
pages = "138-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2606"
}

Marković, L., Lazarević Macanović, M., Lužajić Božinovski, T., Milovanović, P., Mitrović, M. J., Gajdov, V., Radovanović, A., Kovačević Filipović, M.,& Milošević, I.. (2020). Analiza promena na hrskavici i subhondralnoj kosti kiĉiĉnog zgloba radnog konja - radiološki i histološki pristup. in 25. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić, 138-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2606

Marković L, Lazarević Macanović M, Lužajić Božinovski T, Milovanović P, Mitrović MJ, Gajdov V, Radovanović A, Kovačević Filipović M, Milošević I. Analiza promena na hrskavici i subhondralnoj kosti kiĉiĉnog zgloba radnog konja - radiološki i histološki pristup. in 25. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić. 2020;:138-141.
https://hdl.handle.net/21.15107/rcub_veterinar_2606 .

Marković, Lazar, Lazarević Macanović, Mirjana, Lužajić Božinovski, Tijana, Milovanović, Petar, Mitrović, Marko Jumake, Gajdov, Vladimir, Radovanović, Anita, Kovačević Filipović, Milica, Milošević, Ivan, "Analiza promena na hrskavici i subhondralnoj kosti kiĉiĉnog zgloba radnog konja - radiološki i histološki pristup" in 25. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Teslić (2020):138-141,
https://hdl.handle.net/21.15107/rcub_veterinar_2606 .

TY  - JOUR
AU  - Bauer, Benjamin W.
AU  - Radovanović, Anita
AU  - Willson, Nicky-Lee
AU  - Bajagai, Yadav Sharma
AU  - Thi, Thu Hao Van
AU  - Moore, Robert J.
AU  - Stanley, Dragana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1763
AB  - Prophylactic use of antibiotics in poultry diets has been identified as a problematic practice because of its potential to exacerbate the spread of antibiotic resistance to human pathogens. A range of countries have opted to completely ban the use of antibiotics in animal feed. The animal production industries are looking for alternative ways to effectively control pathogens while providing the performance benefits previously secured by antibiotics in feed. Here, we present evidence that oregano (Origanum vulgare) could be a potential alternative for pathogen control in the poultry industry. Broiler diets were supplemented with oregano powder (0%, 0.5%, 1%, and 2%) for six weeks. The capacity for pathogen control was estimated by microbiota profiling of the jejunum, ileum, and caecum content, and in the faeces, by 16S rRNA gene amplicon sequencing. The concentrations of short-chain fatty acids in the caecal content were also measured, as were villus/crypt parameters in the ileum. There were no differences among treatments in weight gain, feed intake, or the concentration of short-chain fatty acids. The height, width, and the surface area of villi in the ileum were not influenced by oregano addition. However, 1% and 2% of oregano produced a significant increase in the villus height to crypt depth ratio. There were no visible histopathological changes in the liver in control and treated groups. Although oregano had no significant effect on overall microbial diversity and gross composition, some specific genera, like Proteus, Klebsiella and Staphylococcus, which include known pathogens, were reduced in relative abundance by oregano treatment. Bifidobacterium, recognized as a beneficial and probiotic genus, was also suppressed by the oregano treatment.
PB  - Elsevier Sci Ltd, Oxford
T2  - Heliyon
T1  - Oregano: A potential prophylactic treatment for the intestinal microbiota
VL  - 5
IS  - 10
SP  - e02625
DO  - 10.1016/j.heliyon.2019.e02625
ER  - 

@article{
author = "Bauer, Benjamin W. and Radovanović, Anita and Willson, Nicky-Lee and Bajagai, Yadav Sharma and Thi, Thu Hao Van and Moore, Robert J. and Stanley, Dragana",
year = "2019",
abstract = "Prophylactic use of antibiotics in poultry diets has been identified as a problematic practice because of its potential to exacerbate the spread of antibiotic resistance to human pathogens. A range of countries have opted to completely ban the use of antibiotics in animal feed. The animal production industries are looking for alternative ways to effectively control pathogens while providing the performance benefits previously secured by antibiotics in feed. Here, we present evidence that oregano (Origanum vulgare) could be a potential alternative for pathogen control in the poultry industry. Broiler diets were supplemented with oregano powder (0%, 0.5%, 1%, and 2%) for six weeks. The capacity for pathogen control was estimated by microbiota profiling of the jejunum, ileum, and caecum content, and in the faeces, by 16S rRNA gene amplicon sequencing. The concentrations of short-chain fatty acids in the caecal content were also measured, as were villus/crypt parameters in the ileum. There were no differences among treatments in weight gain, feed intake, or the concentration of short-chain fatty acids. The height, width, and the surface area of villi in the ileum were not influenced by oregano addition. However, 1% and 2% of oregano produced a significant increase in the villus height to crypt depth ratio. There were no visible histopathological changes in the liver in control and treated groups. Although oregano had no significant effect on overall microbial diversity and gross composition, some specific genera, like Proteus, Klebsiella and Staphylococcus, which include known pathogens, were reduced in relative abundance by oregano treatment. Bifidobacterium, recognized as a beneficial and probiotic genus, was also suppressed by the oregano treatment.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Heliyon",
title = "Oregano: A potential prophylactic treatment for the intestinal microbiota",
volume = "5",
number = "10",
pages = "e02625",
doi = "10.1016/j.heliyon.2019.e02625"
}

Bauer, B. W., Radovanović, A., Willson, N., Bajagai, Y. S., Thi, T. H. V., Moore, R. J.,& Stanley, D.. (2019). Oregano: A potential prophylactic treatment for the intestinal microbiota. in Heliyon
Elsevier Sci Ltd, Oxford., 5(10), e02625.
https://doi.org/10.1016/j.heliyon.2019.e02625

Bauer BW, Radovanović A, Willson N, Bajagai YS, Thi THV, Moore RJ, Stanley D. Oregano: A potential prophylactic treatment for the intestinal microbiota. in Heliyon. 2019;5(10):e02625.
doi:10.1016/j.heliyon.2019.e02625 .

Bauer, Benjamin W., Radovanović, Anita, Willson, Nicky-Lee, Bajagai, Yadav Sharma, Thi, Thu Hao Van, Moore, Robert J., Stanley, Dragana, "Oregano: A potential prophylactic treatment for the intestinal microbiota" in Heliyon, 5, no. 10 (2019):e02625,
https://doi.org/10.1016/j.heliyon.2019.e02625 . .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Radovanović, Anita
AU  - Milošević, Ivan
AU  - Lužajić Božinovski, Tijana
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
AU  - Korać, Aleksandra
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2597
AB  - The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.
PB  - Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology
PB  - Belgrade : Ministry of Education, Science and Technological Development
PB  - Belgrade : University of Belgrade
PB  - Belgrade : Faculty of Biology of University of Belgrade
C3  - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
T1  - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
SP  - 103
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2597
ER  - 

@conference{
author = "Danilović Luković, Jelena and Radovanović, Anita and Milošević, Ivan and Lužajić Božinovski, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra",
year = "2018",
abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.",
publisher = "Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology, Belgrade : Ministry of Education, Science and Technological Development, Belgrade : University of Belgrade, Belgrade : Faculty of Biology of University of Belgrade",
journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade",
title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection",
pages = "103-103",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2597"
}

Danilović Luković, J., Radovanović, A., Milošević, I., Lužajić Božinovski, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology., 103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597

Danilović Luković J, Radovanović A, Milošević I, Lužajić Božinovski T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

Danilović Luković, Jelena, Radovanović, Anita, Milošević, Ivan, Lužajić Božinovski, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):103-103,
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

TY  - JOUR
AU  - Baltić, Branislav
AU  - Ćirić, Jelena
AU  - Šefer, Dragan
AU  - Radovanović, Anita
AU  - Đorđević, Jasna
AU  - Glišić, Milica
AU  - Bošković, Marija
AU  - Baltić, Milan Ž.
AU  - Đorđević, V.
AU  - Marković, Radmila
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1578
AB  - The aim of the present study was to assess the effects of medium-chain fatty acid (MCFA) diet supplementation on growth performance, intestinal histomorphology, serum biochemistry and intestinal microflora of broiler chickens. The study was performed on 180 one-day-old broilers of the same origin (Cobb 500 hybrid), over a 42-day period. They were fed diets supplemented with three treatments: control group (basal diet without supplementation); group with MCFA supplementation; and group with MCFA and coccidiostat supplementation. Broiler chickens fed diets supplemented with MCFAs had a significantly greater final bodyweight. The weights of carcass cuts (breast, drumsticks with thighs and wings) were greater in broilers receiving MCFAs than in control broilers. The addition of MCFAs to broiler diet significantly increased villus length and crypt depth in the duodenum and caecum, and significantly decreased villus width in the duodenum and ileum. Additionally, serum HDL-cholesterol and triglyceride concentrations were increased significantly in broilers with MCFA dietary supplementation. The results indicated that the MCFA diet supplementation had a beneficial effect on the performance of broiler chickens, their intestinal histomorphology and microflora.
PB  - South African Journal Of Animal Sciences, Hatfield
T2  - South African Journal of Animal Science
T1  - Effect of dietary supplementation with medium chain fatty acids on growth performance, intestinal histomorphology, lipid profile and intestinal microflora of broiler chickens
VL  - 48
IS  - 5
SP  - 885
EP  - 896
DO  - 10.4314/sajas.v48i5.8
ER  - 

@article{
author = "Baltić, Branislav and Ćirić, Jelena and Šefer, Dragan and Radovanović, Anita and Đorđević, Jasna and Glišić, Milica and Bošković, Marija and Baltić, Milan Ž. and Đorđević, V. and Marković, Radmila",
year = "2018",
abstract = "The aim of the present study was to assess the effects of medium-chain fatty acid (MCFA) diet supplementation on growth performance, intestinal histomorphology, serum biochemistry and intestinal microflora of broiler chickens. The study was performed on 180 one-day-old broilers of the same origin (Cobb 500 hybrid), over a 42-day period. They were fed diets supplemented with three treatments: control group (basal diet without supplementation); group with MCFA supplementation; and group with MCFA and coccidiostat supplementation. Broiler chickens fed diets supplemented with MCFAs had a significantly greater final bodyweight. The weights of carcass cuts (breast, drumsticks with thighs and wings) were greater in broilers receiving MCFAs than in control broilers. The addition of MCFAs to broiler diet significantly increased villus length and crypt depth in the duodenum and caecum, and significantly decreased villus width in the duodenum and ileum. Additionally, serum HDL-cholesterol and triglyceride concentrations were increased significantly in broilers with MCFA dietary supplementation. The results indicated that the MCFA diet supplementation had a beneficial effect on the performance of broiler chickens, their intestinal histomorphology and microflora.",
publisher = "South African Journal Of Animal Sciences, Hatfield",
journal = "South African Journal of Animal Science",
title = "Effect of dietary supplementation with medium chain fatty acids on growth performance, intestinal histomorphology, lipid profile and intestinal microflora of broiler chickens",
volume = "48",
number = "5",
pages = "885-896",
doi = "10.4314/sajas.v48i5.8"
}

Baltić, B., Ćirić, J., Šefer, D., Radovanović, A., Đorđević, J., Glišić, M., Bošković, M., Baltić, M. Ž., Đorđević, V.,& Marković, R.. (2018). Effect of dietary supplementation with medium chain fatty acids on growth performance, intestinal histomorphology, lipid profile and intestinal microflora of broiler chickens. in South African Journal of Animal Science
South African Journal Of Animal Sciences, Hatfield., 48(5), 885-896.
https://doi.org/10.4314/sajas.v48i5.8

Baltić B, Ćirić J, Šefer D, Radovanović A, Đorđević J, Glišić M, Bošković M, Baltić MŽ, Đorđević V, Marković R. Effect of dietary supplementation with medium chain fatty acids on growth performance, intestinal histomorphology, lipid profile and intestinal microflora of broiler chickens. in South African Journal of Animal Science. 2018;48(5):885-896.
doi:10.4314/sajas.v48i5.8 .

Baltić, Branislav, Ćirić, Jelena, Šefer, Dragan, Radovanović, Anita, Đorđević, Jasna, Glišić, Milica, Bošković, Marija, Baltić, Milan Ž., Đorđević, V., Marković, Radmila, "Effect of dietary supplementation with medium chain fatty acids on growth performance, intestinal histomorphology, lipid profile and intestinal microflora of broiler chickens" in South African Journal of Animal Science, 48, no. 5 (2018):885-896,
https://doi.org/10.4314/sajas.v48i5.8 . .

TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanović, Anita
AU  - Danilović-Luković, Jelena
AU  - Lužajić Božinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Vejnović, Branislav
AU  - Kovačević-Filipović, Milica
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1588
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
VL  - 68
IS  - 3
SP  - 301
EP  - 320
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanović, Anita and Danilović-Luković, Jelena and Lužajić Božinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Vejnović, Branislav and Kovačević-Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
volume = "68",
number = "3",
pages = "301-320",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanović, A., Danilović-Luković, J., Lužajić Božinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J., Vejnović, B.,& Kovačević-Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanović A, Danilović-Luković J, Lužajić Božinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Vejnović B, Kovačević-Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanović, Anita, Danilović-Luković, Jelena, Lužajić Božinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Vejnović, Branislav, Kovačević-Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .

TY  - JOUR
AU  - Dedać-Delkić, Ana
AU  - Milovanović, Zorana
AU  - Božović-Ilić, Anja
AU  - Radovanović, Anita
AU  - Kovačević-Filipović, Milica
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1604
AB  - Introduction. Analysis of canine lipoprotein fractions after agarose gel electrophoresis (agEF) separation could be an important diagnostic tool in primary and secondary dyslipidemia diagnosis. The aim of this study was to measure concentrations of triglycerides and cholesterol and to analyze lipoprotein fractions in dogs after basal (12 hours) and short-term (24 and 36 hours) fasting, i.e., frequent conditions in clinical practice. Materials and Methods. Blood samples were collected from six lean dogs of both sexes and different breeds, after 12, 24 and 36 hours of fasting. Concentrations of glucose, triglycerides and cholesterol were determined on an automated wet biochemistry analyzer, lipoprotein fractions were separated by agEF and leukocyte numbers were assessed on an automated hematology analyzer. Results and Conclusions. Results showed there was no significant change in glucose, triglyceride and cholesterol concentrations nor in leukocyte numbers during dog fasting. Moreover, there was no change in α1- and α2-fractions, but there was a significant decrease in preβ- and β-fraction of lipoproteins. It is know that high density lipoproteins (HDL) have α-mobility and very low density (VLDL) and low density lipoproteins (LDL) have preβ- and β-mobility. Thus, it is possible that reverse cholesterol transport maintained by HDL is not affected during short-term fasting. On the contrary, synthesis of VLDL and formation of LDL are probably decreased because endogenous synthesis of triglycerides is decreased or their clearance is increased.
AB  - Uvod. Poznato je iz literature da se odnosi lipoproteinskih frakcija seruma pasa koje se dobijaju posle elektroforeze na agaroznom gelu (EFag) menjaju kod oboljenja praćenih dislipidemijama, što može biti jedan od značajnih elemenata dijagnostike pojedinih patoloških stanja. Stoga je cilj ovih ispitivanja bio da se utvrdi koncentracija triglicerida, holesterola i glukoze u serumu pasa kao i odnos lipoproteinskih frakcija dobijenih pomoću EFag, u uslovima koji se često sreću u kliničkoj praksi: posle bazalnog (12 časova) i kratkotrajnog gladovanja od 24 i 36 časova. Materijal i metode. Uzorci krvi su sakupljeni od šest pasa različitih rasa, normalne telesne kondicije, oba pola, posle 12, 24 i 36 časova gladovanja. Koncentracija glukoze, triglicerida i holesterola je određena na automatskom biohemijskom analizatoru. Lipoproteinske frakcije su razdvojene korišćenjem EFag. Broj leukocita je utvrđen na hematološkom analizatoru. Rezultati i zaključak. Rezultati ovog ispitivanja su pokazali da se tokom kratkotrajnog gladovanja kod pasa koncentracija glukoze, triglicerida i holesterola kao i broj leukocita ne menjaju. Osim toga, nije bilo promena vezanih za α1-i α2-elektroforetske zone, ali se javio značajan pad koncentracije lipoproteina preβ-i β-zone. Poznato je da se lipoproteini velike gustine (HDL) nalaze u α-zoni, dok lipoproteini veoma male gustine (VLDL) i lipoproteini male gustine (LDL) pripadaju preβ-i β-zoni. Na osnovu naših nalaza moguće je zaključiti da reverzni transport holesterola koji se odvija posredstvom HDL-a nije poremećen tokom kratkotrajnog gladovanja. Nasuprot tome, kratkotrajno gladovanje verovatno utiče na smanjen obim sinteze triglicerida u jetri, odnosno na smanjenu sintezu VLDL-a i LDL-a ili na njihovo efikasnije uklanjanje iz plazme.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Effects of short-term fasting on lipid and lipoprotein concentrations in healthy lean dogs
T1  - Efekat kratkotrajnog gladovanja na koncentraciju lipida i lipoproteina kod zdravih pasa normalne telesne kondicije
VL  - 72
IS  - 1
SP  - 35
EP  - 43
DO  - 10.2298/VETGL170917001D
ER  - 

@article{
author = "Dedać-Delkić, Ana and Milovanović, Zorana and Božović-Ilić, Anja and Radovanović, Anita and Kovačević-Filipović, Milica",
year = "2018",
abstract = "Introduction. Analysis of canine lipoprotein fractions after agarose gel electrophoresis (agEF) separation could be an important diagnostic tool in primary and secondary dyslipidemia diagnosis. The aim of this study was to measure concentrations of triglycerides and cholesterol and to analyze lipoprotein fractions in dogs after basal (12 hours) and short-term (24 and 36 hours) fasting, i.e., frequent conditions in clinical practice. Materials and Methods. Blood samples were collected from six lean dogs of both sexes and different breeds, after 12, 24 and 36 hours of fasting. Concentrations of glucose, triglycerides and cholesterol were determined on an automated wet biochemistry analyzer, lipoprotein fractions were separated by agEF and leukocyte numbers were assessed on an automated hematology analyzer. Results and Conclusions. Results showed there was no significant change in glucose, triglyceride and cholesterol concentrations nor in leukocyte numbers during dog fasting. Moreover, there was no change in α1- and α2-fractions, but there was a significant decrease in preβ- and β-fraction of lipoproteins. It is know that high density lipoproteins (HDL) have α-mobility and very low density (VLDL) and low density lipoproteins (LDL) have preβ- and β-mobility. Thus, it is possible that reverse cholesterol transport maintained by HDL is not affected during short-term fasting. On the contrary, synthesis of VLDL and formation of LDL are probably decreased because endogenous synthesis of triglycerides is decreased or their clearance is increased., Uvod. Poznato je iz literature da se odnosi lipoproteinskih frakcija seruma pasa koje se dobijaju posle elektroforeze na agaroznom gelu (EFag) menjaju kod oboljenja praćenih dislipidemijama, što može biti jedan od značajnih elemenata dijagnostike pojedinih patoloških stanja. Stoga je cilj ovih ispitivanja bio da se utvrdi koncentracija triglicerida, holesterola i glukoze u serumu pasa kao i odnos lipoproteinskih frakcija dobijenih pomoću EFag, u uslovima koji se često sreću u kliničkoj praksi: posle bazalnog (12 časova) i kratkotrajnog gladovanja od 24 i 36 časova. Materijal i metode. Uzorci krvi su sakupljeni od šest pasa različitih rasa, normalne telesne kondicije, oba pola, posle 12, 24 i 36 časova gladovanja. Koncentracija glukoze, triglicerida i holesterola je određena na automatskom biohemijskom analizatoru. Lipoproteinske frakcije su razdvojene korišćenjem EFag. Broj leukocita je utvrđen na hematološkom analizatoru. Rezultati i zaključak. Rezultati ovog ispitivanja su pokazali da se tokom kratkotrajnog gladovanja kod pasa koncentracija glukoze, triglicerida i holesterola kao i broj leukocita ne menjaju. Osim toga, nije bilo promena vezanih za α1-i α2-elektroforetske zone, ali se javio značajan pad koncentracije lipoproteina preβ-i β-zone. Poznato je da se lipoproteini velike gustine (HDL) nalaze u α-zoni, dok lipoproteini veoma male gustine (VLDL) i lipoproteini male gustine (LDL) pripadaju preβ-i β-zoni. Na osnovu naših nalaza moguće je zaključiti da reverzni transport holesterola koji se odvija posredstvom HDL-a nije poremećen tokom kratkotrajnog gladovanja. Nasuprot tome, kratkotrajno gladovanje verovatno utiče na smanjen obim sinteze triglicerida u jetri, odnosno na smanjenu sintezu VLDL-a i LDL-a ili na njihovo efikasnije uklanjanje iz plazme.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Effects of short-term fasting on lipid and lipoprotein concentrations in healthy lean dogs, Efekat kratkotrajnog gladovanja na koncentraciju lipida i lipoproteina kod zdravih pasa normalne telesne kondicije",
volume = "72",
number = "1",
pages = "35-43",
doi = "10.2298/VETGL170917001D"
}

Dedać-Delkić, A., Milovanović, Z., Božović-Ilić, A., Radovanović, A.,& Kovačević-Filipović, M.. (2018). Effects of short-term fasting on lipid and lipoprotein concentrations in healthy lean dogs. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(1), 35-43.
https://doi.org/10.2298/VETGL170917001D

Dedać-Delkić A, Milovanović Z, Božović-Ilić A, Radovanović A, Kovačević-Filipović M. Effects of short-term fasting on lipid and lipoprotein concentrations in healthy lean dogs. in Veterinarski Glasnik. 2018;72(1):35-43.
doi:10.2298/VETGL170917001D .

Dedać-Delkić, Ana, Milovanović, Zorana, Božović-Ilić, Anja, Radovanović, Anita, Kovačević-Filipović, Milica, "Effects of short-term fasting on lipid and lipoprotein concentrations in healthy lean dogs" in Veterinarski Glasnik, 72, no. 1 (2018):35-43,
https://doi.org/10.2298/VETGL170917001D . .

TY  - JOUR
AU  - Radovanović, Anita
AU  - Kovačević-Filipović, Milica
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Veličković, Stefan
AU  - Andrejević, Jana
AU  - Danilović-Luković, Jelena
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1607
AB  - Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodelling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period on morphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer.
AB  - Uvod. Tokom reproduktivnog perioda, nakon svake ovulacije, površinski epitel (PE) jajnika se intenzivno regeneriše i remodelira. Ovaj proces povećava rizik od nastajanja jednog od najčešćih tumora jajnika kod žena i kuja. Imajući u vidu činjenicu da hipotireoidizam majki u velikoj meri utiče na proliferaciju i ćelijsku smrt u toku folikulogeneze u ranom postnatalnom periodu, cilj rada je bio da analiziramo efekat hipotireoidizma majki na morfologiju i dinamiku PE jajnika potomaka. Materijal i metode. U eksperimentu su korišćeni tek rođeni (24 časa stari) i neonatalni (4 dana stari) mladunci koji potiču od kontrolnih i hipotireoidnih majki. Morfometrijskom analizom određivana je prosečna visina ćelija PE, izmerena na četiri mesta na svakom ispitivanom preseku jajnika. Takođe, imunopozitivnost proliferativnivnog ćelijskog nuklearnog antigena (PCNA) i kaspaze 3 je određivana semikvantitativno. Rezultati i zaključci. Veće promene u strukturi PE jajnika nisu zapažene između grupa, osim blagog povećanja visine ćelija i nepotpune apikalne membrane sa projekcijama citoplazme kod hipotireoidnih životinja. Imunopozitivnost PCNA je značajno povećana u jajnicima hipotireoidnih životinja obe starosne grupe u odnosu na kontrole, dok je imunopozitivnost na kaspazu 3 slabo izražena kod svih životinja. Naše istraživanje je pokazalo da hipotireoidizam u ranom postnatalnom periodu dovodi do promena u morfologiji i proliferaciji ćelija PE, bez efekta na uklanjanje ćelija delovanjem kaspaze 3. Ovaj model može da posluži u budućim istraživanjima potencijalne karcinogeneze u cilju prevencije i tretmana kancera jajnika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective
T1  - Površinski epitel jajnika hipotireoidnih novorođenih i neonatalnih pacova - iz perspektive PCNA i kaspaze-3
VL  - 72
IS  - 2
SP  - 80
EP  - 89
DO  - 10.2298/VETGL180320007R
ER  - 

@article{
author = "Radovanović, Anita and Kovačević-Filipović, Milica and Milošević, Ivan and Lužajić, Tijana and Veličković, Stefan and Andrejević, Jana and Danilović-Luković, Jelena",
year = "2018",
abstract = "Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodelling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period on morphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer., Uvod. Tokom reproduktivnog perioda, nakon svake ovulacije, površinski epitel (PE) jajnika se intenzivno regeneriše i remodelira. Ovaj proces povećava rizik od nastajanja jednog od najčešćih tumora jajnika kod žena i kuja. Imajući u vidu činjenicu da hipotireoidizam majki u velikoj meri utiče na proliferaciju i ćelijsku smrt u toku folikulogeneze u ranom postnatalnom periodu, cilj rada je bio da analiziramo efekat hipotireoidizma majki na morfologiju i dinamiku PE jajnika potomaka. Materijal i metode. U eksperimentu su korišćeni tek rođeni (24 časa stari) i neonatalni (4 dana stari) mladunci koji potiču od kontrolnih i hipotireoidnih majki. Morfometrijskom analizom određivana je prosečna visina ćelija PE, izmerena na četiri mesta na svakom ispitivanom preseku jajnika. Takođe, imunopozitivnost proliferativnivnog ćelijskog nuklearnog antigena (PCNA) i kaspaze 3 je određivana semikvantitativno. Rezultati i zaključci. Veće promene u strukturi PE jajnika nisu zapažene između grupa, osim blagog povećanja visine ćelija i nepotpune apikalne membrane sa projekcijama citoplazme kod hipotireoidnih životinja. Imunopozitivnost PCNA je značajno povećana u jajnicima hipotireoidnih životinja obe starosne grupe u odnosu na kontrole, dok je imunopozitivnost na kaspazu 3 slabo izražena kod svih životinja. Naše istraživanje je pokazalo da hipotireoidizam u ranom postnatalnom periodu dovodi do promena u morfologiji i proliferaciji ćelija PE, bez efekta na uklanjanje ćelija delovanjem kaspaze 3. Ovaj model može da posluži u budućim istraživanjima potencijalne karcinogeneze u cilju prevencije i tretmana kancera jajnika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective, Površinski epitel jajnika hipotireoidnih novorođenih i neonatalnih pacova - iz perspektive PCNA i kaspaze-3",
volume = "72",
number = "2",
pages = "80-89",
doi = "10.2298/VETGL180320007R"
}

Radovanović, A., Kovačević-Filipović, M., Milošević, I., Lužajić, T., Veličković, S., Andrejević, J.,& Danilović-Luković, J.. (2018). Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 80-89.
https://doi.org/10.2298/VETGL180320007R

Radovanović A, Kovačević-Filipović M, Milošević I, Lužajić T, Veličković S, Andrejević J, Danilović-Luković J. Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik. 2018;72(2):80-89.
doi:10.2298/VETGL180320007R .

Radovanović, Anita, Kovačević-Filipović, Milica, Milošević, Ivan, Lužajić, Tijana, Veličković, Stefan, Andrejević, Jana, Danilović-Luković, Jelena, "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective" in Veterinarski Glasnik, 72, no. 2 (2018):80-89,
https://doi.org/10.2298/VETGL180320007R . .

TY  - JOUR
AU  - Janjić, Jelena
AU  - Ivanović-Ćirić, Jelena
AU  - Aleksić, Jelena
AU  - Glamočlija, Nataša
AU  - Starcević, Marija
AU  - Radovanović, Anita
AU  - Baltić, Milan Ž.
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1462
AB  - The aim of this study was to evaluate the effects of immunocastration on pork meat and carcass quality, compared to meat from surgically castrated males and entire males. Ninety (Duroc x Pietrain) x (Landrace x Yorkshire) crossbred pigs were assigned to three experimental groups: surgically castrated males (barrows; castrated up to the seventh day of age), entire males (males), and vaccinated males (immunocastrates). Carcass and meat quality characteristics such as weight of hot and chilled carcass, meat yield, chilling loss and chemical parameters were examined. Surgically castrated pigs had significantly lower (p lt 0.01; p lt 0.05) weight before slaughter, than males and immunocastrates, and also lower (p lt 0.01) warm carcass weight than males. The average carcass meatiness of castrates was significantly lower (p lt 0.01) than the average meatiness of males and immunocastrates. Chilling loss of barrows was significantly lower (p lt 0.01) than chilling loss of males or immunocastrates. It was also found that the chilling loss of immunocastrates was significantly lower (p lt 0.05) than chilling loss of males. According to the results obtained, it can be concluded that immunocastration could be a good alternative to surgical castration considering meat and carcass quality characteristics.
PB  - Institute of meat hygiene and technology, Belgrade
T2  - Meat Technology
T1  - The effects of immunocastration on male pig yield parameters and meat quality
VL  - 58
IS  - 1
SP  - 1
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1462
ER  - 

@article{
author = "Janjić, Jelena and Ivanović-Ćirić, Jelena and Aleksić, Jelena and Glamočlija, Nataša and Starcević, Marija and Radovanović, Anita and Baltić, Milan Ž.",
year = "2017",
abstract = "The aim of this study was to evaluate the effects of immunocastration on pork meat and carcass quality, compared to meat from surgically castrated males and entire males. Ninety (Duroc x Pietrain) x (Landrace x Yorkshire) crossbred pigs were assigned to three experimental groups: surgically castrated males (barrows; castrated up to the seventh day of age), entire males (males), and vaccinated males (immunocastrates). Carcass and meat quality characteristics such as weight of hot and chilled carcass, meat yield, chilling loss and chemical parameters were examined. Surgically castrated pigs had significantly lower (p lt 0.01; p lt 0.05) weight before slaughter, than males and immunocastrates, and also lower (p lt 0.01) warm carcass weight than males. The average carcass meatiness of castrates was significantly lower (p lt 0.01) than the average meatiness of males and immunocastrates. Chilling loss of barrows was significantly lower (p lt 0.01) than chilling loss of males or immunocastrates. It was also found that the chilling loss of immunocastrates was significantly lower (p lt 0.05) than chilling loss of males. According to the results obtained, it can be concluded that immunocastration could be a good alternative to surgical castration considering meat and carcass quality characteristics.",
publisher = "Institute of meat hygiene and technology, Belgrade",
journal = "Meat Technology",
title = "The effects of immunocastration on male pig yield parameters and meat quality",
volume = "58",
number = "1",
pages = "1-9",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1462"
}

Janjić, J., Ivanović-Ćirić, J., Aleksić, J., Glamočlija, N., Starcević, M., Radovanović, A.,& Baltić, M. Ž.. (2017). The effects of immunocastration on male pig yield parameters and meat quality. in Meat Technology
Institute of meat hygiene and technology, Belgrade., 58(1), 1-9.
https://hdl.handle.net/21.15107/rcub_veterinar_1462

Janjić J, Ivanović-Ćirić J, Aleksić J, Glamočlija N, Starcević M, Radovanović A, Baltić MŽ. The effects of immunocastration on male pig yield parameters and meat quality. in Meat Technology. 2017;58(1):1-9.
https://hdl.handle.net/21.15107/rcub_veterinar_1462 .

Janjić, Jelena, Ivanović-Ćirić, Jelena, Aleksić, Jelena, Glamočlija, Nataša, Starcević, Marija, Radovanović, Anita, Baltić, Milan Ž., "The effects of immunocastration on male pig yield parameters and meat quality" in Meat Technology, 58, no. 1 (2017):1-9,
https://hdl.handle.net/21.15107/rcub_veterinar_1462 .

TY  - JOUR
AU  - Danilović-Luković, Jelena
AU  - Korac, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Milanović, Zorana
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1477
AB  - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
VL  - 67
IS  - 1
SP  - 92
EP  - 106
DO  - 10.1515/acve-2017-0009
ER  - 

@article{
author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Milanović, Zorana and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2017",
abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats",
volume = "67",
number = "1",
pages = "92-106",
doi = "10.1515/acve-2017-0009"
}

Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Milanović, Z., Kovačević-Filipović, M.,& Radovanović, A.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 92-106.
https://doi.org/10.1515/acve-2017-0009

Danilović-Luković J, Korac A, Milošević I, Lužajić T, Milanović Z, Kovačević-Filipović M, Radovanović A. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106.
doi:10.1515/acve-2017-0009 .

Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Milanović, Zorana, Kovačević-Filipović, Milica, Radovanović, Anita, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106,
https://doi.org/10.1515/acve-2017-0009 . .

TY  - JOUR
AU  - Milanović, Valentina
AU  - Radovanović, Anita
AU  - Vasilijić, Sasa
AU  - Mrvić, Verica
AU  - Milosević, Božidar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1494
AB  - Rabbit is the third most commonly used animal model in different fields of scientific research, such as reproductive biology, fertility and embryo transfer studies, and immunology. This animal species, often used in antibodies production, has minority of scientific records about the immunological status of its reproductive organs. The aim of this study was to find histological and immunological changes in rabbit female reproductive tract during different reproductive stages. The study was carried out on female rabbits, divided in three groups, according to the following stages of reproductive cycle: Estrous, ovulation and pregnancy. Histological and immunohistochemical stains for T- and B-cells were performed on tissue samples of cornu uteri and cervix. T lymphocytes were predominant in all anatomical parts of the uterus, in all stages of the cycle. The highest number of those cells was recorded at estrous, while the lowest was recorded at pregnancy. Cervix expressed more immunological activity than cornu uteri. The distribution and the number of immune positive cells in the rabbit female reproductive tract depend on its hormonal status.
PB  - Kafkas Univ, Veteriner Fakultesi Dergisi, Kars
T2  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
T1  - Histological and Immunological Changes in Uterus During the Different Reproductive Stages at Californian Rabbit (Oryctolagus cuniculus)
VL  - 23
IS  - 1
SP  - 137
EP  - 144
DO  - 10.9775/kvfd.2016.16008
ER  - 

@article{
author = "Milanović, Valentina and Radovanović, Anita and Vasilijić, Sasa and Mrvić, Verica and Milosević, Božidar",
year = "2017",
abstract = "Rabbit is the third most commonly used animal model in different fields of scientific research, such as reproductive biology, fertility and embryo transfer studies, and immunology. This animal species, often used in antibodies production, has minority of scientific records about the immunological status of its reproductive organs. The aim of this study was to find histological and immunological changes in rabbit female reproductive tract during different reproductive stages. The study was carried out on female rabbits, divided in three groups, according to the following stages of reproductive cycle: Estrous, ovulation and pregnancy. Histological and immunohistochemical stains for T- and B-cells were performed on tissue samples of cornu uteri and cervix. T lymphocytes were predominant in all anatomical parts of the uterus, in all stages of the cycle. The highest number of those cells was recorded at estrous, while the lowest was recorded at pregnancy. Cervix expressed more immunological activity than cornu uteri. The distribution and the number of immune positive cells in the rabbit female reproductive tract depend on its hormonal status.",
publisher = "Kafkas Univ, Veteriner Fakultesi Dergisi, Kars",
journal = "Kafkas Üniversitesi Veteriner Fakültesi Dergisi",
title = "Histological and Immunological Changes in Uterus During the Different Reproductive Stages at Californian Rabbit (Oryctolagus cuniculus)",
volume = "23",
number = "1",
pages = "137-144",
doi = "10.9775/kvfd.2016.16008"
}

Milanović, V., Radovanović, A., Vasilijić, S., Mrvić, V.,& Milosević, B.. (2017). Histological and Immunological Changes in Uterus During the Different Reproductive Stages at Californian Rabbit (Oryctolagus cuniculus). in Kafkas Üniversitesi Veteriner Fakültesi Dergisi
Kafkas Univ, Veteriner Fakultesi Dergisi, Kars., 23(1), 137-144.
https://doi.org/10.9775/kvfd.2016.16008

Milanović V, Radovanović A, Vasilijić S, Mrvić V, Milosević B. Histological and Immunological Changes in Uterus During the Different Reproductive Stages at Californian Rabbit (Oryctolagus cuniculus). in Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017;23(1):137-144.
doi:10.9775/kvfd.2016.16008 .

Milanović, Valentina, Radovanović, Anita, Vasilijić, Sasa, Mrvić, Verica, Milosević, Božidar, "Histological and Immunological Changes in Uterus During the Different Reproductive Stages at Californian Rabbit (Oryctolagus cuniculus)" in Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23, no. 1 (2017):137-144,
https://doi.org/10.9775/kvfd.2016.16008 . .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Milošević, Sandra
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2605
AB  - Introduction. Thyroid hormones (TH) play a pivotal role in the development of mammalian skin and are necessary for both, the initiation and maintenance of hair growth. Hypothyroid rats showed  impaired epidermal proliferation, hair growth, and wound healing. Stem cells located at the bulges of the hair follicles are responsible for hair cycling and contribute to the regeneration of the new epidermis after wounding. Therefore a reduction in the num¬ber or function of the bulge stem cells could be responsible for this phenotype. 
We hypothesized that decrease in TH during prenatal development would affect bulge stem cell population.
Material and methods. Subclinical hypothyroidism was induced with propylthiouracil through drinking water in dose 1.5 mg/l in pregnant Albino Oxford rats from the first day of gravidity. Control group was untreated. Seven-day-old pups were euthanized and skin samples were taken from dorsal part of their bodies. For the immunohistochemical study PCNA (Santa Cruz Biotechnology) and NANOG (Thermo scientific) antibodies were used.
Results. . The number of hair follicles was reduced in hypothyroid pups compared to 
controls. They also had a decreased expression of PCNA in cells of inner and outer sheat of hair follicles, but increased number of NANOG positive bulge stem cells. 
Conclusion. This suggests that re¬duced activation of stem cells leading to their accumulation in the bulges which is associated with reduced stem cell prolif¬eration and mobilization.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
T1  - Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population
SP  - 152
EP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2605
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Milošević, Sandra and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2017",
abstract = "Introduction. Thyroid hormones (TH) play a pivotal role in the development of mammalian skin and are necessary for both, the initiation and maintenance of hair growth. Hypothyroid rats showed  impaired epidermal proliferation, hair growth, and wound healing. Stem cells located at the bulges of the hair follicles are responsible for hair cycling and contribute to the regeneration of the new epidermis after wounding. Therefore a reduction in the num¬ber or function of the bulge stem cells could be responsible for this phenotype. 
We hypothesized that decrease in TH during prenatal development would affect bulge stem cell population.
Material and methods. Subclinical hypothyroidism was induced with propylthiouracil through drinking water in dose 1.5 mg/l in pregnant Albino Oxford rats from the first day of gravidity. Control group was untreated. Seven-day-old pups were euthanized and skin samples were taken from dorsal part of their bodies. For the immunohistochemical study PCNA (Santa Cruz Biotechnology) and NANOG (Thermo scientific) antibodies were used.
Results. . The number of hair follicles was reduced in hypothyroid pups compared to 
controls. They also had a decreased expression of PCNA in cells of inner and outer sheat of hair follicles, but increased number of NANOG positive bulge stem cells. 
Conclusion. This suggests that re¬duced activation of stem cells leading to their accumulation in the bulges which is associated with reduced stem cell prolif¬eration and mobilization.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb",
title = "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population",
pages = "152-152",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2605"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Milošević, S., Kovačević Filipović, M.,& Radovanović, A.. (2017). Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 152-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2605

Lužajić T, Milošević I, Danilović Luković J, Marković D, Milošević S, Kovačević Filipović M, Radovanović A. Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:152-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2605 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Milošević, Sandra, Kovačević Filipović, Milica, Radovanović, Anita, "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):152-152,
https://hdl.handle.net/21.15107/rcub_veterinar_2605 .