TY  - CONF
AU  - Aleksić-Kovačević, Sanja
AU  - Gligorić, Srđan
AU  - Kukolj, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3060
AB  - Ретроспективна и опсервациона анализа података о туморима паса спроведена
је на узорцима тумора достављеним Лабораторији за патологију, Факултета
ветеринарске медицине, Универзитета у Београду од 2017. до 2022. године.
Дескриптивна епидемиолошка анализа обухватала је податке који се односе на:
расу, узраст, пол и особине тумора. Мада се учесталост неоплазми повећава са
старењем, веома мало информација је доступно о појави неоплазми код паса до
12 месеци старости, као и код младих паса у узрасту до 24 месеца.
Наша испитивања указују на присуство различитих типова бенигних и
малигних тумора код младих паса до 12 месеци старости. Представљен је
ретроспективни преглед хистопатолошких дијагноза тумора достављених
нашој лабораторији током последњих пет година код две групе младих паса: у
старосној доби до 12 месеци и код паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 2,26% је
идентификовано код паса до 12 месеци старости и 4,02% код паса старости од
13 до 24 месеца. У старосној групи до 12 месеци, 80% тумора су били бенигни,
а 20% малигни, а код оних узраста 13 до 24 месеца, учесталост бенигних
неоплазми била је 67,35%, док су малигни чинили већ 32,65% свих тумора.
Хистиоцитом коже паса је био најчешће присутан тумор у обе групе младих
паса. Фибросарком је дијагностикован код женке вајмарског птичара, старе
само четири месеца. Неопластични процеси код младих паса најчешће су били
заступљени код расе француски булдог и код мешанаца. Наведена истраживања
из области ветеринарске онколошке патологије, дају драгоцене епидемиолошке
податке о туморима који се јављају код младих паса у Републици Србији.
AB  - According to WHO reports, tumors most often occur in older dogs between 9 and 12
years of age, with different breed predispositions for certain types of neoplasms. A
retrospective and cross-sectional study of canine tumors was performed at Pathology
Department, Faculty of Veterinary Medicine, Belgrade University, from 2017-2022.
Descriptive epidemiology was made from all data: breed, age, sex, and tumor features.
Although the incidence of neoplasms increases with age, little information is available
on the occurrence of neoplasms in dogs up to the age of 12 as well as 24 months.
Current study specimens revealed presence of different types of benign and malignant
tumors in dogs up to 12 months. Retrospective review of histopathological diagnoses
of neoplastic tissue samples submitted in last five years at Laboratory for pathology,
Faculty of veterinary Medicine, University of Belgrade, is performed having special
attention on: group up to the age of 12 months and group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, 2.26% were identified in
dogs up to age of 12 months and 4.02% in dogs aged from 13 to 24 months. In the
present analysis in the group up to 12 months 80% of tumors were benign, and 20%
were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs
aged from 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in
both of groups. Fibrosarcoma have been reported in only four months old female
Weimaraner. French bulldog and mixed-breed dogs were the most frequently affected.
These obtained data in field of veterinary oncology provide valuable epidemiological
information on neoplasms occurring in young dogs in Republic of Serbia.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji
T1  - Epidemiology of tumours in young dogs in Republic of Serbia from 2017-2022
SP  - 128
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3060
ER  - 

@conference{
author = "Aleksić-Kovačević, Sanja and Gligorić, Srđan and Kukolj, Vladimir",
year = "2023",
abstract = "Ретроспективна и опсервациона анализа података о туморима паса спроведена
је на узорцима тумора достављеним Лабораторији за патологију, Факултета
ветеринарске медицине, Универзитета у Београду од 2017. до 2022. године.
Дескриптивна епидемиолошка анализа обухватала је податке који се односе на:
расу, узраст, пол и особине тумора. Мада се учесталост неоплазми повећава са
старењем, веома мало информација је доступно о појави неоплазми код паса до
12 месеци старости, као и код младих паса у узрасту до 24 месеца.
Наша испитивања указују на присуство различитих типова бенигних и
малигних тумора код младих паса до 12 месеци старости. Представљен је
ретроспективни преглед хистопатолошких дијагноза тумора достављених
нашој лабораторији током последњих пет година код две групе младих паса: у
старосној доби до 12 месеци и код паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 2,26% је
идентификовано код паса до 12 месеци старости и 4,02% код паса старости од
13 до 24 месеца. У старосној групи до 12 месеци, 80% тумора су били бенигни,
а 20% малигни, а код оних узраста 13 до 24 месеца, учесталост бенигних
неоплазми била је 67,35%, док су малигни чинили већ 32,65% свих тумора.
Хистиоцитом коже паса је био најчешће присутан тумор у обе групе младих
паса. Фибросарком је дијагностикован код женке вајмарског птичара, старе
само четири месеца. Неопластични процеси код младих паса најчешће су били
заступљени код расе француски булдог и код мешанаца. Наведена истраживања
из области ветеринарске онколошке патологије, дају драгоцене епидемиолошке
податке о туморима који се јављају код младих паса у Републици Србији., According to WHO reports, tumors most often occur in older dogs between 9 and 12
years of age, with different breed predispositions for certain types of neoplasms. A
retrospective and cross-sectional study of canine tumors was performed at Pathology
Department, Faculty of Veterinary Medicine, Belgrade University, from 2017-2022.
Descriptive epidemiology was made from all data: breed, age, sex, and tumor features.
Although the incidence of neoplasms increases with age, little information is available
on the occurrence of neoplasms in dogs up to the age of 12 as well as 24 months.
Current study specimens revealed presence of different types of benign and malignant
tumors in dogs up to 12 months. Retrospective review of histopathological diagnoses
of neoplastic tissue samples submitted in last five years at Laboratory for pathology,
Faculty of veterinary Medicine, University of Belgrade, is performed having special
attention on: group up to the age of 12 months and group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, 2.26% were identified in
dogs up to age of 12 months and 4.02% in dogs aged from 13 to 24 months. In the
present analysis in the group up to 12 months 80% of tumors were benign, and 20%
were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs
aged from 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in
both of groups. Fibrosarcoma have been reported in only four months old female
Weimaraner. French bulldog and mixed-breed dogs were the most frequently affected.
These obtained data in field of veterinary oncology provide valuable epidemiological
information on neoplasms occurring in young dogs in Republic of Serbia.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji, Epidemiology of tumours in young dogs in Republic of Serbia from 2017-2022",
pages = "128-129",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3060"
}

Aleksić-Kovačević, S., Gligorić, S.,& Kukolj, V.. (2023). Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 128-129.
https://hdl.handle.net/21.15107/rcub_veterinar_3060

Aleksić-Kovačević S, Gligorić S, Kukolj V. Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:128-129.
https://hdl.handle.net/21.15107/rcub_veterinar_3060 .

Aleksić-Kovačević, Sanja, Gligorić, Srđan, Kukolj, Vladimir, "Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):128-129,
https://hdl.handle.net/21.15107/rcub_veterinar_3060 .

TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Mirčeta, Jovan
AU  - Djurdjević, Biljana
AU  - Lazić, Sava
AU  - Aleksić-Kovačević, Sanja
AU  - Petrović, Jelena
AU  - Polaček, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2941
AB  - African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.
PB  - MDPI
T2  - Pathogens
T1  - African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia
VL  - 12
IS  - 5
SP  - 691
DO  - 10.3390/pathogens12050691
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Mirčeta, Jovan and Djurdjević, Biljana and Lazić, Sava and Aleksić-Kovačević, Sanja and Petrović, Jelena and Polaček, Vladimir",
year = "2023",
abstract = "African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.",
publisher = "MDPI",
journal = "Pathogens",
title = "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia",
volume = "12",
number = "5",
pages = "691",
doi = "10.3390/pathogens12050691"
}

Prodanov-Radulović, J., Mirčeta, J., Djurdjević, B., Lazić, S., Aleksić-Kovačević, S., Petrović, J.,& Polaček, V.. (2023). African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens
MDPI., 12(5), 691.
https://doi.org/10.3390/pathogens12050691

Prodanov-Radulović J, Mirčeta J, Djurdjević B, Lazić S, Aleksić-Kovačević S, Petrović J, Polaček V. African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens. 2023;12(5):691.
doi:10.3390/pathogens12050691 .

Prodanov-Radulović, Jasna, Mirčeta, Jovan, Djurdjević, Biljana, Lazić, Sava, Aleksić-Kovačević, Sanja, Petrović, Jelena, Polaček, Vladimir, "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia" in Pathogens, 12, no. 5 (2023):691,
https://doi.org/10.3390/pathogens12050691 . .

TY  - JOUR
AU  - Aleksić-Kovačević, Sanja
AU  - Vučićević, Miloš
AU  - Özvegy, József
AU  - Jelisić, Stefan
AU  - Djurdjević, Biljana
AU  - Prodanov-Radulović, Jasna
AU  - Došenović, Milan
AU  - Marinković, Darko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3156
AB  - The eight-year study (2015–2023) was performed on a large sample of poached European
pond turtles infected with Haemogregarina stepanowi and held in a pond that belongs to a quarantine
section of Belgrade Zoo. The protected species of European pond turtles have been found in poor
health, with general weakness, anorexia, and low motility. Comprehensive cytological, hematological,
molecular, and postmortem evaluations have been performed. Initially, Diff Quick staining of the
blood smears revealed rounded or elongated erythrocytes, often bearing premeront or U-shaped
gamont of the hemogregarines inside. The reduced erythrocyte numbers, hemoglobin, and hematocrit
values found in the examined population of infected turtles indicated anemia. Macroscopically, shell
necrosis and massive skin hemorrhages were the most prominent findings observed in diseased
turtles. Microscopically, the lungs, liver, kidneys, and spleen revealed hyperemia, hemorrhages,
and the presence of parasitic stages in tissue samples in 31 of 40 necropsied turtles. Cytological
and microscopic examination of the samples proved to be sufficient for establishing the infection,
but molecular analyses of the 18S sequence were used for phylogenetic studies. Over the years, the
number of diseased and dead turtles has decreased, which could be hypothetically attributed to the
elimination of leeches as the definitive host.
PB  - MDPI
T2  - Animals
T1  - Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine
VL  - 13
IS  - 15
SP  - 2429
DO  - 10.3390/ani13152429
ER  - 

@article{
author = "Aleksić-Kovačević, Sanja and Vučićević, Miloš and Özvegy, József and Jelisić, Stefan and Djurdjević, Biljana and Prodanov-Radulović, Jasna and Došenović, Milan and Marinković, Darko",
year = "2023",
abstract = "The eight-year study (2015–2023) was performed on a large sample of poached European
pond turtles infected with Haemogregarina stepanowi and held in a pond that belongs to a quarantine
section of Belgrade Zoo. The protected species of European pond turtles have been found in poor
health, with general weakness, anorexia, and low motility. Comprehensive cytological, hematological,
molecular, and postmortem evaluations have been performed. Initially, Diff Quick staining of the
blood smears revealed rounded or elongated erythrocytes, often bearing premeront or U-shaped
gamont of the hemogregarines inside. The reduced erythrocyte numbers, hemoglobin, and hematocrit
values found in the examined population of infected turtles indicated anemia. Macroscopically, shell
necrosis and massive skin hemorrhages were the most prominent findings observed in diseased
turtles. Microscopically, the lungs, liver, kidneys, and spleen revealed hyperemia, hemorrhages,
and the presence of parasitic stages in tissue samples in 31 of 40 necropsied turtles. Cytological
and microscopic examination of the samples proved to be sufficient for establishing the infection,
but molecular analyses of the 18S sequence were used for phylogenetic studies. Over the years, the
number of diseased and dead turtles has decreased, which could be hypothetically attributed to the
elimination of leeches as the definitive host.",
publisher = "MDPI",
journal = "Animals",
title = "Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine",
volume = "13",
number = "15",
pages = "2429",
doi = "10.3390/ani13152429"
}

Aleksić-Kovačević, S., Vučićević, M., Özvegy, J., Jelisić, S., Djurdjević, B., Prodanov-Radulović, J., Došenović, M.,& Marinković, D.. (2023). Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine. in Animals
MDPI., 13(15), 2429.
https://doi.org/10.3390/ani13152429

Aleksić-Kovačević S, Vučićević M, Özvegy J, Jelisić S, Djurdjević B, Prodanov-Radulović J, Došenović M, Marinković D. Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine. in Animals. 2023;13(15):2429.
doi:10.3390/ani13152429 .

Aleksić-Kovačević, Sanja, Vučićević, Miloš, Özvegy, József, Jelisić, Stefan, Djurdjević, Biljana, Prodanov-Radulović, Jasna, Došenović, Milan, Marinković, Darko, "Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine" in Animals, 13, no. 15 (2023):2429,
https://doi.org/10.3390/ani13152429 . .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Labus, Tatjana
AU  - Nešić, Slađan
AU  - Vučićević, Miloš
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3100
AB  - Zarazne bolesti su jedan od vodećih uzroka ekonomskih gubitaka
u živinarskoj proizvodnji. Živina je podložna različitim zaraznim bolestima
s obzirom na specifičan način uzgoja. Slobodno držanje živine,
kao vid uzgoja, karakteriše se slabim biosigurnosnim merama. Jedinke
u takvom načinu držanja mogu doći u kontakt i sa drugim vrstama
ptica, uključujući i migratorne vrste. Kod farmskog načina uzgoja
živine, biosigurnosne mere su na višem nivou, ali je visok proizvodni
pritisak uticao da jedinke postanu podložnije mnogim bolestima. Najznačajnije
zarazne bolesti živine koje su obavezne za prijavljivanje
nadležnim organima su atipična kuga živine, gamboro bolest, avijarna
influenca, infektivni laringotraheitis, tuberkuloza, Marekova bolest i
salmoneloza. U cilju sprečavanja pojave i kontrole bolesti kod živine,
kao i kod drugih životinjskih vrsta postupa se prema Zakonu o veterinarstvu
(“Službeni glasnik RS”, broj 91/05) i odgovarajućim podzakonskim
aktima. Radi otkrivanja uzroka uginuća, pored ostalih dijagnostičkih
procedura, vrši se i obdukcija leševa živine prema standardnim
protokolima za obdukciju ptica. Ukoliko je potrebno uraditi patohistološku
analizu, uzorci ne smeju biti deblji od 0,5 cm kako bi 10% puferizovani
formalin prodro kroz tkivo u procesu fiksacije tokom najmanje
48 časova. Nakon obdukcije, leševe živine treba neškodljivo ukloniti.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
T1  - Zarazne bolesti živine obavezne za prijavljivanje – klinička slika, patomorfološke promene i zakonski propisi / tehnika obdukcije živine i slanje materijala
SP  - 145
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3100
ER  - 

@conference{
author = "Vučićević, Ivana and Labus, Tatjana and Nešić, Slađan and Vučićević, Miloš and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "Zarazne bolesti su jedan od vodećih uzroka ekonomskih gubitaka
u živinarskoj proizvodnji. Živina je podložna različitim zaraznim bolestima
s obzirom na specifičan način uzgoja. Slobodno držanje živine,
kao vid uzgoja, karakteriše se slabim biosigurnosnim merama. Jedinke
u takvom načinu držanja mogu doći u kontakt i sa drugim vrstama
ptica, uključujući i migratorne vrste. Kod farmskog načina uzgoja
živine, biosigurnosne mere su na višem nivou, ali je visok proizvodni
pritisak uticao da jedinke postanu podložnije mnogim bolestima. Najznačajnije
zarazne bolesti živine koje su obavezne za prijavljivanje
nadležnim organima su atipična kuga živine, gamboro bolest, avijarna
influenca, infektivni laringotraheitis, tuberkuloza, Marekova bolest i
salmoneloza. U cilju sprečavanja pojave i kontrole bolesti kod živine,
kao i kod drugih životinjskih vrsta postupa se prema Zakonu o veterinarstvu
(“Službeni glasnik RS”, broj 91/05) i odgovarajućim podzakonskim
aktima. Radi otkrivanja uzroka uginuća, pored ostalih dijagnostičkih
procedura, vrši se i obdukcija leševa živine prema standardnim
protokolima za obdukciju ptica. Ukoliko je potrebno uraditi patohistološku
analizu, uzorci ne smeju biti deblji od 0,5 cm kako bi 10% puferizovani
formalin prodro kroz tkivo u procesu fiksacije tokom najmanje
48 časova. Nakon obdukcije, leševe živine treba neškodljivo ukloniti.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023",
title = "Zarazne bolesti živine obavezne za prijavljivanje – klinička slika, patomorfološke promene i zakonski propisi / tehnika obdukcije živine i slanje materijala",
pages = "145-156",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3100"
}

Vučićević, I., Labus, T., Nešić, S., Vučićević, M.,& Aleksić-Kovačević, S.. (2023). Zarazne bolesti živine obavezne za prijavljivanje – klinička slika, patomorfološke promene i zakonski propisi / tehnika obdukcije živine i slanje materijala. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 145-156.
https://hdl.handle.net/21.15107/rcub_veterinar_3100

Vučićević I, Labus T, Nešić S, Vučićević M, Aleksić-Kovačević S. Zarazne bolesti živine obavezne za prijavljivanje – klinička slika, patomorfološke promene i zakonski propisi / tehnika obdukcije živine i slanje materijala. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023. 2023;:145-156.
https://hdl.handle.net/21.15107/rcub_veterinar_3100 .

Vučićević, Ivana, Labus, Tatjana, Nešić, Slađan, Vučićević, Miloš, Aleksić-Kovačević, Sanja, "Zarazne bolesti živine obavezne za prijavljivanje – klinička slika, patomorfološke promene i zakonski propisi / tehnika obdukcije živine i slanje materijala" in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023 (2023):145-156,
https://hdl.handle.net/21.15107/rcub_veterinar_3100 .

TY  - CONF
AU  - Đurđević, Biljana
AU  - Vučićević, Ivana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Knežević, Slobodan
AU  - Petrović, Tamaš
AU  - Grubač, Siniša
AU  - Gajdov, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3365
AB  - The highly pathogenic avian influenza (HPAI) epidemic in the 2021/2022 epidemiological year was the most
widespread in Europe, and HPAI H5N1 was by far the predominant virus type reported. In Serbia, since early
2021 and 2022, multiple outbreaks of HPAI have occurred. The H5N1 subtype was also dominant during this
epizootic, although a few cases of H5N8 subtype infection were reported in mute swans. This epizootic
affected backyard chickens (3 outbreaks), and the virus was also detected in wild birds, mostly in mute swans
(4 outbreaks). All cases were reported in the north of Serbia. Here we describe the pathological findings of
natural HPAI H5N1 infection in poultry species (chickens and turkeys) and wild birds (mute swans) that died
during this epizootic. Routine necropsies were carried out on the carcasses of 15 mute swans, 15 chickens,
and four turkeys. The external body and internal organs were examined grossly, and the gross pathology was
recorded and photographed. Body condition was estimated based on the amount of body fat and
musculature. Tissue samples of the brain, pancreas, spleen, and lungs were collected, and the supernatants
of the tissue homogenates were used for molecular diagnosis by RT-qPCR method. The affected birds showed
nervous manifestations (abnormal head position, tremors, leg paralysis) and all birds were in good condition.
The presence of influenza virus was detected in tissue samples of all tested animals. The external macroscopic
changes included cyanosis and necrosis in the crest and wattle, and these lesions were more pronounced in
chickens. In mute swans, there were no external lesions. The H5N1 HPAI virus produced several consistent
gross lesions among the species investigated. Foremost among these lesions was: multifocal pancreatic
necrosis and hemorrhages, petechial hemorrhages in coelomic and epicardial fat and epicardial petechiae.
The lungs showed moderate (turkeys) to severe (chickens and mute swans) diffuse congestion and oedema.
In most cases, mild splenomegaly and spleen necrosis were noted. Additionally, gizzard or proventricular
lesions were not observed in any bird. The natural HPAI H5N1 infection in poultry and mute swans showed
similar clinical disease, including neurological disorders, as well as similar pathologic presentation involving
necrotic lesions and vascular damage, primarily affecting the pancreas and myocardium. Evaluating the
pathological presentation of natural disease is particularly important in emerging infectious diseases such as
influenza A virus, in which different strains can have different pathogenicity and clinical presentations.
PB  - Scientific Veterinary Institute Novi Sad
C3  - 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023
T1  - Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3365
ER  - 

@conference{
author = "Đurđević, Biljana and Vučićević, Ivana and Polaček, Vladimir and Pajić, Marko and Knežević, Slobodan and Petrović, Tamaš and Grubač, Siniša and Gajdov, Vladimir and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "The highly pathogenic avian influenza (HPAI) epidemic in the 2021/2022 epidemiological year was the most
widespread in Europe, and HPAI H5N1 was by far the predominant virus type reported. In Serbia, since early
2021 and 2022, multiple outbreaks of HPAI have occurred. The H5N1 subtype was also dominant during this
epizootic, although a few cases of H5N8 subtype infection were reported in mute swans. This epizootic
affected backyard chickens (3 outbreaks), and the virus was also detected in wild birds, mostly in mute swans
(4 outbreaks). All cases were reported in the north of Serbia. Here we describe the pathological findings of
natural HPAI H5N1 infection in poultry species (chickens and turkeys) and wild birds (mute swans) that died
during this epizootic. Routine necropsies were carried out on the carcasses of 15 mute swans, 15 chickens,
and four turkeys. The external body and internal organs were examined grossly, and the gross pathology was
recorded and photographed. Body condition was estimated based on the amount of body fat and
musculature. Tissue samples of the brain, pancreas, spleen, and lungs were collected, and the supernatants
of the tissue homogenates were used for molecular diagnosis by RT-qPCR method. The affected birds showed
nervous manifestations (abnormal head position, tremors, leg paralysis) and all birds were in good condition.
The presence of influenza virus was detected in tissue samples of all tested animals. The external macroscopic
changes included cyanosis and necrosis in the crest and wattle, and these lesions were more pronounced in
chickens. In mute swans, there were no external lesions. The H5N1 HPAI virus produced several consistent
gross lesions among the species investigated. Foremost among these lesions was: multifocal pancreatic
necrosis and hemorrhages, petechial hemorrhages in coelomic and epicardial fat and epicardial petechiae.
The lungs showed moderate (turkeys) to severe (chickens and mute swans) diffuse congestion and oedema.
In most cases, mild splenomegaly and spleen necrosis were noted. Additionally, gizzard or proventricular
lesions were not observed in any bird. The natural HPAI H5N1 infection in poultry and mute swans showed
similar clinical disease, including neurological disorders, as well as similar pathologic presentation involving
necrotic lesions and vascular damage, primarily affecting the pancreas and myocardium. Evaluating the
pathological presentation of natural disease is particularly important in emerging infectious diseases such as
influenza A virus, in which different strains can have different pathogenicity and clinical presentations.",
publisher = "Scientific Veterinary Institute Novi Sad",
journal = "15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023",
title = "Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3365"
}

Đurđević, B., Vučićević, I., Polaček, V., Pajić, M., Knežević, S., Petrović, T., Grubač, S., Gajdov, V.,& Aleksić-Kovačević, S.. (2023). Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year. in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023
Scientific Veterinary Institute Novi Sad..
https://hdl.handle.net/21.15107/rcub_veterinar_3365

Đurđević B, Vučićević I, Polaček V, Pajić M, Knežević S, Petrović T, Grubač S, Gajdov V, Aleksić-Kovačević S. Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year. in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023. 2023;.
https://hdl.handle.net/21.15107/rcub_veterinar_3365 .

Đurđević, Biljana, Vučićević, Ivana, Polaček, Vladimir, Pajić, Marko, Knežević, Slobodan, Petrović, Tamaš, Grubač, Siniša, Gajdov, Vladimir, Aleksić-Kovačević, Sanja, "Pathology associated with highly pathogenic avian influenza H5N1 in naturally infected birds in Serbia in the 2021/2022 epidemiological year" in 15th EPIZONE Annual Meeting, New perspectives for the new era, Novi sad, April 26 - 28, 2023 (2023),
https://hdl.handle.net/21.15107/rcub_veterinar_3365 .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Petrović, Tamaš
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2706
AB  - In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.
PB  - MDPI
T2  - Animals
T1  - Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
VL  - 13
IS  - 4
SP  - 700
DO  - 10.3390/ani13040700
ER  - 

@article{
author = "Đurđević, Biljana and Polaček, Vladimir and Pajić, Marko and Petrović, Tamaš and Vučićević, Ivana and Vidanović, Dejan and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.",
publisher = "MDPI",
journal = "Animals",
title = "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia",
volume = "13",
number = "4",
pages = "700",
doi = "10.3390/ani13040700"
}

Đurđević, B., Polaček, V., Pajić, M., Petrović, T., Vučićević, I., Vidanović, D.,& Aleksić-Kovačević, S.. (2023). Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals
MDPI., 13(4), 700.
https://doi.org/10.3390/ani13040700

Đurđević B, Polaček V, Pajić M, Petrović T, Vučićević I, Vidanović D, Aleksić-Kovačević S. Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals. 2023;13(4):700.
doi:10.3390/ani13040700 .

Đurđević, Biljana, Polaček, Vladimir, Pajić, Marko, Petrović, Tamaš, Vučićević, Ivana, Vidanović, Dejan, Aleksić-Kovačević, Sanja, "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia" in Animals, 13, no. 4 (2023):700,
https://doi.org/10.3390/ani13040700 . .

TY  - CONF
AU  - Aleksić Kovačević, Sanja
AU  - Gligorić, Srđan
AU  - Kukolj, Vladimir
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3579
AB  - Тумори паса представљају најчешћу дијагнозу у узорцима који су
достављени Лабораторији за патологију, Факултета ветеринарске
медицине, Универзитета у Београду. Учесталост неоплазми повећава
се са старењем, ипак неки тумори се чешће јављају код млађих паса
мада је доступно мало информација о појави неоплазми код паса
до 12 месеци старости.
У овој студији били су присутни различити типови бенигних и
малигних тумора код веома младих паса до 12 месеци старости.
Представљен је ретроспективни преглед хистопатолошких дијагноза
тумора у нашој лабораторији током последњих пет година (2017-
2021) код две групе младих паса: у старосној доби до 12 месеци и
паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 55
(2,26%) је идентификовано код паса до 12 месеци старости и 98
(4,02%) код паса старости од 13 до 24 месеца. Хистиоцитом коже
паса је био најчешће присутан тумор у обе групе младих паса: 54,55%
код паса до 12 месеци, а затим 24,49% код паса узраста 13 до 24
месеца. Неоплазме које нису припадале хистиоцитомима, груписане
су као бенигни епителни, малигни епителни, хематопоетски, бенигни
мезенхимски и малигни мезенхимски тумори. У старосној групи до 12 месеци, 80% тумора су били бенигни, а 20% малигни, а код
оних узраста 13 до 24 месеца, учесталост бенигних неоплазми
била је 67,35%, док су малигни чинили већ 32,65% свих тумора у
овој старосној категорији. Најмлађи пси у нашој студији били су
двомесечни мужјак и женка бишона са субкутаним мастоцитомом
и папиломом, међутим фибросарком је дијагностикован код женке
вајмарског птичара, старе само четири месеца. Пси расе француски
булдог и пси мешанци су најчешће били погођени неопластичним
процесима. Ови подаци дају драгоцене епидемиолошке податке о
туморима који се јављају код младих паса у Републици Србији.
AB  - Neoplasia in dogs are the most frequent diagnosis in histological submissions at Pathology Department, Faculty of Veterinary Medicine,
Belgrade University. Although incidence of neoplasms increases with
age some tumors occur more commonly in younger dogs but little
information is available on the occurrence of neoplasms in dogs up to
the age of 12 months.
In our study specimens reveal different types of benign and malignant
tumors in very young dogs up to 12 months. This is a retrospective
review of histopathological diagnoses of neoplasia at our laboratory
during last five years (2017-2021) in two groups of dogs: group up to
the age of 12 months as well as group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, n=55 (2.26%)
were identified in dogs up to age of 12 months and n=98 (4.02%) in
dogs aged from 13 to 24 months. Canine cutaneous histiocytoma was
the most frequent in both of groups: (n=30; 54.55%) in the dogs up
to 12 months followed by (n=24; 24.49%) in those up to 24 months.
Neoplasms other than histiocytoma were grouped as benign epithelial,
malignant epithelial, hematopoietic, benign mesenchymal and malignant
mesenchymal tumors. In the present analysis in the group up to 12
months 80% of tumors were benign, and 20% were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs aged from
13 to 24 months. The youngest dogs in our study were two months old
Bichons male and female with subcutaneous mastocytoma and papilloma respectively, however fibrosarcoma have been reported in only
four months old female Weimaraner. French bulldog and mixed-breed
dogs were the most frequently affected. These data provide valuable
epidemiological information on neoplasms occurring in juvenile dogs
in Republic of Serbia.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
T1  - Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji
SP  - 189
EP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3579
ER  - 

@conference{
author = "Aleksić Kovačević, Sanja and Gligorić, Srđan and Kukolj, Vladimir",
year = "2022",
abstract = "Тумори паса представљају најчешћу дијагнозу у узорцима који су
достављени Лабораторији за патологију, Факултета ветеринарске
медицине, Универзитета у Београду. Учесталост неоплазми повећава
се са старењем, ипак неки тумори се чешће јављају код млађих паса
мада је доступно мало информација о појави неоплазми код паса
до 12 месеци старости.
У овој студији били су присутни различити типови бенигних и
малигних тумора код веома младих паса до 12 месеци старости.
Представљен је ретроспективни преглед хистопатолошких дијагноза
тумора у нашој лабораторији током последњих пет година (2017-
2021) код две групе младих паса: у старосној доби до 12 месеци и
паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 55
(2,26%) је идентификовано код паса до 12 месеци старости и 98
(4,02%) код паса старости од 13 до 24 месеца. Хистиоцитом коже
паса је био најчешће присутан тумор у обе групе младих паса: 54,55%
код паса до 12 месеци, а затим 24,49% код паса узраста 13 до 24
месеца. Неоплазме које нису припадале хистиоцитомима, груписане
су као бенигни епителни, малигни епителни, хематопоетски, бенигни
мезенхимски и малигни мезенхимски тумори. У старосној групи до 12 месеци, 80% тумора су били бенигни, а 20% малигни, а код
оних узраста 13 до 24 месеца, учесталост бенигних неоплазми
била је 67,35%, док су малигни чинили већ 32,65% свих тумора у
овој старосној категорији. Најмлађи пси у нашој студији били су
двомесечни мужјак и женка бишона са субкутаним мастоцитомом
и папиломом, међутим фибросарком је дијагностикован код женке
вајмарског птичара, старе само четири месеца. Пси расе француски
булдог и пси мешанци су најчешће били погођени неопластичним
процесима. Ови подаци дају драгоцене епидемиолошке податке о
туморима који се јављају код младих паса у Републици Србији., Neoplasia in dogs are the most frequent diagnosis in histological submissions at Pathology Department, Faculty of Veterinary Medicine,
Belgrade University. Although incidence of neoplasms increases with
age some tumors occur more commonly in younger dogs but little
information is available on the occurrence of neoplasms in dogs up to
the age of 12 months.
In our study specimens reveal different types of benign and malignant
tumors in very young dogs up to 12 months. This is a retrospective
review of histopathological diagnoses of neoplasia at our laboratory
during last five years (2017-2021) in two groups of dogs: group up to
the age of 12 months as well as group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, n=55 (2.26%)
were identified in dogs up to age of 12 months and n=98 (4.02%) in
dogs aged from 13 to 24 months. Canine cutaneous histiocytoma was
the most frequent in both of groups: (n=30; 54.55%) in the dogs up
to 12 months followed by (n=24; 24.49%) in those up to 24 months.
Neoplasms other than histiocytoma were grouped as benign epithelial,
malignant epithelial, hematopoietic, benign mesenchymal and malignant
mesenchymal tumors. In the present analysis in the group up to 12
months 80% of tumors were benign, and 20% were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs aged from
13 to 24 months. The youngest dogs in our study were two months old
Bichons male and female with subcutaneous mastocytoma and papilloma respectively, however fibrosarcoma have been reported in only
four months old female Weimaraner. French bulldog and mixed-breed
dogs were the most frequently affected. These data provide valuable
epidemiological information on neoplasms occurring in juvenile dogs
in Republic of Serbia.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022",
title = "Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji",
pages = "189-192",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3579"
}

Aleksić Kovačević, S., Gligorić, S.,& Kukolj, V.. (2022). Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
Banja Luka : Veterinarska komora Republike Srpske., 189-192.
https://hdl.handle.net/21.15107/rcub_veterinar_3579

Aleksić Kovačević S, Gligorić S, Kukolj V. Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022. 2022;:189-192.
https://hdl.handle.net/21.15107/rcub_veterinar_3579 .

Aleksić Kovačević, Sanja, Gligorić, Srđan, Kukolj, Vladimir, "Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022 (2022):189-192,
https://hdl.handle.net/21.15107/rcub_veterinar_3579 .

TY  - CONF
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
AU  - Jelisić, Stefan
AU  - Aleksić-Kovačević, Sanja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2426
AB  - Ovčija svrabež (Scrapie, engl.) je neurodegenerativno oboljenje ovaca iz grupe transmisivnih spongiformnih encefalopatija (TSE).
Oboljenja iz grupe TSE se nazivaju prionske bolesti, jer je patološki
prion protein etiološki faktor za razvoj ovih oboljenja. Ovčija svrabež
se javlja u dve forme, kao klasična i atipična. Osetljivost ovaca na ovčiju svrabež zavisi od polimorfizma gena prionskog proteina i prirode
uzročnika prionske bolesti. Kodoni 136, 154 i 171 gena prion proteina
određuju stepen otpornosti, tj. sklonosti za Scrapie. Pošto je svaki alel
obeležen troslovnim kodom, utvrđeno je da su jedinke sa ARR alelom
visoko rezistentne na klasičnu ovčiju svrabež, ali ne pruža nikakvu posebnu zaštitu protiv atipične forme Scrapie-a. Primenom qPCR metode može se sprovesti genotipizacija jedinki u cilju uspešne selekcije
rezistentnih jedinki na ovčiju svrabež. Za metodu je neophodno uzeti
odgovarajući materijal i pripremiti ga za određivanje alela. Određivanje alela qPCR tehnikom sa Taquman probom je moguće iz krvi sa ili
bez dodatka antikoagulansa. Za dijagnostiku prisustva patološkog prion proteina kod ovaca u Laboratoriji za patologiju primenjuje se ELISA
metoda. Neophodno tkivo za dijagnostiku patološkog prion proteina
je obeks produžene moždine. Produžena moždina ovaca se uzorkuje
kroz foramen magnum specijalizovanim kašikama za uzorkovanja tkiva malih preživara
AB  - Scrapie is a neurodegenerative disease belonging to the group of transmissible
spongiform encephalopathies (TSE). Diseases from the TSE group are also known as prion
diseases because they are caused by the pathological prion protein. Scrapie is a disease
that has two forms, classical and atypical. The susceptibility of sheep to Scrapie depends
on the polymorphism of the prion protein gene and the nature of the agent causing prion
disease. Codons 136, 154 and 171 of the prion protein gene determine a certain degree
of resistance, ie the tendency for Scrapie. Since each allele is marked with a three-letter
code, individuals with the ARR allele were found to be highly resistant to classical Scrapie,
but did not provide any special protection against the atypical form. Using the qPCR
method, genotyping of individuals can be performed for the successful selection of Scrapie
resistant animals. It is necessary to sample the appropriate material for qPCR method and
to prepare it for the determination of alleles. Determination of alleles by qPCR technique
with Taquman test is possible from blood with or without anticoagulants. In the Laboratory
of Pathology, the ELISA method is used to diagnose the presence of pathological prion
protein in sheep. The Material necessary for the diagnosis of pathological prion protein
is the region of obex of medulla oblongata. The medulla oblongata of sheep is sampled
through the foramen magnum with specialized spoons for sampling the tissues of small
ruminants.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Otpornost ovaca na ovčiju svrabež i postupak uzorkovanja materijala za dijagnostiku patološkog prion proteina kod ovaca
T1  - Sheep resistance to scrapie and sampling procedure for diagnosis of pathological prion protein in sheep
SP  - 161
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2426
ER  - 

@conference{
author = "Nešić, Slađan and Vučićević, Ivana and Jelisić, Stefan and Aleksić-Kovačević, Sanja",
year = "2022",
abstract = "Ovčija svrabež (Scrapie, engl.) je neurodegenerativno oboljenje ovaca iz grupe transmisivnih spongiformnih encefalopatija (TSE).
Oboljenja iz grupe TSE se nazivaju prionske bolesti, jer je patološki
prion protein etiološki faktor za razvoj ovih oboljenja. Ovčija svrabež
se javlja u dve forme, kao klasična i atipična. Osetljivost ovaca na ovčiju svrabež zavisi od polimorfizma gena prionskog proteina i prirode
uzročnika prionske bolesti. Kodoni 136, 154 i 171 gena prion proteina
određuju stepen otpornosti, tj. sklonosti za Scrapie. Pošto je svaki alel
obeležen troslovnim kodom, utvrđeno je da su jedinke sa ARR alelom
visoko rezistentne na klasičnu ovčiju svrabež, ali ne pruža nikakvu posebnu zaštitu protiv atipične forme Scrapie-a. Primenom qPCR metode može se sprovesti genotipizacija jedinki u cilju uspešne selekcije
rezistentnih jedinki na ovčiju svrabež. Za metodu je neophodno uzeti
odgovarajući materijal i pripremiti ga za određivanje alela. Određivanje alela qPCR tehnikom sa Taquman probom je moguće iz krvi sa ili
bez dodatka antikoagulansa. Za dijagnostiku prisustva patološkog prion proteina kod ovaca u Laboratoriji za patologiju primenjuje se ELISA
metoda. Neophodno tkivo za dijagnostiku patološkog prion proteina
je obeks produžene moždine. Produžena moždina ovaca se uzorkuje
kroz foramen magnum specijalizovanim kašikama za uzorkovanja tkiva malih preživara, Scrapie is a neurodegenerative disease belonging to the group of transmissible
spongiform encephalopathies (TSE). Diseases from the TSE group are also known as prion
diseases because they are caused by the pathological prion protein. Scrapie is a disease
that has two forms, classical and atypical. The susceptibility of sheep to Scrapie depends
on the polymorphism of the prion protein gene and the nature of the agent causing prion
disease. Codons 136, 154 and 171 of the prion protein gene determine a certain degree
of resistance, ie the tendency for Scrapie. Since each allele is marked with a three-letter
code, individuals with the ARR allele were found to be highly resistant to classical Scrapie,
but did not provide any special protection against the atypical form. Using the qPCR
method, genotyping of individuals can be performed for the successful selection of Scrapie
resistant animals. It is necessary to sample the appropriate material for qPCR method and
to prepare it for the determination of alleles. Determination of alleles by qPCR technique
with Taquman test is possible from blood with or without anticoagulants. In the Laboratory
of Pathology, the ELISA method is used to diagnose the presence of pathological prion
protein in sheep. The Material necessary for the diagnosis of pathological prion protein
is the region of obex of medulla oblongata. The medulla oblongata of sheep is sampled
through the foramen magnum with specialized spoons for sampling the tissues of small
ruminants.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Otpornost ovaca na ovčiju svrabež i postupak uzorkovanja materijala za dijagnostiku patološkog prion proteina kod ovaca, Sheep resistance to scrapie and sampling procedure for diagnosis of pathological prion protein in sheep",
pages = "161-167",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2426"
}

Nešić, S., Vučićević, I., Jelisić, S.,& Aleksić-Kovačević, S.. (2022). Otpornost ovaca na ovčiju svrabež i postupak uzorkovanja materijala za dijagnostiku patološkog prion proteina kod ovaca. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 161-167.
https://hdl.handle.net/21.15107/rcub_veterinar_2426

Nešić S, Vučićević I, Jelisić S, Aleksić-Kovačević S. Otpornost ovaca na ovčiju svrabež i postupak uzorkovanja materijala za dijagnostiku patološkog prion proteina kod ovaca. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:161-167.
https://hdl.handle.net/21.15107/rcub_veterinar_2426 .

Nešić, Slađan, Vučićević, Ivana, Jelisić, Stefan, Aleksić-Kovačević, Sanja, "Otpornost ovaca na ovčiju svrabež i postupak uzorkovanja materijala za dijagnostiku patološkog prion proteina kod ovaca" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):161-167,
https://hdl.handle.net/21.15107/rcub_veterinar_2426 .

TY  - CONF
AU  - Salai, Romina
AU  - Kiškarolj, Ferenc
AU  - Aničić, Milan
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
AU  - Marinković, Darko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2924
AB  - У раду су приказани налази патоморфолошког (макроскопског и
хистопатолошког) прегледа угинулих животиња пореклом из Зоо врта Палић са
припадајућим микробиолошким резултатима. Обдукције угинулих животиња и
микробиолошки прегледи су извршени у Ветеринарском специјалистичком
институту „Суботица“, док су хистопатолошка испитивања ткивних узорака
урађена на Катедри за патологију Факултета ветеринарске медицине у Београду.
Приказани су морфолошки и микробиолошки налази код укупно 23
појединачних случајева 20 различитих врста птица и сисара. Доминантани
патоморфолошки налази били су заступљени у форми инфламаторних,
дегенеративно-некротичних и неопластичних промена у гастроинтестиналном
систему, бубрезима и плућима, док је микробиолошким испитивањима
установљено присуство бактерија из рода Escherichia, Salmonella, Pasteurella,
Pseudomonas, Streptococcus i Staphylococcus и гљивица из рода Aspergillus.
Иако су дивље животиње у зоо врту прилично адаптиране на услове средине у
којима живе, узроци обољења и угинућа животиња су делимично последица
измењених услова живота у којима бораве у зоо врту а који се најчешће значајно
разликују од услова природног станишта у дивљини.
AB  - Pathomorphological findings (macroscopic and histopathologic) with corresponding
microbiological results originated from animals died in Zoo Palić were presented in
this paper.
Necropsies and microbiological examinations were performed in Veterinary
Specialist Institute Subotica, while histopathological examination of tissue samples
were performed on Department of Pathology, Faculty of Veterinary Medicine in
Belgrade.
Morphological and microbiological findings of twenty-three cases originated from 20
different avian and mammalian species were shown.
Dominant pathomorphological findings were in form of inflammatory, degenerativenecrotic and neoplastic changes of gastrointestinal system, kidneys and lungs, while
microbiological examination revealed presence of bacteria from genus Escherichia,
Salmonella, Pasteurella, Pseudomonas, Streptococcus and Staphylococcus and
fungus from genus Aspergillus.
Although wild animals in Zoo are pretty adapted on the living conditions, the causes
of their death still are partly due to altered conditions in Zoo which in most cases are
significantly different from the natural wild habita
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
T1  - Prikaz patomorfoloških i laboratorijskih nalaza kod životinja iz Zoo vrta Palić uginulih u periodu od 2017. do 2021. godine
SP  - 50
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2924
ER  - 

@conference{
author = "Salai, Romina and Kiškarolj, Ferenc and Aničić, Milan and Vučićević, Ivana and Aleksić-Kovačević, Sanja and Marinković, Darko",
year = "2022",
abstract = "У раду су приказани налази патоморфолошког (макроскопског и
хистопатолошког) прегледа угинулих животиња пореклом из Зоо врта Палић са
припадајућим микробиолошким резултатима. Обдукције угинулих животиња и
микробиолошки прегледи су извршени у Ветеринарском специјалистичком
институту „Суботица“, док су хистопатолошка испитивања ткивних узорака
урађена на Катедри за патологију Факултета ветеринарске медицине у Београду.
Приказани су морфолошки и микробиолошки налази код укупно 23
појединачних случајева 20 различитих врста птица и сисара. Доминантани
патоморфолошки налази били су заступљени у форми инфламаторних,
дегенеративно-некротичних и неопластичних промена у гастроинтестиналном
систему, бубрезима и плућима, док је микробиолошким испитивањима
установљено присуство бактерија из рода Escherichia, Salmonella, Pasteurella,
Pseudomonas, Streptococcus i Staphylococcus и гљивица из рода Aspergillus.
Иако су дивље животиње у зоо врту прилично адаптиране на услове средине у
којима живе, узроци обољења и угинућа животиња су делимично последица
измењених услова живота у којима бораве у зоо врту а који се најчешће значајно
разликују од услова природног станишта у дивљини., Pathomorphological findings (macroscopic and histopathologic) with corresponding
microbiological results originated from animals died in Zoo Palić were presented in
this paper.
Necropsies and microbiological examinations were performed in Veterinary
Specialist Institute Subotica, while histopathological examination of tissue samples
were performed on Department of Pathology, Faculty of Veterinary Medicine in
Belgrade.
Morphological and microbiological findings of twenty-three cases originated from 20
different avian and mammalian species were shown.
Dominant pathomorphological findings were in form of inflammatory, degenerativenecrotic and neoplastic changes of gastrointestinal system, kidneys and lungs, while
microbiological examination revealed presence of bacteria from genus Escherichia,
Salmonella, Pasteurella, Pseudomonas, Streptococcus and Staphylococcus and
fungus from genus Aspergillus.
Although wild animals in Zoo are pretty adapted on the living conditions, the causes
of their death still are partly due to altered conditions in Zoo which in most cases are
significantly different from the natural wild habita",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022",
title = "Prikaz patomorfoloških i laboratorijskih nalaza kod životinja iz Zoo vrta Palić uginulih u periodu od 2017. do 2021. godine",
pages = "50-51",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2924"
}

Salai, R., Kiškarolj, F., Aničić, M., Vučićević, I., Aleksić-Kovačević, S.,& Marinković, D.. (2022). Prikaz patomorfoloških i laboratorijskih nalaza kod životinja iz Zoo vrta Palić uginulih u periodu od 2017. do 2021. godine. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 50-51.
https://hdl.handle.net/21.15107/rcub_veterinar_2924

Salai R, Kiškarolj F, Aničić M, Vučićević I, Aleksić-Kovačević S, Marinković D. Prikaz patomorfoloških i laboratorijskih nalaza kod životinja iz Zoo vrta Palić uginulih u periodu od 2017. do 2021. godine. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022. 2022;:50-51.
https://hdl.handle.net/21.15107/rcub_veterinar_2924 .

Salai, Romina, Kiškarolj, Ferenc, Aničić, Milan, Vučićević, Ivana, Aleksić-Kovačević, Sanja, Marinković, Darko, "Prikaz patomorfoloških i laboratorijskih nalaza kod životinja iz Zoo vrta Palić uginulih u periodu od 2017. do 2021. godine" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022 (2022):50-51,
https://hdl.handle.net/21.15107/rcub_veterinar_2924 .

TY  - CONF
AU  - Đurđević, Biljana
AU  - Pajić, Marko
AU  - Polaček, Vladimir
AU  - Knežević, Slobodan
AU  - Ratajac, Ratko
AU  - Mihaljev, Žarko
AU  - Aleksić-Kovačević, Sanja
AU  - Vučićević, Ivana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3361
AB  - The eastern imperial eagle (Aquila heliaca) is one of the critically endangered
species in Serbia, with a small global population that will likely continue to decline. Despite
many biological and ecological studies on this species, little is known regarding the prevalence and incidence of infectious diseases in these birds. Here we present the pathological,
microbiological and toxicological findings in one imperial eagle.
PB  - European Society of Veterinary Pathology
C3  - Annual ESVP/ECVP Congress 2022, Athens, Greece, September 7 - 10, 2022
T1  - Pathological, Microbiological and Toxicological Findings in an Eastern Imperial Eagle
SP  - 57
EP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3361
ER  - 

@conference{
author = "Đurđević, Biljana and Pajić, Marko and Polaček, Vladimir and Knežević, Slobodan and Ratajac, Ratko and Mihaljev, Žarko and Aleksić-Kovačević, Sanja and Vučićević, Ivana",
year = "2022",
abstract = "The eastern imperial eagle (Aquila heliaca) is one of the critically endangered
species in Serbia, with a small global population that will likely continue to decline. Despite
many biological and ecological studies on this species, little is known regarding the prevalence and incidence of infectious diseases in these birds. Here we present the pathological,
microbiological and toxicological findings in one imperial eagle.",
publisher = "European Society of Veterinary Pathology",
journal = "Annual ESVP/ECVP Congress 2022, Athens, Greece, September 7 - 10, 2022",
title = "Pathological, Microbiological and Toxicological Findings in an Eastern Imperial Eagle",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3361"
}

Đurđević, B., Pajić, M., Polaček, V., Knežević, S., Ratajac, R., Mihaljev, Ž., Aleksić-Kovačević, S.,& Vučićević, I.. (2022). Pathological, Microbiological and Toxicological Findings in an Eastern Imperial Eagle. in Annual ESVP/ECVP Congress 2022, Athens, Greece, September 7 - 10, 2022
European Society of Veterinary Pathology., 57-57.
https://hdl.handle.net/21.15107/rcub_veterinar_3361

Đurđević B, Pajić M, Polaček V, Knežević S, Ratajac R, Mihaljev Ž, Aleksić-Kovačević S, Vučićević I. Pathological, Microbiological and Toxicological Findings in an Eastern Imperial Eagle. in Annual ESVP/ECVP Congress 2022, Athens, Greece, September 7 - 10, 2022. 2022;:57-57.
https://hdl.handle.net/21.15107/rcub_veterinar_3361 .

Đurđević, Biljana, Pajić, Marko, Polaček, Vladimir, Knežević, Slobodan, Ratajac, Ratko, Mihaljev, Žarko, Aleksić-Kovačević, Sanja, Vučićević, Ivana, "Pathological, Microbiological and Toxicological Findings in an Eastern Imperial Eagle" in Annual ESVP/ECVP Congress 2022, Athens, Greece, September 7 - 10, 2022 (2022):57-57,
https://hdl.handle.net/21.15107/rcub_veterinar_3361 .

TY  - JOUR
AU  - Đurđević, B.
AU  - Polaček, V.
AU  - Pajić, M.
AU  - Knežević, S.
AU  - Petrović, T.
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2865
PB  - Elsevier
T2  - Journal Of Comparative Pathology
T1  - New field outbreaks of highly pathogenic avian influenza h5n8 in mute swans in Serbia - pathological findings
VL  - 191
SP  - 53
EP  - 53
DO  - 10.1016/j.jcpa.2021.11.120
ER  - 

@article{
author = "Đurđević, B. and Polaček, V. and Pajić, M. and Knežević, S. and Petrović, T. and Vučićević, Ivana and Aleksić-Kovačević, Sanja",
year = "2022",
publisher = "Elsevier",
journal = "Journal Of Comparative Pathology",
title = "New field outbreaks of highly pathogenic avian influenza h5n8 in mute swans in Serbia - pathological findings",
volume = "191",
pages = "53-53",
doi = "10.1016/j.jcpa.2021.11.120"
}

Đurđević, B., Polaček, V., Pajić, M., Knežević, S., Petrović, T., Vučićević, I.,& Aleksić-Kovačević, S.. (2022). New field outbreaks of highly pathogenic avian influenza h5n8 in mute swans in Serbia - pathological findings. in Journal Of Comparative Pathology
Elsevier., 191, 53-53.
https://doi.org/10.1016/j.jcpa.2021.11.120

Đurđević B, Polaček V, Pajić M, Knežević S, Petrović T, Vučićević I, Aleksić-Kovačević S. New field outbreaks of highly pathogenic avian influenza h5n8 in mute swans in Serbia - pathological findings. in Journal Of Comparative Pathology. 2022;191:53-53.
doi:10.1016/j.jcpa.2021.11.120 .

Đurđević, B., Polaček, V., Pajić, M., Knežević, S., Petrović, T., Vučićević, Ivana, Aleksić-Kovačević, Sanja, "New field outbreaks of highly pathogenic avian influenza h5n8 in mute swans in Serbia - pathological findings" in Journal Of Comparative Pathology, 191 (2022):53-53,
https://doi.org/10.1016/j.jcpa.2021.11.120 . .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Jelisić, Stefan
AU  - Aleksić-Kovačević, Sanja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2868
AB  - Monitoring of scrapie in sheep and goats has been carried out for many years in Serbia.
Despite many years of monitoring, only one case of this disease has been reported in sheep,
but given that, there are numerous cases of the disease in some neighboring countries, it is
very important to determine the presence of resistant genes in the sheep population in Serbia.
Genetic susceptibility to scrapie is influenced mainly by the prion protein polymorphisms
of codons136, 154, and 171. The ARR allele is considered to provide very strong resistance
against classical scrapie and the VRQ genotype is the most susceptible. In order to examine
the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of
real-time polymerase chain reaction (qPCR) techniques for three codons. We analyzed blood
samples from 100 sheep using qPCR and the results showed that AA homozygous for the 136
codon were the most common. For codon 154 the most frequent genotype was RR and for
codon 171 the most frequent genotype was QQ.
C3  - 10th Iberian Congress on Prions, Vila Real, Portugal, 2022
T1  - Identification of the presence of a scrapie resistance gene in sheep on the territory of the Republic of Serbia
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2868
ER  - 

@conference{
author = "Vučićević, Ivana and Nešić, Slađan and Jelisić, Stefan and Aleksić-Kovačević, Sanja",
year = "2022",
abstract = "Monitoring of scrapie in sheep and goats has been carried out for many years in Serbia.
Despite many years of monitoring, only one case of this disease has been reported in sheep,
but given that, there are numerous cases of the disease in some neighboring countries, it is
very important to determine the presence of resistant genes in the sheep population in Serbia.
Genetic susceptibility to scrapie is influenced mainly by the prion protein polymorphisms
of codons136, 154, and 171. The ARR allele is considered to provide very strong resistance
against classical scrapie and the VRQ genotype is the most susceptible. In order to examine
the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of
real-time polymerase chain reaction (qPCR) techniques for three codons. We analyzed blood
samples from 100 sheep using qPCR and the results showed that AA homozygous for the 136
codon were the most common. For codon 154 the most frequent genotype was RR and for
codon 171 the most frequent genotype was QQ.",
journal = "10th Iberian Congress on Prions, Vila Real, Portugal, 2022",
title = "Identification of the presence of a scrapie resistance gene in sheep on the territory of the Republic of Serbia",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2868"
}

Vučićević, I., Nešić, S., Jelisić, S.,& Aleksić-Kovačević, S.. (2022). Identification of the presence of a scrapie resistance gene in sheep on the territory of the Republic of Serbia. in 10th Iberian Congress on Prions, Vila Real, Portugal, 2022.
https://hdl.handle.net/21.15107/rcub_veterinar_2868

Vučićević I, Nešić S, Jelisić S, Aleksić-Kovačević S. Identification of the presence of a scrapie resistance gene in sheep on the territory of the Republic of Serbia. in 10th Iberian Congress on Prions, Vila Real, Portugal, 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2868 .

Vučićević, Ivana, Nešić, Slađan, Jelisić, Stefan, Aleksić-Kovačević, Sanja, "Identification of the presence of a scrapie resistance gene in sheep on the territory of the Republic of Serbia" in 10th Iberian Congress on Prions, Vila Real, Portugal, 2022 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2868 .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Aničić, Milan
AU  - Ristoski, Trpe
AU  - Nikolić, Sonja
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2075
AB  - The ageing process is accompanied by numerous changes in the brain of dogs, such as accumulation of amyloid, fibrosis of blood vessel walls and meninges, accumulation of lipofuscin, and the presence of polyglucosan bodies (PGBs), satellitosis and neuronophagia. In this study, the presence of lipofuscin and PGBs in various parts of the brain in dogs of different sexes and ages was examined. For this purpose, brain samples were stained using haematoxylin eosin, modified Ziehl Neelsen and Periodic acid Schiff (PAS) methods. Lipofuscin was visualised by Ziehl Neelsen and PAS methods of specific staining on the same brain tissue segments. Lipofuscin had accumulated in 93% of old (more than 10 years old) dog brains, mostly in neurons of the medulla oblongata. The percentage of age-related lipofuscin pigment in other examined brain tissue segments was lower than in the medulla oblongata. There was a small difference in the percentage of lipofuscin-positive individuals between the two staining methods. The presence of PGBs was established by the PAS method for the vast majority (about 93%) of the old dogs (more than 10 years old), while PGBs were not detected in the group of young dogs (up to 5 years old). However, PGBs occurred in all examined segments of the dog’s brain tissues (for each of the tissue types, from 90% to 93% of dogs were positive for PGBs). The results obtained the oldest dogs (15 years old) harboured PGBs both extracellularly and intracellularly, while in other dogs, only extracellular PGBs were seen. Lipofuscin was accumulated mostly in large neurons of olivary nuclei of the medulla oblongata. PGBs were confirmed in all examined segments of the brain tissue of dogs more than 10 years old. This is one of the numerous indications that old dogs could be a very good animal model for studying the normal ageing process or neurodegenerative diseases.
AB  - Proces starenja prate brojne promene u mozgu pasa kao što su nagomilavanje amiloida, fibroza zida krvnih sudova i moždanih ovojnica, nakupljanje lipofuscina i prisustvo poliglukoznih tela (PGB), satelitoza i neuronofagija. U ovom radu ispitivano je prisustvo lipofuscina i PGB u različitim delovima centralnog nervnog sistema kod pasa različitog pola i starosti. Uzorci mozga obojeni su hematoksilin eozinom, modifikovanom Ziehl Neelsen metodom i perjodna kiselina-Schiff (PAS) metodom. Lipofuscin je modifikovanom Ziehl Neelsen i PAS metodom specifično dokazan u istim segmentima moždanog tkiva. Dobijeni rezultati pokazuju da je lipofuscin akumuliran uglavnom u neuronima produžene moždine kod 93% pasa. Zastupljenost pigmenta u ostalim segmentima mozga bio je niži u poređenju sa produženom moždinom. Korišćenjem obe metode bojenja, ustanovljena je mala razlika u procentu pozitivnih jedinki. Prisustvo PGB dokazano je PAS metodom kod velikog broja (oko 93%) pasa eksperimentalne grupe, dok u kontrolnoj grupi njihovo prisustvo nije ustanovljeno. U svim ispitivanim segmentima moždanog tkiva kod pasa iz eksperimentalne grupe, dokazana su PGB i to od 90% do 93%. Dobijeni rezultati ukazuju da su kod najstarijih pasa iz eksperimentalne grupe PGB bila lokalizovana i van ćelije i unutar nje, a kod drugih samo ekstracelularno. Lipofuscin je akumuliran uglavnom u velikim neuronima olivarnih jedara produžene moždine. PGB su dokazana u svim ispitivanim segmentima moždanog tkiva pasa starijih od 10 godina. Ovo je jedan od brojnih dokaza da stari psi predstavljaju dobar animalni model za proučavanje normalnog procesa starenja ili neurodegenerativnih bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old
T1  - Histohemijske karakteristike i distribucija lipofuscina i poliglukozanskih tela u mozgu pasa starijih od 10 godina
VL  - 75
IS  - 1
SP  - 57
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2075
ER  - 

@article{
author = "Nešić, Slađan and Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Aničić, Milan and Ristoski, Trpe and Nikolić, Sonja and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "The ageing process is accompanied by numerous changes in the brain of dogs, such as accumulation of amyloid, fibrosis of blood vessel walls and meninges, accumulation of lipofuscin, and the presence of polyglucosan bodies (PGBs), satellitosis and neuronophagia. In this study, the presence of lipofuscin and PGBs in various parts of the brain in dogs of different sexes and ages was examined. For this purpose, brain samples were stained using haematoxylin eosin, modified Ziehl Neelsen and Periodic acid Schiff (PAS) methods. Lipofuscin was visualised by Ziehl Neelsen and PAS methods of specific staining on the same brain tissue segments. Lipofuscin had accumulated in 93% of old (more than 10 years old) dog brains, mostly in neurons of the medulla oblongata. The percentage of age-related lipofuscin pigment in other examined brain tissue segments was lower than in the medulla oblongata. There was a small difference in the percentage of lipofuscin-positive individuals between the two staining methods. The presence of PGBs was established by the PAS method for the vast majority (about 93%) of the old dogs (more than 10 years old), while PGBs were not detected in the group of young dogs (up to 5 years old). However, PGBs occurred in all examined segments of the dog’s brain tissues (for each of the tissue types, from 90% to 93% of dogs were positive for PGBs). The results obtained the oldest dogs (15 years old) harboured PGBs both extracellularly and intracellularly, while in other dogs, only extracellular PGBs were seen. Lipofuscin was accumulated mostly in large neurons of olivary nuclei of the medulla oblongata. PGBs were confirmed in all examined segments of the brain tissue of dogs more than 10 years old. This is one of the numerous indications that old dogs could be a very good animal model for studying the normal ageing process or neurodegenerative diseases., Proces starenja prate brojne promene u mozgu pasa kao što su nagomilavanje amiloida, fibroza zida krvnih sudova i moždanih ovojnica, nakupljanje lipofuscina i prisustvo poliglukoznih tela (PGB), satelitoza i neuronofagija. U ovom radu ispitivano je prisustvo lipofuscina i PGB u različitim delovima centralnog nervnog sistema kod pasa različitog pola i starosti. Uzorci mozga obojeni su hematoksilin eozinom, modifikovanom Ziehl Neelsen metodom i perjodna kiselina-Schiff (PAS) metodom. Lipofuscin je modifikovanom Ziehl Neelsen i PAS metodom specifično dokazan u istim segmentima moždanog tkiva. Dobijeni rezultati pokazuju da je lipofuscin akumuliran uglavnom u neuronima produžene moždine kod 93% pasa. Zastupljenost pigmenta u ostalim segmentima mozga bio je niži u poređenju sa produženom moždinom. Korišćenjem obe metode bojenja, ustanovljena je mala razlika u procentu pozitivnih jedinki. Prisustvo PGB dokazano je PAS metodom kod velikog broja (oko 93%) pasa eksperimentalne grupe, dok u kontrolnoj grupi njihovo prisustvo nije ustanovljeno. U svim ispitivanim segmentima moždanog tkiva kod pasa iz eksperimentalne grupe, dokazana su PGB i to od 90% do 93%. Dobijeni rezultati ukazuju da su kod najstarijih pasa iz eksperimentalne grupe PGB bila lokalizovana i van ćelije i unutar nje, a kod drugih samo ekstracelularno. Lipofuscin je akumuliran uglavnom u velikim neuronima olivarnih jedara produžene moždine. PGB su dokazana u svim ispitivanim segmentima moždanog tkiva pasa starijih od 10 godina. Ovo je jedan od brojnih dokaza da stari psi predstavljaju dobar animalni model za proučavanje normalnog procesa starenja ili neurodegenerativnih bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old, Histohemijske karakteristike i distribucija lipofuscina i poliglukozanskih tela u mozgu pasa starijih od 10 godina",
volume = "75",
number = "1",
pages = "57-68",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2075"
}

Nešić, S., Vučićević, I., Marinković, D., Kukolj, V., Aničić, M., Ristoski, T., Nikolić, S.,& Aleksić-Kovačević, S.. (2021). Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 57-68.
https://hdl.handle.net/21.15107/rcub_veterinar_2075

Nešić S, Vučićević I, Marinković D, Kukolj V, Aničić M, Ristoski T, Nikolić S, Aleksić-Kovačević S. Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old. in Veterinarski Glasnik. 2021;75(1):57-68.
https://hdl.handle.net/21.15107/rcub_veterinar_2075 .

Nešić, Slađan, Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Aničić, Milan, Ristoski, Trpe, Nikolić, Sonja, Aleksić-Kovačević, Sanja, "Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old" in Veterinarski Glasnik, 75, no. 1 (2021):57-68,
https://hdl.handle.net/21.15107/rcub_veterinar_2075 .

TY  - JOUR
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Marinković, Darko
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2326
AB  - Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions
in this animal species. The aim of this study was to identify the most common types
of canine cutaneous lesions, to determine the absolute and relative frequency of
each type of cutaneous lesion, anatomical locations, mean age, as well as gender and
breed distribution. The examination included all samples of cutaneous lesions in dogs
obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of
the Department of Pathology at the Faculty of Veterinary Medicine, University of
Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a
total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%)
were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448
(18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous
tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma
(7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%),
hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common
non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic
lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis
(7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results
substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic
lesions in dogs, and provide updated information on their frequency, animal
age, anatomic location and breed distributions.
AB  - Kožne lezije kod pasa, naročito tumori, spadaju među najčešće lezije kod ove životinjske
vrste. Cilj ovog istraživanja bio je da se identifikuju najčešći tipovi kožnih lezija
kod pasa, kao i da se utvrde apsolutna i relativna učestalost svake vrste kožnih lezija,
anatomska lokacija, prosečna starost, kao i rasna i polna distribucija. Ispitivanjem su
obuhvaćeni svi uzorci lezija kože kod pasa koji su dobijeni hirurškom biopsijom u
veterinarskim ambulantama i pregledani u laboratoriji Katedre za patologiju Fakulteta
veterinarske medicine Univerziteta u Beogradu u periodu od 1. januara 2011. do 1.
jula 2021. godine. U ovom periodu od 126 meseci, pregledana su ukupno 2432 uzorka
kožnih lezija, od čega su 1984 (81,58%) bili tumori (1037/1984, 52,27% benigni i
947/1984, 47,73% maligni) i 448 (18,42%) neneoplastične lezije kože. Najčešći tumori
kože bili su: mastocitom (17,34% svih tumora), histiocitom (9,78%), papilom (7,91%),
lipom (7,81%), planocelularni karcinom (7,36%), trihoblastom (4,44%), adenom hepatoidne
žlezde (4,39%) i maligni melanom (4,18%). Najčešće neneoplastične lezije
kože bile su: folikularne ciste (35,04% svih neneoplastičnih lezija), hronični piogranulomatozni
dermatitis (23,88%), limfocitni dermatitis (7,37%), hiperkeratoza (4,24%)
i granulomatozni dermatitis (3,79%). Naši rezultati u velikoj meri potvrđuju ranije
objavljene podatke o neoplastičnim i neneoplastičnim lezijama kože kod pasa i pružaju
nove ažurirane informacije o njihovoj učestalosti, starosti životinje, anatomskoj lokaciji
i rasnoj i polnoj distribuciji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria-Beograd
T1  - Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021)
T1  - Prevalenca i distribucija neoplastičnih i neneoplastičnih lezija kože kod pasa u Srbiji: Retrospektivna studija 2432 slučaja (2011 - sredina 2021)
VL  - 71
IS  - 4
SP  - 403
EP  - 416
DO  - 10.2478/acve-2021-0034
ER  - 

@article{
author = "Kukolj, Vladimir and Nešić, Slađan and Marinković, Darko and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions
in this animal species. The aim of this study was to identify the most common types
of canine cutaneous lesions, to determine the absolute and relative frequency of
each type of cutaneous lesion, anatomical locations, mean age, as well as gender and
breed distribution. The examination included all samples of cutaneous lesions in dogs
obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of
the Department of Pathology at the Faculty of Veterinary Medicine, University of
Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a
total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%)
were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448
(18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous
tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma
(7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%),
hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common
non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic
lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis
(7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results
substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic
lesions in dogs, and provide updated information on their frequency, animal
age, anatomic location and breed distributions., Kožne lezije kod pasa, naročito tumori, spadaju među najčešće lezije kod ove životinjske
vrste. Cilj ovog istraživanja bio je da se identifikuju najčešći tipovi kožnih lezija
kod pasa, kao i da se utvrde apsolutna i relativna učestalost svake vrste kožnih lezija,
anatomska lokacija, prosečna starost, kao i rasna i polna distribucija. Ispitivanjem su
obuhvaćeni svi uzorci lezija kože kod pasa koji su dobijeni hirurškom biopsijom u
veterinarskim ambulantama i pregledani u laboratoriji Katedre za patologiju Fakulteta
veterinarske medicine Univerziteta u Beogradu u periodu od 1. januara 2011. do 1.
jula 2021. godine. U ovom periodu od 126 meseci, pregledana su ukupno 2432 uzorka
kožnih lezija, od čega su 1984 (81,58%) bili tumori (1037/1984, 52,27% benigni i
947/1984, 47,73% maligni) i 448 (18,42%) neneoplastične lezije kože. Najčešći tumori
kože bili su: mastocitom (17,34% svih tumora), histiocitom (9,78%), papilom (7,91%),
lipom (7,81%), planocelularni karcinom (7,36%), trihoblastom (4,44%), adenom hepatoidne
žlezde (4,39%) i maligni melanom (4,18%). Najčešće neneoplastične lezije
kože bile su: folikularne ciste (35,04% svih neneoplastičnih lezija), hronični piogranulomatozni
dermatitis (23,88%), limfocitni dermatitis (7,37%), hiperkeratoza (4,24%)
i granulomatozni dermatitis (3,79%). Naši rezultati u velikoj meri potvrđuju ranije
objavljene podatke o neoplastičnim i neneoplastičnim lezijama kože kod pasa i pružaju
nove ažurirane informacije o njihovoj učestalosti, starosti životinje, anatomskoj lokaciji
i rasnoj i polnoj distribuciji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria-Beograd",
title = "Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021), Prevalenca i distribucija neoplastičnih i neneoplastičnih lezija kože kod pasa u Srbiji: Retrospektivna studija 2432 slučaja (2011 - sredina 2021)",
volume = "71",
number = "4",
pages = "403-416",
doi = "10.2478/acve-2021-0034"
}

Kukolj, V., Nešić, S., Marinković, D.,& Aleksić-Kovačević, S.. (2021). Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021). in Acta veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(4), 403-416.
https://doi.org/10.2478/acve-2021-0034

Kukolj V, Nešić S, Marinković D, Aleksić-Kovačević S. Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021). in Acta veterinaria-Beograd. 2021;71(4):403-416.
doi:10.2478/acve-2021-0034 .

Kukolj, Vladimir, Nešić, Slađan, Marinković, Darko, Aleksić-Kovačević, Sanja, "Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021)" in Acta veterinaria-Beograd, 71, no. 4 (2021):403-416,
https://doi.org/10.2478/acve-2021-0034 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Aleksić-Kovačević, Sanja
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
AU  - Maksimović Zorić, Jelena
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Knežević, Đorđe
AU  - Spalević, Ljiljana
AU  - Kukolj, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2784
AB  - Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.
PB  - Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)
T2  - Veterinaria Italiana
T1  - Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
VL  - 54
IS  - 7
SP  - 287
EP  - 295
DO  - 10.12834/VetIt.1813.9553.3
ER  - 

@article{
author = "Kureljušić, Branislav and Aleksić-Kovačević, Sanja and Savić, Božidar and Prodanović, Radiša and Jezdimirović, Nemanja and Milićević, Vesna and Maksimović Zorić, Jelena and Kureljušić, Jasna and Žutić, Jadranka and Knežević, Đorđe and Spalević, Ljiljana and Kukolj, Vladimir",
year = "2021",
abstract = "Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.",
publisher = "Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)",
journal = "Veterinaria Italiana",
title = "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus",
volume = "54",
number = "7",
pages = "287-295",
doi = "10.12834/VetIt.1813.9553.3"
}

Kureljušić, B., Aleksić-Kovačević, S., Savić, B., Prodanović, R., Jezdimirović, N., Milićević, V., Maksimović Zorić, J., Kureljušić, J., Žutić, J., Knežević, Đ., Spalević, L.,& Kukolj, V.. (2021). Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)., 54(7), 287-295.
https://doi.org/10.12834/VetIt.1813.9553.3

Kureljušić B, Aleksić-Kovačević S, Savić B, Prodanović R, Jezdimirović N, Milićević V, Maksimović Zorić J, Kureljušić J, Žutić J, Knežević Đ, Spalević L, Kukolj V. Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana. 2021;54(7):287-295.
doi:10.12834/VetIt.1813.9553.3 .

Kureljušić, Branislav, Aleksić-Kovačević, Sanja, Savić, Božidar, Prodanović, Radiša, Jezdimirović, Nemanja, Milićević, Vesna, Maksimović Zorić, Jelena, Kureljušić, Jasna, Žutić, Jadranka, Knežević, Đorđe, Spalević, Ljiljana, Kukolj, Vladimir, "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus" in Veterinaria Italiana, 54, no. 7 (2021):287-295,
https://doi.org/10.12834/VetIt.1813.9553.3 . .

TY  - CONF
AU  - Jelesijević, Tomislav
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2007
AB  - U novembru 2002. godine, u kineskoj provinciji Guandong poče- la je epidemija, životno ugrožavajuće respiratorne bolesti nepoznate etiologije. Bolest se odlikovala teškim akutnim respiratornim sindro- mom i skraćeno se naziva SARS, a pred kraj ove pandemije, otkrive- no je da je prouzrokovana novim korona virusom (SARS-CoV). Iako su azijske cibetke (Pagumalarvata) direktno prenosile prepandemij- ski soj virusa na ljude, stvarni izvori virusa bile su različite vrste slepih miševa. Bolest se iz Kine proširila na 30 zemalja, dijagnostikovana je kod 8 000 ljudi i prouzrokovala je stopu smrtnosti od 10 posto. Slično prvoj, druga pandemija SARS-a je počela u kineskom gradu Vuhanu 2019. Godine i bila je povezana sa najmanje dve vrste sisara: pango- linima i slepim miševima. SARS-CoV 2, kao i bolest koja iz njega proi- stiče - COVID 19, su ovoga puta zarazili desetine miliona ljudi, godinu dana od izbijanja pandemije. COVID 19 je prouzrokovao stopu smrt- nosti od 2,4% i značajno je uticao na zdravstvene, obrazovne i eko- nomske sisteme i živote ljudi. Pokrenuta su brojna komparativna ispiti- vanja na animalnim modelima i uočene su razlike, ali i brojne sličnosti koronavirusnih infekcija mačaka i ljudi. Ponovljeni način prenošrnja i procena postojanja nekoliko hiljada korona virusa slepih miševa su- gerišu da prošla, sadašnja i buduća pandemija SARS-a, ne treba da budu iznenađenje.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
T1  - Od SARS-a do Kovid-a 19: od delimično istražene bolesti prošlosti do pandemije sadašnjosti
EP  - 65
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2007
ER  - 

@conference{
author = "Jelesijević, Tomislav and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "U novembru 2002. godine, u kineskoj provinciji Guandong poče- la je epidemija, životno ugrožavajuće respiratorne bolesti nepoznate etiologije. Bolest se odlikovala teškim akutnim respiratornim sindro- mom i skraćeno se naziva SARS, a pred kraj ove pandemije, otkrive- no je da je prouzrokovana novim korona virusom (SARS-CoV). Iako su azijske cibetke (Pagumalarvata) direktno prenosile prepandemij- ski soj virusa na ljude, stvarni izvori virusa bile su različite vrste slepih miševa. Bolest se iz Kine proširila na 30 zemalja, dijagnostikovana je kod 8 000 ljudi i prouzrokovala je stopu smrtnosti od 10 posto. Slično prvoj, druga pandemija SARS-a je počela u kineskom gradu Vuhanu 2019. Godine i bila je povezana sa najmanje dve vrste sisara: pango- linima i slepim miševima. SARS-CoV 2, kao i bolest koja iz njega proi- stiče - COVID 19, su ovoga puta zarazili desetine miliona ljudi, godinu dana od izbijanja pandemije. COVID 19 je prouzrokovao stopu smrt- nosti od 2,4% i značajno je uticao na zdravstvene, obrazovne i eko- nomske sisteme i živote ljudi. Pokrenuta su brojna komparativna ispiti- vanja na animalnim modelima i uočene su razlike, ali i brojne sličnosti koronavirusnih infekcija mačaka i ljudi. Ponovljeni način prenošrnja i procena postojanja nekoliko hiljada korona virusa slepih miševa su- gerišu da prošla, sadašnja i buduća pandemija SARS-a, ne treba da budu iznenađenje.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021",
title = "Od SARS-a do Kovid-a 19: od delimično istražene bolesti prošlosti do pandemije sadašnjosti",
pages = "65-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2007"
}

Jelesijević, T.,& Aleksić-Kovačević, S.. (2021). Od SARS-a do Kovid-a 19: od delimično istražene bolesti prošlosti do pandemije sadašnjosti. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila..
https://hdl.handle.net/21.15107/rcub_veterinar_2007

Jelesijević T, Aleksić-Kovačević S. Od SARS-a do Kovid-a 19: od delimično istražene bolesti prošlosti do pandemije sadašnjosti. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021. 2021;:null-65.
https://hdl.handle.net/21.15107/rcub_veterinar_2007 .

Jelesijević, Tomislav, Aleksić-Kovačević, Sanja, "Od SARS-a do Kovid-a 19: od delimično istražene bolesti prošlosti do pandemije sadašnjosti" in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_2007 .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Đurđević, Biljana
AU  - Nešić, Slađan
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2005
AB  - Usled jesenje migracije ptica ka teritoriji Evrope, postoji kontinui- rani rizik od pojave novih slučajeva visoko patogene avijarne influence (HPAI), kako kod divljih, tako i kod domaćih ptica. Tokom jeseni 2020. godine dijagnostikovano je preko 300 slučajeva HPAI-A(H5) kod divljih ptica i živine u zemljama EU. Broj žarišta HPAI se drastično povećava i dok je proteklih godina glavni uzročnik epidemije bio A(H5N8) podtip virusa, sada je zastupljeno nekoliko podtipova: A(H5N8), A(H5N5) i A(H5N1). Kod infekcije HPAI virusima, tip promena koje se sreću na obdukciji, kao i njihova lokalizacija mogu se donekle razlikovati u zavi- snosti od podtipa virusa i vrste ptica. Promene se javljaju u vidu nekro- za, edema i hemoragija, a najčešće su locirane na plućima, srcu, jetri, slezini, bubrezima, mozgu i koži. Obdukcija ptica sumnjivih na HPAI se obavlja u prostorijama sa biosigurnosnim nivoom 2, noseći, pored standardne zaštitne opreme još i maske N95. Obdukcija se vrši prema standardnim protokolima za obdukciju ptica koji uključuju spoljašnji i unutrašnji pregled leša. Prilikom obdukcije, potrebno je uzeti uzorke tkiva promenjenih organa i ona, koja su namenjena za patohistološ- ku analizu, fiksirati u 10% puferizovanom formalinu najmanje 48 ča- sova. Uzorci ne smeju biti deblji od 0,5 cm, kako bi fiksativ magao da prodre kroz tkivo. Obdukovane leševe ptica treba neškodljivo ukloniti, kao materijal kategorije 1.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
T1  - Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku
SP  - 149
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2005
ER  - 

@conference{
author = "Vučićević, Ivana and Đurđević, Biljana and Nešić, Slađan and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "Usled jesenje migracije ptica ka teritoriji Evrope, postoji kontinui- rani rizik od pojave novih slučajeva visoko patogene avijarne influence (HPAI), kako kod divljih, tako i kod domaćih ptica. Tokom jeseni 2020. godine dijagnostikovano je preko 300 slučajeva HPAI-A(H5) kod divljih ptica i živine u zemljama EU. Broj žarišta HPAI se drastično povećava i dok je proteklih godina glavni uzročnik epidemije bio A(H5N8) podtip virusa, sada je zastupljeno nekoliko podtipova: A(H5N8), A(H5N5) i A(H5N1). Kod infekcije HPAI virusima, tip promena koje se sreću na obdukciji, kao i njihova lokalizacija mogu se donekle razlikovati u zavi- snosti od podtipa virusa i vrste ptica. Promene se javljaju u vidu nekro- za, edema i hemoragija, a najčešće su locirane na plućima, srcu, jetri, slezini, bubrezima, mozgu i koži. Obdukcija ptica sumnjivih na HPAI se obavlja u prostorijama sa biosigurnosnim nivoom 2, noseći, pored standardne zaštitne opreme još i maske N95. Obdukcija se vrši prema standardnim protokolima za obdukciju ptica koji uključuju spoljašnji i unutrašnji pregled leša. Prilikom obdukcije, potrebno je uzeti uzorke tkiva promenjenih organa i ona, koja su namenjena za patohistološ- ku analizu, fiksirati u 10% puferizovanom formalinu najmanje 48 ča- sova. Uzorci ne smeju biti deblji od 0,5 cm, kako bi fiksativ magao da prodre kroz tkivo. Obdukovane leševe ptica treba neškodljivo ukloniti, kao materijal kategorije 1.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021",
title = "Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku",
pages = "149-158",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2005"
}

Vučićević, I., Đurđević, B., Nešić, S.,& Aleksić-Kovačević, S.. (2021). Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 149-158.
https://hdl.handle.net/21.15107/rcub_veterinar_2005

Vučićević I, Đurđević B, Nešić S, Aleksić-Kovačević S. Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021. 2021;:149-158.
https://hdl.handle.net/21.15107/rcub_veterinar_2005 .

Vučićević, Ivana, Đurđević, Biljana, Nešić, Slađan, Aleksić-Kovačević, Sanja, "Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku" in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021 (2021):149-158,
https://hdl.handle.net/21.15107/rcub_veterinar_2005 .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Jelisić, Stefan
AU  - Aleksić-Kovačević, Sanja
AU  - Aničić, Milan
AU  - Vučićević, Ivana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2191
AB  - Scrapie is an infectious neurodegenerative disease affecting the central nervous system of sheep and goats that belongs to transmissible spongiform encephalopathies. The disease is caused by the accumulation of proteinase-resistant isoform of the prion protein. The sheep predisposition to scrapie is associated with polymorphisms of the PrP gene. Genetic susceptibility to scrapie is mainly related to codons 136, 154, and 171. ARR sheep are strongly scrapie resistant and VRQ genotype is the most susceptible. Many countries have scrapie eradication programs based on using rams with resistant genotype. The eradication program has not yet been implemented in the Republic of Serbia. To examine the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of real-time polymerase chain reaction (qPCR) technique for three codons. Blood samples from 100 sheep were analyzed by qPCR and the majority of the examined sheep were AA homozygous for the 136 codon. For codon 154 the most frequent genotype was RR and for codon 171 the most frequent genotype was QQ.
AB  - Ovčija svrabež je infektivno neurodegenerativno oboljenje iz grupe transmisivnih encefalopatija koje uzrokuje promene u moždanom tkivu ovaca i koza. Oboljenje je uzrokovano akumulacijom proteinaza-rezistentne izoforme prion proteina. Osetljivost ovaca na ovo oboljenje uslovljena je polimorfi zmom PrP gena. Genetska otpornost na ovčiju svrabež prevashodno je uslovljena kodonima 136, 154 i 171. Najotpornije su ovce sa ARR genotipom, dok se ovce sa VRQ genotipom smatraju najosetljivijim. Mnoge zemlje su sprovele programe eradikacije ovčije svrabeži bazirane na korišćenju priplodnih ovnova koji imaju rezistentan genotip. Ovakav program još nije sproveden u Republici Srbiji. U cilju ispitivanja genetskog statusa ovaca u Srbiji u pogledu otpornosti na ovčiju svrabež, optimizovali smo TaqMan probu, kvantitativne lančane reakcije polimeraze (qPCR) tehnike za sva tri kodona. Ispitivanjem uzoroka krvi od 100 ovaca primenom RT-PCR ustanovljeno je da je većina ovaca AA homozigot za kodon 136. Za kodon 154 najčešći genotip je RR, a za kodon 171 genotip QQ.
T2  - Acta Veterinaria-Beograd
T1  - Scrapie resistance gene identification using optimized TaqMan test qPCR method in sheep on the territory of the Republic of Serbia
T1  - Identifikacija optimizovanom taqman probom qpcr metodom gena rezistencije na scrapie kod ovaca na teritoriji Republike Srbije
VL  - 71
IS  - 2
SP  - 189
EP  - 197
DO  - 10.2478/acve-2021-0016
ER  - 

@article{
author = "Nešić, Slađan and Jelisić, Stefan and Aleksić-Kovačević, Sanja and Aničić, Milan and Vučićević, Ivana",
year = "2021",
abstract = "Scrapie is an infectious neurodegenerative disease affecting the central nervous system of sheep and goats that belongs to transmissible spongiform encephalopathies. The disease is caused by the accumulation of proteinase-resistant isoform of the prion protein. The sheep predisposition to scrapie is associated with polymorphisms of the PrP gene. Genetic susceptibility to scrapie is mainly related to codons 136, 154, and 171. ARR sheep are strongly scrapie resistant and VRQ genotype is the most susceptible. Many countries have scrapie eradication programs based on using rams with resistant genotype. The eradication program has not yet been implemented in the Republic of Serbia. To examine the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of real-time polymerase chain reaction (qPCR) technique for three codons. Blood samples from 100 sheep were analyzed by qPCR and the majority of the examined sheep were AA homozygous for the 136 codon. For codon 154 the most frequent genotype was RR and for codon 171 the most frequent genotype was QQ., Ovčija svrabež je infektivno neurodegenerativno oboljenje iz grupe transmisivnih encefalopatija koje uzrokuje promene u moždanom tkivu ovaca i koza. Oboljenje je uzrokovano akumulacijom proteinaza-rezistentne izoforme prion proteina. Osetljivost ovaca na ovo oboljenje uslovljena je polimorfi zmom PrP gena. Genetska otpornost na ovčiju svrabež prevashodno je uslovljena kodonima 136, 154 i 171. Najotpornije su ovce sa ARR genotipom, dok se ovce sa VRQ genotipom smatraju najosetljivijim. Mnoge zemlje su sprovele programe eradikacije ovčije svrabeži bazirane na korišćenju priplodnih ovnova koji imaju rezistentan genotip. Ovakav program još nije sproveden u Republici Srbiji. U cilju ispitivanja genetskog statusa ovaca u Srbiji u pogledu otpornosti na ovčiju svrabež, optimizovali smo TaqMan probu, kvantitativne lančane reakcije polimeraze (qPCR) tehnike za sva tri kodona. Ispitivanjem uzoroka krvi od 100 ovaca primenom RT-PCR ustanovljeno je da je većina ovaca AA homozigot za kodon 136. Za kodon 154 najčešći genotip je RR, a za kodon 171 genotip QQ.",
journal = "Acta Veterinaria-Beograd",
title = "Scrapie resistance gene identification using optimized TaqMan test qPCR method in sheep on the territory of the Republic of Serbia, Identifikacija optimizovanom taqman probom qpcr metodom gena rezistencije na scrapie kod ovaca na teritoriji Republike Srbije",
volume = "71",
number = "2",
pages = "189-197",
doi = "10.2478/acve-2021-0016"
}

Nešić, S., Jelisić, S., Aleksić-Kovačević, S., Aničić, M.,& Vučićević, I.. (2021). Scrapie resistance gene identification using optimized TaqMan test qPCR method in sheep on the territory of the Republic of Serbia. in Acta Veterinaria-Beograd, 71(2), 189-197.
https://doi.org/10.2478/acve-2021-0016

Nešić S, Jelisić S, Aleksić-Kovačević S, Aničić M, Vučićević I. Scrapie resistance gene identification using optimized TaqMan test qPCR method in sheep on the territory of the Republic of Serbia. in Acta Veterinaria-Beograd. 2021;71(2):189-197.
doi:10.2478/acve-2021-0016 .

Nešić, Slađan, Jelisić, Stefan, Aleksić-Kovačević, Sanja, Aničić, Milan, Vučićević, Ivana, "Scrapie resistance gene identification using optimized TaqMan test qPCR method in sheep on the territory of the Republic of Serbia" in Acta Veterinaria-Beograd, 71, no. 2 (2021):189-197,
https://doi.org/10.2478/acve-2021-0016 . .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Andrić, Nenad
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1876
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Comparative Pathology
T1  - Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier
VL  - 174
SP  - 168
DO  - 10.1016/j.jcpa.2019.10.091
ER  - 

@article{
author = "Nešić, Slađan and Andrić, Nenad and Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2020",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier",
volume = "174",
pages = "168",
doi = "10.1016/j.jcpa.2019.10.091"
}

Nešić, S., Andrić, N., Vučićević, I., Marinković, D., Kukolj, V., Aničić, M.,& Aleksić-Kovačević, S.. (2020). Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 174, 168.
https://doi.org/10.1016/j.jcpa.2019.10.091

Nešić S, Andrić N, Vučićević I, Marinković D, Kukolj V, Aničić M, Aleksić-Kovačević S. Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier. in Journal of Comparative Pathology. 2020;174:168.
doi:10.1016/j.jcpa.2019.10.091 .

Nešić, Slađan, Andrić, Nenad, Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Aničić, Milan, Aleksić-Kovačević, Sanja, "Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier" in Journal of Comparative Pathology, 174 (2020):168,
https://doi.org/10.1016/j.jcpa.2019.10.091 . .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2908
AB  - Transmisivne spongiformne encefalopatije (TSE) su neurodegenerativna oboljenja životinja i ljudi koja se karakterišu nakupljanjem izmenjenog oblika prionskog proteina (PrPres) u mozgu. Asimptomatski
period može trajati i nekoliko godina, ali je bolest uvek sa smrtnim ishodom. Monitoring TSE obuhvata ispitivanje prisustva PrPres kod goveda, a zatim i kod ovaca i koza, kod kojih uzrokuje oboljenje pod nazivom “ovčija svrabež”. U novije vreme se, u većini zemalja EU, naročita pažnja poklanja prisustvu PrPres kod jelenske divljači i pojavi hronične slabosti jelena. Spongiformna encefalopatija goveda i “ovčija
svrabež” se javljaju u klasičnoj i atipičnoj formi, pri čemu se smatra da
atipična forma nastaje spontano i da nije kontagiozna. Na osnovu OIE
podele, razlikuju se dve kategorije statusa u pogledu prisustva bovine
spongiformne encefalopatije (BSE). Od 2019. godine, Republika Srbija je stekla status zemlje sa zanemarljivim rizikom. Radi održavanja
ovog statusa zemlje, potrebno je od svih propisanih vrsta i kategorija životinja vršiti pravilno uzorkovanje i ispitivanje tkiva. Za analizu se
uzorkuje deo produžene moždine u nivou obex-a, odnosno ceo mozak kod klinički sumnjivih slučajeva. Uzorkovanje produžene moždine, bez otvaranja lobanje, obavlja se upotrebom instrumenta u obliku kašike sa oštrim ivicama. Greške prilikom uzorkovanja, pakovanja,
slanja i obeležavanja uzorkovanog materijala mogu ugroziti verodostojnost rezultata laboratorijske analize.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLI Seminar za inovacije znanja veterinara, Beograd, 2020
T1  - Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika
SP  - 77
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2908
ER  - 

@conference{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "Transmisivne spongiformne encefalopatije (TSE) su neurodegenerativna oboljenja životinja i ljudi koja se karakterišu nakupljanjem izmenjenog oblika prionskog proteina (PrPres) u mozgu. Asimptomatski
period može trajati i nekoliko godina, ali je bolest uvek sa smrtnim ishodom. Monitoring TSE obuhvata ispitivanje prisustva PrPres kod goveda, a zatim i kod ovaca i koza, kod kojih uzrokuje oboljenje pod nazivom “ovčija svrabež”. U novije vreme se, u većini zemalja EU, naročita pažnja poklanja prisustvu PrPres kod jelenske divljači i pojavi hronične slabosti jelena. Spongiformna encefalopatija goveda i “ovčija
svrabež” se javljaju u klasičnoj i atipičnoj formi, pri čemu se smatra da
atipična forma nastaje spontano i da nije kontagiozna. Na osnovu OIE
podele, razlikuju se dve kategorije statusa u pogledu prisustva bovine
spongiformne encefalopatije (BSE). Od 2019. godine, Republika Srbija je stekla status zemlje sa zanemarljivim rizikom. Radi održavanja
ovog statusa zemlje, potrebno je od svih propisanih vrsta i kategorija životinja vršiti pravilno uzorkovanje i ispitivanje tkiva. Za analizu se
uzorkuje deo produžene moždine u nivou obex-a, odnosno ceo mozak kod klinički sumnjivih slučajeva. Uzorkovanje produžene moždine, bez otvaranja lobanje, obavlja se upotrebom instrumenta u obliku kašike sa oštrim ivicama. Greške prilikom uzorkovanja, pakovanja,
slanja i obeležavanja uzorkovanog materijala mogu ugroziti verodostojnost rezultata laboratorijske analize.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLI Seminar za inovacije znanja veterinara, Beograd, 2020",
title = "Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika",
pages = "77-86",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2908"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2020). Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 77-86.
https://hdl.handle.net/21.15107/rcub_veterinar_2908

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Aleksić-Kovačević S. Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020. 2020;:77-86.
https://hdl.handle.net/21.15107/rcub_veterinar_2908 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika" in XLI Seminar za inovacije znanja veterinara, Beograd, 2020 (2020):77-86,
https://hdl.handle.net/21.15107/rcub_veterinar_2908 .

TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Vučićević, Ivana
AU  - Polgek, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1819
AB  - Swine respiratory diseases represent one of the most frequent health issues in pig production worldwide. Despite the great progress that has been made in the field of diagnostics, control and prophylaxis, respiratory diseases still remain the most challenging health problem in modern commercial pig production. The list of infectious agents that cause respiratory diseases in swine is extensive and includes both, bacterial and viral pathogens. In Serbia, more than fifteen years after the introduction of modern vaccines, the list of bacterial pathogens related to swine respiratory infections still include Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida. On the other hand, most commonly involved viral pathogens are Porcine Reproductive and Respiratory Syndrome Virus, Swine influenza virus, Porcine circovirus type 2 and Pseudorabies virus. The morphological features of pneumonia where several agents are involved, depend on the predominant etiological agent. Expanding knowledge of the main pathogens associated with swine respiratory diseases and the effects of their interactions on the disease outcome is important for further investigations of lung diseases and implementation of control strategies in commercial pig populations in Serbia. This review discusses the latest findings on swine respiratory disease and current trends in Serbian pig production.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Current swine respiratory diseases morphology in intensive swine production in Serbia
VL  - 70
IS  - 1
SP  - 1
EP  - 36
DO  - 10.2478/acve-2020-0001
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Vučićević, Ivana and Polgek, Vladimir and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "Swine respiratory diseases represent one of the most frequent health issues in pig production worldwide. Despite the great progress that has been made in the field of diagnostics, control and prophylaxis, respiratory diseases still remain the most challenging health problem in modern commercial pig production. The list of infectious agents that cause respiratory diseases in swine is extensive and includes both, bacterial and viral pathogens. In Serbia, more than fifteen years after the introduction of modern vaccines, the list of bacterial pathogens related to swine respiratory infections still include Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida. On the other hand, most commonly involved viral pathogens are Porcine Reproductive and Respiratory Syndrome Virus, Swine influenza virus, Porcine circovirus type 2 and Pseudorabies virus. The morphological features of pneumonia where several agents are involved, depend on the predominant etiological agent. Expanding knowledge of the main pathogens associated with swine respiratory diseases and the effects of their interactions on the disease outcome is important for further investigations of lung diseases and implementation of control strategies in commercial pig populations in Serbia. This review discusses the latest findings on swine respiratory disease and current trends in Serbian pig production.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Current swine respiratory diseases morphology in intensive swine production in Serbia",
volume = "70",
number = "1",
pages = "1-36",
doi = "10.2478/acve-2020-0001"
}

Prodanov-Radulović, J., Vučićević, I., Polgek, V.,& Aleksić-Kovačević, S.. (2020). Current swine respiratory diseases morphology in intensive swine production in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 1-36.
https://doi.org/10.2478/acve-2020-0001

Prodanov-Radulović J, Vučićević I, Polgek V, Aleksić-Kovačević S. Current swine respiratory diseases morphology in intensive swine production in Serbia. in Acta Veterinaria-Beograd. 2020;70(1):1-36.
doi:10.2478/acve-2020-0001 .

Prodanov-Radulović, Jasna, Vučićević, Ivana, Polgek, Vladimir, Aleksić-Kovačević, Sanja, "Current swine respiratory diseases morphology in intensive swine production in Serbia" in Acta Veterinaria-Beograd, 70, no. 1 (2020):1-36,
https://doi.org/10.2478/acve-2020-0001 . .

TY  - JOUR
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Durdević, Biljana
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1769
AB  - Peripheral nerve sheath tumors (PNSTs) comprise a heterogeneous group of neoplasms originating from the elements of the nerve sheath. They are divided into two forms: benign and malignant PNST. Both benign and malignant PNSTs are not very common in domestic animals but they are reported in different animal species. Histologically, PNSTs are composed predominantly of spindle cells arranged in bundles, whorls and sheets, with a different number of pleomorphic cells and mitotic figures. The aim of this study was a reclassification of 17 dog tumor samples initially diagnosed with peripheral nerve sheath tumors using histopathological analysis. The main criterion for reclassification was immunohistochemical positivity for various antigens. PNSTs are often histologically very similar to other spindle cell tumors and immunohistochemistry is required for differential diagnosis. PNSTs generally express vimentin, S-100 protein, glial fibrillary acidic protein (GFAP), collagen IV and laminin. Four tumor samples were positive to muscular marker alpha-SMA and vimentin and negative for S-100 protein and desmin. The spindle cells whirling around some blood vessels were observed in these tumors so they were reclassified as perivascular wall tumors (PWTs). The other 13 tumors were S-100 protein and vimentin positive and alpha-SMA and desmin negative, thus classified as PNST. The use of the immunohistochemical panel is necessary for distinguishing PNSTs from PWTs in routine diagnostics.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors
VL  - 69
IS  - 3
SP  - 290
EP  - 299
DO  - 10.2478/acve-2019-0024
ER  - 

@article{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Durdević, Biljana and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Peripheral nerve sheath tumors (PNSTs) comprise a heterogeneous group of neoplasms originating from the elements of the nerve sheath. They are divided into two forms: benign and malignant PNST. Both benign and malignant PNSTs are not very common in domestic animals but they are reported in different animal species. Histologically, PNSTs are composed predominantly of spindle cells arranged in bundles, whorls and sheets, with a different number of pleomorphic cells and mitotic figures. The aim of this study was a reclassification of 17 dog tumor samples initially diagnosed with peripheral nerve sheath tumors using histopathological analysis. The main criterion for reclassification was immunohistochemical positivity for various antigens. PNSTs are often histologically very similar to other spindle cell tumors and immunohistochemistry is required for differential diagnosis. PNSTs generally express vimentin, S-100 protein, glial fibrillary acidic protein (GFAP), collagen IV and laminin. Four tumor samples were positive to muscular marker alpha-SMA and vimentin and negative for S-100 protein and desmin. The spindle cells whirling around some blood vessels were observed in these tumors so they were reclassified as perivascular wall tumors (PWTs). The other 13 tumors were S-100 protein and vimentin positive and alpha-SMA and desmin negative, thus classified as PNST. The use of the immunohistochemical panel is necessary for distinguishing PNSTs from PWTs in routine diagnostics.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors",
volume = "69",
number = "3",
pages = "290-299",
doi = "10.2478/acve-2019-0024"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M., Durdević, B.,& Aleksić-Kovačević, S.. (2019). Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 290-299.
https://doi.org/10.2478/acve-2019-0024

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Durdević B, Aleksić-Kovačević S. Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors. in Acta Veterinaria-Beograd. 2019;69(3):290-299.
doi:10.2478/acve-2019-0024 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Durdević, Biljana, Aleksić-Kovačević, Sanja, "Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors" in Acta Veterinaria-Beograd, 69, no. 3 (2019):290-299,
https://doi.org/10.2478/acve-2019-0024 . .

TY  - CONF
AU  - Nešić, Slađan
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Vučićević, Ivana
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3053
AB  - Клостридијалне инфекције животиња проузроковане су анаеробним
бактеријама из рода Clostridium. Клостридије су у спољашњој средини широко
распрострањене и веома отпорне бактерије, јер у неповољним условима
стварају споре које могу дуго да преживе. Такође, оне су нормални становници
дигестивног система животиња и људи. Код здравих животиња најчешће долази
до појаве инфекције приликом промене режима исхране, код повреда или
слабљења имунитета. Постоји велики број различитих врста бактерија из рода
Clostridium, али нису сви проузроковачи болести. Патогене врсте клостридија
проузрокују поремећаје здравственог стања животиња, најчешће са фаталним
исходом. Стопа морталитета код клостридијалних инфекција је висока. Због те
чињенице у фармском одгоју животиња могу да настану велики економски
губици. Најзначајније патогене врсте бактерија из рода Clostridium су: Cl.
perfrigens, Cl. chauvoei, Cl. haemoliticum, Cl. septicum, Cl. novyi, Cl. piliformis, Cl.
tetani и Cl. botulinum. Већина патогених сојева може самостално да проузрокује
настанак болести, али у неким случајевима то су мешовите инфекције
различитим врстама клостридија. Патогено дејство клостридије остварују након
умножавања и ослобађања различитих типова токсина, који могу да
проузрокују крвављења и некротичне промене локално или системски. У неким
случајевима долази до накупљања гасова у ткивима и органима. Бактеријски
токсини могу да изазову ћелијску смрт индиректно, смањењем оксигенације
ткива, или директно, оштећењем мембране ћелија у ткивима. Животиње
угињавају услед масивних некроза и крвављења праћених запаљењем у ткивима
и органима. У зависности од проузроковача некротично-хеморагичне промене
и запаљења могу да настану најчешће у дигестивном систему, јетри, централном
нервном систему, срцу и скелетној мускулатури различитих животињских
врста. Сваки тип клостридијалних токсина утиче на појаву карактеристичних
морфолошких промена код појединих животињских врста.
AB  - Clostridial infections in animals are caused by anaerobic bacteria of the genus
Clostridium. Clostridium species are widely distributed in environment and highly
resistant bacteria, due to their ability to form a spore and survive for a long time. They
represent residential microorganisms in the gastrointestinal tract of animals and
humans. In healthy animals, change in diet, injuries or weakened immune system most
commonly trigger the onset clostridial infections. There is a large number of different
species of genus Clostridium, but not all species of clostridia cause disease.
Pathogenic species of clostridia cause animal health disorders, most often with a fatal
outcome. The rate of mortality in clostridial infections is high. Due to this fact in the
farm breeding of animals, large economic losses can occur. The most important
pathogenic bacteria of the genus Clostridium are: Cl. perfrigens, Cl. chauvoei, Cl.
haemoliticum, Cl. septicum, Cl. novyi, Cl. pyliformis, Cl. tetani and Cl. botulinum.
Most commonly, infection is caused by single bacterial species, but sometimes it is
caused by variety of pathogenic clostridial species. The pathogenic effect of clostridia
occurs after the multiplication and release of different types of toxins, which can cause
local or systemic bleeding and necrotic changes. In some cases, gases accumulate in
the tissues and organs. The bacterial toxins can cause cell death indirectly, by reducing
tissue oxygenation, or directly by damaging plasma membrane of cells in tissues.
Animals could die due to massive necrosis and bleeding accompanied by
inflammation in tissues and organs. Depending on the causative factor, necrotichemorrhagic changes and inflammation can occur most often in the gastrointestinal
tract, liver, central nervous system, heart and skeletal muscle of many animal species.
Each type of clostridial toxins cause the characteristic morphological changes in
certain animal species.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta
T1  - Morphological changes due to clostridial infections in different animal species
SP  - 158
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3053
ER  - 

@conference{
author = "Nešić, Slađan and Marinković, Darko and Kukolj, Vladimir and Vučićević, Ivana and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Клостридијалне инфекције животиња проузроковане су анаеробним
бактеријама из рода Clostridium. Клостридије су у спољашњој средини широко
распрострањене и веома отпорне бактерије, јер у неповољним условима
стварају споре које могу дуго да преживе. Такође, оне су нормални становници
дигестивног система животиња и људи. Код здравих животиња најчешће долази
до појаве инфекције приликом промене режима исхране, код повреда или
слабљења имунитета. Постоји велики број различитих врста бактерија из рода
Clostridium, али нису сви проузроковачи болести. Патогене врсте клостридија
проузрокују поремећаје здравственог стања животиња, најчешће са фаталним
исходом. Стопа морталитета код клостридијалних инфекција је висока. Због те
чињенице у фармском одгоју животиња могу да настану велики економски
губици. Најзначајније патогене врсте бактерија из рода Clostridium су: Cl.
perfrigens, Cl. chauvoei, Cl. haemoliticum, Cl. septicum, Cl. novyi, Cl. piliformis, Cl.
tetani и Cl. botulinum. Већина патогених сојева може самостално да проузрокује
настанак болести, али у неким случајевима то су мешовите инфекције
различитим врстама клостридија. Патогено дејство клостридије остварују након
умножавања и ослобађања различитих типова токсина, који могу да
проузрокују крвављења и некротичне промене локално или системски. У неким
случајевима долази до накупљања гасова у ткивима и органима. Бактеријски
токсини могу да изазову ћелијску смрт индиректно, смањењем оксигенације
ткива, или директно, оштећењем мембране ћелија у ткивима. Животиње
угињавају услед масивних некроза и крвављења праћених запаљењем у ткивима
и органима. У зависности од проузроковача некротично-хеморагичне промене
и запаљења могу да настану најчешће у дигестивном систему, јетри, централном
нервном систему, срцу и скелетној мускулатури различитих животињских
врста. Сваки тип клостридијалних токсина утиче на појаву карактеристичних
морфолошких промена код појединих животињских врста., Clostridial infections in animals are caused by anaerobic bacteria of the genus
Clostridium. Clostridium species are widely distributed in environment and highly
resistant bacteria, due to their ability to form a spore and survive for a long time. They
represent residential microorganisms in the gastrointestinal tract of animals and
humans. In healthy animals, change in diet, injuries or weakened immune system most
commonly trigger the onset clostridial infections. There is a large number of different
species of genus Clostridium, but not all species of clostridia cause disease.
Pathogenic species of clostridia cause animal health disorders, most often with a fatal
outcome. The rate of mortality in clostridial infections is high. Due to this fact in the
farm breeding of animals, large economic losses can occur. The most important
pathogenic bacteria of the genus Clostridium are: Cl. perfrigens, Cl. chauvoei, Cl.
haemoliticum, Cl. septicum, Cl. novyi, Cl. pyliformis, Cl. tetani and Cl. botulinum.
Most commonly, infection is caused by single bacterial species, but sometimes it is
caused by variety of pathogenic clostridial species. The pathogenic effect of clostridia
occurs after the multiplication and release of different types of toxins, which can cause
local or systemic bleeding and necrotic changes. In some cases, gases accumulate in
the tissues and organs. The bacterial toxins can cause cell death indirectly, by reducing
tissue oxygenation, or directly by damaging plasma membrane of cells in tissues.
Animals could die due to massive necrosis and bleeding accompanied by
inflammation in tissues and organs. Depending on the causative factor, necrotichemorrhagic changes and inflammation can occur most often in the gastrointestinal
tract, liver, central nervous system, heart and skeletal muscle of many animal species.
Each type of clostridial toxins cause the characteristic morphological changes in
certain animal species.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta, Morphological changes due to clostridial infections in different animal species",
pages = "158-159",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3053"
}

Nešić, S., Marinković, D., Kukolj, V., Vučićević, I., Aničić, M.,& Aleksić-Kovačević, S.. (2019). Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 158-159.
https://hdl.handle.net/21.15107/rcub_veterinar_3053

Nešić S, Marinković D, Kukolj V, Vučićević I, Aničić M, Aleksić-Kovačević S. Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:158-159.
https://hdl.handle.net/21.15107/rcub_veterinar_3053 .

Nešić, Slađan, Marinković, Darko, Kukolj, Vladimir, Vučićević, Ivana, Aničić, Milan, Aleksić-Kovačević, Sanja, "Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):158-159,
https://hdl.handle.net/21.15107/rcub_veterinar_3053 .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Đurđević, Biljana
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3054
AB  - Циљ овог испитивања је рекласификација 17 узорака тумора паса који су
првобитном хистопатолошком анализом дијагностиковани као тумори овојница
периферних нерава. Главни критеријум за рекласификацију била је
имунохистохемијска позитивност на различите антигене.
Тумори овојница периферних нерава обухватају хетерогену групу неоплазми
које потичу од елемената који граде нервне овојнице. Ове неоплазме се могу
поделити у две групе: бенигне и малигне туморе овојница периферних нерава.
Бенигни тумори овојница периферних нерава нису заступљени у великом броју
код домацћих животиња, али је њихова појава забележена код различитих
животињских врста, као што су мачка, коњ, свиње и козе, при чему се најчешће
јавља код паса и говеда. Малигни тумори овојница периферних нерава се такође
релативно ретко дијагностикују код животиња и чине 27% свих тумора нервног
система. Хистолошки, бенигни тумори овојница периферних нерава су обично
добро ограничене, инкапсулисане новотворевине, претежно састављене од
вретенастих ћелија распоређених у снопове, палисадно или вртложно. Малигни
тумори овојница периферних нерава су неинкапсулисани и карактерише их
изражен полиморфизам ћелија распоређених у виду снопова или спирала са
бројним митотским фигурама.
Тумори овојница периферних нерава су често хистолошки врло слични другим
туморима насталим пролиферацијом ћелија вретенастог облика, те је стога
неопходно урадити имунохистохемијска бојења ради постављања
диференцијалне дијагнозе. Ове неоплазме обично показују имунохистохемијску позитивност на виментин, S-100, глијални фибриларни кисели
протеин, колаген типа IV и ламинин.
Четири тумора су била имунохистохемијски позитивна на мишићни маркер αSMA и виментин, а негативни на S-100 и дезмин. Присуство вретенастих ћелија
које стварају вртлоге око неколико крвних судова уочене су у овим туморима,
те су они рекласификовани у периваскуларне туморе. Осталих 13 тумора били
су S-100 и виментин позитивни, а α-SMA и дезмин негативни, чиме је потврђена
првобитна дијагноза да се ради о туморима овојница периферних нерава.
Разликовање тумора овојница периферних нерава и периваскуларних тумора
није једноставно ни поред примене имунохистохемијског панела.
AB  - The aim of this study was reclassification of 17 dog tumor samples initially diagnosed
with peripheral nerve sheath tumors (PNST) using histopathological analysis. The
main criteria for reclassification were immunochistohemical positivity on various
antigens.
Peripheral nerve sheath tumors comprise a heterogeneous group of neoplasms
originating from the elements of the nerve sheath. They are divided into two forms:
benign and malignant PNST. Benign PNSTs are not very common in domestic
animals but they are reported in different animal species, such as cat, horse, pig and
goat and most frequently are seen in dogs and cattle. Likewise, malignant PNSTs are
rare neoplasm in animals and they account for 27% of all nervous system tumors.
Histologically, benign PNSTs are usually well circumscribed, encapsulated and
composed predominantly of spindle cells arranged in bundles and sheets, while
malignant PNSTs are encapsulated and consist of highly pleomorphic cells with
numerous mitotic figures arranged in fascicles, whorls or sheets.
PNSTs are often histologically very similar to other spindle cell tumors and
immunohistochemistry is required for differential diagnosis. PNSTs generally express
vimentin, S-100, glial fibrillary acidic protein, collagen IV and laminin.
Four tumor samples were positive on muscular marker α-SMA and vimentin and
negative for S-100 and desmin. The spindle cells whirling around some blood vessels
were observed in these tumors so they are reclassified as perivascular tumors (PWTs).
The other 13 tumors were S-100 and vimentin positive and α-SMA and desmin
negative, thus classified as PNST.
Even after using the immunohistochemical panel, distinguishing between PNSTs and
PWTs is a quite difficult.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Re-evaluacija tumora ovojnica perifernih nerava pasa
T1  - Reevaluation of canine peripheral nerve sheath tumors (pnst)
SP  - 176
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3054
ER  - 

@conference{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Đurđević, Biljana and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Циљ овог испитивања је рекласификација 17 узорака тумора паса који су
првобитном хистопатолошком анализом дијагностиковани као тумори овојница
периферних нерава. Главни критеријум за рекласификацију била је
имунохистохемијска позитивност на различите антигене.
Тумори овојница периферних нерава обухватају хетерогену групу неоплазми
које потичу од елемената који граде нервне овојнице. Ове неоплазме се могу
поделити у две групе: бенигне и малигне туморе овојница периферних нерава.
Бенигни тумори овојница периферних нерава нису заступљени у великом броју
код домацћих животиња, али је њихова појава забележена код различитих
животињских врста, као што су мачка, коњ, свиње и козе, при чему се најчешће
јавља код паса и говеда. Малигни тумори овојница периферних нерава се такође
релативно ретко дијагностикују код животиња и чине 27% свих тумора нервног
система. Хистолошки, бенигни тумори овојница периферних нерава су обично
добро ограничене, инкапсулисане новотворевине, претежно састављене од
вретенастих ћелија распоређених у снопове, палисадно или вртложно. Малигни
тумори овојница периферних нерава су неинкапсулисани и карактерише их
изражен полиморфизам ћелија распоређених у виду снопова или спирала са
бројним митотским фигурама.
Тумори овојница периферних нерава су често хистолошки врло слични другим
туморима насталим пролиферацијом ћелија вретенастог облика, те је стога
неопходно урадити имунохистохемијска бојења ради постављања
диференцијалне дијагнозе. Ове неоплазме обично показују имунохистохемијску позитивност на виментин, S-100, глијални фибриларни кисели
протеин, колаген типа IV и ламинин.
Четири тумора су била имунохистохемијски позитивна на мишићни маркер αSMA и виментин, а негативни на S-100 и дезмин. Присуство вретенастих ћелија
које стварају вртлоге око неколико крвних судова уочене су у овим туморима,
те су они рекласификовани у периваскуларне туморе. Осталих 13 тумора били
су S-100 и виментин позитивни, а α-SMA и дезмин негативни, чиме је потврђена
првобитна дијагноза да се ради о туморима овојница периферних нерава.
Разликовање тумора овојница периферних нерава и периваскуларних тумора
није једноставно ни поред примене имунохистохемијског панела., The aim of this study was reclassification of 17 dog tumor samples initially diagnosed
with peripheral nerve sheath tumors (PNST) using histopathological analysis. The
main criteria for reclassification were immunochistohemical positivity on various
antigens.
Peripheral nerve sheath tumors comprise a heterogeneous group of neoplasms
originating from the elements of the nerve sheath. They are divided into two forms:
benign and malignant PNST. Benign PNSTs are not very common in domestic
animals but they are reported in different animal species, such as cat, horse, pig and
goat and most frequently are seen in dogs and cattle. Likewise, malignant PNSTs are
rare neoplasm in animals and they account for 27% of all nervous system tumors.
Histologically, benign PNSTs are usually well circumscribed, encapsulated and
composed predominantly of spindle cells arranged in bundles and sheets, while
malignant PNSTs are encapsulated and consist of highly pleomorphic cells with
numerous mitotic figures arranged in fascicles, whorls or sheets.
PNSTs are often histologically very similar to other spindle cell tumors and
immunohistochemistry is required for differential diagnosis. PNSTs generally express
vimentin, S-100, glial fibrillary acidic protein, collagen IV and laminin.
Four tumor samples were positive on muscular marker α-SMA and vimentin and
negative for S-100 and desmin. The spindle cells whirling around some blood vessels
were observed in these tumors so they are reclassified as perivascular tumors (PWTs).
The other 13 tumors were S-100 and vimentin positive and α-SMA and desmin
negative, thus classified as PNST.
Even after using the immunohistochemical panel, distinguishing between PNSTs and
PWTs is a quite difficult.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Re-evaluacija tumora ovojnica perifernih nerava pasa, Reevaluation of canine peripheral nerve sheath tumors (pnst)",
pages = "176-177",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3054"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M., Đurđević, B.,& Aleksić-Kovačević, S.. (2019). Re-evaluacija tumora ovojnica perifernih nerava pasa. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 176-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3054

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Đurđević B, Aleksić-Kovačević S. Re-evaluacija tumora ovojnica perifernih nerava pasa. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:176-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3054 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Đurđević, Biljana, Aleksić-Kovačević, Sanja, "Re-evaluacija tumora ovojnica perifernih nerava pasa" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):176-177,
https://hdl.handle.net/21.15107/rcub_veterinar_3054 .

TY  - CONF
AU  - Aničić, Milan
AU  - Juntes, Polona
AU  - Vasković, Nikola
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
AU  - Marinković, Darko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2920
PB  - Elsevier
C3  - Udruženi evropski kongres Evropskog udruženja veterinarskih patologa (ESVP) i Evropskog koledža veterinarskih patologa (ECVP), Kluž-Napoka, Rumunija, 5 – 8. septembar, 2018, ESVP, ESTP and ECVP Proceedings 2018, Journal of Comparative Pathology, 2019
T1  - Morphological and immunohistochemical characteristics of spontaneous distemper infection in the brain of red foxes in Serbia
SP  - 142
EP  - 142
DO  - 10.1016/j.jcpa.2018.10.135
ER  - 

@conference{
author = "Aničić, Milan and Juntes, Polona and Vasković, Nikola and Nešić, Slađan and Vučićević, Ivana and Aleksić-Kovačević, Sanja and Marinković, Darko",
year = "2019",
publisher = "Elsevier",
journal = "Udruženi evropski kongres Evropskog udruženja veterinarskih patologa (ESVP) i Evropskog koledža veterinarskih patologa (ECVP), Kluž-Napoka, Rumunija, 5 – 8. septembar, 2018, ESVP, ESTP and ECVP Proceedings 2018, Journal of Comparative Pathology, 2019",
title = "Morphological and immunohistochemical characteristics of spontaneous distemper infection in the brain of red foxes in Serbia",
pages = "142-142",
doi = "10.1016/j.jcpa.2018.10.135"
}

Aničić, M., Juntes, P., Vasković, N., Nešić, S., Vučićević, I., Aleksić-Kovačević, S.,& Marinković, D.. (2019). Morphological and immunohistochemical characteristics of spontaneous distemper infection in the brain of red foxes in Serbia. in Udruženi evropski kongres Evropskog udruženja veterinarskih patologa (ESVP) i Evropskog koledža veterinarskih patologa (ECVP), Kluž-Napoka, Rumunija, 5 – 8. septembar, 2018, ESVP, ESTP and ECVP Proceedings 2018, Journal of Comparative Pathology, 2019
Elsevier., 142-142.
https://doi.org/10.1016/j.jcpa.2018.10.135

Aničić M, Juntes P, Vasković N, Nešić S, Vučićević I, Aleksić-Kovačević S, Marinković D. Morphological and immunohistochemical characteristics of spontaneous distemper infection in the brain of red foxes in Serbia. in Udruženi evropski kongres Evropskog udruženja veterinarskih patologa (ESVP) i Evropskog koledža veterinarskih patologa (ECVP), Kluž-Napoka, Rumunija, 5 – 8. septembar, 2018, ESVP, ESTP and ECVP Proceedings 2018, Journal of Comparative Pathology, 2019. 2019;:142-142.
doi:10.1016/j.jcpa.2018.10.135 .

Aničić, Milan, Juntes, Polona, Vasković, Nikola, Nešić, Slađan, Vučićević, Ivana, Aleksić-Kovačević, Sanja, Marinković, Darko, "Morphological and immunohistochemical characteristics of spontaneous distemper infection in the brain of red foxes in Serbia" in Udruženi evropski kongres Evropskog udruženja veterinarskih patologa (ESVP) i Evropskog koledža veterinarskih patologa (ECVP), Kluž-Napoka, Rumunija, 5 – 8. septembar, 2018, ESVP, ESTP and ECVP Proceedings 2018, Journal of Comparative Pathology, 2019 (2019):142-142,
https://doi.org/10.1016/j.jcpa.2018.10.135 . .